DRAFT MALAYSIAN STANDARD OFFICER/SUPPORT STAFF: (NAD/) Descriptors: tissue culture, planting material, banana

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1 DRAFT MALAYSIAN STANDARD 15A003R0 STAGE: (40.20) DATE: 01/12/ /01/2016 Banana tissue culture planting material - Specification OFFICER/SUPPORT STAFF: (NAD/) ICS: Descriptors: tissue culture, planting material, banana Copyright DEPARTMENT OF STANDARDS MALAYSIA

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3 CONTENTS Page Committee representation.... ii Foreword... iii 1 Scope Normative references Terms and definitions Basic requirements Planting material requirements Outsourced plantlets requirements Plant handling Inspection Compliance... 4 Annex A Morphology of banana tissue culture planting material... 5 Annex B Process flow of mother plant inspection process... 6 Annex C Process flow for the micropropagation and certification of banana planting material. 7 Annex D Mother plant... 8 Annex E Sucker... 9 Annex F Banana plantlets Annex G Age comparison of banana tissue culture planting material Bibliography STANDARDS MALAYSIA 2015 All right reserved i

4 Committee representation The Industry Standards Committee on Agriculture (ISC A) under whose authority this Malaysian Standard was developed, comprises representatives from the following organisations: Department of Agriculture Malaysia Department of Standards Malaysia Department of Veterinary Services Federal Agricultural Marketing Authority Forest Research Institute Malaysia Malaysia Fruit Exporters Association Malaysian Association of Standards Users Malaysian Palm Oil Association Malaysian Rubber Board Ministry of Agriculture and Agro-based Industry Ministry of Plantation Industries and Commodities Ministry of Science, Technology and Innovation SIRIM Berhad (Secretariat) Universiti Kebangsaan Malaysia The Technical Committee on Planting Materials which supervised the development of this Malaysian Standard consists of representatives from the following organisations: Department of Agriculture Malaysia Malaysian Agricultural Research and Development Institute Malaysian Pineapple Industry Board Malaysian Rubber Board National Seed Association Malaysia Seng Kee Fruit Nursery Sin Seng Huat Seeds Sdn Bhd SIRIM Berhad (Secretariat) Universiti Kebangsaan Malaysia Universiti Putra Malaysia The Working Group on Planting Materials - Banana Tissue Culture which developed this Malaysian Standard consists of representatives from the following organisations: Arus Primajaya Sdn Bhd Department of Agriculture Malaysia Malaysian Agricultural Research and Development Institute SIRIM Berhad (Secretariat) Universiti Putra Malaysia Co-opted member: Department of Agriculture Malaysia ii STANDARDS MALAYSIA 2015 All right reserved

5 Foreword This Malaysian Standard was developed by the Working Group on planting materials - Banana tissue culture under the authority of the Industry Standards Committee on Agriculture. Compliance with a Malaysian Standard does not of itself confer immunity from legal obligations. STANDARDS MALAYSIA 2015 All right reserved iii

6 1 Scope Banana tissue culture planting material - Specification This Malaysian Standard specifies quality and inspection requirements for the production of banana planting material (plantlet) propagated through tissue culture techniques. 2 Normative references The following normative references are indispensable for the application of this standard. For dated references, only the edition cited applies. For undated references, the latest edition of the normative reference (including any amendments) applies. Manual Skim Pengesahan Bahan Tanaman (SPBT), Jabatan Pertanian Malaysia. 3 Terms and definitions For the purposes of this standard, the following definitions apply. 3.1 aseptic Microbial-free. 3.2 mother plant / ortet Plants used for the propagation of certified banana planting materials. 3.3 explant Plant parts used for initiating cultures. 3.5 culture An entity comprising the container, growth media and propagules. 3.8 plantlet A young plant used as planting material. 3.9 rogue plant A plant of the same species but not of the clone being propagated or having undesirable variation from the standard plant height The height is measured from the collar to the tip of the pseudostem / trunk. Refer Annex A mother plant plot An area planted with mother plants of approved sources for the propagation of planting materials. STANDARDS MALAYSIA 2015 All right reserved 1

7 3.13 sword sucker A vigorous sucker that develops from the rhizome and usually emerges close to the mother plant, characterised by narrow leaves and a large rhizome, used for replanting and tissue culture propagation off-types Plants which do not conform to the varietal description offered by the breeder or the organisation which releases the variety certifying agency An agency appointed by an authoritative body for certifying mother plants and planting materials nursery A place where plants are propagated and grown to usable size with suitable necessities variety A plant grouping within a single botanical taxon of the lowest known rank, defined by the reproducible expression of its distinguishable morphological characteristics and genetic identity micropropagation A practice of rapid multiplication of stock plant material to produce a large number of progeny plants through plant tissue culture techniques tissue culture A collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition, widely used to produce clones of a plant. 4 Basic requirements 4.1 Mother plant Mother plants can be sourced from clonally or conventionally propagated plant population as recommended in the Manual Skim Pengesahan Bahan Tanaman (SPBT) Mother plants shall be true-to-type, healthy and free from diseases and pests. Refer Annex D Mother plant plot shall be free of rogue plant A minimum of one inspection shall be made at the time of fruit maturity Mother plants certified by the certifying agency shall meet all of the above criteria. 2 STANDARDS MALAYSIA 2015 All right reserved

8 4.2 Suckers Only sword suckers are used as the source of explant. Water suckers are not suitable as source of explants. Refer Annex E The sucker shall be collected from certified mother plants. 4.3 Micropropagation requirements All micropropagation and laboratories facilities shall have minimum requirements to carry out aseptic manipulations and to provide clean area for culture growth and storage Hygienic conditions should be maintained strictly during micropropagation The process flow for the micropropagation and certification of banana planting material is as per Annex C. 4.4 Nursery The nursery requirements for the production of planting material are as follows: a) Effective sanitation practices including pest and disease monitoring and prevention shall be adhered to. b) Varieties must be physically separated and properly labeled to prevent varietal mixture. Each plot shall be clearly labeled (legible and indelible) with its plot number, type of variety, number of plantlet and planting date. c) No conventionally propagated banana planting material shall be grown in the same nursery. d) A minimum of one inspection shall be made prior to sale. 5 Planting material requirements 5.1 Banana tissue culture planting material requirements shall be as given in Table 1. Table 1. Specification for banana tissue culture planting material Factor Specification Number of leaf (minimum) 5 Plant height in cm (minimum) 15 Age of plantlet (week) Refer Annex G 6 to 12 Polybag size (inch) 5 x 7 STANDARDS MALAYSIA 2015 All right reserved 3

9 5.2 In the nursery lot, plantlets not conforming to requirements specified in Table 1 shall not exceed 5.0% by number in total. 5.3 Plantlets shall be free from any visual symptoms of disease, physical damages and abnormalities such as variegated and necrotic leaf. Refer Annex F. 5.4 Off-types and plantlets infected by disease or infested by pests shall be treated or discarded under the supervision of the certifying agency as recommended in Manual Skim Pengesahan Bahan Tanaman (SPBT). The maximum permissible requirements for certification are as shown in Table 2. Table 2. Maximum permissible requirements for plantlet certification Factor Maximum permitted level (%) Off-types 0.1 Disease and pest Plantlets conforming to requirements in Table 1 and Table 2 may be certified by the certifying agency and are deemed fit for sale. 6 Outsourced plantlets requirement Outsourced plantlets obtained for the production of planting material shall have a certificate of assurance along with other related documents. 7 Plantlet handling Prior to transportation, plantlets shall undergo a hardening process for specific durations. Plantlet handling shall be carried out properly to reduce evapotranspiration, root disturbances and other injuries. 8 Inspection Inspection of all plantlets shall be carried out by inspectors appointed by the certifying agency in accordance to procedures of the agency. 9 Compliance All plantlet consignment certified by the certifying agency at the time of inspection, with respect to clonal fidelity, cultivation, trueness-to-type, health and vigor conditions, shall be deemed to comply with this Standard. 4 STANDARDS MALAYSIA 2015 All right reserved

10 Annex A (informative) Morphology of a banana tissue culture planting material/plantlet Pseudostem Leaf Plant height STANDARDS MALAYSIA 2015 All right reserved 5

11 Annex B (informative) Process flow of mother plant inspection General survey of the mother plant plot and the surrounding area Determining the starting point (first mother plant to be inspected) Inspect for trueness to type, pest infestation and disease infection for every plant, and work row by row Pass Plant certified as mother plant Fail Plant not certified as mother plant 6 STANDARDS MALAYSIA 2015 All right reserved

12 Annex C (informative) Process flow for the micropropagation and certification of banana planting material Suckers obtained from certified mother plant Treatment of suckers Culture initiation in the production laboratory Multiplication of shoots Rooting of shoots Acclimatisation and transfer of plantlets into polybags in the nursery Hardening of plantlets Inspection of planting material for certification (6-12 weeks of age) Fail Planting material not certified Pass Planting material certified and ready for sale STANDARDS MALAYSIA 2015 All right reserved 7

13 D.1 Healthy mother plant D.2 Diseased mother plant Annex D (informative) Mother plant 8 STANDARDS MALAYSIA 2015 All right reserved

14 Annex E (informative) E.1 Sword sucker E.2 Water sucker Sucker STANDARDS MALAYSIA 2015 All right reserved 9

15 Annex F (informative) Banana plantlets F.1 Healthy plantlet which fulfills the requirements F.2 Reject plantlet diseased F.3 Reject plantlet - insect damage 10 STANDARDS MALAYSIA 2015 All right reserved

16 F.4 Reject plantlet - stunted growth F.5 Reject plantlet - physical damage F.6 Reject plantlet - variegated leaf (off-type) F.7 Reject plantlet - necrotic leaf STANDARDS MALAYSIA 2015 All right reserved 11

17 Annex G (informative) Age comparison of banana tissue culture planting material 6-week old plantlets 12-week old plantlets 12 STANDARDS MALAYSIA 2015 All right reserved

18 Bibliography [1] Prosedur Proses Utama Proses Pengeluaran Bahan Tanaman Pisang Secara Kultur Tisu, MARDI, (2014) [2] National Certification System for Tissue Culture Raised Plant (NCS-TCP), Department of Biotechnology Ministry of Science & Technology Government of India, (2013) STANDARDS MALAYSIA 2015 All right reserved 13

19 Acknowledgements Members of Technical Committee on Planting Materials Dr Izham Ahmad (Chairman) Ms Nor Azian Duriat (Secretary) Mr Mohd Anim Hosnan Ms Sudarti Asri Mr Mohd Shaib Jaafar Mr Soo Kok Keong Mr Freddie Soh Tan Seng Prof Dr Normah Mohd. Noor Assoc Prof Dr Uma Rani Sinniah Universiti Putra Malaysia SIRIM Berhad Department of Agriculture Department of Agriculture (Crop Quality Control Division) Malaysian Agricultural Research and Development Institute (Seed Unit) Seng Kee Fruit Nursery Sin Seng Huat Seeds Sdn Bhd Universiti Kebangsaan Malaysia Universiti Putra Malaysia Members of Working Group on Planting Materials - Banana Tissue Culture Mr Mohd Shaib Jaafar (Chairman) Ms Nor Azian Duriat (Secretary) Mr Tan Chun Chuang Mr Mohd Anim Hosnan Ms Halimah Hashim Co-opted member: Mr Zulmi Yaacob Malaysian Agricultural Research and Development Institute (Seed Unit) SIRIM Berhad Arus Primajaya Sdn Bhd Department of Agriculture (Paddy Industrial Crops and Floriculture Division) Malaysian Agricultural Research and Development Institute Department of Agriculture (Crop Quality Control Division)

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