Plantlet Regeneration via Somatic Embryogenesis in Four Species of Crocus
|
|
- Angelina Henderson
- 6 years ago
- Views:
Transcription
1 Plantlet Regeneration via Somatic Embryogenesis in Four Species of Crocus Roya Karamian Department of Biology, Faculty of Science Bu-Ali Sina University Hamadan Iran Keywords: meristems, micropropagation, plant growth regulators, somatic embryos Abstract Somatic embryogenesis was initiated in four species of Crocus, C. sativus, C. cancellatus, C. michelsonii and C. caspius using shoot meristem culture on LS medium containing 4 mg/l NAA and 4 mg/l BA or 1 mg/l 2,4-D and 4 mg/l kinetin. Somatic embryogenesis was asynchronous in all of four species and various stages of somatic embryo development were observed when embryogenic calli with globular somatic embryos were transferred into half strength MS medium containing 1 mg/l abscisic acid. Maturated embryos could be germinated on half strength MS medium supplemented with 2 mg/l GA. Complete plantlets were obtained by transferring of germinated embryos into half strength MS medium supplemented with 1 mg/l NAA and 1 mg/l BA at 2 ºC under a 16/ h (light/dark) cycle. INTRODUCTION Crocus is an important genus belonging to Iridaceae. It has nine species in Iran, many of which are valuable and horticulturally important. Like other ornamental monocotyledonous species with corms, they are generally propagated vegetatively. Numerous studies have appeared regarding in vitro propagation of Crocus sativus (George et al., 1992; Ebrahimzadeh et al., 1996; Ebrahimzadeh et al. 2) but other members of the genus have received little attention (Ebrahimzadeh et al., 1996). The present study reports how regeneration of plantlets was achieved via somatic embryogenesis from shoot meristems culture in C. sativus, C. cancellatus, C. michelsonii and C. caspius. MATERIALS AND METHODS Corms were collected from different areas of Iran during two annual flowering seasons. Sprouted corms were utilized as source of explants. Bulblets with small portion of corm attached were separated and washed with tap water and surface sterilized in.1% HgCl 2 solution for 1 min followed by rinsing times with sterile distilled water. Shoot meristems were dissected and cultured on LS medium (Linsmaier and Skoog, 196) containing % sucrose and different concentration of cytokinins (BA, Kn) and auxins (2,4-D, IAA, NAA) (Tables 1-). All media were solidified by.7% agar after adjusting the ph to. and autoclaved for 1 min at 121 C at 14 kpa. Cultures were incubated in dark at 2 C. The experiment was repeated twice using 2 explants per treatment. The data for callus initiation were scored after 6 weeks of culture. Callus and embryogenic callus induction frequencies were calculated as the percent of cultured shoot tips producing callus and embryogenic callus respectively. Data from a total of two replications were statistically analyzed using a SAS program and separated by DMRT. Embryogenic nature of cultures was maintained by visual identification and selection of embryogenic sectors and removal of soft and translucent nonembryogenic portions at the time of subculturing. Embryogenic calli with globular embryos were transferred to a halfstrength liquid MS medium (Murashige and Skoog, 1962) without growth regulator or with 1 mg/l ABA for maturation of somatic embryos. Maturated somatic embryos were transferred to half strength MS medium containing 2 mg/l GA for germination. For plant regeneration, germinated somatic embryos were transferred to half strength MS Proc. I st IS on Saffron Eds: J.-A. Fernández & F. Abdullaev Acta Hort 6, ISHS 24 2
2 medium supplemented with 1 mg/l NAA and 1 mg/l BA and incubated under a 16/ h (light/dark) photoperiod with a temperature of 2 C. RESULTS AND DISCUSSION After 6- weeks of culture, shoot meristems on LS medium containing different growth regulators formed calli. Frequency of callus initiation, embryogenic callus production and morphogenetic nature of callus varied with kind of growth regulator used (Tables 1-). In the studied species, the highest frequency of callus initiation and embryogenic callus induction were recorded on LS medium with NAA and BA or 2,4-D and kin. During initial stages of callus development yellowish and soft calli grew from cultured explants, which after - transfers were visually distinguishable to be of two types: yellow to brownish friable and nodular embryogenic calli with shining globular structures and off-white to yellow soft calli (Figures 1-4). Production of embryogenic calli increased considerably with careful selection of embryogenic regions and frequent subculturing on the maintenance MS medium. Somatic embryo development proceeded through various stages of globular, heart-shaped and bipolar embryos were observed in half strength liquid MS basal medium growth regulator free or with 1 mg/l abscisic acid. Somatic embryos showing bipolarity could be germinated on half strength MS medium containing 2 mg/l GA. Germinated somatic embryos transferred into half strength MS medium containing 1 mg/l of each NAA and BA resulted in shoot and root development and plantlet regeneration after -1 weeks of inoculation under a 16/ h (light/dark) photoperiod with a temperature of 2 ºC (Figures 1-4). Some plantlets were left to grow in culture under the same conditions without further subcultures produced corm. Then regenerated plantlets were kept on a medium with low nutrient levels to harden them before transfer to pots. The results reported here showed that two types of calli grew from cultured shoot meristems. Production of embryogenic callus was initially slow but careful selection of embryogenic regions and frequent subculturing on the maintenance MS medium resulted in vigorous proliferation of embryogenic callus. Somatic embryo development in all of studied species was asynchronous and different stages could be observed simultaneously in the same embryogenic callus. This protocol agrees with general mode of plant regeneration through somatic embryogenesis in number of plants. It was observed that 2ºC temperature was suitable for the regeneration of calli. This result agrees with earlier reports on this genus (Ebrahimzadeh et al., 2). Many aspects can affect the maturation and germination process, such as temperature and light condition, age of explants and concentration of growth regulators (Firoozabady and DeBoer, 199). The present study where callus cultures derived from meristems, facilitates the proliferation of embryogenic callus and subsequent regeneration of plantlets from somatic embryos and proved to be a useful alternate method for micropropagation. Literature Cited Ebrahimzadeh, H., Karamian, R. and Noori-Daloii, M.R Regeneration of neoformant organs from organ parts of some species of Crocus. J. Sci. I. R. Iran 7: Ebrahimzadeh, H., Karamian, R. and Noori-Daloii, M.R. 2. Somatic embryogenesis and plantlet regeneration in saffron, Crocus sativus L. J. Sci. I. R. Iran 11: Firoozabady, E. and DeBoer, D.L Plant regeneration via somatic embryogenesis in many cultivars of cotton (Gossypium hirsutum L.). In Vitro Cell. Dev. Biol. 29P: George, P.S., Visvanath, S., Ravishankar, G.A. and Ventkataraman, L.V Tissue culture of saffron (Crocus sativus L.): Somatic embryogenesis and shoot regeneration. Food Biotechnol. 6: Linsmaier, E.M. and Skoog, F.196. Organic growth factor requirements of tobacco tissue cultures. Physiol. Plant. 1: Murashige, T. and Skoog, F A revised medium for rapid growth and bioassays 24
3 with tobacco tissue cultures. Physiol. Plant. 1: Tables Table1. Morphogenic response of shoot meristem culture of C. sativus Growth regulators Auxin + Cytokinin BA + NAA BA + 2,4-D No. of Responsive explants % Callus Initiation 7.a 6ab 6b bc 2.d 27.de % Embryogenic callus initiation 4a 2.ab ab 2.ab 27.b b Morphogenic nature Compact,nodular embryogenic Yellowish soft friable and compact nodular BA + IAA 7.e 12.e 7.e Very slow growing compact callus with growing buds Kn + NAA Kn + 2,4-D Kn + IAA d 6b 4cd 2.bc 47.c 2de 2de 2de 1de 2 b 17.c 1c 2bc 2bc 22.bc 1c 12.c 1c Compact hard callus with green buds Brownish soft friable and compact nodular Whitish hard callus with growing buds Explants were cultured on LS medium supplemented with growth regulators in different combinations. Value is the mean for two experiments, 2 explants in each experiment. Data were taken 6 weeks after cultures. Mean separation within rows by DMRT, p.. 2
4 Table 2. Morphogenic response of shoot meristem culture of C. cancellatus Growth regulators Auxin + Cytokinin 2,4-D + BA No. of Responsive explants 6 4 % Callus initiation e 2 de 2e % Embryogenic callus initiation c c c Morphogenetic nature Yellow to brownish, compact, nodular IAA + BA e 1 de e c Off-white to yellow, compact, nodular NAA + BA a 6 ab bc 1 1 c Yellowish, soft callus 2,4-D + Kn ab 6 abc 4d 4 ab 4 a b Off-white, friable, nodular embryogenic callus IAA + Kn 4 2 de 2e 1 de 1 c 1 c White, soft callus with leaf-like structures NAA + Kn White, soft callus 2 de 4d 2 de Explants were cultured on LS medium supplemented with growth regulators in different combinations. Value is the mean for two experiments, 2 explants in each experiment. Data were taken 6 weeks after cultures. Mean separation within rows by DMRT, p.. 26
5 Table. Morphogenic response of shoot meristem culture of C. michelsonii Growth regulators Auxin + Cytokinin BA + NAA BA + 2,4-D No. of Responsive explants % Callus Initiation b c 7. b 2 b 1 b b % Embryogenic callus initiation. cd c Morphogenetic nature Off-white, soft callus Brownish, compact, nodular callus BA + IAA Kn + NAA ab 4 ab 4 ab 2 b 16.7 bc Off-white, soft, friable, nodular callus Kn + 2,4-D a ab 62. a 27 b 4 a 2 b Yellow to brownish, friable, nodular embryogenic callus Kn + IAA c Brownish, soft callus Explants were cultured on LS medium supplemented with growth regulators in different combinations. Value is the mean for two experiments, 2 explants in each experiment. Data were taken 6 weeks after cultures. Mean separation within rows by DMRT, p.. 27
6 Fig. 1. Plantlet regeneration via somatic embryogenesis in C. sativus. Fig. 2. Plantlet regeneration via somatic embryogenesis in C. cancellatus 2
7 . Fig.. Plantlet regeneration via somatic embryogenesis in C. michelsonii. Fig. 4. Plantlet regeneration via somatic embryogenesis in C. caspicus. 29
SOMATIC EMBRYOGENESIS AND REGENERATION OF PLANTLET IN SAFFRON, CROCUS SATIVUS L.
J. Sci. I. R. Iran Vol. 11, No. 3, Summer 2000 SOMATIC EMBRYOGENESIS AND REGENERATION OF PLANTLET IN SAFFRON, CROCUS SATIVUS L. H. Ebrahimzadeh 1*, R. Karamian 2 and M. R. Noori-Daloii 3 1 Department of
More informationIn Vitro Microcorm Formation in Saffron (Crocus sativus L.)
In Vitro Microcorm Formation in Saffron (Crocus sativus L.) W. Raja, G. Zaffer, S.A. Wani Division of Plant Breeding & Genetics Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir
More informationMICROPROPAGATION OF RICE (ORYZA SATIVA L. CV SWAT-II) THROUGH SOMATIC EMBRYOGENESIS
Pak. J. Bot., 37(2): 237-242, 2005. MICROPROPAGATION OF RICE (ORYZA SATIVA L. CV SWAT-II) THROUGH SOMATIC EMBRYOGENESIS IHSAN ILAHI, SHAZIA BANO, MUSARRAT JABEEN AND FAZAL RAHIM Department of Botany, University
More informationSOMATIC EMBRYOGENESIS OF DREPANOSTACHYUM FALCATUM AN IMPORTANT HILL BAMBOO-A RAPID MEANS OF MICROPROPAGATION
SOMATIC EMBRYOGENESIS OF DREPANOSTACHYUM FALCATUM AN IMPORTANT HILL BAMBOO-A RAPID MEANS OF MICROPROPAGATION I.D.Arya, R. Sharma & Sarita Arya Forest Genetics & Tree Propagation Division, Arid Forest Research
More informationEffect of Cytokinins on Multiple Shoot Regeneration from Leaf Derived Callus of Inula
Plant Tissue Cult. & Biotech. 27(2): 189 194, 2017 (December) PTC&B Effect of Cytokinins on Multiple Shoot Regeneration from Leaf Derived Callus of Inula royleana DC. Samar Amin*, Zahoor A Kaloo and Seema
More informationSummary and conclusion
Summary and conclusion 6.1 Chemical sterilization of Explants Surface sterilization of explants collected from mature trees is necessary before initiation of any in vitro culture. In the present study
More informationKeywords: Agarwood, satalum, resin, micropropagation, tok
Application of Tissue Culture Techniques in Woody Species Conservation, Improvement and Development in Vietnam: Agarwood (Aquilaria crassna Pierre ex LeComte) via tip Culture Tran Van Minh Institute of
More informationPlant Regeneration via Organogenesis and Somatic Embryogenesis
18 Plant Biotechnology: Practical Manual 2 Plant Regeneration via Organogenesis and Somatic Embryogenesis 2.1 BACKGROUND AND BASICS One can achieve plant regeneration in a test tube by using suitable culture
More informationMICROPHOPAGATION OF DATE PALM (PHOENIX TISSUE CULTURE TECHNIQUE
Annals of Arid Zone 28 (1&2): 137-141, 1989 MICROPHOPAGATION OF DATE PALM (PHOENIX DACTYLIFERA L.) CV KHADRA WY USING TISSUE CULTURE TECHNIQUE N.L. KACKAR, K.R. SOLANKI AND S.P. JOSHI Central Arid Zone
More informationIn vitro propagation of Musa sp (Banana)
ISSN: 2319-7706 Volume 3 Number 7 (2014) pp. 399-404 http://www.ijcmas.com Original Research Article In vitro propagation of Musa sp (Banana) M.Anbazhagan *, B. Balachandran and K. Arumugam Department
More informationInternational Journal of Pharma and Bio Sciences DIRECT REGENERATION FROM EMBRYO CULTURES OF LYCOPERSICON ESCULENTUM MILL CV PUSA RUBY ABSTRACT
Research Article Plant science International Journal of Pharma and Bio Sciences ISSN 0975-6299 DIRECT REGENERATION FROM EMBRYO CULTURES OF LYCOPERSICON ESCULENTUM MILL CV PUSA RUBY P. KARUNAKAR RAO Department
More informationInternational Journal of Pharma and Bio Sciences RAPID IN VITRO PROPAGATION TECHNIQUE FOR SUGARCANE VARIETY 018
International Journal of Pharma and Bio Sciences RESEARCH ARTICLE BIOTECHNOLOGY RAPID IN VITRO PROPAGATION TECHNIQUE FOR SUGARCANE VARIETY 018 SATPAL SINGH BISHT*, AJIT KUMAR ROUTRAY & ROJITA MISHRA Department
More informationIN VITRO SHOOT MULTIPLICATION AND CALLUS INDUCTION IN GLADIOLUS HYBRIDUS HORT.
Pak. J. Bot., 40(2): 517-522, 2008. IN VITRO SHOOT MULTIPLICATION AND CALLUS INDUCTION IN GLADIOLUS HYBRIDUS HORT. FAHEEM AFTAB, MEMOONA ALAM AND HUMERA AFRASIAB Department of Botany, University of the
More informationCHAPTER 2. IN VITRO REGENERATION OF Gerbera jamesonii Bolus Ex. Hook f. Previous research has proven that Gerbera jamesonii could successfully be
CHAPTER 2 IN VITRO REGENERATION OF Gerbera jamesonii Bolus Ex. Hook f. 2.1 EXPERIMENTAL AIMS Previous research has proven that Gerbera jamesonii could successfully be propagated in vitro. Different types
More informationGregor Mendel Foundation Proceedings 2007:
Gregor Mendel Foundation Proceedings 2007: 54-61. Effect of the explants from different aged mother plant on callus induction and direct regeneration in jatropha(jatropha curcas L.) Pranesh K. J 1, Gururaja
More informationIn vitro Conservation of Rose Coloured Leadwort: Effect of Mannitol on Growth of Plantlets
Kasetsart J. (Nat. Sci.) 38 : 97-12 (24) In vitro Conservation of Rose Coloured Leadwort: Effect of Mannitol on Growth of Plantlets Rommanee Charoensub and Salak Phansiri ABSTRACT In vitro study of manitol
More informationAn efficient protocol for somatic embryogenesis of garlic (Allium sativum L.) using root tip as explant
J. Bangladesh Agril. Univ. 12(1): 1 6, 2014 ISSN 1810-3030 An efficient protocol for somatic embryogenesis of garlic (Allium sativum L.) using root tip as explant M. N. Hassan 1*, M. S. Haque 2, M. M.
More informationInvestigation of Plant Growth Regulators Effects on Callus Induction and Shoot Regeneration of Bunium persicum (Boiss.) B. Fedtsch.
J. Agr. Sci. Tech. (2009) Vol. 11: 481-486 Investigation of Plant Growth Regulators Effects on Callus Induction and Shoot Regeneration of Bunium persicum (Boiss.) B. Fedtsch. M. Valizadeh 1, and S. K.
More informationUtilization of Embryogenic Cell Cultures for the Mass Production of Bulblets in Lilies
Utilization of Embryogenic Cell Cultures for the Mass Production of Bulblets in Lilies Sun Ki Kim and Byung Joon Ahn College of Bioresources Science Dankook University Cheonan 330-714 Korea Keywords: Oriental
More informationChapter 4. In vitro callus multiplication, regeneration and microcorm induction in Amorphophallus paeoniifolius
Chapter 4 70 Chapter 4 In vitro callus multiplication, regeneration and microcorm induction in Amorphophallus paeoniifolius 4.1 Introduction The high incidence of mosaic disease, corm dormancy and non-availability
More informationEFFECT OF BENZYLAMINO PURINE AND NAPHTHALENE ACETIC ACID ON CALLUS AND PROTOCORM FORMATION OF DENDROBIUM CV. BANYAT PINK
Journal of Cell and Tissue Research Vol. 13(3) 3977-3981 (2013) (Available online at www.tcrjournals.com) ISSN: 0973-0028; E-ISSN: 0974-0910 Original Article EFFECT OF BENZYLAMINO PURINE AND NAPHTHALENE
More informationIN VITRO GROWTH AND DEVELOPMENT OF DENDROBIUM HYBRID ORCHID. H. KHATUN 1, M. M. KHATUN 2, M. S. BISWAS 3 M. R. KABIR 4 AND M. AL-AMIN 5 Abstract
ISSN 0258-7122 Bangladesh J. Agril. Res. 35(3) : 507-514, September 2010 IN VITRO GROWTH AND DEVELOPMENT OF DENDROBIUM HYBRID ORCHID H. KHATUN 1, M. M. KHATUN 2, M. S. BISWAS 3 M. R. KABIR 4 AND M. AL-AMIN
More informationAlbino Regenerants Proliferation of Dendrocalamus Asper In vitro
World Journal of Agricultural Sciences 10 (1): 09-13, 2014 ISSN 1817-3047 IDOSI Publications, 2014 DOI: 10.5829/idosi.wjas.2014.10.1.1755 Albino Regenerants Proliferation of Dendrocalamus Asper In vitro
More informationThe effects of BA and NAA on multiplication of Butterwort (Pinguicula gigantea) in vitro
Journal of Agricultural Technology 2011 Vol. 7(5): 1349-1354 Journal of Agricultural Available Technology online http://www.ijat-aatsea.com 2011, Vol.7(5): 1349-1354 ISSN 1686-9141 The effects of BA and
More informationORGANOGENESIS IN CHRYSANTHEMUM MORIFOLIUM RAMAT (CULTIVAR ROMICA ) CALLUS CULTURES SMARANDA VÂNTU
Analele ştiinţifice ale Universităţii Al. I. Cuza Iaşi Tomul LII, s. II a. Biologie vegetală, 006 ORGANOGENESIS IN CHRYSANTHEMUM MORIFOLIUM RAMAT (CULTIVAR ROMICA ) CALLUS CULTURES SMARANDA VÂNTU Abstract:
More informationIN VITRO PROPAGATION OF EGYPTIAN DATE PALM: 11-DIRECT AND INDIRECT SHOOT PROLIFERATION FROM SHOOT-TIP EXPLANTS OF Phoenix dactylifera L. CV. ZAGHLOOL.
IN VITRO PROPAGATION OF EGYPTIAN DATE PALM: 11-DIRECT AND INDIRECT SHOOT PROLIFERATION FROM SHOOT-TIP EXPLANTS OF Phoenix dactylifera L. CV. ZAGHLOOL. Shawky A. Bekheet and Mahmoud M. Saker Plant Cell
More informationAlbino Regenerants Proliferation of Dendrocalamus asper in vitro
Int.J.Curr.Microbiol.App.Sci (218) Special Issue-7: 527-533 International Journal of Current Microbiology and Applied Sciences ISSN: 2319-776 Special Issue-7 pp. 527-533 Journal homepage: http://www.ijcmas.com
More informationMICROPROPAGATION OF JATROPHA CURCAS (L.)
Indian J. Agric. Res., 43 (4) : 269-273, 2009 AGRICULTURAL RESEARCH COMMUNICATION CENTRE www.arccjournals.com / indianjournals.com MICROPROPAGATION OF JATROPHA CURCAS (L.) B.R. Ranwah, D.K. Gupta and M.A.
More informationIn vitro regeneration performance of Corchorus olitorius
J. Bangladesh Agril. Univ. 8(1): 1 6, 2010 ISSN 1810-3030 In vitro regeneration performance of Corchorus olitorius M. Hoque 1, K. M Nasiruddin 2, G. K. M. N. Haque 3 and G. C. Biswas 4 1 Dept. of Agronomy
More informationEFFECT OF DIFFERENT CULTURAL CONDITIONS ON MICROPROPAGATION OF ROSE (ROSA INDICA L.)
Pak. J. Bot., 41(6): 2877-2882, 2009. EFFECT OF DIFFERENT CULTURAL CONDITIONS ON MICROPROPAGATION OF ROSE (ROSA INDICA L.) ASAD SHABBIR 1, NOSHEEN HAMEED 1 AMIR ALI 2 AND RUKHSANA BAJWA 1 1 Institute of
More informationEffect of BA NAA and 2,4-D on Micropropagation of Jiaogulan (Gynostemma pentaphyllum Makino)
2012 International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies. International Transaction Journal of Engineering, Management, & Applied Sciences & Technologies http://tuengr.com,
More informationINFLUENCE OF PLANT GROWTH REGULATORS ON IN VITRO CLONAL PROPAGATION OF DENDROBIUM SONIA EARSAKUL
INFLUENCE OF PLANT GROWTH REGULATORS ON IN VITRO CLONAL PROPAGATION OF DENDROBIUM SONIA EARSAKUL Priya Kumari, I.* Sabina George, T. & Rajmohan, K 1 Department of Pomology and Floriculture, College of
More informationOriginal Papers Plant Tissue Culture Letters, 11(3), (1994) INouE**, Koji NoMuRA***, Seiko TAKAHASHi**, OsHIMA* and Kiyoshi MASUDA**
Original Papers Plant Tissue Culture Letters, 11(3), 184-190 (1994) An Efficient Procedure for Plant Regeneration from Long-term-cultured Callus Tissue of Hybrid Poplar (Populus sieboldii Miq. X Populus
More informationMicropropagation of Salvia broussonetii Benth. - A Medicinal Plant Species
Plant Tissue Cult. & Biotech. 16(1): 19-23, 2006 (June) PTC&B Micropropagation of Salvia broussonetii Benth. - A Medicinal Plant Species S. Mederos-Molina * La Laguna (San Cristóbal de), 38200, Apartado
More informationMicro propagation of sugarcane (Saccharum officinarum L.) through auxiliary buds
Micro propagation of sugarcane (Saccharum officinarum L.) through auxiliary buds P S \Varakagoda, S Subasinghe, D L C Kumari and T S Neththikumara Department of Crop Science, Faculty of Agriculture, University
More informationSeed Culture of Aromatic Rice Varieties Under Salt Stress
American Journal of Biology and Life Sciences 2015; 3(6): 260-264 Published online November 10, 2015 (http://www.openscienceonline.com/journal/ajbls) ISSN: 2381-3784 (Print); ISSN: 2381-3792 (Online) Seed
More informationRapid Micropropagation and Callus Induction of Catharanthus roseus in Vitro Using Different Explants
World Journal of Agricultural Sciences 7 (6): 699-704, 2011 ISSN 1817-3047 IDOSI Publications, 2011 Rapid Micropropagation and Callus Induction of Catharanthus roseus in Vitro Using Different Explants
More informationPlant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids
Plant Cell, Tissue and Organ Culture 76: 11 15, 2004. 2003 Kluwer Academic Publishers. Printed in the Netherlands. 11 Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum
More informationIN VITRO SHOOT TIP CULTURE OF COTTON (GOSSYPIUM HIRSUTUM)
Pak. J. Bot., 36(4): 817-823, 2004. IN VITRO SHOOT TIP CULTURE OF COTTON (GOSSYPIUM HIRSUTUM) BUSHRA RASHID *, TAYYAB HUSNAIN AND SHEIKH RIAZUDDIN Centre for Applied Molecular Biology, 87-West Canal Bank
More informationIn vitro REGENERATION OF MUNGBEAN (Vigna radiata L.) FROM DIFFERENT EXPLANTS
Progress. Agric. 19(2) : 13-19, 2008 ISSN 1017-8139 In vitro REGENERATION OF MUNGBEAN (Vigna radiata L.) FROM DIFFERENT EXPLANTS M. K. Khatun, M. S. Haque, S. Islam and K. M. Nasiruddin Department of Biotechnology,
More informationEffect of Genotype, Explant Type and Culture Medium on Shoot Regeneration in Tomato (Lycopersicon esculentum Mill.) in vitro
435 Bulgarian Journal of Agricultural Science, 12 (2006), 435-439 National Centre for Agrarian Sciences Effect of Genotype, Explant Type and Culture Medium on Shoot Regeneration in Tomato (Lycopersicon
More informationAn efficient protocol for clonal micropropagation of Mentha piperita L. (Pipperment)
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (4):518523 ISSN : 22497412 CODEN (USA): AJPSKY An efficient protocol for clonal micropropagation
More informationReceived : Accepted:
Ancient Science of Life Vol : XXVI (1) July, August, September 2004 Conservation of an endemic medicinal plant, Berberis tinctoria Lesch. In Nilgiris through micro propagation S.Paulsamy, S. Padmavathi
More informationDevelopment of a suitable plant regeneration protocol of cotton cultivar variety in comparison to non cultivar Coker
IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) ISSN: 2455-264X, Volume 2, Issue 5 (Jul. Aug. 2016), PP 14-20 Development of a suitable plant regeneration protocol of cotton cultivar variety
More informationIn vitro Plant Regeneration of Withania somnifera. Ujjwala Supe, Fanisha Dhote and M.G. Roymon
Plant Tissue Cult. & Biotech. 16(2): 111-115, 2006 (December) - Short communication PTC&B In vitro Plant Regeneration of Withania somnifera Ujjwala Supe, Fanisha Dhote and M.G. Roymon Plant Tissue Culture
More informationAVOCADO CALLUS AND BUD CULTURE
Proc. Fla. State Hort. Soc. 96:181-182. 1983. AVOCADO CALLUS AND BUD CULTURE M. J. Young University of Florida, IF AS, Fruit Crops Department, Gainesville, FL 32611 Additional index words, tissue culture,
More informationCONSIDERATIONS REGARDING THE EFFECTS OF GROWTH REGULATORS OVER THE IN VITRO MORPHOGENETIC REACTION AT ORIGANUM VULGARE L.
CRISTEA TINA OANA, FLTICEANU MARCELA, PRISECARU MARIA J. Plant Develop. 15 (2008): 133 138 CONSIDERATIONS REGARDING THE EFFECTS OF GROWTH REGULATORS OVER THE IN VITRO MORPHOGENETIC REACTION AT ORIGANUM
More informationIN VITRO PLANT REGENERATION STUDIES IN BRINJAL
Journal of Cell and Tissue Research Vol. 12(2) 3213-3218 (2012) ISSN: 0974-0910 (Available online at www.tcrjournals.com) Original Article IN VITRO PLANT REGENERATION STUDIES IN BRINJAL BARDHAN, S. K.,
More informationIN VITRO BUD CULTURE OF KINNOW TREE
Pak. J. Bot., 38(3): 597-601, 2006. IN VITRO BUD CULTURE OF KINNOW TREE Nuclear Institute for Agriculture Biology (NIAB), Faisalabad. Abstract Tissues from field grown trees have contamination problems
More informationSOMATIC EMBRYOGENESIS IN STRAWBERRY (FRAGARIA ANANASSA) VAR. CAMAROSA
G.J.B.B., VOL.6 (2) 17: 39-313 ISSN 2278 913 SOMATIC EMBRYOGENESIS IN STRAWBERRY (FRAGARIA ANANASSA) VAR. CAMAROSA Swapnil Gorakh Waghmare*, Komal Ramchandra Pawar, Ramling Haribhau Tabe, Abhijeet Shivajirao
More informationFactors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.) Maryam Vahedi*, Siamak Kalantari and Seyed Alireza Salami
Plant Tissue Cult. & Biotech. 24(1): 1 9, 2014 (June) PTC&B Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.) Maryam Vahedi*, Siamak Kalantari and Seyed Alireza Salami
More informationREGENERATION SYSTEMS FOR PYRAMIDING DISEASE RESISTANCE INTO WALNUT ROOTSTOCKS
REGENERATION SYSTEMS FOR PYRAMIDING DISEASE RESISTANCE INTO WALNUT ROOTSTOCKS John E. Preece, Ana María Ibáñez, Quyen Tran, Dio Gunawan, Chuck Leslie, David Tricoli, and Abhaya Dandekar ABSTRACT The purpose
More informationAdventitious Shoot Regeneration from Immature Embryo Explant Obtained from Female Female Momordica dioica
Plant Tissue Cult. & Biotech. 17(1): 29-36, 2007 (June) PTC&B Adventitious Shoot Regeneration from Immature Embryo Explant Obtained from Female Female Momordica dioica A. Hoque, M. Hossain, S. Alam, S.
More informationUse of Tissue culture to alleviate the supply of quality planting materials among cacao smallholder farmers in Cote d Ivoire
Use of Tissue culture to alleviate the supply of quality planting materials among cacao smallholder farmers in Cote d Ivoire Jane Kahia, Siaka Kone, Georges Ngoran, Lucien Diby.,Jean Claude Nzi and Christophe
More informationMicropropagation of Chlorophytum borivilliens through direct organogenesis
Available online at www.pelagiaresearchlibrary.com Advances in Applied Science Research, 2010, 1 (2): 41-46 ISSN: 0976-8610 CODEN (USA): AASRFC Micropropagation of Chlorophytum borivilliens through direct
More informationIn Vitro Shoot Regeneration and Development of Microcorms of Moroccan Saffron (Crocus sativus L.)
Atlas Journal of Plant Biology 2016 doi: 10.5147/ajpb.2016.0156 In Vitro Shoot Regeneration and Development of Microcorms of Moroccan Saffron (Crocus sativus L.) Khalid Lagram, Mohamed Ben El Caid, Souad
More informationGenetic and non genetic factors affecting callus induction and regeneration in sugarcane Sobhakumari V.P 1, Sreedivya M.J. 2 and Sanu Mary Abraham 3
Gregor Mendel Foundation Journal : -; 00. Genetic and non genetic factors affecting callus induction and regeneration in sugarcane Sobhakumari V.P, Sreedivya M.J. and Sanu Mary Abraham Tissue Culture Laboratory,
More informationapplication in propagating Butterfly attracting Plants
Plant Tissue Culture and its application in propagating Butterfly attracting Plants Kodiswaran Kandasamy Tissue Culture Unit Forest Biotechnology Division FRIM kodiswaran@frim.gov.my Cratoxylon formosum
More informationMicropropagation Scheme of Curcuma alismatifolia Gagnep.
Micropropagation Scheme of Curcuma alismatifolia Gagnep. N. Toppoonyanont, S. Chongsang, S. Chujan, S. Somsueb and P. Nuamjaroen Department of Biology, Faculty of Science, Maejo University Chiang Mai 50290
More informationMICROPROPAGATION OF CHRYSANTHEMUM (CHRYSANTHEMUM MORIFOLIUM) USING SHOOT TIP AS EXPLANT
MICROPROPAGATION OF CHRYSANTHEMUM (CHRYSANTHEMUM MORIFOLIUM) USING SHOOT TIP AS EXPLANT R. Nalini Department of Biotechnology, Adhiparasakthi Agricultural College (Affiliated to TNAU) G. B. Nagar, Kalavai,
More informationINTERNATIONAL JOURNAL OF RESEARCH AND REVIEWS IN PHARMACY AND APPLIED SCIENCES. Received: Accepted: Published:
ISSN:2249-1236 INTERNATIONAL JOURNAL OF RESEARCH AND REVIEWS IN PHARMACY AND APPLIED SCIENCES Research Article Received: 06-08-2011 Accepted: 12-09-2011 Published: 29-09-2011 OPTIMIZATION OF REGENERATION
More informationEFFECTIVE CALLUS INDUCTION AND PLANT REGENERATION IN BRASSICA NAPUS (L.) VAR DGS-1
Journal of Cell and Tissue Research Vol. 12(2) 3229-3234 (2012) ISSN: 0974-0910 (Available online at www.tcrjournals.com) Original Article EFFECTIVE CALLUS INDUCTION AND PLANT REGENERATION IN BRASSICA
More informationEffects of Different Concentrations of α-naphthaleneacetic Acid and 6-benzylaminopurine on Shoot Regeneration of Vinca minor L.
J. Agric. Sci. Technol. (2008) Vol. 10: 337-344 Effects of Different Concentrations of α-naphthaleneacetic Acid and 6-benzylaminopurine on Shoot Regeneration of Vinca minor L. F. Raouf Fard 1, A. Moieni
More informationLow-Cost Alternatives for Conventional Tissue Culture Media
International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 7 Number 04 (2018) Journal homepage: http://www.ijcmas.com Original Research Article https://doi.org/10.20546/ijcmas.2018.704.288
More informationEfficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk
Indian Journal of Biotechnology Vol 3, January 2004, pp. 113-118 Efficient micropropagation of Vanilla planifolia Andr. under influence of thidiazuron, zeatin and coconut milk P Giridhar and G A Ravishankar*
More informationFull length Article Studies on callus induction and shoot regeneration in Tomato
Science Research Reporter, 4(1): 89-93, (April - 2014) RUT Printer and Publisher (http://jsrr.net) ISSN: 2249-2321 (Print); ISSN: 2249-7846 (Online) Received: 15-01-2014, Revised: 15-02-2014, Accepted:
More informationTitle: Development of Micropropagation and Acclimation Protocols for the Commercialization of a New Bonsai Ornamaental Tree for the California Market.
Title: Development of Micropropagation and Acclimation Protocols for the Commercialization of a New Bonsai Ornamaental Tree for the California Market. Authors: Dan E. Parfitt 1, Helen M. Chan 2, and Ali
More information[BIO29] In vitro regeneration system of teak (Tectona grandis L.)
[BIO29] In vitro regeneration system of teak (Tectona grandis L.) Lim Sooi Ping 1, Maheran Abdul Aziz 1, Uma Rani Sinniah 2, Fadhilah Zainudin 3 1 Department of Agriculture Technology, Faculty of Agriculture,
More informationHigh Frequency Shoots Regeneration from Cotyledon Explants of Teasle Gourd Via Organogenesis
High Frequency Shoots Regeneration from Cotyledon Explants of Teasle Gourd Via Organogenesis Mohammad Abdul Karim Dept. of Crop Botany, Faculty of Agriculture, Bangladesh Agricultural University, Mymensingh2202,
More informationComparison of Regeneration Efficiency of Three Different Genotypes of Basmati Rice under in Vitro Condition
Comparison of Regeneration Efficiency of Three Different Genotypes of Basmati Rice under in Vitro Condition Ritu Mahajan* and Shilpi Sharma School of Biotechnology, University of Jammu, Jammu, India Abstract
More informationIN VITRO CALLUS INITIATION AND ORGANOGENESIS FROM SHOOT TIP EXPLANTS OF TINOSPORA CORDIFOLIA (WILLD.) MIERS EX HOOK. F & THOMS
IN VITRO CALLUS INITIATION AND ORGANOGENESIS FROM SHOOT TIP EXPLANTS OF TINOSPORA CORDIFOLIA (WILLD.) MIERS EX HOOK. F & THOMS Hemant Sharma 1 and Vashistha B.D. 2 1 Department of Botany, Dayanand P.G.
More informationRapid in vitro regeneration of Gerbera jamesonii (H. Bolus ex Hook. f.) from different explants
Indian Journal of Biotechnology Vol 3, October 2004, pp 584-588 Rapid in vitro regeneration of Gerbera jamesonii (H. Bolus ex Hook. f.) from different explants Purnima Tyagi and S L Kothari* Botany Department,
More informationIn Vitro Formation of Gerbera (Gerbera hybrida Hort.) Plantlets through Excised Scape Cultures
J. Japan. Soc. Hort. Sci. 52(1) : 45-50. 1983. In Vitro Formation of Gerbera (Gerbera hybrida Hort.) Plantlets through Excised Scape Cultures Chien-young CHU and Min-chang HUANG Department of Horticulture,
More informationRegeneration potential of seedling explants of chilli (Capsicum annuum)
African Journal of Biotechnology Vol. 8 (4), pp. 591-596, 18 February, 2009 Available online at http://www.academicjournals.org/ajb ISSN 1684 5315 2009 Academic Journals Full Length Research Paper Regeneration
More informationIN VITRO PROPAGATION OF THE BALKAN ENDEMIC SPECIES VERBASCUM ERIOPHORUM GODR. Abstract. Introduction
767 Bulgarian Journal of Agricultural Science, 22 (No 5) 2016, 767 771 Agricultural Academy IN VITRO PROPAGATION OF THE BALKAN ENDEMIC SPECIES VERBASCUM ERIOPHORUM GODR. Zh. P. YORDANOVA *, M. A. ROGOVA
More informationPLANT REGENERATION FROM PROTOCORM-DERIVED CALLUS OF CYPRIPEDIUM FORMOSANUM
In Vitro Cell. Dev. Biol. Plant 39:475 479, September October 2003 q 2003 Society for In Vitro Biology 1054-5476/03 $18.00+0.00 DOI: 10.1079/IVP2003450 PLANT REGENERATION FROM PROTOCORM-DERIVED CALLUS
More informationSTUDIES ON DATE PALM IN TISSUE CULTURE
STUDIES ON DATE PALM IN TISSUE CULTURE By GEHAN MOHAMED YOUSRY SALAMA B.Sc. Agric. Sci. (Horticulture), Fac. Agric., Ain Shams Univ., 1990 M.Sc. Agric. Sci. (Ornamental Horticulture), Fac. Agric., Cairo
More informationResearcher, 1(3), 2009, Micropropagation Of Prosopis Cineraria (L.) Druce A Multipurpose Desert Tree
Micropropagation Of Prosopis Cineraria (L.) Druce A Multipurpose Desert Tree Surender Kumar And Narender Singh Department Of Botany, Kurukshetra University Kurukshetra (India) E.mail: suren_kr@yahoo.co.in
More informationMicropropagation of Sterile and Non-Flowering Nicotiana Lines
2008 The Japan Mendel Society Cytologia 73(1): 9 13, 2008 Micropropagation of Sterile and Non-Flowering Nicotiana Lines Sarala K.*, Rao R. V. S., Murthy T. G. K. and Satyavani J. V. R. Central Tobacco
More informationInternational Journal of Current Biotechnology
Prasad M. P., Invitro optimization of growth hormones in the micropropagation of Gerbera species, Int.J.Curr.Biotechnol., 2014, 2(2):15. International Journal of Current Biotechnology ISSN: 2321 8371 Journal
More informationSUSTAINABLE AND INTEGRAL EXPLOITATION OF AGAVE
SUSTAINABLE AND INTEGRAL EXPLOITATION OF AGAVE Editor Antonia Gutiérrez-Mora Compilers Benjamín Rodríguez-Garay Silvia Maribel Contreras-Ramos Manuel Reinhart Kirchmayr Marisela González-Ávila Index 1.
More informationIN VITRO BULBLET INDUCTION FROM BULB SCALES OF ENDANGERED ORNAMENTAL PLANT MUSCARI AZUREUM
Article DOI: 10.2478/v10133-010-0024-4 A&EB IN VITRO BULBLET INDUCTION FROM BULB SCALES OF ENDANGERED ORNAMENTAL PLANT MUSCARI AZUREUM S. Uranbey 1, A. Ipek 1, M. Caliskan 2, E. Dundar 3, S. Cocu 4, D.
More informationA rapid in vitro propagation protocol of Bunium persicum (Boiss.)B.Fedtsch. growing in Kashmir, Himalaya
Species RESEARCH International Journal for Species ISSN 2319 5746 EISSN 2319 5754 A rapid in vitro propagation protocol of Bunium persicum (Boiss.)B.Fedtsch. growing in Kashmir, Himalaya Iram Bashir 1,
More informationEFFECT OF GROWTH HORMONES ON SHOOT PROLIFERATION OF ROSE CULTIVARS
Pak. J. Bot., 37(4): 875-881, 2005. EFFECT OF GROWTH HORMONES ON SHOOT PROLIFERATION OF ROSE CULTIVARS M. JAFAR JASKANI 1, M. QASIM 1, JAVERIA SHERANI 2, ZAHOOR HUSSAIN 1 AND HAIDER ABBAS 3 1 Institute
More informationGRADUATE UNIVERSITY OF SCIENCE AND TECHNOLGY VU THI HIEN
MINISTRY OF EDUCATION AND TRAINING VIETNAM ACADEMY OF SCIENCE AND TECHNOLOGY GRADUATE UNIVERSITY OF SCIENCE AND TECHNOLGY ----------------------------- VU THI HIEN REGENERATION AND MICROPROPAGATION OF
More informationChapter 2 Indirect Organogenesis and histological analysis of organogenic and non-organogenic calli obtained from in vitro
Chapter 2 Indirect Organogenesis and histological analysis of organogenic and non-organogenic calli obtained from in vitro cultures of Justicia adhatoda L. 2.1. ABSTRACT Leaf, axillary bud and root tip
More informationEFFECT OF GROWTH REGULATORS ON MERISTEM TIP CULTURE OF LOCAL POTATO CVS DESIREE AND PATRONES
ISSN 1023-1072 Pak. J. Agri., Agril. Engg., Vet. Sci., 2011, 27 (2): 143-149 5 EFFECT OF GROWTH REGULATORS ON MERISTEM TIP CULTURE OF LOCAL POTATO CVS DESIREE AND PATRONES A. Yasmin 1, A. A. Jalbani 2
More informationDirect Regeneration of Shoot from Axillary Bud of Citrus Reticulate
Available online http://www.ijat-aatsea.com ISSN 1686-9141 Direct Regeneration of Shoot from Axillary Bud of Citrus Reticulate Shende, C. B. and Manik, S. R. Department of Botany Mohsinbhai Zaweri Arts,
More informationRapid micropropagation and callus induction of Terminalia bellerica Roxb. - An endangered plant
Available online at www.pelagiaresearchlibrary.com Asian Journal of Plant Science and Research, 2012, 2 (3): 364368 ISSN : 22497412 CODEN (USA): AJPSKY Rapid micropropagation and callus induction of Terminalia
More informationESTABLISHMENT OF A SUITABLE AND REPRODUCIBLE PROTOCOL FOR IN VITRO REGENERATION OF GINGER (ZINGIBER OFFICINALE ROSCOE)
Pak. J. Bot., 42(2): 1065-1074, 2010. ESTABLISHMENT OF A SUITABLE AND REPRODUCIBLE PROTOCOL FOR IN VITRO REGENERATION OF GINGER (ZINGIBER OFFICINALE ROSCOE) ANWAR HOSSAIN 1, LUTFUL HASSAN 1 *, ABDUL KHALEQ
More informationThe application of leafy explant micropropagation protocol in enhancing the multiplication ef ciency of Alstroemeria
Scientia Horticulturae 85 (2000) 307±318 The application of leafy explant micropropagation protocol in enhancing the multiplication ef ciency of Alstroemeria Hsueh-Shih Lin 1, Marjo J. De Jeu *, Evert
More informationAdventitious Shoot Formation on Teak (Tectona grandis L.f.) Callus Cultures Derived from Internodal Segments
Adventitious Shoot Formation on Teak (Tectona grandis L.f.) Callus Cultures Derived from Internodal Segments Sri Nanan Widiyanto, Dwi Erytrina and Heni Rahmania Department of Biology, Institut Teknologi
More informationPlant regeneration of Anthurium andreanum cv Rubrun
Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.7 No.3, Issue of December 15, 2004 2004 by Pontificia Universidad Católica de Valparaíso -- Chile Received June 4, 2004 / Accepted November 9, 2004
More informationIn vitro propagation and whole plant regeneration from callus in Datura (Datura stramonium. L)
African Journal of Biotechnology Vol. 10(3), pp. 442-448, 17 January, 2011 Available online at http://www.academicjournals.org/ajb DOI: 10.5897/AJB09.1968 ISSN 1684 5315 2011 Academic Journals Full Length
More informationAmerican-Eurasian Journal of Sustainable Agriculture, 6(4): , 2012 ISSN Somatic Embryogenesis and Plantlet Regeneration in Amla
American-Eurasian Journal of Sustainable Agriculture, 6(4): 417-421, 212 ISSN 1995-748 417 ORIGINAL ARTICLE Somatic Embryogenesis and Plantlet Regeneration in Amla L. Al-Sabah, C. Sudhersan and S. Jibi
More informationThe effect of plant growth regulators on optimization of tissue culture system in Malaysian upland rice
African Journal of Biotechnology Vol. 9(14), pp. 2089-2094, 5 April, 2010 Available online at http://www.academicjournals.org/ajb ISSN 1684 5315 2010 Academic Journals Full Length Research Paper The effect
More informationOrganogenic plant regeneration via callus induction in chickpea (Cicer arietinum L.) Role of genotypes, growth regulators and explants
Indian Journal of Biotechnology Vol. 4, April 2005, pp. 251-256 Organogenic plant regeneration via callus induction in chickpea (Cicer arietinum L.) Role of genotypes, growth regulators and explants Anju
More informationSweta Mishra and Yuvraj K Vala
2018; 7(2): 91-96 E-ISSN: 2278-4136 P-ISSN: 2349-8234 JPP 2018; 7(2): 91-96 Received: 07-01-2018 Accepted: 08-02-2018 Sweta Mishra Biotechnology Section, Central Instrumentation Laboratory, Sardarkrushinagar
More informationIn Vitro Flowering and Shoot Multiplication from Nodal Explants of Ceropegia bulbosa Roxb. var. bulbosa
Taiwania, 48(2): 106-111 In Vitro Flowering and Shoot Multiplication from Nodal Explants of Ceropegia bulbosa Roxb. var. bulbosa S. John Britto (1, 2), E. Natarajan (1) and D. I. Arockiasamy (1) (Manuscript
More informationIN VITRO INDUCTION OF HAPLOID IN EGGPLANT (SOLANUM MELONGENA L.)
146 Capsicum and Eggplant Newsletter 22 (2003): 147-150. IN VITRO INDUCTION OF HAPLOID IN EGGPLANT (SOLANUM MELONGENA L.) Sanjeev Kumar, Major Singh, Prabhavathi K. and Amit Mathews Indian Institute of
More information