Plant Propagation Protocol for Kevin Wilen ESRM 412 Native Plant Production Spring 2008 TAXONOMY. Populus Tremuloides

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ASEXUAL VS. SEXUAL PROPAGATION OF QUAKING ASPEN. Robert B. Campbell, Jr.

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Plant Propagation Protocol for Kevin Wilen ESRM 412 Native Plant Production Spring 2008 Family Names Family Scientific Name: Family Common Name: Populus Tremuloides Quaking Aspen Scientific Names Genus: Cottonwood- Populus L (3) Species: Populus tremuloides (3) TAXONOMY Species Authority: Michx (3) Common Trembling Aspen- P. tremuloides (2) Synonym(s) (include full scientific names Species Code (as per USDA Plants database): Geographical range POTR5 (3) GENERAL INFORMATION Abundant throughout the province of Canada east of the Cascades. Also found in most of Washington except for the Olympic Peninsula, and the sagebrush and subalpine ecosystems. Very sporadic on occurrence. (1) Ecological Climate and elevation range Plant characteristics On low to medium elevation, moist to wet sites; forms extensive stands on islands and floodplains along major rivers and on disturbed upland sites. (2) Elevations of 300m to 1800m. (1) The bark is mostly smooth and white with black horseshoe markings. The leaves have an abrupt tip that are rounded or heart shaped. (1) PROPAGATION DETAILS Propagation Goal Nursery (3) Propagation Method Seed (3) Product Type Propagated by Bare Root Yes Propagated by Bulb Propagated by Container Yes Propagated by Corm Propagated by Cuttings Yes

Time to Grow (from seeding until plants are ready to be outplanted): Target Specifications (size or characteristics of target plants to be produced): Propagule Collection (how, when, etc): Propagule Processing/Propag ule Characteristics Pre-Planting Propagule Treatments Hardening Phase Propagated by Seed Propagated by Sod Propagated by Sprigs Propagated by Tubers (3) -Rapid growth rate -65ft. at maturity. (3) Yes Collect seed from May to mid-june. Collect cottony, wind-borne seeds about a week before capsule opens. Collect lateral roots when plant is dormant-ideally early spring (4) Seed per Pound 3600000 Seed Spread Rate Moderate (3) Place end of branch with female catkins in water of 8-10 C, high air temperature, and low relative humidity. Remove seeds from capsule once it opens. (4) Plant cuttings 1.3cm deep in vermiculite for six weeks. Cut suckers and place in vermiculite: perlite mixture and mist for 2-3 weeks. Transplant to peat: vermiculite mixture for growth. Temperatures between 15-25 C. (4) Length of Storage Dry seeds for three days at 24 C. Viable for one year stored at 5 C and 5-8% moisture content. (4) Guidelines for Outplanting / Performance on Typical Sites References Recommended planting density-close proximity, within 25' (5) Planting Density per Acre, Minimum 300 Planting Density per Acre, Maximum 1200 (3) INFORMATION SOURCES 1. Lyons. C.P, Bill Merilees. Trees, Shrubs & Flowers to Know in Washington & British Columbia. Lone Pine Publishing. 1995. Retrieved April 9, 2008 2. Pojar, J. and MacKinnon, A. 1994. Plants of the Pacific rthwest Coast. Lone Pine Publishing, Redmond, WA.

3. USDA Natural Resource Conservation Service. Plants Profile. http://plants.usda.gov/java/namesearch?keywordquery=mahonia+nervosa&mode =sciname. Retrieved: April?, 2008. Other Sources Consulted Protocol Author Date Protocol Created or Updated 4. Rose, Robin, Caryn Chachulski, and Diane Haase. Propagation of Pacific rhtwest Native Plants. Oregon State University Press, Corvallis, OR. 1998. 5. Rook, Earl. Plants of the rth. http://www.rook.org/earl/bwca/nature/flora.html. September 27, 2002. 1. Eliot, Willard. Forest Trees of the Pacific coast. G.P Putnam s sons. 1938. Retrieved April 9, 2008. Kevin Wilen 04/29/08 Plant Data Sheet Species (common name, Latin name) Quaking Aspen, Populus tremuloides Range Most widely distributed tree in rth America, throughout most of the continent from Newfoundland to Alaska, down to the mountains of northern Mexico (Rook, 2002) Climate, elevation Colder inland climates over humid coastal climates; near sea level to 3050m (Rose et al., 1998) Local occurrence (where, how common) Widely used in landscaping for noise reduction and visual screening (Rook, 2002); along waterways, road cuts, and forest edges.

Habitat preferences Well drained, high in calcium, loamy soils. Sites disturbed by fire and logging with exposed mineral soil. Grows along forest edges and waterways-shade intolerant (Rose et al., 1998) Plant strategy type/successional stage (stress-tolerator, competitor, weedy/colonizer, seral, late successional) Occurs in pure stands as a succesional species that is replaced by conifers (Rook, 2002) Associated species Acer rubrum, Abies balsamea, Fraxinus nigra, Populus spp., Cornus spp., Rubus spp., Aster macrophyllum, Smilacina stellata, Galium triflorum (Rook, 2002) May be collected as: (seed, layered, divisions, etc.) Seed and vegetative root suckers (Rose et al., 1998) Collection restrictions or guidelines Collect seed from May to mid-june. Collect cottony, wind-borne seeds about a week before capsule opens. Collect lateral roots when plant is dormant-ideally early spring (Rose et al., 1998) Seed germination (needs dormancy breaking?) Place end of branch with female catkins in water of 8-10 C, high air temperature, and low relative humidity. Remove seeds from capsule once it opens. (Rose et al., 1998)

Recommended seed storage conditions Dry seeds for three days at 24 C. Viable for one year stored at 5 C and 5-8% moisture content (Rose et al., 1998) Propagation recommendations (plant seeds, vegetative parts, cuttings, etc.) Optimal germination of seeds is at 15-25 C. Surface sow in very moist seedbed; keep well watered. Collect roots for vegetative reproduction that are 1-2cm in diameter and 2.5cm in length (Rose et al., 1998) Soil or medium requirements (inoculum necessary?) Plant cuttings 1.3cm deep in vermiculite for six weeks. Cut suckers and place in vermiculite:perlite mixture and mist for 2-3 weeks. Transplant to peat:vermiculite mixture for growth. Temperatures between 15-25 C (Rose et al., 1998) Installation form (form, potential for successful outcomes, cost) Container, 12-48 (Fourth, 2003); 0.5-1 gallon (Watson, 2003) Recommended planting density Close proximity, within 25' (Rook, 2002) Care requirements after installed (water weekly, water once etc.) Must be planted in soil with high moisture content or watered on a daily basis (Watson, 2003) rmal rate of growth or spread; lifespan Rapid growth rate, 40ft. at 20 years of age, 65ft. at maturity (VegSpec, 2003); 100+ years old for vigorous individuals (Perala, 1990) Sources cited 1) Fourth Corner Nurseries. www.4 th -corner-nurseries.com; April 7, 2003 2) Perala, D.A. Silvics of rth America. http://www.na.fs.fed.us/spfo/pubs/silvics_manual/volume_2/populus/tremuloides.htm. USDA, Forest Service, Agriculture Handbook 654.December 1990. 3) Rook, Earl. Plants of the rth. http://www.rook.org/earl/bwca/nature/flora.html. September 27, 2002. 4) Rose, Robin, Caryn Chachulski, and Diane Haase. Propagation of Pacific rhtwest Native Plants. Oregon State University Press, Corvallis, OR. 1998. 5) VegSpec. Phil Smith, Project Manager. http://ironwood.itc.nrcs.usda.gov/netdynamics/vegspec/pages/homevegspec.htm, USDA, Natural Resource Conservation Service. April 7, 2003.

6) Watson, Rae. Forestry Technician. http://nativeplants.for.uidaho.edu/network/view.asp?protocol_id=2370. USDA, J. Herbert Stone Nursery. Protocol Information. Data compiled by (student name and date) Scott Olmsted; 041403