OPERATOR S MANUAL. SterilSHIELD Product Protection Only Isolator MODEL SS500

Transcription

1 THE BAKER COMPANY OPERATOR S MANUAL SterilSHIELD Product Protection Only Isolator MODEL SS500 This manual includes information for installation, operation, maintenance and spare parts. We recommend that it be kept near the cabinet for ready reference.

2 THE BAKER COMPANY INTRODUCTION AND WELCOME It is a pleasure to welcome you to the growing number of customers who own and operate Baker cabinets and gloveboxes. As the inventors of the laminar flow biological safety cabinet and the leaders in the field, Baker people take special pride in providing a cabinet that is designed for maximum performance. Your new SterilSHIELD cabinet includes many unique features which are included to give you superior performance, simpler maintenance and lower life cycle cost. Your SterilSHIELD cabinet is designed for both product protection and value. In addition to the high quality you expect from all Baker equipment, this model has been ergonomically designed to provide the lab user with many exciting design features. The revolutionary ergonomic design will help prevent repetitive motion injury, reduce fatigue and lab accidents and enhance productivity. You will find your SterilSHIELD cabinet suitable for research and clinical diagnostic work involving tissue culturing of non-infectious samples, I.V. drug preparations and other pharmaceuticals that could have adverse health effects on patients and other techniques requiring a contamination-free atmosphere. Please note that this cabinet provides product protection only and offers no personnel protection from hazardous substances. The adequacy of a cabinet for user safety should be determined on-site by an industrial hygienist, safety officer or other qualified person. Remember that you, the owner and user, are ultimately responsible and that you use your cabinet at your own risk. We recommend that this manual, along with the factory test report, be kept near the cabinet for convenient reference by operators and qualified maintenance personnel. If you have any questions about the use or care of your new SterilSHIELD cabinet, please do not hesitate to contact our Customer Service Department at for assistance or us at bakerco@bakerco.com. Sincerely, Dennis Eagleson President, CEO The Baker Company, Inc. P.O. Drawer E, Sanford, Maine (207) FAX (207) Visit our website at - i -

3 TABLE OF CONTENTS I - FUNCTION AND DESCRIPTION OF THE STERILSHIELD...4 AIRFLOW INSIDE THE STERILSHIELD...4 CABINET PRESSURE AREAS...5 ACCESS TO THE WORK AREA...5 DESIGN DETAILS...5 Performance assurance...5 Motor/ blower capacity...5 Interior wall construction...5 Cable ports (Optional)...6 Electrical duplex (Optional)...6 Protective screen...6 All-metal plenums...6 Stainless steel work surface...6 Cabinet exterior panels...6 Tested HEPA filter...6 Viewscreen...6 Drain valve...6 Work area and pass through lighting...7 Drain Pan Cleanability...7 Air balance adjustments...7 Easy filter access...7 Telescoping stand (optional)...7 Intake Air Prefilter...7 UniPressure Preflow Plenum...8 SPECIFICATIONS...8 Weight...8 Electrical Specifications...8 Environmental Conditions...9 SYMBOLS AND TERMINOLOGY...10 II - PREPARING THE STERILSHIELD FOR USE...10 CHECKING THE CABINET UPON ARRIVAL...10 THE USES OF A STERILSHIELD CABINET...10 LOCATION WITHIN THE LABORATORY...10 Proper placement within the laboratory is essential...10 INSTALLING THE CABINET...11 EXHAUST INTO THE ROOM...11 FINAL CONNECTIONS AND TESTS...11 III - PROPER CABINET USE...12 START-UP PROCEDURE...13 LOADING MATERIALS AND EQUIPMENT...13 RECOMMENDED WORK TECHNIQUES...14 UNLOADING AND WIPE DOWN...14 REACTING TO SPILLS...14 DECONTAMINATION...14 CLEANING AND DISINFECTING STAINLESS STEEL...14 Simple Cleaning...14 Disinfection...14 USING ANCILLARY EQUIPMENT...15 ABOUT THE HEPA FILTERS...15 REMINDERS ii -

4 IV - ON-SITE CHECKS AND MAINTENANCE PROCEDURES...17 THE AIRFLOW BALANCE...17 FILTER LEAK TEST...18 Filter leak test procedures:...18 MAINTENANCE NOTES...18 Cleaning the Work Area...18 Replacing the HEPA Filter...18 Filter Change Steps...18 Cleaning the Prefilter...19 Replacing the Fluorescent Lamps...19 TROUBLESHOOTING...19 STERILSHIELD DISASSEMBLY INSTRUCTIONS...21 APPENDIX...23 WARRANTY...24 REPLACEMENT PARTS LIST...25 DIMENSIONAL DRAWING...26 WIRING DIAGRAM iii -

5 I - FUNCTION AND DESCRIPTION OF THE STERILSHIELD The SterilSHIELD is a product protection Isolator of original design. It features vertical laminar airflow and a closed front viewscreen. The unit is designed to protect only the product within from airborne contaminants. The SterilSHIELD does not offer personnel or environmental protection. The cabinet is vented directly into the laboratory. Airflow Inside the SterilSHIELD The SterilSHIELD cabinet features The Baker Company's laminar airflow design. (See Fig. 1) The stainless steel metal diffuser just below the supply HEPA filter creates a uniform airflow over the work zone. The air pressure is positive in the work zone to insure maximum product protection. Another feature of the Baker design is the pass-through chamber, which maximizes the cabinet's product protective capability. The pass-through chamber allows operators to introduce or remove items from the main work chamber while maintaining the internal sterile conditions. Both the pass-through chamber and the main working chamber operate at positive pressure to protect the product. The main chamber operates at a higher positive pressure than the pass through chamber. This insures that HEPA filtered air flows out from the main chamber. Contaminated room air cannot enter the main chamber thereby maintaining the internal conditions. The pass through chamber is equipped with both an external door to the outside and an inter-chamber door to the main chamber. These doors should not be opened at the same time except for cleaning. The front viewscreen has two gloveports equipped with gloves to provide a physical barrier between the technician and the product to insure maximum product protection. The front viewscreen is also hinged along the top and may be fully opened for cleaning, loading, or unloading. The Baker Company has also included ergonomic enhancements into the design of the SterilSHIELD for improved operator comfort and safety. A 10 degree sloped front feature has been incorporated into the design to improve ergonomics so the operator can use the cabinet properly without sacrificing comfort or safety. Room Air Intake Exhaust Air (-) Cabinet & Room Air (+) Cabinet & Room Air (+) HEPA Filtered Air [Pass- Thru (+) HEPA Filtered Air [Work Chamber] Fig. 1: Airflow Inside Cabinet (+) = Positive Pressure ( ) = Negative Pressure - 4 -

6 Cabinet Pressure Areas One of the many features that sets the SterilSHIELD unit apart from other cabinets is the interaction of its pressure areas. The main chamber operates at a higher positive pressure than the adjacent pass through chamber. This allows clean, HEPA filtered air to flow into the pass through chamber when the interchamber door is open to insure product protection. The SterilSHIELD is equipped with a motor blower that draws room air into a positive pressure plenum. The air then passes through a HEPA filter and a stainless steel diffuser. This provides unidirectional (laminar) airflow in the main and pass through chambers. All main and pass through chamber air is combined under the work surface where it is pulled, under negative pressure, through plenums in the rear and sides of the cabinet back into the motor-blower. Some of the air is re-circulated and some is vented into the laboratory. The SterilSHIELD provides better than Class 100 (ISO Class 5) air cleanliness conditions within the enclosure. Access to the Work Area For easy entry of apparatus into the work area, SterilSHIELD has a top hinged viewscreen. Its ¼-inch shatterproof polycarbonate viewscreen allows excellent visibility, and can be opened fully to permit placement of large items in the work area. While in operation the pass through can be used to bring items in and out of the cabinet. Design Details Performance assurance Meticulous care in manufacturing is followed by performance tests prior to shipment of your cabinet. In addition, a complete factory test report on the performance of your cabinet is included at the rear of this manual. Motor/ blower capacity A motor/blower's capacity is measured by its ability to provide a nearly constant volume of air as resistance increases because of filter loading. Verification by a simulated filter-loading test has established that your SterilSHIELD cabinet is capable of automatically handling a 30% increase in pressure drop across the filter without reducing total air delivery more than 10%. With the use of the manual speed control, a 117% increase in the pressure drop across the filter can be handled. This results in extended filter life, which relates to significant cost savings over the life of the cabinet. Interior wall construction The interior side and rear walls of your SterilSHIELD work area are constructed of 16 gauge type 304 stainless steel. The easy-to-clean 7/16-inch radiused (rounded) corners prevent buildup of contaminants and resist corrosion

7 Cable ports (Optional) A cable port is located in the cabinet s right side wall. It provides a way of introducing power and data cables, or siphoning tubes into the work area of the cabinet without having to go through the front viewscreen access opening. A plug is provided for each port, to be used when the port is not being used or the cabinet is being deconed. Electrical duplex (Optional) A GFI protected duplex outlet with weather-proof cover is located in the right side wall of the work chamber. This outlet is protected by a self-resetting circuit breaker to allow 5A maximum total to all outlets. Protective screen Located under the work surface at the bottom rear and sides of the return-air plenums, a protective screen, integral to the interior walls, is provided to prevent wipes and other paper materials from being drawn into the blower system. This feature helps to eliminate costly downtime for cleaning the blower and possibly the HEPA filter. The screen affords easy access to waste materials and should be kept clean at all times. All-metal plenums The plenums of your SterilSHIELD unit have been constructed entirely of metal in order to provide strength, durability, air-tightness and resistance to deterioration. Stainless steel work surface The work surface is constructed of corrosion-resistant 16-gauge type 304 stainless steel, with a #4 satin finish that diminishes light reflection. It has radius corners for easy cleaning. Cabinet exterior panels External panel construction is of 16-gauge cold-rolled steel protected by a smooth white baked enamel finish. Tested HEPA filter The filter in the SterilSHIELD is a scan-tested HEPA filter. It is 99.99% effective on particles of 0.3 micron in size. Each filter is scan-tested after installation to assure that there are no leaks >.01% of upstream concentration. Viewscreen The cabinet's ¼-inch shatterproof acrylic viewscreen may be fully opened for placement of large items in the work area, and should be fully closed for operation. Warning! For operator safety, the door should always be in the open and latched condition when access to interior workspace is required. Drain valve A 1/2 stainless steel ball valve in the SterilSHIELD unit is installed for safe and effective drainage of the drain pan

8 Work area and pass through lighting The lighting system produces at the work surface of the work area an average of 97 foot-candles of illumination and 48 foot-candles at the work surface of the pass through. The fluorescent light is externally mounted to minimize heat buildup. The unit uses one F482T12-CW-HO fluorescent bulb. Drain Pan Cleanability The drain pan is designed with 7/16 radius in all four bottom corners to facilitate cleaning. The worksurface supports have been designed to be removable for easy cleaning and to allow clear unobstructed access to the whole drain pan area during the cleaning procedure. Air balance adjustments Air balancing can be done by either of the following methods. However, only a technician with proper training and equipment should perform balancing. (See Section IV, "On-Site Checks and Maintenance Procedures.") A speed controller controls the down flow velocities. It automatically compensates for minor voltage differences. It can be manually adjusted to adjust for filter loading. Adjustable external dampers compensate for pressure resistance drop differences in the HEPA filter if it is changed at a later date and for cooling the cabinet. Easy filter access For convenience and ease of service, the HEPA filter is installed and removed through the front of the cabinet without entry into the work area. A qualified technician should only replace the filter. Telescoping stand (optional) The optional support stand provides the following range of heights: Knee Space Height Work Surface Height Overall Height Minimum Setting 28 1/8 30 1/8 92 3/8 Maximum Setting 36 5/8 38 5/ /8 If the stand is shipped disassembled, instructions on assembling and installing the stand are packed in the shipping box with the stand. The stand may also come equipped with an optional adjustable footrest. Intake Air Prefilter The intake air prefilter is located at the top left side of the cabinet. It lays over the room air intake opening for the blower and helps filter out large particulate before it can get to the HEPA filter. UniPressure Preflow Plenum The steel supply plenum in the SterilSHIELD provides the user with a number of desirable features - 7 -

9 which enhance safety and reliability while reducing down time during servicing: 1. This unique plenum design directs air across then through the HEPA filter, improving downflow uniformity. 2. The plenum serves as the filter sealing mechanism providing a continuous and evenly distributed clamping force around the complete perimeter of the filter. This approach eliminates potential filter frame damage as experienced with typical approaches that utilize point forces. This method assures a secure and sealed filter. 3. The gasket on both sides of the filter frame helps reduce the transmission of vibration through the unit from the motor blower assembly. 4. The motor blower assembly is mounted on a slide plate, which forms one wall of the plenum. This plate is removed by loosening one screw and disconnecting an electrical plug connector. 5. The supply plenum is raised and lowered by turning two threaded rods. These screw clamps exert a force on the plenum, which distributes the clamping force uniformly to the filter frame. Specifications Weight SterilSHIELD cabinet on standard tube stand: 760 pounds SterilSHIELD cabinet on standard tube stand: pounds Shipping weight: Add pounds to above weights. Electrical Specifications All electrical wiring should comply with the National Electrical Code and any applicable Local Electrical Codes at the site of installation. A single 115V AC, 20A, 60 Hz, 1 Phase dedicated circuit is required to power this unit. The unit is furnished with one 14' power cord with 20-amp plug, type NEMA 5-20P. Typical power for standard unit 8.0 Amps Optional Lift system (Intermittent Duty) 2.0 Amps Optional Receptacles 5.0 Amps Optional Air Pressure Monitor 0.4 Amps The power cord is the Main Disconnect device for the unit. The unit should be positioned in a manner that allows easy access to the power cord connection to the electrical utility. The cabinet is internally protected with a 250V, 20A Circuit breaker

10 The cabinet incorporates Baker s StediVOLT motor speed controller for the blower motor. This compensates for minor variations in incoming line voltage. The cabinet may be provided with an optional Electro/Hydraulic lift system rated at 115V, 2A. The cabinet may be provided with optional duplex receptacles. 115V, 5A total all outlets. The cabinet may be provided with an optional 24V DC power supply and cabinet pressure monitor. Environmental Conditions The cabinet is designed for use in the following conditions: Indoor use Altitudes up to 2000 meters Temperature range from 5 C to 40 C Maximum relative humidity 80% for temperatures up to 31 C decreasing linearly to 50% at 40 C Main Supply voltage 105 to 125 VAC Transient overvoltage according to Installation Category (OVERVOLTAGE CATEGORIES) II per UL 61010A-1, Annex J Pollution Degree 2-9 -

11 Symbols and Terminology Protective Earth: Any terminal intended for connection to external protective conductor for protection against electric shock in case of a fault.! General Caution: Refer to instruction manual for information regarding personnel and environment protection. II - PREPARING THE STERILSHIELD FOR USE Checking the Cabinet Upon Arrival Upon receipt of your new SterilSHIELD cabinet, first inspect the exterior of the crate, box and/or skid. If there is any visible damage, that fact should be noted on the receiving slip and immediately reported to the delivering carrier. Next, remove the packing material and inspect the cabinet itself. Remove the cabinet from the skid with a forklift or other available equipment. If any concealed damage is found it should be reported to the delivering carrier. A claim for restitution should be filed within 15 days. Due to the risk of mishandling by trucking companies, Baker has removed certain parts of the cabinet and has packed them separately. The Uses of a SterilSHIELD Cabinet The SterilSHIELD has been designed to provide a work area which protects the experiment from the environment and operator. The laminar flow cabinet is designed for work with non-hazardous materials. The SterilSHIELD does not offer any personnel or environmental protection. Biological, chemical, or other hazardous substances should never be used in this cabinet. CAUTIONS: * Hazardous materials should never be used in this cabinet. * Explosive or flammable substances should never be used in this cabinet. * If hazardous biological work is to be performed, a biological safety cabinet should be used. Do not use the SterilSHIELD for biological work. * If chemical, radiological or other non-microbiological hazards are present, do not perform this work in the SterilSHIELD. Call upon a suitably trained individual to advise other protective measures. Location Within the Laboratory The ideal location for any laminar flow cabinet is in a dead-end corner of the laboratory away from personnel traffic, vents, doors, windows. Proper placement within the laboratory is essential. The SterilSHIELD exhausts air into the laboratory instead of venting to the outside, it is important that there is adequate space between the top of the cabinet and the ceiling. A solid ceiling located too close to the exhaust opening will restrict the exhausting air. The necessary distance between exhaust opening and

12 ceiling is 4 inches. Consult with our Customer Service Department for the implications of this, and for alternatives. Installing the Cabinet Installation of this cabinet should be carried out in accordance with appropriate OSHA regulations and those of other regulatory agencies having jurisdiction. 1. Remove the protective container from around the cabinet. 2. Remove the strapping that holds the cabinet to the pallet. 3. Remove the tape that secures the work surfaces. 4. The small cardboard box taped to the work surface contains the drain valve. 5. The drain valve must be installed before the cabinet is either installed on the stand or placed on a counter top. 6. If ordered with the optional stand, refer to the stand installation and assembly instructions packed in the shipping box with the stand. NOTE: If you place your unit on the optional stand and you need to move the unit, we recommend you leave the unit on the stand. If you must take the unit off the stand to move it, disassemble the stand before moving it and reassemble at the new location. Exhaust into the room Never use the top of the cabinet or the work area for storage purposes, and never use flammable, explosive or toxic vapors or gases, or substances, which generate them, unless a qualified safety professional has evaluated the risk. The filter removes only particulates and not gases, thus causing the recirculation of these within the cabinet. Final Connections and Tests 1. A 20-amp power cord with a NEMA 5-20P plug is furnished with the SterilSHIELD. It should be plugged into an appropriate 115 Volt AC, 60 Hz, 20 Amp dedicated utility outlet. 2. Thoroughly clean the interior of the cabinet. 3. Press the blower switch. The blower should start. 4. Turn on the fluorescent light and make sure the bulb is lighted. The light will not go on unless the blower switch is on. 5. If the optional Hydraulic lift is installed verify proper operation. 6. If the optional Duplex receptacles are installed verify proper operation. 7. If the optional Air Pressure monitor is installed verify proper operation. 8. Although all units are carefully tested at the factory, it is advisable that certain other checks are made on-site by a qualified technician after installation. These include testing the filter for leaks and checking the air balance of the unit. A description of these tests can be found in Section IV, On-site Checks and Maintenance

13 9. It is also recommended that all personnel who will be using the cabinet study this Operator s Manual to learn how to use the cabinet most effectively. For additional start up and use procedures, please turn to Section III, Proper Cabinet Use. III - PROPER CABINET USE A product protection Isolator is a valuable supplement to good sterile technique, but is not a replacement for it. If the cabinet is not understood and operated correctly as defined in this manual it will not provide adequate protection for personnel or product. All activities that are to be performed in your cabinet should first be approved by a competent professional, such as an industrial hygienist or safety officer, to make sure that the cabinet is appropriate for the work it will be required to do. This person should monitor the cabinet and its operating personnel at regular intervals to see that it is being used correctly. In order to keep the interior workspace clean and free of particulates, all Baker laminar flow cabinets are designed for continuous operation. If the blower is turned off, the unit becomes contaminated with room air. Therefore it is recommended that the blower be left on at all times. UV Light (Not Available) Fluorescent Light Duplex Outlet (Optional) Blower Alarm Mute Switch (Optional)

14 Operator Controls The operator controls and indicators are arranged on the front panel of the unit in a single membrane switch assembly. (See Fig. 2) 1 Blower On/Off Switch This switch controls the power to the blower for the cabinet. When the switch is turned on, the yellow indicator light below the switch will light. 2 Fluorescent Light On/Off Switch This switch controls operation of the fluorescent light. The blower must be turned on for the fluorescent light to operate. The blue indicator light below the switch will light when the fluorescent light is turned on. 3 Duplex Outlet Switch (Optional) This switch controls the power to the duplex outlets. The blue indicator light below the switch will light when the outlets are powered. 4 Alarm Mute (Optional) When the Pressure monitor option is installed this switch will mute the alarm for 5 minutes. The red indicator below the switch will flash when an alarm exists. Start-up Procedure 1. If the unit has not been left running continuously, press the blower on/off switch. The yellow indicator light below the switch should be on. Make sure that you have cabinet airflow by listening for the sound of the blower. Check the readings on the mag gauges. They should be consistent with the last time the unit was on. 2. Turn on the fluorescent light. The blue indicator below the switch will should be on. 3. Check to determine that the drain valve is in the closed position or the drain coupling is capped. 4. Verify that work area and pass through grills are free from any obstructions. 5. Inspect gloves & sleeves for holes. 6. Latch viewscreen in the closed position. This cabinet should never be operated with the viewscreen open. 7. Allow the cabinet to run at least 3 minutes. 8. Wipe down the interior area of the cabinet with a surface disinfectant. Note: Some disinfectants, such as bleach or iodine, may corrode or stain the steel surfaces. If this happens, thoroughly clean the surfaces afterward with a detergent and rinse with sterile water to prevent corrosion. Loading Materials and Equipment 1. Place all materials and equipment needed in the cabinet via the pass through. Close the exterior door before opening the interior door. Make sure to disinfect before moving items from the pass through chamber to the work chamber. 2. Avoid blocking front and rear grills. 3. Arrange materials to minimize movement within the cabinet. 4. Leave the interior pass through door closed while working in the work chamber

15 Recommended Work Techniques 1. Perform all work on or over the solid work surface. Avoid working on the grills. 2. Keep the viewscreen closed and latched. 3. Limit opening of pass through doors when moving materials or equipment. Unloading and Wipe down 1. Close the interior pass through door before opening the exterior pass through door when unloading materials and equipment. 2. Wipe down the work and pass through chambers with disinfectant. Never use the cabinet to store supplies or laboratory equipment. 3. The viewscreen may be opened to facilitate cleaning or unloading. Do not attempt to utilize the provided workspace unless the latch is engaged, or if there is evidence of the springs are failing. 4. We recommend that the cabinet be left running continuously to ensure cleanliness. If the user elects to turn the cabinet off at the end of a work session, the viewscreen and outer pass through door should be closed completely and latched. Reacting to Spills Because use of hazardous materials is prohibited within the SterilSHIELD, spills should have no risk to the operator. Spills should be cleaned immediately to prevent cross contamination to the work and to avoid any damage to the stainless steel surfaces. Decontamination Because hazardous materials are prohibited within the SterilSHIELD, decontamination should not be required before maintenance activities. However, to protect your work, surfaces should be decontaminated before work begins. Cleaning and Disinfecting Stainless Steel Simple Cleaning Caution Do not use steel wool or steel pads when cleaning stainless steel. Dirt deposits on stainless steel (dust, dirt and finger marks) can easily be removed. Frequently, warm water, with or without detergent, is sufficient. If this does not remove the deposits, mild, non-abrasive household cleaners can be used with warm water and bristle brushes, sponges or clean cloths. Iron rust discoloration can be treated by rubbing the surface with a solution of 15% to 20% by volume of Nitric Acid and water and letting it stand for one to two minutes to loosen the rust. Disinfection The purpose of disinfection is to destroy particular organisms that could pose a potential hazard to humans or compromise the integrity of the experiment. It is important to use a suitable disinfectant in the

16 concentration appropriate to the organism being killed. Standard disinfectants include: Hypochloride (chlorine bleach), Iodophor-Detergent, Ethanol, Phenol and Alcohol. Important Disinfection and cleaning should always be followed by rinsing in clean hot water and wiping the surface completely dry. Disinfect the work surface before and after every procedure. 1. Disinfect surfaces of all equipment used. 2. Remove all items from the inside of the cabinet. 3. Place all items that may have come in contact with the agent(s), such as used pipettes, in a plastic bag or other suitable container. 4. Disinfect the entire inside surface of the cabinet. For additional information on cleaning and disinfecting stainless steel, please refer to: Decontamination, Sterilization, Disinfection, and Antisepsis, Vesley, Donald and Lauer, James L., Laboratory Safety Principles and Practices, Second Edition, 1995, Fleming, D.O., Richardson, J.H., Tulis, J.J. and Vesley, D., editors, ASM Press, Washington, D.C., pp ; and Biosafety Reference Manual, Second Edition, 1995, Heinsohn, P.A., Jacobs, R.R. and Concoby, B.A., editors, AIHA Publications, pp Using Ancillary Equipment The rule to keep in mind is that the more equipment placed in the cabinet, the greater will be the air turbulence it causes. The turbulence resulting from equipment and materials can disrupt the designed airflow and reduce the effectiveness of the cabinet. About the HEPA Filters The High Efficiency Particulate Air (HEPA) filter is one of the essential components of a clean air cabinet. It is the shield, which stands between the product and the environment. The HEPA filter consists of a continuous sheet of glass fibers pleated and mounted in a rigid frame. It is very delicate and the filter media should never be touched. Proven efficiency in all HEPA filters used in Baker cabinets are 99.99% for particles 0.3 microns in diameter. The 0.3 micron particle is used as the basis for filter definition because theoretical studies have shown that filtration efficiency should be at a minimum for particles of this diameter, with efficiency increasing for particles either larger or smaller. Experiments with various viruses and microbial agents have proven the effectiveness of HEPA filters. (Contact The Baker Company for more information). It must be pointed out that the HEPA filter is not intended to filter gasses or vapors, nor are they 100% efficient on particulates. Consider the filters inefficiency when performing a risk assessment. Since this cabinet is partially recirculating, there will be gaseous buildup to the point of equilibrium if these agents are used. The life of a filter is determined by the cabinet/ motor/blower system design and how it is used and how often. Under normal laboratory conditions, you can expect at least five years of use. However, misuse or a heavy dust load within the cabinet will shorten any filter's lifetime. Bunsen burners and misuse of chemicals will also shorten the useful life

17 Reminders After many years of seeing our equipment used and misused, we have made up this list of suggestions: Store equipment and supplies outside of the cabinet. Always leave the blower on. Keep the viewscreen closed. Avoid using an open flame within the cabinet unless it has been specifically approved by a safety professional. Always keep the air grilles clear and unobstructed. Toxic, explosive or flammable substances should not be used in the SterilSHIELD. The control system will adjust automatically for small changes in the load on the blower. A qualified technician should only make adjustments for larger changes. Don't change the cabinet or blower speed unless the change is required by a decrease in measured air velocity. Always close the drain valve after each use. If the operators are well trained and use good common sense when operating your cabinet, you should have very few problems

18 IV - ON-SITE CHECKS AND MAINTENANCE PROCEDURES We recommend that the following checks be performed before initial use, after relocation, and after each filter change. They should also be carried out at regular intervals, usually six months or one year, as specified by an industrial hygienist, safety officer or other qualified person. The tests described below meet recommended minimum requirements and only experienced technicians using proper procedures and instruments must perform them. Our representatives can tell you about other tests, which you may consider desirable. As reported earlier in this manual, each individual cabinet made by The Baker Company is carefully tested before it leaves the factory. Your copy of the test report, which you will find at the back of this manual, gives the factory test results for your own SterilSHIELD cabinet. Use it as your record of the original testing, and as your guide to testing in the future. To gain many years of satisfactory service, please be sure that your maintenance personnel come as close as possible to duplicating these original test figures. Your test procedures should be identical to ours so that achieving similar test results and comparison of data will be possible. Please correspond directly with us to request detailed procedures for your particular cabinet model. The Airflow Balance Warning! This procedure should be performed by qualified technicians only. The airflow balance, which is set at the factory, provides your cabinet with the proper air volume, velocity and chamber pressures. In order to duplicate as closely as possible the airflow characteristics described in the original factory test report, please follow these steps: 1. Close the front view screen and both pass through doors. 2. Perform downflow velocity test per manufacturer instructions. Specific details are in the factory test report located in the operators manual. NOTE: The IV bar needs to be removed while performing downflow readings. 3. Record the positive pressure readings for each chamber. 4. Compare your results with those originally recorded at the factory. 5. Make adjustments to the blower speed controller and the dampers as necessary. The speed controller is located inside the electrical panel. (Located on the upper left of the cabinet). Turn the screw clockwise/ counterclockwise to increase/decrease flow. The dampers are located on top of the cabinet. Slide the exhaust damper (on right hand side) open/closed to increase/ decrease flow. NOTE: As the HEPA filters load up with particulates, airflow will be maintained automatically, at least until the filter resistance increases 50% or more. When airflow eventually diminishes, you will have to increase the blower speed in order to maintain the original volume of recirculating air. There is a speed control located in the electrical panel. Turn it clockwise until you have the desired airflow. If the airflow cannot be maintained, it will be necessary to replace the HEPA filters. (See "Procedure for HEPA Filter Replacement" later in this section)

19 If the airflow cannot be maintained, it will be necessary to replace the HEPA filters. (See "Procedure for HEPA Filter Replacement" later in this section). Filter Leak Test The filters in your cabinet were tested at our factory before shipment to ensure that the media, gasket, and frame were not exceeding IEST-RP-CC034.1 for HEPA and ULPA filter leak tests. Since filters may become damaged in transport, we recommend that the filters be re-tested by qualified technicians before the cabinet is used. The filters should also be leak tested at prescribed intervals as specified by an industrial hygienist, safety officer or other qualified person. Filter leak test procedures: 1. Open front hinged view screen. 2. Remove IV bar, work and pass through down flow diffusers. 3. Close and seal with tape the exhaust damper located on the top right side of the cabinet. Make sure to mark the dampers position before closing it. 4. Perform the filter leak test per IEST-RP-CC The upstream DOP sampling port is on the top center of the cabinet. 5. Repair leaks as required per IEST-RP-CC Reinstall diffusers and IV bar and close the viewscreen. Maintenance Notes Cleaning the Work Area Spills that fall through the perforated grilles can be removed through the drain valve after proper treatment. To wash the drain pan under the work surface, lift up and surface decontaminate the solid work surface, the perforated grille, and remove the work surface supports. This provides unobstructed access to the drain pan for easy cleaning. Make sure you close the drain valve when you are finished cleaning the drain pan. Replacing the HEPA Filter If your periodic checks of total airflow show a drop of ten percent or more from the original settings, the filters may be loading with particulates. As explained earlier in this section, the blower speed can be manually increased to compensate for filter loading. However, when the airflow can no longer be maintained or when the filters are damaged, they need to be replaced. HEPA filters are very easily damaged, and you will want to use great care in handling so as to avoid injury to the filter media and gasket surfaces. Inspect the filters carefully before and after installation. A broken or damaged filter is worthless. Filter Change Steps 1. Close and latch the viewscreen. 2. Unplug the cabinet

20 3. Remove the hardware that holds the electrical panel closed and hinge it open. Disconnect the tygon tubing from the back of the magnehelic gauges (Label the tubes for correct reconnection). Disconnect the electrical cord. Mark the position of the adjustment screws on the panel hinges then loosen the screws and lift off the electrical panel. 4. Remove the hardware holding the right side dress panel and remove the panel. 5. Remove the inner closure panel by removing its hardware. Be careful not to damage the sealing washers or the gasket on the panel. The filter is now exposed. 6. Loosen the ½ - 13-plenum clamp bolts, located on each side of the supply plenum, approximately 3 ½ revolutions or until the supply plenum is raised about ¼ inch above the filter. 7. Loosen the filter gasket seal from the metal plenum. A putty knife works fine to break the gasket seal if necessary. Align the supply filter with the notches in the frame of the plenum and remove the filter by sliding it towards you. 8. Take the new filter and align it with the notches in the frame of the plenum and sliding it back against stops in rear. Make sure that the gasket remains attached on both sides of the filter. 9. The plenum clamps can now be tightened, alternately two to three revolutions until the gasket is compressed to approximately 3 / 16 inch. 10. Reinstall the front seal panel by first tightening each ¼ - 20 hex cap screw until the bolt seal washer is engaged with the panel. Next turn each bolt 1¼ to 2½ revolutions until the panel gasket is evenly compressed to 1 / 8 - inch thickness. Do not overtighten. (Do not use a power drive socket for this procedure). Cleaning the Prefilter The prefilter should be inspected periodically for particulate loading. The prefilter lays on top of the room air intake and can be removed for cleaning. Use a vacuum to clean the particulate off the perfilter then reinstall it over the room air intake. Replacing the Fluorescent Lamps Open the viewscreen. Remove screws securing the clear light diffuser and slide the assembly towards front of unit. Slide bulb to compress socket and remove. Install in reverse order. Troubleshooting Here are some suggestions based on our experience with the use and misuse of safety cabinets. When there is low airflow within the work area - 1. Check the incoming line voltage. Low voltage may cause the blower to operate at a slower-thandesigned speed. Although this should be corrected in the building's electrical system, you may be able to compensate by adjusting the blower speed control clockwise until proper velocity is reached. The control is located in the electrical panel. 2. Check the filter for loading or damage and replace, if necessary. 3. Check the position of the exhaust damper. If there is no air flow within the work area - 1. Check to make sure the blower switch is turned on. The yellow indicator below the button should be lighted

21 2. Check to make sure the unit is plugged into a dedicated electrical outlet (grounded 20 amp, 115 Volt AC, 60 Hz). 3. Check to make sure the wiring connections inside the electrical panel are pushed together properly. 4. Check to see if the lights are working. If they are, then turn the blower switch off and let the cabinet set for ten minutes. This allows the motor time to reset itself, if it has been overheated. Note: If the motor restarts and there is a whining sound, the motor bearings may be causing the motor to overheat. 5. If these solutions do not correct the problem, or if the blower failed to start after the rest period, then the speed control, blower motor, or capacitor is defective. A qualified electrician, using the wiring diagram in the Appendix of this manual, can by-pass the speed control to determine if it is defective. When there is uneven fan operation, or noise from the motor/blower assembly - 1. Open the electrical panel and check the speed controller. Also check the multi-pin connectors at each end to be sure they are securely engaged. 2. Check the blower wheel for contact with the blower housing. 3. Check the blower for loose objects. If the fluorescent light does not work - 1. The blower switch should be turned on, and the yellow indicator below the switch should be lit. 2. Check to see if the lamp is blown or not engaged properly. 3. Open the electrical panel and check the multi-pin connectors at each end to be sure they are securely engaged. If the lamp flickers and can be corrected by vigorous rubbing of the bulb, there is probably an improper ground. The wiring can be traced to the source of a break. If the cabinet interior heats up above normal levels - 1. Check the room air intake prefilter. If it is excessively loaded with particulate then air from the room is not getting in to cool the cabinet. Clean the prefilter. 2. Check the position of the exhaust damper. If it is closed more than it should be then open it up some. This will allow some of the warm air exit and also allow cooler room air into the cabinet. NOTE: Changing the dampers position will affect downflow and positive pressure levels in the cabinet. FOR MORE INFORMATION For a complete listing of articles, papers and reports related to containment, clean air products and safety, contact The Baker Company for our complete Bibliography or visit our website at

22 SterilSHIELD DISASSEMBLY INSTRUCTIONS TOOLS REQUIRED: (1) 3/8" Spin Wrench (2) 3/8" Socket and Drill (3) 3/4" Wrench and (4) 1/4" Spin Wrench (5) Screw Driver DISASSEMBLY: Electrical panel, right side dress panel and front access panel: 1. Close and latch the viewscreen. 2. Unplug the cabinet. 3. Remove the hardware that holds the dress panel closed and open it. Disconnect the tygon tubing from the back of the magnehelic gauges. Make sure to note which tube goes to which gauge. Disconnect the electrical cords. Lift dress panel up and off the tabbed hinge. 4. Using a 3/8" drive socket and drill, remove ten retaining bolts from the front access panel. Remove the center top bolt last. Be careful not to damage the seal washers or the gasket on the panel. The blower, supply plenum and filter are now exposed. Blower and filter: 5. Blower/motor removal - remove one bolt and clip at the forward edge of the supply plenum. 6. Disconnect the motor leads by pulling apart at each connection. 7. Remove the blower by sliding it out the front. 8. Loosen the ½ - 13-plenum clamp bolts, located on each side of the supply plenum, approximately 3 ½ revolutions or until the supply plenum is raised about ¼ inch above the filter. 9. Loosen the filter gasket seal from the metal plenum. A putty knife works fine to break the gasket seal if necessary. Align the supply filter with the notches in the frame of the plenum and remove the filter by sliding it towards you. Viewscreen: 10. Unlatch and open the viewscreen. 11. Disconnect the gas cylinders that hold up the viewscreen and lower the viewscreen back down. 12. Relatch the viewscreen. 13. Remove the two ¼ hinge pins on the viewscreen, unlatch and remove viewscreen. 14. Lift out the front perforated grill at the worksurface. 15. Lift out the worksurface pan and supports. Side access panel: 16. Using a 3/8" drive socket and drill, remove seven retaining bolts from the right side access panel. Remove the top bolt last. Be careful not to damage the washers or the gasket on the panel. 17. Repeat #17 on left side access panel. Pass through wall: 18. Remove #8-32 hardware along rear wall of cabinet. 19. Remove hardware and clip at bottom front of pass through wall. 20. Remove #8 truss head screw at to front of pass through wall and remove wall. 21. Remove #8 hardware in the upper removable pass through wall filler and remove filler piece. REASSEMBLY: Use the disassembly instructions in reverse order. (See Notes on next page)

23 NOTES: Replace the filter by simply aligning it with the notches in the frame of the supply plenum and exhaust filter brackets, sliding it back against stops in the rear, ensuring that the gasket remains attached on both sides. The supply plenum clamps should be tightened, alternately two or three revolutions until the filter gasket is compressed approximately 1/8 inch. Reinstall the front seal panel by first tightening each ¼-20 hex cap screw until the bolt seal washer is engaged with the panel. Next turn each bolt 1 ¼ to 2 ½ revolutions until the panel gasket is evenly compressed to 1/8 inch thickness. Do not over tighten. (Do not use a power drive socket for this procedure)

24 Appendix

25 Warranty The Baker Company, Inc. expressly represents and warrants all goods (a) to be a specified (and described) in The Baker Company catalogues and literature, and (b) to be free under normal use, service and testing (all as described in The Baker Company, Inc. catalogues and literature) from defects in material and workmanship from a period of thirty six months from the invoice date. The exclusive remedy for any breach or violation of this warranty is as follows: The Baker Company, Inc. will F.O.B. Sanford, Maine furnish without charge repairs to or replacement parts or equipment which proved defective in material or workmanship. No claim may be made for any incidental or consequential damages. THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTIBILITY OR FITNESS FOR A PARTICULAR PURPOSE UNLESS OTHERWISE AGREED IN WRITING SIGNED BY THE BAKER COMPANY. (THE BAKER COMPANY SHALL NOT BE RESPONSIBLE FOR ANY IMPROPER USE, INSTALLATION, SERVICE OR TESTING OF GOODS.)

26 Replacement Parts List Electrical Items Baker Part Number Fluorescent Lamp Lamp Holder Plunger End Lamp Holder Fixed End Ballast Relay / Heat Sink Speed Control Capacitor Capacitor Boot Transformer Terminal Block Membrane Switch Circuit Board Membrane Switch Touch Pad overlay / Label Mechanical Items Baker Part Number Mag. Guage Gas Cylinder Pass Through Door, Sliding 347A008 Pass Through Door, Hinged 347A306 Viewscreen (Lexan only) 347M309 Main Viewscreen 347A312 Sleeve with Glove, Ambidextrous, Neoprene Connector, Gloveport Sleeve HEPA Filter, 20 x 60 x Blower Plate Assembly Inlet Prefilter, Scottfoam 333A M

27 CHECKED INLET PREFILTER PASS - THROUGH DOOR: 18 GA. 304 SS 1/4" THICK LEXAN. 3. FILTER, MOTOR, BLOWER AND ALL ELECTRICAL COMPONENTS ARE FRONT ACCESSIBLE. ±1/32 REV FRACTIONS 87 3/4 MIN ELECTRICAL ACCESS PANEL MAG GAUGE FOR WORK CHAMBER MAG GAUGE FOR PASS - THROUGH CABINET CONTROLS PASS - THROUGH DOOR INLET DAMPER 65 3/4 A A D.O.P. PORT EXHAUST DAMPER VIEWSCREEN LATCH VIEWSCREEN LATCH /8 TO BOTTOM OF DRAIN PAN 28 1/8 MIN HEIGHT TO BOTTOM OF UNIT (INCLUDES 2 1/2 OF LEG LEVELER ADJUSTMENT) NOTES: 1. ELECTRICAL REQUIREMENTS: 115 vac, 60 hz, SINGLE PHASE, 20 AMP POWER CORD 14 FT. WITH 20 AMP PLUG. 2. CONSTRUCTION: WORKSURFACE / WORK AREA: 16 GA. 18 GA., 304 SS, #4 FINISH. HINGE VIEWSCREEN IMPORTANT NOTE: THIS IS A PRODUCT PROTECTION CABINET AND OFFERS NO CONTAINMENT FLOURESCENT LIGHT DIVIDER BETWEEN WORK CHAMBER AND PASS THROUGH GLOVEPORT WITH TWO-PIECE GLOVE FLAT WORKSURFACE 51 3/8 WIDE IN WORK CHAMBER 18 9/16 WIDE IN PASS THROUGH BALL VALVE DRAIN 1/2" NPT 20 5/8 WORKSURFACE SECTION A-A 20 1/2 SUPPLY HEPAFILTER 23 3/8 SUPPLY DIFUSER 10 POSITION IV BAR VERTICAL SLIDING DOOR 26 1/2 SterilSHIELD (5FT) 1 OF 1 A DRAWN DECIMALS MACHINED SHEET METAL ANGULAR.X ±.02 ±.06 ±0'-30 (.5 ).XX ±.01 ±.03.XXX ±.005 ±.010 THIRD ANGLE FINISH: 125 DATE DATE 56 7/8 TO WORKSURFACE ACCESS PANELS, BLOWER SECTION: 16 GA. & 18 GA., CRS WITH BAKED ENAMEL FINISH, BAKER PERMA WHITE. VIEWSCREEN FRAME: 16 GA. 304 SS 1/4" THICK LEXAN. A ORIGINAL RELEASE D - NO 4 PROJECTION SCALE SQUARE FOOTAGE SHEET TITLE SIZE MATERIAL DWG NO D C B A D C B A 347D700G.doc Dimensional Drawing 96 1/4 MAX 36 5/8 MAX 1 1/8 2 3/4-26 -

28 Wiring Diagram