20130111a.pdf IR-4 Ornamental Horticulture Program Research Report Cover Sheet Researcher(s) Gary Chastagner Affiliation: Washington State University Trial: 2012 WA Pullayup WA PhoneNumber: (253) 445-4528 Email: chastag@wsu.edu ProjectTitle: Botrytis Efficacy Protocol #: 12-017 PR# Research Target Crop/Plant Product EPA Reg. # Production Site Status 30556 Botrytis elliptica Lily Chipco 26019 N/G 432-889 Field In-Ground C Botrytis elliptica Lilium sp. Iprodione Bayer 30448 Botrytis elliptica Lily Disarm 480SC 66330-64 Field In-Ground C Botrytis elliptica Lilium sp. Fluoxastrobin OHP 30480 Botrytis elliptica Lily Pageant 38WG 7969-251 Field In-Ground C Botrytis elliptica Lilium sp. Boscalid + Pyraclostrobin BASF 30554 Botrytis elliptica Botrytis elliptica Lily Lilium sp. Palladium Cyprodinil + fludioxanil 100-1328 Syngenta Field In-Ground C 31356 Botrytis elliptica Lily Proud 3 - Field In-Ground C Botrytis elliptica Lilium sp. Thyme oil (5.6%) Biohumanetics 31360 Botrytis elliptica Botrytis elliptica Lily Lilium sp. Regalia 5O (MOI-10605) Extract of Reynoutria sachali - Marrone Bio In Field In-Ground C 31357 Botrytis elliptica Lily Torque 3.6SC 1001-87 Field In-Ground C Botrytis elliptica Lilium sp. Tebuconazole Cleary 31358 Botrytis elliptica Lily Tourney 50WDG 59639-144 Field In-Ground C Botrytis elliptica Lilium sp. Metconazole Valent 30449 Botrytis elliptica Botrytis elliptica Lily Lilium sp. Trinity 2SC Triticonazole - BASF Field In-Ground C 31359 Botrytis elliptica Lily V-10135 - Field In-Ground C Botrytis elliptica Lilium sp. Fenpyrazamine Valent 31361 Botrytis elliptica Botrytis elliptica Lily Lilium sp. ZeroTol Hydrogen dioxide - Field In-Ground C
Ornamental Horticulture Program Research Report Form p 1 Researcher: Gary Chastagner and Annie DeBauw Date: 12/5/2012 Project Title: L-112; IR-4 Efficacy of Management Tools for Botrytis on London Lilies Protocol #: 12-017 PRnumbers: 30556, 30448, 30480, 30554, 31356, 31360, 31357, 31358, 30449, 31359, 31361, Narrative Summary (Results/Discussion) Please keep text to one page if possible. Include summary of trial results and a brief discussion including how any changes from the protocol may have affected results. Results for multiple PRnumbers can be summarized together, but please list all PRNumbers in the header and in the summary data table. Disease pressure in this field trial was low. Initial treatment applications on the lilies started June 11, 2012 and ended September 7, 2012. Disease incidence was rated on a scale of 0 10, where 0 = none, 1 = 1-10%, 2 = 11-20%, and 10 = 91-100% of the plants were diseased. By September 19, 2012 (day 100), the average disease incidence rating ranged from 2.3 to 9.5. The ANOVA analysis of data in this test indicated that there was a highly significant (P<0.005) treatment effect on disease incidence (Table 1). Compared to the checks, applications of Palladium and Proud 3 had significantly lower incidence ratings. Treatments had no effect on overall disease severity, which rated the percentage of foliage that was killed on a scale of 0 to 10, plant growth, or the rate of foliage dieback (Table 2). No phototoxicity was observed in this trial. Given that only low levels of disease developed during this trial, additional tests should be conducted to determine the effectiveness of these fungicides in providing acceptable disease control under higher disease pressure.
Ornamental Horticulture Program Research Report Form p 2 Researcher: Gary Chastagner and Annie DeBauw Date: 12/5/2012 Project Title: L-112; IR-4 Efficacy of Management Tools for Botrytis on London Lilies Protocol #: 12-017 PRnumbers: 30556, 30448, 30480, 30554, 31356, 31360, 31357, 31358, 30449, 31359, 31361, Results Table Please insert results table here. Include PRnumbers for each treatment if multiple PRnumbers are included in this summary. Please include product, active ingredient, and statistics. Table 1. Effect of fungicide treatments on disease incidence 1. Trt # TRT/PRODUCT PRnumbers Rate/ 100 gal Disease Incidence 2 Palladium 62.5 WG 30554 12 oz 2.3c 4 Proud 3 31356 4 qts 6.5b 11 V-10135 31359 16 oz 8.0ab 10 Tourney 50 WDG 31358 4 oz 8.3ab 5 Regalia 5O 31360 4 fl oz 8.3ab 9 Torque 3.6 SC 31357 8 fl oz 8.3ab 6 Trinity 2 SC 30449 12 fl oz 8.3ab 7 Pageant 38 WG 30480 14 oz 8.5ab 3 Disarm 480 sc 30448 8 fl oz 9.0a 8 Chipco 26019 30556 16 oz 9.0a 1 Check - - 9.3a 12 ZeroTol 31361 1 gal 9.5a 1 Disease incidence data taken on September 19, 2012.. Numbers followed by the same letter are not significantly different, P<0.05, Tukey s Studentized Range (HSD) Test. Table 2. Effect of fungicide treatments on foliage dieback, disease severity, and growth. Trt # TRT/PRODUCT PRnumbers Rate/ 100 gal Foliage Dieback 1 Disease Severity 1 Growth (cm) 2 2 Palladium 62.5 WG 30554 12 oz 8.3a 1.3a 14.2a 4 Proud 3 31356 4 qts 4.3a 4.0a 14.7a 11 V-10135 31359 16 oz 4.5a 4.3a 16.1a 10 Tourney 50 WDG 31358 4 oz 5.3a 3.5a 13.1a 9 Torque 3.6 SC 31357 8 fl oz 6.8a 2.5a 15.7a 6 Trinity 2 SC 30449 12 fl oz 6.5a 1.8a 14.2a 5 Regalia 5O 31360 4 fl oz 5.0a 3.8a 13.0a 7 Pageant 38 WG 30480 14 oz 4.0a 3.8a 15.0a 8 Chipco 26019 30556 16 oz 6.0a 3.0a 14.3a 3 Disarm 480 sc 30448 8 fl oz 5.3a 3.0a 17.8a 1 Check - - 4.8a 4.3a 15.3a 12 ZeroTol 31361 1 gal 5.0a 4.5a 15.5a 1 Disease severity and foliage dieback on October 4, 2012 2 Numbers represent the difference between average plant heights taken on June 11 and July 26, 2012. 3 Numbers in columns were not significantly different, ANOVA Test.
Ornamental Horticulture Program Research Report Form p 3 Researcher: Gary Chastagner and Annie DeBauw Date: 12/5/2012 Project Title: L-112; IR-4 Efficacy of Management Tools for Botrytis on London Lilies Protocol #: 12-017 PRnumbers: 30556, 30448, 30480, 30554, 31356, 31360, 31357, 31358, 30449, 31359, 31361, Materials & Methods/Recordkeeping Please fill out the information below or attach a separate document with comparable information. Name(s) of Personnel Conducting Research: Gary Chastagner and Annie DeBauw Location of Trial (city/state): Washingtong State University Puyallup Research and Extension Center Use Site (greenhouse/shadehouse/field container/etc): Field Plot Crop History Crop Cultivar/Variety: Planting cell: Pot size & spacing: Row spacing: Asiatic hybrid Lily, > 10 cm/ London 3 feet of row per treatment 3 feet 40 centers Product(s) applied prior to start of experiment: Product Rate Application Type Date of Application Crop Growth Stage Apex 14-14-14 438 lbs/ acre In-furrow 4/12/2012 Prior to planting Terraclor 75 WP 3 lbs/ 1000 ft of row Application Volume 15.2 g/ foot of row In-furrow 4/12/2012 Prior to hilling 408 g/ 3 gal Diuron 80 WDG 2 lb/ac Broadcast 4/17/2012 After hilling 70.6 g / 2 L Round-up Pro Max 1.5% Spot spray 5/30/2012 Vegetative 2 fl oz / gal Ranger Pro 1.5% Spot spray 8/7/12 Flowering 2 fl oz/ gal Add more rows as needed.
Ornamental Horticulture Program Research Report Form p 4 Researcher: Gary Chastagner and Annie DeBauw Date: 12/5/2012 Project Title: L-112; IR-4 Efficacy of Management Tools for Botrytis on London Lilies Protocol #: 12-017 PRnumbers: 30556, 30448, 30480, 30554, 31356, 31360, 31357, 31358, 30449, 31359, 31361, Experiment Information Experimental Design: Randomized Split Block Number of Reps: 4 Materials & Methods: Insert materials & methods here only if information is not presented elsewhere. Include any changes from protocol. Materials & Methods: Bulbs used in this field trial were planted on April 12, 2012. Plot layout consisted of 5 rows spaced on 40 centers. Each row contained ten 3-foot-long planting cells with 3 feet between the planted cells. Prior to planting, all cells were fertilized by incorporating Apex 14-14-14 fertilizer @ 438 lbs. per acre in an 8 to 12-inch wide band just below the soil surface within the rows where the bulbs were to be planted. After placing 48 bulbs in each planting cell, bulbs were treated with an in-furrow 18 wide band of Terraclor at 3 lbs/1000 ft of row prior to hilling to control soil-borne diseases. Treatments were applied on 7, 10 and 14 day intervals starting on June 11, 2012 (Day 0). The average initial height of the plants was taken on Day 0. Three plants from each cell were randomly selected and the height of the plants was measured from the soil line to the top of the plant. Height data was taken again on July 25, 2012 (Day 45) when flowers were in full bloom. Overall disease incidence of whole cells was taken on a bi-weekly basis starting from June 11, 2012 to September 19, 2012 (Day 100). A final disease severity rating and foliage dieback rating was taken on October 4, 2012 (Day 115). Since this was the first time lilies had been planted in this location inoculum levels were very low. To facilitate disease development, inoculum was sprayed onto the foliage using a backpack sprayer on June 22 and July 25, 2012. Inoculum was prepared by growing Botrytis elliptica on potato dextrose agar. Once plates were fully colonized it was mixed in a blender with water and then strained through cheese cloth into the backpack sprayer. On the first inoculation, each cell received 100 mls of the inoculum suspension with a spore count of 2500 spores per ml. On the second inoculation, each cell received 120 mls inoculum suspension with a spore count of 182,000 spores per ml solution. Starting on July 18, 2012, the crop was periodically irrigated either by overhead irrigation or via a mist drip-line (starting August 17, 2012) as often as possible to assist with disease development. (See L-112; IR-4 Botrytis Lily file for irrigation intervals).
Ornamental Horticulture Program Research Report Form p 5 Researcher: Gary Chastagner and Annie DeBauw Date: 12/5/2012 Project Title: L-112; IR-4 Efficacy of Management Tools for Botrytis on London Lilies Protocol #: 12-017 PRnumbers: 30556, 30448, 30480, 30554, 31356, 31360, 31357, 31358, 30449, 31359, 31361, Product(s) applied during experiment (including treatments, fertilizers, etc): PRNUMBER PRODUCT/CODE S COMMON NAME MANUFACTURER Disarm 480 SC 30448 fluoxastrobin OHP Pageant 38 WG 30480 pyraclostrobin & boscalid BASF Palladium 62.5 WG 30554 cyprodinil & fludioxinil Syngenta Torque 3.6 SC 31357 tebuconazole Cleary Chemicals Tourney 50 WDG 31358 metconazole Valent Trinity 2 SC 30449 triticonazole BASF Proud 3 31356 thyme oil Bio Huma Netics, Inc. Regalia 31360 extract of Reynoutria sachalinensis Marrone Bio Invovations Chipco 26019 30556 iprodione Bayer Environmental Science V10135 SC 31359 fenpyrazamine Valent ZeroTol 31361 hydrogen peroxide BioSafe Table 3. Botrytis Treatment Rates and Application Dates. Trt # TRT/PRODUCT PRnumbers Rate/ 100 gal Application Date 1 Application Interval 1 check - - 2 Palladium 62.5 WG 30554 12 oz 1, 2, 4, 6, 8, 9, 11, 12, 13, 14, 16, 18, 19 7 day 10 Tourney 50 WDG 31358 1 oz 1, 3, 7, 10, 13, 15,19 14 day 3 Disarm 480 SC 30448 8 fl oz 1, 3, 7, 10, 13, 15,19 14 day 6 Trinity 2 SC 30449 12 fl oz 1, 3, 7, 10, 13, 15,19 14 day 4 Proud 3 31356 4 qts 1, 2, 4, 6, 8, 9, 11, 12, 13, 14, 16, 18, 19 7 day 5 Regalia 5O 31360 4 fl oz 1, 2, 4, 6, 8, 9, 11, 12, 13, 14, 16, 18, 19 7 day 7 Pageant 38 WG 30480 14 oz 1, 3, 7, 10, 13, 15,19 14 day 8 Chipco 26019 30556 16 oz 1, 3, 7, 10, 13, 15, 19 14 day 9 Torque 3.6 SC 31357 8 fl oz 1, 3, 7, 10, 13, 15,19 14 day 11 V-10135 31359 16 oz 1, 2, 4, 6, 8, 9, 11, 12, 13, 14, 16, 18, 19 7 day 12 ZeroTol 31361 1 gal 1, 2, 5, 8, 10, 12, 14, 17, 19 10 day 1 Dates: 1 = 6/11/12, 2 = 6/20/12, 3 = 6/25/12, 4 = 6/27/12, 5 = 6/29/12, 6 = 7/5/12, 7 = 7/9/12, 8 = 7/12/12, 9 = 7/19/12, 10 = 7/24/12, 11 = 7/26/12, 12 = 8/2/12, 13 = 8/9/12, 14 = 8/17/12, 15 = 8/23/12, 16 = 8/24/12, 17 = 8/28/12, 18 = 8/31/12, 19 = 9/7/12 Photos See L-112; IR-4 Botrytis Lily Photos file for photos of field layout and treatment effects.
Ornamental Horticulture Program Research Report Form p 6 Researcher: Gary Chastagner and Annie DeBauw Date: 12/5/2012 Project Title: L-112; IR-4 Efficacy of Management Tools for Botrytis on London Lilies Protocol #: 12-017 PRnumbers: 30556, 30448, 30480, 30554, 31356, 31360, 31357, 31358, 30449, 31359, 31361, Data Collected Please describe data collected and scoring system. Also include the dates data were collected. Disease incidence was rated on a scale of 0 to 10 where 0 = none, 1 = 1-10%, 2 = 11-20%,.., and 10 = 91-100% of plants were diseased. Incidence ratings were taken on days 0, 10, 17, and 25. Disease severity was rated on a scale of 0 to 10 where 0 = none, 1 = 1-10%, 2 = 11-20%,.., and 10 = 91-100% of the foliage on the plants was killed. Foliage dieback was rated on a scale of 0 to 10 where 0= no green foliage, 1 = 1-10% green foliage, 2 = 11-20% green foliage, 10 = 91-100% green foliage remaining on plants. Disease incidence ratings were taken on days 28, 43, 41, 60, 73, 88, and 100. Disease severity and foliage dieback were taken on day 115. Growth data was obtained by measuring the initial height of the plants on June 11, 2012 (Day 0). Height data was collected a second time when crop was in full bloom on July 26, 2012 (Day 45). When taking height data, 3 plants from each cell were randomly selected and the height of the plants was measured from the soil line to the top of the plant. The growth between Day 0 and Day 45 was then calculated. Data analysis: Data were analyzed using ANOVA (GLM SAS). Treatment means were separated using Tukey s Studentized Range (HSD) Test. Application Equipment: During the growing season, weeds were controlled with a combination of Diuron 80 WDG @ 2 lbs./acre as well as broadcast and spot applications of Round-Up ProMax and Ranger Pro using a Solo backpack sprayer. For broadcast applications, sprayer was equipped with a Spray Systems TK 2 nozzle. For spot applications sprayer was equipped with a 8002 Tee-jet nozzle. For both types of weed control applications the sprayer was set at 15 psi in the indicated volumes of water. All trial treatment applications were applied with a CO 2 sprayer equipped with an 8002 Tee Jet nozzle at 15 psi in the indicated volume of water. Raw Data Insert raw data below or send separate file containing raw data. See L-112; IR-4 Botrytis Lily file for raw data Environmental conditions during the experiment: Insert temperature, precipitation and/or irrigation, and relative humidity with a minimum of high, low and average daily temperatures. Or send separate file with this information. Include a statement about any significant weather or environmental events during the course of the experiment. (see L-112; IR-4 Botrytis Lily file for environmental data).