Butac Madalina, Botu Mihai, Militaru Madalina, Mazilu Craisor, Dutu Ion, Nicolae Silvia

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PLUM GERMPLASM RESOURCES AND BREEDING IN ROMANIA Butac Madalina, Botu Mihai, Militaru Madalina, Mazilu Craisor, Dutu Ion, Nicolae Silvia

PLUM CULTURE IN EUROPE AND ROMANIA (FAO data, 2018) Surface (ha) Crop (to) (to/ha) Europa Serbia Romania 391 469 77 949 65 114 2 636 221 463 115 512 975 6.7 5.9 7.9 10.8 % 17.5 %

I. INTRODUCTION Romania is a country located in South East Europe which has good environmental conditions for many fruit species in the wild or cultivated status. Numerous genetic resources of plum, apple, pear, sweet and sour cherry, peach, apricot, walnut, hazelnut, sweet chestnut, berries are present (Botu et. al., 2017). In Romania, after 1970, identification, conservation and evaluation of fruit genetic resources activities were started in order to limit the loss of the biodiversity due to erosion and genetic vulnerability. The genetic resources preserved by ex situ and in situ methods are very important value and can be use for breeding new cultivars and rootstocks. The success of any breeding program depends on the existence of a rich and valuable germplasm fund.

I. INTRODUCTION Presently, in Romania there are plum collections in two centers: RIFG Pitesti and UCv-SCDP Vâlcea. I. Botu, M. Botu, E. Turcu S.C.D.P. Vâlcea 1950 V. Cociu, R. Roman, M. Butac I.C.D.P. Piteşti

SITUATION OF EX SITU PLUM COLLECTIONS No. Center Type of collections Species and intersp. hybrids Local accessions Foreign accessions Other (biotypes, hybrids, mutants) Total no. of accessions 1 RIFG Pitesti Cultivars 7 183 320 40 550 Rootstocks 0 82 10 0 92 TOTAL RIFG 7 265 330 40 642 2 UCv-SCDP Vâlcea TOTAL ECv- SCDP TOTAL GENERAL Cultivars 27 56 125 35 216 Rootstocks 86 21 11 118 27 142 146 46 361 34 407 476 86 1,003

In these collections of cultivars and rootstocks, have been made observations and determinations regarding: - phenology, - productivity, - vigor, - fruit quality, - resistance/tolerance to diseases and pests, - resistance to low temperatures, - resistance to frost. The observations and measurements were done according to the IBPGR Prunus descriptors updated by the ECP/GR Prunus Working Group members within the Genres CT95 No 61 project in titled International network on Prunus genetic resources. in order to select genitors for breeding works.

THE MAIN OBJECTIVES IN PLUM BREEDING IN ROMANIA CULTIVARS 1960 1980 - Improvement of old cvs. Tuleu Gras Vinete Romanesti Grase Romanesti - Selection inside of wild population ROOTSTOCKS 1980 2000 - Fruit quality for fresh market - Ripening season extension - Tolerance to PPV 2000 present - Tolerance to PPV -Fruit quality -Yielding capacity - Self fertility low to medium induced vigour tolerance to PPV and foliar diseases easy prpagation adaptability to heavy soil good anchorage in the soil

GENITORS USED IN PLUM cvs. BREEDING BASIC GENITORS Tuleu gras Centenar Carpatin Tuleu timpuriu Tita Alina Record Minerva Pitestean Valcean Stanely Renclod Althan Anna Spath CHARACTER GENITORS TOLERANCE TO PPV FRUIT QUALITY YIELDING CAPACITY SELF FERTILITY EARLINESS Oneida Kirke Grase de Becs Jojo Grande Prize Oneida Vision Romanta Valcean Stanley Anna Spath Standard Cacanska lepotica Romanta Stanley Anna Spath Cacanska lepotica Ialomita Early Rivers Ruth Gerstetter Ialomita Diana

GENITORS USED IN PLUM ROORSTOCK BREEDING Prunus domestica Prunus cerasifera Prunus insititia Rosior varatec Rencold verde Brompton Otesani 8 Voinesti B Corcodus 163 Corcodus 169 BN4Kr Myrobalan selections Pixy Otesani 11 Saint Julien A Scoldus Albe mici Porumbar de Iasi Porumbar Brooks P. besseyi selections Orient Prunus spinosa Prunus besseyi Prunus tomentosa

BREEDING METHOD AND RESULTS FOR CVS. Controlled hybridization Open pollination Selection Mutagenesis RIFG PITESTI 23 CULTIVARS 1,750 hybrid combinations 2,000,000 pollinated flowers 300,000 hybrid stones 125,000 hybrids seedlings 350 promising selections 40 CULTIVARS RSFG VÂLCEA 6CULTIVARS RSFG BISTRITA 11 CULTIVARS

BREEDING METHOD AND RESULTS FOR ROOTSTOCKS Selections from wild and cultivated native flora (nursery seed beds included) Inter and intraspecific crosses RIFG PITESTI 4 rootstocks: 2 generative 2 vegetative RSFG BISTRITA 1 generative rootstocks RSFG VALCEA 7 rootstocks: 1 generative 6 vegetative

EVOLUTION OF PLUM ASSORTMENT Grase romanesti Gras ameliorat Vinete românesti Vinete româneşti 300 1960-1980 Tuleu gras Tuleu timpuriu Superb Tuleu dulce Albatros Centenar Pitestean Dambovita Carpatin Minerva Flora Sarmatic Baragan 17 Tita Alina Iulia Ivan Jubileu 50 Roman Romaner Dani Geta Elena Topval 1960 - prezent Other Silvia Pescarus Ialomita Diana Record Valcean Renclod de Caransebes Agent Andreea Delia Doina Matilda Zamfira Alutus Romanţa

RIPENING SEASON

CENTENAR (Tuleu gras x Early Rivers) Earliness Good yielding capacity Excelent taste PITESTEAN (Tuleu timpuriu x Early Rivers) Earliness Large fruit(50 g) Good yielding capacity

TITA (Tuleu gras irradiated stones) Earliness. Good yielding capacity Good quality fruit CARPATIN (Tuleu gras x Early Rivers) Earliness. Good yielding capacity Large fruit (50 g) Tolerance to PPV.

PESCARUS (R.C. Althan x Wilhelmina Spath) High productivity. ALBATROS (Tuleu gras Open pollination) Excellent taste

AGENT (Selection in wild population) High content in sugar (over 25% soluble solids content) Tolerance to PPV. ROMAN (Tuleu gras x Early Rivers) Large fruit (> 45 g) Tolerance to PPV.

ROMANŢA Stanley x Vâlcean High productivity Large fruit (60 g) Tolerance to PPV Selfertility

ADAPTABIL Named in 2000 year; Origin: Prunus besseyi x mixed pollen from Prunus sp.; probably a P. besseyi x P.persica hybrid; Vegetative rootstock for peach and nectarine cultivars and also for plum; Tolerant to foliar diseases and to Plum-pox virus; Very easy to propagate by softwood cuttings (over 90 % rooted cuttings); Medium to low influence for vigor in orchard, and long life for the grafted trees (18-20 years).

MIRODAD 1 Mirobolan dwarf x Adaptabil Vegetative rootstock for high density European PLUM orchards.

CORVAL Selection in P. cerasifera Population Generative rootstock for high density orchards

THANK YOU FOR ATTENTION!