Operator s Manual. Rev F 1/2/ O Neil Rd, Macedon NY Telephone: (315) Fax: (315)

Transcription

1 Rev F 1/2/ O Neil Rd, Macedon NY Telephone: (315) Fax: (315)

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3 Table of Contents Introduction... 5 Warranty... 5 Filter Bags... 5 Operating Environment... 5 Contact Information... 6 Instrument Description... 7 Safety Precautions... 8 Instrument Installation and First Run... 9 Fat Extraction Calculations Fat Extraction Support Items Sample Preparation Fat Extraction step-by-step procedure using the ANKOM XT Periodic Maintenance Troubleshooting & Replacement Parts Diagnostics Appendix A Electrical Diagram (pg. 1 of 3)... 33

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5 Introduction ANKOM Technology designs, manufactures, and markets instruments and support products used by analytical laboratories around the world in the environmental, agricultural, biomass, and food industries. ANKOM Technology can provide you with products for determining or monitoring dietary fiber, detergent fiber, fat, digestibility, microbial fermentation (anaerobic or aerobic) and more. Committed to Total Customer Satisfaction, ANKOM designs every product based on a thorough assessment of customer needs. Congratulations on your purchase of the ANKOM XT15 Extraction System. We are confident that this product will effectively serve your needs. The ANKOM XT15 Extraction System was designed to automate the process of fat extraction. This reduces technician variation, increasing precision. By carefully following the operating instructions in this manual you will understand the details of sample and filter bag handling as well as the instrument controls, helping you to achieve the best possible results. Please review the entire contents of this manual before you begin operating this product. Warranty ANKOM Technology warrants the ANKOM XT15 Extraction System against any defects due to faulty workmanship or material for one year after the original date of purchase. This warranty does not include damage to the instrument resulting from neglect or misuse. During the warranty period, should any failure result from defects in workmanship or materials ANKOM Technology will, at its discretion, repair or replace the instrument free of charge. Extended warranties are available upon request. Filter Bags Use only ANKOM Technology filter bags (part # XT4) in your ANKOM XT15 Extraction System. Use of any other filter mechanism will void the warranty. Filter bags can be purchased from ANKOM Technology or from your local authorized ANKOM distributor. Operating Environment Your ANKOM XT15 Extraction System is designed to operate within the following environments: Ambient Temperature Range: C Power: 100V 120V ~ 50/60Hz 10A 220V 240V ~ 50/60Hz 5A Rev F 1/2/18 pg. 5

6 Contact Information At ANKOM Technology we are committed to your total satisfaction and therefore always available to help you get the most from your ANKOM products. We are also very interested in any comments or suggestions you may have to help us improve. For any questions or suggestions regarding your instrument, please contact us at: Telephone: (315) Fax: (315) pg. 6 Rev F 1/2/18

7 Instrument Description General Description The ANKOM XT15 Extraction System is designed to extract Crude (Free) Fat using conventional solvents, typically petroleum ether. The compounds extracted are primarily triacylglycerols together with a small quantity of related lipids, traditionally termed Crude Fat. The analysis is achieved by measuring the loss of mass due to the extraction of fat/oil from the sample encapsulated in a filter bag. The quantitative isolation of the sample is accomplished by surrounding the sample in a sealed filter bag with a filtering capacity in the 2 to 3 micron range. The filter bag has sufficient porosity to permit rapid solvent passage and is composed of polymeric material that is resistant to the higher temperatures and solvents used in the instrument. The quantitative isolation achieved by the filter bag permits the ANKOM XT15 Extraction System to automatically process samples in batches of up to 15. With the solvent reservoir filled, simply place filter bags with sample in the Extraction Vessel, lock the vessel in place, select the extraction time and temperature, and press Start. The instrument will automatically fill the Extraction Vessel with solvent, extract the fat from the sample, and recycle the solvent. Below is a detailed view of the ANKOM XT15 Extraction System. Power Switch (not visible) Display Keypad Solvent Fill Port Safety Valve Handle Main Handle Pressure Gauge Solvent Sight Level Extraction Vessel Reflux Water Flow Control The Reflux Water Flow Control measures and controls the flow of water through the condenser to rapidly cool the solvent vapors during the extraction process. The proper water flow is set at the manufacturer. Contact ANKOM at service@ankom.com if the flow measurement is incorrect. Rev F 1/2/18 pg. 7

8 Safety Precautions Flammable Hazard: Static electricity is to be avoided as it is a spark hazard. Hot Surfaces: Do not touch the extraction vessel during operation. The surface can exceed 70 C (158 F). Failure to observe this caution may result in injury. Hazardous Voltages: Do not operate the instrument with the electrical compartment open. Hazardous voltages are present during operation. Failure to observe this caution may result in electrical shock or electrocution. Hazardous Materials: The ANKOM XT15 Extraction System comes equipped with a charcoal filter which traps any residual vapor in the vent system. By properly maintaining the charcoal filter, volatiles will not escape the vent system. We recommend replacement of the filter every 3 months or after 100 extraction runs. Trace amounts of vapor may also be present when samples are removed. It is recommended that the ANKOM XT15 Extraction System be placed in a well ventilated environment, away from any heat or ignition sources. Organic solvents may be flammable. Follow safe laboratory practices according to both local and federal regulations when installing this instrument and when handling any organic solvents. WARNING: Attempts to override safety features or to use this instrument in a manner not specified by ANKOM Technology voids the warranty and may result in serious injury or even death. This system is designed to meet and/or exceed the applicable standards of CE, CSA, NRTL and OSHA. Please review the entire contents of this manual before you begin operating this instrument. pg. 8 Rev F 1/2/18

9 Instrument Installation and First Run Site Requirements To install and operate the ANKOM XT15 Extraction System you will need the following: Adjustable wrench Chilled or tap water supply not to exceed 23 C Adequate power (see Operating Environment section) Drain Instrument Installation and First Run Procedure To install the ANKOM XT15 Extraction System and execute the first run, follow the procedure detailed below. 1. Remove the instrument from the shipping container and place it on a smooth level surface in an area that is free of dust and excessive moisture, and is well ventilated (away from any heat or ignition sources). Because the ANKOM XT15 Extraction System is a closed system, there is very limited exposure to solvents. When used in conjunction with a charcoal filter on the vent line the instrument produces less than 1ml of volatiles. It is the user s responsibility to determine whether venting of this instrument requires placement in a hood. Please follow both local and federal regulations when installing this instrument. 2. Connect the tubes as shown in the picture below, ensuring that there are no kinks or bends. Vent Flask Connector Tube to connect to the Charcoal Filter Water Supply (Top Tube) Drain (Bottom Tube) Vent Flask You can connect the Water Supply and Drain tubes to a chiller to reduce the amount of waste water used by the lab. 3. Connect the vent flask to the Vent Flask Connector on the instrument and run the tubing to the inline charcoal filter. Periodically check the vent line to verify that it is free from any blockage or kinks. Generally, there should not be any significant solvent in the vent flask. Any solvent visible in the flask may indicate a problem with the water supply to the instrument. Change the Charcoal Filter every 3 months or after 100 extraction runs. 4. Plug the power cord into a grounded receptacle. Rev F 1/2/18 pg. 9

10 5. To complete the installation, run the instrument (without samples) according to the procedure below. 5.1 Turn the instrument power ON. The Display will briefly show the software version number followed by the screen to the right. PET Ether v <ENTER> 5.2 Select the solvent you want to use. The factory default solvent shown on the Display is "Pet Ether." However, the instrument computer will store in memory the last solvent selection made and use it as the default solvent shown on the Display screen every time the instrument is ready for a new run Press the Down Arrow (v) key on the Keypad until the desired solvent is shown on the Display Fill the solvent reservoir as needed Unscrew the Fill Cap Pour solvent into the Fill Port up to the GREEN level mark on the Solvent Sight Level. (About 500 ml of solvent is needed to reach the GREEN level mark.) Do not add solvent up to the top of the Solvent Sight Level tube or it will start flowing into the Vent Flask. If using Diethyl Ether or a solvent mixture that includes Diethyl Ether (such as the Mojonnier Mixture), you will need the O-ring Kit for High Penetration Solvents (part # X95) to replace the standard O-rings on the top of the Extraction Vessel and the Valve Kit for High Penetration Solvents (part # X55). Warning: Do NOT use Acetone in this instrument because it will cause damage to the valves Press ENTER on the Keypad to complete the solvent selection. The Display should now show the screen to the right. Remove/Insert Samples <ENTER> 5.3 Check that the Extraction Vessel is properly installed in the instrument Press down the Safety Valve Handle to release the Safety Valve Pin and rotate the Main Handle upward until it stops. pg. 10 Rev F 1/2/18

11 5.3.2 Use the Extraction Vessel Handle to pull the Extraction Vessel out Slide the Extraction Vessel toward you far enough to verify the white PTFE Insert is in the Extraction Vessel If you removed the PTFE Insert, put it back into the Extraction Vessel, aligning the PTFE Insert slot with the Extraction Vessel pin. PTFE Insert O-ring Extraction Vessel O-ring PTFE Insert slot and Extraction Vessel pin The PTFE Insert O-ring and the Extraction Vessel O-ring must be in place before running the instrument. The PTFE Insert slot must be aligned with the Extraction Vessel pin before running the instrument Slide the Extraction Vessel into the instrument ensuring the Extraction Vessel O-ring stays in place Pull the Main Handle downward, closing and locking the Extraction Vessel in place. You will hear a click noise when the Safety Valve Pin locks in place. Before running the instrument, verify that the Extraction Vessel is locked in place by attempting to rotate the Main Handle upward. If it is not locked, repeat step #5.3.6 above Press ENTER on the Keypad. The Display should now show the screen to the right. (The time shown will be the last time entered.) Select time 60 (^v)... <ENTER> Rev F 1/2/18 pg. 11

12 5.4 Set the Extraction Time Using the arrow keys on the Keypad, set the Extraction time to 20 minutes for the initial run. (The recommended time is 60 minutes.) Press ENTER on the Keypad to set the Time. The Display should now show the screen to the right. (The temperature shown will be the last temperature entered.) 5.5 Set the Extraction Temperature Using the arrow keys on the Keypad, select the desired Extraction Temperature. (The recommended maximum temperature is 90 C.) Press ENTER on the Keypad to set the Temperature. The Display should now show the screen to the right. 5.6 Verify that the water supply is on. 5.7 Run the instrument. Press START on the Keypad. The instrument will run through its automatic operation, showing on the Display a time countdown along with process information. The first operation is to fill the vessel For future reference, record the countdown time on the screen when the vessel is full. The Heat light will turn on when the vessel is full. Select temp 90 (^v)... <ENTER> Close Vessel Water On <START> For future reference, record the countdown time on the screen when the heat reaches 90 C After the instrument reaches 90 C, record the reflux water flow in the flow controller. The value should be between 6 and 7 GPH (4 to 6 GPH if using a chiller). If necessary, turn the front knob on the controller counterclockwise to increase the value, and clockwise to decrease the value. The reflux process runs on 90 second cycles. The reflux controller must be read and adjusted during the first 1 minute of the cycle Verify that the XT15 runs through the drain sequence with no errors. When the instrument has completed its automatic operation, the Display will show the screen to the right. PET Ether v <ENTER> At this time, the only light shining on the Display should be the green power light. pg. 12 Rev F 1/2/18

13 Fat Extraction Calculations Crude Fat Calculation Crude Fat contained within a food or feed sample can be calculated using the following formula: % Crude Fat = 100 x (W2 W3) W1 Where: W1 = Original weight of sample W2 = Weight of pre-extraction dried sample and filter bag W3 = Weight of dried sample and filter bag after extraction For Liquid Samples, W2 and W3 in the equation above will include the weight of Diatomaceous Earth (DE). For Meat Samples with > 15% fat and plant samples with > 20% fat: % Crude Fat = 100 x ((W5 W4) W3) Where: W4 W5 Total Fat Calculation = = W1 Weight of the weigh tin Weight of Pre-dried Sample + Filter Bag + Weigh Tin If hydrolysis is done prior to fat extraction, then Total Fat contained within a food or feed sample can be calculated using the following formula: % Total Fat = 100 x (W2 (W3 + (C1 C2))) W1 Where: W1 = Original weight of sample W2 = Weight of dried sample, filter bag, and Diatomaceous Earth (DE) after hydrolysis W3 = Weight of dry extracted sample, filter bag, and DE C1 = Blank filter bag dry weight after hydrolysis C2 = Blank filter bag weight after extraction Fat Extraction Support Items The Hydrolysis procedure can be found in the ANKOM HCl Operator's Manual. The following support items are needed to perform the fat extraction procedure: Item Recommended Product Electronic Balance with four-place readout ANKOM #TB Balance Hardware ANKOM #TBS Balance Software Filter Bags ANKOM #XT4 Bag Holder (used for adding sample to an empty filter bag) ANKOM #101.2 Heat Sealer for sealing the filter bags ANKOM #HS (120V), #HSi (220V) Solvent Resistant Marker ANKOM #F08 Desiccant Pouch or Weigh Tin Desiccator for drying ANKOM #X45, #X49 Diatomaceous Earth (DE) - used with liquid samples ANKOM #DE1, DE2 Dryer (capable of temperatures from C) - optional ANKOM #RD (120V), #RDI (220V) Oven for drying (capable of maintaining 102 C±2 ) Ventilated oven trays to allow for sufficient oven air flow Weigh Tins (used with samples of > 20% fat) Sample Spoon Rev F 1/2/18 pg. 13

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15 Sample Preparation Sample Preparation Procedure for Crude Fat for LOW fat samples To prepare meat samples with 15% fat by weight or plant samples with 20% fat by weight for fat extraction, follow the procedure detailed below. 1. Using a Solvent Resistant Marker, number all of the empty filter bags you will use during the fat extraction procedure. Although it is not necessary in the determination of fat, it is recommended that you weigh and record the weights of all the filter bags prior to filling them in order to calculate the moisture value for the samples. % Moisture = 100 x ((Filter Bag Weight + Sample Weight) - Weight after drying) Sample Weight Measuring moisture can also help verify the operation of your drying oven. 2. Place an empty filter bag in the Bag Holder in an open position. 3. Tare the weight of the empty filter bag and the holder together. 4. Add 1.5 2g of sample to the filter bag. Keep all particles away from the sealing area of the filter bag. 5. Record the weight of the sample (W 1). 6. Set the Heat Sealer dial to 6. (The setting may vary from sealer to sealer.) 7. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 3 seconds after the red sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of the filter bag (as shown to the right). If the seal is not strong, re-seal the bag. 8. Repeat steps 1 7 for all filter bags that will be used in the ANKOM XT15 Extraction System. (Up to 15 bags can be processed during one procedure.) 9. Dry all samples at 102 C±2 for 3 hours to remove moisture prior to the extraction. 10. Remove the samples from the oven and place them in a Desiccant Pouch. 11. Allow the samples to cool to room temperature. This should take about minutes. 12. Re-weigh each bag (W 2) immediately after removing from the Desiccant Pouch. The samples are now ready for the fat extraction procedure. Seal Rev F 1/2/18 pg. 15

16 Sample Preparation Procedure for Crude Fat for HIGH fat samples To prepare meat samples with > 15% fat by weight or plant samples with > 20% fat by weight for fat extraction, follow the procedure detailed below. 1. Using a Solvent Resistant Marker, number all of the weigh tins and empty filter bags you will use during the fat extraction procedure. Use one weigh tin per filter bag. Although it is not necessary in the determination of fat, it is recommended that you weigh and record the weights of all the filter bags prior to filling them in order to calculate the moisture value for the samples. % Moisture = 100 x ((Filter Bag Weight + Sample Weight) - (Weight after drying - Tin Weight)) Sample Weight Measuring moisture can also help verify the operation of your drying oven. 2. Dry all weigh tins in an oven at 102 C±2 for 15 minutes. 3. Remove weigh tins from the oven and place them in a Weigh Tin Desiccator or in a Desiccant Pouch. 4. Allow weigh tins to cool to room temperature. 5. Weigh and record tin weights (W 4). 6. Place an empty filter bag in the Bag Holder in an open position. 7. Tare the weight of the empty filter bag and the holder together. 8. Add 1.5 2g of sample to the filter bag. Keep all particles away from the sealing area of the filter bag. 9. Record the weight of the sample (W 1). 10. Set the Heat Sealer dial to 6. (The setting may vary from sealer to sealer.) pg. 16 Rev F 1/2/18

17 11. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 3 seconds after the red sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of the filter bag (as shown to the right). If the seal is not strong, re-seal the bag. Seal 12. Repeat steps 1 11 for all filter bags that will be used in the ANKOM XT15 Extraction System. (Up to 15 bags can be processed during one procedure.) 13. Place the sealed filter bags in their corresponding weigh tins for pre-drying. 14. Dry all samples at 102 C±2 for 3 hours to remove moisture prior to the extraction. 15. Remove the weigh tins and filter bags from the oven and place them together in a Weigh Tin Desiccator. A Weigh Tin Desiccator is recommended because it will hold between 10 and 14 weigh tins with filter bags, depending on the size of the weigh tins. A Desiccant Pouch will hold about Allow the samples to cool to room temperature. This should take about minutes. 17. Re-weigh and record the weights of each weigh tin (W 4) and filter bag (W 2) immediately after removing from the Weigh Tin Desiccator. The samples are now ready for the fat extraction procedure. Rev F 1/2/18 pg. 17

18 Sample Preparation Procedure for Crude Fat for Liquid samples To prepare liquid samples for fat extraction, follow the procedure detailed below. 1. Using a Solvent Resistant Marker, number all of the empty filter bags you will use during the fat extraction procedure. Although it is not necessary in the determination of fat, it is recommended that you weigh and record the weights of all the filter bags prior to filling them in order to calculate the moisture value for the samples. % Moisture = 100 x ((Filter Bag Weight + Sample Weight) - Weight after drying) Sample Weight Measuring moisture can also help verify the operation of your drying oven. 2. Place an empty filter bag in the Bag Holder in an open position. 3. Tare the weight of the empty filter bag and the holder together. 4. Add Diatomaceous Earth (DE). 4.1 Add g of DE to the filter bag. 4.2 Using a pencil eraser (or similar sized utensil) make an indentation or a space in the DE into which the liquid sample can be added using a pipette. 4.3 Record the weight and tare. 5. Pipette g of liquid sample into the DE indentation in the filter bag. (Do NOT use more liquid sample than can be absorbed by the DE.) 6. Record the weight of the sample (W 1). 7. Set the Heat Sealer dial to 6. (The setting may vary from sealer to sealer.) 8. Seal the filter bag within 4mm of its open end. Keep the sealer arm down for 2 3 seconds after the red sealer light turns off (to cool the seal). The seal can be seen as a solid melted stripe along the top edge of the filter bag (as shown to the right). If the seal is not strong, re-seal the bag. Seal 9. Repeat steps 1 8 for all filter bags that will be used in the ANKOM XT15 Extraction System. (Up to 15 bags can be processed during one procedure.) pg. 18 Rev F 1/2/18

19 10. Dry all samples at 102 C±2 for 3 hours to remove moisture prior to the extraction. 11. Remove the samples from the oven and place them in a Desiccant Pouch. 12. Allow the samples to cool to room temperature. This should take about minutes. 13. Re-weigh each bag (W 2) immediately after removing from the Desiccant Pouch. The samples are now ready for the fat extraction procedure. Rev F 1/2/18 pg. 19

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21 Fat Extraction step-by-step procedure using the ANKOM XT15 To perform fat extraction on prepared samples, follow the procedure detailed below. 1. Turn the instrument power ON. The Display will briefly show the software version number followed by the screen to the right. PET Ether v <ENTER> The factory default solvent shown on the Display is "Pet Ether." However, the instrument computer will store in memory the last solvent selection made and use it as the default solvent shown on the Display screen every time the instrument is ready for a new run. 2. Select the solvent you want to use. 2.1 Press the Down Arrow (v) key on the Keypad until the desired solvent is shown on the Display. 2.2 Fill the solvent reservoir as needed Unscrew the Fill Cap Pour solvent into the Fill Port up to the GREEN level mark on the Solvent Sight Level. (About 500 ml of solvent is needed to reach the GREEN level mark.) Do not add solvent up to the top of the Solvent Sight Level tube or it will start flowing into the Vent Flask. If using Diethyl Ether or a solvent mixture that includes Diethyl Ether (such as the Mojonnier Mixture), you will need the O-ring Kit for High Penetration Solvents (part # X95) to replace the standard O-rings on the top of the Extraction Vessel and the Valve Kit for High Penetration Solvents (part # X55). Warning: Do NOT use Acetone in this instrument because it will cause damage to the valves. 2.3 Press ENTER on the Keypad to complete the solvent selection. The Display should now show the screen to the right. Remove/Insert Samples <ENTER> Rev F 1/2/18 pg. 21

22 3. Insert Filter Bags into the instrument. 3.1 Press down the Safety Valve Handle to release the Safety Valve Pin and rotate the Main Handle upward until it stops. 3.2 Use the Extraction Vessel Handle to pull the Extraction Vessel out. 3.3 Slide the Extraction Vessel toward you far enough to remove the white PTFE Insert or to put the Bag Holder directly into the PTFE Insert while it remains in the Extraction Vessel. PTFE Insert Bag Holder It is not necessary to remove the white PTFE Insert from the Extraction Vessel in order to put the Bag Holder in place. 3.4 Put the Bag Holder (with up to 15 bags) into the PTFE Insert. 3.5 If you removed the PTFE Insert, put it back into the Extraction Vessel, aligning the PTFE Insert slot with the Extraction Vessel pin. PTFE Insert O-ring Extraction Vessel O-ring PTFE Insert slot and Extraction Vessel pin The PTFE Insert O-ring and the Extraction Vessel O-ring must be in place before running the instrument. The PTFE Insert slot must be aligned with the Extraction Vessel pin before running the instrument. pg. 22 Rev F 1/2/18

23 3.6 Slide the Extraction Vessel into the instrument ensuring the Extraction Vessel O-ring stays in place. 3.7 Pull the Main Handle downward, closing and locking the Extraction Vessel in place. You will hear a click noise when the Safety Valve Pin locks in place. Before running the instrument, verify that the Extraction Vessel is locked in place by attempting to rotate the Main Handle upward. If it is not locked, repeat step #3.7 above. 3.8 Press ENTER on the Keypad. The Display should now show the screen to the right. (The time shown will be the last time entered.) Select time 60 (^v)... <ENTER> 4. Set the Extraction Time. 4.1 Using the arrow keys on the Keypad, select the desired Extraction time. (The recommended time is 60 minutes.) 4.2 Press ENTER on the Keypad to set the Time. The Display should now show the screen to the right. (The temperature shown will be the last temperature entered.) 5. Set the Extraction Temperature. Select temp 90 (^v)... <ENTER> 5.1 Using the arrow keys on the Keypad, select the desired Extraction Temperature. (The recommended maximum temperature is 90 C.) 5.2 Press ENTER on the Keypad to set the Temperature. The Display should now show the screen to the right. Close Vessel Water On <START> 6. Verify that the water supply is on. 7. Run the instrument. Press START on the Keypad. The instrument will run through its automatic operation, showing its status on the Display. When the instrument has completed its automatic operation, the Display will show the screen to the right. PET Ether v <ENTER> At this time, the only light shining on the Display should be the green power light. Rev F 1/2/18 pg. 23

24 8. Determine the amount of fat that was extracted. 8.1 When the Pressure Gauge reads zero, remove the Extraction Vessel by pushing down on the Safety Valve Handle to release the locking pin and then rotating the Main Handle to the open position. 8.2 Use the Extraction Vessel Handle to remove the Extraction Vessel. Warning: The Extraction Vessel interior and top will be HOT when the extraction process is complete. 8.3 Remove the PTFE Insert from the Extraction Vessel. 8.4 Remove the Bag Holder from the PTFE Insert. 8.5 Remove the filter bags from the Bag Holder. 8.6 Dry the samples for minutes in the oven at 102 ± 2 C or in the ANKOM RD Dryer set at the factory setting of 110 C. 8.7 Remove the samples from the oven or dryer and place them in a Desiccant Pouch. 8.8 Allow the samples to cool to room temperature. This should take about minutes. 8.9 Re-weigh each filter bag (W3) immediately after removing from the Desiccant Pouch. 9. Clean the fat from the bottom of the vessel using paper towel. If too much fat accumulates in the bottom of the Extraction Vessel, an overtemp fault will occur. pg. 24 Rev F 1/2/18

25 Periodic Maintenance Cleaning the Extraction Vessel Some compounds extracted during the fat extraction procedure can create a black residue in the bottom of the Extraction Vessel. The vessel can most easily be cleaned by using a drill with a long shafted stiff wire brush (ANKOM part # X107). This treatment will remove a lot of the black coating that accumulates on the bottom of the vessel. When the vessel bottom is clean, put some solvent on a cloth or paper towel and wipe down the inside of the vessel in an effort to remove any oil residue and particles that have been loosened from the vessel bottom. Do not use soap and/or water to clean the vessel. When cleaning the vessel, do not damage the level sensor tip. The tip may be cleaned with window cleaner or alcohol. Cleaning the exterior of the instrument Wipe the outside of the instrument with window cleaner or a damp sponge as necessary. Do not use Acetone to clean the instrument. Cleaning the solvent filter If filter bags are not sealed properly and sample is lost from the bag, it will be recovered in the solvent filter in the exhaust line. To clean the solvent filter, follow the procedure detailed below. 1. Remove the rear cabinet of the instrument. 2. Unscrew the compression fitting. 3. Unscrew the filter body. 4. Unscrew and clean the metal filter screen. 5. Reassemble the filter and compression fitting. Make sure the valve body is tight and the PTFE washer has not come out of the filter body. Filter body Compression Fitting Rear View of instrument with cabinet back removed Rev F 1/2/18 pg. 25

26 Cleaning the water filter To clean the water filter (located on the water supply line), follow the procedure detailed below. 1. Unscrew the filter from the instrument. 2. Unscrew the filter clamp from the water supply line. 3. Use compressed air to blow backwards through the filter (blow opposite the arrow on the filter). If the filter is clogged, replace it with ANKOM part # X Reconnect the filter and fittings. 5. Turn on the water and check for leaks. Replacing the Fuses To replace the fuses in the ANKOM XT15 Extraction System, follow the procedure detailed below. 1. Turn off the instrument power and unplug the Power Cord from the outlet. 2. Remove the two screws on the Top Cover. 3. Swing the Top Cover down exposing the electrical compartment. 4. Locate the Fuse Holders on the terminal strip. There are two fuses within the instrument; one for each pole ( V 10A or V 5A). Filter Fuse Holders 5. Press the tabs to lift the Fuse Holders up. Tab to open Fuse Holder Fuse 6. Replace the fuses and close the Fuse Holders completely. 7. Close the Top Cover and secure it with the two screws that you previously removed. Replacing the Charcoal Filter The Charcoal Filter should be replaced every 3 months, or after 100 extraction operations using the instrument. To replace the filter, remove the old one from the vent line and install the new one. pg. 26 Rev F 1/2/18

27 Troubleshooting & Replacement Parts The ANKOM technology web site has the most current troubleshooting and replacement parts information. Therefore, if you have any questions about the operation of your ANKOM XT15 Extraction System, or if you need replacement parts, please visit our web site at Rev F 1/2/18 pg. 27

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29 Diagnostics To help solve problems that may occur while running the ANKOM XT15 Extractor, the following computer controlled Diagnostics are available and accessible through the Display and Keypad. 1. Toggle Outputs (Q0 Heater Relay and Q1 - Q7 valves) 2. Check Temperature and Level Sensors 3. Check the state of the ETS (Emergency Temperature Shut-off switch) relay Access Diagnostics Screen When the instrument is powered up or when the instrument finishes a run, the screen to the right is displayed. PET Ether v <ENTER> The factory default solvent shown on the Display is "Pet Ether." However, the instrument computer will store in memory the last solvent selection made and use it as the default solvent shown on the Display screen every time the instrument is ready for a new run. To access the Diagnostics Screen, press the Down Arrow button (v) on the Keypad four times. The screen to the right will be displayed. From the Diagnostics Screen you can access all of the available diagnostics. Diagnostics ^ <ENTER> Toggle Outputs To toggle outputs, follow the procedure below. 1. With the Diagnostics screen displayed, press the ENTER button on the Keypad. The screen to the right will be displayed. Outputs <ENTER> Exit <STOP> v If you press the STOP button on the Keypad while this screen is displayed, you will go back to the Diagnostics screen. If you press the Down Arrow button (v) on the Keypad while this screen is displayed you will go to the next group of diagnostics (Analog). 2. With the Outputs screen displayed, press the ENTER button on the Keypad. The screen to the right will be displayed. Heater v (Q0) <ENTER> 3. Press the Down Arrow button (v) on the Keypad to select the heater relay (Q0) or valve (Q1 - Q7) you want to toggle. With your selection made (in this example we selected the heater relay Q0), press the ENTER button on the Keypad. The screen to the right will be displayed. Heater (Q0) OFF ENTER to toggle Rev F 1/2/18 pg. 29

30 4. Press the ENTER button on the Keypad to turn ON the heater relay (Q0). The screen to the right will be displayed. Heater (Q0) ENTER to toggle ON 5. Press the ENTER button on the Keypad to turn OFF the heater relay (Q0). The screen to the right will be displayed. Heater (Q0) OFF ENTER to toggle Warning: If the heater relay (Q0) is left ON, the Extraction Vessel interior will become very HOT and within five minutes the instrument ETS switch will trip resulting in the Fault screen shown here. Diagnostics ETS Fault <ENTER> From this screen press ENTER to get back to the main Diagnostics screen. 6. Press the STOP button on the Keypad to go back to the main Outputs screen (shown to the right). Outputs <ENTER> Exit <STOP> v 7. Press the STOP button on the Keypad to go back to the main Diagnostics screen (shown to the right). Diagnostics ^ <ENTER> 8. Press the Up Arrow button (^) on the Keypad to exit Diagnostics. Press the Up Arrow button (^) on the Keypad up to three more times to return to your solvent screen (example shown to the right). PET Ether v <ENTER> When you exit Diagnostics, heater relay Q0 turns OFF and valves Q1 - Q7 return to the closed position. pg. 30 Rev F 1/2/18

31 Check Temperature and Level Sensors To check the Temperature and Level Sensors, follow the procedure below. 1. Access the Diagnostics screen (see the "Access Diagnostics Screen" section for details). Diagnostics ^ <ENTER> 2. With the Diagnostics screen displayed, press the ENTER button on the Keypad. The screen to the right will be displayed. Outputs <ENTER> Exit <STOP> v 3. Press the Down Arrow button (v) on the Keypad. The screen to the right will be displayed. Analog <ENTER> Exit <STOP> ^ v 4. Press the ENTER button on the Keypad. The screen to the right will be displayed showing the current Temperature and Level Sensor readings. Temp Lvl Sense 20 C 10.00v When the instrument has not been run for more than two hours, the temperature should read the ambient temperature of the room. If the temperature reading does not show ambient temperature when the instrument is cool, contact ANKOM Technology. When the Level Sensor in the instrument Vessel is not covered by liquid, the Level Sensor should read 10.00v. If the reading is less than 10.00v and the Level Sensor is not covered by liquid, clean the Level Sensor Tip inside the Vessel with a cotton swab and alcohol. If the Level Sensor reading is between v after cleaning the tip, the instrument will run, but you should consider replacing the tip. Contact ANKOM Technology for more information. When the instrument Vessel is filled with liquid that covers the Level Sensor, the Level Sensor should read less than 3.00v. If the Level Sensor reading is more than 3.00v when the sensor is covered by liquid, contact ANKOM Technology. If you fill the Vessel with water during Diagnostics, wipe the Vessel dry before running the instrument. 5. Press the STOP button on the Keypad to go back to the Analog screen (shown to the right). Analog <ENTER> Exit <STOP> ^ v 6. Press the STOP button on the Keypad to go back to the main Diagnostics screen (shown to the right). Diagnostics ^ <ENTER> Rev F 1/2/18 pg. 31

32 7. Press the Up Arrow button (^) on the Keypad to exit Diagnostics. Press the Up Arrow button (^) on the Keypad up to three more times to return to your solvent screen (example shown to the right). PET Ether v <ENTER> Check the state of the ETS (Emergency Temperature Shut-off switch) relay To check the state of the ETS relay, follow the procedure below. 1. Access the Diagnostics screen (see the "Access Diagnostics Screen" section for details). Diagnostics ^ <ENTER> 2. With the Diagnostics screen displayed, press the ENTER button on the Keypad. The screen to the right will be displayed. Outputs <ENTER> Exit <STOP> v 3. Press the Down Arrow button (v) on the Keypad. The screen to the right will be displayed. Analog <ENTER> Exit <STOP> ^ v 4. Press the Down Arrow button (v) on the Keypad. The screen to the right will be displayed. Inputs <ENTER> Exit <STOP> ^ 5. Press the ENTER button on the Keypad. The screen to the right will be displayed showing the state of the ETS relay. If the ETS relay is "OFF", contact ANKOM Technology. ETS relay - ON 6. Press the STOP button on the Keypad to go back to the Inputs screen (shown to the right). Inputs <ENTER> Exit <STOP> ^ 7. Press the STOP button on the Keypad to go back to the main Diagnostics screen (shown to the right). Diagnostics ^ <ENTER> 8. Press the Up Arrow button (^) on the Keypad to exit Diagnostics. Press the Up Arrow button (^) on the Keypad up to three more times to return to your solvent screen (example shown to the right). PET Ether v <ENTER> pg. 32 Rev F 1/2/18

33 Appendix A Electrical Diagram (pg. 1 of 3) Rev F 1/2/18 pg. 33

34 Appendix A Electrical Diagram (pg. 2 of 3) pg. 34 Rev F 1/2/18

35 Appendix A Electrical Diagram (pg. 3 of 3) Rev F 1/2/18 pg. 35

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