Operator s Manual. The Quietek

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Operator s Manual The Quietek Congratulations! Congratulations on your purchase of the Quietek euthanization system. Please read this operator s manual which explains proper operation of the instrument. This manual is posted on our website, www.nextadvance.com. Click the SUPPORT button on the lefthand menu bar and then on the appropriate link to the manual. We re confident that your Quietek will become an essential tool in your facility and we wish you success with your work. Contents Parts of the Quietek...2 Setup...3 Operation...3 Protocols and Sample Settings...4 Cleaning...5 Troubleshooting...5 Support...5 Specifications...5 Warranty...6 Warnings and Cautions...6 Disclaimer...7 Contact Info...8

Quietek Components 2

Quietek Components Caution: Follow the Instructions in the Operator s Manual This product complies with European Low Voltage and EMC Directives 3

Preparing the Quietek for Use Insert the power supply (round) plug into the receptacle OR power jack on the back of the Quietek controller, and plug the power supply into a standard outlet. Connect the delivery hose to its fitting on the controller, and attach the other end to the barbed fitting on the cage lid. The Quietek 2 has enough fittings to operate with up to two cages. Ensure that the lid is large enough so that bent edges fit entirely around the rim of the cage. If any part of the bent metal edge of the lid is in contact with the cage rim, the lid may not seat properly. Adjusting the Settings Before you begin: Determine the volume of your cage, in liters. A cage volume calculator is available at cagevol.nextadvance.com. Select the setting that you wish to adjust (A or B) using the Cage Size switch. The Quietek can store two different cage volume/flow rate combinations. Turn the key to the "Adjust" position. Set the cage volume using the Cage Vol. knob. Measurements are shown in liters. Set the desired fill rate using the Fill Rate knob. Flow rate is controllable within the range specified in the AVMA guidelines. If you have a Quietek XL model, you will not be able to use very low flow rates at small cage sizes (below 60L). Turn the key to the "Run Mode" position. If the key is removed in run mode, the machine will still run and the settings will not be adjustable. Note: For the Quietek 2, settings A or B depending on the orientation of the Cage Size switch) will apply to every cage or chamber that is connected to the device. Performing a Run For every cage or chamber being used, confirm that the lid is properly fitted, with the lining in full contact with the cage rim. Open the valve on the CO2 source. Ensure that the key lock is in the "Run Mode" position. Run will not start if the key is in the "Adjust" position. Press the "Start/Stop" button. If you are using the Quietek 2, press the Start/Stop button that corresponds to the cage(s) you want to use. The "CO2 fill" light corresponding to the cage you are using will illuminate, and the timer will count down until one cage volume of gas has been delivered. When the cage is filled, the CO2 flow will stop. The "Immersion" light will illuminate and the timer will show time elapsed since immersion began. To stop the run, press "Start/Stop" again. The controller will reset, and another run can be performed with the 4

same settings if desired. The CO2 supply does not need to be shut off between runs. If you wish to perform a run with different settings, you can change the Cage Size switch to the other position if the settings have been saved previously. To perform a run with new settings, adjust the parameters as described in the "Adjusting the Settings" section above. Emergency stop: The run can be stopped at any time by pressing the "Start/Stop" button. Note: If the CO2 pressure is below 15 psi, the digital display will alternately flash CO2 and LO. Purging the Chamber To purge, tip the chamber over to release CO2. This process may take several minutes based on the size of the chamber and air flow in the area. Cleanup After use, shut off the CO2 supply. Wipe down the inside of the lid with warm water and then spray with 70% ethanol or another suitable cleaner. The unit may be unplugged if desired. The Quietek controller box can also be wiped down with a water and mild detergent such as Alconox. Ethanol or other alcohols will dull the finish of the box. The unit should not be immersed in liquid. Troubleshooting If the Quietek doesn t turn on, the plug of the power supply cord may not be in a live wall outlet or the power supply connector may not be fully inserted in the socket on the side of the Quietek. If the cage volume indicator is flashing CO2 and LO, the CO2 pressure from the supply tank or line is too low for the Quietek to operate properly. Check the CO2 source to make sure that it is securely connected to the device. Some institutions may require external confirmation of the gas flow rate from the Quietek controller. This may be most conveniently done by disconnecting the lid and attaching a precision CO2 flowmeter to the tube. Support FAQs and other helpful information are available on our website, http://www.nextadvance.com. Click on the Products button on the left-hand menu bar and then on the link to the Quietek. If you cannot find an answer there, please contact customer service by email at support@nextadvance.com or by telephone at 1.518.674.3510. 5

Specifications Size: depends upon model Weight: depends upon model Power Requirement: 12 V, 0.70 Amp Relative Humidity: 5-90% noncondensing Operating Temperature: 4-40 C Altitude: <2000m Storage Temperature: -40-40 C CO2 Pressure Requirement: 20-75 psi Meets requirements (-CE models only) Warranty Next Advance warrants its Products against defects in materials and workmanship for time periods which vary according to the Product. Within these time periods, Next Advance will replace or repair, without charge to the original purchaser, any part which is defective. Quietek warranty = One year The warranty is void if the Product is defective due to product accident, product modification, exposure to radiation other than for sterilization, connection to an improper electrical supply, lack of proper maintenance, contamination, improper installation or misuse. If the product is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. The warranty shall also not apply to defects arising from fire, flood, lightning or other conditions unrelated to correct operation of the Product. Next Advance s liability is limited, at the company s election, to (1) refund of the original purchaser s purchase price for the Product (2) repair of the Product, or (3) replacement of the Product or defective parts. Evidence of purchase by the original purchaser is required. Next Advance may also request documentation of proper maintenance, if applicable. Next Advance makes no other warranty, expressed or implied, with respect to its Products. NEXT ADVANCE MAKES NO WARRANTY RESPECTING THE MERCHANTABILITY OF THE PRODUCTS OR THEIR SUITABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE OR USE. Next Advance shall not be liable for indirect, special, incidental or consequential damages of any nature. Any recovery for any claim shall be limited to the original purchase price for the product. OPERATOR S RESPONSIBILITY Provide proof of purchase and provide normal care and maintenance. Warnings and Cautions Read the use s manual before operating. No serviceable parts are inside. Use only with CO2 supply line or tank. For indoor use only. Pollution Degree 2 per EN 61010-1. 6

Enclosure Protection: Not Protected Against the Ingress of Moisture. Do not immerse in liquid. Before using the Quietek, touch a bare metal surface to discharge static electricity. Disclaimer Next Advance, Inc. makes no representations or warranties, expressed, statutory or implied, regarding the fitness or merchantability of this product for any particular purpose. In no event shall Next Advance be liable for incidental or consequential damages. Next Advance, Inc. is not liable for any damages, including but not limited to, lost profits, modifications to the product described in lost savings, or other incidental or consequential damages arising from ownership or use of this product, or for any delay in the performance of its obligations under the warranty due to causes beyond its control. Next Advance, Inc. also reserves the right to make any improvements or changes to this manual at any time, without notice of these changes. Next Advance, Inc. products are not designed, intended, or authorized for use in applications or as system components intended to support or sustain human life, as a clinical medical device for humans, or for any application in which the failure of the product could create a situation where personal injury or death may occur. All brand and product names used in this manual are the trademarks of their respective owners. Note: No animals were harmed or euthanized for the main purpose of developing or testing this product. Contact Information Next Advance, Inc. Averill Park, NY, USA Telephone 518-674-3510 x3 www.nextadvance.com Email: support@nextadvance.com 7