Hybridisation Incubators

Transcription

1 Techne Hybridisation Incubators Techne HB-1D and Hybrigene hybridisation incubators replace the cumbersome shaker bath and plastic bag method. At least five 200x200mm membranes can be probed in the large hybridisation tube in DNA experiments. Hybridisation can be performed using a minimal volume of 5ml, an 80% reduction over conventional systems, reducing expense and improving signal. The updated HB-1D now has adjustable rotation speed and is supplied complete with a stainless steel drip tray and three large hybridisation tubes. The capacity to run up to five membranes in each tube, without any increase in background, means that a large number of hybridisations can be performed simultaneously. The unit will accept up to 6 large or small hybridisation tubes or 24 mini tubes (6x4). The unit has digital set and display of temperature. The Hybrigene is a compact, stackable alternative to the HB-1D and is the perfect choice when quality is important and throughput is not a priority. The Hybrigene is stackable which makes it ideal for today s modern, overcrowded laboratory - up to 3 ovens can be stacked saving on valuable space. Two Hybrigenes can be stacked under most laboratory benches. The unit will accept up to 4 large tubes or small tubes or 16 mini tubes (4x4). Tube holders with clips are available to carry 64x15ml and 32x50ml conical tubes. Accessories for the HB-1D and Hybrigene include adjustable rocking platforms which fit on the base of the units, allowing hybridization tubes to be used above. Up to 2 (Hybrigene) or 3 (HB-1D) static stainless steel mesh shelves can also be placed into the tube locators, allowing the unit to be used as a simple incubator. Slot-in tubes for easy loading Adjustable feet for levelling on uneven surfaces Removable drip tray for easy cleaning Double-glazed door for durable protection Radioactive safe; protective casing and non-drip tube design minimises risk to the user Model Hybrigene HB-1D Temperature range, C Ambient +10 to 80 Ambient +10 to 100 Temperature stability in the chamber, C <±1.0 <±1.0 Temperature stability in the tubes, C <±0.1 <±0.1 Temperature uniformity in the chamber, C <±1.5 <±1.5 Temperature uniformity in the tubes, C <±0.5 <±1.0 Temperature setting Digital Digital Temperature display Digital LED Digital LED Set point resolution, C Absolute accuracy, C <± 0.3 <± 0.3 Speed of rotation (adjustable), rpm 0, , 5-20 Rocking platform (option), rpm 5-20 or or Capacity: Max number of tubes 4 large/small or 16 mini tubes 6 large/small or 24 mini tubes Capacity: Membranes per tube At least 5 (large tube) At least 5 (large tube) Probe As little as 5ml required As little as 5ml required Net weight, kg External dimensions (dxwxh), mm 355x383x x385x (tube holder sold separately) stacked (tube holder sold separately) HB-1D hybridisation incubator complete with three large POA 80x200mm (dxl) hybridisation tubes Hybrigene hybridisation incubator complete with four large POA 80x240mm (dxl) hybridisation tubes HB-1D and Hybrigene Accessories Hybridisation tubes and tube carriers All Techne hybridisation tubes are manufactured from heavy-walled borosilicate glass. 3 sizes are available - small, mini and large Durable - thick borosilicate glass Easy pour - non drip, safe and convenient Sealing ring - O ring sealed end caps prevent leakage Broken thread on screw cap - easy opening with no vacuum problems Tube assemblies - adaptors to hold multiple small glass tubes to increase capacity 501

2 Techne for HB-1D for HB-1D with tube carrier Hybridisation Incubators, continued Cat. No. Description Dims Total Type of end caps description Pack Price (dia.xl), tubes mm per unit Hybridisation tubes and tube carrier for HB-1D * Large glass tube 80x200 6 Push-on end caps 1 POA Large glass tube 80x200 6 No end caps 1 POA no cap Small glass tube 44x200 6 Single screw-on end cap. 1 POA Tube has end pip Mini glass tube 32x205 6x4 Single screw-on end cap, 4 POA assembly requires carrier Tube carrier For 32x205mm tubes ( ) 1 POA Sealing rings for push-on end caps for operation up to 100 C (two required) 1 POA Hybridisation tubes and tube carrier for Hybrigene * Large glass tube 80x240 4 Push on end caps 1 POA Large glass tube 80x240 4 No end caps 1 POA no cap Small glass tube 44x240 4 Single screw-on end cap. 1 POA Tube has end pip Mini glass tube 32x205 4x4 Screw-on end cap, requires 4 POA assembly carrier Tube carrier For 32x205mm tubes ( ) 1 POA Small glass tube 44x220 4x3 Single screw-on end cap, without 1 POA assembly end pip, requires carrier Tube carrier For 44x220mm tubes ( ) 1 POA Complete assembly 44x220 4x3 Carrier ( ) and tubes 3 POA complete, 3x small (44x200mm) tubes without end pips ( ) ml Tube holder 4x16 Carrier with clips to hold 16x15ml 1 POA tubes (approx. diameter 16.5mm) ml Tube holder 4x8 Carrier with clips to hold 8x50ml 1 POA tubes (approx. diameter 29mm) Sealing rings for push-on end caps for operation up to 100 C (two required) 1 POA * The sealing rings supplied with these tubes are suitable only for operation up to 70 C. For operation up to 100 C use Cat. No Rocking Platform for HB-1D and Hybrigene Fits on the base of the unit still allowing hybridisation tubes to be placed above. Enables membranes to be processed using a wave motion which is particularly suitable for RNA work. Available in fast or slow speeds which range from 5 to 60 oscillations per minute Rocking platform for HB-1D, slow 5-20 oscillations/min POA Rocking platform for HB-1D, fast oscillations/min POA Rocking platform for Hybrigene, slow 5-20 oscillations/min POA Rocking platform for Hybrigene, fast oscillations/min POA Shelf for HB-1D and Hybrigene Up to 2 (Hybrigene) or 3 (HB-1D) static stainless steel mesh shelves can be placed into the tube locators allowing the unit to be used as a simple non-motion incubator. Each shelf holds up to 4kg Stainless steel mesh shelf for HB-1D POA Stainless steel mesh shelf for Hybrigene POA Hybridisation Tube Holder/Rack for HB-1D and Hybrigene Provides added safety for storing tubes while not in use. The tube holder doubles as a support in holding tubes during loading, suitable for three large and three small tubes and end caps Hybridisation tube holder POA 502

3 UVP Hybridisation Incubators UVP has developed a line of hybridisation ovens designed to create the ideal environment for nucleic acid hybridisation. The series includes the HC-3000 HybriCycler, HB-500 Minidizer, HL-2000 HybriLinker, HB-1000 Hybridizer and the HM-4000 Multidizer. Hybridisation incubators are ideal for procedures involved in Southern, Northern and in-situ hybridisation. UVP s hybridisation incubators include precise microprocessor temperature controls with rotation speed control and variable bottle positioning for dynamic mixing capability. This is needed for cdna library screenings, primer synthesis and nucleic acid hybridisation. Accurate temperature and motion controls Easy to clean interior stainless steel construction UVP manufactured quality and reliability Wide selection to meet individual user or laboratory budgets and requirements HB-500 Minidizer The UVP Minidizer is designed as a personal benchtop unit ideal for use in laboratories with low use requirements or space constraints. Consistent, repeatable results are achieved with microprocessor controls. The chamber is of stainless steel fabrication with a 95mm thick ß-blocking acrylic cover. The cover swings down for access to the carousel. Pop the carousel out of the chamber for easy insertion or removal of bottles. Speed, rpm 12 Heater, W 500 Temperature range, C Ambient to 80 ±0.3 inside oven/±0.1 inside bottles Uniformity, C ±1.0 Bottle/tube capacity Four 35x150mm, four 50ml or eight 15ml conical tubes Dimensions (wxhxd), mm 229x330x203 Weight, kg HB-500 Minidizer hybridisation oven POA For accessories see page HC-3000 HybriCycler UVP s HC-3000 HybriCycler has a unique circular design, creating optimal conditions for temperature uniformity and stability. The wraparound 95mm thick ß-blocking acrylic chamber allows complete visibility of bottles without opening the door. The lightweight, acrylic carousel is easily removed for quick access to bottles and rotates on the motorised rollers. For larger bottles, simply remove the carousel and place the bottles directly on the rollers. The unit features microprocessor operation for precise mixing capability. Temperature and speed controls are operated by the touch sensitive keypad and displayed on the LED screen. The base and control panel housing are manufactured with a scratch-resistant, powder-coated epoxy finish for easy cleaning and maintenance. The unique design allows for spills to drip into a pullout tray for easy cleaning and decontamination. Speed, rpm 11 Heater, W 500 Temperature range, C Ambient to 80 ±0.3 inside oven/0.1 inside bottles Uniformity, C ±1.0 Bottle/tube capacity Eight 35x150mm, four 35x300mm or eight 50ml conical tubes Dimensions (wxhxd), mm 508x267x411 Weight, kg HC-3000 HybriCycler hybridisation oven POA For accessories see page (tubes sold separately) 503

4 UVP (supplied with one large bottle) HB-1000 Hybridizer The HB-1000 can be stacked to make the best use of laboratory space while performing multiple functions at varying temperatures and speeds. This incubator features a microprocessor-controlled rotary carousel wheel that is removable to accommodate a rocker plate for all hybridisation protocols in one benchtop unit. A touch-sensitive keypad and microprocessor provides accurate temperature control from ambient to 99.9 C. The LED readout displays the current temperature inside the chamber. An internal circulating fan and powerful 1250 watt power supply ensure uniform heat distribution throughout the chamber. Two different hybridiser bottle sizes are available for the HB-1000 and combinations of both size bottles may be used at the same time. The HB-1000 is manufactured with a stainless steel internal construction. A removable protective tray under the rocker or carousel permits easy cleaning of spilled media. The door latches securely to prevent heated air escaping from the chamber. The door s tempered glass window is sealed and allows for visibility of bottles. Includes one large hybridisation bottle. Speed, rpm Heater, W 1250 Temperature range, C Ambient to 99.9 ±0.3 inside oven/±0.1 inside bottles Uniformity, C ±1.0 Bottle capacity Twenty 35x150mm, ten 35x300mm Dimensions (wxhxd), mm 445x406x381 Weight, kg HB-1000 Hybridizer hybridisation oven POA For accessories see below (supplied with one large bottle) HM-4000 Multidizer The Multidizer is designed with two compartments and a variety of motion options. Both compartments operate independently, allowing for hybridisation and blotting procedures requiring different settings. The Multidizer has microprocessor temperature and motion controls for precision heating and motion. The lower chamber features the same operating controls and bottle capacity as the HB-1000 (includes one large hybridisation bottle). The upper chamber operates with a roller action to operate a reciprocating shaker tray (included) or an optional orbital rocking tray for accommodating a variety of containers. ±0.3 inside oven/±0.1 inside bottles Uniformity, C ±0.1 Upper Chamber Temperature range, C Ambient to 80 Heater, W 500 Shaker tray speed cycles/min Orbital shaker cycles/sec. Angle ±0 to ±7 Carousel, rpm Lower chamber Temperature range, C Ambient to 99.9 Heater, W 1250 Speed, rpm Bottle capacity Twenty 35x150mm, ten 35x300mm Dimensions (wxhxd), mm 445x725x381 Weight, kg HM-4000 Multidizer hybridisation oven POA Accessories UVP hybridisation ovens Bottle, small (35x150mm) POA Bottle, large (35x300mm) POA Rocker tray, 318x254mm, for use with HB-1000 and HM-4000 POA Orbital rocker tray, 305x191mm, for use with HM-4000 POA 504

5 Quanta Biotech Q-Hyb 10 Hybridisation Incubator The Q-Hyb 10 is a high quality, versatile hybridisation incubator. Its rotisserie* has the capacity to conduct up to 20 independent probe hybridisations, each with up to seven separate membranes. Alternatively, the rotisserie can be interchanged with a shaking platform* to facilitate large scale hybridisation or batch washing protocols. The rotisserie and shaking platform can be interchanged in less than a minute allowing researchers to quickly adapt the incubator to the needs of their individual protocols and employ strategies utilising both accessories. This capacity and flexibility enables the incubator to service multiple users and act as a core hybridisation facility in even the most demanding research laboratory. Other features include PID control for reproducible results and an over-temperature safety cut-out. A range of hybridisation bottles is available, made from borosilicate glass. Large capacity rotisserie accommodates 10 large or 20 small bottles Wide temperature range (ambient +5 C to 95 C) for membrane stripping protocols Timer with alarm for accurate step timing in multi step protocols Interchangeable rotisserie, shaking tray and up to 4 incubation shelves* Index loading system for controlled experimental set up and rapid change of washing fluids Rotisserie/shaker speed 5 to 20rpm Easy to clean stainless steel interior and door to ensure rapid and complete decontamination Large window to view experiments High precision PID control for reproducible experiments Bottle to bottle uniformity ±0.1 C Stackable (up to 3 high) to minimise wastage of laboratory space Over-temperature safety cut-out shown with 2 incubator shelves ( ) * These are not supplied with the incubator and must be ordered separately. Temperature range, C ambient +5 to 95 Temperature accuracy, C ±0.5 Bottle to bottle uniformity, C ±0.1 Over-temperature cut-out Set at 100 C Temperature display LED Display resolution, C 0.1 No. of rotisseries, supplied/max. 0/1 Rotisserie/shaker speed, rpm 5 to 20 No. of shelves supplied/max. 0/4 Internal dimensions (wxdxh), mm 352x260x250 Overall dimensions (wxdxh), mm 480x353x327 Net weight, kg 18 Electrical supply 230V, 50Hz, 350W Q-Hyb10 Hybridisation incubator* POA * Rotisserie, shaking tray, incubation shelves and hybridisation bottles must be ordered separately. Please see below for details. Accessories Rotisserie pack includes: rotisserie for 10x 300mm long, 35mm diameter POA bottles or 20x 150mm long, 35mm diameter bottles. Supplied with 2 large hybridisation bottles and pack of large mesh Incubator shelf pack includes: 2x stainless steel incubation shelves POA (348x15x233 wxhxd, mm) Shaking tray pack includes: 1x stainless steel shaking tray POA (310x15x210 wxhxd, mm) Large hybridisation bottle, borosilicate glass (300mm length, 35mm diameter) POA Small hybridisation bottle, borosilicate glass (150mm length, 35mm diameter) POA Large nylon mesh (250x250mm), pack of 10 POA Small nylon mesh (130x130mm), pack of 10 POA Safety sheath to insulate and protect hands during bottle opening POA

6 Stuart SI30H Hybridisation Incubator and Shaker A very versatile 20 litre hybridisation incubator and combined rocker shaker which only requires a minimum of bench space. The incubator temperature is accurately set via the easy to use encoder controls and displayed by a bright LED. Forced air circulation combined with microprocessor control means outstanding temperature uniformity. The safety first design includes an over-temperature protection system that automatically tracks the set temperature and controls the heater in the event of a fault. The smart system displays error codes indicating a fault has occurred or if the set temperature has been interrupted. The incubator also cuts power to the motor if it overheats or stalls. The incubator has a stainless steel interior, whilst the door is constructed from a double glazed panel of smoked acrylic and polycarbonate to provide radiation protection. The up and over door mechanism gives excellent access to the incubator. Conversion of the unit to a rocker shaker is fast and easy. Simply remove the rotisserie and pull forward the platform located at the rear of the incubator. The gentle rocking action is ideal for the washing stage of most hybridisation procedures. Rocking speed is fully variable allowing optimisation depending on application. A range of hybridisation bottles made from tough Pyrex glass and leak proof PTFE faced screwcaps, are available. Compact design Rotisserie and rocker in one unit Up and over door for accessibility Accurate temperature control Advanced safety features Variable speed control Nominal capacity, L 20 Temperature range, C 8 to 80 Temperature fluctuation at 37 C, C ±0.5 Temperature variation, C ±0.5 Over temperature cut-out Yes Temperature display LED Display resolution, C 0.1 No. of rotisseries 1 Rotational speed, rpm 2 to 10 Shaker speed, oscillations/min 5 to 70 Internal dimensions (wxdxh), mm 250x230x200 Overall dimensions (wxdxh), mm 380x380x435 Net weight, kg 24 Electrical supply 230V, 50Hz, 350W SI30H Hybridisation oven/shaker supplied with 1 rotisserie POA SI20H/1 (7x40mm dia. bottles) Incubator is supplied without hybridisation bottles; these should be ordered separately. Accessories SI20H/1 Spare rotisserie for 7x40 mm diameter bottles POA SI20H/2 Accessory rotisserie for 2x75mm diameter bottles POA SI20H/7 Accessory rotisserie for 24x50ml tubes POA Please see page 507 for hybridisation bottles. 506

7 Accessories Hybridisation Bottles for Stuart S130H Hybridisation Incubator Bottle hybridisation minimises probe volumes, reduces reagent volumes and enhances signal intensity. Made from Pyrex borosilicate glass Stuart hybridisation bottles are robust and can easily withstand the temperatures of the most rigorous hybridisation techniques. The thermoplastic polyester caps are very rigid and will not distort during repeated heating in the incubator which can lead to leakage. The seal is made by a PTFE faced insert that covers the entire inside of the cap making very good contact with the glass thread. Available in three sizes, each bottle comes complete with cap and a care leaflet. Cat. No. Bottle Overall Outer Inter Wall thickness Price length, mm dia., mm dia., mm mm SI20H/ POA SI20H/ POA SI20H/ POA Micro-Array Slide Holder The micro-array slide holder is a low cost disposable, manufactured in polypropylene and designed to facilitate the manipulation of micro-array slides during various stages of the micro-array production, hybridisation and detection process. The holder is suitable for use with most common micro-array format glass slides (25x75mm) and enables the slide to be held securely inside a standard 50ml centrifuge tube. Slide washing, hybridisation and centrifuge drying can then all be conveniently accomplished. These micro-array slide holders withstand a range of temperatures between -80 C to +121 C. Cat. No. Description Colour Pack Price Micro-Array slide holder Cream 20 POA Micro-Array slide holder Green 20 POA Micro-Array slide holder Blue 20 POA Micro-Array slide holder Orange 20 POA Micro-Array Hybridisation Chamber The HC 2 micro-array hybridisation chamber is the first low cost, semi-disposable, truly reliable hybridisation chamber, designed for performing nucleic acid hybridisation on 25x75mm micro-array glass slides. The HC 2 micro-array hybridisation chamber enables micro-array slides to be hybridised conveniently, safely and reproducibly. The chambers can be used in air incubators or waterbaths. Each chamber incorporates a dual humidity system to prevent evaporation of the hybridisation buffer. Two reservoirs maintain a constant humidity while an absorbent paper is supplied to rapidly humidify the chamber at high hybridisation temperatures. Manufactured in an inert polymer with similar chemical resistant properties to that of polypropylene. The chambers have been tested at hybridisation temperatures up to 65 C and can be autoclaved if required. At either end of the chamber there are recessed wells to accommodate a small volume (approx. 600µl/well) of water or hybridisation buffer to humidify the chamber atmosphere to Tube sold separately Cat. No. Description Pack Price HC2 hybridisation chamber 2 POA Space absorbent paper 50 POA