ASPECTS REGARDING THE IN VITRO PROPAGATION OF HIGHBUSH BLUEBERRY CULTIVAR BLUE CROP
|
|
- Myron Weaver
- 5 years ago
- Views:
Transcription
1 Bulletin UASVM, Horticulture 65(1)/8 pissn ; eissn ASPECTS REGARDING THE IN VITRO PROPAGATION OF HIGHBUSH BLUEBERRY CULTIVAR BLUE CROP Alexandru FIRA, Doina CLAPA, C. BADESCU Fruit Research Station Cluj, 5 Horticultorilor Street, 57, Cluj-Napoca, Romania, alexfiracluj@yahoo.com Keywords: blueberry, in vitro, Zeatin, -Isopentenyladenine, Thidiazuron, coconut water Abstract: The scope of this paper is to present the in vitro propagation of the highbush blueberry cultivar Blue Crop, a cultivar that has important difficulties regarding in vitro propagation as well as propagation by cuttings. As basal medium, Woody Plant Medium (WPM, after Lloyd & McCown) was used. As growth regulators, -Isopentenyladenine, Zeatin, Thidiazuron and raw coconut water obtained from ripe fruit were used. The iron source is also very important, FeNaEDDHA (Sequestrene 138) providing far better results than FeNaEDTA. FeNaEDDHA can either be added as iron supplement to the prepackaged media or it can be used as the only iron source in the media prepared from stock solutions of macroelements and microelements. The ph recommended for the media used for bluecrop in vitro propagation is 5. Also, some peculiarities of the in vitro propagation of cultivar Blue Crop are presented, in comparison with other cultivars. INTRODUCTION The blueberry cultivar Blue Crop was obtained in the USA by hybridizing the cultivars (Jersey x Pioneer) x (Stanley x June) and introduced into culture in 195. It is a vigorous, very productive cultivar, resistant to frost and drought, with very good quality fruit, easy to grow, but relatively difficult to propagate, the cuttings being dificult to root. In vitro propagation of cultivar Blue Crop also presents important difficulties, multiplication rate being relatively low. Its capacity for the in vitro neogenesis of shoots is also low. The highbush blueberry is propagated in vitro on an industrial scale in the USA as well as in some in vitro culture laboratories in Europe. Among the authors that have done research for the in vitro propagation of this species we cite: Chandler C.K., Draper A.D., Eccher T., Noe N., Piagnani C., Castelli S. etc. (1,, 3,, 5, 6, 7, 8, 9,1, 11, 1, 13). In the year, at the Fruit Research Station in Cluj, research was begun for the in vitro propagation of the highbush blueberry. MATERIALS AND METHODS In order to establish the optimal variant of media for this cultivar several experiments were done by using Woody Plant Medium as basal medium (after Lloyd and McCown), either original or modified and, as growth regulators, -Isopentenyladenine, Zeatin, Thidiazuron and coconut water deriving from ripe fruit were used at various concentrations (Table 1). Vitamin C (ascorbic acid) was used in powder form. The ph of the media was adjusted to 5. As gelling agents, Isubgol and agar were used. Stock-solutions of vitamins B1, B6 and nicotinic acid were used as well as stock solutions of hormones at. Myo-inositol was added to the media as powder. All the ingredients were added to the media before autoclavation, even 1
2 Vitamin C, a substance that easily decays at high temperatures. Coconut water was used at 1 ml/l of nutritive medium. Culture initiation was done in test tubes on WPM + 5 mg/l -Ip. For multiplication, Magenta GA7 polycarbonate vessels were used, with polypropylene caps. The cultures were incubated in the growth chamber in artificial light provided by fluorescent tubes. Light intensity was of about lux and temperature was of -6 C. For multiplication, cm long shoot fragments resulted from in vitro cultures were used as plant material. The cultures were watched for two months in the case of using Isubgol, for 7 weeks when using Zeatin and for three months in the experiments with Zeatin, coconut water and the ones with -Ip and vitamin C, because these variants provided slower growth. Table 1 Modified Woody Plant Medium, experimental variants Component WPM * salts Sequestrene 138 FeNaEDTA Myo-inositol Vitamin B 1 Vitamin B 6 Vitamin C Nicotinic acid Ip Zeatina TDZ Coconut water Agar Isubgol Sugar ph=5 *Woody Plant Medium Concentration Standard 1 mg/l 1 mg/l mg/l, 1, mg/l 5 mg/l, 3 mg/l.1,.,.5 mg/l 1 ml/l 5 g/l 15 g/l 3 g/l RESULTS AND DISCUSSIONS Cultivar Blue Crop is difficult to grow in vitro on the culture medium currently used (Table ) for the micropropagation of the highbush blueberry. On this medium short, thin and chlorotic shoots were obtained, and their bases get necrosed in 3-5 weeks after inoculation. Table Woody Plant Medium modified Component WPM salts FeNaEDTA Myo-inositol Vitamin B 1 Vitamin B 6 Nicotinic acid -Isopenteniladenine Sugar Agar ph=5 Concentration.3 g/l 1 mg/l mg/l 5 mg/l 3 g/l 5 g/l 15
3 As a measure of emergency the plants were passed onto a modified culture medium, in which agar was replaced with Isubgol and FeNaEDTA with Sequestrene 138 (Table 3). Variants of media with Sequestrene 138 and Isubgol for blueberry cultivar Blue Crop Table 3 Variants V V 1 V Basal medium+ growth regulators WPM* WPM* WPM* Iron supplement FeNaEDTA FeNaEDTA Sequestrene mg/l Gelling agent Agar 5 g/l Isubgol 15 g/l Isubgol 15 g/l *According to Table The multiplication rate of the blueberry on the agar-gelled medium was of 1.3 and for the two variants with Isubgol the multiplication rates were 3.9 for variant V1 and. for variant V (Figure 1) Multiplication rate in cultivar Blue Crop V V1 V Experimental variants Multiplication rate Multiplication rate in cultivar Blue Crop on media gelled with Isubgol Figure 1 The results demonstrate that for cultivar Blue Crop the product Isubgol used as a gelling agent is more adequate that the agar used initially, greatly increasing multiplication rate, shoot length and thus the quality of the plant material resulted from in vitro culture. The compound Sequestrene 138 also has a beneficial effect in the in vitro culture of the blueberry, the plants being greener, with a healthier appearance. Another measure taken for improving the in vitro propagation of cultivar Blue Crop was the replacement of -Ip with Zeatin. The results obtained are presented in Table 3 in comparison with other blueberry cultivars that were studied. Seven weeks after inoculation two vessels were taken randomly from each cultivar and the multiplication rate was the following (Table ): In cultivar Blue Crop the average number of shoots/plantlet was 7.3. Although the multiplication rate in cultivar Blue Crop (8.5) was lower in comparison with the other cultivars, it is significantly higher than on the best of the preceding variants (.) and the plantlets are more vigorous, do not get necrosed and can be successfully acclimatized. Another growth regulator tested for the in vitro propagation of cultivar Blue Crop was TDZ used in concentrations of.1,.,.5 and.. 16
4 Multiplication rate in cultivar Blue Crop on medium with Zeatin Cultivar Blue Crop Duke Elliott Hannah`s Choice No. of vessels used for multiplication No. of obtained vessels Multiplication rate Table It has been found that TDZ provides slow growth and relatively low multiplication rate, the resulting plants being deformed, with large leaves, with callus and compact masses of buds at the base of the stem. TDZ could be used for keeping cultivar Blue Crop in vitro for a long period of time. The average no. of shoots/plantlet obtained on the variants with TDZ in cultivar Blue Crop in comparison with other cultivars is presented in Table and Figure. As a growth regulator for the in vitro multiplication of cultivar Blue Crop raw coconut water was also used, in the concentration of 1 ml/l. On this medium the Blue Crop plantlets grew slowly and a small number of short shoots resulted. The average no. of shoots/plantlet is presented in Figure 3. under the influence of various concentrations of TDZ 8 6 Blue Crop Duke Elliott.. mg/l.5 mg/l resulted under the influence of coconut water Blue Crop Duke Elliott Average no. of shoots/plantl et Figure Figure 3 The effect of coconut water upon blueberry shoot proliferation Another aspect to be studied was the effect of vitamin C upon the in vitro multiplication of this blueberry cultivar. For this purpose, 3 variants of media were tested using the multiplication medium from Table 3 to which Vitamin C was added in 3 concentrations: V1- mg/l, V 1 mg/l and V3 mg/l. Vitamin C was added to the media before autoclavation. The reaction of cultivar Blue Crop on these media was compared with that of variants Duke and Elliott, and the results are presented in Table 5. We mention that in cultivar Blue Crop the largest number of shoots/vessel was obtained at the concentration of mg/l Vitamin C. The presents of Vitamin C also prevented the bases of the plantlets from blackening and the plants were relatively vigorous, suitable for acclimatization. By comparing the four variants tested for the in vitro multiplication of the highbush blueberry cultivar Blue Crop, respectively the variants with Zeatin, coconut water, TDZ and 17
5 -Ip + Vitamin C (Table 6 and Fig. ) there results that the best variant is the one with mg/l Zeatin. Table 5 The effect of Vitamin C upon blueberry in vitro multiplication Cultivar Variant Average no. of shoots/vessel Blue Crop Average no. of shoots/plantlet Duke Elliott obtained on the four variants tested Table 6 Variant V 1 Coconut water 1 ml/l 1.91 V Thidiazuron, mg/l 5.9 V 3 - -Ip (5 mg/l ) + vitamin C ( mg/l) 7.5 V - mg/l zeatin 7.3 Average number of shoots/plantlet in the different experimental variants Average no. of shoots/plantlet 1.91 V1 V V3 V Experimental variants Average number of shoots/plantlet in cultivar Blue Crop on various experimental variants Figure On the variant with Vitamin C almost the same number of shoots/plantlet were obtained, but the shoots are shorter, up to 3- cm as compared to the ones on Zeatin which have up to 7-8 cm, thus offering the largest multiplication rate. 18
6 CONCLUSIONS The micropropagation of the highbush blueberry cultivar Blue Crop is relatively difficult. The culture medium recommended for the micropropagation of cultivar Blue Crop is modified WPM (Table ) with maximum mg/l Zeatin as growth regulator. The use of Zeatin strongly stimulates shoot growth and considerably increases multiplication rate in the highbush blueberry. Culture on media with 5 mg/l -Ip and mg/l Vitamin C is also recommended, as it confers greater vigor to the shoots. It is not recommended to use TDZ as growth regulator, as it provides slower growth and lower multiplication rates, the resulting plants also being deformed. However, it is possible to maintain cultivar Blue Crop on the media with TDZ for long periods of time. It is also possible to use raw coconut water but a low number of shoots is obtained and the plantlets grow slowly. Isubgol can be successfully used as a gelling agent in blueberry in vitro culture, especially for cultivar Blue Crop, strongly stimulating plant growth. The compound Sequestrene 138 as iron source gives better results than FeNaEDTA in the blueberry in vitro cultures, conferring dark green colour to the plantlets. REFERENCES 1. Chandler, C. K., A. D. Draper, 1986, Effect of zeatin and ip on shoot proliferation of three blueberry clones in vitro, Hort Science 1: Da Silva, L. C., M. W Schuch, J. A. De Souza., A. C. Erig., L. E. C. Antunes, 6, Nutritive medium, growth regulators and cold in the in vitro establishment of blueberry (Vaccinium ashei Reade) Cv. Delite, R. Bras, Agrociência, Pelotas, v. 1, n., p Eccher, T., N. Noe, 1989, Comparison between ip and zeatin in the micropropagation of highbush blueberry (Vaccinium corymbosum), Acta Horticulturae. Fourth international symposium on Vaccinium culture, East Lansing, Michigan, USA, Aug. 1988, 1: Eccher, T., N. Noe, C. Piagnani, S. Castelli, 1986, Effects of Increasing Concentration of BAP and ip on in vitro culture of Vaccinium corymbosum, Acta Horticulturae 179, II : Gonzalez, M. V., M. Lopez, A. E. Valdes, R. J. Ordas,, Micropropagation of three berry fruit species using nodal segments from field-grow plants, Annals of Applied Biology, vol. 137, no.1, pp Gajdošová, A., M. G. Ostrolucká, G. Libiaková, E. Ondrušková, D. Šimala, 6, Microclonal propagation of Vaccinium sp. And Rubus sp. and detection of genetic variability in culture in vitro, Journal of Fruit and Ornamental Plant Research Vol. 1 (Suppl. 1). 7. George, E. F., , Plant Propagation by Tissue Culture, Part, Exegetics Limited, Edington, Wilts. 8. Jain, R., S. B. Babbar, 5. Guar gum and isubgol as cost-effective alternative gelling agents for in vitro multiplication of an orchid, Dendrobium chrysotoxum, Current Science, vol. 88, no., 5 January. 9. Krzewynska, D et al. Growth and yielding of highbush blueberry (Vaccinium corymbosum L. ) propagated by traditional and in vitro methods. Zeszyty naukowe Instytutu Sadownictwa I Kwiaciarstwa, Tom Marcotrigiano, M., S. P. McGlew, 1991, A Two-stage Micropropagation System for Cranberries, J. Amer. Soc. Hort. Sci. 116(5): Orlikowska, T., 1986, Micropropagation of Highbush Blueberry, Fruit Science Reports, Vol. XIII, No Ostrolucká M. G. et a.l, In vitro propagation of Vaccinium species, Acta Universitatis Latviensis, Biology, Vol pp Zimmerman, R. H., O. C. Broome, 198, Blueberry micropropagation, USDA-SEA Agr. Res. Results ARRNE. 11,
TISSUE CULTURE AND EX-VITRO ACCLIMATION OF RHODODENDRON sp.
Buletin USAMV-CN, 64/2007 (-) ISSN 1454-232 TISSUE CULTURE AND EX-VITRO ACCLIMATION OF RHODODENDRON sp. Clapa Doina, Al. Fira Fruit Research Station Cluj, 5 Horticultorilor Str. Horticultorilor nr.5, 400457
More informationMária Gabriela Ostrolucká 1, Gabriela Libiaková 1, Emília Ondrußková 2, Alena Gajdoßová 1
Acta Universitatis Latviensis, Biology, 2004, Vol. 676, pp. 207 212 In vitro propagation of Vaccinium species Mária Gabriela Ostrolucká 1, Gabriela Libiaková 1, Emília Ondrußková 2, Alena Gajdoßová 1 1
More informationIn Vitro Multiplication, Conservation and Ex Vitro Acclimation of Drosera rotundifolia
Bulletin UASVM Horticulture, 66(1)/2009 Print ISSN 1843-5254; Electronic ISSN 1843-5394 In Vitro Multiplication, Conservation and Ex Vitro Acclimation of Drosera rotundifolia Doina CLAPA 1), Alexandru
More informationEfficient micropropagation protocol for highbush blueberry (Vaccinium corymbosum L.) cv. Elliot
Romanian Biotechnological Letters Vol. 17, No. 1, 2012 Copyright 2012 University of Bucharest Printed in Romania. All rights reserved ORIGINAL PAPER Efficient micropropagation protocol for highbush blueberry
More informationIII. ÎNMULŢIRE, VIROLOGIE ŞI CULTURI DE ŢESUTURI PROPAGATION, VIROLOGY AND TISSUE CULTURE
III. ÎNMULŢIRE, VIROLOGIE ŞI CULTURI DE ŢESUTURI PROPAGATION, VIROLOGY AND TISSUE CULTURE MICROPROPAGAREA UNOR SOIURI DE MUR FARA SPINI THE MICROPROPAGATION OF SOME THORNLESS BLACKBERRY CULTIVARS Clapa
More informationINMULTIREA IN VITRO A PORTALTOIULUI DE CIRES GISELA 5 IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCK
INMULTIREA IN VITRO A PORTALTOIULUI DE CIRES GISELA 5 IN VITRO PROPAGATION OF GISELA 5 CHERRY ROOTSTOCK Doina Clapa 1, Alexandru Fira 1, Manuela Simu 1, Vlăduţ Codruţ Horga 2 1 Research Station for Fruit
More informationKeywords: Agarwood, satalum, resin, micropropagation, tok
Application of Tissue Culture Techniques in Woody Species Conservation, Improvement and Development in Vietnam: Agarwood (Aquilaria crassna Pierre ex LeComte) via tip Culture Tran Van Minh Institute of
More informationLow-Cost Alternatives for Conventional Tissue Culture Media
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 04 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.704.288
More informationAVOCADO CALLUS AND BUD CULTURE
Proc. Fla. State Hort. Soc. 96:181-182. 1983. AVOCADO CALLUS AND BUD CULTURE M. J. Young University of Florida, IF AS, Fruit Crops Department, Gainesville, FL 32611 Additional index words, tissue culture,
More informationA micropropagation system for Eucalyptus dunnii Eucalyptus sp
A micropropagation system for Eucalyptus dunnii Eucalyptus sp M. Fantini Jr., M.E. Cortezzi Graça To cite this version: M. Fantini Jr., M.E. Cortezzi Graça. A micropropagation system for Eucalyptus dunnii
More informationIn vitro regeneration capacity of the ornamental varieties related to the cultural media
In vitro regeneration capacity of the ornamental varieties related to the cultural media Elena Alina Rovină 1 *, Mirela Călinescu 1, Catiţa Plopa, 1 Valentina Isac 1 1 Research Institute for Fruit Growing
More informationIPC TECHNICAL PAPER SERIES NUMBER 264
THE INSTITUTE OF PAPER CHEMISTRY, APPLETON, WISCONSIN IPC TECHNICAL PAPER SERIES NUMBER 264 A TISSUE CULTURE SOLUTION TO A FORESTRY PROBLEM - PROPAGATION OF A TETRAPLOID EUROPEAN ASPEN THE S. R. WANN,
More informationPlantlet Regeneration via Somatic Embryogenesis in Four Species of Crocus
Plantlet Regeneration via Somatic Embryogenesis in Four Species of Crocus Roya Karamian Department of Biology, Faculty of Science Bu-Ali Sina University Hamadan Iran Keywords: meristems, micropropagation,
More informationSabri BRAHA, Petrit RAMA
Interaction ORIGINAL SCIENTIFIC between the PAPER time when taking hard wood cuttings and Indol Butyric Acid and Naphthalene Interaction between the time when taking hard wood cuttings and Indol Butyric
More informationMASS PRODUCTION OF A RARE AND ENDANGERED SPECIES, ASTRAGALUS MEMBRANACEUS VAR. ALPINUS NAKAI, THROUGH AXILLARY BUD CULTURE AND IN VIVO ROOTING TEST
MASS PRDUCTIN F A RARE AND ENDANGERED SPECIES, ASTRAGALUS MEMBRANACEUS VAR. ALPINUS NAKAI, THRUGH AXILLARY BUD CULTURE AND IN VIV RTING TEST Hyo-Jeong Kim, 1 Young-Je Kang, Seog-Gu Son 2, Hyung-Soon Choi,
More informationShoot Proliferation of Dendrobium Orchid with BAP and NAA
Journal of Biological Sciences 3 (): 058-062, 2003 ISSN 727-3048 2003 Asian Network for Scientific Information Shoot Proliferation of Dendrobium Orchid with BAP and NAA 2 S.K. Talukder, K.M. Nasiruddin,
More informationTitle: Development of Micropropagation and Acclimation Protocols for the Commercialization of a New Bonsai Ornamaental Tree for the California Market.
Title: Development of Micropropagation and Acclimation Protocols for the Commercialization of a New Bonsai Ornamaental Tree for the California Market. Authors: Dan E. Parfitt 1, Helen M. Chan 2, and Ali
More informationORGANOGENESIS IN CHRYSANTHEMUM MORIFOLIUM RAMAT (CULTIVAR ROMICA ) CALLUS CULTURES SMARANDA VÂNTU
Analele ştiinţifice ale Universităţii Al. I. Cuza Iaşi Tomul LII, s. II a. Biologie vegetală, 006 ORGANOGENESIS IN CHRYSANTHEMUM MORIFOLIUM RAMAT (CULTIVAR ROMICA ) CALLUS CULTURES SMARANDA VÂNTU Abstract:
More informationMICROPROPAGATION OF CHRYSANTHEMUM (CHRYSANTHEMUM MORIFOLIUM) USING SHOOT TIP AS EXPLANT
MICROPROPAGATION OF CHRYSANTHEMUM (CHRYSANTHEMUM MORIFOLIUM) USING SHOOT TIP AS EXPLANT R. Nalini Department of Biotechnology, Adhiparasakthi Agricultural College (Affiliated to TNAU) G. B. Nagar, Kalavai,
More informationEFFECT OF BENZYLAMINO PURINE AND NAPHTHALENE ACETIC ACID ON CALLUS AND PROTOCORM FORMATION OF DENDROBIUM CV. BANYAT PINK
Journal of Cell and Tissue Research Vol. 13(3) 3977-3981 (2013) (Available online at www.tcrjournals.com) ISSN: 0973-0028; E-ISSN: 0974-0910 Original Article EFFECT OF BENZYLAMINO PURINE AND NAPHTHALENE
More informationCHAPTER 2. IN VITRO REGENERATION OF Gerbera jamesonii Bolus Ex. Hook f. Previous research has proven that Gerbera jamesonii could successfully be
CHAPTER 2 IN VITRO REGENERATION OF Gerbera jamesonii Bolus Ex. Hook f. 2.1 EXPERIMENTAL AIMS Previous research has proven that Gerbera jamesonii could successfully be propagated in vitro. Different types
More informationTitle: Enhancement of Bramble Production in the Southeastern U.S. Through Micropropagation, Virus Indexing, and Field Evaluation for Trueness to Type
Title: Enhancement of Bramble Production in the Southeastern U.S. Through Micropropagation, Virus Indexing, and Field Evaluation for Trueness to Type Progress Report SRSFC Project #2006 01 Research Proposal
More informationInstitute of Plant Genetics and Biotechnology SAS, Akademická 2, P.O.Box 39A, Nitra, Slovak Republic;
IN VITRO PROPAGATION OF SEVERAL VACCINIUM CORYMBOSUM L. AND VACCINIUM VITIS-IDAEA L. CULTIVARS VAIRĀKU VACCINIUM CORYMBOSUM L. UN VACCINIUM VITIS-IDAEA L. ŠĖIRĥU IN VITRO PAVAIROŠANA Mária Gabriela Ostrolucká
More informationCOMPORTAREA UNOR SOIURI DE CAIS ÎN FAZA DE MULTIPLICARE IN VITRO BEHAVIOUR OF SOME APRICOT CULTIVARS DURING IN VITRO MULTIPLICATION PHASE
COMPORTAREA UNOR SOIURI DE CAIS ÎN FAZA DE MULTIPLICARE IN VITRO BEHAVIOUR OF SOME APRICOT CULTIVARS DURING IN VITRO MULTIPLICATION PHASE Mirela Călinescu 1, Maria Isac 1, Andi Ciobanu 2 1 Research Institute
More informationIn vitro Conservation of Rose Coloured Leadwort: Effect of Mannitol on Growth of Plantlets
Kasetsart J. (Nat. Sci.) 38 : 97-12 (24) In vitro Conservation of Rose Coloured Leadwort: Effect of Mannitol on Growth of Plantlets Rommanee Charoensub and Salak Phansiri ABSTRACT In vitro study of manitol
More informationAN ECONOMICAL AND EFFICIENT METHOD FOR MASS PROPAGATION OF IXORA COCCINEA
Pak. J. Bot., 36(4): 751-756, 2004. AN ECONOMICAL AND EFFICIENT METHOD FOR MASS PROPAGATION OF IXORA COCCINEA SAIFULLAH KHAN, MARIAM IFTIKHAR AND BUSHRA SAEED Plant Tissue Culture and Biotechnology Division,
More informationMICROPROPAGATION OF JATROPHA CURCAS (L.)
Indian J. Agric. Res., 43 (4) : 269-273, 2009 AGRICULTURAL RESEARCH COMMUNICATION CENTRE www.arccjournals.com / indianjournals.com MICROPROPAGATION OF JATROPHA CURCAS (L.) B.R. Ranwah, D.K. Gupta and M.A.
More informationEFFECT OF MEDIUM PH ON AXILLARY SHOOT PROLIFERATION
ACTA BIOLOGICA CRACOVIENSIA Series Botanica 52/2: 92 96, 2010 DOI: 10.2478/v10182-010-0029-1 EFFECT OF MEDIUM PH ON AXILLARY SHOOT PROLIFERATION OF SELECTED VACCINIUM VITIS-IDAEA L. CULTIVARS MÁRIA GABRIELA
More informationReceived : Accepted:
Ancient Science of Life Vol : XXVI (1) July, August, September 2004 Conservation of an endemic medicinal plant, Berberis tinctoria Lesch. In Nilgiris through micro propagation S.Paulsamy, S. Padmavathi
More informationH. E. Sommer, H. Y. Wetzstein and N. Lee
TISSUE CULTURE OF SWEETGUM (LIQUIDAMBAR STYRACIFLUA L.) H. E. Sommer, H. Y. Wetzstein and N. Lee Abstract.--An improved method for the tissue culture propagation of sweetgum (Liquidambar styraciflua L.)
More informationResearches regarding bitter melon (Momordica charantia) in vitro regeneration
Volume 14(3), 75-8, 21 JOURNAL of Horticulture, Forestry and Biotechnology Researches regarding bitter melon (Momordica charantia) in vitro regeneration Franţ Alexandra 1*, Botău Dorica 1 1 Banat s University
More informationMicro propagation of sugarcane (Saccharum officinarum L.) through auxiliary buds
Micro propagation of sugarcane (Saccharum officinarum L.) through auxiliary buds P S \Varakagoda, S Subasinghe, D L C Kumari and T S Neththikumara Department of Crop Science, Faculty of Agriculture, University
More informationREGENERATION SYSTEMS FOR PYRAMIDING DISEASE RESISTANCE INTO WALNUT ROOTSTOCKS
REGENERATION SYSTEMS FOR PYRAMIDING DISEASE RESISTANCE INTO WALNUT ROOTSTOCKS John E. Preece, Ana María Ibáñez, Quyen Tran, Dio Gunawan, Chuck Leslie, David Tricoli, and Abhaya Dandekar ABSTRACT The purpose
More informationScientific Papers of the R.I.F.G. Pitesti, Vol. XXVI, 2010
EVALUAREA AGROBIOLOGICĂ A SOIULUI DE AFIN DELICIA COMPARATIV CU SOIUL MARTOR BLUERAY AGROBIOLOGICAL ASSESSMENT OF THE HIGHBUSH BLUEBERRY DELICIA VAR. COMPARED WITH CONTROL BLUERAY VAR. Irina Ancu, Paulina
More informationAdult Plants and Juvenile Seedlings of Persimmon (Diospyros kaki L.)
J. Japan. Soc. Hort. Sci. 63(3) : 537-541. 1994. Comparison of Growth Rooting Characteristics of Micropropagated Adult Plants Juvenile Seedlings of Persimmon (Diospyros kaki L.) Ryutaro Tao, Jun Ito Akira
More informationOriginal Papers Plant Tissue Culture Letters, 11(3), (1994) INouE**, Koji NoMuRA***, Seiko TAKAHASHi**, OsHIMA* and Kiyoshi MASUDA**
Original Papers Plant Tissue Culture Letters, 11(3), 184-190 (1994) An Efficient Procedure for Plant Regeneration from Long-term-cultured Callus Tissue of Hybrid Poplar (Populus sieboldii Miq. X Populus
More informationINFLUENCE OF PLANT GROWTH REGULATORS ON IN VITRO CLONAL PROPAGATION OF DENDROBIUM SONIA EARSAKUL
INFLUENCE OF PLANT GROWTH REGULATORS ON IN VITRO CLONAL PROPAGATION OF DENDROBIUM SONIA EARSAKUL Priya Kumari, I.* Sabina George, T. & Rajmohan, K 1 Department of Pomology and Floriculture, College of
More informationFERN MULTIPLICATION KIT
FERN MULTIPLICATION KIT Product No. F34 India Contact 1 2 3 6 I 7...7 Product Number Kit Components Product Description 2 1 EA Box 1 Instruction Manual 1 C913/C21-10ea Culture Containers 1 F91-1ea Forceps,
More informationSOMATIC EMBRYOGENESIS OF DREPANOSTACHYUM FALCATUM AN IMPORTANT HILL BAMBOO-A RAPID MEANS OF MICROPROPAGATION
SOMATIC EMBRYOGENESIS OF DREPANOSTACHYUM FALCATUM AN IMPORTANT HILL BAMBOO-A RAPID MEANS OF MICROPROPAGATION I.D.Arya, R. Sharma & Sarita Arya Forest Genetics & Tree Propagation Division, Arid Forest Research
More informationEfficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk
Indian Journal of Biotechnology Vol 3, January 2004, pp. 113-118 Efficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk P Giridhar and G A Ravishankar*
More informationPlant Regeneration via Organogenesis and Somatic Embryogenesis
18 Plant Biotechnology: Practical Manual 2 Plant Regeneration via Organogenesis and Somatic Embryogenesis 2.1 BACKGROUND AND BASICS One can achieve plant regeneration in a test tube by using suitable culture
More informationSummary and conclusion
Summary and conclusion 6.1 Chemical sterilization of Explants Surface sterilization of explants collected from mature trees is necessary before initiation of any in vitro culture. In the present study
More informationDirect Regeneration of Shoot from Axillary Bud of Citrus Reticulate
Available online http://www.ijat-aatsea.com ISSN 1686-9141 Direct Regeneration of Shoot from Axillary Bud of Citrus Reticulate Shende, C. B. and Manik, S. R. Department of Botany Mohsinbhai Zaweri Arts,
More informationSeed Culture of Aromatic Rice Varieties Under Salt Stress
American Journal of Biology and Life Sciences 2015; 3(6): 260-264 Published online November 10, 2015 (http://www.openscienceonline.com/journal/ajbls) ISSN: 2381-3784 (Print); ISSN: 2381-3792 (Online) Seed
More informationMicropropagation of sugarcane (Saccharum spp.)
Plant Cell, Tissue and Organ Culture 10:47-55 (1987) Martinus Nijhoff Publishers, Dordrecht - Printed in the Netherlands 47 Short communication Micropropagation of sugarcane (Saccharum spp.) TSENG SHENG
More informationPlant regeneration of Anthurium andreanum cv Rubrun
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.7 No.3, Issue of December 15, 2004 2004 by Pontificia Universidad Católica de Valparaíso -- Chile Received June 4, 2004 / Accepted November 9, 2004
More informationPlant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids
Plant Cell, Tissue and Organ Culture 76: 11 15, 2004. 2003 Kluwer Academic Publishers. Printed in the Netherlands. 11 Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum
More informationIN VITRO GROWTH AND DEVELOPMENT OF DENDROBIUM HYBRID ORCHID. H. KHATUN 1, M. M. KHATUN 2, M. S. BISWAS 3 M. R. KABIR 4 AND M. AL-AMIN 5 Abstract
ISSN 0258-7122 Bangladesh J. Agril. Res. 35(3) : 507-514, September 2010 IN VITRO GROWTH AND DEVELOPMENT OF DENDROBIUM HYBRID ORCHID H. KHATUN 1, M. M. KHATUN 2, M. S. BISWAS 3 M. R. KABIR 4 AND M. AL-AMIN
More informationGregor Mendel Foundation Proceedings 2007:
Gregor Mendel Foundation Proceedings 2007: 54-61. Effect of the explants from different aged mother plant on callus induction and direct regeneration in jatropha(jatropha curcas L.) Pranesh K. J 1, Gururaja
More informationPlant Tissue Culture. Dr. Alain Lemansour UAE University Date Palm Development Research Unit Dept.
Plant Tissue Culture By Dr. Alain Lemansour UAE University Date Palm Development Research Unit Dept. What is it? Tissue culture is the term used for the process of growing cells artificially in the laboratory
More informationEffect of Cytokinins on Multiple Shoot Regeneration from Leaf Derived Callus of Inula
Plant Tissue Cult. & Biotech. 27(2): 189 194, 2017 (December) PTC&B Effect of Cytokinins on Multiple Shoot Regeneration from Leaf Derived Callus of Inula royleana DC. Samar Amin*, Zahoor A Kaloo and Seema
More informationQuestion Bank Vegetative Propagation
Question Bank Vegetative Propagation 1. Define vegetative reproduction. Ans. Vegetative reproduction is the formation of a new individual from any vegetative part of the plant body. 2. Mention any two
More informationMULTIPLE SHOOT REGENERATION IN DENDROBIUM FIMBRIATUM HOOK AN ORNAMENTAL ORCHID ABSTRACT
Kabir et al., The Journal of Animal & Plant Sciences, 23(4): 13, Page: J. 1140-1145 Anim. Plant Sci. 23(4):13 ISSN: 1018-7081 MULTIPLE SHOOT REGENERATION IN DENDROBIUM FIMBRIATUM HOOK AN ORNAMENTAL ORCHID
More informationMicropropagation of Salvia broussonetii Benth. - A Medicinal Plant Species
Plant Tissue Cult. & Biotech. 16(1): 19-23, 2006 (June) PTC&B Micropropagation of Salvia broussonetii Benth. - A Medicinal Plant Species S. Mederos-Molina * La Laguna (San Cristóbal de), 38200, Apartado
More informationUtilization of Embryogenic Cell Cultures for the Mass Production of Bulblets in Lilies
Utilization of Embryogenic Cell Cultures for the Mass Production of Bulblets in Lilies Sun Ki Kim and Byung Joon Ahn College of Bioresources Science Dankook University Cheonan 330-714 Korea Keywords: Oriental
More informationEffect of 6-Benzylaminopurine and -Naphthalene Acetic Acid on In vitro Production of MD2 Pineapple Planting Materials
World Applied Sciences Journal 3 (4): 64-69, 2008 ISSN 88-4952 IDOSI Publications, 2008 Effect of 6-Benzylaminopurine and -Naphthalene Acetic Acid on In vitro Production of MD2 Pineapple Planting Materials
More informationEffects of Different Concentrations of α-naphthaleneacetic Acid and 6-benzylaminopurine on Shoot Regeneration of Vinca minor L.
J. Agric. Sci. Technol. (2008) Vol. 10: 337-344 Effects of Different Concentrations of α-naphthaleneacetic Acid and 6-benzylaminopurine on Shoot Regeneration of Vinca minor L. F. Raouf Fard 1, A. Moieni
More informationStudies on Vegetative Propagation ~.f Tulips Regeneration of Bulblets in Bulb Scale Segments Culturt. 1 in vitro
J. Japan. Soc. Hort. Sci. 49(2) : 235-24u. ' QR0. Studies on Vegetative Propagation ~.f Tulips IV. Regeneration of Bulblets in Bulb Scale Segments Culturt. 1 in vitro Yoshlo NISHIUCHI Hokkaido University
More informationHow Important Are Bacteria for the Vase Life of Cut Gerbera Flowers?
How Important Are Bacteria for the Vase Life of Cut Gerbera Flowers? U. van Meeteren and R. Schouten Wageningen University Horticultural Supply Chains Group PO Box 630, 6700 AP Wageningen The Netherlands
More informationMICROPROPAGATION OF RICE (ORYZA SATIVA L. CV SWAT-II) THROUGH SOMATIC EMBRYOGENESIS
Pak. J. Bot., 37(2): 237-242, 2005. MICROPROPAGATION OF RICE (ORYZA SATIVA L. CV SWAT-II) THROUGH SOMATIC EMBRYOGENESIS IHSAN ILAHI, SHAZIA BANO, MUSARRAT JABEEN AND FAZAL RAHIM Department of Botany, University
More informationMicropropagation of GF-677 rootstocks (Prunus amygdalus x P. persica)
Micropropagation of GF-677 rootstocks (Prunus amygdalus x P. persica) Kamali K., Majidi E., Zarghami R. in Ak B.E. (ed.). XI GREMPA Seminar on Pistachios and Almonds Zaragoza : CIHEAM Cahiers Options Méditerranéennes;
More informationThe Influence of Plant Density and Shoot Pruning on Yield of Bell Pepper Cultivated in Plastic Tunnel
Bulletin UASVM Horticulture, 67(1/2010 Print ISSN 1843-5254; Electronic ISSN 1843-5394 The Influence of Plant Density and Shoot Pruning on of Bell Pepper Cultivated in Plastic Tunnel Danut MANIUTIU, Rodica
More informationIn vitro propagation of Musa sp (Banana)
ISSN: 2319-7706 Volume 3 Number 7 (2014) pp. 399-404 http://www.ijcmas.com Original Research Article In vitro propagation of Musa sp (Banana) M.Anbazhagan *, B. Balachandran and K. Arumugam Department
More informationTISSUE CULTURE II. Organogenesis. PlSc 300 LAB Learn tissue culture techniques that promote organ formation.
76 TISSUE CULTURE II Organogenesis PlSc 300 LAB 11 REFERENCE: Text: 663 666; 706 712; 717 718. OBJECTIVES: 1. Learn tissue culture techniques that promote organ formation. 2. Practice making leaf and shoot
More informationThe influence of food colorants on the growth and development of in vitro plantlets belonging to different Romanian varieties
Volume 21(1), 122-126, 2017 JOURNAL of Horticulture, Forestry and Biotechnology www.journal-hfb.usab-tm.ro The influence of food colorants on the growth and development of in vitro plantlets belonging
More informationTHE INITIATION OF LOTUS (Nelumbo nucifera) VITROCULTURE
Analele Univ. Oradea, Fasc. Biologie, Tom. XII, 25 pp.185-19 THE INITIATION OF LOTUS (Nelumbo nucifera) VITROCULTURE 1 PhD students, University from Oradea Carmen-Lumini a CHIRIIAC & Julieta-Emilia ROMOCEA
More informationTHE EFFECT OF DIFFERENT PLANT GROWTH REGULATORS ON SHOOT INDUCTION OF Orthosiphon aristatus Boldingh.
THE EFFECT OF DIFFERENT PLANT GROWTH REGULATORS ON SHOOT INDUCTION OF Orthosiphon aristatus Boldingh. R. Zaidah & M.S. Nazri Biology Department, Faculty of Science, UTM, 81310 UTM Skudai, Johor, Malaysia.
More informationField Performance of Grafted Fruit-Tree Rootstocks Was Not Affected by Micropropagation
Field Performance of Grafted Fruit-Tree Rootstocks Was Not Affected by Micropropagation J.A. Marín, M. Castillo, E. García and P. Andreu Estación Experimental de Aula Dei (CSIC). Apartado 202, 50080 Zaragoza
More informationMICROPHOPAGATION OF DATE PALM (PHOENIX TISSUE CULTURE TECHNIQUE
Annals of Arid Zone 28 (1&2): 137-141, 1989 MICROPHOPAGATION OF DATE PALM (PHOENIX DACTYLIFERA L.) CV KHADRA WY USING TISSUE CULTURE TECHNIQUE N.L. KACKAR, K.R. SOLANKI AND S.P. JOSHI Central Arid Zone
More informationInternational Journal of Pharma and Bio Sciences DIRECT REGENERATION FROM EMBRYO CULTURES OF LYCOPERSICON ESCULENTUM MILL CV PUSA RUBY ABSTRACT
Research Article Plant science International Journal of Pharma and Bio Sciences ISSN 0975-6299 DIRECT REGENERATION FROM EMBRYO CULTURES OF LYCOPERSICON ESCULENTUM MILL CV PUSA RUBY P. KARUNAKAR RAO Department
More informationProlonging the Vase Life of Cut Carnation L. cv. Reina by Using Different Preservative Solutions
Bulletin UASVM Horticulture, 67(1)/2010 Print ISSN 1843-5254; Electronic ISSN 1843-5394 Prolonging the Vase Life of Cut Carnation L. cv. Reina by Using Different Preservative Solutions Orsolya FEIGEL-TEREK
More informationStudies on the influence of genotype and culture media composition on growing explants in the in vitro initiation phase
Volume 15(2), 221-225, 2011 JOURNAL of Horticulture, Forestry and Biotechnology Studies on the influence of genotype and culture media composition on growing explants in the in vitro initiation phase Tudor
More informationRegulation of cytokinin response-competence by cold treatment of mature Pinus sylvestris tissues in vitro
Acta Universitatis Latviensis, Biology, 2004, Vol. 676, pp. 143 148 Regulation of cytokinin response-competence by cold treatment of mature Pinus sylvestris tissues in vitro Una Andersone 1 *, Gederts
More informationAFRICAN VIOLET MULTIPLICATION KIT
AFRICAN VIOLET MULTIPLICATION KIT Product No. A137 India Contact Revised May 2013 Page 1 TABLES OF CONTENTS KIT COMPONENTS... 2 MATERIALS REQUIRED BUT NOT PROVIDED... 2 INTRODUCTION... 3 STERILIZATION
More informationUse Rooting Hormones, or Not? Multiple Applications May Be Best
Presented at the Eastern Region meeting of the International Plant Propagator s Society, September 2017 Use Rooting Hormones, or Not? Multiple Applications May Be Best Joel Kroin Hortus USA Corp. support@hortus.com
More informationMicropropagation of Sterile and Non-Flowering Nicotiana Lines
2008 The Japan Mendel Society Cytologia 73(1): 9 13, 2008 Micropropagation of Sterile and Non-Flowering Nicotiana Lines Sarala K.*, Rao R. V. S., Murthy T. G. K. and Satyavani J. V. R. Central Tobacco
More informationIn Vitro Flowering and Shoot Multiplication from Nodal Explants of Ceropegia bulbosa Roxb. var. bulbosa
Taiwania, 48(2): 106-111 In Vitro Flowering and Shoot Multiplication from Nodal Explants of Ceropegia bulbosa Roxb. var. bulbosa S. John Britto (1, 2), E. Natarajan (1) and D. I. Arockiasamy (1) (Manuscript
More informationMicropropagation Scheme of Curcuma alismatifolia Gagnep.
Micropropagation Scheme of Curcuma alismatifolia Gagnep. N. Toppoonyanont, S. Chongsang, S. Chujan, S. Somsueb and P. Nuamjaroen Department of Biology, Faculty of Science, Maejo University Chiang Mai 50290
More informationSK. Jaffar * et al. /International Journal Of Pharmacy&Technology
ISSN: 0975-766X CODEN: IJPTFI Available Online through Research Article www.ijptonline.com INVITRO REGENERATION OF BANANA (MUSA SPP.) M. Guru Prasad 1, SK. Jaffar* 2, Ch. Manjula 3 Regional Agriculture
More informationIN VITRO PROPAGATION OF THE BALKAN ENDEMIC SPECIES VERBASCUM ERIOPHORUM GODR. Abstract. Introduction
767 Bulgarian Journal of Agricultural Science, 22 (No 5) 2016, 767 771 Agricultural Academy IN VITRO PROPAGATION OF THE BALKAN ENDEMIC SPECIES VERBASCUM ERIOPHORUM GODR. Zh. P. YORDANOVA *, M. A. ROGOVA
More informationIN VITRO SHOOT MULTIPLICATION AND CALLUS INDUCTION IN GLADIOLUS HYBRIDUS HORT.
Pak. J. Bot., 40(2): 517-522, 2008. IN VITRO SHOOT MULTIPLICATION AND CALLUS INDUCTION IN GLADIOLUS HYBRIDUS HORT. FAHEEM AFTAB, MEMOONA ALAM AND HUMERA AFRASIAB Department of Botany, University of the
More informationESTABLISHMENT AND CLONAL PROPAGATION OF IN VITRO PLANTLETS OF LEPTOSPERMUM SCOPARIUM
ESTABLISHMENT AND CLONAL PROPAGATION OF IN VITRO PLANTLETS OF LEPTOSPERMUM SCOPARIUM R.H. BRAUN & D.W.M. LEUNG Department of Plant and Microbial Sciences, University of Canterbury, Private Bag, New Zealand.
More informationThidiazuron in the Improvement of Banana Micropropagation
Thidiazuron in the Improvement of Banana Micropropagation Sin-Wan Lee Taiwan Banana Research Institute, P.O. Box 18, Chiuju, Pingtung Taiwan 904, R.O.C. Keywords: Paclobutrazol Abstract In 1983 a commercial
More informationIn vitro Micropropagation of Syngonium podophyllum
Available online at www.ijpab.com ISSN: 2320 7051 Int. J. Pure App. Biosci. 2 (4): 88-92 (2014) INTERNATIONAL JOURNAL OF PURE & APPLIED BIOSCIENCE Research Article In vitro Micropropagation of Syngonium
More informationFull length Article Studies on callus induction and shoot regeneration in Tomato
Science Research Reporter, 4(1): 89-93, (April - 2014) RUT Printer and Publisher (http://jsrr.net) ISSN: 2249-2321 (Print); ISSN: 2249-7846 (Online) Received: 15-01-2014, Revised: 15-02-2014, Accepted:
More informationEnvironmental and Genotypic Effects on the Growth Rate. of in Vitro Cassava Plantlet
1 Environmental and Genotypic Effects on the Growth Rate of in Vitro Cassava Plantlet By Sunday E. Aladele National Centre for Genetic Resources and Biotechnology, Ibadan, Nigeria Abstract Two cassava
More informationMICROCLONAL PROPAGATION BLACK CURRANT
UDC 581.143.6+634.723.1 2016 T. Medvedeva, Candidate of Biological Sciences N. Triapitsyna, T. Natalchuk, Candidates of Agricultural Sciences Ya. Zapolskyi Institute of Horticulture of NAAS MICROCLONAL
More informationEffect of different levels of sucrose on microtuberization and different substrates on minituber production resulted from potato meristem culture
IOSR Journal of Agriculture and Veterinary Science (IOSR-JAVS) e-issn: 2319-2380, p-issn: 2319-2372. Volume 4, Issue 6 (Sep. - Oct. 2013), PP 58-62 Effect of different levels of sucrose on microtuberization
More informationCallus induction and somatic embryogenesis of Phalaenopsis
Plant Cell Reports (1998) 17: 446 450 Springer-Verlag 1998 Y. Ishii T. Takamura M. Goi M. Tanaka Callus induction and somatic embryogenesis of Phalaenopsis Received: 11 June 1997 / Revision received: 6
More informationIn vitro regeneration performance of Corchorus olitorius
J. Bangladesh Agril. Univ. 8(1): 1 6, 2010 ISSN 1810-3030 In vitro regeneration performance of Corchorus olitorius M. Hoque 1, K. M Nasiruddin 2, G. K. M. N. Haque 3 and G. C. Biswas 4 1 Dept. of Agronomy
More informationEffect of BA and 2iP on Shoot Proliferation and Somaclonal Variation of Gardenia jasminoides Ellis in vitro Culture
R ESEARCH ARTICLE ScienceAsia 27 (2001) : 137-141 Effect of BA and 2iP on Shoot Proliferation and Somaclonal Variation of Gardenia jasminoides Ellis in vitro Culture Ngarmnij Chuenboonngarm a,b, Suvimon
More informationIn Vitro Multiplication Of Date Palm. Iqbal Hussain, Hamid Rashid, Aish Muhammad & Azra Quraishi
In Vitro Multiplication Of Date Palm Iqbal Hussain, Hamid Rashid, Aish Muhammad & Azra Quraishi Agricultural Biotechnology Institute, National Agriculture Research Center, Islamabad, Pakistan. ABSTRACT
More informationAn efficient protocol for clonal micropropagation of Mentha piperita L. (Pipperment)
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (4):518523 ISSN : 22497412 CODEN (USA): AJPSKY An efficient protocol for clonal micropropagation
More informationSelection of mother plant. Preparation of explants. Fresh inoculation. Multiplication. Rooting. Planting out. Primary hardening. Secondary hardening
7.6 Protocol for micropropagation of banana 153 PROTOCOL FOR MICROPROPAGATION OF BANANA Joy P. P., Anjana R. & Prince Jose, Pineapple Research Station (Kerala Agricultural University), Vazhakulam-686670,
More informationVirus and bacteria elimination from in vitro sweetpotato plants. International Potato Center (CIP)
Virus and bacteria elimination from in vitro sweetpotato plants International Potato Center (CIP) Pathogen elimination: Contribute to the safe conservation of germplasm Allow the production of high quality
More informationEffect of Genotype, Explant Type and Culture Medium on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro
435 Bulgarian Journal of Agricultural Science, 12 (2006), 435-439 National Centre for Agrarian Sciences Effect of Genotype, Explant Type and Culture Medium on Shoot Regeneration in Tomato (Lycopersicon
More informationPRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHOD
J. Plant Develop. 20(2013): 57 61 PRELIMINARY CONSERVATION EFFORT ON RHIZOPHORA ANNAMALAYANA KATHIR., THE ONLY ENDEMIC MANGROVE TO INDIA, THROUGH IN VITRO METHOD KATHIRESAN Kandasamy 1, RAVINDER Singh
More information2. What are the advantages and disadvantages of plant tissue culture?
UNIT VI - PLANT TECHNOLOGIES Lesson 2: Plant Tissue Culture Competency/Objective: Explain the process of tissue culture. Study Questions References 1. What is plant tissue culture? 2. What are the advantages
More informationThe preferred carbohydrate in plant cell culture media is sucrose. Glucose and fructose may be substituted in some cases, glucose being as
Introduction One of the most important factors governing the growth and morphogenesis of plant tissues in culture is the composition of the culture medium. The basic nutrient requirements of cultured plant
More informationCascade huckleberry, mountain
Shoot Regeneration and Plantlet Formation by Cascade Huckleberry, Mountain Huckleberry, and Oval-leaf Bilberry on a Zeatin-containing Nutrient Medium Samir C. Debnath 1,3 and Danny L. Barney 2 ADDITIONAL
More information