WTAMU Academic Research Environmental Health and Safety (AR-EHS) Laboratory Inspection Checklist

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WTAMU Academic Research Environmental Health and Safety (AR-EHS) Laboratory Inspection Checklist Date of Inspection: Laboratory Title: Location (room # & building): Principal Investigator/Supervisor: Site/Lab Safety Coordinator/Lab Contact: Conducted By: Chandini Revanna Office number: Cell number: 1 I. Laboratory Work Practices No food & beverages rules are observed. Food and beverages are not stored in the laboratory areas, refrigerators or in glassware that is also used for laboratory operations. Pipetting is performed by mechanical means. Laboratory surfaces are cleaned; disinfected or decontaminated after work is performed. Required PPE is being worn. Used needles are stored in appropriate sharps containers Syringes are needle locking. No recapping of needles is performed. Hoods are not being used for storage. Hood sash is closed when not in use Yes No COS (Corre cted on site) N/A Comments II. Housekeeping Laboratory and storage areas uncluttered and orderly (including bench top). Aisles & exits are free from obstruction. Work surfaces are protected from contamination. Electrical cords are in good condition and are UL listed.

Tools and equipment are in good repair and electrically grounded. Tops of cabinets and shelves are free from stored items. Heavy objects are confined to lower shelves. Glassware is free from cracks, chips, sharp edges and other defects. Sharps containers are available and in use Broken glass containers are available and in use. Soap and paper towel are present. 2 III. Personal Protective Equipment Protective gloves are available and matched to hazards involved. Eye protection is available and in use in the laboratory. Lab coats, tyvek garments etc. are available and in use. Lab coats are only worn in the laboratory and are removed before entering offices, lunchrooms, rest rooms, conference rooms and other non-laboratory general use areas. (This includes disposable protective clothing). Dirty lab coats/uniforms are stored in a covered container until removed for laundering. Appropriate protective clothing is available and in use when working with radioactive materials. Respirators are provided when necessary, and selected on the basis of hazard present. Respirators are used correctly, cleaned after every use and stored in a convenient, clean and sanitary area. Contact AREHS to access laundry facility. IV. Hazard Communication Primary & secondary chemical containers are labeled with identity, appropriate hazard warnings, and expiration dates. Signs on storage areas (e.g. Refrigerators) and laboratories are consistent with hazards within.

MSDS binders are available for chemicals used and stored in area. Employees know the location of the MSDS binders for their work area. Satellite MSDS collections are complete and readily available at all times to laboratories. MSDS Material Safety Data Sheet is now called SDS Safety Data Sheet. 3 V. Chemical Storage Incompatible materials are segregated. Corrosives and flammables are stored below eye level. Hazardous materials used/stored in the laboratory are limited to small quantities. Unnecessary, unused, or outdated materials are removed from laboratories and chemical storage areas. Glass chemical containers are not stored on the floor Chemicals stored in acceptable container Safety carriers are available and in use while transporting chemicals. All lab carts have side-rails. All containers are properly labeled with: Name, Date, Contents, Lab # Label peroxide formers and time sensitive chemicals like chloroform with received date and opened date Dispose of peroxide forming chemicals that have been stored for over one year or open for more than 6 months unless regular peroxide testing confirms the absence of peroxide formation Acids are stored in acid cabinet or plastic secondary containment VI. Flammable Liquids Storage & Handling Flammable liquids are stored and used away from ignition sources.

Bulk quantities of flammable liquids are stored in approved storage cabinets. Flammable liquid storage cabinets are properly labeled. Flammable liquid storage cabinets close properly. Flammables stored on open shelves in glass or plastic containers are within permissible quantities (limit = < 10 gallons) Safety cans used to handle small quantities of flammable liquids are properly labeled. Solvent waste cans are labeled properly with: Name, Contents, Lab #. Nothing is stored on top of flammable cabinets. Flammables are not stored in household refrigerator 4 VII. Compressed Gas Cylinders Gas cylinders are properly chained/secured. Cylinder caps are in place when cylinders are not in use or being moved. Gas cylinders are transported on a cart with chains. Gas cylinders are stored away from excessive heat. Fuel gas cylinders are at least 20 feet away from oxygen cylinders. Gas cylinders are properly marked as to their contents. Full and empty cylinders are stored separately. Empty gas cylinders are labeled EMPTY. Gas lines, piping, manifold, etc. are labeled with the identity of their contents. Hoses, tubing and regulators are in good working condition. Gas cylinder dating is observed (* indicates can be stored for 10 yrs) Highly flammable, toxic or corrosive gases are properly ventilated

5 VIII. Waste Handling: Hazardous, Non-Hazardous & Biological Liquid waste disposed of in the sinks meets drain disposal requirements in SOP 24.01.01.W1.06AR. Hazardous wastes are not accumulated for longer periods. Waste streams are separated as necessary: ex. Solid vs. liquid, hazardous vs. non-hazardous, halogenated vs nonhalogenated, etc... Waste containers are appropriately tagged before placing in waste room. Containers of hazardous waste are labeled properly with the Chemical names of all the contents date and name of person discarding waste. Biological waste is appropriately marked with a biohazard symbol. Biohazard waste is kept in secondary container. Biohazard waste is autoclaved Autoclave log is maintained in the autoclave room Autoclave is on periodic efficiency monitoring schedule (TCEQ) Syringes and other sharp waste are disposed of into a sharps container and placed directly into biohazard waste container. Waste material is not allowed to accumulate on the floors, in corners or under shelves/tables in laboratories. Radioactive waste is properly marked with radiation symbol. All waste containers are closed unless actively pouring chemicals. IX. Means of Egress and Emergency Exits Exits are clearly marked. Exits are free from obstruction. All fire doors are self-closing. All fire doors are kept closed.

Fire alarms are provided. Emergency numbers posted on or near laboratory door. Emergency evacuation routes are clearly posted. Emergency evacuation routes are posted in common hallways. Emergency exit lights are working and clear of obstruction. 6 X. Safety Equipment Safety shower present? If yes, date of inspection Eyewash station present? If yes, weekly activation log issued Safety showers and eye wash stations are clearly labeled, and these areas are clear from obstruction. All showers and eye wash stations are clean, covers are replaced and in good working condition. After activating safety shower install tag indicating date of activation, apr flow rate Fire extinguishers are located within 50 of the laboratory and wall mounted. Fire extinguishers are the appropriate type for the hazard in the work area. Fire extinguishers are present. Date of last inspection: Fire detection devices, smoke alarms, sprinkler systems, lighted exit signs are in good working condition. First-aid supplies are readily available and clearly visible. Chemical spill team list is clearly posted in laboratories. Chemical spill kit is complete and available. Fume hood present? If yes, date of inspection. Biosafety cabinet present? If yes, date of certification ------------- AREHS to implement spill response team and update the contact information on the spill kit.

Blood borne pathogen spill kit is complete and available. 7 XI. Labeling, Posting and Training Warning signs and labels are present whenever required (e.g. carcinogen, mutagen) where chemicals are stored. Caution - Radioactive Material signs are posted on doors of all authorized laboratories and on refrigerators/freezers where materials are stored. Biohazard symbols are posted on access doors to biohazard laboratories and animal rooms and on potentially contaminated equipment. All Faculty, Staff, and Student Lab Training completed Is Faculty, Staff, and Student Bloodborne Pathogen (BBP) Training required? Is the Chemical Hygiene Plan (CHP) located at the front of the MSDS binder? MSDS sheets for each chemical used in the laboratory are updated and posted in MSDS Binder Chemical inventory present NFPA door placards are current and posted NFPA-704 and Safety Data Sheets Hazard Identification PI to ensure that all lab personnel have undergone necessary safety trainings. MSDS Material Safety Data Sheet is now called SDS Safety Data Sheet. AREHS has successfully implemented chemical inventory though barcode system (currently-inprogress). AREHS to update NFPA door placards, after updating the inventory. In transition phase to GHS as best practice. Manufacturers are required to provide Safety Data Sheets. Contact manufacturer to ensure that you will receive the SDS on all orders. When reviewing the latest Safety Data Sheets, ensure to understand the difference in hazard ratings between NFPA-704 and Safety Data Sheets GHS Global Harmonization System. http://www.nfpa.org/newsandpublications/nfpa-journal/2013/mayjune-2013/features/working-together https://www.osha.gov/dsg/hazcom/ghs.html

8 XII. Fire, Electrical, Guards, Rails Power strips and extension cords are not connected in series. Extension cords are not used as permanent wiring. Electrical cords, thermostats are not in poor condition Vacuum pumps, paddle drags etc have guards for safety Ceiling clearance 24 (without sprinklers) and 18 with sprinkler system Sprinkler heads are not blocked XIII. Miscellaneous Lab specific/site specific risk assessment, responsibilities, training and documentation discussed with PI/Supervisor/Safety Coordinator. All lab personnel have undergone all necessary safety training requirements PI to ensure that all lab personnel have undergone necessary safety trainings. List of Research funding agencies if any - Any specific type of Research Ensure to implement and follow all funding agency safety requirements put forth in the research Grant/protocol/contract. Any international activity Total number of authorized users in the lab/site - Previous incident/injury/illness/near miss/any other concerns/data Date of the incident and status of the lab personnel employee/student

First report of injury/student Health Services notified 9 There is no chemicals/biological/radioactive substances shipped locally or internationally out of WTAMU from this lab/site. There is no dry ice shipment going out of this lab/site. Has completed IRB/IBC/IACUC application process. Lab/Site HVAC system has no complaints. All chemical/biological/radiological supplies are ordered through AREHS. No slip trip and fall hazard noted. Previous Inspection Action Items Miscellaneous XIV. Follow up Recommendations/Notes:.... XV. Action Items: Responsible Party: 1. PI to ensure all lab personnel have undergone necessary safety trainings including site specific training and documentation. Due Date 8/10/2013 Contact: aswindell@wtamu.edu Please email April Swindell, upon completion of action items. Follow up date: 8/12/2013-8/19/2013 Responsible Party AREHS: 1. AREHS to issue lab specific training documentation guidance/checklist.

2. PI and AREHS to ensure that all lab personnel have undergone necessary trainings. 10 Inspections/Walk-through completed by: Chandini Revanna Principal Investigator/Supervisor: Office Phone: 2740 Chandini Revanna, CIH Director, AR-EHS Dr. Angela Spaulding, PhD Research Compliance Officer Questions? Please contact the AR-EHS office at 651-2270 or email April Swindell at aswindell@wtamu.edu Note: WTAMU AR-EHS Inspection checklist liberally drafted from Laboratory Inspection Checklist (International R&D).