THE EFFECT EVEN IN THE FIRST YIELD

Transcription

1 THE EFFECT EVEN IN THE FIRST YIELD 20%* guaranteed yield increase 22%* sugar BRX increase 100%* resistance increase 100%* more carbon in soil 200%* increase in phosphorus assimilability Up to 95%* limitation of the occurrence of fungi and root tumours 50%* limitation of application of mineral fertilizers 50%* reduction of application of crop protection chemicals

2 100% natural fertilizer (WE) which stimulates the plants growth and increases their resistance TriPLUS Aloe Vera is composed in 80% of organic aloe which has natural phytochemical compounds and polysaccharides of plant origin which are essential for plants and enrichment of soil fertility. It contains nutrients which are necessary for the plant growth such as functional microorganisms, combinations of bacteria and fungi which improve the quality and richness of soil and also contribute to the plant growth. The product contains also a complete nutritional composition in the form of macro- and micronutrients, amino acids and vitamins. The preparation contains most of all, all known due to their beneficial properties, substances included in the aloe due to which it strengthens plants and makes them more resistant and less sensitive. TriPLUS Aloe Vera contains microelements (trace elements), amino acids, PGR compounds, enzymes and active compounds (aloin, barbaloin etc.) what makes up natural antibiotic protection against pathogens. It is enriched with mycotic microorganisms acting in soil which ensure nitrogen through the colonization of soil and root tissue, phosphate solubilisation and supply of PGPR (plant growth-promoting bacteria). It contains enriched humic acids in order to increase the abilities of soil solidification and improve the effectiveness of the application of non-organic fertilizers.

3 FIELD RESULTS examples of TriPLUS Aloe Vera performance ONION - CHIVES Parameters TriPLUS Aloe Vera Control Area 1 ha 1 ha Variety Local Local Fertilizer MINERAL TRIPLUS Spraying Standard in general 15 l divided into the number of cycles Every 7-10 days cycle Standard Yield 16,000 kg 9,000 kg Onion cultivation fertilization with TriPLUS Aloe Vera It is similar with garlic cultivation subject to processes TriPLUS Aloe Vera Fertilized in a standard manner

4 FIELD RESULTS examples of TriPLUS Aloe Vera performance TOMATO Parameter TriPLUS Aloe Vera Control Area 1 ha 1 ha Variety Local Local Fertilizers Mineral Pesticides Manure TriPLUST Aloe Vera 50% of dose 50% of dose 20,000 kg 20 l Atomizing Spraying every 7 days in 4 doses - Standard Standard 20,000 kg - Yield 94,000 kg 70,000 kg Sister plants from the same seeding, on the right the result with TriPLUS Aloe Vera and on the left the result without it. Seeds wetting plus spraying.

5 FIELD RESULTS examples of TriPLUS performance BEANS, AUBERGINE, TOMATO Crop BEANS Crop yield (I) Crop yield (II) Crop yield (III) AUBERGINE Crop yield (I) Crop yield (II) Crop yield (III) TOMATO Crop yield (I) Crop yield (II) Crop yield (III) Fertilization (kg) Manure + Triplus Manure + NPK Only manure Spraying Quality Every 7-10 days a portion of a dose depending on the number of cycles. Maximum 20 litres per hectare per season. More durable while storing, fresher look, higher calorific value and sugar content, essentially increased resistance to freezing.

6 FIELD RESULTS examples of TriPLUS performance CUCUMBER Parameter TriPLUS Aloe Vera Control Area 1 ha 1 ha Variety Local Local Fertilizers Mineral Pesticides Manure TriPLUS Aloe Vera 50% of dose 50% of dose 20,000 kg 20 l Rozpylający Spraying every 7 days in 4 doses - Standard Standard 20,000 kg - Plon 60,000 kg 40,000 kg

7 FIELD RESULTS examples of TriPLUS performance WHEAT Parameter TriPLUS Aloe Vera Control Area 1 ha 1 ha Variaty Dickens Dickens Fertilizers Mineral Pesticides TriPLUS Aloe Vera 50% of dose 50% of dose 13 l Atomizing Spraying every 10 days in 4 doses - Standard Standard - Yield 12,400 kg 9,300 kg

8 FIELD RESULTS examples of TriPLUS performance BEETROOT Parameter TriPLUS Aloe Vera Control Area 1 ha 1 ha Variety Local Local Fertilizers Mineral Pesticides Manure Triplus Aloe Vera 50% of dose 50% of dose 20,000 kg 20 l Atomizing Spraying every 7 days in 4 doses - Sugar BRIX 22% increase 17% standard standard 20,000 kg - Yield 92,000 kg 70,000 kg

9 THE INCREASE OF PLANTS RESISTANCE Foliar application of TriPLUS Aloe Vera leads to the increase of plant resistance. TriPLUS Aloe Vera causes higher yield due to its composition and ensures crops of higher nutritional value. Most aloe components act also like a perfect carrier for beneficial bacteria and other additional natural components which as a consequence of the production process are included in the preparation formula. The plant root system achieves very efficient stimulation and very high absorption speed due to which it sucks the available beneficial nutrients. With the use of the preparation, the plant root system is subject to very efficient stimulation which causes growth of assimilation of nutrients available in soil. Without TriPLUS Aloe Vera application of TriPLUS Aloe Vera Aloe is known for its unique properties. When applied in plant cultivation, it limits the development of pathogens and many diseases. It fights root tumours in 95%. The mechanism has been already described in the case of applying aloe in unconventional and aesthetic medicine. Even many years ago, people were aware of beneficial effects of using various aloe extracts, both in nutrition and in prevention of diseases.

10 YIELD economic justification of TriPLUS application THE INCREASE OF RICE PROFITABILITY Fertilization (tonnes) Percentage yield increase Conventional (400 kg fertilizer NPK/ha) Fertilization with 50% NPK and 50% Triplus % Fertilization with 100% NPK and 100 Triplus % Photographs presenting rice cultivation after applying TriPLUS Aloe Vera and NPK fertilizers Parameters Average per group I II III Yield (kg) 0% Triplus + 50% Triplus + 100% Triplus + 100% NPK 50% NPK 100% NPK Number of groups 132 groups 132 groups 132 groups Yield 6.45 tonnes/ha 7.08 tonnes/ha tonnes/ha

11 SOIL RECULTIVATION Significantly increased moisture retention observed in the treated areas as it was shown on the photographs above. Soil in the farmhouse is mostly sandy and clay and with time it became relatively unstructured and it was difficult to maintain moisture. The soil usually dries to dust in dry periods and makes up the unstructured clay in wet periods. During the last three years of TriPLUS Aloe Vera application, a sudden growth of the soil structure was observed with simultaneous growth of plant roots development and increase of the level of measured organic carbon. Clear benefits achieved as a result of the tests (see table below) became the catalysts for accepting the below scheme of nutrients in connection with the selected products which improve soil in the whole object during the vegetation season The last extensive tests carried out on soils showed significant improvement of soil biology, especially with regard to the obtained level of carbon and phosphorus content. The results are marked in the tables on the next page.

12 The availability of phosphorus for plant roots is limited in acidic and alkali soils mostly as a result of creation of sparingly soluble phosphate compounds with aluminium (Al) and iron (Fe) in acidic and with calcium (Ca) in alkali soils. Every year farmers spread large amounts of phosphorus fertilizer in soils intended for production of food, fibres and wood and only 10-20% of it may be absorbed by plants. The remaining portion quickly transforms into unavailable forms of phosphorus which are not absorbed by plant roots. The application of products for formation of microorganisms such as TriPLUS Aloe Vera will stimulate the plant root system and enzymatic activity connected with acidic or alkali phosphates. It increases the availability of nutritious phosphates for plants in soil and improves the plant functioning Attention: the results are supported with the report of 2005 on NSA case Microbes & Minerals published by NSW in 2005: Formation of organic soil matter helps building up the organic pool of phosphorus

13 The soil samples collected at the end of the late winter showed strong increase of the level of available phosphorus despite the low rainfalls and cold weather. The higher availability of nutrients reflects significant increase of organic matter content in the soil and in the fractions of total carbon. The total carbon increase was higher than 1.3%* in the zones subject to the performance of TriPLUS Aloe Vera in the cultivation period. The change of the soil structure in a relatively short period is a very encouraging result with regard to the application of TriPLUS Aloe Vera, when the soil structure treated with the substance was compared with the untreated one (see photographs below). Examples of improved soil structure and moisture retention in the fertilized cultivation. In comparison to the untreated barley crops. Higher levels of the organic carbon content transformed the previously unfertilized clay. Higher nutrients level was supplied to the plants by the significantly improved root system.

14 After the application of TriPLUS Aloe Vera it can be noticed with a naked eye that the fertilized soil is darker and that is has more water. Nutrients and carbon in the humus are visible compared to the unfertilized soil which is lighter and has a lighter colour. Also, the size of the root system because of the root growth and their development is much greater in a plant treated with TriPLUS Aloe Vera. It influences on the content of starch, protein, sugar, pectin and fat in plants. Sugar BRIX value after the application of TriPLUS Aloe Vera increased to 11.8%* in comparison to 5.8% on the untreated area. It states about higher level of nutrients.

15 RESULTS The total production size was much higher in areas subject to TriPLUS Aloe Vera. The result is very encouraging taking into account the disadvantageous growth conditions present in the period. The costs of introducing the fertilizer were smaller than half of expenditures related to mineral fertilizers. After the application of TriPLUS Aloe Vera a significant growth of the organic matter content and improvement of organic carbon content in the soil was noticed. Moisture retention in sprayed areas was also observed despite the tough drought in the farmhouse. A significant improvement of available phosphorus in the fertilized areas was observed. It especially referred to the region in which the farmhouse was located, where it is usually difficult to maintain the level of available phosphorus. The improvement of the soil structure and fragility was also noticed. It reflects the increasing amount of organic carbon measured in the fertilized soil in the cultivation period. The twin plants, on the left the one untreated with TriPLUS Aloe Vera and on the right the plant subject to full growth stimulation process using TriPLUS Aloe Vera. TriPLUS Aloe Vera is a great natural repellent BIRD AND INSECT REPELLENT TriPLUS Aloe Vera acts mostly as a fertilizer and stimulator which supports the plants development. The reduced interest of birds and insects (vermins) was also shown. The attacks on the crops decreased because of the amount of natural aloin in AloeVera Triplus. The application of AloeVera Triplus is a great and environmentally friendly way of natural limitation of the attacks of bird and insect vermins.

16 Directions for use / application / dosage ATTENTION : Mix THOROUGHLY before use so that no sediment was left at the bottom of the container. Mix the solution of TriPLUS Aloe Vera immediately after opening and do not leave the solution premixture for a longer period of time. Do not spray on the plants during the pollination phase. Wet the roots in the transplanting phase. Sprinkle the whole surface of leaves as well as underneath, if possible. After preparing the ground, make openings for seeding and fertilizing using a standard fertilizer (fertilizer or compost) and then spray water with the solution of TriPLUS Aloe Vera (200:1 ratio). Sprinkle in open areas in the mornings or in the evenings (temperature up to 25 C). The objective is to minimize the dependency from the artificial fertilizers and to obtain better yield with smaller expenditures due to the application of the new TriPLUS Aloe Vera fertilizer. The reduction of artificial fertilizers to 50% using 50% of Aloe Vera fertilizers (depending on the type of plants, soil quality, etc.) is realistic. Remember that each year the quality of your soil and its fertility will improve and ensure greater yield due to the regular application of TriPLUS Aloe Vera fertilizers. The planting procedures as well as the application of TriPLUS Aloe Vera may differ in some situations, this is why we are asking you to contact with our local representative in order to obtain further technical support or with the company headquarters on the phone. We remain at your disposal.

17 DIRECTIONS FOR USE: TriPLUS Aloe Vera - is a foliar and soil-applied fertilizer to be used in the form of water solution in agricultural, fruit-growing, vegetable and ornamental plants cultivation which contains microelements soluble in water. TriPLUS Aloe Vera significantly increases and improves: - tolerance to some adverse atmospheric conditions and some pathogenic factors, - resistance of crops to stress connected with adverse cultivation conditions and poor soil conditions, - yielding and improves the quality of crops, - assimilation and use of nutrients by plants. DOSE AND TIME OF USE: Mix the packaging contents thoroughly before use. Avoid using during intensive insolation or at temperatures above 25 C and strong winds. The procedures shall be carried out in the morning or in the evening. Apply only in justified cases. Do not exceed the recommended doses. Cover the whole leaf area with the solution. Do not apply in the blooming period. Recommendations for various types of plants: Field-scale crops, including maize, rape: Recommended dose: 2-3 l/ha of crops, repeating the procedures every 10 days. Recommended dose for the prepared and weed-free place: 4-10 l/ha, depending on the soil quality. Higher doses shall be applied in the intensive cereal production. The total fertilizer dose shall not exceed 30 l/ha of crops. Water amount: min. 400 l/ha. The first procedure shall be carried out after performing the chemical weed control activities in the crops. In the case of winter crops, when the weeds were controlled in autumn, the procedure shall take place after the visible starting up of vegetation. Fruit-growing and berry bushes, including grapes: After planting, use for foliar spraying in the dose of 2-3 l/ha. The procedures shall be carried out every days. Depending on the planting density, the total dose of the fertilizer shall not exceed 50 l/ha. Water amount: min. 300 l/ha. When applying in irrigation systems, do not exceed the single dose of 10 l/ha. For the purposes of wetting the seedling roots before planting, apply 25% solution. Wet roots for 4 to 6 hours. Plant as soon as possible so that the roots did not get dry.

18 Vegetable crops such as: (TOMATOES, SPINACH, PEPPER, CARROTS, ZUCCHINI, AUBERGINE, RADISHES, LETTUCE, CE- LERY, ONION, LEEK, CABBAGE) Apply fertilizer on the prepared and weed-free places in the dose of 4-6 l/ha. Starting from the first pair of leaves, apply in the dose of 2-3 l/ha. The procedure shall be repeated every 7-10 days. In the event of applying seedlings, perform the first procedure 10 days after planting the seedling. Water amount: min. 300 l/ha. The total fertilizer dose shall not exceed 30 l/ha. For wetting seeds apply in 2% concentration for 2 to 4 hours. Seed all the wetted seeds. Do not store wetted seeds. Potatoes and beetroots: Before the emergence of plants apply fertilizer in the dose of 4 l/ha on the weed-free places. Starting from the emergence of plants, apply it in the dose of 2 l/ha. Repeat the procedures every 7-10 days. Depending on the crops intensity, perform up to 10 procedures. Water amount: min. 300 l/ha. The total fertilizer dose shall not exceed 30 l/ha. For the purposes of wetting or spraying the bulbs before planting apply 2% solution. Grasslands: Before starting vegetation, apply fertilizer in the dose of 3-6 l/ha. Repeat the procedure after every harvest. Recommended water dose: 300 l/ha. Ornamental plants: In cultivation of ornamental plants, for the purposes of watering and spraying apply the working liquid with the concentration of % every days. In the recommended dose, the fertilizer may be used also in the cultivation of succulents. In hydroponic farming do not exceed the dose of 1 litre of fertilizer per 200 litres of nutrient. It may be mixed with other fertilizers applied in hydroponics, however it is always necessary to observe the nutrition recommendations for the cultivated plants. PREPARATION OF USEFUL LIQUID: ATTENTION! IMPORTANT: while preparing the fertilizer solution, proceed as follows: Fill half of the sprinkler s container. Pour the measured and well mixed amount of fertilizer. Make up the sprinkler s container with water. Do not store the prepared for use solution. VERY IMPORTANT: MIX THOROUGHLY before use so that no sediment was left on the bottom of the container. In order to achieve the full effect, it is not recommended to mix the fertilizer with insecticides.

19 STORAGE: TriPLUS Aloe Vera fertilizer shall be stored in a dry and enshadowed place, at the temperature from 5 C to +35 C in an original, tightly closed packaging. Store in the place which is not accessible for children and away from food products and feed for animals, in well ventilated rooms. BEFORE USING THE PRODUCT READ THE LABEL THOROUGHLY. SAFETY PRECAUTIONS: P102 Keep away from children. P103 Read the label before use. Best before: 3 years from the production date Batch number / production date: on the packaging.

20 DETAILED TERMS OF USE FOR TriPLUS Aloe Vera AGENT FOR THE SELECTED CROPS The sum of the fertilizer used for all stages should not exceed 20 litres per hectare. It is necessary to divide the planned fertilizer volume by the number of stages of its use. In the case of very poor soil quality, carry out a field test with doubled amount of the fertilizer. Do not forget that we are dealing with calcium fertilizer which is a very strong growth stimulator and it strongly accelerates the vegetation of plants. MAIZE, WHEAT AND OTHER CEREAL Plant stage / age Ratio Time Application 1. Seed preparation 50:1 3 hours Wetting in the solution 2. Planting stage 300:1 Before planting - seeding Watering or spraying of openings or rows 3. Plant 200:1 Every day Spraying of the whole plants LEAFY VEGETABLES: SPINACH, KALE, BROCCOLI, CABBAGE, CAULIFLOWER, CELERY Plant stage / age Ratio Time Application 1. Seed preparation 50:1 3 hours Wetting in the solution 2. Seedling preparation 300:1 Base mixing Watering or spraying 3. Seedlings 300:1 Every week Seedling spraying or wetting 4. Planting stage 300:1 Before planting - seeding Watering or spraying of openings or rows 5. Plant 200:1 Every 7-10 days Spraying of the whole plants TOMATO, AUBERGINE, PEPPER, WATERMELON, MELON, CUCUMBER, STRAWBERRIES Plant stage / age Ratio Time Application 1. Seed preparation 50:1 3 hours Wetting in the solution 2. Seedling preparation 300:1 Base mixing Watering or spraying 3. Seedlings 300:1 Every week Seedling spraying or wetting 4. Planting stage 300:1 Before planting - seeding Watering or spraying of openings or rows 5. Plant 200:1 Every 7-10 days Spraying of the whole plants

21 ONION, GARLIC, CARROT, BEETROOT Plant stage / age Ratio Time Application 1. Seed preparation 50:1 3 hours Wetting in the solution 2. Seedling preparation 300:1 Before planting Watering or spraying 3. Seedlings 200:1 Every second week Seedling spraying or wetting POTATO Plant stage / age Ratio Time Application 1. Seed preparation 50:1 3 hours Wetting in the solution 2. Seedling preparation 300:1 Before planting Watering or spraying 3. Seedlings 200:1 Every second week Spraying FRUIT: APPLES, SOUR CHERRIES, ETC. Etap rośliny / wiek Stosunek Czas Aplikowanie 1. Seed preparation 50:1 3 hours Wetting in the solution 2. Seedling preparation 100:1 Before planting Immerse the seedlings or the bottom part of the stem in the solution 3. Seedling 200:1 Every 1-2 weeks Seedling spraying or wetting 4. Planting 300: days before planting Fruit watering or spraying 5. Immature plants 300:1 Every 2 weeks Spray on the whole plants or connect irrigation system to the root zone 6. Mature plants 300:1 Every month Spray on the whole plants or connect irrigation system to the root zone

22 ORNAMENTAL PLANTS, FLOWERS Plant stage / age Ratio Time Application 1. Seed preparation 50:1 3 hours Wetting in the solution 2. Seedbed preparation 300:1 Base mixing Base wetting or spraying 3. Seedling 300:1 After a week Seedlings spraying or wetting 4. Planting preparation 300:1 Before planting Watering or spraying of openings 5. In the initial growth phase 200:1 Every 1-2 weeks Spraying of leaves and media 6. Developed plants 300:1 Every 4 weeks Spraying of leaves and media GOLF COURSES, STADIUMS, RESORT GRASS, ETC. 1. Prepare 300:1 ratio, spray every 2-4 weeks. COMPOST 1. Prepare 200:1 ratio in a container, add 5% molasses or sugar. 2. Leave the solution covered for 7-10 days, open it and mix every 3 days and cover it again. For 7-10 days the solution may be applied for composting of organic wastes or animal fertilizer. Spray the compost layer litre of Aloe Vera Triplus may be used per 1 tonne of compost material.

23 FERTILIZER S DETAILED COMPOSITION nutrients, acids, microorganisms, fungi, stimulators Aloe contains active components such as amino acids, minerals, vitamins, enzymes, glycoproteins and carbohydrates. Due to high water content, it may be applied in liquid organic fertilizers as a liquid dissolving medium and also a composition of various chemical compounds which may be the nutrients for plants. Various components and chemical compounds which are naturally included in aloe are used for enriching the nutrients and active components for the cultivated plants. Aloe composition Mono polysaccharides (cellulose, glucose mannose, aldopentose), lignin Benefits and functions in the plants and in soil Carbohydrate type which shall break down into the sources of organic carbon which have a very important function in soil, improve soil physical, chemical and biological properties. It is graduated in humic and fulvic acid. It is a very good source of energy for bacteria. N, P, K (macroelements) Macroelements type and basic elements of plant growth, which are needed by plants in large amounts. N - an important chlorophyll component in the process of plant photosynthesis and in the plant vegetation growth. P - a component significant for the synthesis of carbohydrates and creation of energy for plants, breathing, cell division and formation of the generative plant growth phase, it stimulates the roots growth. K - necessary nutrient, which stimulates the early growth, increases protein production, improves effectiveness of water use; it is of general importance for the durability, longevity and resistance to winter and also improves the resistance to diseases and insects. Mg, Ca, (secondary nutrients) Zn, Fe, Mn, Cu, etc. (microelements/trace elements) Amino acids Vitamins (B1, B2, B6, C, niacin, collin, etc.) Enzymes Inositol Barbaloin, aloe, emodin etc Ca - stimulates formation of roots and leaves strengthening the plant structure. Mg - chlorophyll component, it influences on the enzyme synthesis, it stimulates formation of fruit. They are significant trace elements which influence on the vegetative and generative plant growth phase, quality and quantity of production. If they are not available or in insufficient amount, the growth of plants and yield may not reach the optimum level They build up proteins, create plant growth regulators influencing on the health, growth of plants and cells differentiation They are responsible for acceleration of the cells division, coenzyme of various physiological synthesis and plant metabolism, they help in energy transmission. They catalyse various metabolic processes and synthesis in the plants bodies. It influences on the differentiation and formation of tissues. They increase resistance and plant health level, they protect plants from diseases and pathogens

24 TriPLUS Aloe Vera is rich in acids, carbon and other: - humic - halogen - They enrich the organic carbon content in soil and increase the ability to exchange cations in soil. - Humic acid is a nutrient and a good medium for growing functional microorganisms in soil. - It increases the absorption of nutrients by plants as it may bind with more efficient nutrients; it may be absorbed by plants. - It increases soil fertility. It improves soil aeration due to the increase of soil porosity. It in creases cation volume (CEC). Such improvements increase the soil ability to store nutrients, especially microelements. - It makes up a complex with microelements which protect nutrients against leaching cau sed by rain. N, P and K elements are bound in organic form or by microorganisms. Humic acids increase the efficiency of application of chemical (mineral) fertilizers. - Halogen acid has the ability to bind with heavy metals (creating compound chelates), then it settles them and reduces soil toxicity. - It prevents from ph reduction of acidic soils because of continuous application of chemi cal fertilizers, especially when the soil contains a lot of aluminium. Humic acid binds with the aluminium complex as a compound which is hard to dissolve in water (insoluble), so it will not be hydrolyzed and it will not poison the plants. Nutrients N: 1,14% P 2 O 5 : 1.02% K 2 O: 1.03% Fe: 345,23 ppm Mn: ppm Zn: ppm B: ppm Mo < 0.20 ppm Co: < 0.05 ppm Z: ppm Organic carbon: 5.08% - Population of functional microorganisms: Rhizobium (Bradyrhizobium japonicum) Azospirillum sp Bacillus subtilis Aspergillus niger Lactobacillus sp Phosphate Solubilizing Bacteria (Pseudomas putida) 4.8 x 10 x 10 8 cfu/ml 1.2 x 10 8 cfu/ml 6.5 x 10 9 cfu/ml 5.0 x 10 8 cfu/ml 2.7 x 10 9 cfu/ml 7.4 x 10 9 cfu/ml

25 Microorganisms present in Aloe Vera TriPLUS Aloe Vera Group Example Basic microorganisms 1. Free living Azotobacter, Beijerinkia, Clostridium, Klebsiella, Anabaena, Nostoc 2. Symbiotic Rhizobium, Frankia, Anabaena azollae 3. Symbiotic associative Azospirillum Solubilising microorganisms 1. Bacteria Bacillus megaterium var. phosphaticum, Bacillus subtis, Bacilius circulans, Phedomonas striata 2. Fungi Penicillium sp., Aspergilus awamori Mobilizing microorganisms 1. Arbuscular mycorrhiza Glomus sp, Gigaspora sp., Acaulospora sp., Scutellospora sp. & Sclercystis sp. 2. Ectomycorrhiza Laccaria sp., Pisolithus sp., Boletus sp., Amanita sp. 3. Ericoid mycorrhizae Pezizella ericae 4. Orchid mycorrhiza Rhizoctonia solani Nutritious micro-components 1. Silicate and Zinc solubilizers Bacillus sp. Stimulating growth of Rhizobacteria 1. Pseudomonas Pseudomonas fluorescens

26 Description of microorganisms present in TriPLUS Aloe Vera Rhizobium The bacteria are telluric bacteria which may colonize plant roots, and which symbiotically decompose atmospheric nitrogen. They are the most efficient bio-fertilizers expressed per the quantity of determined nitrogen. They occur in seven types and are highly useful for the purposes of formation of root nodule bacteria in plants determined as cross inoculation group. Inoculation with Rhizobium for the last four decades by the American farmers led to the formation of lots of ineffective seedlings in soil the replacement of which using efficient Bradyrhizobium strains became the problem which cannot be overcome. The application of TriPLUS Aloe Vera is the response to it. Azotobacter Among few species of Azotobacter, A. chroococcum is prevailingly dominant in arable lands able to fix N2 (215 mg N2 of constant source of carbon) in the culture media. Azotobacter bacteria produce a lot of water what helps in aggregation and recultivation of soil. The number of A. chroococcum in the examined soils rarely exceeds 105 per a gram of soil because of the lack of organic matter and presence of antagonistic microorganisms in soil. TriPLUS Aloe Vera supplies Azotobacter and causes its multiplication

27 Azospirillum Azospirillum lipoferum and A. brasilense (Spirillum lipoferum in earlier literature) are original soil inhabitants. They ensure associative symbiotic dependency with plants. Bacteria of Azospirillum type are organisms which transform N2 in arable crops. There is Vibrio or Spirillum with the number of polybetahydroxybutyrate accumulation (70%) in cytoplasm. There are five species of Azospirillum described: A. brasilen se, A. lipoferum, A. amazonense, A. halopraeferens and A. irakense. The organism spreads in both, anaerobic and aerobic conditions and it is a beneficial micro-electrolyser in the presence or absence of joined nitrogen in the nutrient. Apart from processing nitrogen, they increase the growth of IAA substances, resistance to diseases and resistance to drought which are only some of additional benefits resulting from the inoculation of Azospirillum. Azolla Azolla is a water fern which floats on the water and collects atmospheric nitrogen together with Anabaena azollae. The leaves of azolla are made of sporophytes of floating rhizomes which overlap each other with cloud-shaped leaves and roots. The areas of their cultivation in the world show increased interest in using the symbiotic Azolla N2 as an alternative source of nitrogen or as an additive to commercial nitrogen fertilizers. Azolla is applied as a bio-preparation and it is known for 4,060 kg of N/ha. In connection with the fact that TriPLUS Aloe Vera contains Azolla, it is possible to limit mineral fertilizers.

28 Phosphate solubilizing microorganisms(psm) Some telluric bacteria and fungi, especially of Pseudomonas, Bacillus, Pénicillium, Aspergillus etc. species secrete organic acids and lower ph in their neighbourhood causing dissolution of bound phosphates in the soil. Increased wheat and potato yield are caused by the inoculation of cultures based on peat, Bacillus polymyxa and Pseudomonas striata. AM fungi The transfer of nutrients, mostly phosphorus and also zinc and sulphur to the cells depends on the intracellular fungal endosymbiont of Glomus, Gigaspora, Acaulospora, Sclerocysts and Endogone species which possess blister-like structures for storing nutrients. The nutrients are absorbed into the root system. Glomus seems to be the most popular type as it has a few species distributed in soil. The availability of pure AM fungi (Arbuscular Mycorrhi za) cultures is an obstacle in a large-scale production despite the fact that the beneficial effects of AM fungi inoculation in plants have been shown many times in the experimental conditions in a laboratory, especially in connection with other nitrogen hardeners. Due to the application of TriPLUS Aloe Vera, the plants are supplied by the fungus hyphae with mineral compounds, mostly phosphorous and nitrogenous ones, although improvement in terms of plants supply in microelements such as zinc and copper was also confirmed.

29 Silicate solubilising bacteria (SSB) The microorganisms are able to decompose silicates and aluminium silicates. During the metabolism of microorganisms some organic acids are formed which have a double role in weathering recultivation processes of soil. They supply H + ions to the substrate and contribute to hydrolysis while organic acids such as citric acid, oxalic acid, Keto acid and carboxylic acids, together with the cations, support their removal and maintenance in the nutrient in a dissolved condition. The examinations carried out on Bacillus sp. isolated from the crusher s granite soil showed that the bacteria are able to dissolve some silicate minerals in in vitro conditions. The examinations on the soil inoculation with a selected isolate with red soil, clayish soil, sand and soil from hills showed that the organisms multiplied in all types of soil and released greater amount of silicates. Joining SSB with the rests additionally led to the increase of the plant growth and seeds yield. The strengthening results from greater solubility of silica and nutrients from soil. Plant growth STIMULATOR Rhizobacteria (PGPR) A group of bacteria which colonize roots or soil is beneficial for plants and is referred to as bacteria responsible for the increase of the plant growth (PGPR). The commercialized at present PGPR modifications contribute to the development through at least one mechanism Suppression of plant diseases (referred to as Bio-protectants), improvement of obtaining nutrients (referred to as Bio-fertilizers) or production of phytohormones (referred to as Bio-stimulants). Pseudomonas and Bacillus species produce also phytohormones referred to as growth regulators, which cause the plants have greater number of small roots, what increases the absorption surface for water and nutrients collection. The PGPRs are referred to as bio-stimulants and phytohormones produced by them cover indoleacetic acid, cytokinins, gibberellins and ethylene production inhibitors. Good competition and high saprophytic competences are the main factors which decide about the success of the bacteria strain as inoculants. * in comparison to control cultivation