Arnold Schumann, Kevin Hostler, Laura Waldo, Wije Bandaranayake (UF/IFAS, CREC) 2015 Fluid Forum February 17, 2015 Scottsdale, AZ

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Advanced Production Systems for Florida Citrus and Blueberry Crops Arnold Schumann, Kevin Hostler, Laura Waldo, Wije Bandaranayake (UF/IFAS, CREC) 2015 Fluid Forum February 17, 2015 Scottsdale, AZ

Introduction During the past 5 years new citrus production systems in Florida were evaluated to develop strategies against endemic HLB disease Advanced fertigation (open hydroponics), rootstocks, and higher planting densities were key components used to improve grove performance Based on the initial successes with citrus, we expanded the research into a new citrus fertigation x rootstock x planting density experiment in 2012, and a pilot fertigation experiment with highbush blueberries in 2013 Updates for both trials will be provided here

The situation in HLB-endemic Florida By year 5 most young groves are already seriously compromised by HLB Economic break-even in conventionally spaced processed orange groves is about 10-12 years The risk of not reaching economic break-even is high Advanced fertigation (open hydroponics), rootstocks, and higher planting densities are tools that can accelerate growth and early fruit production, hastening the achievement of economic break-even

HLB-affected Hamlin yield in year 5:

Advanced Citrus Production Systems (ACPS) High density (363 trees/acre) precocious scion/rootstock ( Hamlin /C35) + open hydroponics (N,P,K, Mo) = 622 boxes/acre in year 4

New Research Project Objectives 1) To test innovative super-high density citrus grove replanting configurations with a drip fertigation open hydroponics (OH) system for maximizing early return on investment in a canker and HLB-endemic disease environment. 2) To develop sustainable high yielding OH solutions for highbush blueberry cultivation in Florida which will eliminate the need for expensive mulching with pine bark.

1) Citrus APS Planted 08/2012 Main source of N: 1) calcium nitrate 2) ammonium nitrate Rootstocks: 1) Swingle 2) US897 538 trees/acre All drip fertigated (2x0.5 gph/tree) 484 trees/acre

Tramline planting 538 trees/acre = 9 x ½(3+15) feet

Measurements: Soil ph in the drip zone, and leaf and soil nutrients obtained from quarterly sampling SPAD leaf color, tree height and canopy width measured quarterly Electrical conductivity in the fertigated root zone Fruit yield and quality measured annually from year 2 (manual harvesting of a subsample of each plot). External (color) and internal (brix, juice %, acid %) quality will be measured

November 2012, Lake Alfred, Fl

Rootstock Rootstock Fertilizer x Fert Fertilizer Fertilizer Rootstock Rootstock Rootstock Rootstock Fertilizer Rootstock Rootstock Rootstock *** ** *** *** *** ** *** ** *** *** *** *** *

Rootstock x Fert Rootstock x Fert Rootstock x Fert Rootstock x Fert Rootstock x Fert Rootstock x Fert Rootstock, Fert Rootstock x Fert Rootstock, Fert Rootstock x Fert Rootstock, Fert ** *** *** * *** *** ** *** *** *** *** *** ***

Leaf tissue analysis: Feb 2014 (N,P,K,Ca,Mg,S,Mn,Zn,Fe,B,Cu) Rootstk*** US897 Swingle Fert*** CaN AN N% 3.073 3.197 3.213 3.074 Rootstk*** US897 Swingle K% 1.027 1.487 Rootstk* US897 Swingle Fert*** CaN AN Ca% 4.272 3.604 4.016 3.764 Rootstk*** US897 Swingle B(ppm) 121.6 154.4 Rootstk*** US897 Swingle Zn(ppm) 36.89 31.90 Rootstk*** US897 Swingle Mn(ppm) 62.43 46.42 Rootstk*** US897 Swingle Cu(ppm) 14.73 18.34

Valencia /Sw tramlines after 1 year

13 months

2.5 years

2.5 years

2.5 years

2.5 years 30% HLB symptomatic Stunted tree canopies Loss of leaves Fruit defects Fruit drop

Conclusions after 2.5 years Drip fertigation accelerated tree growth in years 1-2 CaN fertilizer grew marginally larger canopies CaN fertilizer ensured better foliar Ca and N nutrition >30% HLB+ by end 2014, regardless of treatment At a doubling of infection every year, 100% infection expected by year 4-5 and economic breakeven not achievable Other technologies like covered production are being tested in new fertigation trials to totally prevent HLB infection

2) Blueberry APS Treatments: Blueberry bushes grown in native mineral soil with standard NPK fertigation (ammonium nitrogen) Blueberry bushes grown in standard mulched pine bark beds and standard NPK fertigation (ammonium nitrogen) Blueberry bushes grown in native mineral soil with incorporated pine bark, and standard NPK fertigation (ammonium nitrogen) Blueberry bushes grown in native mineral soil with enhanced NPK fertigation (nitrate and ammonium) and comprehensive micronutrient supply, PLUS dissolved organic leached derivatives of pine bark delivered through fertigation Southern Highbush blueberry, Farthing variety

Photo courtesy of Wije Baranayake Roots prefer to grow in the pine bark only

Using pine bark for highbush blueberry cultivation in Florida: Pine bark mulch is an essential component for production of highbush blueberries grown in FL Low ph of pine bark High organic matter content Photo courtesy of Wije Baranayake Problems: Needs replacement ~3-4 yrs Restricted rooting zone Irrigating pine bark is difficult High cost Can customized liquid fertigation substitute for pine bark? Soluble pine bark extract?

-- 4 -- -- 4 -- -- 4 -- -- 4 -- -------------------- 31+1+1 ----------------------- ---33 --- N Main Plot 24 experimental units ----------------------------------------------------;174 ------------------------------------------------------ 12 23 34 4 21 21 22 23 24 Structure of one plot (Experimental Unit) 3 3 ------------------------------ 6 ------------------------------- X ------2 ------- X X X X X X X Sampling plants within a plot 3 X X X X 3 X X X X 3 X X X X 5 rows of blueberry in each plot with between row spacing = 4 (sampling from middle 3 rows)

12 pine bark treatment

5 months after planting 10 months after planting Tank containing pine bark-filled mesh bags prior to filling up with water Soluble leachate from the bark was fertigated as part of treatment 4

Results: measurements from 2014 season Soil ph measurements after 5 months Soil ph Treatment Description Treatment Plot Initial 1 Native soil 5.77 (0.05) 5.76 (0.07) 2 Pine bark 5.70 (0.27) 5.65 (0.03) 3 Pinebark mixed with soil 5.84 (0.18) 5.66 (0.06) 4 Native soil + Pinebark extract 5.78 (0.13) 5.61 (0.07) Blueberry needs a soil ph below 5 for optimum performance. Therefore, elemental sulfur at a rate of 250 kg ha -1 was broadcast during mid March in an attempt to adequately acidify the soil The weed fabric covering the blueberry plots prevented proper efficacy of the sulfur Therefore an acidifying ammonium-based blueberry fertilizer ( 21:7:7 acidifier ) was used thereafter in all the treatments

N P K Mg Ca S g kg -1 T1 15.9 b 1.20 b 9.18 a 2.25 a 8.00 a 1.42 a T2 21.8 a 1.52 a 6.38 c 1.42 c 4.18 c 1.42 a T3 21.4 a 1.47 a 6.02 c 1.43 c 4.48 c 1.45 a T4 19.8 ab 1.32 b 8.18 b 1.82 b 6.27 b 1.50 a Sufficient Range Leaf nutrient concentrations: major & secondary 17-21 0.8-4.0 4-6.5 1.5-3.0 3.0-8.0 1.2-2.0

Leaf nutrient concentrations: micro B Zn Mn Fe Cu mg kg -1 T1 42.7 a 74.5 a 106 a 118 a 120 a T2 24.2 c 40.3 b 79 a 63 b 81 b T3 26.0 c 39.3 b 94 a 65 b 92 b T4 34.5 b 78.8 a 98 a 110 a 127 a 25-70 8-30 50-350 60-200 5-30

Treatment Leaf gas exchange measurements Water Use Efficiency Chlorophyll Index (SPAD) Photosynthesis Conductance Transpiration Aug. 29 Oct. 1 Aug. 29 Oct. 1 Aug. 29 Oct. 1 Aug. 29 Oct. 1 Oct. 1 mmol m -2 s -1 mol m -2 s -1 mmol m -2 s -1 ratio index 1 9.9 b 13.2 0.14 b 0.21 4.5 6 2.2 2.21 53.2 b 2 12.5 a 14.1 0.17 a 0.36 5.4 7.4 2.3 2.17 57.3 a 3 12.3 a 14.6 0.17 a 0.25 5.4 7.1 2.3 2.15 57.3 a 4 9.9 b 12.4 0.15 ab 0.41 5 8.3 2 1.73 56.5 a NS NS NS NS NS NS

Plant canopy measurements Plant Height (cm) Treatment 5/13/2014 6/13/2014 7/21/2014 9/5/2014 10/1/2014 T1 58.8 a 57.39 a 58.3 c 58.4 b 60.6 c T2 62.3 a 64.44 a 71.2 a 80.0 a 82.0 b T3 60.5 a 65.72 a 72.8 a 84.7 a 89.7 a T4 62.9 a 62.03 ab 62.0 bc 61.0 b 61.5 c Number of Branches Treatment 5/13/2014 6/13/2014 7/21/2014 9/5/2014 10/1/2014 T1 2.9 a 3.69 d 7.4 d 17.4 b 26.5 b T2 3.4 a 6.89 ab 15.8 b 38.7 a 51.1 a T3 3.2 a 8.06 a 18.3 a 38.3 a 55.4 a T4 3.2 a 4.11 cd 7.6 d 19.1 b 24.9 b

Conclusions after 1.5 years & future work Blueberries grew best in pine bark treatments Weak growth in mineral soil related to low leaf N? The pine bark extract had a non-significant impact Follow-up work will investigate rhizosphere differences in the treatments; mineralizable N Aerobic extracts of pine bark will be tested versus previous anaerobic extracts

Acknowledgements