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THE Clifton RANGE Unstirred Baths NE1B Series Boiling Baths NE1 Series Thermostatic Baths NE1-9 Shallow Form Bath IMPORTANT: ALWAYS FILL the water bath before connecting to the power supply.

Contents Location 4 Unpacking 4 Safety 4 Assembly 4 Power Supply Lead and Connection to Electrical Supply 4 Liquid Level 4 Suitable Liquids 4 Operating Instructions 5 Cleaning 8 Descaling 8 Virucidal Disinfectant 8 Warranty - 3 Years 9 Portable Appliance Testing 9 Accessories 10 Fitting Accessories 11 Service and Parts List 12 Specification 13 Declaration of Conformity 16 Final Inspection and Electrical Safety Test Report Enclosed 2 The Clifton Range - 3 Year Warranty High Quality products at affordable prices

About this Manual This user Manual contains instructions which must be followed in order that the product is operated correctly. General Notes Please observe the following safety precautions: 1. Fill the tank prior to connection to power supply. 2. Connect only to a power supply with the corresponding voltage to that specified on the rating label positioned on the rear of the unit. 3. Ensure the power supply has a safety earth (ground) terminal. 4. Ensure the mains switch and power supply connector are accessible during use. 5. The mains supply cord fitted to this products is a heat resistant type and should be replaced by an equivalent type. 6. Do not block ventilation slots during use and follow installation instructions. 7. Always follow good laboratory practice by ensuring substances being heated present no risk of a hazard (explosion, implosion or release of toxic or flammable gases) or that these have been addressed. When heating substances where liberation of gases occurs suitable extraction should be used. 8. Use only liquids specified in this Instruction Manual within their specified temperature range. 9. Use caution when topping up or draining the tank as the liquid in the tank may be very hot or cold. Allow the liquid to cool to 40 C before draining. 10. Drain before moving the bath. 11. Recommend using a Lid above 60 C. Take care when lifting the lid - steam and hot vapours can cause scalding. 12. Use a thermometer to check the temperature - do not touch liquid. 13. If this product is not used in accordance with these instructions, then basic safety protection afforded by the water bath may be affected. 14. Before using any cleaning or decontamination method except those recommended, check with your distributor that the proposed method will not damage the equipment. Amendments Issue 1 July 1991 Initial issue instruction book. Issue 2 February 1993 Revised added NE1-28 version. Issue 3 June 1993 NE1B range introduced. Issue 4 August 1994 Instruction book revised layout. Issue 5 May 1995 CE version. Issue 6 September 1995 Multi-ligual version. Issue 7 May 1996 Update address details. Issue 8 November 1997 NE1-22/28, NE1B-14 fuse revision. Issue 9 September 1999 F to T. Issue 10 July 2000 Complete update. Issue 11 July 2001 Product updates for 2002. Issue 12 September 2004 Revised instruction book. Issue 13 July 2005 New NE1-9, NE1B range updated constant level and MCB s. Issue 14 July 2006 NE1B-14 1000W to 1200W and Note front cover filling bath. Symbols Caution refer to Instructions Protective earth conductor terminal. The Clifton Range - 3 Year Warranty High Quality products at affordable prices 3

Location The surface on which the product is to be located should be smooth, level and sturdy. Use in a ventilated room. Ambient room temperature 5 C to 40 C. Maximum humidity 80% for temperature 31 C decreasing to 50% at 40 C. Product is designed for laboratory use. Unpacking Remove the product from its packaging. Any damage to the product notify your dealer immediately. Retain packaging over warranty period. Contents consist of a bath, stainless steel false base, power lead and instruction manual. Safety Do not touch any electrical contacts or open any closure panels RISK OF ELECTRICAL SHOCK! Assembly Place the false base inside the bath. Fit the power lead into the socket at rear. Power Supply Before attempting to connect the product to an electrical supply check the information on the rating label is compatible. IF IN DOUBT CONSULT AN ELECTRICIAN. THE PRODUCT MUST BE EARTHED!. Where the mains supply or plug differs refer to local regulations or qualified electrician. Liquid Level - NE1 range Minimum liquid level - must cover the top of the false base by 40mm Maximum liquid level - must not exceed the ridge in the tank. Suitable Liquids - NE1 range Always ensure the product is disconnected from the electrical supply when emptying and filling. Operating temperatures from ambient +5 C to 99 C, for general use we recommend either: Distilled water. Heat transfer liquid [The LB range is formulated for temperatures from -45 C to 90 C and provides complete protection from freezing and algae growth and safeguards against corrosion. See accessories for the full range available]. VIRKON dissolved in distilled water has proven efficacy against bacteria (including mycobacteria), viruses, spores and fungi in a variety of independent tests using different protocols. Liquid Level - NE1B range Minimum and maximum water level is governed by the constant level which maintains water level during boiling. Using standard laboratory tubing, connect to the bottom outlet of the constant level to a regulated water supply/tap, adjust the water supply to provide a slow feed of water and maintaining boiling and this automatically maintains safe water level. The side outlet acts as an overflow to a sink or drain. Suitable Liquids - NE1B range Normal tap water is suitable since a maintained water supply is required. 4 The Clifton Range - 3 Year Warranty High Quality products at affordable prices

Operating Instructions NE1B series Front view Clifton Low height shelf needs less water for same work saving energy and costs. Also allows tall samples to be immersed to either a greater working depth. Dial, adjusts level of boiling. Indicator: heating. Splash proof controls. Mains power switch I ON, O OFF. Drain outlet for accessory tap: 28 litre model. Rear views Serial number and electrical supply details. Power supply lead and plug. MCB fuses Normal use requires no action. Fault situation, buttons will pop out disconnecting electrical supply. SEEK Guidance before resetting - push to reset. Constant level device, maintains liquid level during boiling. Outlet, connect with laboratory tubing to a drain or waste outlet. Inlet, connect with laboratory tubing to fill the Boiling bath then adjust the flow water supply, to maintain liquid level The Clifton Range - 3 Year Warranty High Quality products at affordable prices 5

Operating Instructions - NE1 series Front view Clifton Low height shelf needs less water for same work saving energy and costs. Also allows tall samples to be immersed to either a greater working depth. Dial, thermostatic control, set to desired temperature. Indicator: heating. Splash proof controls. Mains power switch I ON, O OFF. Drain outlet for accessory tap: 22, 28 and 56 litre models. Rear view Serial number and electrical supply details. IEC connector, for power supply lead and plug. Fuse draw fitted underneath. 6 The Clifton Range - 3 Year Warranty High Quality products at affordable prices

NE1B Series: Operating Instructions 1. Fill the bath with water following instructions on Page 4 Liquid Level - NE1B range and diagram on Page 5. 2. Connect the mains plug to an electrical supply. 3. Turn unit on using mains switch located on the front of the unit. 4. Heater indicator will illuminate to show heater activity. 5. Adjust the blue knob to control the degree of boil from gentle simmer to vigorous boil. NE1 Series: Operating Instructions 6. Allow the water temperature to stabilise in the bath, and take an actual temperature reading. 1. Fill the bath with water observing recommended levels on Page 4 Liquid Level - NE1 range 2. Connect the mains plug to an electrical supply. 3. Turn unit on using mains switch located on the front of the unit. 4. Adjust the temperature control to the desired setting using the blue knob. 5. Heater indicator will illuminate to show heater activity. 6. Allow the water temperature to stabilise in the bath, and take an actual temperature reading. Adjust the temperature controller accordingly. The Clifton Range - 3 Year Warranty High Quality products at affordable prices 7

Cleaning General cleaning Important - please follow these instructions to avoid possible damage to the unit, otherwise affect its performance and or warranty. Disconnect the product from the electrical supply before cleaning. The water bath must be cleaned at regular intervals wiping casework with a cloth or sponge soaked in warm soapy water. The stainless steel crevice free tank with smooth corners should provide years of valuable service and is resistant to chloride containing solutions it is however important to avoid high concentrations of halogens - especially chloride. With such a high quality and resistant tank it may show symptoms of these halogens as rust, which are deposits from external sources in the water supply. We recommend always empty the bath of liquid after use and wipe out the internal faces of the tank with a non-abrasive cloth and allow to dry. Any deposits can be removed with nitric acid (10%) on a cloth. WEAR PROTECTIVE EQUIPMENT! It is also recommended to use an accessory lid to prevent contaminates landing in bath liquids. Descaling Descale the stainless steel tank regularly to maintain it in as new condition ensuring the corrosion resistance and normal operating conditions are maintained throughout its working life. Descale by adding 1 litre of vinegar to water and gently heating to 50 C for an hour, empty, and brush the lime away. Rinse thoroughly afterwards. Virucidal Disinfectant We recommend Virkon a powder product for the safe and rapid disinfection of surfaces and equipment in a wide variety of situations available from your distributor.. VIRKON is dissolved in water for use, providing a safe working solution with a faint lemon odor. It has proven efficacy against bacteria (including mycobacteria), viruses, spores and fungi in a variety of independent tests using different protocols. Presents no serious long term health risks to staff obviating the need for costly ventilation equipment and health monitoring. Also provides high level disinfection of laboratory equipment and instruments where autoclaving is neither practical nor necessary. The ultimate high level surface disinfectant. Its chemistry is a balanced stabilized blend of peroxygen compounds, surfactant, organic acids and an inorganic buffer system. Mode of action is by oxidation of proteins and other components of cell protoplasm, resulting in inhibition of enzyme systems and loss of cell wall integrity. Virkon is Registered in accordance with the requirements of the Medical Devices Directive, (93/42/ EEC) as a Medical Device. 8 The Clifton Range - 3 Year Warranty High Quality products at affordable prices

3 Year Warranty Our service engineers are fully trained in the assembly, calibration and servicing of all Clifton instrumentation. Products can be returned to our comprehensively equipped service centre where a fast and efficient turnaround is guaranteed: Service Department, Nickel Electro Limited, Oldmixon Crescent, Weston-super-Mare, North Somerset BS24 9BL, UK. Tel +44 (0)1934 626691 Fax +44 (0)1934 630300. Out of Warranty Our Service Department has comprehensive stock of charegeable spare parts maintaining working life of equipment or units can be returned for quotation before repairs are undertaken. End of Life This symbol indicates that this product should not be disposed of with your waste. Instead, dispose waste electrical equipment by handing it over to a designated collection point for the recycling of waste electrical and electronic equipment. The separate collection and recycling of your waste equipment at the time of disposal will help to conserve natural resources and ensure that it is recycled in a manner that protects human health and the environment. For more information about where you can drop off your waste equipment for recycling, in UK please contact Service Department, rest Europe contact your Distributor. Health & Safety, unless in receipt of a Decontamination Notice or Report the unit cannot be returned or accepted for disposal. Clifton electrical and electronic equipment has been designed for recycling and takes into account the dismantling and recovery its components and materials. Clifton products are easily recycled with majority of the product constructed from stainless or mild steels, which can readily be re-used or recycled. In excess of 85% of this product range can be easily re-cycled economically. Portable Appliance Testing When conducting testing, ensure it is conducted by a qualified person. DO NOT PAT TEST THE BATH UNLESS IT CONTAINS WATER. THIS EQUIPMENT MUST NOT BE FLASH TESTED! The Clifton Range - 3 Year Warranty High Quality products at affordable prices 9

ACCESSORIES FOR NE2D SERIES GRP GABLE LIDS GL1-4 GRP Gable Lid to suit 4 Litre Unstirred Baths GL1-14 GRP Gable Lid to suit 14 Litre Unstirred Baths GL1-22 GRP Gable Lid to suit 9, 22 and 28 Litre Unstirred Baths STAINLESS STEEL GABLE LIDS SL1-4 Stainless Steel Gable Lid to suit 4 Litre Unstirred Baths SL1-8 Stainless Steel Gable Lid to suit 8 Litre Unstirred Baths SL1-14 Stainless Steel Gable Lid to suit 14 Litre Unstirred Baths SL1-22 Stainless Steel Gable Lid to suit 9, 22 and 28 Litre Unstirred Baths SL1-22H Stainless Steel Hinged Gable Lid to suit 22 + 28 Litre Unstirred Baths STAINLESS STEEL FLAT LIDS LD-4 Flat One Piece Stainless Steel Lid to suit 4 Litre Capacity Bath LD-8 Flat One Piece Stainless Steel Lid to suit 8 Litre Capacity Bath LD-14 Flat One Piece Stainless Steel Lid to suit 14 Litre Capacity Bath LD-22 Flat One Piece Stainless Steel Lid to suit 9, 22 and 28 Litre Capacity Bath SA01368 Flat One Piece Stainless Steel Lid to suit 56 litre bath (two required) STAINLESS STEEL FLAT LIDS WITH CONCENTRIC RINGS SLR1-4 4 x 105mm Stainless Steel Ringed Lid to suit 4 Litre Unstirred Baths SLR1-8 4 x 83mm Stainless Steel Ringed Lid to suit 8 Litre Unstirred Baths SLR1-14 4 x 105mm Stainless Steel Ringed Lid to suit 14 Litre Unstirred Baths SLR2-14 6 x 83mm Stainless Steel Ringed Lid to suit 14 Litre Unstirred Baths SLR1-22 6 x 105mm Stainless Steel Ringed Lid to suit 9, 22 and 28 Litre Unstirred Baths STAINLESS STEEL TEST TUBE RACKS - DIMENSIONS 270 X 70 X 138MM (L X W X H) 6870 Stainless Steel Test Tube Rack 26 Hole x 17mm Diameter 6871 Stainless Steel Test Tube Rack 16 Holes x 26mm Diameter 6872 Stainless Steel Test Tube Rack 36 Holes x 13mm Diameter 6873 Stainless Steel Test Tube Rack 18 Holes x 19mm Diameter/suitable for 1.5ml microtubes 6900 Stainless Steel Test Tube Rack 12 Holes x 32mm Diameter Note: 4 Litre = 1 Rack, 8 Litre = 2 Racks, 14 Litre = 4 Racks, 22 and 28 Litre = 6 Racks MISCELLANEOUS LB-2.5 2.5 Litres - Lab Bath 4590 - Heat transfer fluid - Temperature range -15 to +90 C LB-5.0 5 Litres - Lab Bath 4590 - Heat transfer fluid - Temperature range -15 to +90 C TC-1 Thermometer Clip Complete With Bent Stem Spirit Filled Thermometer BX0616 Draining Syphon BX0688 Drain Tap BP0368 Polypropylene Spheres For more information on Accessories www.nickel-electro.co.uk 10 The Clifton Range - 3 Year Warranty High Quality products at affordable prices

FITTING ACCESSORIES FOR NE2D SERIES GRP GABLE LIDS Position the lid over the bath and lower into place. The lids are designed to fit snugly so that condensate runs into the bath. STAINLESS STEEL GABLE LIDS Position the lid over the bath and lower into place. These lids have bo drip points, provide full working height across the bath when fitted and have a ventilation gap. For H suffix lids place the lid onto the bath with the hinges hanging over the rear of the water bath aliigning with threaded bushes. Insert two screws provided into each hinge and tighten screws. The lid can be raised and lowered using the handle on the side. STAINLESS STEEL FLAT LIDS Position the lid over the bath and lower into place. STAINLESS STEEL FLAT LIDS WITH CONCENTRIC RINGS Position the lid over the bath and lower into place. Each set of rings can be used to vary the diameter of aperture to accommodate different sizes of vessels of evaporating dishes and glassware, including round-bottom flasks. STAINLESS STEEL TEST TUBE RACKS Place the loaded test tube rack into the bath on top of either the stainless steel perforated shelf or the raised shelf. Note: 4 Litre = 1 Rack, 8 Litre = 2 Racks, 14 Litre = 4 Racks, 22 and 28 Litre = 6 Racks HEAT TRANSFER FLUID Use as a neat liquid solution for best results. THERMOMETER CLIP This can be positioned anywhere on the perimeter of the bath by cliping over the edge. The thermometer then lies flush with the top edge of the bath to prevent accidental damage. DRAINING SYPHON Allows the easy and quick emptying of any water bath. Water temperature must be below 45 C before emptying can commence. DRAIN TAP A drain tap outlet is featured on the 22, 28 and 56 litre sizes. Make sure the bath is empty, hold the outer nut, then using a socket/allen key undo the threaded insert. Once removed and still holding the outer nut, screw in the accessory drain tap into position and pinch tight. POLPROPYLENE SPHERES Place on top of the water/oil to provide a floating lid reducing evaporation and insulating the bath reducing heat losses. The range of Unstirred water baths: 4 litre bath requires 1 pack; 8 litre bath requires 2 pack; 14 litre 2 packs and 9, 22 and 28 litre requires 4 packs. The Clifton Range - 3 Year Warranty High Quality products at affordable prices 11

Service Diagrams and Spare Parts - NE1 Service Diagrams and Spare Parts - NE1B 12 The Clifton Range - 3 Year Warranty High Quality products at affordable prices

NE1B range Clifton Boiling Baths, water, unstirred Designed for applications that require continuous boiling. This range also has the flexibility to allow the temperature to be regulated from a gentle simmer to a vigorous boil. Comprising of a corrosion resistant stainless steel working environment, with heater and safety cut out concealed underneath providing a totally clear clutter free working area, all housed in a durable, chemical resistant, powder coated paint finished outer case. Integral constant level device for connection to water supply to combat evaporation of water and maintain boiling. Featuring Clifton Low height removable stainless steel shelf increasing working volume. Drain outlet on 28 litre model for convenient bath emptying Heating element with safety cut-out concealed underneath the bath Cat No. NE1B-8 NE1B-14 NE1B-28 Capacity, litres 8 14 28 Heater power, W 800 1200 1500 Drain Outlet No No Yes Safety Fixed cut out Fixed cut out Fixed cut out Internal Dims, mm (wxdxh) 240x300x150 325x300x150 500x300x200 Overall Dims, mm (wxdxh) 332x270x290 361x332x290 538x332x290 Voltage 230V or 120V The Clifton Range - 3 Year Warranty High Quality products at affordable prices 13

NE1 range Clifton Baths, water, thermostatic, unstirred A broad range of unstirred analogue baths designed for general use and economically priced without comprising quality. Comprising of a corrosion resistant stainless steel working environment using a 304 spec (18/8) stainless steel tank with heater and safety cut out concealed underneath providing a totally clear clutter free working area, all housed in a durable, chemical resistant, powder coated paint finished outer case. Easy to use thermostatic control, graduated in 5 C increments, with illuminated on/off switch and heater on indicator. Featuring removable unique Clifton Low height removable stainless steel shelf, water saving feature and reducing energy consumption or increasing working volume. Side lifting handles for ease of transportation. Drain outlet on larger volumes 22, 28 and 56 litre models for safe and convenient bath emptying. Temperature factory calibrated @ 45 C for consistency. Complementary range of accessories including racks and lids. Cat No. NE1-2.5 NE1-4 NE1-8 NE1-14 NE1-22 NE1-28 NE1-56 Capacity, L 2.5 4 8 14 22 28 56 Temperature Ambient +5 C - 100 C Sensitivity Uniformity ±0.25 C ±0.1 C Heater, W 250 400 800 1000 1500 1500 2500 Drain Outlet No No No No Yes Yes Yes Internal, mm 230x130x100 300x150x150 300x240x150 325x300x150 500x300x150 500x300x200 620x500x200 Overall, mm 270x190x240 332x185x290 332x270x290 361x332x290 538x332x290 538x332x290 660x540x290 Voltage 230V or 120V 14The The Clifton Clifton Range Range - 3 Year - 3 Year Warranty Warranty High High Quality Quality products products at affordable at affordable prices prices7

NE1 range Clifton Baths, shallow form, water, thermostatic, unstirred A shallow height unstirred water bath designed for operating with low liquid levels suitable for small bottles, tubes and micro plates. Perfect for heating samples in Microcentrifuge tubes and molecular biology Low profile design allowing easy access and visibility of work in the bath Unlike traditional high sided baths, small containers and samples are not obscured Cat No. NE1-9 Capacity, litres 9 Temperature range Ambient +5 C - 100 C Sensitivity Uniformity ±0.25 C ±0.1 C Heater power, W 1000 Drain Outlet Internal Dims, mm (wxdxh) Overall Dims, mm (wxdxh) Voltage No 500x300x65 535x332x135 230V or 120V The Clifton Range - 3 Year Warranty High Quality products at affordable prices 15

EC Declaration of Conformity We herewith confirm the following product: NE1B and NE1 Series of Unstirred WaterBaths Conform with the requirements outlined by the following European Directives: Low Voltage Directive (73/23/EEC) EMC Directive (89/336/EEC) Conforms with the requirements of following standards: BS EN 61010:1:1993 BS EN 61010:2.010:1995 Safety requirements for electrical equipment for measurement, control and laboratory use. BS EN 61326:1997 Electrical equipment for measurement control and laboratory use - EMC requirements. Nickel-Electro Ltd, Oldmixon Crescent, Weston-s-Mare, North Somerset, BS24 9BL, England t 01934 626691 f 01934 630300 16 The Clifton Range - 3 Year Warranty High Quality products at affordable prices

Final Inspection and Electrical Safety Test Report 2BX0842 www.nickel-electro.co.uk