Plant Propagation Protocol for Ribes Watsonianum ESRM 412 Native Plant Production Spring 2009

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Plant Propagation Protocol for Ribes Watsonianum ESRM 412 Native Plant Production Spring 2009 Figure 1: Ribes Watsonianum, Kittitas County, WA (Curtesy of Ben Legler @ the Burke Museum of Natural History and Culture) (Legler, 2004) Family Names Family Scientific Name: Family Common Name: Grossulariaceae Currant family TAXONOMY

Scientific Names Genus: Species: Species Authority: Variety: Sub species: Cultivar: Authority for Variety/Subspecies: Common Synonym(s) (include full scientific names (e.g., Elymus glaucus Buckley), including variety or subspecies information) Common Name(s): Species Code (as per USDA Plants database): Geographical range (distribution maps for North America and Washington state) Ribes watsonianum Koehne Grossularia watsoniana (Koehne) Coville & Britt. Spring Gooseberry, Spiny Gooseberry, Watson Gooseberry GRWA GENERAL INFORMATION Distribution: Northwestern U.S.A.: United States Oregon, Washington

Figure 2: Ribes Watsonianum distribution. Shaded present, white absent. (Cortesy: USDA plant database). (United States Department of Agriculture, 2009) Figure 3: RIWA in the state of Washington Shaded present, white absent. (Courtesy of (United States Department of Agriculture, 2009)

Ecological distribution (ecosystems it occurs in, etc): Climate and elevation range Local habitat and abundance; may include commonly associated species Plant strategy type / successional stage (stress tolerator, competitor, weedy/colonizer, seral, late successional) Plant characteristics (life form (shrub, grass, forb), longevity, key characteristics, etc) Propagation Goal (Options: Plants, Cuttings, Seeds, Bulbs, Somatic Embryos, and/or Other s): Propagation Method (Options: Seed or Vegetative): Habitat: moist woods, sunny edges of streams and margins of meadows in mountains. (Flora of the Inland Pacific Northwest, 2000) Prefers moist soils. It can grow in semi shade (light woodland) or no shade. As one of the many Ribes species, Ribes Watsonianum serves as the alternate host to white pine blister rust, a disease that has had a severe impact on forest management. Generally it is a low growing shrub. It flowers in May June, and the seeds ripen from July to August. The flowers are hermaphrodite and are pollinated by Insects. Plants Seed PROPAGATION DETAILS (Forest Service, 1948) (if other is not noted)

Figure 4: R. Watsonianum seeds. (Curtesy of (Encyclopedia of Life, 2009) Product Type (options: Container (plug), Bareroot (field grown), Plug + (container field grown hybrids, and/or s (seeds, cuttings, poles, etc.)) Stock Type: Time to Grow (from seeding until plants are ready to be outplanted): Target Specifications (size or characteristics of target plants to be produced): Collection (how, when, etc): Note: Plant could be propagated by Bare Root, Container, Seed, Cuttings, Layering and Micropropagation. Container (plug) The plants should be 5 6 cm in 6 7 weeks after germination. Prick out the seedlings into individual pots when they are large enough to handle and grow them on in a cold frame for their first winter, planting them out in late spring of the following year. (Plants For A Future, 2000) To minimize consumption by birds the fruit should be picked or stripped from the branches as soon as possible after ripening. The fruit should be spread out to dry. To prevent overheating the fruit should be spread out into shallow layers.

Processing/Propag ule Characteristics (including seed density (# per pound), seed longevity, etc): Pre Planting Treatments (cleaning, dormancy treatments, etc): Growing Area Preparation / Annual Practices for Perennial Crops (growing media, type and size of containers, etc): Establishment Phase (from seeding to germination): Length of Establishment Active Growth Phase (from germination until plants are no longer actively growing): Length of Active Growth Hardening Phase (from end of active growth phase to end of growing season; primarily related to the development of To extract seeds one should macerate the berries in water. The empty seeds and pulp should flow away, while the filled seeds should be dried and run through the fan to remove any debris. The best way to store the seeds: low initial moisture content, at low temperature and in the sealed containers. Stored seed requires 4 5 months cold stratification at between 0 to 9 c and should be sown as early in the year as possible (Baskin & Baskin, 1998). Under normal storage conditions the seed can remain viable for 17 years or more. Natural germination occurs in the following spring after dispersal. Germination could be increased by cold stratification in sand or peat preceded by stratification at warm temperatures. It is also possible to scarify the seeds mechanically or use a short period acid treatment.

cold hardiness and preparation for winter): Length of Hardening Harvesting, Storage and Shipping (of seedlings): Length of Storage (of seedlings, between nursery and outplanting): Guidelines for Outplanting / Performance on Typical Sites (eg, percent survival, height or diameter growth, elapsed time before flowering): Other Comments (including collection restrictions or guidelines, if available): Propagation Goal (Options: Plants, Cuttings, Seeds, Bulbs, Somatic Embryos, and/or Other s): Propagation Method (Options: Seed or Vegetative): Product Type (options: Container (plug), Bareroot (field grown), Plug + (container field grown hybrids, and/or s The plants commence bearing in 5 years from seed and 2 years from cuttings (California Rare Fruit Growers, Inc., 1996). Plants Seed PROPAGATION DETAILS (Young & Young, 1992) Barefoot (field grown)

(seeds, cuttings, poles, etc.)) Stock Type: Time to Grow (from seeding until plants are ready to be outplanted): Target Specifications (size or characteristics of target plants to be produced): Collection (how, when, etc): Processing/Propagul e Characteristics (including seed density (# per pound), seed longevity, etc): Pre Planting Treatments (cleaning, dormancy treatments, etc): Growing Area Preparation / Annual Practices for Perennial Crops (growing media, type and size of containers, etc): Establishment Phase (from seeding to germination): Length of Establishment Active Growth Phase (from germination until plants are no longer actively Seeds are usually sown in the fall at the rate of 630 to 840 per m 2 and covered with 0.6 am of soil. From a kilogram of seed about 400 seedlings are produced. To minimize the loss to the birds the fruit should be collected as soon as they are ripe. The fruit can be macerated and the seeds recovered by flotation. The seeds are highly dormant and require warm stratification after prolonged prechilling. Prechilling could be for up to 6 month.

growing): Length of Active Growth Hardening Phase (from end of active growth phase to end of growing season; primarily related to the development of cold hardiness and preparation for winter): Length of Hardening Harvesting, Storage and Shipping (of seedlings): Length of Storage (of seedlings, between nursery and outplanting): Guidelines for Outplanting / Performance on Typical Sites (eg, percent survival, height or diameter growth, elapsed time before flowering): Other Comments (including collection restrictions or guidelines, if available): Propagation Goal (Options: Plants, Cuttings, Seeds, Bulbs, Somatic Embryos, and/or Other s): PROPAGATION DETAILS (US Department of Agriculture, 1974) Plants

Propagation Method (Options: Seed or Vegetative): Product Type (options: Container (plug), Bareroot (field grown), Plug + (container field grown hybrids, and/or s (seeds, cuttings, poles, etc.)) Stock Type: Time to Grow (from seeding until plants are ready to be outplanted): Target Specifications (size or characteristics of target plants to be produced): Collection (how, when, etc): Processing/Propag ule Characteristics (including seed density (# per pound), seed longevity, etc): Pre Planting Treatments (cleaning, dormancy treatments, etc): Seeds Barefoot (field sown) The fruit should be picked or stripped from the branches as soon as possible after ripening to minimize consumption by birds. Unless the seed is to be extracted immediately, it should be spread out to dry. To prevent overheating the fruit should be spread out into shallow layers. To extract seeds one should macerate the berries in water. A fast method: put small quantities of the berries in the kitchen blender, cover the berries with water, and run blender for 15 to 45 seconds. The blender should run long enough to separate the sound seed from the pulp. Then one should add more water and allow the sound seed to settle. The empty seeds and pulp should flow away, while the filled seeds should be washed into a funnel lined with filter paper. Then the seed should be dried. Germination could be increased by cold stratification in sand or peat, or in a mixture of these media preceded by stratification at warm temperatures. It is also possible to scarify the seeds mechanically or use a short

Growing Area Preparation / Annual Practices for Perennial Crops (growing media, type and size of containers, etc): Establishment Phase (from seeding to germination): period acid treatment. The best seedbed appears to be mineral soil well supplied with humus. Usually Ribes seed are sown in the fall, but it also could be stratified and sown in the spring. If sown in spring, the seed should be stratified. Nonetheless the general recommendation is to fallsaw whenever possible. Length of Establishment Active Growth Phase (from germination until plants are no longer actively growing): Length of Active Growth Hardening Phase (from end of active growth phase to end of growing season; primarily related to the development of cold hardiness and preparation for winter): Length of Hardening Harvesting, Storage and Shipping (of seedlings): Length of Storage (of seedlings, between nursery and outplanting): Guidelines for Outplanting / Performance on

Typical Sites (eg, percent survival, height or diameter growth, elapsed time before flowering): Other Comments (including collection restrictions or guidelines, if available): Propagation Goal (Options: Plants, Cuttings, Seeds, Bulbs, Somatic Embryos, and/or Other s): Propagation Method (Options: Seed or Vegetative): Product Type (options: Container (plug), Bareroot (field grown), Plug + (container field grown hybrids, and/or s (seeds, cuttings, poles, etc.)) Stock Type: Time to Grow (from seeding until plants are ready to be outplanted): Target Specifications (size or characteristics of PROPAGATION DETAILS Other methods Cuttings (Hartmann, Kestler, Davies, & Geneve, 2002) (if other is not noted) Cutting Vegetative Gooseberries root with more difficulty, that currants, but they still could be propagated by collecting hardwood cuttings 20 to 25 cm (8 to 10 in) long in late fall, stored in moist sand, sawdust, or peat moss at about 2 C for precallusing them at low temperatures over the winter, and then planted in the spring. The plants can be transplanted to theior permanent location in one or two years, depending upon their growth..

target plants to be produced): Collection (how, when, etc): Processing/Propag ule Characteristics (including seed density (# per pound), seed longevity, etc): Pre Planting Treatments (cleaning, dormancy treatments, etc): Growing Area Preparation / Annual Practices for Perennial Crops (growing media, type and size of containers, etc): Establishment Phase (from seeding to germination): Length of Establishment Active Growth Phase (from germination until plants are no longer actively growing): Length of Active Growth Hardening Phase (from end of active growth phase to end of growing season; primarily related to the development of cold hardiness and

preparation for winter): Length of Hardening Harvesting, Storage and Shipping (of seedlings): Length of Storage (of seedlings, between nursery and outplanting): Guidelines for Outplanting / Performance on Typical Sites (eg, percent survival, height or diameter growth, elapsed time before flowering): Other Comments (including collection restrictions or guidelines, if available): Propagation Goal (Options: Plants, Cuttings, Seeds, Bulbs, Somatic Embryos, and/or Other s): Propagation Method (Options: Seed or Vegetative): Product Type (options: Container (plug), Bareroot (field grown), Plug + (container field grown hybrids, and/or s (seeds, cuttings, PROPAGATION DETAILS (Hartmann, Kestler, Davies, & Geneve, 2002) Plants Vegetative Barefoot

poles, etc.)) Stock Type: Time to Grow (from seeding until plants are ready to be outplanted): Target Specifications (size or characteristics of target plants to be produced): Collection (how, when, etc): Processing/Propag ule Characteristics (including seed density (# per pound), seed longevity, etc): Pre Planting Treatments (cleaning, dormancy treatments, etc): Growing Area Preparation / Annual Practices for Perennial Crops (growing media, type and size of containers, etc): Establishment Phase (from seeding to germination): Length of Establishment Active Growth Phase (from germination until plants are no longer actively growing): Length of Active Mound layering is used for gooseberries. Shoots usually root well after one season. They are then cut off and transferred to the nursery row for a second season s growth before they are set out in their permanent location.

Growth Hardening Phase (from end of active growth phase to end of growing season; primarily related to the development of cold hardiness and preparation for winter): Length of Hardening Harvesting, Storage and Shipping (of seedlings): Length of Storage (of seedlings, between nursery and outplanting): Guidelines for Outplanting / Performance on Typical Sites (eg, percent survival, height or diameter growth, elapsed time before flowering): Other Comments (including collection restrictions or guidelines, if available): References (full citations): Other Sources Consulted (but that contained no pertinent information) (full citations): Protocol Author Note: Gooseberries can also be easily micropropagated and the rooted plantlets stored under refrigeration for as long as 130 days with 100 percent survival. INFORMATION SOURCES See Below See Below Yana Kazak

(First and last name): Date Protocol Created or Updated (MM/DD/YY): 4/28/2009 Bibliography Baskin, C., & Baskin, J. (1998). Seeds. Ecology, Biogeography, and Evolution of Dormacy and Germination. New York: Academic Press. California Rare Fruit Growers, Inc. (1996). Retrieved April 28, 2008, from GOOSEBERRY: http://www.crfg.org/pubs/ff/gooseberry.html Culture, B. M. (2004, June 14). Burke Museum of Natural History and Culture. Retrieved April 23, 2009, from Ribes watsonianum: http://biology.burke.washington.edu/herbarium/imagecollection.php Encyclopedia of Life. (2009, April 25). Retrieved April 25, 2009, from Ribes : https://eol.org Flora of the Inland Pacific Northwest. (2000). Retrieved April 25, 2009, from Ribes : http://www.cwnp.org/ Forest Service, U. D. (1948). Woody-Plant Seed Manual. Washington, D.C.: United States Government Printing Office. Hartmann, H., Kestler, D., Davies, F., & Geneve, R. (2002). Plant Propagation: Principles and Practices. Upper Saddle River, New Jersey: Prentice Hall. Legler, B. (2004, June 14). Ribes watsonianum. 13 miles north of Ellensburg, Kittitas County, WA, USA. Plants For A Future. (2000). Retrieved April 25, 2009, from Database Search : http://www.ibiblio.org/ United States Department of Agriculture. (2009, April 23). Retrieved April 23, 2009, from Natural Resources Concervation Service, Plants Profile: http://plants.usda.gov/java/profile?symbol=riwa United States Department Of Agriculture, F. S. (1988). Range Plant Handbook. New York: Dover Publications, Inc. US Department of Agriculture, F. S. (1974). Seeds of Woody Plants in the United States. Washington, D.C.: United States Government Printing Office. Young, J., & Young, C. (1992). Seeds of Woody Plants in North America. Partland, Oregon: Dioscorides Press. Note: This template was modified by J.D. Bakker from that available at: http://www.nativeplantnetwork.org/network/sampleblankform.asp