CelCulture CO2 Incubators CelCulture for IVF. Cradle for Beautiful Cells. Cradle for Optimal Embryo Incubation. Designed in the USA

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1 1 CO2 Incubators Cradle for Beautiful Cells CelCulture CO2 Incubators CelCulture for IVF Cradle for Optimal Embryo Incubation Designed in the USA

2 2 CelCulture Incubators The Incubator has a vital role in providing an optimal environment in embryo development during IVF and other ART procedures. Sleek, reliable and intuitive, Esco CelCulture incubators are packed with outstanding features such as rapid parameter recovery, ISO Class 5 Cleanliness, ISOCIDE TM antimicrobial coating, optional Inner Door Kit that reduces contamination risk, and other accessories for specialized applications. Key Features CelCulture Incubators Cradle for Optimal Embryo Incubation ULPA Filter % efficient, superior to conventional HEPA filters Filters air continuously Chamber returns to ISO Class 5 cleanliness in 13 minutes upon door closing to prevent contamination Perforated shelving to improve uniformity Anti-tip Stainless steel Built-in grip Dismantles without tools for easy cleaning Direct Heat & Air Jacket Fast and uniform heating Rapid temperature recovery without overshoot Air jacket improves chamber stability Shelving Duct Work Directs air flow for rapid recovery and excellent uniformity Easily removed for cleaning Water Pan Precisely heated by base heater to provide high humidity Gentle airflow over water surface accelerates humidity recovery Inner Door Kit Divides the incubator into different chambers Minimizes recovery times and contamination risk CelCulture Incubators available in 3 sizes, 50 L, 170 L, and 240 L.

3 3 Sensor for suppressed O2 model Long life Stable output signal Not affected by concentration inside the chamber Door Switch Automatically turns off the blower and gas supply when the door is opened Top Cover Provides quick access to electrical panel components CO2 Sensor IR Sensor Single-beam, dual-wavelength IR sensor is drift-free Auto-zeroing Smartsense TM Microprocessor Interface Intuitive, fully-equipped control and monitoring system Blower Gentle airflow in chamber improves recovery and uniformity Outer Door Reversible Heated to prevent condensation Sample Port Allows direct measurement of chamber atmosphere such as temperature and concentration Leveling Feet Easily adjustable Glass Door For observing sample cells inside the chamber during operation Door Latch To lock / unlock the glass door Note: For 50 L Model, no top plenum, bottom plenum, blower & ULPA Filter. Quality Esco Construction - Electrogalvanized steel with white oven-baked epoxy-polyester antimicrobial powder-coated finish. - External surfaces are powder coated with Esco to eliminate 99.9% of surface bacteria within 24 hours of exposure. - Ensures a healthier, safer and cleaner lab environment.

4 4 Celculture Incubators for IVF Achieve optimal embryo growth CelCulture Incubators now has a specific line of incubators for applications in IVF. These are packed with outstanding features to ensure stable environment for optimal embryo growth and faster parameter recovery every time the door is opened. Faster Temperature,, and Recovery Time CelCulture Incubators for IVF are equipped with a sophisticated firmware and robust hardware components to facilitate faster recovery time for temperature,, and. Faster recovery times render less stress to culture to ensure the integrity and full development of the embryos. Fully redesigned sensor location to facilitate for a faster recovery of temperature inside the chamber. Infrared sensor monitors and controls inside the chamber, while sensor maintains the suitable oxygen concentration for optimal incubation and growth of embryos. Maintaining Low Levels of Oxygen for Optimal Embryo Growth The Suppressed Model of the CelCulture is built to mimic the natural environment of developing embryos by maintaining the low levels of oxygen. The galvanic cell type sensor ensures accurate control and monitoring of hypoxic conditions inside the chamber. Inner Door Kit Feature An optional feature that helps to hasten the recovery of parameters and prevent cross-contamination. The 50 L chamber is divided into two sections while the 170 L and 240 L chambers are divided into four sections. Each section has dedicated door. Access Ports in Key Positions Two access ports located at the front and rear of the chamber provide an easy route for calibration and validation of the chamber parameters. Additional sensors can also be introduced into the chamber through these access ports. Maximum Protection Against Contaminants - Moist heat decontamination cycle - Gas filters - ISOCIDE TM antimicrobial coating For IVF applications, Esco CelCulture incubators are certified embryo-safe. Rigorously tested with the Mouse Embryo Assay (MEA), the CelCulture remarkably has 100% embryo survival. The Mouse Embryo Assay (MEA) is the de facto standard test done to demonstrate that a procedure or an article of equipment is safe to use for manipulating human embryos (e.g., in vitro fertilization or IVF).

5 5 Guide to Models CCL Volume Code Sensors Code Electrical Rating Code Internal Chamber Code 50 Liters IR sensor ( and Control) T 230 VAC, 50/60 Hz 8 SS Liters VAC, 50/60 Hz 9 100% Copper Cu 240 Liters 240 General Specifications CCL-050T-_-IVF CCL-170T-_ CCL-240T-_ TEMPERATURE Temperature Control Method Direct heat & air jacket using Microprocessor PID Temperature Range, C Amb. +3 to 60 Recovery Time 4 mins 6 mins 6 mins Control System Microprocessor PID Range, % 0-20 Sensor Infrared (IR) Sensor Recovery Time Suppressed model: 6 minutes after 30 sec door opening Suppressed model: 5 minutes after 1 min door opening Suppressed model: 5 minutes after 1 min door opening SPECS (FOR SUPPRESSED MODEL) Control System Microprocessor PID Range, % % Sensor Galvanic Cell Type Recovery Time at 5.0% by volume At 5.0% by volume: 8 minutes after 30 sec door opening At 5.0% by volume: 10 minutes after 1 min door opening At 5.0% by volume: 12 minutes after 1 min door opening HUMIDITY Humidification Method Humidity pan Humidity Range, % RH Up to 97% PHYSICAL CONSTRUCTION External Dimensions (W x D x H) 500 x 500 x 655 mm (19.7 x 19.7 x 25.8 ) 660 x 660 x 900 mm (26.0 x 26.0 x35.4 ) 750 x 665 x 900 mm (29.5 x 26.2 x 35.4 ) Gross Internal Dimensions (W x D x H) 345 x 375 x 390 mm (13.6 x 14.8 x 15.4 ) 505 x 530 x 635 mm (19.9 x 20.9 x 25.0 ) 595 x 620 x 635 mm (23.4 x 24.4 x 25.0 ) Shipping Weight 70 kg (154.3 lbs) 120 kg (264.6 lbs) 155 kg (341.7 lbs) Interior Volume 50 L (1.8 ft 3 ) 170 L (5.7 ft 3 ) 240 L (8.5 ft 3 ) CONTAMINATION CONTROL Contamination Control Methods 1) Main body is electrogalvanized steel with ISOCIDE TM antimicrobial coating; 2) Moist 90 C OVERNIGHT decon. cycle (HPA validated); 3) 0.2 micron in-line filter for gas inputs; 4) ULPA filter* * Not available for 50 L

6 6 Options and Accessories Miri GA / and Temperature Validation Unit Stacking Kit For stacking of one incubator on top of another incubator Humidity Display For monitoring of the Relative Humidity inside the chamber. Works from 70 C to 180 C Back up This option allows two tanks of to be connected to the incubator Roller Base With casters for mobility of your incubators. Floor Stand with Adjustable Feet Nominal range of 180 mm to 250 mm (7.1 to 9.8 ) Floor Stand with Casters Support stand raises the incubator to a height of 700 mm (27.6 ) above the floor Sealed Inner Door Kit Defined sections of the incubator without disturbing the inner atmosphere Analog Output Used for connecting the incubator to an output analog signals representing the temperature, gas concentration and relative humidity. Additional Stainless Steel Shelf (for SS Chamber) Maximize your incubator space by adding more shelves. Can add 1 extra shelf for 50 L model, 3 more shelves for 170 L and 240 L models. Chart Recorder Easy-to-read graph of data vs. time Voyager Software Kit PC-based software for remote monitoring, datalogging and programming. Other Esco Embryo Incubators Miri Multi-room Benchtop Incubator The overall design provides embryo culture in a minimum-stress environment. The 6-chamber format prevents cross-contamination while HEPA+VOC filtration and UV light clean the airflow of the incubator. The chambers are also independent of each other; hence, any disruption such as temperature drop when opening the lid will have no impact on the rest of the system. Miri TL Multi-room Incubator Time-Lapse Imaging has given birth to a new concept in the IVF laboratory, termed morphokinetics. It allows embryologists to see significant morphological events that might not be seen through conventional scoring. With Miri TL, you can watch the embryos grow and develop throughout the entire culture process. There s no need to take your dishes out for scoring.

7 7 Ordering Information MODEL ITEM CODE DESCRIPTION CCL-050T-8-IVF CelCulture Incubator, 50 L, IR Sensor, Control, Moist Heat Decon, 115 VAC, 50/60 Hz, for IVF CCL-050T-9-IVF CelCulture 50 L, IR Sensor, Control, Control, Moist Heat Decon, 115 VAC, 50/60 Hz, for IVF CCL-170T CelCulture Incubator, 170L, IR Sensor, Control, Control, ULPA, Moist Heat Decon, 230 VAC, 50/60 Hz CCL-170T CelCulture Incubator, 170 L, IR Sensor, Control, Control, ULPA, Moist Heat Decon,115 VAC, 50/60 Hz CCL-240T CelCulture Incubator, 240 L, IR Sensor, Control, Control, ULPA, Moist Heat Decon, 230 VAC, 50/60 Hz CCL-240T CelCulture Incubator, 240 L, IR Sensor, Control, Control, ULPA, Moist Heat Decon,115 VAC, 50/60 Hz CCL-050T-8-Cu CelCulture Incubator, Pure Copper Chamber, 50 L, IR sensor, Control, Moist Heat Decon, 230 VAC, 50/60 Hz CCL-050T-9-Cu CelCulture Incubator, Pure Copper Chamber, 50 L, IR sensor, Control, Moist Heat Decon, 115 VAC, 50/60 Hz CCL-170T-8-Cu CelCulture Incubator, Pure Copper Chamber, 170 L, IR Sensor, Control, ULPA, Moist Heat Decon 230 VAC, 50/60 Hz CCL-170T-9-Cu CelCulture Incubator, Pure Copper Chamber, 170 L, IR Sensor, Control, ULPA, Moist Heat Decon 115 VAC, 50/60 Hz CCL-240T-8-Cu CelCulture Incubator, Pure Copper Chamber, 240 L, IR Sensor, Control, ULPA, Moist Heat Decon, 230 VAC, 50/60 Hz CCL-240T9-Cu CelCulture Incubator, Pure Copper Chamber, 240 L, IR Sensor, Control, ULPA, Moist Heat Decon, 115VAC, 50/60 Hz ACCESSORIES ITEM CODE DESCRIPTION ACCESSORIES ITEM CODE DESCRIPTION COA Humidity Display, Factory-installed COA-1001-F Humidity Display, Field-installed Kit COA Backup (Tank Switcher), Factory-installed COA-1002-F Backup (Tank Switcher), Field-installed COA Reversed Door Swing, Factory-installed COA Analog Outputs, Factory-installed COA-1005-F Analog Outputs, Field-installed COA-2005-F COA-2026-F COA-2027-F Stage Gas Regulator for / N 2 Choose one of the connectors below: CGA 320 Connector (US standard) BP-BS34-#8-NT4 Connector (British standard) G5/8-RH Connector (China standard) Accessory, Extra Copper Shelf, with 2 Support Rails (For 50L) Accessory, Extra Copper Shelf, with 2 Support Rails (For 170L) COA Sealed Inner Door Kit for 170 L(4 Glass Doors with Latches), Factory-installed COA-2028-F Accessory, Extra Copper Shelf, with 2 Support Rails (For 240L) COA-1006-F Sealed Inner Door Kit for170 L (4 Glass Doors with Latches), Field-installed COA-2024-F Extra Shelf (50 L, stainless steel) COA N 2 Back-up (Tank Switcher), Factory-installed COA-1007-F N 2 Back-up (Tank Switcher), Field-installed COA-2018-F Roller Base (50 L) COA-2001-F Roller Base (170 L) COA-2019-F Roller Base (240 L) COA-2020-F COA-2002-F COA-2021-F COA-2022-F Floor Stand 200 mm (8.0 ) with Adjustable Feet (50 L) Floor Stand 200 mm (8.0") with Adjustable Feet (170 L) Floor Stand 200 mm (8.0 ) with Adjustable Feet (240 L) Floor Stand 700 mm (27.6") with Casters (50 L) COA-2007-F Extra Shelf (170 L, stainless steel) COA-2025-F Extra Shelf (240 L, stainless steel) COA-2008-F COA-2010-F COA-2016-F COA-2017-F Stacking Kit (one set included with every unit purchased) Electronic Analyzer, For / Temp Measurement (with Temperature Probe) Electronic + Analyzer, For / / Temperature Measurement Electronic + + RH Analyzer, For / / RH / Temperature Measurement COA-2011-F IQ / OQ Documentation COA-2012-F " Chart Recorder, Temp, 115/230 VAC, 50/60 Hz COA-2003-F COA-2023-F COA COA-2030-F Floor Stand 700 mm (27.6") with Casters (170 L) Floor Stand 700 mm (27.6 ) with Casters (240 L) Sealed Inner Door Kit, 50 L, 2 Glass Doors with Latches, Factory-installed Sealed Inner Door Kit, 50 L, 2 Glass Doors with Latches, Field-installed COA-2013-F COA-2014-F COA-2015-F " Chart Recorder, Temp / Temp, 115/230 VAC, 50/60 Hz 6" Chart Recorder, Temp/RH, 115/230 VAC, 50/60 Hz Inner Door Shelving Kit for 170 L (4 sets with total 12 mini-shelves for one incubator) Voyager Voyager Software Kit COA Sealed Inner Door Kit, 240 L, 4 Glass Doors with Latches, Factory-installed MRI-GA Miri GA / / Temp Validation Unit, VAC, 50/60 Hz

8 ESCO GLOBAL NETWORK Sales/Service Subsidiary Companies Distributors Factories R&D Centers Regional Distribution Centers Esco Medical Products: Anti-Vibration Table (AVT) Incubators Culture and Fertilization Dishes Fertilisafe IVF Workstation Miri Benchtop Multi-room Incubators Miri TL Time Lapse Embryo Incubators Miri GA (Gas and Temperature Validation Unit) Miri GA Mini (Gas Analyzer) Semi Closed Environment (IVF/ICSI) Infertility is viewed as a problem that has social, psychological, and economic impacts to the afflicted individuals and couples. It is a global concern that knows no race or creed. It has been estimated that 1 in 6 couples would struggle with infertility at least once in their lifetime. Esco Medical is one of the divisions of the Esco Group of Companies, the other two being the laboratory and pharmaceutical equipment divisions. Esco is now targeting innovative technological solutions for fertility clinics and laboratories. Esco Medical is positioned to become a leading manufacturer and innovator of high-quality equipment such as long-term embryo incubators, ART workstations, anti vibration table, time- lapse incubator and etc. Esco Medical products are designed to develop with the Silent Embryo Hypothesis as a guiding principle. The Silent Embryo Hypothesis states that the less disturbed an embryo can remain, the better its developmental potential will be. Most of our products are designed in Denmark and made in the EU. The primary focus of this division is to increase pregnancy success rates and patient satisfaction. Esco Micro Pte. Ltd. 21 Changi South Street 1 Singapore Tel Fax mail@escoglobal.com Esco Technologies, Inc. 903 Sheehy Drive, Suite F, Horsham, PA 19044, USA Toll-Free USA and Canada Tel Fax eti.sales@escoglobal.com Esco Global Offices: Bahrain Bangladesh China India Indonesia Italy Japan Malaysia Philippines Russia Singapore South Africa South Korea Thailand United Kingdom USA Vietnam _CCL-IVF (Esco Medical) Brochure_A4_ Esco can accept no responsibility for possible errors in catalogues, brochures and other printed materials. Esco reserves the right to alter its products and specifications without notice. All trademarks and logotypes in this material are the property of Esco and the respective companies. escoglobal.com LIVE SUPPORT Esco Micro Pte Ltd Certificate No: PT. Esco Bintan Indonesia Certificate No: /E PT Esco Bintan Indonesia Cert. No: Q2N

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