Table of Contents. Why Raman? and Why Now? 2. The BaySpec Advantage 3. Customized Instruments 14. OEM Partnership 14

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1 Table of Contents Why Raman? and Why Now? 2 The BaySpec Advantage 3 Xantus TM -0 Raman Analyzer 4 Xantus TM -1 Raman Analyzer 6 Xantus TM -2 Raman Analyzer 8 Portability TM -1 Raman Analyzer 10 RamSpec TM -1 Raman Analyzer 12 Customized Instruments 14 OEM Partnership 14 Accessories for Raman Analyzers 15 Software: Micro 20/20 16 Applications of Raman Spectroscopy 18 Raman Analyzer Selection Guide 20 성경포토닉스 l 042)

2 Why Raman? and Why Now? A Primer on Raman Spectroscopy The Raman effect occurs when light impinges upon a molecule and interacts with the electron cloud that binds that molecule. While most of the incident light is scattered with its wavelength unchanged (i.e., elastically scattered), a small fraction (on the order of 10-6 ) of the light is inelastically scattered and exits with longer wavelength and simultaneously promote the molecule into a more energetic vibrational state. An even smaller fraction of the light picks up energy from the molecule and leaves with a shorter wavelength. This effect was named after one of its discoverers, Sir C. V. Raman, who, in 1928, accomplished his observation with sunlight and filters. A change in the molecular polarizability or deformation of the electron cloud with respect to the vibrational coordinate is required for the molecule to exhibit the Raman effect. The amount of the polarizability change will determine the Raman scattering intensity, whereas the shift in wavelength (called the Raman shift) is equal to the vibrational level that is involved. When the shift is to longer wavelength it is called the Stokes shift, and when it is to shorter wavelength it is called the anti- Stokes shift. The location and intensity of the Raman lines are determined by the underlining molecular structure and are unique to the molecule and its environment, thus the Raman spectrum bears a molecular spectral finger print. For Raman spectroscopy both excitation and detection are achieved in the visible or near-infrared range, but the obtained spectral information is in the infrared range (difference between excitation and detection). Detection of light in the visible/near infrared is generally much easier than in the infrared since it is less susceptible to thermal noise. Raman spectroscopy is a powerful analytical technique owning to its nondestructive nature and simplicity in sample preparation and handling. However, its full potential could not be reached until now. The reasons may be traced back to Sir C. V. Raman s original observations made in 1928; in his Communications to Nature he commented that the effect s excessive feebleness and the need for very powerful illumination. Lasers, invented in the 1960 s, certainly qualified as powerful illumination sources. However, for a long time lasers were bulky, expensive, and required experts to operate. A laser Raman spectrometer from mid-1980s Sensitive detectors and other optical components such as line-ruled gratings were also scarce and expensive. Thus Raman spectrometers were reserved for sophisticated scientific research and not accessible for routine analytical applications. Why Now? In the late 1990 s the buildup of the optical telecommunications infrastructure brought down price barriers for many lasers while reducing their physical dimensions. Holographic optical components are also much more accessible than before. Together with compact yet powerful computers/microprocessors high performance Raman spectrometers can be made in portable, even handheld, form factors at a fraction of the cost of traditional research instruments today. The time for Raman is NOW. A handheld Raman spectrometer from BaySpec in 2009 성경포토닉스 l 042)

3 The BaySpec Advantage Volume Phase Grating (VPG ) Central to any dispersive spectrometer is the grating, the dispersive element. Transmission VPGs offer generally higher efficiencies than traditional reflective gratings. For low signal applications this is a significant performance edge. BaySpec s Raman spectrometers are equipped with VPGs so that they have higher optical throughput than similar products on the market. These VPGs are designed and manufactured on site at BaySpec. The spectral coverage of the spectrometers is solely dictated by customers needs and not limited by the availability of gratings from third party vendors. VPG s manufactured at BaySpec Schematic Diagram of a NIR Dispersive Spectrometer based on a Transmissive Holographic Volume Phase Grating (VPG ) Right Detector for the Right Job BaySpec offers a complete line of array detectors in the visible and near infrared that are sensitive to 1700 nm, 2200 nm, or 2500 nm. Noise from dark currents is a challenging issue for NIR detectors, especially when the spectral range is extended to mid- IR. To ensure exceptional signal to noise ratio in every spectral range, BaySpec s detectors run either athermally, or thermoelectrically cooled to 20 C. For even more demanding applications the detector array can be cooled to 55 C. 성경포토닉스 l 042)

4 Xantus TM -0 Raman Analyzer Palm sized Reliable Ruggedized Battery-operated Ideal for: Chemical solvents identification In-line/at-line sensors Field inspections Surface Enhanced Raman (SERS) Teaching labs Quality Control/Assurance With a footprint approximately a third of a composition book and weighing than 2 lbs, Xantus TM -0 is an affordable, yet complete Raman spectrometer. It is available in two versions, one connected to a smart phone for remote wireless operation and one integrated with a Personal Digital Assistant (PDA). In addition to on-board data processing functions, the Xantus TM -0 can transmit data to centralized facilities via cellular or WiFi networks, thus enabling real time communication and decision making in mission critical situations. Tailored for Raman Each Xantus TM -0 is equipped with a 785 nm laser stabilized for Raman spectroscopy. Output power: >80 mw. Linewidth: 0.2 nm FWHM typical. 1 s integration time At the heart of every Xantus TM -0 is a transmissive VPG that delivers unsurpassed optical throughput. The spectrograph is customized to cover the cm -1 range which is the most chemically informative spectral region, thus leaving no pixels wasted. The detector is a 1024 element CCD array that delivers high sensitivity cost effectively. Xantus TM -0 runs on BaySpec s Micro 20/20 software that has an intuitive, streamlined user interface and can output data in.csv format for easy importing to other applications. Baseline correction function is intelligently built in, which greatly facilities reduction in fluorescence interference and account for drift in background Xantus TM -0 not only has no moving parts but also has its sensitive optical engine shock-resistant mounted, resulting in a ruggedized instrument that has withstood the rigors of field testing. 성경포토닉스 l 042)

5 Xantus TM -0 Raman Analyzer Bring Instrumentation to the Sample The Xantus TM -0 can perform measurements either via direct focusing or through a vial holder. For hard to reach spaces where a fiber collection bundle is more appropriate a fiber optic adapter is available. The Xantus TM -0 can handle samples in liquid, gel, powder, or solid forms, under laboratory conditions, or field environments, thus fulfilling the promise of bring instrument to the sample. Xantus: a hummingbird species native to Baja California and often spotted on the California coast. Hummingbirds are among the smallest of birds, can hover in mid-air by flapping their wings times per second and fly at speeds exceeding 15 m/s (54 km/h, 34 mi/h). Xantus has a wide spectral vision up to near-uv. Specifications 600 Gasoline Methanol Raman spectra of gasoline, methanol, and their mixture 1800 Part Number HRAM-0A-0785 HRAM-0B-0785 Size Dimensions (mm 3 ) 85 x 190 x x 195 x 60 Weight 1 kg (2.2 lbs.) 1.5 kg (3 lbs.) Excitation Source Wavelength 785 nm Bandwidth 0.2 nm typ./ 0.3 nm max. (FWHM) Output power ~80 mw Spectrograph Grating technology Transmission Volume Phase Grating (VPG ) Range (785nm) cm -1 Spectral resolution ~15-17 cm -1 Stray light rejection 0.05% Detector Type CCD Number of pixels 1024 Pixel size 14 µm Cooling temperature Ambient Integration time sec. Readout speed 250 khz Digitalized output 16-bit Dynamic range 2000:1 typ. Quantum Efficiency (max.) >90% Electronics Interface USB 2.0 Input power Battery operated Trigger mode 5 V TTL edge or level (optional) Battery life 4 hrs Software GUI Micro 20/20 for Windows CE SDK N/A Spectral libraries User built 성경포토닉스 l 042)

6 Xantus TM -1 Raman Analyzer Handheld Reliable Ruggedized Battery-operated Ideal for: Incoming materials inspection Gemstone authentication Mineral identification Food safety evaluation Narcotics & drugs Teaching labs Quality Control/Assurance With a laser that is capable of delivering 500 mw of power and a thermoelectrically cooled CCD array detector, Xantus TM -1 is a high performance Raman spectrometer in an ultra compact package. It can accomplish some of the most challenging analytical tasks traditionally reserved for research grade instruments, while field ready with no need for external power or cooling. In addition to on-board data processing functions, the Xantus TM 1 can transmit data to centralized facilities via WiFi networks, thus enabling real-time communication and decision making in mission critical situations. Tailored for Raman Each Xantus TM -1 is equipped with a 785 nm laser stabilized for Raman spectroscopy. Output power: >500 mw. Linewidth: 0.2 nm FWHM typical. At the heart of every Xantus TM -1 is a transmissive VPG that delivers unsurpassed optical throughput. The spectrograph is customized to cover the cm -1 range which is the most chemically informative spectral region, thus leaving no pixels wasted. The detector is a 1024 element CCD array thermoelectrically cooled to 20 C that drastically reduces dark currents and delivers superior signal to noise ratios. 1 s integration time Xantus TM -1 runs on BaySpec s Micro 20/20 software that has an intuitive, streamlined user interface and can output data in.txt,.csv, or.spc format. Baseline correction function is intelligently built in, which greatly facilities reduction in fluorescence interference and account for drift in background Xantus TM -1 not only has no moving parts but also has its sensitive optical engine shock-resistant mounted, resulting in a ruggedized instrument that has withstood the rigors of field testing. 성경포토닉스 l 042)

7 Bring Instrumentation to the Sample Xantus TM -1 Raman Analyzer The Xantus TM -1 can perform measurements either via direct focusing or through a vial holder. For hard to reach spaces where a fiber collection bundle is more appropriate a fiber optic adapter is available. The Xantus TM -1 can handle samples in liquid, gel, powder, or solid forms, under laboratory conditions, or field environments, thus fulfilling the promise of bring instrument to the sample. 785 nm laser spectrum Specifications Part Number HRAM Size Dimensions (mm 3 ) 125 x 223 x 85 Weight 2.4 kg (4.7lbs.) Excitation Source Wavelength 785 nm Bandwidth 0.2 nm typ./ 0.3 nm max. (FWHM) Output power mw Spectrograph Grating technology Transmission Volume Phase Grating (VPG ) Range (785nm) cm -1 Spectral resolution ~10-12 cm -1 Stray light rejection 0.05% fatty tissue (net) fatty tissue + background background Raman spectrum from fatty tissue in pork chop Detector Type TE cooled CCD Number of pixels 1024 Pixel size 14 µm Cooling temperature -20 C Integration time sec. Readout speed 250 khz Digitalized output 16-bit Dynamic range 3000:1 typ. Quantum Efficiency (max.) >90% Electronics Interface USB 2.0 Input power Battery operated Trigger mode 5 V TTL edge or level (optional) Battery life 4 hrs Software GUI Micro 20/20 for Windows XP/Vista SDK DLL, Sample Codes for VC and LabVIEW Spectral libraries User built or 3rd party (optional) 성경포토닉스 l 042)

8 Xantus TM -2 Raman Analyzer Portable Reliable Ruggedized Battery-operated Ideal for: Biomedical samples Algae Fluorescence suppression Narcotics & drugs Teaching labs Quality Control/Assurance The Xantus TM -2 is the only portable dispersive Raman spectrometer with 1064 nm excitation on the market today. It performs virtually fluorescence free Raman measurements with a powerful 1064 nm laser (>500 mw) and a thermoelectrically cooled 512 pixel InGaAs array detector (< -50 C). In addition to on-board data processing functions, the Xantus TM -2 can transmit data to centralized facilities via WiFi networks, thus enabling real time communication and decision making in mission critical situations. Tailored for Raman Each Xantus TM -2 is equipped with a 1064 nm laser stabilized for Raman spectroscopy. Output power: >500 mw. Linewidth: 0.2 nm FWHM typical. 10 s integration At the heart of every Xantus TM -2 is a transmissive VPG that delivers unsurpassed optical throughput. The spectrograph is customized to cover the cm -1 range which is the most chemically informative spectral region, thus leaving no pixels wasted. The detector is a 512 element InGaAs array thermoelectrically cooled to 50 C that drastically reduces dark currents and delivers superior signal to noise ratios. Xantus TM -2 is run by BaySpec s Micro 20/20 software that has an intuitive, streamlined user interface and can output data in.txt,.csv, or.spc format. Baseline correction function is intelligently built in, which greatly facilities reduction in fluorescence interference and account for drift in background Xantus TM -2 not only has no moving parts but also has its sensitive optical engine shock-resistant mounted, resulting in a ruggedized instrument that has withstood the rigors of field testing. 성경포토닉스 l 042)

9 Bring Instrumentation to the Sample Xantus TM -2 Raman Analyzer The Xantus TM -2 can perform measurements either via direct focusing or through a vial holder. For hard to reach spaces where a fiber collection bundle is more appropriate a fiber optic adapter is available. The Xantus TM -2 can handle samples in liquid, gel, powder, or solid forms, under laboratory conditions, or field environments, thus fulfilling the promise of bring instrument to the sample nm laser spectrum Specifications Raman Spectra of Starflower Oil nm 1064 nm 2500 Part Number Size HRAM Dimensions (mm 3 ) 140 x 218 x 410 Weight Excitation Source Wavelength Bandwidth Output power Spectrograph Grating technology < 7.3kg (15.9 lbs) 1064 nm 0.2 nm typ./ 0.3 nm max. (FWHM) mw Transmission Volume Phase Grating (VPG ) Range (785nm) cm -1 Spectral resolution ~12-15 cm -1 Stray light rejection 0.05% Detector Type TE cooled InGaAs Number of pixels 512 Pixel size 25 µm Cooling temperature -50 C Integration time Readout speed Digitalized output Dynamic range sec. 250 khz 16-bit 500:1 typ. Quantum Efficiency (max.) >90% Electronics Interface USB 2.0 Input power Trigger mode Battery life Software GUI Battery operated 5 V TTL edge or level (optional) 2 hrs Micro 20/20 for Windows XP/Vista Comparison of Raman spectra of SDK DLL, Sample Codes for VC and LabVIEW starflower oil taken with 785 nm and 1064 nm lasers, showing reduction in fluorescence. Spectral libraries User built or 3rd party (optional) 성경포토닉스 l 042)

10 Portability TM -1 Raman Analyzer Research-grade Transportable Battery-operated Ideal for: Polymorphs classification Incoming materials inspection Outgoing materials certification Geochemical applications Forensic laboratories Quality Control/Assurance Smaller than a typical lunch cooler and battery operated, the Portability TM Raman analyzer can be easily moved from lab to lab or transported to the field, yet under the hood it has a research grade Raman spectral engine. Aided by a 2048 element CCD array detector thermoelectrically cooled to less than 20 C, the Portability TM delivers full spectral coverage (300 to 3200 cm -1 ) with 5 cm -1 resolution. In addition to on board data processing functions, the Portability TM can transmit data to centralized facilities via WiFi networks, thus enabling real time communication and decision making in mission critical situations. Tailored for Raman The Portability TM -1 is equipped with a 785 nm laser stabilized for Raman spectroscopy. Output power: >500 mw. Linewidth: 0.2 nm FWHM typical. 1 s integration At the heart of the Portability TM -1 is a transmissive VPG that delivers unsurpassed optical throughput. The spectrograph offers full spectral coverage from 300 to 3200 cm -1. The detector is a 2048 pixel CCD array thermoelectrically cooled to 20 C that drastically reduces dark currents and delivers superior signal to noise ratios. The Portability TM -1 runs on BaySpec s Micro 20/20 software that has an intuitive, streamlined user interface and can output data in.txt,.csv, or.spc format. Baseline correction function is intelligently built in, which greatly facilities reduction in fluorescence interference and account for drift in background The Portability TM -1 not only has no moving parts but also has its sensitive optical engine shock-resistant mounted, resulting in a ruggedized instrument that has withstood the rigors of field testing. 성경포토닉스 l 042)

11 Bring Instrumentation to the Sample Portability TM -1 Raman Analyzer The Portability TM performs measurements via an active probe that can handle samples in liquid, gel, powder, or solid forms, under laboratory conditions, or field environments, thus fulfilling the promise of bring instrument to the sample. Active Probe Specifications Part Number PRAM Size Dimensions (mm 3 ) 240 x 260 x 135 Weight Excitation Source Wavelength < 4.5kg (9.9 lbs) 785 nm Bandwidth 0.2 nm typ./ 0.3 nm max. (FWHM) Output power mw Spectrograph Grating technology Transmission Volume Phase Grating (VPG ) Range (785nm) cm -1 EQUAL Spectral resolution ~5-7 cm -1 Stray light rejection 0.05% Detector Type TE cooled CCD Number of pixels 2048 Pixel size 14 µm Cooling temperature -20 C Integration time Readout speed Digitalized output sec. 250 khz 16-bit SUGAR Dynamic range 3000:1 typ. Quantum Efficiency (max.) >90% Electronics Interface Touch panel or USB 2.0 for external PC Input power Battery operated Trigger mode IN/OUT 5 V TTL edge or level Battery life 4 hrs Software BaySpec SDK Spectral libraries Micro 20/20 for Windows XP/Vista DLL, Sample Codes for VC and LabVIEW User built or 3rd Party (optional) 성경포토닉스 l 042)

12 RamSpec TM -1 Raman Analyzer High Performance Transportable Versatile Reliable Ideal for: Polymorphs classification Process monitoring Trace contamination identification Geochemical applications Forensic laboratories Chemical/Biotech research facilities The RamSpec TM -1 Raman analyzer, being the most compact instrument in its class, can be easily transported from one location to another, yet it is equipped with a high performance Raman spectral engine. Aided by a 2048 element CCD array detector thermoelectrically cooled to less than 20 C, the RamSpec TM -1 delivers full spectral coverage (300 to 3200 cm -1 ) with 5 cm -1 resolution. In addition to the standard 785 nm excitation laser wavelength, the RamSpec TM -1 can also be integrated with a 532 nm or 1064 nm excitation source, thus offering unprecedented flexibility and versatility in a laboratory instrument. Tailored for Raman The standard RamSpec TM -1 is equipped with a 785 nm laser stabilized for Raman spectroscopy. Output power: >500 mw. Linewidth: 0.1 nm FWHM typical. 532 nm and 1064 nm lasers are also available with the RamSpec TM -1 system. 1 s integration At the heart of the RamSpec TM -1 is a custom made transmissive VPG that delivers unsurpassed optical throughput. The spectrograph offers full spectral coverage from 300 to 3200 cm -1. The detector is a 2048 pixel CCD array for 532/785 nm excitation, which is thermoelectrically cooled to 20 C that drastically reduces dark currents and delivers superior signal to noise ratios. For 1064 nm excitation the detector is an InGaAs array thermoelectrically cooled to below 50 C, offering spectral coverage and signal to noise ratios previously only available from FT systems The RamSpec TM -1 runs on BaySpec s Spec 20/20 software that has an intuitive, streamlined user interface and can output data in.txt,.csv, or.spc format. Baseline correction function is intelligently built in, which greatly facilities reduction in fluorescence interference and account for drift in background. Additionally, Spec 20/20 offers a suite of other useful features such as peak identification and integration, overlay of spectra, and arithmetic operations. 성경포토닉스 l 042)

13 Bring Instrumentation to the Sample RamSpec TM -1 Raman Analyzer The RamSpec TM -1 performs measurements via a custom designed optical-fiber based Raman probe that can accommodate samples in liquid, gel, powder, or solid forms. The Raman probe can also be easily integrated into a larger system for automated process or environment monitoring. Raman Probe Specifications* Raman spectra of white and red wines taken with BaySpec s 1064 nm RamSpec TM -1 system Raman spectra of new and used engine oil taken with BaySpec s 1064 nm RamSpec TM -1 system Part Number Size Dimensions (mm 3 ) Weight Excitation Source Wavelength Bandwidth Output power Spectrograph Grating technology BRAM x315x84 4 kg (8.8 lbs) 785 nm 0.2 nm typ./ 0.3 nm max. (FWHM) mw Transmission Volume Phase Grating (VPG ) Range (785nm) cm -1 Spectral resolution ~5 cm -1 Stray light rejection 0.05% Detector Type TE cooled CCD Number of pixels 2048 Pixel size 14 µm Cooling temperature -20 C Integration time Readout speed Digitalized output Dynamic range sec. 250 khz 16-bit 3000:1 typ. Quantum Efficiency (max.) >90% Electronics Interface Input power Trigger mode Battery life Software BaySpec SDK Spectral libraries USB 2.0 for external PC 110 to 220 V AC IN/OUT 5 V TTL edge or level N/A Spec 20/20 for Windows XP/Vista DLL, Sample Codes for VC and LabVIEW User built or 3rd Party (optional) * Specifications are given for the 785 nm system. For 532 nm and 1064 nm laser sources please inquire with BaySpec. 성경포토닉스 l 042)

14 Customized Instruments With a dedicated and experienced team of instrumentation engineers and scientists, BaySpec has delivered over 15,000 spectrometers and spectrographs to leading research institutions around the world. Whether you need a unique laser wavelength or power, or a specific wavelength coverage and spectral resolution, or special mechanical dimensions, please contact us for a quote. We have the reputation for providing affordable, high-quality, customized spectroscopic solutions to our customers. OEM Partnership For the past decade BaySpec has been a leader in lowcost, high-volume manufacturing in the optical telecommunications industry and a reliable OEM partner for some of the Fortune 500 companies in the world. We understand what it takes to initiate and sustain a win-win partnership and thrive in an extremely competitive marketplace. Contact us with your OEM opportunities, whether it is a well established product or a brand new application, we are open-minded and thrive to create a long lasting and mutually beneficial relationship with you. 성경포토닉스 l 042)

15 Accessories for Raman Analyzers Item # Name Description Part Number: 1 Active Raman Probe Integrated Raman probe that provides excitation laser delivery, Raman scattering collection, laser line filtering, and electrical trigger in one ergonomically optimized package. Available for both 785 nm and 1064 nm excitation laser wavelengths. PROB-A 785 PROB-A Optical Raman Probe Integrated Raman probe that provide excitation laser delivery, Raman scattering collection, and laser line filtering in one compact package. Available for both 785 nm and 1064 nm excitation laser wavelengths. PROB-S-0785 PROB-S Sample Vial Holder Precision machined part that holds 10 mm diameter glass vials and fits all BaySpec Raman probes. Open slots also accommodate solid tablet samples mounted on business cards. RAHV 4 Solid Sampling Cone Precision machined cone that fits on all BaySpec Raman probes and minimizes random scattering from solid samples. RASC 5 Laser Shield Optical laser shield that provides at least 10-6 blocking efficiency at laser excitation wavelength and fits all BaySpec Raman probes. RALS-0785 RALS Ruggedized Carrying Case Case with pick and pluck foam. Air and water tight. Lifetime warranty. RACC 성경포토닉스 l 042)

16 Software: Micro 20/20 Intuitive Graphical User Interface (GUI): Important setup parameters, spectrum display, and real-time status indicators are all on a single, easy-to-see screen. Especailly designed for touch screen operations. Micro 20/20 GUI Pane 1: Spectrum window Pane 2: Data viewer Pane 3: Control panel Pane 2 Pane 1 Pane 3 Flexible, Multiple Data Formats: Can save and open data files in ASCII (.txt), Excel (.csv), or Grams/AI (.spc) to allow user the maximum flexibility in data storage and retrieval and interfacing to third party software packages. Automated Calibration Procedure: Built-in, automated calibration procedure leads users through easy-to-follow steps to accomplish Raman shift calibration in less than 30 seconds. 성경포토닉스 l 042)

17 Software: Micro 20/20 Intelligent Baseline Correction Function: Baseline constructed from multi-segment, smoothed polynomial allows effective removal of fluorescence background and slanted baseline with minimal artificial bias. Aspirin Raman Spectra with and without Baseline Correction Raw trace Baseline corrected Tailored Substance Libraries: With Micro 20/20 the user can quickly and easily build his/her own compound libraries to meet his/her specific identification needs, or can import libraries from third party vendors Powerful Substance Identification Functions: Whether the library is user built or imported from third parties, Micro 20/20 can identify unknown substances instantaneously and report quantitatively the match quality. It can even be trained to recognize mixtures. 성경포토닉스 l 042)

18 Applications of Raman Spectroscopy Raman spectroscopy is a non-destructive, no contact analytical technique that requires no or little sample preparation. With falling price barriers and reduction in physical dimensions its application areas are rapidly expanding, from lipid content determination in algae to crystal form identification in zeolites. Some of the most common application fields are high-lighted below. Chemical Biological Incoming/outgoing materials inspection and certification In situ, non-contact measurement of tissue samples, non-destructive, no labeling needed Online/at-line detector for Process Analytical Technology (PAT) Polymers: correlation of physical/chemical properties (molecular weight, viscosity, glass transition temperature, etc.) with Raman spectra Intracellular chemical mapping Lipid content quantitation in algae for biofuels Bacteria detection SERS for low level biological threat detection Petroleum product identification and analysis Identification of resins, petrochemicals, and commodity chemicals Pharmaceutical Drug polymorphs/solvates identification and classification Forensics Non-destructive and safe drug and narcotic identification / preservation of evidence Identification of explosives Trace forensic analysis of fibers, hair, pigments, ink, fabrics, etc. Identification of drug co-crystals Content analysis of tablets, gel caps, and liquids QA/QC of API, additives, and excipients Fast analytical tool for High Throughput Screening Toxic solvents identification Educational Geological Non-destructive identification of geological materials Authentification and anti-counterfeiting of gemstones Undergraduate/professional teaching labs Field expeditions Live demonstration in lecture halls Replacement for outdated bench-top instruments Origin identification of minerals and gemstones Evaluation of mining prospects 성경포토닉스 l 042)

19 Spectral quantity (a.u.) Applications of Raman Spectroscopy Challenges from the Real World Methanol content in gasoline quantified by Xantus TM -0 Panel below shows pure methanol, methanol -gasoline, and pure gasoline, respectively. Panel on the right shows the standard calibration curve Gasoline Methanol Correlation of spectral quantity with CH 3 OH percentage in gasoline y = x R² = % 20% 40% 60% 80% 100% Methanol (percentage) Raman Signal vs. Phosphate Concentration Phosphate mg/ml Ammonium dibasic phosphate granules Phosphate content in fertilizers determined by Xantus TM -1 Panel on the left shows the standard calibration curve Solutions for the Real World 성경포토닉스 l 042)

20 Raman Analyzer Selection Guide Model Xantus-0A Xantus-0B Xantus-1 Part Number HRAM-0A-0785 HRAM-0B-0785 HRAM Size Dimensions (mm 3 ) 85 x 190 x x 195 x x 223 x 85 Weight 1 kg (2.2 lbs.) 1.5 kg (3 lbs.) 2.4 kg (4.7lbs.) Excitation Source 532 nm Optional Optional 785 nm 1064 nm N/A N/A Laser bandwidth 0.2 nm typ./ 0.3 nm max. (FWHM) 0.2 nm typ./ 0.3 nm max. (FWHM) Output power ~80 mw mw Spectrograph Grating technology VPG VPG Range (785nm) cm cm -1 Spectral resolution ~15-17 cm -1 ~10-12 cm-1 Stray light rejection 0.05% 0.05% Detector Type CCD TE cooled CCD Number of pixels Pixel size 14 µm 14 µm Cooling temperature Ambient -20 C Integration time sec sec. Readout speed 250 khz 250 khz Digitalized output 16-bit 16-bit Dynamic range 2000:1 typ. 3000:1 typ. Quantum Efficiency (max.) >90% >90% Electronics Interface USB 2.0 USB 2.0 Input power Battery operated Battery operated Trigger mode 5 V TTL edge or level (optional) 5 V TTL edge or level (optional) Battery life 4 hrs 4 hrs Software GUI Micro 20/20 for Windows CE Micro 20/20 for Windows XP/Vista SDK DLL, Sample Codes for VC and LabVIEW DLL, Sample Codes for VC and LabVIEW 3rd party libraries N/A Optional 성경포토닉스 l 042)

21 Raman Analyzer Selection Guide Model Xantus-2 Portability-1 RamSpec-1* Part Number HRAM PRAM BRAM Size Dimensions (mm 3 ) 140 x 218 x x 260 x x315x84 Weight < 7.3kg (15.9 lbs) < 4.5kg (9.9 lbs) 4 kg (8.8 lbs) Excitation Source 532 nm N/A Optional Optional 785 nm N/A 1064 nm N/A Optional Laser bandwidth 0.2 nm typ./ 0.3 nm max. (FWHM) 0.2 nm typ./ 0.3 nm max. (FWHM) 0.1 nm typ./ 0.2 nm max. (FWHM) Output power mw mw mw Spectrograph Grating technology VPG VPG VPG Range (785nm) cm cm cm -1 Spectral resolution ~12-15 cm-1 ~5-7 cm-1 ~5 cm -1 Stray light rejection 0.05% 0.05% 0.05% Detector Type TE cooled InGaAs TE cooled CCD TE cooled CCD Number of pixels Pixel size 25 µm 14 µm 14 µm Cooling temperature -50 C -20 C -20 C Integration time sec sec sec. Readout speed 250 khz 250 khz 250 khz Digitalized output 16-bit 16-bit 16-bit Dynamic range 1000:1 typ. 3000:1 typ. 3000:1 typ. Quant. Eff. (max.) >90% >90% >90% Electronics Interface USB 2.0 Touch panel or USB 2.0 external PC USB 2.0 for external PC Input power Battery operated Battery operated 110 to 220 V AC Trigger mode 5 V TTL edge or level (optional) IN/OUT 5 V TTL edge or level IN/OUT 5 V TTL edge or level Battery life 2 hrs 4 hrs N/A Software GUI Micro 20/20 for Windows XP/Vista Micro 20/20 for Windows XP/Vista Spec 20/20 for Windows XP/Vista SDK DLL, Sample Codes for VC and LabVIEW DLL, Sample Codes for VC and LabVIEW DLL, Sample Codes for VC and LabVIEW 3rd party libraries Optional Optional Optional * Specifications are given for the 785 nm system. For 532 nm and 1064 nm laser sources please inquire with BaySpec. 성경포토닉스 l 042)