Foodstuffs - Determination of vitamin C by HPLC

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1 Irish Standard I.S. EN 14130:2003 Foodstuffs - Determination of vitamin C by HPLC NSAI 2003 No copying without NSAI permission except as permitted by copyright law.

2 This document was published under the authority of the NSAI and comes into effect on: 12 September, 2003 EN 14130:2003 ICS number: Swift Square, Northwood, Santry Dublin 9 T F E W T F W standards.ie Údarás um Chaighdeáin Náisiúnta na héireann

3 EUROPEAN STANDARD NORME EUROPÉENNE EUROPÄISCHE NORM I.S. EN 14130:2003 EN June 2003 ICS English version Foodstuffs - Determination of vitamin C by HPLC Produits alimentaires - Dosage de la vitamine C par CLHP Lebensmittel - Bestimmung von Vitamin C mit HPLC This European Standard was approved by CEN on 21 April CEN members are bound to comply with the CEN/CENELEC Internal Regulations which stipulate the conditions for giving this European Standard the status of a national standard without any alteration. Up-to-date lists and bibliographical references concerning such national standards may be obtained on application to the Management Centre or to any CEN member. This European Standard exists in three official versions (English, French, German). A version in any other language made by translation under the responsibility of a CEN member into its own language and notified to the Management Centre has the same status as the official versions. CEN members are the national standards bodies of Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and United Kingdom. EUROPEAN COMMITTEE FOR STANDARDIZATION COMITÉ EUROPÉEN DE NORMALISATION EUROPÄISCHES KOMITEE FÜR NORMUNG Management Centre: rue de Stassart, 36 B-1050 Brussels 2003 CEN All rights of exploitation in any form and by any means reserved worldwide for CEN national Members. Ref. No. EN 14130:2003 E

4 EN 14130:2003 (E) I.S. EN 14130:2003 Contents page Foreword Scope Normative references Principle Reagents Apparatus Procedure Calculation Precision Test report...10 Annex A (informative) Example of a chromatogram...11 Annex B (informative) Precision data...12 Bibliography

5 I.S. EN 14130:2003 EN 14130:2003 (E) Foreword This document (EN 14130:2003) has been prepared by Technical Committee CEN /TC 275, "Food analysis - Horizontal methods", the secretariat of which is held by DIN. This European Standard shall be given the status of a national standard, either by publication of an identical text or by endorsement, at the latest by December 2003, and conflicting national standards shall be withdrawn at the latest by December Annexes A and B are informative. WARNING The use of this standard can involve hazardous materials, operations and equipment. This standard does not purport to address all the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. According to the CEN/CENELEC Internal Regulations, the national standards organizations of the following countries are bound to implement this European Standard: Austria, Belgium, Czech Republic, Denmark, Finland, France, Germany, Greece, Hungary, Iceland, Ireland, Italy, Luxembourg, Malta, Netherlands, Norway, Portugal, Slovakia, Spain, Sweden, Switzerland and the United Kingdom. 1 Scope This European Standard specifies an HPLC-method for the determination of vitamin C in foodstuffs. Vitamin C is the sum of L(+) ascorbic acid and dehydro L(+) ascorbic acid. 2 Normative references This European Standard incorporates by dated or undated reference, provisions from other publications. These normative references are cited at the appropriate places in the text and the publications are listed hereafter. For dated references, subsequent amendments to or revisions of any of these publications apply to this European Standard only when incorporated in it by amendment or revision. For undated references the latest edition of the publication referred to applies (including amendments). EN ISO 3696, Water for analytical laboratory use Specification and test methods (ISO 3696:1987). 3 Principle Vitamin C is extracted from the sample to be analysed using metaphosphoric acid solution. A reducing solution is used to transform dehydro L(+) ascorbic acid to L(+) ascorbic acid. Total L(+) ascorbic acid content is determined by HPLC with a UV detection at 265 nm [1], [2]. 4 Reagents 4.1 General During the analysis, unless otherwise stated, use only reagents of recognised analytical grade and water of at least grade 1 according to EN ISO 3696, or double distilled water. 3

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