Fungal Phytopathogen Evaluation Report Type: Spore trap Client information Name: Dave Bell Address: 244 Jamieson Rd, Harvey Station Sample information Lab ID : NB-220818AB Nature : Spore trap Methodology : M-AC-12-10 Air volume : 225L Sampling date : 08-22-2018 Received date : 08-22-2018 Analysis date : 08-22-2018 Report date: 08-23-2018 Sample condition when received : Satisfactory Limit of detection : 4 tfp/ m3 Page 1
Proliferation index Sample(s) Debris density EDL-1 3 CT-1 2 RD-1 2 SP-1 2 HF-1 2 Molds and/or bacterias Quantity (spores/m3) Identification of causal agent Associated disease Previous Actual Previous Actual Alternaria solani/alternata Early blight 36 13 18 Botrytis cinerea Grey mould 9 9 67 Alternaria solani/alternata Early blight 27 31 4 Botrytis cinerea Grey mould 524 89 27 Alternaria solani/alternata Early blight 9 31 44 Botrytis cinerea Grey mould 27 36 40 Alternaria solani/alternata Early blight 9 31 4 Botrytis cinerea Grey mould 9 31 284 Alternaria solani/alternata Early blight 44 13 58 Botrytis cinerea Grey mould 200 40 36 Risk Page 2
HF-2 2 EA-1 2 KF-1 2 RC-1 2 BY-1 2 Alternaria solani/alternata Early blight NA NA 4 Botrytis cinerea Grey mould 58 80 480 Alternaria solani/alternata Early blight NA NA NA Botrytis cinerea Grey mould 124 NA 4 Alternaria solani/alternata Early blight NA 4 NA Botrytis cinerea Grey mould 31 53 58 Alternaria solani/alternata Early blight NA 9 NA Botrytis cinerea Grey mould NA 338 707 Alternaria solani/alternata Early blight NA NA NA Botrytis cinerea Grey mould 27 98 102 We observed 22 spores/m3 of other Oomycetes in SP-1 sample. Other Oomycetes are late blight marker Weather-based risk assessment scale for the proliferation of each type of pathogen. Page 3
In this report, you will find two different tools to make the right decision; the spore counts and the proliferation risk assessment based on current and past meteorological parameters. Laure Cockenpot, Microbiologiste M.Sc. Note : The results exposed in this report are only linked to the samples and method described above. Reproduction of this report, in part or in its entirety is forbidden without written consent and approval from the authorized signatory. Interpretation guide The debris scale is divided as follow : 0 : No debris. 1-2 : Low proportion of debris -> No (or weak) interference. 3-4 : Important proportion of debris -> Possible interference. Interpret with caution. 5 : Debris proportion too high -> Analysis is impossible. Inadequate sample. Page 4
Definitions PHYTOPHTHORA INFESTANS: Pathogen belonging to the Oomycetes family, Phytophthora infestans is the causal agent of the late blight of potato. It is responsible for massive destruction of crops, including a famine in Scotland and Ireland. This organism is usually found in potato crops and tomatoes. The first signs of infection may appear as early as May, usually in the more humid areas. This pathogen requires a relative humidity percentage higher than 90% and a cool temperature (16 C. At 20 C day and 10-15 C at night). The spores are very sensitive to UV rays. The time between the deposition of the spore on the plant and the formation of lesions is usually between 7 to 10 days. ALTERNARIA SOLANI/ALTERNATA: Early blight appears after flowering and is caused by molds of the genus Alternaria. The disease is recognized by the appearance of brown leaf spots with concentric rings. The disease development is rather slow and much less damaging than late blight but can still cause early senescence and a significant loss of performance. The spores are very resistant to UV rays and the disease develops more easily following a deficiency or drought, with high humidity and warm temperatures. FUSARIUM SPP.: Several species of Fusarium can attack potato plants and cause fusarium dry rot. The problems associated with this pathogen occur both in the field warehouse. The risk of developing the disease increases by growing potatoes in successive years in the same field and when temperatures are warm combined with frequent rainfall. BOTRYTIS CINEREA: Pathogen able to infect multiple crop types. Commonly called botrytis, it may infect fruits, leaves and flowers. It s recognizable by its gray mycelium that appears following extended moisture periods. It grows mainly in locations where the plant is already infected. Page 5
Annex Fungal Phytopathogen Associated disease 13-06-2018 14-06-2018 17-06-2018 20-06-2018 23-06-2018 24-06-2018 NA NA NA Alternaria solani/alternata Early blight 4 NA 8 17 NA 4 NA NA NA Botrytis cinerea Grey mould 30 83 106 65 13 26 Fungal Phytopathogen Associated disease 27-06-2018 28-06-2018 30-06-2018 03-07-2018 05-07-2018 08-07-2018 NA NA NA Alternaria solani/alternata Early blight 8 25 8 56 83 NA NA NA NA Botrytis cinerea Grey mould 48 8 34 315 25 21 Fungal Phytopathogen Associated disease 10-07-2018 12-07-2018 13-07-2018 16-07-2018 19-07-2018 21-07-2018 NA NA NA Alternaria solani/alternata Early blight 30 30 4 9 138 97 NA NA NA Botrytis cinerea Grey mould 43 155 66 48 280 123 Page 6
Fungal Phytopathogen Associated disease 24-07-2018 26-07-2018 28-07-2018 31-07-2018 02-08-2018 04-08-2018 NA NA NA Alternaria solani/alternata Early blight 4 97 390 133 22 31 NA NA NA Botrytis cinerea Grey mould 73 172 141 13 27 18 Fungal Phytopathogen Associated disease 07-08-2018 09-08-2018 11-08-2018 14-08-2018 16-08-2018 18-08-2018 NA NA NA Alternaria solani/alternata Early blight 86 13 173 36 76 36 NA NA NA Botrytis cinerea Grey mould 173 49 36 13 227 9 Fungal Phytopathogen Associated disease 21-08-2018 Phytophthora infestans Late blight NA Alternaria solani/alternata Early blight 13 Fusarium spp. stem rot NA Botrytis cinerea Grey mould 9 Page 7