TESCAN MIRA FESEM Presentation Global Supplier of Scanning Electron Microscopes

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TESCAN MIRA FESEM Presentation Global Supplier of Scanning Electron Microscopes TESCAN USA 508 Thomson Park Drive Cranberry TWP, PA 16066

Outline About TESCAN Product Portfolio MIRA FESEM Overview Electron Optics Various Models Features EDS Analysis Imaging Results Value & Excellence in SEMs

About TESCAN Established: 1991 Location: Brno, Czech Republic Field of Activity: Research, development, manufacturing and worldwide supply of scanning electron microscopes and related products Main Products: Scanning electron microscopes High resolution Schottky FE-SEMs Focused ion beam SEMs Detectors and accessories for SEMs Nanotechnology Instrumentation Value & Excellence in SEMs

TESCAN Product Line 2012 Over 1100 SEMs all over the world since 2012 since 2008 since 2009 since 2007 since 1999 since 2005 EasyProbe TIMA 4

About TESCAN Fig. Brno International Trade Fair Center Historical Background Brno, Czech Republic The second largest city in the Czech Republic Traditional center of industry and commerce and Central European trade fairs 6 Universities with 27 faculties and about 80,000 students Origin of world-famous researchers 15 Institutes of Academy of Science Electron Microscopy in Brno Over 60 years tradition of electron microscopy in Brno Former leading supplier of EM for Eastern Europe Location of other SEM manufacturers Fig. First Czechoslovak electron microscope Tesla BS241 (1951), Tesla BS242 (1954)

About TESCAN Historical Facts The first TEM assembled at the Technical University of Brno at the end of the 1940's TESLA Brno - introduced the first commercial TEM in 1953 - the leading manufacturer of Electron-Optical Devices in Eastern Europe in the 2 nd half of the 20 th century The Institute of Scientific Instruments of the Academy of Science of the Czech Republic was founded in 1956 The Tesla BS-242 TEM was awarded the gold medal at the World Exhibition in Brussels in 1958 Tesla Brno manufactured over 4,000 devices (TEM + SEM) during four decades of its existence TESCAN founded by former engineers and managers of Tesla Brno (Jaroslav Klima, former head of Tesla s SEM Division) TESCAN, a.s. is one of the companies successfully continuing the electron microscopy tradition in Brno

TESCAN Team: more than 160 employees From R&D to a Final Product

TESCAN today TESCAN are total about 4500 m 2 Mechanical Workshops 480 m 2 Electrical Workshops 265 m 2 Assembling 200 m 2 Stores 160 m 2 Final Assembling (clean rooms) 350 m 2 Packaging 300 m 2 Clean R&D laboratiories 70 m 2 Demonstration laboratiories Standard 30 m 2 Clean 40 m 2 R&D offices 150 m 2 Sales, logistics, economics and company management offices 680 m 2 Meeting rooms 395 m 2

TESCAN Future and Strengths TESCAN, a.s. 20 Years of Tradition and Excellence in Scanning Electron Microscopy New premises Modern manufacturing facilities Strong research and development Clean rooms for assembling Educated and experienced team Worldwide sales and service network

About TESCAN - Basic Info North America Headquarters Location: Cranberry Township, PA (Pittsburgh, PA) West Coast Demonstration Lab Location: Pleasanton, California

International References

TESCAN USA CUSTOMERS Jacksonville Orlando TESCAN provides a guaranteed 48 hour response time (Our Goal is 24 hours) & guaranteed 95% uptime.

TESCAN USA CUSTOMERS RIO TINTO US Steel Industries TESCAN provides a guaranteed 48 hour response time (Our Goal is 24 hours) & guaranteed 95% uptime.

Specific Features Value & Excellence in SEMs High resolution Schottky FEG-SEM Unique Wide Field Optics SEM optics design Intermediate lens (IML) for the beam aperture optimization Uniform energy In-Flight Beam Tracing Stereoscopic imaging 3D Beam Technology UniVac - variable pressure version Beam Deceleration Large Chamber and Stage Capability Variable Pressure Optional / Accessories / Software modules On axis In-Column SE and BSE Detector for high resolution at lower kv STEM Detector Low Vacuum Secondary TESCAN Detector (LVSTD) 16k x 16k Image Store as standard Beam Blanker Load Lock Peltier Cooling Stage Nanomanipulators, etc.

SEM Column High Resolution Schottky FEG SEM Point source high brightness Schottky emitter Convergent beam, uniform energy,minimized aberrations Wide Field Optics Unique Tescan three-lens column design optimized for FE source IML - Intermediate lens for beam aperture optimization Automated alignment In-Flight Beam Tracing Original control of the beam properties

Wide Field Optics TM Tescan SEMs = World s Largest Magnification Range From Centimeters Unique Wide Field Mode (in centimeters) Extra-low magnification (down to 1,2x) Extra wide scanning angle (up to 45 ) to Nanometers High Resolution Shottky FE-SEM (1.2 nm) Figs. Magnification up to 1,000,000x Top: Defining areas for automated analysis Bottom: High resolution test

Wide Field Optics Range of Scanning Modes Resolution Depth Field Wide Field Channelling 17

Principles of the beam deceleration Principles of the beam deceleration: Applying negative voltage on the specimen Using higher accelerating voltage of primary beam that is decelerated just before its landing on the specimen Results in smaller chromatic aberration = better resolution Example: Primary beam energy = 5 kev Specimen bias voltage = -4 kv Landing energy = 5 4 = 1 kev Special feature: In-Flight Beam Tracing TM controls precise focus in DM Magnetic field Electrostatic field equipotentials Primary beam Magnetic objective lens at 0V Sample biased -3 to -5 kv Value & Excellence in SEMs

Accelerated secondary electrons Annular scintillator detector Principles of the secondary electron detection: negative voltage on the specimen accelerates SE towards and inside of the column The secondary electron are focused and condensed close to the optical axis acceleration field gives to SE enough energy to be detected by a BSE-like-detector (high efficiency YAG:Ce) Example: Specimen bias voltage = -4 kv SE original energy = about 4 ev SE detector landing energy = 4+ kev Value & Excellence in SEMs

In-beam BSE detector Annular scintillator detector Sample at the ground potential High angle back-scattered electrons detected by in-beam detector (same detector as for the deceleration mode) Complementary signal to the standard (below pole piece) BSE detector that detects rather low angle back-scattered electrons Enables to use very short WD Frees space under objective lens for other detectors Backscattered electrons Sample at ground potential Value & Excellence in SEMs Blast Furnace Slug (FE/Si/Al/Zr)

BDM low kv resolution Resolution test Au-C, 1kV In-Beam SE detector Advantages of the beam deceleration mode: achieving very low landing voltages down to 100 V reduced beam damage of sensitive samples much better resolution at low and ultra-low voltages 1.5 nm @ 3 kv < 2.0 nm @ 1 kv 2.5 nm @ 200 V 1kV In-Beam SE Deceleration Mode detector Value & Excellence in FIB-SEMs

Resolution Test

Used AFM tip: HiVac, 5 kv, SE detector SE Detector Opal: HiVac, 30 kv, SE detector, mag. 230 kx

In-Beam Detector Special detector position (in lens) Detects SE which are emitted back into the objective Allows specimen examination at very short working distance Outstanding resolution (1 nm at 30 kv, 2 nm at 3 kv) Available only for MIRA3 FEG-SEM Ag nanoclusters: HiVac, 30 kv, In-Beam detector, mag. 217 kx Value & Excellence in SEMs

In-Beam Detector Nanofibers (gold coated): HiVac, 30 kv, In-Beam detector, mag. 300 kx CdTe nanostructures: HiVac, 30 kv, In-Beam detector

In-Beam Detector Golden nanoparticles: HiVac, 30 kv, In-Beam detector, mag. 867 kx Nonwoven textile: HiVac, 5 kv, In-Beam detector

In-Beam Detector TiO: HiVac, 15 kv, In-Beam detector Carbon nanotubes (Pt coated): HiVac, 30 kv, In-Beam detector, mag. 189 kx

BSE Detector YAG scintillator detector Equipped with first-class single-crystal YAG scintillator High efficiency low noise Fast imaging rate High resolution (2 nm at 30 kv) High sensitivity and atomic number resolution (0.1 Z) Retractable (manual/motorized) version Semiconductor with metalic bridge: HiVac, 5 kv, BSE detector 4-Quadrant semiconductor detector It can achieve signal from 4 quadrants separately and/or mix them Used to get both compositional and topographical contrast Value & Excellence in SEMs

STEM Detector Ultrastructure of samples observed in SEM Suitable for life science, material science, nanotechnology, etc. High magnification High resolution (0,8 nm at 30 kv) Sample preparation techniques same as for TEM Good contrast of images achieved without staining Simultaneous dark field and bright field imaging Cross section of the rat intestine: HiVac, 30 kv, STEM detector (bright field) Value & Excellence in SEMs

STEM Detector Ash: HiVac, 30 kv, STEM detector (bright field), mag. 396 kx Immunolabeling (10 nm gold nanoparticles): HiVac, 25 kv, STEM detector (bright field)

LVSTD Low Vacuum Secondary TESCAN Detector Original design Convenient for non-conductive samples investigation Modified Everhart-Thornley design - patented by TESCAN True secondary electron detecting in low vacuum condition Microlens differential barrier Blue- green mold (Penicillium Roqueforti): UniVac, 20 Pa, Water Vapor, -36 C, 20 kv, LVSTD Value & Excellence in SEMs

Value & Excellence in SEMs CdTe nanostructures: HiVac, 30 kv, SE detector

Please contact us if you have any questions about analyses or further requirements. Contact: Drew Erwin www.tescan.com Streptococcus mutans (gold coated): HiVac, 10 kv, In-beam detector Value & Excellence in SEMs

TESCAN, a.s. Libušina tř. 21 623 00 Brno Czech Republic Europe www.tescan.com www.tescan.cz Contact: E-mail: info@tescan.cz tel: +420 547 130 414 Value & Excellence in SEMs