ULT Freezer Model 5700 Series Chest Freezer Manual Number Rev. 15

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Transcription:

ULT Freezer Model 5700 Series Chest Freezer Manual Number 7005708 Rev. 15

Preface Models Covered Models Capacity in Cubic Feet 5708 3 120 5709 3 230 5710 6.7 120 5711 6.7 230 5712 12.7 230 5715 12.7 120 5718 17 120 5719 17 230 5720 20 230 5721 20 120 Voltage MANUAL NUMBER 7005708 15 23939 5/29/07 Added note - adjust LN2 tank pressure relief valve to 30 PSI maximum blow-off ccs -- 24100 5/29/07 Clarified battery switch to Standby mode with symbol ccs 14 23859/FR-1940 12/4/06 Clarified BUS install (probe/solenoid harness) instructions ccs 13 23700/Fr-1919 10/09/06 Updated safety temp specs (from 5-40 C to 5-43 C) ccs 12 23579/FR-1911 8/18/06 Corrected start capacitor part number on electrical schematic ccs 11 23170 2/13/06 Remote alarm connector is customer installed aks 10 23062 12/30/05 Changed display boards for 3 and 6.7 cu. ft. models aks -- 22930 12/30/05 WEEE Directive aks 9 22895 8/17/05 Removed rotalock valves from compressors aks 8 22676 5/23/05 Changed control panel alarm indicators aks 7 22562 3/7/05 Modified software to allow alarms within 5C of set point aks -- 22769 3/7/05 Changed circuit and breaker requirements for 5708 and 5710 aks 6 22362 9/27/04 Changed micro board aks 5 22136 5/6/04 Revised electrical schematics aks 4 22179/22216 3/16/04 Revised electrical schematics aks 3 21800 9/16/03 Revised refrigeration schematics aks 2 21795 8/15/03 Revised BUS operation instructions aks 1 21605 5/27/03 Wrong power alarm modifications aks -- 21526 5/27/03 Solenoid valve mounting bracket aks 0 -- 3/12/03 Original Manual aks REV ECR/ECN DATE DESCRIPTION By VWR International 7005708 i

Preface Packing List Part Number Description Quantity 34040 Key Ring 1 213F Key 2 380520 Neoprene Cap 2 510016 1/4-20 x 5-1/2 Bolt 2 402058 Vacu-Key 1 195763 Retaining Clip 1 370563 Remote Alarm Connector 1 Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual may result in damage to the unit, injury to operating personnel, and poor equipment performance. Caution All internal adjustments and maintenance must be performed by qualified service personnel. Material in this manual is for information purposes only. The contents and the product it describes are subject to change without notice. VWR International makes no representations or warranties with respect to this manual. In no event shall VWR be held liable for any damages, direct or incidental, arising out of or related to the use of this manual. 2003 Thermo Fisher Scientific. All rights reserved. ii 7005708 VWR International

Preface Important operating and/or maintenance instructions. Read the accompanying text carefully. Potential electrical hazards. Only qualified persons should perform procedures associated with this symbol. Equipment being maintained or serviced must be turned off and locked off to prevent possible injury. Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be damaged by elevated temperatures. Marking of electrical and electronic equipment, which applies to electrical and electronic equipment falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market after 13 August 2005. This product is required to comply with the European Union s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo has contracted with one or more recycling/disposal companies in each EU Member State European Country, and this product should be disposed of or recycled through them. Further information on Thermo s compliance with this directive, the recyclers in your country and information on Thermo Fisher Scientific products will be available at www.thermofisher.com. Always use the proper protective equipment (clothing, gloves, goggles, etc.) Always dissipate extreme cold or heat and wear protective clothing. Always follow good hygiene practices. Each individual is responsible for his or her own safety. VWR International 7005708 iii

Preface Do You Need Information or Assistance on VWR Scientific Products? The VWR Sales Group can provide information on pricing and give you quotations. We can take your order and provide delivery information on major equipment items or make arrangements to have your local sales representative contact your. Our products are listed on the internet and we can be contacted through our Internet home page. 1-800-932-5000 VWR, Toll Free, US http://www.vwrsp.com Internet Worldwide Web Home page The VWR Product Service Group at Forma can supply technical information about proper setup, operation or troubleshooting of your equipment. We can fill your needs for replacement parts or provide you with on-site service. We can also provide you with a quotation on our Extended Maintenance Program for your Forma products. Whatever VWR or Forma products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service call. When more extensive service is necessary, we will assist you with direct factory trained technicians or a qualified service organization for on-the-spot repair. If your service need is covered by the VWR Brand warranty, we will arrange for the unit to be repaired at our expense and to your satisfaction. Regardless of your needs, VWR support by Forma's professional telephone technicians is available to assist you Monday through Friday from 8:00a.m. to 6:30 p.m. Eastern Time. Please call or fax us at: 1-740-373-4763 Direct 1-888-213-1790 Toll Free, U.S. and Canada 1-740-373-4189 FAX http://www.thermo.com/forma services.controlenv@thermo.com Internet Worldwide Web Home Page Service E-Mail Address If you wish to write, our mailing address is: Thermo Fisher Scientific 401 Millcreek Road, Box 649 Marietta, OH 45750 iv 7005708 VWR International

Table of Contents Section 1 Section 2 Section 3 Section 4 Installation and Start-up.....................................1-1 Control Panel Keys, Displays, Indicators....................1-5 Operation of the Keypad................................1-6 Install Freezer.........................................1-6 Choose Location.....................................1-6 Install Wall Bumpers...................................1-7 Remote Alarm Contacts................................1-7 Attach Power Cord...................................1-8 Connect Unit to Electrical Power.........................1-8 Freezer Start-Up.......................................1-9 Set Operating Temperature.............................1-9 Set High Temperature Alarm............................1-10 Set Low Temperature Alarm.............................1-10 Run Mode............................................1-11 Calibrate...................................................2-1 Calibrate Mode........................................2-1 Calibrate Control Probe................................2-1 Alarms....................................................3-1 Alarms..............................................3-1 Wrong Power Alarm....................................3-1 High Stage System Failure................................3-2 Probe Failure Alarm....................................3-2 Voltage Compensation Alarm.............................3-2 Maintenance...............................................4-1 Clean Cabinet Exterior..................................4-1 Clean Air Filter........................................4-1 Clean Condenser.......................................4-1 Clean Water-cooled Condenser...........................4-2 Defrost Chamber......................................4-2 Clean Lid Gasket......................................4-3 Replace Battery(s)......................................4-3 Prepare Unit for Storage..................................4-5 Preventive Maintenance.................................4-6 VWR International 7005708 v

Table of Contents Section 5 Section 6 Section 7 Section 8 Section 9 Section 10 Appendix A Appendix B Appendix C Factory Options.............................................5-1 BUS (Back Up System)..................................5-1 Install Injection Assembly...............................5-1 Install Temperature Probe...............................5-2 Connect Probe/Solenoid Harness.........................5-4 BUS Control Panel....................................5-4 Configure Optional BUS...............................5-5 Set Optional BUS Set Point.............................5-6 Disconnect Fitting Assembly, Transfer Hose.................5-6 Chart Recorder........................................5-7 Install Chart Paper....................................5-7 Recorder Calibration...................................5-7 Datalogger...........................................5-8 Water-cooled Condenser.................................5-9 Specifications..............................................6-1 Spare Parts.................................................7-1 Refrigeration Schematics....................................8-1 Electrical Schematics.......................................9-1 Warranty..................................................10-1 Handling Liquid Nitrogen....................................A - 1 Handling Liquid CO2.........................................B - 1 First Aid....................................................C - 1 vi 7005708 VWR International

Section 1 Installation and Start-up Figures 1-1 and 1-2 show the front view of the freezer and indicate the following freezer components: Control Panel - keypad, displays and indicators BUS (Optional Back Up System) panel Optional temperature recorder (7 day, one pen) or datalogger Key lock - keyed lid lock Figure 1-1. Front View Models 5708, 5709, 5710, 5711 Figure 1-2. Front View Models 5712, 5715, 5718, 5719, 5720, 5721 VWR International 7005708 1-1

Section 1 Installation and Start-up Figures 1-3 and 1-4 display the rear view of the freezer and indicate the following freezer components: Remote alarm contacts. Power inlet for power cord connection. Optional BUS connections for probe and solenoid. Power Switch (mains disconnect). Figure 1-3. Rear View Models 5708, 5709, 5710, 5711 Figure 1-4. Rear View Models 5712, 5715, 5718, 5719, 5720, 5721 1-2 7005708 VWR International

Section 1 Installation and Start-up The probe cover houses the control, optional recorder, datalogger, 1535 alarm, or BUS probes. Figure 1-5. Probe Cover Figures 1-6, 1-7, and 1-8 indicate the following components: Freezer filter location. Battery power switch (freezer and BUS). Thermocouple receptacle. Battery mounting bracket. Freezer and optional BUS battery. Figure 1-6. 12.7,17,20 cu.ft. Freezer Models 5712, 5715, 5718, 5719, 5720, 5721 VWR International 7005708 1-3

Section 1 Installation and Start-up Figure 1-7. Freezer Left Side - Sidecar panel removed Figure 1-8. 3, 6.7 cu. ft. Freezers - Models 5708, 5709, 5710, 5711 1-4 7005708 VWR International

Section 1 Installation and Start-up Control Panel Keys, Display, Indicators 1. Temperature Display - Displays temperature in degrees Celsius. 2. Mode Select Switch - Used to select Run, Set Temperature, Set High Alarm, Set Low Alarm, Calibrate, Backup. 3. Alarm Indicator - Light pulses on/off during an alarm condition of the cabinet. 4. Mute - Silences the audible alarm. 5. Alarm Panel - Indicates the current alarm condition. 6. Up and Down Arrows - Increases or decreases values, toggles between choices. 7. Enter - Stores the value into memory. Figure 1-9. Control Panel Keys, Display and Indicators VWR International 7005708 1-5

Section 1 Installation and Start-up Operation of the Keypad The Model 5700 Series freezer has five basic modes which allow freezer setup and operation. Press the Mode key to scroll through the mode selections. Up Arrow: Increases or toggles the parameter value. Enter: Must press Enter key to save to memory all changed values. Down Arrow: Decreases or toggles the parameter value. Mute Key: Press to silence the audible alarm. See Section 4 for alarm ringback times. Note If tipped more than 45, allow the unit to set upright for 24 hours before start up. Install Freezer To remove the freezer from the pallet, use a 7/16" wrench to remove all the bolts securing the shipping bracket to the pallet. Remove the shipping bracket. Remove the ramp boards from the pallet and place the slotted end over the ramp brackets on the pallet. The support blocks on the ramps will be facing down. Before moving the freezer, make sure the casters are unlocked and moving freely. Align the caster with the ramp boards. Use adequate personnel to roll the freezer off the pallet. The freezer can be easily pushed to the desired approved location, described in the following section. When the freezer is in position, set the front caster brakes. Note The freezer must not be moved with the product load inside. Choose Location Locate the freezer on a firm, level surface in an area with an ambient temperature between 18 C and 32 C. Provide ample room to reach the mains disconnect switch (power switch) located on the rear of the freezer. Note For proper ventilation and airflow, a minimum clearance of 5 at the rear and front and a clearance of 8 on the side of the freezer is required. Allow adequate space for lid opening. If ambient increases above 36 C, clearance at the rear of the cabinet must be increased to 8. 1-6 7005708 VWR International

Section 1 Installation and Start-up Install Wall Bumpers The parts bag, located inside the cabinet, contains the following parts. Quantity Stock # Description Purpose 2 510016 1/4-20 x 5-1/2 Bolt Wall Bumper 2 380520 Neoprene Cap Cap Protector Install the bolts into the pre-tapped holes on the back of the freezer. Install a neoprene cap on each bolt. Refer to Figures 1-3 and 1-4 for the locations of the pre-tapped holes. Remote Alarm Contacts See Figures 1-3 and 1-4 for the location of the remote alarm contacts. The remote alarm connector is located in the parts bag provided with the manual. It must be installed if connecting the freezer to an alarm system. After installing the wiring from the alarm system to the connector, install the connector to the freezer microboard and secure with the two screws provided. The remote alarm provides a NO (normally open) output, a NC normally closed) output and COM (common). The contacts will trip on a power outage, high temperature alarm or low temperature alarm. Figure 1-10 shows the remote contacts in alarm state. Figure 1-10. Remote Alarm Contacts VWR International 7005708 1-7

Section 1 Installation and Start-up Attach Power Cord Insert the power cord into the power inlet module. Place the retaining bracket (P/N 195763) over the connector. Tighten retaining screws to secure. Figure 1-11. Power Cord Retaining Bracket Connect Unit to Electrical Power Note See the serial tag on the side of the unit for electrical specifications or refer to the electrical schematics in this manual. The freezer should be operated on a dedicated grounded service. Check the voltage rating on the serial tag of the unit and compare it with the outlet voltage. Then, with the power switch turned off, plug the line cord into the wall outlet. First, turn on the freezer power switch. Then open the lower front door by grasping the bottom left corner. Locate the battery switch (Figures 1-3 and 1-4.) and turn it to Standby mode ( ). During initial freezer start-up, the system battery may require charging and the Low Battery message may appear in the message center. Note Ensure the battery switch is turned to Standby mode ( ). The rechargeable batteries require 36 hours to charge at initial start-up. A Low Battery alarm may occur until the batteries are fully charged. Should a power failure occur during the initial start-up period, the electronics will have limited operation. 1-8 7005708 VWR International

Section 1 Installation and Start-up Freezer Start-Up With the freezer properly installed and connected to power, system set points can be entered. The following set points can be entered in Settings mode: Control temperature, high temperature alarm set point, low temperature alarm set point, and (optional) BUS set point. Default settings are shown below. Control Set Point High Temperature Alarm Low temperature alarm Optional BUS Set Point -80 C -70 C -90 C -60 C Note If the set point is changed and the low temperature and high temperature alarms are set 10 from the set point, the alarm set points will be adjusted automatically to maintain a distance of at least 10 from set point. Set Operating Temperature The freezer has an operating temperature range of -50 C to -86 C, depending on ambient temperature. The freezer is shipped from the factory with a temperature set point of -80 C. To change the operating temperature set point: 1. Press the Mode key until the Set Temperature indicator lights. 2. Press the up/down arrow key until the desired temperature set point is displayed. 3. Press Enter to save the set point. 4. Press the Mode key until the Run indicator lights for Run mode If no keys are pressed, the freezer will automatically return to Run mode after 5 minutes. VWR International 7005708 1-9

Section 1 Installation and Start-up Set High Temperature Alarm The high temperature alarm activates an audible/visual warning when the freezer chamber temperature has reached or exceeded the high temperature alarm set point. To set the high temperature alarm set point: 1. Press the Mode key until the Set High Alarm indicator lights. 2. Press the up or down arrow key until the desired high temperature alarm set point is displayed. 3. Press Enter to save the setting. 4. Press the Mode key until the Run indicator lights for Run mode. If no control keys are pressed, the freezer automatically returns to Run mode after 5 minutes. Note The high alarm set point must be set at least 5 C from the control set point. Note At initial start-up, the high temperature alarm is disabled until the cabinet reaches set point, or 12 hours elapse. Set Low Temperature Alarm The low temperature alarm activates an audible/visual warning when the freezer chamber temperature has reached or decreased below the low temperature alarm set point. To set the low temperature alarm set point: 1. Press the Mode key until the Set Low Alarm indicator lights. 2. Press the up or down arrow key until the desired low temperature alarm set point is displayed. 3. Press Enter to save the setting. 4. Press the Mode key until the Run indicator lights for Run mode. If no control keys are pressed, the freezer will automatically return to Run mode after 5 minutes. Note The low alarm set point must be set at least 5 C from the control set point. 1-10 7005708 VWR International

Section 1 Installation and Start-up Run Mode Run mode is the default mode for the freezer. Run mode displays the cabinet temperature on the temperature display under normal operating conditions. In addition, the Run mode allows the high stage heat exchange temperature to be displayed. This information is scrolled by pressing the up or down arrow keys. The display returns to the operating temperature in 10 seconds if no keys are pressed. VWR International 7005708 1-11

Section 2 Calibrate Calibrate Mode Once the freezer has stabilized, the control probe may need to be calibrated. Calibration frequency is dependent on use, ambient conditions and accuracy required. A good laboratory practice would require at least an annual calibration check. On new installations, all parameters should be checked after the stabilization period. Before making any calibration or adjustments to the unit, it is imperative that all reference instruments be properly calibrated. Calibrating the Control Probe Plug a type T thermocouple reader into the receptacle located inside the lower door (see Figures 1-4 and 1-6). Compare the control temperature set point to the temperature of the measuring device. 1. Press the Mode key until the Calibrate indicator lights. 2. Press up/down arrow to match the display to calibrated instrument. 3. Press Enter to store calibration. 4. Press the Mode key to return to Run mode. Temperature Stabilization Periods Startup - Allow 12 hours for the temperature in the cabinet to stabilize before proceeding. Already Operating - Allow at least 2 hours after the display reaches set point for temperature to stabilize before proceeding. Note During calibration, the temperature display will not be available. If no keys are pressed for approximately 5 minutes while in calibration mode, the system will reset to Run mode. VWR International 7005708 2-1

Section 3 Alarms Alarms The Model 5700 Series freezer alarms are displayed on the control panel. When an alarm is active, the indicator next to the alarm description lights and an audible alarm sounds. Press the Mute key to silence the audible alarm for the ringback period. The visual alarm continues until the freezer returns to a normal condition. The alarms are momentary alarms only. When an alarm condition occurs and then returns to normal, the freezer automatically clears the alarm condition. Description Delay Ringback Relay Power Failure 1 min. 15 min. Yes High Temperature Alarm 1 min. 15 min. Yes Low Temperature Alarm 1 min. 15 min. Yes Probe Failure - see Probe Failure Alarm section 1 min. 15 min. No Door Open 1 min. 15 min. No Wrong Power 0 min. none Yes Low Battery* 1 min. 12 hours No Hot Condenser 1 min. none No High Stage Failure 0 min. 15 min. Yes All alarm delays and ringback times are ±30 seconds. * The automatic battery test runs 12 hours after initial start-up, then every 12 hours thereafter. Wrong Power The Wrong Power alarm occurs when incorrect voltage is applied to the freezer. If a 230V freezer is connected to a 120V power source or a 120V freezer is connected to a 230V power source, the electronics detect that a "Wrong Power" has been applied. Under this condition, the fans and compressors will not turn on and an audible and visual alarm occur. This alarm may also occur if the battery switch is turned on prior to applying power to the freezer. The audible and visual alarms remain until the freezer is connected to the correct power source. The audible alarm cannot be silenced under this condition. VWR International 7005708 3-1

Section 3 Alarms High Stage System Failure The "high stage system failure" condition is created when the high stage compressor and fans run for 30 minutes and are not capable of cooling the interstage heat exchanger to the proper temperature. Under this condition, the high stage compressor and fans will turn off after 30 minutes and an audible and visual alarm will occur. The audible alarm can be silenced and will ring back every 15 minutes. Probe Failure Alarm The microprocessor in Model 5700 series freezers continually scans all probes including the control probe, heat exchanger probe and condenser probe to ensure that they are operating properly. Should an error be detected, the "Probe Failure" alarm will occur as described in Section 3.1 above. If an error is detected with the control probe, the high and low stage compressors will run continuously. As a result, the cabinet temperature will decrease until it reaches the lowest temperature that the refrigeration system can maintain. If an error is detected with the heat exchanger probe, the freezer will cycle properly at its temperature set point using a 5 minute step start between the high and low stage compressors. If an error is detected with the condenser probe, there is no impact on the performance of the freezer; however, the hot condenser alarm may also occur. Contact the VWR Service department or your local distributor. Voltage Compensation Alarm In addition to the alarms listed above, another condition is detected by the controls that will result in an audible and visual alarm. If the freezer is compensating for high or low line voltage, the system will measure the compensated AC voltage. If the voltage is incorrect, the unit will stop attempting to compensate, and the compressor will run on direct line voltage. Under this condition, there will be a visual and audible alarm that can be silenced with a ringback period of fifteen minutes. This alarm condition is unlikely to occur, and as such, there is no LED alarm indicator for this condition. 3-2 7005708 VWR International

Section 4 Maintenance Clean Cabinet Exterior Wipe down the freezer exterior using soap and water and a general use laboratory disinfectant. Rinse thoroughly with clean water and dry with a soft cloth. Caution Avoid the excessive use of water around the control area due to the risk of electrical shock. Damage to the controls may also result. Clean Air Filter The air filter should be cleaned a minimum of four times per year. 1. Open the front door by grasping the handle. 2. Locate the grille on the door. See Figure 1-4. Grasp the middle of the grille material and gently pull out to remove. 3. Wash the filter material using water and a mild detergent. 4. Dry by pressing between two towels. 5. Install the filter back into the grille and close the door. Depending upon environmental conditions, the filter may need to be cleaned or replaced more frequently. If the filter becomes torn or excessively dirty, a replacement can be purchased from VWR. Order part number 760211 for 3.0 and 6.7 cu. ft. units or 760212 for 12, 17, and 20 cu. ft. units. Clean Condenser The condenser should be cleaned a minimum of once per year. 1. Open the front door by grasping the handle. See Figure 1-4. 2. Using a vacuum cleaner, exercising care to not damage the condenser fins, clean the condenser. Depending upon environmental conditions, the condenser may need to be cleaned more frequently. VWR International 7005708 4-1

Section 4 Maintenance Clean Water-cooled Condenser The water-cooled condenser can be cleaned-in-place by using the CIP procedure. Cleaning solutions can be used, depending on type of deposits or build-up to be removed. Do not use liquids that are corrosive to stainless steel or the brazing material (copper or nickel). CIP (Clean-In-Place) Procedure 1. Disconnect the unit from the water supply. 2. Drain the unit. 3. Rinse with fresh water and drain the unit again. 4. Fill with fresh water. 5. Add cleaning agent (solution and concentration dependent on deposits or build-up). 6. Circulate cleaning solution (if feasible). 7. Drain the cleaning solution. 8. Add and circulate a passivating liquid for corrosion inhibition of plate surfaces. 9. Drain this liquid. 10. Rinse with fresh water and drain. 11. Reconnect the water supply and fill the unit. 12. Return to service. Defrost Chamber 1. Remove all product and place it in another freezer. 2. Turn the unit off and disconnect it from the power source. 3. Turn off the battery switch (see figures 4-1 and 4-2). 4. Open the lid and remove sub-lids. Place towels on the chamber floor. 5. Allow the frost to melt and become loose. Remove it with a soft cloth. 4-2 7005708 VWR International

Section 4 Maintenance Defrost Chamber (continued) 7. After defrosting is complete, clean the interior with a non-chloride detergent. Rinse thoroughly with clean water and dry with a soft cloth. 8. Plug unit in and turn power switch on. 9. Turn the battery power switch to the on position. 10. Allow the freezer to operate empty overnight before reloading the product. Clean Lid Gasket The lid gasket should be cleaned a minimum of once per month. Using a soft cloth, remove any frost build-up from the gasket, sub-lids and lids. The lid gasket may need to be cleaned more frequently if dirt or excessive frost build-up prevents the door from closing properly. Replace Battery(s) The following instructions describe the battery replacement procedure for specific models. Models 5712, 5715, 5718, 5719, 5720, 5721 1. Open the front door by grasping on the handle and pulling. 2. Locate the battery power switch (Figure 4-1). Turn the battery power switch to the Off position. 3. Remove the four screws holding the recorder bezel to gain access to the battery. 4. Remove the three nuts securing the battery bracket See figure 4-1. 5. Remove the bracket and old battery. Dispose of properly. Install the new battery and secure. 6. Reconnect the battery (red to positive and black to negative). Figure 4-1. Battery and Switch location VWR International 7005708 4-3

Section 4 Maintenance 7. Replace the recorder bezel. 8. Turn on the battery power switch. 9. Close lower panel door. Models 5708, 5709, 5710, 5711 1. Open the front door by grasping the handle and pulling. 2. Locate the battery power switch (Figure 4-2). Turn the battery power switch to the off position. 3. Remove the two nuts securing the battery bracket. See Figure 4-2. 5. Remove the bracket and old battery. Dispose of properly. Install the new battery and secure. 6. Reconnect the battery (red to positive and black to negative). 7. Replace the recorder bezel. 8. Turn on the battery power switch. 9. Close lower panel door. Figure 4-2. Battery and Switch location Note For a consistent and dependable charge, replace the battery every 2 years. Replacement batteries must be rechargeable and are available from VWR. Refer to the parts list for stock number and description of the replacement batteries (part number 400159). Dispose of the used batteries in a safe manner and in accordance with good environmental practices. 4-4 7005708 VWR International

Section 4 Maintenance Prepare Unit for Storage Defrost the unit as previously described. This prepares the unit for storage. Turn off the battery power switch. Turn off the freezer power switch. If the unit has been in service, turn it off and disconnect the power cord connector before proceeding with any maintenance. VWR International 7005708 4-5

Section 4 Maintenance PREVENTIVE MAINTENANCE Freezers Your equipment has been thoroughly tested and calibrated before shipment. Regular preventive maintenance is important to keep your unit functioning properly. The operator should perform routine cleaning and maintenance on a regular basis. For maximum performance and efficiency, it is recommended that the unit be checked and calibrated periodically by a qualified service technician. The following is a condensed list of preventive maintenance requirements. See the specified section of the instruction manual for further details. We have qualified service technicians, using NIST traceable instruments, available in many areas. For more information on Preventive Maintenance or Extended Warranties, please contact the VWR Service Department. Cleaning and calibration adjustment intervals are dependent upon use, environmental conditions and accuracy required. Tips: Fill an upright by starting at the bottom near the probe and add racks to one shelf at a time. Allow freezer to recover to set point between shelves. Fill a chest by starting at the left side near the probe. Filling with room temperature racks will result in a long pull-down time. Fill unit with frozen product to help overall performance; frozen water jugs, for example. Always make certain the vacuum relief port is free of frost and ice, to allow for timely re-entry into the freezer after a door opening. Action Monthly Yearly Every 2 Years Verify ambient temperature, <90 F * Adjust door handle for firm latching, as needed Check and clean probe cover, gaskets, hinges and lid(s) of ice and snow. See Figure 1-4 for probe location. See Clean Lid Gasket. More frequent cleaning may be required, depending on use and environmental conditions. Check air filter. Clean or replace as needed. See Clean Air Filter. Check alarm back-up battery. See Connect Unit to Electrical Power in Section 1 and Replace Battery in Section 4. **Replace Check condenser fan motor for unusual motor noise or vibration. * Verify and document calibration, at the minimum, annually. See Section 2 Calibration. * Clean condenser compartment and wipe off condenser. See Clean Condenser in Section 4. * Qualified service technicians only ** Dispose of properly, according to all state and federal regulations. 4-6 7005708 VWR International

Section 5 Factory Installed Options BUS - Back Up System Note Before installation of BUS components, make sure the power to the freezer is disconnected, the battery switch is turned off and the freezer has warmed to ambient temperature. The built-in BUS (back up system) will keep the freezer chamber temperature below the critical level in the event of a power or equipment failure. If power to the freezer fails, or temperature increases to the back up alarm set point, the BUS injects liquefied gas into the chamber to keep the chamber temperature within the specified range. Install Injection Assembly The BUS operates on an internal 12-volt, rechargeable battery which is kept charged during normal operation by the integral battery charger. Figure 5-1. Mounting location 1. Locate the mounting hole for installing the injection tee assembly. See Figure 5-1. Note Cover open end of injection assembly with tape to keep insulation from entering nipple. 2. Slide 3/8 flatwasher over open end of nipple. 3. Insert covered end of injection assembly through exterior hole. 4. Remove the tape covering the end of the nipple and install the 1/8 NPT brass tee on the open end of the nipple. Place Permagum sealant between the brass tee and the interior top. VWR International 7005708 5-1

Section 5 Factory Installed Options Install Injection Assembly 5. Go to the interior and seal around the injection assembly with Permagum. 6. Install the transfer hose connecting one end to the injection assembly, the other end to the solenoid valve. Install the solenoid valve to the supply source. The solenoid mounting bracket is not required and may be discarded. Figure 5-2. Injection Assembly Note When selecting a CO2 supply cylinder, it must be equipped with a siphon tube. Install Temperature Probe 1. Plug the solenoid/probe connector into the BUS connection. Loop the probe wire back into the base/side car. Secure the connector with a screw on the ends of the connector. The connector is keyed. 2. Route the temperature probe through the probe port. The probe port is located in the upper right corner (viewed from the side) of 12.7 and 20 cu. ft. models, and in the lower right corner (viewed from the back) of 3.0 and 6.7 cu. ft. models. 3. Carefully remove the existing Permagum sealant from around the probe port opening. Figure 5-3. Probe and Solenoid Connections 5-2 7005708 VWR International

Section 5 Factory Installed Options Install Temperature Probe (continued) 4. Open the freezer lid and locate the probe cover on the upper front left wall. Remove the two Phillips head screws securing the probe cover (see Figure 5-5). 5. Route the BUS probe through the probe port, approximately 12. Secure the back-up probe to the temperature probe using a small tie wrap (Figure 5-5). Figure 5-4. Probe Access Port 6. Seal around the interior and exterior opening of the probe port with Permagum sealant. 7. Reinstall the probe cover (Figure 5-5). Figure 5-5. Probe location VWR International 7005708 5-3

Section 5 Factory Installed Options Connect Probe/Solenoid Harness 1. Carefully coil the extra probe lead in the compressor compartment, and secure it to the compartment wall with a tie wrap and tie wrap anchor provided. Additional tie wraps and anchors may be used to secure the probe lead to the exterior back wall of the freezer. 2. Loosen the terminal screws on the solenoid. Slide the spade lug connectors under the screws and tighten to secure. 3. Connect power to the freezer. Turn the freezer On, with battery switch Off (O). a. The Solenoid Engaged light on the BUS control panel will illuminate (no injection occurs). This light stays on until the unit is below BUS setpoint. b. The Low Battery indicator may also illuminate. 4. Turn the battery switch to Standby mode ( ) to charge both batteries. BUS Control Panel The following section describes the configuration and operation of the BUS. Warning When activated, this unit injects liquid nitrogen or carbon dioxide. Liquid Nitrogen can cause serious freezing (frostbite) if it comes in contact with unprotected skin or eyes. Nitrogen suppresses oxygen levels and may cause suffocation if area is not well ventilated. Refer to Appendix A for the proper handling of liquid LN2. Caution Adjust the pressure relief valve on any LN2 tank to 30 PSI maximum blow-off. Warning Carbon dioxide gas suppresses oxygen levels and may cause suffocation if area is not well ventilated. Refer to Handling Liquid CO2 in Appendix B of this manual. 5-4 7005708 VWR International

Section 5 Factory Installed Options BUS Control Panel (continued) Figure 5-6. BUS Control Panel Power - indicates the unit has AC power. Low Battery - battery charge is low. The battery needs replaced or recharged. Solenoid Engaged - BUS has opened the solenoid so it can inject gas (CO2 or LN2). Press-To-Test - Activates the solenoid and injects LN2 or CO2 into the freezer chamber as long as the button is depressed. The solenoid engaged indicator should light. If the Low Battery indicator lights during the test, replace the BUS battery. Note The solenoid will not engage if lid is open. Configure Optional BUS (Back Up System) The optional BUS can be configured for LN2 or CO2 supply. To select the supply type: 1. Press the Mode key until the Backup indicator lights. 2. Press the up or down arrow key. The display will show OP1 for CO2 selection and OP2 for LN2 selection. 3. Press Enter to save the setting. 4. Press the Mode key until the Run indicator lights for Run mode If no control keys are pressed, the freezer will automatically return to Run mode after 5 minutes. VWR International 7005708 5-5

Section 5 Factory Installed Options Set Optional BUS Set Point The optional back up system is designed to inject CO2 or LN2 into the freezer compartment if the temperature rises above back up system set point. To set the BUS set point: 1. Press the Mode key until the Settings indicator lights. 2. Press the right arrow until BACKUP = -XX is displayed in the message center. 3. Press the up or down arrow key until the desired BUS set point is displayed. 4. Press Enter to save the setting. 5. Press the Mode key until the Run indicator lights or press the right or left arrow to go to the next or previous parameter. If no control keys are pressed, the freezer will automatically return to Run mode after 5 minutes. Warning Changing the operating temperature set point can affect the BUS set point. The BUS set point will self adjust to maintain a temperature of at least 10 C above the operating temperature set point. Caution The BUS set point can not be set any colder than the high temperature alarm set point. (See Section 1 - Set High Temperature Alarm). If the back-up system is installed with CO2, then -65 C is the coldest BUS set point that can be used (if the cabinet set point is -75 C or colder). Disconnect Fitting Assembly, Transfer Hose To disconnect the freezer back-up from the gas supply: 1. Close the supply valve. 2. Depress the test button on the Back-Up System control box to remove the gas from the line. 3. Slowly disconnect the fitting assembly from the supply (in the event that any gas remains in the line). 5-6 7005708 VWR International

Section 5 Factory Installed Options Chart Recorder The following section describes the set up and operation of the optional chart recorder. Figure 5-7. Recorder Details Install Chart Paper 1. Open the plastic door of the recorder and press button #3 until the pen begins to move outward. 2. Unscrew the knob at the center of the chart and remove the paper. 3. Install the new chart paper, position the paper to the correct time line and replace the knob. 4. Remove the cap from the felt pen and press button #3. Recorder Calibration The chart recorder contains eight temperature ranges and is factoryprogrammed for the freezer. To change the recorder range: 1. Press and hold button #3 for one second, then let the pen move off the chart paper. 2. Press and hold for five seconds either button #1 or button #2. 3. Release the button and the green LED will begin to flash. Count the number of flashes to determine the present program setting. 4. To change the program setting, press the left or right arrows to increase or decrease the count. VWR International 7005708 5-7

Section 5 Factory Installed Options Recorder Calibration (continued) 5. When the desired program number is flashing, press button #3 to bring the pen arm back onto the chart. Recording will begin in the new program. Program From To 1-40 30 C 2 0 60 C 3-100 38 C 4-5 50 C 5 0 100 C 6-100 200 C 7-115 50 C 8-10 70 C Calibrate Chart Recorder The recorder must be in service for 24 hours before performing the following calibration procedure. 1. Place an accurate thermometer in the chamber next to the recorder probe. 2. Temperature probes for the recorder are located in the left front corner of the freezer chamber (Figure 1-4). 3. After about three minutes, compare the thermometer reading with the chart recorder reading. 4. If an adjustment is necessary, press the #1 button to move the pen to the left or the #2 to move the pen to the right. The button must be held about five seconds before the pen begins to move. Release the button when the pen position matches the thermometer. Note The felt-tip pen on the recorder requires periodic replacement. Usually the ink will appear to fade before replacement becomes necessary. Additional pen tips may be purchased from VWR. Datalogger Dataloggers and ELPRO evaluation software provide monitoring and documentation of temperature and alarm conditions. The dataloggers have a memory capacity of 64,000 measured values or data points. Temperature is measured, stored and displayed. Alarm conditions are recorded. Evaluation software permits data to be downloaded to a PC. A variety of statistical information is provided through calculations, analysis, graphs and printed reports. Refer to the ELPRO documentation for operating instructions for the datalogger. 5-8 7005708 VWR International

Section 5 Factory Installed Options Water-cooled Condenser The water-cooled condenser (P/N 195145, 195611) is a factory installed option and requires a qualified technician at freezer installation. The installation should include proper adjustment of the regulating valve, which controls the discharge pressure. Specifications for this option are displayed in Table 5-1. Water Source Tower City Water Pressure Water Temperature Range Inlet Connection Outlet Connection Not to exceed 150 psig Not to exceed 29.4C (85F) 0.5 compression 0.5 compression Flow Rate Required 3.0 gallons (11.4 liters) per minute 1.0 gallon (3.8 liters) per minute Drain Required No (return line is required) Yes Table 5-1. Water Cooled Condenser Specifications VWR International 7005708 5-9

Section 6 Specifications Model 5708 5709 Temperature Range Exterior Dimensions Interior Dimensions Capacity Refrigeration Insulation -50 C(-58 F) to -86 C(-123 F) in an 18C to 32C * (64.4F to 89.6F) ambient 28.5 W x 43.8 H x 28.8 F-B (72.4cm x 111.3cm x 73.2cm) Add 7.9 (20.1cm) to F-B for wall spacer/handle. 18.5 W x 16.5 H x 18.5 F-B (47.0cm x 41.9cm x 47.0cm) 3.0 cu. ft. (84.9 liters) Cascade system, (2) hermetically-sealed compressors Non CFC, foamed-in-place urethane: 5.0" (12.7cm) cabinet; 2.0" (5.0cm) lid; 1.0" (12.5cm) sub-lids Electrical Breaker Requirements Shipping Weight 120VAC, 1 PH, 60 Hz, 10.5 FLA Operating Range: 108VAC-130VAC 15 Amp, 120VAC, Dedicated Circuit, 15 Amp Time Delay Breaker 432 lbs. ( 196.0 kg) 230VAC, 1 PH, 50 Hz, 5.4 FLA Operating Range: 208VAC-240VAC 15 Amp, 230VAC, Dedicated Circuit, 15 Amp Time Delay Breaker Model 5710 5711 Temperature Range Exterior Dimensions Interior Dimensions Capacity Refrigeration Insulation Electrical -50 C(-58 F) to -86 C(-123 F) in an 18C to 32C * (64.4F to 89.6F) ambient 48.0 W x 43.8 H x 28.8 F-B (121.9cm x 111.3cm x 73.2cm) Add 7.9 (20.1cm) to F-B for wall spacer/handle. 38.0 W x 16.5 H x 18.5 F-B (96.5cm x 41.9cm x 47.6cm) 6.7 cu. ft. (189.7 liters) Cascade system, (2) hermetically-sealed compressors Non CFC, foamed-in-place urethane: 5.0" (12.7cm) cabinet; 2.0" (5.0cm) lid; 1.0" (12.5cm) sub-lids 120VAC, 1 PH, 60 Hz, 10.5 FLA Operating Range: 108VAC-130VAC 230VAC, 1 PH, 50 Hz, 5.4 FLA Operating Range: 208VAC-240VAC Breaker Requirements Shipping Weight 15 Amp, 120VAC, Dedicated Circuit, 15 Amp Time Delay Breaker 566 lbs. ( 256.7 kg) 15 Amp, 230VAC, Dedicated Circuit, 15 Amp Time Delay Breaker VWR International 7005708 6-1

Section 6 Specifications Model 5715 5712 Temperature Range Exterior Dimensions Interior Dimensions Capacity Refrigeration Insulation Electrical -50 C(-58 F) to -86 C(-123 F) in an 18C to 32C * (64.4F to 89.6F) ambient 72.0 W x 40.5 H x 28.8 F-B (182.9cm x 102.9cm x 73.2cm) Add 7.9 (20.1cm) to F-B for wall spacer/handle. 42.5 W x 28.0 H x 18.5 F-B (108.0cm x 71.1cm x 47.6cm) 12.7 cu. ft. (360 liters) Cascade system, (2) hermetically-sealed compressors Non CFC, foamed-in-place urethane: 5.0" (12.7cm) cabinet; 2.0" (5.0cm) lid; 1.0" (12.5cm) sub-lids 120VAC, 1 PH, 60 Hz, 16.0 FLA Operating Range: 108VAC-130VAC 230VAC, 1 PH, 50/60 Hz, 12.0 FLA Operating Range: 208VAC-240VAC Breaker Requirements Shipping Weight 20 Amp, 120VAC, Dedicated Circuit, 20 Amp Time Delay Breaker 716 lbs. (324.8 kg) 15 Amp, 230VAC, Dedicated Circuit, 15 Amp Time Delay Breaker Model 5718 5719 Temperature Range Exterior Dimensions Interior Dimensions Capacity Refrigeration Insulation -50 C(-58 F) to -86 C(-123 F) in an 18C to 32C * (64.4F to 89.6F) ambient 87.6.0 W x 40.5 H x 28.8 F-B (222.5cm x 102.9cm x 73.2cm) Add 7.9 (20.1cm) to F-B for wall spacer/handle. 58.8 W x 28.0 H x 18.5 F-B (149.4cm x 71.1cm x 47.0cm) 17.0 cu. ft. (481.4 liters) Cascade system, (2) hermetically-sealed compressors Non CFC, foamed-in-place urethane: 5.0" (12.7cm) cabinet; 2.0" (5.0cm) lid; 1.0" (12.5cm) sub-lids Electrical Breaker Requirements Shipping Weight 120VAC, 1 PH, 60 Hz, 16.0 FLA Operating Range: 108VAC-130VAC 20 Amp, 120VAC, Dedicated Circuit, 20 Amp Time Delay Breaker 821 lbs. ( 372.4 kg) 230VAC, 1 PH, 50/60 Hz, 12.0 FLA Operating Range: 208VAC-240VAC 15 Amp, 230VAC, Dedicated Circuit, 15 Amp Time Delay Breaker 6-2 7005708 VWR International

Section 6 Specifications Model 5721 5720 Temperature Range -50 C(-58 F) to -86 C(-123 F) in an 18C to 32C * (64.4F to 89.6F) ambient Exterior Dimensions Interior Dimensions Capacity Refrigeration Insulation 96.0 W x 40.5 H x 28.8 F-B (243.8cm x 102.9cm x 73.2cm) Add 7.9 (20.1cm) to F-B for wall spacer/handle. 66.5 W x 28.0 H x 18.5 F-B (168.9cm x 71.1cm x 47.6cm) 20.0 cu. ft. (566.3 liters) Cascade system, (2) hermetically-sealed compressors Non CFC, foamed-in-place urethane: 5.0" (12.7cm) cabinet; 2.0" (5.0cm) lid; 1.0" (12.5cm) sub-lids Electrical Breaker Requirements Shipping Weight 120VAC, 1 PH, 60 Hz, 16.0 FLA Operating Range: 108VAC-130VAC 20 Amp, 120VAC, Dedicated Circuit, 20 Amp Time Delay Breaker 833 lbs. ( 377.8 kg) 230VAC, 1 PH, 50/60 Hz, 12.0 FLA Operating Range: 208VAC-240VAC 15 Amp, 230VAC, Dedicated Circuit, 15 Amp Time Delay Breaker Certifications Declaration of Conformity is available from the factory Safety Specifications Indoor Use Only Altitude - up to 2,000 meters Temperature - 5 C to 43 C Humidity - Maximum RH 80% for temperatures up to 31 C, decreasing linearly to 50% RH at 40 C Mains Supply Fluctuations - Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage Installation Category II 1 Pollution Degree 2 2 Class of Equipment I 1 Installation category (overvoltage category) defines the level of transient overvoltage which the instrument is designed to withstand safely. It depends on the nature of the electricity supply and its overvoltage protection means. For example, in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories, the expected transient overvoltage is 2500V for a 230V supply and 1500V for a 120V supply. 2 Pollution degree describes the amount of conductive pollution present in the operating environment. Pollution degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation. VWR International 7005708 6-3

Section 7 Parts VWR International 7005708 7-1

Section 7 Parts 7-2 7005708 VWR International

Section 7 Parts VWR International 7005708 7-3

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Section 7 Parts VWR International 7005708 7-9

Section 7 Parts 12.7, 17, 20 cu. ft Chest Freezer Sidecar 5612,5615,5618,5619, 5620,5621,5712,5715 5718,5719,5720,5721 5615-200-2-B Rev. 1 Page1of2 7-10 7005708 VWR International

Section 7 Parts AKS 12.7, 17, 20 cu. ft Chest Freezer Sidecar 5612,5615,5618,5619, 5620,5621,5712,5715 5718,5719,5720,5721 5615-200-2-B Rev. 1 Page2of2 VWR International 7005708 7-11

Section 7 Parts 7-12 7005708 VWR International

Section 7 Parts VWR International 7005708 7-13

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Section 8 Refrigeration Schematics VWR International 7005708 8-1

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Section 8 Refrigeration Schematics VWR International 7005708 8-3

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Section 8 Refrigeration Schematics VWR International 7005708 8-5

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Section 9 Electrical Schematics VWR International 7005708 9-1

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Section 9 Electrical Schematics VWR International 7005708 9-12

Section 10 Warranty Information VWR SCIENTIFIC PRODUCTS STANDARD AND SIGNATURE SERIES ULT FREEZER WARRANTY - USA The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the warranty period. During the first two years of the warranty period, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at VWR/Thermo Electron Corporation s expense, labor included. The Signature Series ULT Freezers include an additional three year warranty on the compressors, parts only, F.O.B. factory. Installation and calibration is not covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior to any work being performed. Expendable items, i.e., glass, filters, pilot lights, light bulbs and door gaskets are excluded from this warranty. Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the equipment or to the component part beyond the original two year warranty period. VWR and/or Thermo must give prior approval for the return of any components or equipment. THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products. Your local VWR Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction manuals carefully detail equipment installation, operation, and preventive maintenance. If equipment service is required, please call the Technical Services Office at 1-888-213-1790 (USA and Canada) or 1-740-373-4763. We're ready to answer your questions on equipment warranty, operation, maintenance, service, and special applications. Outside the USA, contact your local distributor for warranty information. ISO 9001 REGISTERED Rev. 4 5/8/03 VWR International 7005708 10-1

Section 11 Warranty Information VWR SCIENTIFIC PRODUCTS STANDARD AND SIGNATURE SERIES ULT FREEZER WARRANTY - INTERNATIONAL The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows for shipping time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the warranty period. During the first two years of the warranty period, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at VWR/Thermo Electron Corporation's expense, labor excluded. The Signature Series ULT Freezers include an additional three year warranty on the compressors, parts only, F.O.B. factory. Installation and calibration is not covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior to any work being performed. Expendable items, i.e., glass, filters, pilot lights, light bulbs and door gaskets are excluded from this warranty. Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the equipment or to the component part beyond the original two year warranty period. VWR and/or Thermo must give prior approval for the return of any components or equipment. THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products. Your local VWR Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction manuals carefully detail equipment installation, operation, and preventive maintenance. If equipment service is required, please call your local distributor or the Technical Services Department at 1-740-373-4763 (1-888-213-1790 in USA or Canada). We're ready to answer your questions on equipment warranty, operation, maintenance, service, and special applications. Outside the USA, contact your local distributor for warranty information. ISO 9001 REGISTERED Rev. 5 5/8/03 10-2 7005708 VWR International

Appendix A Handling Liquid Nitrogen Warning Contact of liquid nitrogen or cold gas with the skin or eyes may cause serious freezing (frostbite) injury. Handle liquid nitrogen carefully. The extremely low temperature can freeze human flesh very rapidly. When spilled on a surface the liquid tends to cover it completely and intimately, cooling a large area. The gas issuing from the liquid is also extremely cold. Delicate tissue, such as that of the eyes, can be damaged by an exposure to the cold gas which would be too brief to affect the skin of the hands or face. Never allow any unprotected part of your body to touch objects cooled by liquid nitrogen. Such objects may stick fast to the skin and tear the flesh when you attempt to free yourself. Use tongs to withdraw objects immersed in the liquid, and handle the object carefully. Wear protective clothing. Protect your eyes with a face shield or safety goggles (safety glasses without side shields do not give adequate protection). Always wear gloves when handling anything that is, or may have been, in immediate contact with liquid nitrogen. Insulated gloves are recommended, but heavy leather gloves may also be used. The gloves should fit loosely, so that they can be thrown off quickly if liquid should splash into them. When handling liquid in open containers, it is advisable to wear high-top shoes. Trousers (which should be cuffless if possible) should be worn outside the shoes. VWR International 7005708 A -1