ACQUITY Photodiode Array Detector with TaperSlit Overview and Maintenance Guide

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General Information Copyright notice 2013 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER. The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com). Trademarks ACQUITY, Waters, and THE SCIENCE OF WHAT S POSSIBLE. are registered trademarks of Waters Corporation, and Advanced Polymer Chromatography, APC, and TaperSlit are trademarks of Waters Corporation. PEEK is a trademark of Victrex Corporation. Teflon is a registered trademark of E.I. dupont de Nemours and Company. Other registered trademarks or trademarks are the sole property of their owners. May 28, 2013, 715004023 Rev. A iii

Customer comments Waters Technical Communications organization invites you to report any errors that you encounter in this document or to suggest ideas for otherwise improving it. Help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability. We seriously consider every customer comment we receive. You can reach us at tech_comm@waters.com. Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information: Contacting medium Internet Telephone and fax Conventional mail Information The Waters Web site includes contact information for Waters locations worldwide. Visit www.waters.com. From the USA or Canada, phone 800 252-4752, or fax 508 872 1990. For other locations worldwide, phone and fax numbers appear in the Waters Web site. Waters Corporation 34 Maple Street Milford, MA 01757 USA iv May 28, 2013, 715004023 Rev. A

Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, or radiological hazards (or any combination thereof). You must know the potentially hazardous effects of all substances you work with. Always follow Good Laboratory Practice, and consult your organization s safety representative for guidance. Considerations specific to the photodiode array (PDA) detector High voltage hazard Warning: To avoid electric shock, do not remove the detector's protective panels, which cover components serviceable only by Waters technicians. FCC radiation emissions notice Changes or modifications not expressly approved by the party responsible for compliance, could void the users authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. Canada spectrum management emissions notice This class A digital product apparatus complies with Canadian ICES-001. Cet appareil numérique de la classe A est conforme à la norme NMB-001. Electrical power safety notice Do not position the instrument so that it is difficult to operate the disconnecting device. May 28, 2013, 715004023 Rev. A v

Safety hazard symbol notice Documentation needs to be consulted in all cases where the symbol is used to find out the nature of the potential hazard and any actions which have to be taken. Equipment misuse notice If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Safety advisories Consult Appendix A for a comprehensive list of warning and caution advisories. Operating this device When operating this device, follow standard quality-control (QC) procedures and the guidelines presented in this section. Applicable symbols Symbol Definition Manufacturer Authorized representative of the European Community Confirms that a manufactured product complies with all applicable European Community directives Australia C-Tick EMC compliant vi May 28, 2013, 715004023 Rev. A

Symbol Definition Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements Consult instructions for use Electrical and electronic equipment with this symbol may contain hazardous substances and should not be disposed of as general waste. For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. Audience and purpose This guide is intended for personnel who install, operate, and maintain the ACQUITY PDA detector with TaperSlit (PDA-TS). It gives an overview of the detector s technology and operation. Intended use of the photodiode array (PDA) detector Waters designed the ACQUITY PDA-TS detector to analyze and monitor many compounds used in the ACQUITY Advanced Polymer Chromatography (APC ) system. The ACQUITY PDA-TS detector is not intended for use in diagnostic applications. May 28, 2013, 715004023 Rev. A vii

ISM classification ISM Classification: ISM Group 1 Class B This classification has been assigned in accordance with IEC CISPR 11 Industrial Scientific and Medical (ISM) instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment. Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network. EC authorized representative Waters Corporation (Micromass UK Ltd.) Floats Road Wythenshawe Manchester M23 9LZ United Kingdom Telephone: +44-161-946-2400 Fax: +44-161-946-2480 Contact: Quality manager viii May 28, 2013, 715004023 Rev. A

Table of Contents Copyright notice... iii Trademarks... iii Customer comments... iv Contacting Waters... iv Safety considerations... v Considerations specific to the photodiode array (PDA) detector... v FCC radiation emissions notice... v Canada spectrum management emissions notice... v Electrical power safety notice... v Safety hazard symbol notice... vi Equipment misuse notice... vi Safety advisories... vi Operating this device... vi Applicable symbols... vi Audience and purpose... vii Intended use of the photodiode array (PDA) detector... vii ISM classification... viii ISM Classification: ISM Group 1 Class B... viii May 28, 2013, 715004023 Rev. A ix

EC authorized representative... viii 1 ACQUITY Photodiode Array Detector with TaperSlit... 13 Detector optics... 14 Calculating absorbance... 16 Flow-cell operating principles... 17 Resolving spectral data... 18 Measuring light at the photodiode array... 19 Optimizing the signal-to-noise ratio... 21 Selecting the appropriate sampling rate... 21 Filtering data... 22 Computing absorbance data points... 25 Dark current... 25 Reference spectrum... 25 Data averaging... 26 Reference wavelength compensation... 28 2 Setting up the Detector... 29 Before you begin... 30 Installing the detector... 31 Plumbing the detector... 33 Installing the multi-detector drip tray... 37 Making Ethernet connections... 39 I/O signal connectors... 39 Connecting to the electricity source... 40 3 Preparing the Detector... 41 Starting the detector... 42 Monitoring detector function... 44 x May 28, 2013, 715004023 Rev. A

Detector control panel... 45 Shutting down the detector... 46 Shut down for fewer than 24 hours... 46 Shut down for more than 24 hours... 46 4 Maintaining the Detector... 49 Contacting Waters Technical Service... 50 Maintenance considerations... 50 Safety and handling... 50 Proper operating procedures... 51 Flushing the detector... 52 Maintaining the leak sensor... 53 Correcting leak-sensor errors... 53 Replacing the leak sensor... 57 Maintaining the flow cell... 59 Flushing the flow cell... 59 Replacing the flow cell... 60 Replacing the lamp... 62 Replacing fuses... 65 Cleaning the instrument s exterior... 66 A Safety Advisories... 67 Warning symbols... 68 Specific warnings... 69 Caution advisory... 71 Warnings that apply to all Waters instruments and devices... 72 Warnings that address the replacing of fuses... 77 Electrical and handling symbols... 79 Electrical symbols... 79 Handling symbols... 80 May 28, 2013, 715004023 Rev. A xi

B Specifications... 81 Physical specifications... 82 Environmental specifications... 82 Electrical specifications... 83 Performance specifications... 84 Wetted materials of construction... 86 C Materials of Construction and Compatible Solvents... 87 Prevent contamination... 88 Items exposed to solvent... 88 Solvents used to prepare mobile phases... 88 Mobile phase absorbance... 89 D Spectral Contrast Theory... 93 Comparing absorbance spectra... 94 Representing spectra as vectors... 95 Vectors derived from two wavelengths... 96 Vectors derived from multiple wavelengths... 97 Spectral contrast angles... 97 Undesirable effects... 100 Detector noise... 101 Photometric error... 101 Solvent changes... 101 Threshold angle... 101 xii May 28, 2013, 715004023 Rev. A

1 ACQUITY Photodiode Array Detector with TaperSlit The Waters ACQUITY photodiode array detector with TaperSlit (PDA-TS) is an ultraviolet/visible (UV/Vis) spectrophotometer designed for use in the ACQUITY Advanced Polymer Chromatography (APC ) system. The detector, controlled by Empower software, operates as an integral part of the ACQUITY APC system. To use the detector s operating software effectively, you must understand the principles that underlie operation of the detector s optics and electronics. Contents: Topic Page Detector optics 14 Calculating absorbance 16 Flow-cell operating principles 17 Resolving spectral data 18 Measuring light at the photodiode array 19 Optimizing the signal-to-noise ratio 21 Selecting the appropriate sampling rate 21 Filtering data 22 Computing absorbance data points 25 May 28, 2013, 715004023 Rev. A 13

1 ACQUITY Photodiode Array Detector with TaperSlit Detector optics The detector operates within a range of between 190 and 800 nm. Its array of 512 photodiodes produces an optical resolution of 1.2 nm. The figure illustrates the light path through the optics assembly. Optics assembly light path: Grating Window Order filter Photodiode array M1 mirror Slit Spectrograph mirror and mask Lamp Filter flag/shutter Flow cell TP02819 14 May 28, 2013, 715004023 Rev. A

Detector optics The following table describes the optics assembly components. Optics assembly components: Component Lamp M1 mirror Window Filter flag/shutter Flow cell Spectrograph mirror and mask Slit Grating Order filter Photodiode array Function Deuterium source lamp. Focuses light from the deuterium source lamp. Used to help minimize air infiltration into the lamp housing. Flags positions for measuring open (sample) and blocked (dark) beam energies and wavelength verification. Houses the segment of the flow path (containing eluent and sample) through which the polychromatic light beam passes. The mirror focuses light transmitted through the flow cell onto the slit at the entrance to the spectrographic portion of the optics. The mirror mask defines the size of the beam at the grating. Determines wavelength resolution and intensity of light striking the photodiodes. The width of the slit is 50 µm. Disperses light into bands of wavelengths and focuses them onto the plane of the photodiode array. Reduces the contribution of second-order diffraction of UV light (less than 370 nm) to the light intensity observed at visible wavelengths (greater than 370). A linear array of 512 photodiodes. The diode width (50 µm), together with a 50-µm slit, yields single wavelength resolution of 1.2 nm. May 28, 2013, 715004023 Rev. A 15

1 ACQUITY Photodiode Array Detector with TaperSlit Calculating absorbance An algorithm computes absorbance by subtracting the dark current (see Dark current on page 25) from the reference spectrum (reference energy) and the acquired spectrum (sample energy). Absorbance is based on the principles of Beer s law. Beer s law The Beer-Lambert law (commonly called Beer s law) describes the relationship between the quantity of light of a particular wavelength arriving at the photodiode and the concentration of the sample passing through the flow cell. Beer s law is expressed as A = εlc where A = dimensionless quantity measured in absorbance units ε = constant of proportionality known as the molar extinction coefficient l = path length, in centimeters (1.0 cm in the detector s normal flow cell) c = concentration in moles per liter Beer s law applies only to well-equilibrated dilute solutions. It assumes that the refractive index of the sample remains constant, that the light is monochromatic, and that no stray light reaches the detector element. As concentration increases, the chemical and instrumental requirements of Beer s law are sometimes violated, resulting in a deviation from (absorbance versus concentration) linearity. The absorbance of mobile phase can reduce the linear range. 16 May 28, 2013, 715004023 Rev. A

Flow-cell operating principles Absorbance as a function of concentration: Ideal Actual Absorbance Linear range for analyte Concentration Flow-cell operating principles The Waters TaperSlit flow cell used in the ACQUITY PDA-TS detector renders the detector baseline essentially insensitive to changes in mobile phase refractive index (RI). RI changes occur during gradient separations or result from temperature or pump-induced pressure fluctuations. To achieve RI immunity, a combination of a spherical mirror, a lens at the entrance of the flow cell, and a taper to the internal bore of the flow cell prevents light rays from striking the internal walls of the flow cell. The Waters TaperSlit flow cell, so-called because of the shape of the flow cell exit face, matches the shape of the spectrograph slit. Compared to a conventional flow cell with a cylindrical shape, the detector achieves higher light throughput for a given spectral resolution with the TaperSlit cell design. May 28, 2013, 715004023 Rev. A 17

1 ACQUITY Photodiode Array Detector with TaperSlit Comparison of flow cell characteristics: Conventional flow cell: UV light Window Window TaperSlit analytical flow cell: Window UV light Lens Resolving spectral data Together with photodiode spacing, the detector s 50-µm slit determines the intensity and bandwidth of the light that strikes the photodiode array. Reducing the bandwidth increases the resolving power of the detector. As a result, similar spectra are more effectively distinguished. The grating images the slit onto the photodiode array. The angle of diffraction from the grating determines the wavelength that strikes a particular photodiode in the array. The following figure shows an absorbance spectrum of benzene. Note that the wavelength resolution is sufficient to resolve five principal absorption peaks. 18 May 28, 2013, 715004023 Rev. A

Measuring light at the photodiode array Benzene spectrum at different resolutions: 3.6 nm Absorbance 1.2 nm 230.00 250.00 270.00 nm Measuring light at the photodiode array The detector measures the amount of light striking the photodiode array, to determine the absorbance of the sample in the flow cell. The array consists of a row of 512 photodiodes. Each photodiode acts as a capacitor initially holding a fixed amount of charge. Light striking a photodiode discharges the diode. The magnitude of the discharge depends on the amount of light striking the photodiode. May 28, 2013, 715004023 Rev. A 19

1 ACQUITY Photodiode Array Detector with TaperSlit Photodiodes discharged by light: Sample in flow cell absorbs at specific wavelengths. Deuterium lamp Light from grating dispersed onto diodes. Grating Flow cell Slit Mirror The detector measures the amount of charge required to recharge each photodiode. The charge is proportional to the amount of light transmitted through the flow cell over the interval specified by the diode exposure time. 20 May 28, 2013, 715004023 Rev. A

Optimizing the signal-to-noise ratio Optimizing the signal-to-noise ratio Optimizing signal-to-noise ratios involves choosing an acquisition wavelength range that includes only the wavelengths of interest. Significantly, the range must be one in which the mobile phase absorbs only minimally. You can improve the signal-to-noise ratio by increasing the spectral resolution value. For example, you can choose to operate at 3.6 nm instead of at 1.2 nm resolution. Note that the filter-time constant and sampling rate affect the signal-to-noise ratio. Selecting the appropriate sampling rate A sufficient number of points must fall across a peak to define its shape. Thus, the definition between peaks is lost at very low sampling rates. Empower software applies end time minus the start time to calculate the points-acrosspeak value for each integrated peak in the chromatogram. Tip: The points-across-peak value appears in the Peaks table, at the bottom of the Review Main window. If the Points Across Peak field is not visible, right-click anywhere in the table, and then click Table Properties. Click the Columns tab, and then scroll down to find the Points Across Peak field. Clear the check box, and then click OK. If the points-across-peak value for the narrowest peak of interest is less than 25, specify a higher sampling rate in the instrument method. If the value is greater than 50, specify a lower sampling rate. May 28, 2013, 715004023 Rev. A 21

1 ACQUITY Photodiode Array Detector with TaperSlit Example of how baseline noise increases with higher sampling rates: 5 Hz 10 Hz 20 Hz 40 Hz Filtering data On the General tab of the PDA Instrument Method Editor, you can apply an optional noise filter (via the Digital Filtering parameter) to the data acquired. See also: The Empower online Help. The detector uses a Hamming filter to minimize noise. The filter is a digital, finite-impulse-response filter that creates peak-height degradation and enhances the filtering of high frequency noise. The behavior of the filter depends on the filter-time constant that you select. Increasing the constant reduces baseline noise, improving the signal-to-noise ratio. Nevertheless, increasing the constant too much artificially broadens the peak and reduces chromatographic resolution. You can choose among these options when programming a filtering time: fast, slow, normal, or other. If you select a fast, slow, or normal filtering time, you need not specify a value, because the sampling rate determines the filtering constant. If you select the Other option, you can specify a value. Nevertheless, the value you enter is rounded, up or down, to a value based on the sampling rate. Selecting Other and entering a value of 0.0 disables all filtering. 22 May 28, 2013, 715004023 Rev. A

Filtering data The following table lists the digital filter settings for the allowable data rates. Digital filter settings for data rates: Sampling rate Slow Normal Fast 1 4.000 2.000 1.000 2 2.000 1.000 0.500 5 0.800 0.400 0.200 10 0.400 0.200 0.100 20 0.200 0.100 0.050 40 0.100 0.050 0.025 80 0.050 0.025 0.0125 Lower filter-time constant settings produce these effects: Narrow peaks, with minimal peak distortion and time delay Very small peaks, which can be difficult to discriminate from baseline noise Suppression of less baseline noise Higher filter-time constant settings produce these effects: Greatly decreased baseline noise Shortened and broadened peaks For the highest resolution, select an appropriate sampling rate for your separation, and choose a fast filter-time constant. For the highest sensitivity, select an appropriate sampling rate for your separation, and choose a normal filter-time constant. The following figure shows the relationship between increased filter-time constant and absorbance. May 28, 2013, 715004023 Rev. A 23

1 ACQUITY Photodiode Array Detector with TaperSlit Filter-time constant comparison: Absorbance 0 sec 1 sec 2 sec Time (minutes) Although the peak shape shows some distortion and the signal output is delayed with different filter-time constants, the peak area remains unchanged. 24 May 28, 2013, 715004023 Rev. A

Computing absorbance data points Computing absorbance data points An algorithm calculates the absorbance data points and transmits them to the database (Empower software). The absorbance calculation is where S = sample energy D = dark energy R = reference energy t = elapsed time after start of injection λ = wavelength This value is then filtered as specified. Dark current Photodiodes discharge even when they are not exposed to light. This discharge is called dark current. The dark current is updated by closing the shutter to take a dark current reading for each diode. After updating, the detector opens the shutter and then subtracts the dark current values as shown in the above equation. Reference spectrum R λ D λ Absorbance t, λ = Log 10 ----------------------- S t, λ D λ The reference spectrum is a measure of lamp intensity and mobile phase absorbance at initial conditions. The detector records a reference spectrum at the start of each injection. The reference spectrum value is calculated using the same filter-time constant as the absorbance data. May 28, 2013, 715004023 Rev. A 25

1 ACQUITY Photodiode Array Detector with TaperSlit Data averaging The data the detector report to the database (Empower software) can be the average of several data points. After calculating absorbance, the detector averages absorbance values according to the requested spectral resolution. Average spectral resolution The detector can simultaneously collect two types of data channels: spectra (3D) and chromatograms (2D). For best results in library matching and peak purity analysis, set the 3D resolution to 1.2 nm. For chromatograms (2D data), select a resolution to optimize signal amplitude, baseline noise, and linear dynamic range. When the monitoring wavelength for an analyte corresponds to a peak s lambda max, increasing bandwidth tends to decrease peak height while reducing baseline noise and linear dynamic range. Tip: A resolution of 3.6 nm is effective for many analytes. 26 May 28, 2013, 715004023 Rev. A

Computing absorbance data points Resolution comparison for anthracene: 0.55 0.50 0.45 0.40 0.35 Absorbance 0.30 0.25 0.20 0.15 0.10 0.05 0.00 3.20 3.30 3.40 3.50 3.60 3.70 3.80 3.90 4.00 4.10 4.20 252 nm at 1.2 nm, height 0.53 252 nm at 6.0 nm, height 0.45 252 nm at 12.0 nm, height 0.35 Average chromatographic sampling rate Sample rate is the number of data points acquired per second. The number of times a given pixel is read during the sample rate interval depends on its exposure time. For example, if exposure time is 25 msec and sample rate is 20 Hz (20 samples/second), then exposures per sample is calculated according to this equation: 20 samples/second = = 2 exposures/sample (1000 msec/second) (20 samples/second)(25 msec/exposure) May 28, 2013, 715004023 Rev. A 27

1 ACQUITY Photodiode Array Detector with TaperSlit The readings are averaged and reported as a single data point. The baseline noise decreases as the number of exposures/sample increases. Tip: The amount of data stored is based on wavelength range, spectral resolution, runtime, and sample rate. Specify these parameter values in the General tab of the PDA Instrument Method Editor. For details, refer to the Empower online Help. Reference wavelength compensation A compensated reference wavelength collects wide-band absorbance data in a region of the spectra where no known analytes exist. It is used to reduce detector drift and wander, which can affect integration quality. The detector calculates the compensation value by averaging the absorbance values within a range of wavelengths that you select. It then subtracts that value from the absorbance value: Abs-Comp(t) = Abs(t) - CRef(t) where Abs-Comp = absorbance-compensated Abs = absorbance CRef = compensation reference t = elapsed time after start of injection A starting and ending wavelength defines the compensation reference. CRef bandwidth must be 40 nm and 100 nm and fall within a range of 190 to 800 nm. Recommendation: Select a compensation reference range where no analytes are expected to appear. Since responses are subtracted from the absorbance value, any responses within the compensation reference range can erroneously affect quantitative data. 28 May 28, 2013, 715004023 Rev. A

2 Setting up the Detector Contents: Topic Page Before you begin 30 Installing the detector 31 Plumbing the detector 33 Installing the multi-detector drip tray 37 Making Ethernet connections 39 Connecting to the electricity source 40 May 28, 2013, 715004023 Rev. A 29

2 Setting up the Detector Before you begin To install the detector, you must generally know how to set up and operate laboratory instruments and computer-controlled devices and how to handle solvents. Tip: Consult this guide in conjunction with the ACQUITY APC system documentation and online Help. Before installing the detector, ensure that these requirements and conditions are met: The detector is not situated under a heating or cooling vent. The required components are present. The shipping containers or unpacked items are not damaged. If you discover any damage or discrepancy when you inspect the contents of the cartons, immediately contact the shipping agent and your local Waters representative. Customers in the USA and Canada must report damage and discrepancies to Waters Technical Service (800 252-4752). Others must phone their local Waters subsidiary or Waters corporate headquarters in Milford, Massachusetts (USA), or they may visit http://www.waters.com. For complete information on reporting shipping damages and submitting claims, see Waters Licenses, Warranties, and Support Services. 30 May 28, 2013, 715004023 Rev. A

Installing the detector Installing the detector Warning: To avoid spinal or muscular injury, if only one person installs the detector, he or she must do so using a mechanical lift. To install the detector: 1. Place the rear feet of the detector atop the previously added module in the system stack, and slide it backward until its rear alignment pin rests in the rear alignment slot on the module. Alignment pin (2) Alignment slot (2) 2. Lower the front of the detector so that its front alignment pin rests in the front alignment slot on the previously added module. 3. Place the solvent tray module atop the detector. May 28, 2013, 715004023 Rev. A 31

2 Setting up the Detector Location of the ACQUITY PDA-TS detector in a system: Solvent bottle tray Detector Column manager Sample manager Solvent manager 32 May 28, 2013, 715004023 Rev. A

Plumbing the detector Plumbing the detector Caution: To avoid severely damaging the detector by using incompatible solvents, consult Appendix C of the ACQUITY APC System Guide. Plumbing the detector involves connecting the flow cell and installing a backpressure regulator, if necessary. Although the in-line degasser removes most of the gas (air) from solvents, some gas is reintroduced during partial-loop injections. Under pressure, this gas remains in solution. However, because the post-column pressure is normally much lower than the pre-column pressure, the gas may come out of solution and produce an unstable baseline characterized by large, unexpected spikes. A backpressure regulator maintains a minimum post-column pressure of 1724 kpa (17 bar, 250 psi), eliminating post-column outgassing and ensuring a smooth baseline. If the ACQUITY PDA-TS detector is the last detector in the system, the backpressure regulator is required for optimum performance. Recommendation: To avoid particulate contamination in the flow cell, flush any columns you intend to connect to the detector before connecting them. See also: ACQUITY APC System Guide. To plumb the detector: 1. If the detector is already powered on, in the console, select PDA Detector from the system tree, and click (Lamp Off), to extinguish the lamp. 2. Square the flow cell assembly in front of the opening, and then insert it into the optics bench. Note that the flow cell uses the alignment pins on the optics bench. 3. Gently push the front of the assembly until it seats on the alignment pin. May 28, 2013, 715004023 Rev. A 33

2 Setting up the Detector Alignment pin Flow cell handle 4. Continue to insert the flow cell until the three thumbscrews align with their holes in the bulkhead. Caution: To prevent the flow cell from binding and to ensure that it is properly seated in the bulkhead, alternate between tightening the captive screws and pushing the flow cell forward. 5. Hand tighten the thumbscrews, and then tighten them snug with the 1/4-inch flat-blade screwdriver. 34 May 28, 2013, 715004023 Rev. A

Plumbing the detector 6. Connect the stainless steel tubing from the column manager to the flow cell inlet. Lamp Flow cell Handle Flow cell outlet Flow cell inlet Flow cell assembly May 28, 2013, 715004023 Rev. A 35

2 Setting up the Detector 7. Attach the short length of outlet tubing from the backpressure regulator to the flow cell outlet. Important: Follow the direction of flow when installing the backpressure regulator. Direction of flow from outlet To waste TP03260 8. Route the long end of the outlet tubing from the backpressure regulator, through the channel clips along the front, right-hand side of the system, and into a suitable waste container. Warning: To avoid the harmful effects of personal contact (including inhalation) with biohazards or compounds that are toxic or corrosive, ensure the end of the outlet tube is secured properly to the waste container, to contain solvent vapors. Important: Ensure the solvent used in the current system is compatible with the backpressure regulator tubing. 36 May 28, 2013, 715004023 Rev. A

Installing the multi-detector drip tray Installing the multi-detector drip tray If your ACQUITY APC system includes more than one detector, you must install the multi-detector drip tray. ACQUITY PDA-TS detector installed in a split ACQUITY APC system: Column manager Sample manager ACQUITY PDA-TS detector Solvent manager ACQUITY RI detector Required materials Multi-detector drip tray kit T10 Torx driver T20 Torx driver 5/16-inch open-end wrench To install the drip tray: 1. Turn the ACQUITY PDA-TS detector so that it rests on its left-hand side. 2. Snap the extended plastic feet on to the bottom of the detector, and then snap the anti-skid pads onto the extended plastic feet. May 28, 2013, 715004023 Rev. A 37

2 Setting up the Detector 3. Secure the drip tray to the bottom of the detector using the plastic rivets provided in the multi-detector drip tray kit. Drain fitting Plastic rivets Alignment studs 4. Install the barbed drain fitting into the end of the drip tray. 5. Replace the existing (short) alignment studs with longer studs on the base of the ACQUITY PDA-TS detector: Using a 5/16-inch open-end wrench, remove the two existing short alignment studs from the bottom of the detector. Replace the studs with the longer studs from the kit. 6. Return the ACQUITY PDA-TS detector to its original position atop the other detector, following the installation procedure described on page 31. 38 May 28, 2013, 715004023 Rev. A

Making Ethernet connections Making Ethernet connections To make connections: 1. Unpack and install the preconfigured workstation. 2. Connect one end of one Ethernet cable to the network switch, and then connect the other end to the Ethernet card, on the workstation. Tip: On preconfigured systems, the Ethernet card is identified as the Instrument LAN card. 3. Connect one end of one Ethernet cable to the back of the detector, and then connect the other end to the network switch. I/O signal connectors The rear panel of the detector includes a removable connector that holds the screw terminals for the optional I/O signal cables. This connector is keyed so that it can be inserted only one way. Refer to the signal connection location shown on the silk-screened label affixed to the rear panel of the detector. I/O signal connectors: 1 2 3 4 5 6 Inject Start + Inject Start - Switch Switch Analog Out + Analog Out - ACQUITY PDA-TS detector analog-out/event-in/event-out connections: Signal connection Inject Start Switch Analog Out Description Indicates (with a contact closure output) that an injection has started. Output switch to trigger external devices. Analog data output. May 28, 2013, 715004023 Rev. A 39

2 Setting up the Detector Connecting to the electricity source The ACQUITY PDA-TS detector requires a separate, grounded electricity source. The ground connection in the electrical outlet must be common and connected near the system. Recommendation: Use a line conditioner and uninterruptible power supply (UPS) for optimum long-term input voltage stability. Warning: To avoid electrical shock, observe these precautions: Use power cord SVT-type in the United States and HAR-type (or better) in Europe. For other countries, contact your local Waters distributor. Power-off and unplug the detector before performing any maintenance on the instrument. Connect all components of the ACQUITY APC system to a common ground. To connect to the electricity source: 1. Connect the female end of the power cord to the receptacle on the rear panel of the detector. 2. Connect the male end of the power cord to a suitable wall outlet. 40 May 28, 2013, 715004023 Rev. A

3 Preparing the Detector Contents: Topic Page Starting the detector... 42 Shutting down the detector... 46 May 28, 2013, 715004023 Rev. A 41

3 Preparing the Detector Starting the detector Caution: To avoid severely damaging the detector by using incompatible solvents, consult Appendix C of the ACQUITY APC System Guide. Starting the detector entails individually powering-on each system module including the detector and the workstation. It also entails starting the operating software (Empower software). Caution: To minimize contaminants that can leave deposits on the flow cell s walls, flush new columns for 15 minutes before connecting the flow cell. If you must power-on the detector before the eluent is flowing, extinguish the lamp. You can do this in the Instrument Method Editor (Empower software) by specifying a Lamp On event in the Events table. You can also extinguish the lamp in one of these ways: In Empower software, click (Lamp Off) in the control panel at the bottom of the Run Samples window. In the console, select PDA Detector from the system tree, and click (Lamp Off). See also: ACQUITY APC System Guide. To start the detector: 1. Power-on the workstation. 2. Press the power switch on the top left-hand side of the isocratic solvent manager door and sample manager door. Result: Each system instrument beeps and runs a series of startup tests. 42 May 28, 2013, 715004023 Rev. A

Starting the detector The power and lamp LEDs change as follows: Each system instrument s power LED shows green. During initialization, each system instrument s status LED flashes green. After the instruments are successfully powered-on, all LEDs show steady green. The isocratic solvent manager s flow LED and the sample manager s run LED remain unlit. 3. Start Empower software. Tip: You can monitor the ACQUITY console for messages and LED indications. 4. Flush the system with filtered, degassed, and sparged HPLC-grade methanol or acetonitrile. 5. In the console, set the isocratic solvent manager to deliver the appropriate flow for the flow cell in your system. Tip: Use only thoroughly degassed HPLC-grade solvents. Gas in the mobile phase can form bubbles in the flow cell and cause the detector to fail the reference energy diagnostic test. 6. Pump mobile phase for at least 15 minutes. 7. Ensure the detector cell is filled with solvent and free of bubbles. Important: The detector can fail to initialize correctly if the cell contains air. 8. Press the power switch on the front panel, to power-on the detector. Result: The detector runs a series of startup diagnostic tests while the lamp LED blinks green. The lamp LED shows steady green when the lamp is ignited. 9. When the lamp LED is steady green, start Empower software, and download an instrument or inlet method. Result: The ACQUITY Console displays messages and visual signals. 10. For best results, wait one hour for the detector to stabilize before acquiring data. May 28, 2013, 715004023 Rev. A 43

3 Preparing the Detector Monitoring detector function Light emitting diodes on the detector indicate its state of functioning. Power LED The power LED, on the left-hand side of the detector s front panel, indicates when the detector is powered-on or powered-off. Lamp LED The lamp LED, to the right of the power LED, indicates the lamp status. Lamp LED indications: LED mode and color Unlit Constant green Flashing Green Flashing red Constant red Description Indicates the detector lamp is extinguished. Indicates the detector lamp is ignited. Indicates the detector is initializing or calibrating. Indicates an error stopped the detector. Information regarding the error that caused the failure can be found in the console. Indicates a detector failure that prevents further operation. Power-off the detector, and then power-on. If the LED is still steady red, contact your Waters service representative. 44 May 28, 2013, 715004023 Rev. A

Starting the detector Detector control panel If Empower software controls the system, the detector s control panel appears at the bottom of the Run Samples window. Detector control panel: Status Lamp On/Off LED Shutter position Turn detector lamp On/Off The detector control panel displays the acquisition status and shutter position. You cannot edit detector parameters while the system is processing samples. The following table lists the items in the detector control panel. Modifiable detector control panel items: Control panel item Lamp On/Off LED Status Shutter (Lamp On) (Lamp Off) Description This image mimics the actual lamp on/off LED mode unless communications with the detector are lost. Clicking it opens the lamp control window. Displays the status of the current operation. Displays the shutter position (Open, Closed, Erbium, or UV blocking). Ignites the detector lamp. Extinguishes the detector lamp. May 28, 2013, 715004023 Rev. A 45

3 Preparing the Detector You can access additional functions by right-clicking anywhere in the detector control panel. Additional functions in the detector control panel: Control panel function Autozero Reset PDA Help Description Resets the detector offsets. Resets the detector, when present, after an error condition. Displays the console Help. Shutting down the detector Shutdown options vary according to how long the detector will remain unused. Shut down for fewer than 24 hours If you expect a few hours to pass before the next injection, slow the flow rate in the interim to a few tenths of a milliliter per minute, to conserve solvent. Keep the detector operating and the column heater at operating temperature during this period. To shut down the detector: 1. Continue to pump the initial mobile phase mixture through the column. Tip: Doing so maintains the column equilibrium necessary for good retention time reproducibility. 2. To lengthen lamp life, extinguish the detector lamp by clicking (Lamp Off) in the detector control panel. Shut down for more than 24 hours To shut down the detector: 1. Extinguish the detector lamp by clicking (Lamp Off) in the detector control panel. 2. Remove buffer salts and additives by flushing with water. 3. Flush the column and flow cell with 100% pure organic solvent. 46 May 28, 2013, 715004023 Rev. A

Shutting down the detector See also: ACQUITY APC Columns Care and Use Manual. Warning: To avoid electric shock, completely interrupt electrical power to a system module by setting its On/Off switch to the Off position. Then remove the module's power cable from the wall outlet. Although the On/Off switch on each module controls the basic operational state of the module, some circuits remain live after the module is switched off. 4. Power-off the system. Alternative: If you prefer to leave the system powered-on, turn off the column heater, or reduce its temperature setting to 40 C. Important: Before using any system or instruments that have been shut down under the recommended conditions, ensure that the new mobile phase and solvents are miscible with the recommended storage solvents: water/methanol or water/acetonitrile. If they are not directly miscible with the recommended storage solvents, use an intermediate solvent that is miscible with both the storage solvents and the new-analysis solvents to flush the storage solvents from the system. 5. Cap the flow cell inlet and outlet ports. May 28, 2013, 715004023 Rev. A 47

3 Preparing the Detector 48 May 28, 2013, 715004023 Rev. A

4 Maintaining the Detector Contents: Topic Page Contacting Waters Technical Service... 50 Maintenance considerations... 50 Maintaining the leak sensor... 53 Maintaining the flow cell... 59 Replacing the lamp... 62 Replacing fuses... 65 Cleaning the instrument s exterior... 66 May 28, 2013, 715004023 Rev. A 49

4 Maintaining the Detector Contacting Waters Technical Service If you are located in the USA or Canada, report malfunctions or other problems to Waters Technical Service (800 252-4752). Otherwise, phone the Waters corporate headquarters in Milford, Massachusetts (USA), or contact your local Waters subsidiary. Our Web site includes phone numbers and e-mail addresses for Waters locations worldwide. Go to www.waters.com. When you contact Waters, be prepared to provide this information: Any error messages Nature of the symptom Instrument serial numbers Flow rate Operating pressure Solvent(s) Detector settings (sensitivity and wavelength) Type and serial number of column(s) Sample type Empower software version and serial number Workstation model and operating system version For complete information on reporting shipping damages and submitting claims, see Waters Licenses, Warranties, and Support Services. Maintenance considerations Safety and handling Observe these warning and caution advisories when you perform maintenance on your detector. Warning: To prevent injury, always observe good laboratory practices when you handle solvents, change tubing, or operate the system. Know the physical and chemical properties of the solvents you use. See the Material Safety Data Sheets for the solvents in use. 50 May 28, 2013, 715004023 Rev. A

Maintenance considerations Warning: To avoid electric shock, do not remove the detector's top panel, which covers components serviceable only by Waters technicians. Caution: To avoid damaging the electronic components or circuitry of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source. Follow this procedure to completely interrupt power to the module: 1. Set the module s power switch to Off. 2. Disconnect the module s electrical supply cord from the ac source. Afterward, wait 10 seconds before disconnecting any electrical assembly from the module. To prevent circuit damage due to static charges, do not touch integrated circuit chips or other system instruments that do not require manual adjustment. Proper operating procedures To ensure your system runs efficiently, follow the operating procedures and guidelines presented in Chapter 3. Spare parts Replace only the component parts mentioned in this document. For details about spare parts, use the Waters Quality Parts Locator on the Waters Web site s Services & Support page. Recommendations: To prevent dirt from getting into the optics assembly, always keep the detector door closed whenever a flow cell is not installed in the detector. Filter and degas solvents, to prolong column life, reduce pressure fluctuations, and decrease baseline noise. To conserve lamp life, extinguish the lamp while leaving the detector running but idle. Note, however, that you should do so only when the lamp would remain extinguished more than 4 hours. If you use buffered mobile phase, flush it from the detector before powering-off the detector. Doing so prevents these adverse events: Plugging solvent lines and the flow cell May 28, 2013, 715004023 Rev. A 51

4 Maintaining the Detector Damaging instrument components Microbial growth Important: To ensure optimum performance of the flow cell, the eluent must be flowing before you power-on the detector. Nevertheless, if you must power-on the detector before the eluent is flowing, extinguish the lamp before doing so. If the flow cell will not be used for more than 24 hours, flush it with clean mobile phase, such as a water/acetonitrile or water/methanol mix. Then cap the flow ports or dry the flow cell with pure nitrogen or pure helium for 5 to 10 minutes. Caution: To avoid damaging the detector or column, remove the column and disconnect the detector before you flush the system. Flushing the detector Caution: To avoid damaging the flow cell, do not exceed the 6895 kpa (69 bar, 1000 psi) pressure limitation of the flow cell. To flush the detector: 1. Remove the column from the system. 2. Flush the system to waste with 100% HPLC-quality water at 1.0 ml/min for 10 minutes. Note: In some cases, intermediate solvents must be used. 3. Flush the system with a solution of 90:10 methanol/water for 10 minutes. 52 May 28, 2013, 715004023 Rev. A

Maintaining the leak sensor Maintaining the leak sensor A leak sensor in the drip tray continuously monitors the detector for leaks. The sensor stops system flow when it detects accumulated, leaked liquid in its surrounding reservoir, and an error message describing the problem appears in the Instrument Console. Correcting leak-sensor errors After approximately 1.5 ml of liquid accumulates in the leak sensor reservoir, an alarm sounds, indicating that the leak sensor detected a leak. Warning: To avoid personal contamination with biohazards, compounds that are toxic or corrosive, or the residues of such compounds, wear clean, chemical-resistant, powder-free gloves when handling the leak sensor. Caution: To avoid scratching or damaging the leak sensor, do not allow buffered solvents to accumulate and dry on it; do not submerge it in a cleaning bath. Required materials Clean, chemical-resistant, powder-free gloves Cotton swabs Nonabrasive, lint-free wipes To correct a leak-sensor error: 1. View the Leak Sensors dialog box in the Instrument Console to verify that the leak sensor detected a leak. Tip: If a leak is detected, a Leak Detected error message appears. 2. Open the detector door, gently pulling its right-hand edge toward you. 3. Locate the source of the leak, and make the repairs necessary to stop the leak. May 28, 2013, 715004023 Rev. A 53

4 Maintaining the Detector Caution: To avoid damaging the leak sensor, do not grasp it by the ribbon cable. 4. Remove the leak sensor from its reservoir by grasping it by its serrations and pulling upward on it. Serrations Tip: If you cannot easily manipulate the leak sensor after removing it from its reservoir, detach the connector from the front of the instrument (see page 57). 5. Use a nonabrasive, lint-free wipe to dry the leak sensor prism. Prism 54 May 28, 2013, 715004023 Rev. A

Maintaining the leak sensor 6. Roll up a nonabrasive, lint-free wipe, and use it to absorb the liquid from the leak sensor reservoir and its surrounding area. Rolled up lint-free wipe Leak sensor reservoir 7. With a cotton swab, absorb any remaining liquid from the corners of the leak sensor reservoir and its surrounding area. Cotton swab Leak sensor reservoir May 28, 2013, 715004023 Rev. A 55

4 Maintaining the Detector 8. Align the leak sensor s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. T-bar TP03559 TP02908 Slot in leak sensor reservoir Leak sensor installed in reservoir 9. If you detached the connector from the front of the instrument, reattach it. 10. In the Instrument Console, select your detector from the system tree. 11. In the detector information window, click Control > Reset to reset the detector. 56 May 28, 2013, 715004023 Rev. A

Maintaining the leak sensor Replacing the leak sensor Required materials Clean, chemical-resistant, powder-free gloves Leak sensor To replace the leak sensor: Warning: To avoid personal contamination with biohazards, compounds that are toxic or corrosive, or the residues of such compounds, wear clean, chemical-resistant, powder-free gloves when handling the leak sensor. 1. Open the detector door, gently pulling its right-hand edge toward you. 2. Press down on the tab to detach the leak sensor connector from the front of the instrument. Press down on tab to release connector Leak sensor connector 3. Remove the leak sensor from its reservoir by grasping it by its serrations and pulling upward on it. Serrations 4. Unpack the new leak sensor. May 28, 2013, 715004023 Rev. A 57

4 Maintaining the Detector 5. Align the leak sensor s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. TP03559 T-bar TP02908 Slot in leak sensor reservoir Leak sensor installed in reservoir 6. Plug the leak sensor connector into the front of the instrument. 7. In the Instrument Console, select your detector from the system tree. 8. In the detector information window, click Control > Reset to reset the detector. 58 May 28, 2013, 715004023 Rev. A

Maintaining the flow cell Maintaining the flow cell The flow cell requires maintenance when these events occur: the reference spectrum changes. the cell fluid leaks out of the drain tube. the detector cannot initialize but the lamp is in good condition. the detector causes high backpressure. Tip: Conditions other than a dirty flow cell can also cause decreased lamp intensity. Flow cell maintenance consists of these tasks: flushing the flow cell. removing the flow cell. disassembling and cleaning the flow cell. installing the flow cell assembly. Flushing the flow cell Required Materials HPLC-grade water HPLC-grade methanol If the flow cell requires cleaning, first try flushing it with solvent. To flush the flow cell: 1. Select a solvent compatible with the samples and mobile phases that you have been using. If you have been using buffers, flush with 10 ml of HPLC-grade water, then flush with 10 ml of a low-surface-tension solvent such as methanol. Tip: Ensure that the solvent is miscible with the previous mobile phase. 2. Test the lamp energy by performing the Read energy diagnostic test. If the lamp diagnostic test fails and the lamp has not been used more than 2000 hours or 1 year from date of purchase (whichever comes first), call Waters Technical Service (see page 50). May 28, 2013, 715004023 Rev. A 59

4 Maintaining the Detector Replacing the flow cell Required materials 1/4-inch flat-blade screwdriver Flow cell To replace the flow cell: 1. Power-off the detector. 2. Stop the solvent flow. 3. Open the door. 4. Disconnect the column outlet tubing from the flow cell inlet. 5. Disconnect the waste line from the flow cell outlet. Lamp Flow cell handle Flow cell Thumbscrews 6. Remove the flow cell: Use a 1/4-inch flat-blade screwdriver to loosen the three thumbscrews on the flow cell assembly s front plate. Grasp the handle and gently pull it toward you. 60 May 28, 2013, 715004023 Rev. A

Maintaining the flow cell 7. Unpack and inspect the new flow cell. 8. Square the flow cell assembly in front of the opening, and then insert it into the optics bench. Note that the flow cell uses the alignment pins on the optics bench. 9. Gently push the front of the assembly until it seats on the front alignment pins. Alignment pin Flow cell handle 10. Continue to insert the flow cell until the three thumbscrews align with their holes in the bulkhead. Caution: To prevent the flow cell from binding and to ensure that it is properly seated in the bulkhead, alternate between tightening the captive screws and pushing the flow cell forward. 11. Hand tighten the thumbscrews, and then tighten them snug with the 1/4-inch flat-blade screwdriver. 12. Connect the column outlet tubing to the flow cell inlet. 13. Connect the waste line to the flow cell outlet. May 28, 2013, 715004023 Rev. A 61

4 Maintaining the Detector 14. Close the door. 15. Before you power-on the detector, prime the system to fill the flow cell with solvent and remove any air. Replacing the lamp Change the lamp when it repeatedly fails to ignite or when the detector fails to calibrate. The detector source lamp is warrantied to light and pass startup diagnostic tests for 2000 hours or 1 year from the date of purchase, whichever comes first. Note, however, that if you do not record a new lamp s serial number in the Instrument Console, the date of the previous lamp installation remains in the detector s memory, voiding the new lamp s warranty. Warning: To prevent burn injuries, allow the lamp to cool for 30 minutes before removing it (15 minutes with the fan operating). The lamp and its housing become extremely hot during operation. Warning: To avoid eye injury from ultraviolet radiation exposure, power-off the detector before changing the lamp; wear eye protection that filters ultraviolet light; keep the lamp in the housing during operation. To remove the lamp: 1. Power-off the lamp. 2. Power-off the detector, and disconnect the power cable from the rear panel. 3. Allow the lamp to cool for 30 minutes. 4. Open the door. Warning: To prevent burn injuries, allow the lamp to cool for 30 minutes before removing it (15 minutes with the fan operating). The lamp and its housing become extremely hot during operation. 62 May 28, 2013, 715004023 Rev. A

Replacing the lamp 5. Detach the lamp s power connector from the detector. Alignment pin Captive screw Captive screw Lamp base Lamp power connector 6. Loosen the two captive screws in the lamp base. 7. Gently withdraw the lamp from the lamp housing. To install the lamp: Warning: To prevent shattering the glass, use care when disposing of the lamp. Lamp gas is under slight negative pressure. Waters suggests that you adequately cushion an old lamp by containing it in the packaging of its replacement before you dispose of it. Caution: To avoid adversely affecting detector operation, do not touch the glass bulb of the new lamp. If the bulb needs cleaning, gently rub it with ethanol and lens tissue. Do not use abrasive tissue. Do not apply excessive pressure. 1. Unpack the new lamp without touching the bulb. 2. Inspect the new lamp and lamp housing. May 28, 2013, 715004023 Rev. A 63

4 Maintaining the Detector 3. Position the lamp so that the cut-out on the lamp base plate is at the 1 o clock position, in line with the alignment pin on the lamp housing. Then gently push the lamp forward until it bottoms into position. 4. Ensure the lamp is flush with respect to the optics bench. 5. Tighten the two captive screws, and then reconnect the lamp s power connector. 6. Close the door. Caution: To prevent the lamp from binding and ensure that it is properly seated in the lamp housing, alternate between tightening the captive screws and pushing the lamp forward. 7. Power-on the detector, and then wait 1 hour for the lamp to warm before resuming operations. Tip: Cycling power to the detector initiates the verification procedures. 8. In the Instrument Console, select Maintain > Change Lamp. Change Lamp dialog box: 9. Click New Lamp. 64 May 28, 2013, 715004023 Rev. A

Replacing fuses New Lamp dialog box: 10. Type the serial number for the new lamp (see the label attached to the lamp connector wire), and then click OK. Replacing fuses The detector requires two 100 to 240 Vac, 50 to 60-Hz, F 3.15-A, 250-V (fast-blow), 5 20 mm (IEC) fuses. Suspect a fuse is open or otherwise defective when these events occur: the detector fails to power-on; the fan does not operate. Requirement: Replace both fuses, even when only one is open or otherwise defective. To replace the fuses: Warning: To avoid electric shock, power-off and unplug the ACQUITY PDA-TS detector from the wall outlet before examining the fuses. For continued protection against fire, replace fuses with those of the same type and rating only. 1. Power-off the detector, and disconnect the power cord from the power entry module. 2. Pinch the sides of the spring-loaded fuse holder, located above the power entry module on the rear panel of the detector. May 28, 2013, 715004023 Rev. A 65

4 Maintaining the Detector 3. With minimum pressure, withdraw the spring-loaded fuse holder. Fuses Power entry module TP02523 Fuse holder 4. Remove and discard the fuses. 5. Make sure that the new fuses are properly rated for your requirements, and then insert them into the holder and the holder into the power entry module, gently pushing until the assembly locks into position. 6. Reconnect the power cord to the power entry module. Cleaning the instrument s exterior Use a soft cloth dampened with water to clean the outside of the detector. 66 May 28, 2013, 715004023 Rev. A

A Safety Advisories Waters instruments and devices display hazard symbols that alert you to the hidden dangers associated with a product s operation and maintenance. The symbols also appear in product manuals where they accompany statements describing the hazards and advising how to avoid them. This appendix presents the safety symbols and statements that apply to all of the products that Waters offers. Contents: Topic Page Warning symbols... 68 Caution advisory... 71 Warnings that apply to all Waters instruments and devices... 72 Warnings that address the replacing of fuses... 77 Electrical and handling symbols... 79 May 28, 2013, 715004023 Rev. A 67

A Safety Advisories Warning symbols Warning symbols alert you to the risk of death, injury or seriously adverse physiological reactions associated with an instrument s use or misuse. Heed all warnings when you install, repair, or operate any Waters instrument or device. Waters accepts no liability in cases of injury or property damage resulting from the failure of individuals to comply with any safety precaution when installing, repairing, or operating any of its instruments or devices. The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device, or a component of an instrument or device. When one of these symbols appear in a manual s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid it. Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument s user documentation for important safety-related information before you use the instrument.) Warning: (Risk of burn injury from contacting hot surfaces.) Warning: (Risk of electric shock.) Warning: (Risk of fire.) Warning: (Risk of sharp-point puncture injury.) Warning: (Risk of hand crush injury.) Warning: (Risk of injury caused by moving machinery.) Warning: (Risk of exposure to ultraviolet radiation.) Warning: (Risk of contacting corrosive substances.) Warning: (Risk of exposure to a toxic substance.) Warning: (Risk of personal exposure to laser radiation.) 68 May 28, 2013, 715004023 Rev. A

Warning symbols Warning: (Risk of exposure to biological agents that can pose a serious health threat.) Warning: (Risk of tipping.) Warning: (Risk of explosion.) Warning: (Risk of eye injury.) Specific warnings The following warnings (both symbols and text) can appear in the user manuals of particular instruments and devices and on labels affixed to them or their component parts. Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized: Wear eye protection. Extinguish all nearby flames. Do not use tubing that is, or has been, stressed or kinked. Do not expose nonmetallic tubing to incompatible compounds like nitric or sulfuric acids. May 28, 2013, 715004023 Rev. A 69

A Safety Advisories Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans. Warning: To avoid infection with potentially infectious, human-sourced products, inactivated microorganisms, and other biological materials, assume that all biological fluids that you handle are infectious. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL). Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the biohazard safety representative for your organization regarding the proper use and handling of infectious substances. Biohazard and chemical hazard warning This warning applies to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials. Warning: To avoid personal contamination with biohazards, toxic materials, or corrosive materials, you must understand the hazards associated with their handling. Guidelines prescribing the proper use and handling of such materials appear in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Disposal of Chemicals. Observe Good Laboratory Practice (GLP) at all times, particularly when working with hazardous materials, and consult the safety representative for your organization regarding its protocols for handling such materials. 70 May 28, 2013, 715004023 Rev. A

Caution advisory Caution advisory Caution advisories appear where an instrument or device can be subject to use or misuse that can damage it or compromise a sample s integrity. The exclamation point symbol and its associated statement alert you to such risk. Caution: To avoid damaging the instrument s case, do not clean it with abrasives or solvents. May 28, 2013, 715004023 Rev. A 71

A Safety Advisories Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment. Important: Toute modification sur cette unité n ayant pas été expressément approuvée par l autorité responsable de la conformité à la réglementation peut annuler le droit de l utilisateur à exploiter l équipement. Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen. Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente. Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo. 注意 : 未經有關法規認證部門允許對本設備進行的改變或修改, 可能會使使用者喪失操作該設備的權利注意 : 未经有关法规认证部门明确允许对本设备进行的改变或改装, 可能会使使用者丧失操作该设备的合法性 주의 : 규정준수를책임지는당사자의명백한승인없이이장치를개조또는변경할경우, 이장치를운용할수있는사용자권한의효력을상실할수있습니다. 注意 : 規制機関から明確な承認を受けずに本装置の変更や改造を行うと本装置のユーザーとしての承認が無効になる可能性があります 72 May 28, 2013, 715004023 Rev. A

Warnings that apply to all Waters instruments and devices Warning: Use caution when working with any polymer tubing under pressure: Always wear eye protection when near pressurized polymer tubing. Extinguish all nearby flames. Do not use tubing that has been severely stressed or kinked. Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids. Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic tubing to swell, which greatly reduces the rupture pressure of the tubing. Attention: Manipulez les tubes en polymère sous pression avec precaution: Portez systématiquement des lunettes de protection lorsque vous vous trouvez à proximité de tubes en polymère pressurisés. Eteignez toute flamme se trouvant à proximité de l instrument. Evitez d'utiliser des tubes sévèrement déformés ou endommagés. Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF) ou de l'acide sulfurique ou nitrique concentré. Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture. Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht: In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille tragen. Alle offenen Flammen in der Nähe löschen. Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder konzentrierte Salpeter- oder Schwefelsäure verwenden. Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert. May 28, 2013, 715004023 Rev. A 73

A Safety Advisories Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. Spegnere tutte le fiamme vive nell'ambiente circostante. Non utilizzare tubi eccessivamente logorati o piegati. Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico o nitrico concentrati. Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di rottura dei tubi stessi. Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión: El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos de polímero sometidos a presión. Si hubiera alguna llama las proximidades. No se debe trabajar con tubos que se hayan doblado o sometido a altas presiones. Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano (THF) o ácidos nítrico o sulfúrico concentrados. Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos. 警告 : 當在有壓力的情況下使用聚合物管線時, 小心注意以下幾點當接近有壓力的聚合物管線時一定要戴防護眼鏡熄滅附近所有的火焰不要使用已經被壓癟或嚴重彎曲管線不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹, 大大降低管線的耐壓能力 74 May 28, 2013, 715004023 Rev. A

Warnings that apply to all Waters instruments and devices 警告 : 当有压力的情况下使用管线时, 小心注意以下几点 : 当接近有压力的聚合物管线时一定要戴防护眼镜熄灭附近所有的火焰不要使用已经被压瘪或严重弯曲的管线不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀, 大大降低管线的耐压能力 경고 : 가압폴리머튜브로작업할경우에는주의하십시오. 가압폴리머튜브근처에서는항상보호안경을착용하십시오. 근처의화기를모두끄십시오. 심하게변형되거나꼬인튜브는사용하지마십시오. 비금속 (Nonmetallic) 튜브를테트라히드로푸란 (Tetrahydrofuran: THF) 또는농축질산또는황산과함께사용하지마십시오. 염화메틸렌 (Methylene chloride) 및디메틸술폭시드 (Dimethyl sulfoxide) 는비금속튜브를부풀려튜브의파열압력을크게감소시킬수있으므로유의하십시오. 警告 : 圧力のかかったポリマーチューブを扱うときは注意してください加圧されたポリマーチューブの付近では必ず保護メガネを着用してください近くにある火を消してください著しく変形したまたは折れ曲がったチューブは使用しないでください非金属チューブにはテトラヒドロフラン (THF) や高濃度の硝酸または硫酸などを流さないでください塩化メチレンやジメチルスルホキシドは非金属チューブの膨張を引き起こす場合がありその場合チューブは極めて低い圧力で破裂します May 28, 2013, 715004023 Rev. A 75

A Safety Advisories Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L utilisateur doit être informé que si le matériel est utilisé d une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d être défectueuses. Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwenddung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren. Attenzione: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura. Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes. 警告 : 使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用, 那麼該設備所提供的保護將被消弱警告 : 使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用, 那么该设备所提供的保护将被削弱 경고 : 제조업체가명시하지않은방식으로장비를사용할경우장비가제공하는보호수단이제대로작동하지않을수있다는점을사용자에게반드시인식시켜야합니다. 警告 : ユーザーは製造元により指定されていない方法で機器を使用すると機器が提供している保証が無効になる可能性があることに注意して下さい 76 May 28, 2013, 715004023 Rev. A

Warnings that address the replacing of fuses Warnings that address the replacing of fuses The following warnings pertain to instruments equipped with user-replaceable fuses. If the fuse types and ratings appear on the instrument: Warning: To protect against fire, replace fuses with those of the type and rating printed on panels adjacent to instrument fuse covers. Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués sur le panneau à proximité du couvercle de la boite à fusible de l'instrument. Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert auf den Tafeln neben den Sicherungsabdeckungen des Geräts gedruckt sind. Attenzione: per garantire protezione contro gli incendi, sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate sui pannelli adiacenti alla copertura fusibili dello strumento. Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo y características impresos en los paneles adyacentes a las cubiertas de los fusibles del instrumento. 警告 : 為了避免火災, 更換保險絲時, 請使用與儀器保險絲蓋旁面板上所印刷之相同類型與規格的保險絲警告 : 为了避免火灾, 应更换与仪器保险丝盖旁边面板上印刷的类型和规格相同的保险丝 경고 : 화재의위험을막으려면기기퓨즈커버에가까운패널에인쇄된것과동일한타입및정격의제품으로퓨즈를교체하십시오. 警告 : 火災予防のためにヒューズ交換では機器ヒューズカバー脇のパネルに記載されているタイプおよび定格のヒューズをご使用ください May 28, 2013, 715004023 Rev. A 77

A Safety Advisories If the fuse types and ratings do not appear on the instrument: Warning: To protect against fire, replace fuses with those of the type and rating indicated in the Replacing fuses section of the Maintenance Procedures chapter. Attention: pour éviter tout risque d'incendie, remplacez toujours les fusibles par d'autres du type et de la puissance indiqués dans la rubrique "Remplacement des fusibles" du chapitre traitant des procédures de maintenance. Vorsicht: Zum Schutz gegen Feuer die Sicherungen nur mit Sicherungen ersetzen, deren Typ und Nennwert im Abschnitt "Sicherungen ersetzen" des Kapitels "Wartungsverfahren" angegeben sind. Attenzione: per garantire protezione contro gli incendi, sostituire i fusibili con altri dello stesso tipo aventi le caratteristiche indicate nel paragrafo "Sostituzione dei fusibili" del capitolo "Procedure di manutenzione". Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo y características indicados en la sección "Sustituir fusibles". 警告 : 為了避免火災, 更換保險絲時, 應使用維護步驟章節中更換保險絲所指定之相同類型與規格的保險絲警告 : 为了避免火灾, 应更换维护步骤一章的更换保险丝一节中介绍的相同类型和规格的保险丝 경고 : 화재의위험을막으려면유지관리절차단원의 퓨즈교체 절에설명된것과동일한타입및정격의제품으로퓨즈를교체하십시오. 警告 : 火災予防のためにヒューズ交換ではメンテナンス項目のヒューズの交換に記載されているタイプおよび定格のヒューズをご使用ください 78 May 28, 2013, 715004023 Rev. A

Electrical and handling symbols Electrical and handling symbols Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse May 28, 2013, 715004023 Rev. A 79