Laboratory Safety Audit
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1 Laboratory Safety Audit Principal Investigator: Building: Room #: Auditor: Date: Lab Safety Plan 1. Lab safety plan available 2. Plan reviewed/evaluated annually 3. Current Lab Safety Guide available 4. Personnel page is up-to-date 5. Hazard profile is up-to-date 6. Standard Operating Procedures available 7. Lab safety Plan is supplemented with BL2 information 8. Specifc biotoxin training completed & documented 9. Lab Safety Plan coveres laser operation, alignment, & policies 10. Annual BL2 training completed 11. Annual laser training completed 12. Training provided/documented 13. Current exposure control plan available 14. Signed statements for Hep. B on file 15. Safety Data Sheets available 16. All lasers are registered with DRS Personal Protective Equipment (PPE) 1. Appropriate gloves available 2. Eye/face protection available 3. Appropriate lab coats available 4. PPE is being utilized appropriately 5. Respirator users fit-tested annually 6. Lab coats decontaminated 7. Adequate laser eye protection available 8. Laser eye protection is in good condition 9. Laser eye protection worn inside Nominal Hazard Zone 10. Laser eyewear inspection documented 11. Adequate PPE avalable for Class 4 lasers
2 Laboratory Safety Audit 2 Laboratory Housekeeping 1. No eating or drinking 2. No clutter 3. No un-cleaned spills or residues 4. Aisles and exits 28 wide and unobstructed clearance around sprinklers 6. Soap and paper towels readily available 7. Floors and work surfaces can be cleaned & decontaminated 8. Work surfaces routinely disinfected 9. Effective pest management program 10. No animals/plants not associated with work Electrical 1. Extension cord use is temporary 2. Proper grounding is used 3. Cord and equipment in good condition 4. No outlet overloading 5. Electrical panels accessible 6. Outlets near water GFCI protected 7. Shock hazards have proper signage Engineering Controls 1. Chemical fume hood intact & functioning properly 2. Chemical fume hood being used correctly 3. Biosafety cabinet present & in an adequate location 4. Biosafety cabinet certification up-to-date 5. Shut-off valve for gas line external to biosafety cabinet 6. Proper engineering controls/ventilation is utilized 7. Machine guards in place
3 Laboratory Safety Audit 3 Other Equipment 1. Chairs easily decontaminated (BL1 & BL2) 2. Vacuum protected with traps 3. Vacuum exhaust vented properly 4. Vacuum glassware coated 5. Tubing in good condition 6. Equipment properly secured 7. No mercury thermometers in ovens 8. Sink for hand-washing present in lab Work Practices 1. Needles are not tampered with 2. Safety cups or o-ringed tubes available & used properly 3. Autoclave available 4. Controlled access to labs 5. A "Toxins in Use- Authorized Personnel Only" sign is present Emergency Equipment 1. Fire Extinguishers: a. Correct type present b. Readily accessible c. Checked monthly & documented Adequate emergency equipment available Safety Showers in unobstructed and unaltered Eyewashes: a. Unobstructed & properly maintained b. Activated weekly & documented Chemical spill kit stocked with appropriate materials Tools to pick up broken glass are readily accessible Spill kit is readily accessible Calcium gluconate available for HF Storage and Transport 1. Per chemical compatibility 2. Upright and safe storage 3. Appropriate containers
4 Laboratory Safety Audit Containers labeled Containers closed Secondary containment used where necessary Appliances identified for lab use Condition/quality of chemical is good Inventory for Risk Group 2 biotoxins Risk Group 2 samples stored in secure location Risk group 2 materials segregated from other materials Risk Group 2 materials properly labeled and have BH symbol Waste 1. Container in good condition 2. Containers labeled 3. Containers closed 4. Waste streams segregated 5. Secondary containment used 6. No waste accumulation 7. Glass disposed of properly 8. Sharps disposed of properly 9. Biohazard container lidded, leak proof & labeled with BH symbol Compressed Gases Present? Y N 1. Cylinders secured 2. Tubing poses no hazard 3. Away from heat 4. Flammable and Oxidizing gases separated. 5. Total number within limit 6. Tubing & plumbing compatible with gas 7. Away from exits 8. Toxic Gases in enclosures Cryogens Present? Y N 1. Adequate room ventilation 2. Appropriate containers 3. Away from combustibles
5 Laboratory Safety Audit 5 Flammable Liquids Present? Y N 1. Amount within limit 2. Away from ignition sources 3. Bonding & Grounding used for bulk vessels 4. Certified refrigerators used Peroxide Forming Chemicals Present? Y N 1. Peroxide formers dated 2. Tested regularly & documented Pyrophoric Materials Present? Y N 1. Gas cylinders secured in enclosure 2. Tubing & plumbing compatible 3. Away from exits 4. Sprinkler system present 5. Flame resistant lab coats available Explosive Materials Present? Y N 1. Material that becomes explosive when dry is checked 2. Proper documentation is provided for handling explosives. 3. Materials are stored according to standards. Lasers Present? Y N 1. Controls to minimize fire potential sufficient for laser use 2. Safety interlocks perform as intended for lasers 3. Protective housing in place 4. Laser mounted on optical bench or other stable platform 5. Laser beam not at eye level 6. Laser beam control is adequate Laser warning system available (visual or aural) Entryway control is present (interlock or blocking barrier) Emergency disconnect switch available for lasers Area with lasers is appropriately posted All lasers and protective barriers are labeled
6 Laboratory Safety Audit 6 Radioactive Materials Present? Y N 1. General Practices a. Radioisotope records are current and complete b. Radioactive materials secured or attended c. Radioactive material is used/stored in authorized location d. Radioactive material is properly labeled e. Radioactive material areas & equipment properly labeled f. Radioactive waste is properly stored g. Proper shielding materials and handling devices utilized h. Absorbent paper utilized and in good condition 2. Surveys a. Contamination surveys completed within 7 days of work b. Contamination surveys utilize correct technique c. Findings of elevated contamination addressed d. Survey intrumentation available within last 12 months 3. Training a. Users of radioactive material have current training b. Non-users have current awareness training
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