ChBE Safety Training July 2016 Emily Edwards Lab Coordinator Chemical & Biological Engineering Tufts University 4 Colby St., Room 151 617-627-2172
Required Training Chemical Safety Choose 1 of 3 Options A chemical safety training certificate is required before admittance to any laboratory Option 1 30-minute video + written test Must get 80% to pass Certificate expires after one year Email me a copy of your certificate I need proof of training for the annual lab inspections Option 2 Classroom training from BME Lab Coordinator No set schedule, just when demand is high Building-specific information No certificate send me an email so I can add a note to my files Option 3 - Preferred Classroom training from EH&S, usually at 200 Boston General information like Option 1 See website for scheduled times and registration Email me a copy of your certificate
Required Training Bio Safety Classroom Training Required: Classroom training if working with bio materials Classroom training from EH&S, usually at 200 Boston General information about bio safety + OSHA Bloodborne Pathogen Standard See website for scheduled times and registration Email me a copy of your certificate Combined: Classroom training for chemical & bio materials Option 3 above + bio training to the left See website for scheduled times and registration Email me a copy of your certificates
Personal Protective Equipment (PPE) Tufts EH&S does random unannounced inspections looking for people not wearing PPE Write your name on your coat Latex or Nitrile? Check chemical compatibility before choosing Put your name or initials on them with a sticker Face shield and thermal gloves are required when dispensing LN Hearing protection and sign on door are required when using a sonicator (dismembrator)
Lab Regulations No open-toed shoes allowed in the lab Keep a pair of shoes at your desk, if needed Remove one glove before touching a door knob or anything outside the lab New Regulation: Bunsen burner tubing must be a special cloth-covered steel tubing
Lab Regulations No backpacks or jackets allowed in the lab Request a locker if needed No food or drinks including water bottles Leave outside the lab
Safety Warning Requirements Sign on the door reflects hazards inside + contact information for after-hours emergencies, must be revised when needed The number in each diamond reflects the level of danger: 0 through 4 Read the SDS for your chemicals before using
Lab Sink Water Testing All lab sinks drain to the Machine Shop Giant ph neutralizing system and holding tank for periodic testing Fines for too much metal in the waste water recent fine for mercury, not sure what else they test for All chemicals must go into waste containers, never down the sink
Labels No Mystery Substances Label your containers and samples: No mystery liquids or solids are allowed in any lab. This includes containers of water and reactor vessels Use large labels for stock solutions and reactor vessels Use small labels for vials or small flasks
Emergency Procedures Safety Features inside the lab Calling for help: Tufts Emergency Number 617-627-6911 (or 911) Program this into your phone 6-6911 from a campus phone Determining a true emergency: Fire Non-minor injuries or burns Very large chemical spills Very large gas leaks Anything involving especially dangerous chemicals or gases Chemical fume hood failure if hood contains dangerous chemicals or gases
Emergency Procedures Spill Kit Procedure: Wear PPE Use absorbent material inside the bucket to soak up the liquid Place into bag Fill out waste tag and attach it to the bag Place the bag in your Pickup SAA Inform your PI and request a refill from the Lab Coordinator Minor Injuries: Use 1 st Aid Kit See Lab Coordinator Go to Tufts Student Health Services After-hours Event: Fire alarm, chemical smell, etc. Call from Tufts Police to the emergency contact number on file or on door
Chemical Storage Locations Flammables Cabinet or Special Fridge Alcohols Solvents Alcohol-fueled Bunsen burners Special flame-proof fridge Inside a Fume Hood Especially toxic chemicals Disrupts functionality and safety of hood must be minimal Corrosives Cabinet Separate organic & inorganic acids Separate acids & bases
Biomaterial Storage Locations Ultra-cold Freezer -80 C (-112 F) For long-term cell storage Frost builds up around door and prevents door from closing must scrape off to maintain temperature Fridge & Freezer 4 C & -20 C, check storage instructions carefully No auto-defrosting freezer they cycle above freezing periodically to melt the frost Freezers must be defrosted Must have stickers that say no food and no flammables
Compressed Gas Cylinders Clamps mount to table or wall Chain or strap Allowed 3 cylinders on 1 clamp Top of tank is ceramic and could break if dropped (see Myth Busters episode) Remove regulator and screw cap on tightly before moving Cylinders are safe when cap is on verified this with our Airgas delivery person
Compressed Gas Cylinders Types of gases are color-coded: flammable, oxidizer, nonflammable Each individual gas has a different nozzle thread to avoid mixing of incompatible gases inside the regulator; for example, CGA 320 is for CO 2 Choose the proper PSI range when purchasing regulator Flammable Gases have special rules Allowed only 1500 ft 3 (STP) per section of our building, large cylinders have ~285 ft 3 = 6 large cylinders. We need more than 6 per section so all flammables must now be size 80 cylinders. Tubing must be stainless steel Flashback arrestor is required: fuel for flammable gases and oxygen for oxidizers Gas sniffers are required for any dangerous gas (H 2, H 2 S, etc.)
Chemical Disposal Do not pour chemicals down the drain periodically inspected Liquids Separate Containers Acids Bases + Misc. Alcohols, solvents Solids: Sharps Tips Broken glass Empty glass bottles Solids: Non-sharp Powder, goop Chemically-soaked paper towels Weigh boats Empty plastic bottles Pipets in sleeves New Procedure! Put pipet in sleeve and place in nonsharps bucket
Chemical Disposal Tags: Must be on all non-bio containers Immediately fill in: Lab # PI As you fill the container, the following must be accurate at all times: Chemicals % (best guess) Hazards Leave dates blank until picked up SAAs New Procedure! Active SAA Pickup SAA + Add new waste here Place full containers here Keep the bucket new bag inside Trash the sharps bin get new one from Lab Coordinator Weekly lab pickup by waste management company
Liquids: Add bleach until v/v % is ~10% wait at least 30 minutes pour down drain Bio-material Disposal Solids: Sharps Tips Broken glass Syringe needles Blades New Procedure! Put pipet in sleeve and place in nonsharps bucket Solids: Non-sharp Gloves Bio-soaked paper towels Cuvettes Pipets in sleeves Bag must be autoclave-able
Bio-material Disposal New Procedure Secure lid to bin with tie wraps before leaving your lab Autoclave Sterilize glassware, media, lab supplies or non-sharp bio waste Training required before using Must sign up for a timeslot before using Separate training for sterilizing bio waste involving a logbook and tags Cages for Sharps Bins Cages must remain locked Full bins must be logged in logbook Picked up by Stericyle every 2 weeks
Bio vs. Chemical Disposal What if you have an item that contains both bio and chemical material? Bio-related chemicals: Salt Buffer Media Autoclave No chemicals are allowed in the autoclave! Other autoclave hazards Non-sharps bio waste in autoclave-able bag Chemicals that would destroy cells: Solvents Inorganic compounds Alcohols All items must be in autoclave-able tub when inside the autoclave
Chemical Fume Hood vs. Bio Cabinet Fume Hood Pulls air in from the room and vents it outside to protect person from fumes Air flow cannot be turned off Sash height and obstructions can greatly affect your safety Bio Cabinet (aka Laminar Flow Hood) Pulls in air from the room, filters and recirculates to prevent bio material from escaping into the room Air flow is turned on and off by the user Inside surfaces must be decontaminated
Fume Hood Safety Proper use of chemical fume hood: Hoods are calibrated to be safe for use with the sash below designated height shown on the sticker (usually 18 ) Keep sash as low as possible without hindering your work below your shoulders to avoid breathing fumes Close the sash when not in use to contain the chemical fumes
Facilities Problems Contact Lab Coordinator: DI water, autoclave, vacuum Low flow in fume hood (containing nonhazardous chemicals or gas) Chemical smell Broken equipment Safety supplies Any random question Fill out a Facilities Work Request: Dripping faucet Electrical problems Burned out lights Restroom problems
Lab Inspections Annual Lab Inspection: General lab safety Fill out form in advance and fix issues Inspected by EH&S Receive report with findings fix and reply Semi-Annual Chemical Audit: Search for especially hazardous or explosive chemicals Also look for storage issues acids & bases not separated organic & inorganic acids Receive a report with findings fix and reply Annual Testing: Flowrate of chemical fume hoods & bio cabinets Eyewash & showers
Eco-Friendly Practices Try to avoid disposing of disposables in order to reduce our dependence on oil: Try to minimize your use of weigh boats, jars & vials Re-use pipets & tips if using DI water or alcohols You must dispose of anything that came into contact with live cells
Eco-Friendly Practices Chemical fume hoods consume a large amount of energy Tufts is working hard to reduce the amount of energy consumed in our building Close the sash when not in use!
Eco-Friendly Practices Paper, rigid plastic, glass & metal are banned from landfills in MA See the signs at the recycling stations for more information
Eco-Friendly Practices Which is more environmentally friendly? Paper towels vs. Kimwipes Must consider the energy used for manufacturing and transportation
This presentation will be available on the ChBE website under the Graduate Program tab Department Website