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GENERAL - BEST PRACTICE CLEANING EQUIPMENT Sweep or rinse away loose debris. On floors, use a dust-attracting mop, preferably not a broom Where possible, make use of vacuum (Clean bag daily) Remove spills (feaces, urine, blood, vomitus, pus) with disposable paper towel and spray area with F10CL prior to mopping. Use mops on floor surfaces only and brushware with PVC backing and nylon bristles in cages Use a two-bucket system and two sets of cleaning equipment (one in use, one stored clean and dry) Mops / Brushes / Cloths - Rinse with water - Wash thoroughly using - Dry out excess water Walls and Tiles - Wash from top to bottom using - Wash off visible dirt (e.g. blood) using Counters / Bench Tops Chairs Cupboards and Shelving Waste Bins - Remove plastic liner, tie closed and discard - Trigger spray inside of new plastic liner as well as top Door handles / Light switches / Telephones / Keyboards - Trigger spray using F10CL onto a paper towel or clean cloth and wipe down Benches and sinks / Wash basins - Wash with water to remove debris - Wash/Scrub with nylon brush using Floors - Sweep clear with dust-attracting mop, preferably not a not broom. If possible, vacuum - Avoid dispersal of dust and bacteria by keeping mop head in contact with floor and not lifting at end of each stroke. - Apply to floor using clean mop and wash with long, uninterrupted strokes. - Discard used water from mop into separate bucket (use two compartment system) - Apply F919SC, scrub lightly with nylon broom, rinse and mop dry Floor drains / Sink wastes - Remove drain covers/traps and scrub with F919SC - Flush with large volumes of clean water - Pour 1 litre of diluted F1CL down drain Carpets - Vacuum, do not brush or sweep. Check inner paper bag and clean out if more than half full. Clean out away from patient areas with minimum dispersal of dust. Toilets and sluices - Scrub inside of bowl with nylon brush on long handle using - Trigger spray seat, cover and handle using F10CL and wipe down with paper towel RECEPTION AREA S Scale CONSULTING ROOMS Examination table and mattress - Wash visible debris off with clean water When necessary End of Shift End of Shift Hang to dry Monthly When necessary Twice daily /when visibly dirty /when soiled Monthly 5 min

Application Frequency Dilution per (top and underneath) Trolleys Thermometers - Use two sets, one in use and one stored dry -, wipe with disposable paper - Wipe using F10 Wipes or spray with F10CL and wipe with paper towel, THEATRES / PROCEDURE ROOMS Use separate set of cleaning equipment, clean and disinfect as recommended above Operating table - Wash visible debris off with clean water (top and underneath) Linen (Drapes) - Soak soiled/bloodstained linen in cold water before sterilization - Septic cases: Soak in before washing and sterilization EQUIPMENT / INSTRUMENTS Anaesthetic Machines, Suction Apparatus, Ventilators and Humidifiers - Wash bottles and tubing using and hang to dry thoroughly - Rinse bottles and tubing using F10CL - Wipe apparatus down using F10CL Incubators, Fridges, Microwave Ovens, O/h Lights, Defibrillators and Hydro baths Endoscopes - Follow manufacturer s instructions with regards to rinsing after use as well as use of enzymatic agent. - Rinse for 1 minute. Wash using for utes if mechanical (5 minutes if ultrasonic), rinse for 1 minute. - Soak in F10 STERILANT(RTU) for utes, rinse with sterile water or use without rinsing - OR soak in F10CL (1:20) for utes, rinse with sterile water or use without rinsing NOTE: If a gluteraldehyde had been used to disinfect the instrument, it must be thoroughly washed and wiped with alcohol before immersed in F10. A reddish residue may form on the instrument as the result of an interaction between the aldehyde and F10. This will not damage the instrument and can be avoided by thorough cleansing to remove all aldehyde residues. DO NOT USE F10 STERILANT ON ALUMINIUM Electric razors and blades - Scrub with nylon brush using - Trigger spray using F10CL - Apply blade wash/lubricant - Store dry Endotrachael Tubes - Wash thoroughly with (rinse lumen thoroughly to clear away mucous) - Soak in F10CL - OR soak in F10 STERILANT (RTU) - Rinse with sterile water OR remove without rinsing - Store clean and dry Needles, Syringes, I/V Catheters, Drip Sets, Suture Material, Nail Brushes - Single use is safest and best practice! - Do not store in disinfectant - Wash using After every case 30 min / Hang to dry / / 1 min 30 min Page 2 of 6

- Soak in F10 STERILANT (RTU) - Rinse with sterile water OR remove without rinsing - Store clean and dry Instruments - Cover soiled instruments with sterile damp swab to avoid blood drying on them - Wash thoroughly in lukewarm water and using a soft nylon brush. Rinse. - Dry thoroughly and lubricate with instrument lubrication. - Sterilize through moist steam or cold sterilization process Cold Sterilization - Fully immerse clean and dry instruments in fresh solution of F10 STERILANT (RTU) - Remove using sterile instrument or wearing sterile gloves (rinse with sterile water or use without rinsing) - Use immediately or store in sterile container NOTE: If a gluteraldehyde had been used to disinfect the instrument, it must be thoroughly washed and wiped with alcohol before immersed in F10. A reddish residue may form on the instrument as the result of an interaction between the aldehyde and F10. This will not damage the instrument and can be avoided by thorough cleansing to remove all aldehyde residues. DO NOT USE F10 STERILANT ON ALUMINIUM Patient Skin preparation - Clip wide area neatly and as short as possible using clean no 40 blade - Wash area thoroughly using F10 HAND SCRUB and lukewarm water, rinse, dab away excess moisture. Repeat until no visible dirt left on skin - Spray area with F10 SKIN PREP (RTU) and wipe with clean swab. Repeat - Move patient onto operating table, spray surgical site with F10 SKIN PREP (RTU) and leave to dry HANDWASHING Sterile and aseptic invasive s - Wash hands with F10 HAND SCRUB and lukewarm water while keeping hands higher than elbows and using friction for 2 minutes; close tap with elbows - Dry hands with sterile towel from fingers to elbow General Practice - Wash hands with F10 HAND SCRUB and water using friction for 30 seconds - Dry hands with paper towel; use paper towel to close tap - Use F10 HAND GEL as an alternative for decontamination of hands between cases X-RAY EQUIPMENT Lead gloves and gowns - Wash using if visibly soiled FOOD / DRINK BOWLS, FEEDING BOTTLES, FOOD MIXERS - Rinse well in hot water - Wash using hot water and - Immerse in F10CL or trigger spray LAUNDRY AND BEDDING - Remove organic matter - Soak in - Wash in washing machine (65 C for OR 71 C for 3 minutes) - Tumble or sun dry - To eliminate persistent odors, add app of F10 Odor Eliminator to rinse water During After After cleaning and when required Between cases/ after contamination/ after any body function If visibly soiled 4ml () 4ml () 1ml () 30 sec 2 min 30 sec 30 min Page 3 of 6

CAGES / KENNELS / SHOWER RACKS - Remove loose debris and wash down with water - Scrub using nylon brush / broom and - Apply F10CL - Deep clean using F919SC and brush off with water INSECT CONTROL - Keep all foodstuffs covered and out of sunlight refrigerate when not in use Lightly spray walls and ceilings in areas where fly control is required with F10 INSECTICIDE SURFACE SPRAY, repeat 3 or 4 applications to build-up residual coating, thereafter spray when required FOGGING - Disinfect air spaces and hard surfaces after cleaning using F10CL through an electrical fogging machine - Or by means of a garden sprayer - Or by using a total evacuation F10 Disinfectant Aerosol Fogger canister AIRCON SYSTEMS - Remove filters, wash using F919SC, rinse with water - Immerse filter in F10CL - F10CL can also be sprayed / fogged into air ducting SOAP DISHES / SOAP DISPENSERS - Use of soap dishes not recommended. Wash and dry daily if used. - Fill dispensers half way, do not top up - When bottle is empty, wash using hot water and - Trigger spray nozzles using F10CL / Between patients After cleaning /monthly When necessary /Terminal Disinfection Monthly Monthly Monthly filling For full information on the above mentioned products please refer to the product information brochures. - F10CL, and F919SC can be applied by hand / hand spray / HP Washer - F10CL may also be applied through a fogging machine These user guidelines are based on the Infection Control Procedures in use at the Onderstepoort Veterinary Academic Hospital since 1996. These were drawn up by Sr. Linda Muller (OVAH Matron 1994-2003) and Mr John Temperley of Health and Hygiene (Pty) Ltd. and form the basis of the SAVC s Guidelines for Disinfection and Antiseptics in Veterinary Practice. Page 4 of 6

CL ISOLATION FACILITIES CLEANING EQUIPMENT Use dedicated set of cleaning equipment for Isolation facility ONLY Use a dust-attracting mop, preferably not a broom Where possible, make use of vacuum (Clean bag daily) Remove spills (feaces, urine, blood, vomitus, pus) with disposable paper towel and spray area with F10CL prior to mopping Use mops on floor surfaces only and brushware with PVC backing and nylon bristles in cages Use a two-bucket system and two sets of cleaning equipment (one in use, one stored clean and dry) Always start shift with CLEAN and DRY equipment Mops / Brushes / Cloths - Rinse with water - Wash thoroughly using - Dry out excess water GENERAL Walls and Tiles - Wash from top to bottom using - Wash off visible dirt (e.g. blood) using Work surfaces Cupboards and Shelving Waste Bins - Remove plastic liner, tie closed and discard - Trigger spray inside of new plastic liner as well as top Door handles / Light switches / Telephones / Keyboards - Trigger spray using F10CL onto a paper towel or clean cloth and wipe down Wash basins and Sinks - Wash with water to remove debris - Wash/Scrub with nylon brush using - Rinse with water Floors - Sweep clear with dust-attracting mop, preferably not a broom. If possible, vacuum - Avoid dispersal of dust and bacteria by keeping mop head in contact with floor and not lifting at end of each stroke. - Apply to floor using clean mop and wash with long, uninterrupted strokes. - Discard used water from mop into separate bucket (use two compartment system) - Apply F919SC in warm water, scrub lightly, rinse and mop dry Floor drains - Remove drain covers/traps and scrub with F919SC - Flush with large volumes of clean water - Pour 1 litre of diluted F10CL around and down drain Toilets and sluices - Scrub inside of bowl with nylon brush on long handle using - Trigger spray seat, cover and handle using F10CL and wipe down with paper towel Scale Examination table and mattress - Wash visible debris off with clean water (top and underneath) End of shift End of shift Monthly/ when necessary Twice daily /When visibly dirty /When soiled / Monthly 5 min Hang to dry 5 min 3 min 3 min Page 5 of 6

Trolleys Thermometers - Use two sets, one in use and one stored dry -, wipe with disposable paper - Wipe using F10 Wipes or spray with F10CL and wipe with paper towel Incubators, Fridges, Microwave Ovens, O/h Lights, Defibrillators Electric clippers and blades - Scrub with nylon brush using - Trigger spray using F10CL - Apply blade wash/lubricant Needles, Syringes, I/V Catheters, Drip Sets, Suture Material, Nail Brushes - Single use is safest and best practice! - Do not store in disinfectant - Wash using - Soak in F10CL - Rinse with sterile water HANDWASHING - Wash hands with F10 Hand Scrub and lukewarm water using friction for 2 minutes - Dry hands with paper towel; use paper towel to close tap Use F10 Hand Gel as an alternative for decontamination of hands between cases FOOD / DRINK BOWLS - Use dedicated food and drink bowls - Rinse well in hot water - Wash using hot water and - Immerse in F10CL or trigger spray - Dry hands with paper towel; use paper towel to close tap LAUNDRY AND BEDDING - Use dedicated blankets / towels - Remove organic matter - Soak in - Wash in washing machine (65 C for OR 71 C for 3 minutes) - Tumble or sun dry CAGES / KENNELS / SHOWER RACKS - Remove loose debris and wash down with water - Scrub using nylon brush / broom and - Apply F10CL - Deep clean using F919SC and brush off with water FOGGING Disinfect air spaces and hard surfaces after cleaning with F10CL through an electrical fogging machine or by means of a garden sprayer or by using a total evacuation F10 Disinfectant Fogger canister. AIRCON SYSTEMS - Remove filters, wash using F919SC, rinse with water - Immerse filter in F10CL - F10CL can also be sprayed / fogged into air ducting SOAP DISHES / SOAP DISPENSERS - Use of soap dishes not recommended. Wash and dry daily if used. - Fill dispensers half way, do not top up - When bottle is empty, wash using hot water and - Trigger spray nozzles using F10CL FOOT BATHS - Scrub out using - Fill with water and F10CL to cover at least 15 cm For full information on the above mentioned products please refer to the product information brochures. - F10CL, and F919SC can be applied by hand / hand spray / HP Washer - F10CL may also be applied through a fogging machine These user guidelines are based on the Infection Control Procedures in use at the Onderstepoort Veterinary Academic Hospital since 1996. These were drawn up by Sr. Linda Muller (OVAH Matron 1994-2003) and Mr John Temperley of Health and Hygiene (Pty) Ltd. and form the basis of the SAVC s Guidelines for Disinfection and Antiseptics in Veterinary Practice. Visibly soiled/ Between cases/ after contamination leaving unit 4ml (as is) 1ml (as is) 30 min 2 min 15 min Between patients /monthly Monthly Monthly Monthly filling/ /When necessary 2-3 min Page 6 of 6