Puncturevine (Tribulus terrestris) Control in the South Okanagan. Ken Sapsford P.Ag. Lisa Scott M.Sc., R.P.Bio.

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Puncturevine (Tribulus terrestris) Control in the South Okanagan Ken Sapsford P.Ag. Lisa Scott M.Sc., R.P.Bio.

Puncturevine First recorded in California in 1903 First discovered in Canada in 1970 s In Canada, only occurs in Okanagan and Similkameen Valleys

Puncturevine Prefers dry, sandy or gravely soil. Distinguishing feature is it s spiny seed pod. Spines can damage feet of humans, wildlife and domestic animals and can injure the mouth, stomach or intestinal lining of animals when ingested. The spiny pods can also puncture bicycle tires.

Annual reproduces by seed only A single plant may produce up to 1 million seeds The seeds are found within the spiny seed pods Germination usually starts in late May in the Okanagan Germination will continue throughout the summer until frost Three weeks after plants appear, flowering begins and seed pods occur 1-2 weeks later. Puncturevine biology

Physical Control Puncturevine Control First plants found in an area should be removed before seedpods begin to form Plants should be disposed of by burning or burial at landfill if seed pods present Hoeing, shallow tillage or hand pulling of small patches Established patches plants must be removed annually Biological Control Two species of weevils were introduced in US in 1961 and when used in conjunction they have provided effective control Unable to successfully establish in the Okanagan Chemical Control Puncturevine is not listed on any known herbicide label in Canada Multiple applications (up to 5 per year) of glyphosate used in non crop areas.

Puncturevine trials 1. Pre-emerge Trial 2. Early Post-emerge Trial 3. Seeding and Mulching Trial

2015 site

2016 site

Pre-Emerge treatments 2015 1. Untreated Check 2. Alion (Indaziflam) Registered for Orchards and grapes 3. Prism (Rimsulfuron) Registered in some vegetable crops and in Washington State in grapes. 4. Devrinol (Napropamide) Registered in grapes, some berry crops and some vegetable crops. 5. Roundup (Glyphosate) 6. Chateau (Flumioxazin) Registered in Orchards and grapes and some vegetable crops 7. Authority (Sulfentrazone) Registered in grapes, apples, some berry crops and some vegetable crops. 8. Sandea (Halosulfuron) Registered on Apples and some vegetable crops

Pre-Emerge treatments 2015 1. Untreated Check 2. Alion (Indaziflam) Registered for Orchards and grapes 3. Prism (Rimsulfuron) Registered in some vegetable crops and in Washington State in grapes. 4. Devrinol (Napropamide) Registered in grapes, some berry crops and some vegetable crops. 5. Roundup (Glyphosate) 6. Chateau (Flumioxazin) Registered in Orchards and grapes and some vegetable crops 7. Authority (Sulfentrazone) Registered in grapes, apple, some berry crops and some vegetable crops. 8. Sandea (Halosulfuron) Registered on Apples and some vegetable crops

Pre-Emerge treatments 2016 1. Untreated Check 2. Alion (Indaziflam) Registered for Orchards and grapes 3. Prism (Rimsulfuron) Registered in some vegetable crops and in Washington State in grapes. 4. Chateau (Flumioxazin) Registered in Orchards and grapes and some vegetable crops 5. Sandea (Halosulfuron) Registered on Apples and some vegetable crops 6. Kerb (Propyzamide) Registered in Apples, Pears, Blueberries and some Vegetables 7. Prowl (Pendimethalin) Registered on some vegetable and field crops

Pre-Emerge treatments 2016 1. Untreated Check 2. Alion (Indaziflam) Registered for Orchards and grapes 3. Prism (Rimsulfuron) Registered in some vegetable crops and in Washington State in grapes. 4. Chateau (Flumioxazin) Registered in Orchards and grapes and some vegetable crops 5. Sandea (Halosulfuron) Registered on Apples and some vegetable crops 6. Kerb (Propyzamide) Registered in Apples, Pears, Blueberries and some Vegetables 7. Prowl (Pendimethalin) Registered on some vegetable and field crops

Treatment Application 5 km/h 40 psi 200 l/ha

All treatment were applied on May 21, 2015. Puncturevine was emerging at this time so Roundup Transorb was applied with all treatments A garden sprinkler was used to apply 1/4 inch (6 mm) of rainfall equivalent to the area 2 hours after application. Pre-emerge trial 2015

Pre-e Trial 2015 Puncturevine Count Plants/M2 350 300 250 200 150 100 50 0 18-Jun

Untreated Alion July 15 Prism Devrinol

Roundup Chateau July 15 Authority Sandea

Prism

100 80 Puncturevine control by the end of July 60 40 20 2015 2016 1 yr after app 0 Alion Prism Chateau Sandea

Alion plot 1 Year After Application Alion plot 2 Year After Application

Post-Emerge treatments 2015 Applied at 2 leaf stage 1. Untreated Check 2. Prisim 3. Ultim 4. Sandea 5. Ignite 6. Roundup 7. Vinegar

120 Post-E trial 2 leaf stage % Control 100 80 60 40 20 Prism Ultim Sandea Ignite Roundup Vinegar 0 8 DAA 20 DAA 35 DAA 48 DAA

Post emergent trial 2016 Products are approved in Invasive Species Management Plan Lontrel (clopyralid) did not control puncturevine. Clearview (aminopyralid + metsulfuron) controlled puncturevine 100% from 14 DAA through the entire growing season. Overdrive (dicamba + diflufenzapyr) controlled puncturevine 97% to 100% from 14 DAA through the entire growing season. Arsenal (imazapyr) controlled puncturevine 100% through the entire growing season. However Arsenal also controlled every other plant species that was growing in the plot and left bare ground for the entire growing season. by September the first plant species that was germinating was puncturevine.

Seeding and Mulching 1. Untreated Check 2. Grass seed mixture - seeded on May 21 st, raked in and 10 mm (0.4 in.) of water added after seeding 3. Mulching Orchard mulch applied June 3 rd at 7.5 cm (3 in.) depth

Seeding & Mulching Trial % Puncturevine Control 100 80 60 40 20 0 Untreated Seeded Mulched 09-Sep *Any control from seeding was due to the raking that removed puncturevine seedlings

Untreated Seeded Mulch

June 2016 1 year after application Mulch September 2015 Year of application

June 2017

Pre-emerge Trial Conclusions Alion, Prism, Chateau and Sandea appear to have suppressed or controlled puncturevine through part or all of the growing season. Post-emerge Trials Clearview and Overdrive provided season long control of puncturevine. Seeding & Mulch Trial Mulching with 3 inches of wood mulch significantly reduced puncturevine for 3 years.

2016 site Longspine Sandbur Prism applied preemergent was the only product that provided season long control of Longspine Sandbur

Funding and support Provided by Agriculture Environment Initiative Thank you for all the assistance from the OASISS staff and summer students

Questions Ken Sapsford P.Ag. Pesticide Specialist BC Ministry of Agriculture kenneth.sapsford@gov.bc.ca