Good Use Practices for Kasumin, and other new tools for managing fire blight

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Good Use Practices for Kasumin, and other new tools for managing fire blight Kerik D. Cox NYSAES Plant Pathology and Plant-Microbe Biology Section School of Integrative Plant Science Cornell University

Outline Kasugmycin (Kasumin 2L) Other chemical tools Biologicals Coppers Fire blight product efficacy Fire blight chemical management plan

Kasugamycin (Kasumin 2L) Aminoglycoside antibiotic developed as rice blast fungicide (protectant) Same class but different MoA: inhibits protein production Resistance: mutations in 16S rrna methyltransferase ksga gene No resistance reported in E. amylovora Resistance found in other environmental bacteria APSnet.org Wikipedia commons

Kasugamycin (Kasumin 2L) Evaluated in 1980s for fire blight: testing suspended for phytotoxicity Arysta Lifescience s Kasumin 2L: New formulation safe for apples 2010-2014 seasons: section 18 label for MI 2015 season: section 3 label for US, NYSDEC is expediting NY request

Outline Kasugmycin (Kasumin 2L) Other chemical tools Biologicals Coppers Fire blight product efficacy Fire blight chemical management plan

Serenade Optimum A.I. & M. O. A.: Bacillus subtilis-antibiotic metabolites Diseases: Fire blight & apple scab, anthracnose, botrytis, rusts My experiences Fungal diseases: sooty blotch, fly speck, & rusts: moderate Fire blight: >50% control at heavy pressure & 100% control light pressure Wikipedia commons

DoubleNickel55/LC A.I. & M.O.A/: Bacillus amyloliquefaciens strain D747-antibiotic metabolites Diseases: fire blight & foliar & fruit diseases My experiences Fungal diseases: sooty blotch, fly speck, & rusts: moderate to high Fire blight: >50% control at heavy pressure & 100% control light pressure Wikipedia commons

Blossom protect A.I. & M.O.A.: Aureobasidium pullulans strains x2 = competitive inhibition of stigmatic surface Diseases: fire blight Known experiences and concerns 50-80% control of fire blight under high pressure Fruit russeting shouldn t happen 80% bloom

Copper products MasterCop: Copper sulfate pentahydrate 5.4% MCE Bloom rate + 1-3 lbs./hyrdated lime Experiences: Effective on fire blight (50-75% control Mixing issues with strep Phyto./russeting 1 year

Copper products Cueva: Copper Octanoate (Copper Soap) 1.8%MCE, OMRI Bloom rate, but guidelines issues with label text Experiences: effective on sooty blotch flyspeck late season, no phyto issues

Badge X2 (OMRI): Copper Oxychloride & Hydroxide 28% MCE Bloom rate + 1-3 lbs./hydrated lime Experiences: Copper products Effective on fire blight (75% control) and fly speck sooty blotch late season No mixing or phyto. Issues Enhanced strep

Outline Kasugmycin (Kasumin 2L) Other chemical tools Biologicals Coppers Fire blight product efficacy Fire blight chemical management plan

Managing FB: product efficacy Orchard site 4-5 year old Idared trees on B.9 Artificial inoculum (Ea 273 at 1x10 7-8 CFUml -1 ) Spray for BB or Scissor dip for SB

Managing FB: product efficacy Blossom blight application timing Pre-bloom timings for biopesticides All antibiotics & biopesticides @ 80% bloom Blossom blight incidence: percentage of blighted blossoms (5 reps)

Managing FB: product efficacy Shoot blight application timing Active terminal growth (5-7 ): 24 hours after inoculation (trauma) Apogee (PF/1-2 ) or 5- days prior: Actigard Shoot blight: progression of canker of 20 shoots (5 reps)

2012 Blossom Blight Trial High pressure year: Streptomycin programs, Strep + low copper, Kasumin 2L, Blossom Protect

2013 Blossom Blight Trial Low pressure year: Streptomycin programs, Strep + low copper, Kasumin, Blossom Protect, Bloomtime Biological

2014 Blossom Blight Trial Moderate pressure year: Antibiotic programs, Blossom Protect, MasterCop, BmJ & Double Nickel

2012 Shoot Blight 2012: Only Apogee and the two antibiotics provided a significant reduction of shoot blight progression

2013 Shoot Blight 2013: Only Apogee and the two antibiotics provided a significant reduction of shoot blight progression

2014 Shoot Blight 2014: Antibiotics provided strongest reduction of shoot blight progression; biologicals, Apogee, and Actigard > good effect

Blossom Blight Summary Streptomycin greatest activity against BB Improved by bloom rate of buffered copper: No phyto! Kasugamycin 2L (protectant):effective as strep Resistant management: not necessary in region where SmR Ea not confirmed or suspected Biologicals & Low MCE coppers They work, but more effective against lower inoculum levels & variable in performance Often equivalent to oxytet (does not kill)

Shoot Blight Summary Antibiotics greatest effect on trauma shoot blight Don t use antibiotics for shoot blight outside trauma events Apogee Even single application provides considerable control: important for high vigor varieties Copper & Actigard Variable in performance, and strongest effect against realistic inoculum levels

Fire blight management at Bloom Watch for CCE alerts and disease model forecasts for fire blight infection periods (NEWA & MaryBlyt 7.1) If SmR Ea has been confirmed at your operation: 1. When the first blossom infection is forecast, apply Kasumin 2L 2. At the 2 nd high risk period, apply a tank mix of streptomycin with either oxytetracycline or a bloom time rate of a registered copper product 3. At the 3 rd or 4 th high risk periods, repeat steps 1 and 2, respectively

Fire blight management at Bloom Watch for alerts and disease model forecasts for fire blight infection periods (NEWA & MaryBlyt 7.1) If SmR Ea has not been confirmed at your operation: 1. When the first blossom infection is forecast, apply a tank mix of streptomycin with either oxytetracycline or a bloom time rate of a registered copper product 2. At the 2 nd high risk period, apply Kasumin 2L 3. At the 3 rd or 4 th high risk periods, repeat steps 1 and 2 depending on concerns about the effectiveness of streptomycin

Acknowledgments State, federal, and institutional funds appropriated to the New York State Agricultural Experiment Station Funding support by the NYS Apple Research and Development Program New York State Department of Agriculture & Markets - Specialty Crop Block Grant Summer Crew!

Questions? Erwinia amylovora PCR confirmation 0 ppm copper 125 ppm copper