Sterisonic GxP Series Cell Culture Incubators

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1 Sterisonic GxP Series Cell Culture Incubators CO 2 Incubators MCO-19AIC(UVH) MCO-19AIC(UV) MCO-19AIC CO 2 /O 2 Incubators MCO-19M(UVH) MCO-19M(UV) MCO-19M Features: The industry s most complete cell culture solution for highly regulated applications or conventional incubation. Safe, effective and documented two-hour in situ H 2 O 2 sterilization for fastest decontamination. My Life. My Work. My Choice.

2 MCO-19AIC MCO-19AIC(UV) MCO-19AIC(UVH) MCO-19M MCO-19M(UV) MCO-19M(UVH) Sterisonic GxP Series Cell Culture CO 2 and CO 2 / O 2 Incubators The industry s most complete cell culture solution for highly regulated applications or conventional incubation. Now with safe, effective and documented two-hour in situ H 2 O 2 sterilization for fastest decontamination turn-around and maximum availability. Sterisonic GxP Selection CO 2 Incubators CO 2/O 2 Incubators Feature MCO- 19AIC(UVH) MCO- 19AIC(UV) MCO- 19AIC MCO- 19M(UVH) MCO- 19M(UV) H2O2 Vapor Sterilization Standard Optional Optional Standard Optional Optional SafeCell UV Decontamination Standard Standard Optional Standard Standard Optional Single Beam, Dual Detector IR CO2 Sensor Standard Standard Standard Standard Standard Standard Oxygen Concentration Control, Zirconia Control N/A N/A N/A Standard Standard Standard MCO- 19M Product Applications The combination of Sterisonic GxP incubator performance functions permit use with confidence in high-value cell protocols among hard-to-grow cell lines, cells highly sensitive to contamination, ultra-sensitive media and reagents, or protocols that require a strict isolation and sterilization between processes. These include but are not limited to: Stem cell research Autologous tissue regeneration and regenerative medicine In vitro fertilization 1 Genomic and proteomic expression Esoteric plant and amphibian cell culture Hypersensitive and transgenic cell culture Low media volume microplate work MCO-19AIC(UVH) Designed for use with a variety of standard cell culture vessels and protocols. Interior components and adjustable shelves are configured for easy access, in situ sterilization and flexible arrangement for a variety of applications. Four adjustable shelves are included, standard; maximum shelf capacity is 15 shelves. MCO-19M Multi-Gas Incubator, Oxygen and CO 2 Control Ideal for in vitro fertilization (IVF), genetic research, regenerative medicine and other protocols that require CO2 and sub-ambient (hypoxic) or above-ambient oxygen control. Cabinet based on MCO-19 platform with incu safe copper-enriched stainless steel chamber, continuous contamination control, patented D.H.A. multi-point, air-jacketed temperature control system, elevated relative humidity with integral water-level sensor (k) clearance applied for. Contact SANYO for status. MCO-19AIC and MCO-20AIC CO 2 incubators have received U.S. Food and Drug Administration 510(k) clearance for in vitro fertilization applications in accordance with the FDA Safe Medical Devices Act of 1990 and the Medical Device Amendments of Reference: Number K Regulation Number: 21 CFR , Assisted Reproduction Accessories, Regulatory Class II, Product Code 85MOG, October 30, Sterisonic GxP Series Cell Culture Incubators

3 Sterisonc GxP Features and Benefits The SANYO Sterisonic GxP is designed for a wide array of demanding and highly regulated applications in the biomedical, pharmaceutical, medical research and clinical laboratory. Representing years of research, development and component testing, the Sterisonic GxP incorporates a collective of mutually functional systems and design attributes to offer a holistic solution to cell culture protocols, from the most sophisticated to more familiar and conventional processes. Sterilization and Decontamination The unique Sterisonic GxP H 2O 2 sterilization system limits downtime to less than three hours when total chamber sterilization with verification is desired. All interior components and CO2 sampling loop are sterilized in situ; no need for removal and autoclaving. Active Background Contamination Control fights contamination while cell culture protocols are in process. The patented SafeCell UV system scrubs interior airflow to destroy airborne and humidity pan contaminants. Exclusive incu safe copperenriched stainless steel interior surfaces assure constant germicidal protection. Shelf brackets are formed with an exaggerated angle to minimize surface contact with flat shelves*. Control and Monitoring The Sterisonic GxP control and information center includes an intuitive pop-up menu, high resolution LCD for inputs, outputs and performance at-a-glance. Multi-point data logging offers push-button graphical display. An optional PC interface permits remote transmission for GMP/GLP protocols as required. Precise PID logic controls and adjusts to all temperature, CO 2 setpoints and alarm parameters. CO 2 Control SANYO proprietary single-beam, dual detector infrared (IR2) CO 2 sensor delivers precise CO 2 control, quick recovery following door openings, and auto sampling with no moving parts. Continuous zero calibration is standard. An optional semi-automatic, one-point calibration system is available. Catalog No. MCO-SG; see Accessories. Zirconia O 2 Control System, MCO-19M Series A zirconia oxygen sensor maintains sub-ambient O 2 levels from 1% to 18%. Additionally, enriched O 2 levels from 22% to 80% are enabled with proper safety precautions. Concurrently the MCO-19M permits a CO 2 range 0% to 20% via infrared sensor. Nitrogen gas bubbler accelerates recovery of chamber humidity levels following door openings. An electronic P.I.D. control maintains accurate temperature and gas setpoints over the entire system range. The MCO-19M includes an automatic gas switchover system that changes from the primary to a secondary gas cylinder for either oxygen or nitrogen; an optional second gas switchover system is available for CO 2. Cabinet based on MCO-19 platform with incu safe copper-enriched stainless steel chamber, continuous contamination control, patented D.H.A. multi-point, air-jacketed temperature control system, elevated relative humidity with integral water-level sensor. MCO-19M MCO-19M shown with four separate inner doors with gaskets. 3

4 Sterisonc GxP Features and Benefits Temperature and Humidity Control The patented Direct Heat and Air conditioning system manages setpoint temperature through multiple, variable warming points under microprocessor control. The humidity pan is easy to fill, easy to clean; the automatic optical sensor advises of low water level. H 2 O 2 Sterilization The use of H 2O 2 sterilization in biological safety cabinets and barrier isolators is a popular alternative to ethylene oxide (EtO) as a safer, more efficient decontamination method. H 2O 2 has long been widely used in the pharmaceutical industry. In aerospace research, H 2O 2 is used to sterilize satellites. The FDA has recently granted 510(k) clearance to use H 2O 2 in individual medical device manufacturing applications. EtO criteria outlined in ANSI/AAMI/ISO may be used as a validation guideline. For references online visit Unlike conventional incubators, unique features of the SANYO Sterisonic GxP incubator permit use of the H 2O 2 process in situ with complete safety, zero impact on adjacent equipment or the environment, and speed to return the incubator to service. The H 2O 2 sterilization process functions with the patented SANYO SafeCell UV system. Following a seven-minute H 2O 2 vaporization, circulation and dwell cycle, vaporization is stopped and the SafeCell UV lamp turned ON for up to ninety minutes. When exposed to UV light, the H 2O 2 vapor breaks down into water and oxygen, leaving only traces of water droplets. These droplets automatically condense onto a naturally cooler section of the interior floor for easy wipe-up. Throughout the entire cycle the Sterisonic GxP airflow system continues to gently circulate interior air assuring 100% vapor contact with all interior surfaces, ultimately creating a serial dilution of H 2O 2 as it passes over the UV lamp. Orientation of interior sample ports of the single beam, dual detector IR CO 2 sensor creates a slight Venturi flow through the sample chamber, permitting total sterilization of the CO 2 system at the same time. Shape and location of interior components such as shelves, shelf brackets, plenum covers and the humidity pan permit the components to remain in the chamber during the sterilization process, conveniently bypassing the need for a separate autoclave cycle. Once the cycle is complete, the door locking system is released; the inner door can be opened, interior components repositioned and the incubator is returned to service. Ergonomic Cabinet Design With reversible inner and outer doors, a single SANYO incubator offers the industry's most flexible installation options available. Low profile cabinet with door-mounted control panel permits easy access and viewing. The outer door latch and door heater cable is easily switched if a reverse opening is required. Sterisonic GxP Series H 2 O 2 Sterilization Performance Value 2 Hours 14 Hours SANYO Sterisonic Brand X H2O2 sterilization vs. high heat sterilization = Uptime (Hours) = Downtime (Hours) The documented two-hour in situ H 2O 2 sequence puts the fully sterilized Sterisonic GxP available and ready for use quicker than any other incubator worldwide. 4 Sterisonic GxP Series Cell Culture Incubators

5 Sterisonc GxP Features and Benefits Cabinet knock-outs are pre-drilled and tapped to eliminate drilling and to simplify re-mounting of door hardware. The outer door closes against a soft, easily cleaned magnetic gasket designed to eliminate ambient air shear across the glass inner door, minimizing condensation. A door ajar alarm provides an audible and visual warning if the outer door is left open. A center-mounted key lock is located beneath the door for added security; model MCO-19AIC(UVH) only. Shelves and Inventory Management Convenient space efficient inventory management is simplified through a system of adjustable, extendable shelves. Inventory shelves and brackets are formed from polished copper-enriched stainless steel, removable without tools, and can remain inside the incubator during the H 2O 2 sterilization cycle or autoclaved separately if desired. Shelves are perforated to permit natural vertical air convection through and around labware. Shelf brackets are formed with an exaggerated angle to minimize surface contact with flat shelves (patent pending). Brackets slip easily into vertical supports that attach to interior chamber walls with clearance sufficient to permit proper air circulation. Inner Door and Gasket The inner design is critical to successful contamination control technique. The inner gasket body forms an effective thermal transition between the ambient air and warm, humidified incubator atmosphere, minimizing condensation and eliminating moisture traps which can harbor contaminants. The inner door gasket is a dual durometer extrusion (two levels of softness) from closed-cell silicone to inhibit contamination. The gasket feather-edge allows the inner glass door to close gently against the chamber opening for a tight peripheral seal. The entire inner door gasket is removable for cleaning and/or replacement if required. Radiant heat from the outer door, apportioned by the microprocessor MCO-38AIC(UVH) Stacking Doubles Capacity in Same Footprint. The Sterisonic GxP cabinet is designed for stacking, allowing one unit to be positioned on top of another, doubling interior volume without additional floor space. The combination of stacking and reversible doors offers the most installation options possible. An optional roller base is recommended for stacked installations to permit mobility if required; see Accessories. control as a function of the patented Direct Heat and Air Jacket, automatically warms the inner door glass in proportion to total heat demand and condensation control. Field Reversible Door The field-reversible door allows universal installation using the left-hand hinge (standard) or a right-hand hinge modification The outer door includes a universal finger grip at each side. Mounting holes for hinge hardware are pre-drilled and capped with easily removable trim plugs. The door heater cable plugs into the alternate connection to complete the change. Elevated Humidity, Low Water Level Warning To avoid cell culture desiccation, the SANYO Sterisonic GxP CO 2 incubator maintains ~95% RH at 37 C. Humidification is achieved by a combined forced-air and natural evaporation method enhanced by the Direct Heat and Air Jacket base heater, and protected by an optical water level indicator to warn of low water in the removable humidity pan. The humidity pan removes easily for regular cleaning and refill. When filled, the pan slides into place. The optical sensor returns to position and the SafeCell UV lamp destroys any contaminants introduced during the process. For the H2O 2 in situ sterilization cycle the humidity pan is repositioned against the chamber wall to eliminate the need for separate autoclaving. 5

6 Sterisonic GxP Control System Intelligent Interface Through Integrated LCD Control with Graphical Display The Sterisonic GxP incubator is managed by an integrated microprocessor controller with LCD graphical display to simplify all incubator functions. Stable temperature and humidity conditions are achieved through a combination of performance systems supervised by the controller complete with alarm, programming, calibration and diagnostic protocols. Control Features The Sterisonic GxP control and information center includes an intuitive pop-up menu, high resolution LCD for inputs, outputs and performance at-a-glance. Multi-point data logging offers pushbutton graphical display. An optional PC interface permits remote transmission for GMP/GLP protocols as required. Selected performance histories can be acquired in graphical format for easy interpretation. Precise PID logic controls and adjusts to all temperature and CO 2 setpoints and alarm parameters. A range of setpoint, alarm and programmable inputs are established through pop-up menus and function keys. Standard parameters are factory-set for quick start-up, and all parameters can be changed as required. Logged parameters can be exported to remote databases, off-site alarm or data capture systems through optional communications board for compliance monitoring; see Accessories. A remote alarm terminal mounted at the rear of the cabinet can be connected to an external alarm system. Tactile feedback, touch pad data shift and entry keys simplify operation. The control panel is center mounted in the outer door for easy access and viewing. Sterilization Cycle The H 2O 2 sterilization cycle is monitored for safety and cycle status. A physical interlock and neutralization sequence assures total sterilization and operator safety. 1 Start Cycle: When the H 2O 2 button is pressed a confirming message prompts the user to proceed with the sterilization cycle or cancel. 2 H 2O 2 Vapor Cycle: Once the door locks automatically, the cycle starts. The flashing H 2O 2 display confirms the process and counts down remaining H 2O 2 vaporization time. 3 UV Resolution: The H 2O 2 atomizer automatically completes after a 7 minute cycle. UV lamp comes ON. The flashing UV Resolve display counts down remaining time in the UV cycle as H 2O 2 is reduced to water and trace oxygen. 4 Cycle Complete: When the cycle is complete the door lock releases automatically. The H 2O 2 atomizer and cable can be disconnected and removed and all interior components restored to their normal position. Sterilize: 7 min. SANYO H2O2 atomizer creates vapor which is circulated throughout chamber by interior blower. PREP: 15 min. Reposition shelves, humidity pan and plenum inside SANYO chamber. Interior surfaces are exposed. Start Cycle: 30 min. Press H2O2 start button. Chamber warms to 45 C. Resolve: 90 min. UV lamp glows for 90 minutes, reducing H2O2 to harmless water droplets. Finish. Shelves, humidity pan and plenum are returned to operating position. Sterisonic GxP H 20 2 Sterilization Cycle (3 Hours, total) Elapsed Time: 15 min. 30 min. 45 min Conventional High Heat Decontamination (24 Hours, total) PREP: 15 min. Remove interior components sensitive to high heat. Start Cycle: 90 min. Interior chamber elevates to high heat. Sterilize: 14 hours Interior chamber remains at high heat. 6 Sterisonic GxP Series Cell Culture Incubators

7 Sterisonic GxP Control System Digital alphanumeric LCD display. Message display Pop-up menu 1 Visual alarm indicator H 2O 2 sterilization sequence start key 2 Menu call button Display contrast adjustment 3 Positive feedback tactile input buttons Positive feedback tactile entry and function keys Finish. Incubator must cool from high heat temperatures to near ambient. 7

8 Sterisonic GxP Design and Technology All SANYO CO 2 incubators feature patented Direct Heat and Air Jacket temperature control for accurate, uniform temperature control and incu safe for continuous contamination control. SANYO laboratory CO 2 incubators feature selected SafeCell UV with exclusive, patented Active Background Contamination Control. Direct Heat and Air Jacket Heating System Zone Location Function Energy Microprocessor Controller Main (Red) Side, top and rear walls Dominant heat source Variable Base (Yellow) Floor Base heater elevates the humidity reservoir to achieve 95% RH at 37 C Variable Energizes any, all or in combination of heating elements as required. Front (Green) Outer door Warms the inner glass in response to ambient conditions; eliminates condensation on glass and around the opening and promotes temperature uniformity Variable Air Jacket (White) Side, top and rear walls Sealed, surrounds interior chamber with natural air convection and promotes chamber wall uniformity Insulation (Gray) Side, top and rear walls, door Promotes energy efficiency, mitigates effect of ambient temperature fluctuations on air jacket Direct Heat and Air Jacket Heating System The patented Direct Heat and Air Jacket surrounds the inner walls with a natural convection airflow that converts to radiant wall heat through thermal conduction to achieve accurate, uniform and highly responsive temperature control within the chamber. The microprocessor controller directs proportional distribution of power to independent heating sources surrounding the chamber. Arranged in three zones, these sources include the side, top and rear walls, the chamber base and the outer door. Each zone is controlled by the microprocessor which manages continuous feedback from the incubator chamber sensors. The patented Direct Heat and Air Jacket heating system distributes proportional energy to the interior chamber through a natural convection air jacket surrounded by a high density insulation to protect against ambient temperature fluctuations H 2 O 2 Sterilization The unique Sterisonic GxP H 2O 2 sterilization system limits downtime to less than three hours when total chamber sterilization is desired. All interior components and CO2 sampling loop are sterilized in situ; no need for removal and autoclaving. Available as standard on the MCO-19AIC(UVH) and MCO-19M(UVH); optional on the MCO-19AIC(UV) and MCO-19M(UV). Following sterilization, SANYO Active Background Contamination Control fights contamination while cell culture protocols are in process. SafeCell UV System Sterisonic GxP Models UVH and UV include SafeCell UV contamination control technology based on an integrated combination of narrow bandwidth, ozone-free ultraviolet light, ceramic infrared CO 2 control, incu safe copper-enriched stainless steel alloy interiors and Direct Heat and Air Jacket heating managed by a microprocessor controller. 8 Sterisonic GxP Series Cell Culture Incubators

9 Sterisonic GxP Design and Technology These incubators offer the industry's most stable cell culture environment and are useful for the most critical applications where continuous contamination control is essential to cell viability. SANYO SafeCell UV Series CO 2 incubators offer significant economic benefits by minimizing interruptions for decontamination, by improving cell culture growth and expression under stable, repeatable conditions, and by minimizing the potential for product loss. SafeCell UV includes a programmable ultraviolet lamp, isolated from cell cultures, that sterilizes conditioned air and humidity reservoir water to prevent contamination without affecting cell cultures in vitro. SafeCell UV inhibits the growth of mycoplasma, bacteria, molds, spores, yeasts and fungi without costly HEPA filter air scrubbers which accumulate contaminants in the chamber air. High temperature decontamination systems, which can actually encourage in vitro growth of heat resistant thermophilic and hyperthermophilic microorganisms, are avoided. incu safe interior surfaces provide natural resistance to contamination. The SafeCell UV lamp reduces H 2O 2 to trace sterile water condensate following the seven-minute vapor sterilization process. Dual Wavelength Infrared CO 2 Control System The SANYO single beam, dual detector infrared CO 2 system offers unprecedented control accuracy and stability by simultaneously measuring two wavelengths. The chamber sample and a reference is configured around a ceramic-based sensor linked to the microprocessor controller with a sophisticated P.I.D./R (proportional, integral and derivative) algorithm. Benefits include ultra-fast recovery without overshoot and accurate CO 2 averages during periods of frequent incubator access with multiple door openings. The ceramic-based technique is impervious to changes in temperature and relative humidity, highly stable during door openings, and sensitive to restoration of setpoint equilibrium when the door is closed. The sensor maintains a continuous auto-zero function for accuracy. Actual CO2 is displayed on the main control panel. A CO2 sample port mounted on the incubator front permits convenient confirmation of chamber CO 2 density. An optional automatic CO2 switchover system is available; see Accessories. A two-stage regulator from the supply cylinder to the incubator is required; see Accessories. MCO-19M Series Multi-Gas Performance O 2 Control System In addition to precise CO 2 control, the MCO-19M Series incubator delivers exceptional O 2 control in support of cell culture processes which require belowambient or above-ambient oxygen levels. These include in vitro fertilization (IVF), gene research, regenerative medicine and emerging applications that are sensitive to oxygen levels other than ambient. A zirconia oxygen sensor maintains sub-ambient O 2 levels from 1% to 18%. Additionally, enriched O 2 levels from 22% to 80% are enabled with proper safety precautions. Concurrently the MCO-19M permits a CO 2 range 0% to 20% via infrared sensor. Nitrogen gas bubbler accelerates recovery of chamber humidity levels following door openings. An electronic P.I.D. control maintains accurate temperature and gas setpoints over the entire system range. The MCO-19M includes an automatic gas switchover system that changes from the primary to a secondary gas cylinder for either oxygen or nitrogen; an optional second gas switchover system is available for CO 2. Cabinet based on MCO-19 platform with incu safe copper-enriched stainless steel chamber, continuous contamination control, patented D.H.A. multi-point, air-jacketed temperature control system, elevated relative humidity with integral water-level sensor. incu safe Construction for Germicidal Protection SANYO offers exclusive use of incu safe copper-enriched stainless steel alloy interior surfaces within a technical design created to eliminate contamination sources and to mitigate the effect of airborne contaminates introduced through normal use. Selected to provide natural germicidal protection without rust or corrosion, incu safe expresses a natural germicidal attribute to inhibit the growth of molds, fungi, mycoplasma and bacteria. All interior components, including the air management plenum, shelf supports, humidity pan and blower wheel assembly are easily removable without tools if required. During the H2O 2 sterilization cycle interior components can be repositioned within the chamber for in situ sterilization. When components are removed, all interior surfaces are exposed for conventional wipe down. Large curve corners and electropolished surfaces are easy to clean. Pass thru ports accommodate probes or instrumentation leads as required for specialized cell culture protocols. Each chamber includes a port positioned in the interior, rear wall, upper left, with dual, non-voc silicone stoppers inside and outside the cabinet for added protection. 9

10 Sterisonic GxP Design and Technology Active Background Contamination Control A continuous Active Background Contamination Control process eliminates contamination without downtime. At the base of the plenum an isolated beam of high intensity, ozone-free ultraviolet light destroys contaminants in the air and in the humidity water reservoir, away from active cell cultures. SafeCell UV Lamp Program Cycles Mode After H2O2 Vaporization After Door Opening OFF 24 Hour Continuous ON Function The UV lamp automatically cycles ON for up to 90 minutes following the seven-minute H2O2 vapor cycle, reducing the H2O2 to water droplets. These droplets automatically condense onto a naturally cooler section of the interior floor for easy wipe-up. UV lamp automatically ON for five minutes after door is closed decontaminates incoming room air. If UV protection is not desired. Useful for overnight decontamination prior to first use, clinical decontamination protocols between patients, or following total chamber wipe-out after maintenance or service. Contaminants trapped within the distilled water pan are destroyed by ultraviolet light. Sterile, humidified air is released from the lower plenum for vertical convection through and around the perforated shelves. Interior air motion is suspended when the door is opened, minimizing movement of room air contaminants into the chamber. UV light is isolated by the plenum cover to protect cell cultures Airborne contaminants are eliminated by an automatic UV cycle that automatically turns ON for a specified period after each door opening. Trace contaminants that attach to interior surfaces are destroyed by the passive germicidal properties of the incu safe surfaces. The SafeCell UV lamp cycle is factory set for normal use, and can be re-programmed as desired by entering parameters through the central microprocessor control panel. Program parameters for the H 2O 2 sterilization cycle are non-adjustable for operator safety. Mycoplasma Survival Results Mycoplasma Strain Mycoplasma fermentans PG18 Mycoplasma orale CH19299 Mycoplasma arginini G230 Mycoplasma hominis PG21 Positive Control Conventional Type 304 Stainless Steel SANYO InCu safe Conventional Copper C1100 survival no survival survival survival survival no survival survival survival survival no survival survival survival survival no survival survival survival Chart summarizes test results with four strains of mycoplasma. Results demonstrate how SANYO InCu safe copper enriched stainless steel alloy offers germicidal properties of conventional C1100 copper while maintaining both corrosion-proof and discoloration-resistant properties of conventional Type 304 stainless steel. Detailed test results are available from SANYO. 10 Sterisonic GxP Series Cell Culture Incubators

11 Sterisonic GxP Specifications Sterisonic GxP Series Energy, Electrical and Utilties Description MCO-19AIC MCO-19AIC(UV) MCO-19AIC(UVH) MCO-19M MCO-19M(UV) MCO-19M(UVH) Maximum Power Consumption 310W 310W Maximum Heat Discharge 1062 BTU 1062 BTU Electrical Connection 115V,60Hz, 1 phase, NEMA 5-15P plug provided; requires NEMA 5-15R grounded receptacle. 115V,60Hz, 1 phase, NEMA 5-15P plug provided; requires NEMA 5-15R grounded receptacle. CO2 Gas Connection 4 to 6 mm inner diameter tubing 4 to 6 mm inner diameter tubing CO2 Gas Input Pressure Nominal 4.3 psi from two-stage CO2 regulator Nominal 4.3 psi from two-stage CO2 regulator CO2 Gas Cylinder Switchover System Optional Optional O 2 Control System N/A Microprocessor PID, Zirconia Sensor O 2 Range and Variation N/A 1-18%, 22-80% N 2 /O 2 Inlet Connect/Pressure N/A Nominal 7 psi from two-stage regulator N 2 /O 2 Switchover System N/A Standard Sterisonic GxP Series Dimensions, Weights and Capacities Model Number Volume (cu.ft.) Exterior Dimensions (w x f-b x h) Interior Dimensions (w x f-b x h) Shelves Net Weight (nominal) MCO-19AIC MCO-19AIC(UV) MCO-19AIC(UVH) L 24.4" x 27.9" x 35.4" 620 x 710 x 900 mm 19.3" x 20.6" x 26.2" 490 x 523 x 665 mm 15 max / 4 supplied std. 17.7" x 17.7" (15.4 lbs capacity) 450 x 450 mm (7 kg capacity) 205 lbs 93 kg MCO-19M MCO-19M(UV) MCO-19M(UVH) L 24.4" x 27.9" x 35.4" 620 x 710 x 900 mm 19.3" x 20.6" x 26.2" 490 x 523 x 665 mm 15 max / 3 supplied std. 17.7" x 17.7" (15.4 lbs capacity) 450 x 450 mm (7 kg capacity) 207 lbs 94 kg FRONT SIDE 4.5" (115 mm) 24.4" (620 mm) 19.3" (490 mm) 24.8" (630 mm) 20.6" (523 mm) 3.1" (80 mm) 1.9" (49 mm) 1.3" (34 mm) 34.6" (880 mm) 26.2" (665 mm) 4.9" (125 mm) 22.8" (580 mm) 0.8" (20 mm) 18.4" (469 mm) 1.7" (44 mm) 11

12 Sterisonic GxP CO 2 Series Specifications Sterisonic GxP CO 2 Incubators Description Single Chamber Model Number MCO-19AIC(UVH) MCO-19AIC(UV) MCO-19AIC Dual Chamber Model Number MCO-38AIC(UVH) MCO-38AIC(UV) MCO-38AIC Major Operating Systems H2O2 Sterilization System Standard Optional Optional SafeCell UV System Standard Standard Optional Single Beam, Dual Detector IR CO2 Sensor Standard Standard Standard incu safe Copper Enriched Stainless Steel Interior Standard Standard Standard LCD Graphical Controller/Display, Door Mounted Standard Standard Standard Direct Heat, Air (DHA) Air Jacket Standard Standard Standard Sterilization and Decontamination H2O2 Sterilization System Vaporization in situ Optional Optional Interior UV Lamp, Programmable, Ozone Free Standard Standard Optional Copper Enriched Stainless Steel Interior with Germicidal Protection Standard Standard Standard Environmental Performance Temperature Control Range +5ºC above ambient to 50 C (in a 5ºC to 35ºC ambient) Temperature Control Uniformity Deviation CO2 Control Range and Deviation CO2 Sensor Platform CO2 Calibration Airflow Interior Humidity ±0.25ºC (in 25ºC ambient, setting 37ºC, 5% CO2, no load) 0% to 20%, ±0.15% in 25ºC ambient, setting 37ºC, 5% CO2, no load Ceramic based, single beam, dual wavelength measurement of actual vs. contrast, with continuous auto-zero calibration. Single point zero automatic; semi-automatic one point reference optional. Gentle vertical airflow, continuous with inner door closed. 37 C through evaporation via DHA heating system; reflective optical low water sensor. Control, Monitoring, Alarm Temperature and CO2 Control Display Data Acquisition Communications P.I.D., setpoint resolution 0.1% and 0.1 C Alphanumeric LCD digital display messaging. Data Acquisition Automatic log function of temperature and CO2. Remote alarm contacts standard. Optional 4-20mA connection. Optional PC interface, Catalog No. MTR-480 with RS232/RS485 data ports available Cabinet Design and Construction Interior, Shelves Inner Door Insulation Outer Door Access Port Leveling Feet 4, Copper-enriched stainless steel. Tempered glass. Rigid foam polyurethane. Reversible, heated. Single opening with interior and exterior 1.18" (30 mm) non-voc silicone stoppers. 4, adjustable. 12 Sterisonic GxP Series Cell Culture Incubators

13 Sterisonic GxP CO 2 /O 2 Series Specifications Sterisonic GxP CO 2 /O 2 Incubators Description Single Chamber Model Number MCO-19M(UVH) MCO-19M(UV) MCO-19M Dual Chamber Model Number MCO-38M(UVH) MCO-38M(UV) MCO-38M Major Operating Systems H2O2 Sterilization System Standard Optional Optional SafeCell UV System Standard Standard Optional Single Beam, Dual Detector IR CO2 Sensor Standard Standard Standard incu safe Copper Enriched Stainless Steel Interior Standard Standard Standard LCD Graphical Controller/Display, Door Mounted Standard Standard Standard Direct Heat, Air (DHA) Air Jacket Standard Standard Standard Sterilization and Decontamination H2O2 Sterilization System Vaporization in situ Optional Optional Interior UV Lamp, Programmable, Ozone Free Standard Standard Optional Copper Enriched Stainless Steel Interior with Germicidal Protection Standard Standard Standard Environmental Performance Temperature Control Range +5ºC above ambient to 50 C (in a 5ºC to 35ºC ambient) Temperature Control Uniformity Deviation CO2 Control Range and Deviation CO2 Sensor Platform CO2 Calibration O 2 Control Range and Deviation O 2 Sensor Platform Airflow Interior Humidity ±0.25ºC (in 25ºC ambient, setting 37ºC, 5% CO2, no load) 0% to 20%, ±0.15% in 25ºC ambient, setting 37ºC, 5% CO2, no load Ceramic based, single beam, dual wavelength measurement of actual vs. contrast, with continuous auto-zero calibration. Single point zero automatic; semi-automatic one point span at reference (optional). 1-18%, 22-80%, ±0.2% in 25ºC ambient, setting 37ºC, no load Zirconia sensor, microprocessor P.I.D. control Gentle vertical airflow, continuous with inner door closed. 37 C through evaporation via DHA heating system; reflective optical low water sensor. Control, Monitoring, Alarm Temperature and CO2 Control Display Data Acquisition Communications P.I.D., setpoint resolution 0.1% and 0.1 C Alphanumeric LCD digital display messaging. Data Acquisition Automatic log function of temperature and CO2. Remote alarm contacts standard. Optional 4-20mA connection. Optional PC interface, Catalog No. MTR-480 with RS232/RS485 data ports available Cabinet Design and Construction Interior, Shelves Inner Door Insulation Outer Door Access Port Leveling Feet 3, Copper-enriched stainless steel. 4 separate, gasketed inner doors, tempered glass Rigid foam polyurethane. Reversible, heated. Single opening with interior and exterior 1.18" (30 mm) non-voc silicone stoppers. 4, adjustable. 13

14 Sterisonic GxP CO 2 Series Accessories SANYO Biomedical products include a broad range of accessories to meet specific application requirements. For accessories or options not listed herein, contact SANYO or your authorized SANYO sales representative. Sterisonic GxP CO 2 Series Incubators Single Chamber Model Number Dual Chamber Model Number MCO-19AIC(UVH) MCO-38AIC(UVH) MCO-19AIC(UV) MCO-38AIC(UV) MCO-19AIC MCO-38AIC Catalog No. Catalog No. Catalog No. H2O2 Sterilization Kit Built-in MCO-HL N / A H2O2 Vapor Atomizer MCO-HP N/A N / A H2O2 Reagent (Formulated for SANYO Sterisonic GxP) MCO-H2O2 N/A N / A Automatic CO2 Cylinder Switchover System MCO-21GC MCO-21GC MCO-21GC Gas Calibration System, semi-automatic one point calibration function. MCO-SG MCO-SG MCO-SG CO2 Cylinder Regulator, CGA fitting 320 MCO-100L MCO-100L MCO-100L Roller Base. For use in single or stacked installations. MCO-18RB MCO-18RB MCO-18RB incu safe Shelf and Brackets. Includes two shelf brackets. Full shelf MCO-47ST MCO-47ST MCO-47ST incu safe Half Tray System MCO-25ST MCO-25ST MCO-25ST Integrated Cooling Option MCO-CL MCO-CL MCO-CL Communications Port. Located at rear of chamber. Connector, cable and software not supplied. MTR-480 MTR-480 MTR-480 Communications Port. Located at rear of chamber, analog 4-20mA. MCO-420MA MCO-420MA MCO-420MA SafeCell UV System Kit Narrow-bandwidth 253.7nm lamp and assembly. Built-In Built-In MCO-19UVS H 2O 2 Reagent (MCO-H2O2) SANYO H 2O 2 solution is specially formulated for optimal use with the MCO-HP atomizer. Each pre-measured bottle is sufficient for a complete H 2O 2 sterilization sequence. Unit of issue: six per carton. H 2O 2 Vapor Atomizer (MCO-HP) Shown with connecting cable, standard. Integrated Cooling Coil (MCO-CL) Factory installed; specify when ordering. Water bath/ circulator not included. Permits stable operation at ambient or below ambient temperatures. Extends performance specifications as follows: Temperature Range: +18 C to +50 C, distribution +/-0.25 C, variation +/-0.1 C. Relative Humidity: 5 C to +50 C, 95% +/- 5%RH; 20 C to 25 C >80%RH; 18 C >70%RH Includes temperature mapping results for individual unit per serial number. Relative Temperature Performance +50ºC With Cooling Coil +18ºC +50ºC Without Cooling Coil +29ºC over Ambient Ambient Temperature +24ºC 14 Sterisonic GxP Series Cell Culture Incubators

15 Sterisonic GxP CO 2 /O 2 Series Accessories Sterisonic GxP CO 2 /O 2 Series Incubators Single Chamber Model Number Dual Chamber Model Number MCO-19M(UVH MCO-38M(UVH) MCO-19M(UV) MCO-38M(UV) MCO-19M MCO-38M Catalog No. Catalog No. Catalog No. H2O2 Sterilization Kit Built-in N/A N / A H2O2 Vapor Atomizer MCO-HP N/A N / A H2O2 Reagent (Formulated for SANYO Sterisonic GxP) MCO-H2O2 MCO-H2O2 N / A Automatic CO2 Cylinder Switchover System MCO-21GC MCO-21GC MCO-21GC Gas Calibration System, semi-automatic one point calibration function. MCO-SG MCO-SG MCO-SG CO2 Cylinder Regulator, CGA fitting 320 MCO-100L MCO-100L MCO-100L N2 Cylinder Regulator, CGA fitting 580 (for low oxygen applications) MCO-100N MCO-100N MCO-100N O2 Cylinder Regulator, CGA fitting 540 (for high oxygen applications) MCO-100M MCO-100M MCO-100M Roller Base. For use in single or stacked installations. MCO-18RB MCO-18RB MCO-18RB incu safe Shelf and Brackets. Includes two shelf brackets. Full shelf MCO-47ST MCO-47ST MCO-47ST incu safe Half Tray System MCO-25ST MCO-25ST MCO-25ST Integrated Cooling Option MCO-CL MCO-CL MCO-CL Communications Port. Located at rear of chamber. Connector, cable and software not supplied. MTR-480 MTR-480 MTR-480 Communications Port. Located at rear of chamber, analog 4-20mA. MCO-420MA MCO-420MA MCO-420MA SafeCell UV System Kit Narrow-bandwidth 253.7nm lamp and assembly. Built-In Built-In MCO-19UVS Integrated Cooling Option Feature Temperature Range Relative Humidity Components +18ºC to +50ºC, distribution +/-0.25ºC, variation +/-0.1ºC 5ºC to +50ºC, 95% +/- 5%RH; 20ºC to 25ºC > 80%RH; 18ºC > 70%RH Includes temperature mapping results for individual unit per serial number Stainless Steel Cooling Coil, Interconnection Lines, 6 L, Refrigerated Water Bath & Circulator (Optional/Not Provided) 15

16 Service and Technical Support Unique SANYO Services On-site consultation Specialized documentation for each individual unit Customized testing procedures based on personalized customer requirements SANYO Connect SANYO s customer-driven biomedical service program guarantees local attention from qualified SANYO service representatives, whenever and wherever you need it. New Unit Installation and Training Preventative Maintenance Warranty and Non-Warranty Repairs Calibration/Validation Services Refurbishment and Reconditioning Customized Service and Warranty Programs In-Stock Parts for Immediate Delivery Predelivery and On-Site Services Predelivery services include factory acceptance testing, calibration, and temperature mapping. On-site services include installation qualification, operational qualification, performance qualification, calibration and temperature mapping. Product conforms to RoHS (European Restriction of Hazardous Substance directives) SANYO Electric Co.,Ltd., Biomedical Division, Gumma is certified for quality management system:iso9001/medical devices quality management system:iso13485/environmental management system:iso14001 SafeCell UV U.S. Patent ; Direct Heat and Air Jacket U.S. Patent ; SafeCell UV, incu safe, Direct Heat and Air Jacket, P.I.D./R and Active Background Contamination Control, are trademarks of SANYO Electric Biomedical Co., Ltd Specifications subject to change without notice. SANYO North America Corporation Biomedical Solutions Division 1300 Michael Drive, Suite A, Wood Dale, IL Toll free USA (800) , Fax (630) V6

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