PARTS GLOSSARY Basic Insufflation Package

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PARTS GLOSSARY Basic Insufflation Package www.promolife.com 888-742-3404 www.promolife.com/support/ www.promolife.com/videos/

EQUIPMENT, TUBING AND ACCESSORIES E O3Elite Single Ozone Generator Tubing with Check Valve G F Ozone Humidifier Ozone Earscope www.promolife.com 888-742-3404 sales@promolife.com Fayetteville, AR

TUBING AND ACCESSORIES A B Oxygen Tubing (5 ) C Silicone Tubing (Medium, 3 ) (This piece can be found with the Water Bubbler/Humidifier Combo) Silicone Tubing (Long, 5 ) D Silicone Tubing (Medium, 3 ) www.promolife.com 888-742-3404 sales@promolife.com Fayetteville, AR

TUBING AND ACCESSORIES H I Ozone Destruct System Ozone Syringe J K Multi-Chamber Insufflation Bag Catheter Female Two extra Luer Lock fittings are included. If you ever need to cut your tubing, these can help piece them back together. Male www.promolife.com 888-742-3404 sales@promolife.com Fayetteville, AR

Basic Insufflation Package Setup Guide 1. Hooking Up Your Oxygen 2. Insufflation Bag without Humidifier 3. Syringe Insufflation without Humidifier 4. Setting Up Your Humidifier 5. Humidified Ozone with a Bag 6. Humidified Ozone with a Syringe 7. Humidified Ozone with Earscope www.promolife.com 888-742-3404 www.promolife.com/support/ www.promolife.com/videos/ Download a digital copy of this guide at www.promolife.com/guides

Hook Up Your Oxygen 870 Medical Tank and Regulator 540 Industrial Tank and Regulator 1. Attach the oxygen regulator to your oxygen tank as shown. 2. Connect tubing (A) to the oxygen regulator. Attach the other end of tubing (A) to the Oxygen In port on the ozone generator. If you are using an oxygen concentrator, view a setup guide here: www.promolife.com/setup-flowmeter IMPORTANT instructions to lengthen the life of your regulator: 1. For 540 (industrial) regulator: To connect, it is important to insert the regulator into the valve connection of the cylinder at a perpendicular angle before tightening the nut. You can hand-tighten the nut initially, but the last ¼ to ½ turn needs to be wrench-tightened. For 870 (medical) regulator: To connect, simply use the two guide pegs to align and then tighten the T-handle of regulator. 2. Be sure the regulator is in the off position by rotating the knob counterclockwise to the 0 position. 3. For safety, be sure you are not directly in front of or behind the regulator when opening the cylinder valve until you are sure it is fully secured. 4. SLOWLY turn the cylinder valve on (counterclockwise) about one full turn. 5. If you can hear a hissing sound, there is a leak in the system. Turn the cylinder off and turn the regulator on to relieve any built-up pressure. Try tightening the regulator to the cylinder and opening the cylinder again. If this is an industrial (540) regulator, it is important to note that it does need to be wrench-tightened. If this does not help, shut the system down again and contact a service representative. NEVER attempt to repair a regulator or cylinder yourself. 6. Adjust the flow rate setting by truning the knob clockwise until the desired setting shows through the window on the regulator. 7. To turn off the system, close the cylinder valve, and wait for the oxygen to flow out of the regulator. At this point you should begin to see the gauge on the regulator decrease. To expedite this process, you can set the regulator to a whole number. Once the gauge has decreased, set the regulator dial back to 0. 8. NEVER try to remove the regulator unless the cylinder is off and pressure has been released. 9. If the cylinder pressure falls below 300 PSI, you should exchange the cylinder for a full one.

Insufflation Bag w/out Humidifier 1. Connect tubing (E) to the Ozone Out port of the Ozone Generator. 2. Connect the insufflation bag (J) to the end of tubing (E). Turn on oxygen and ozone to desired concentration to fill bag. 3. When bag is full, unscrew from tubing (E) and clamp the bag s tube with the clip. Turn off ozone and oxygen. 4. Attach catheter (K) to insufflation bag (J). 5. Unclamp tubing and begin your insufflation.

Syringe Insufflation without Humidifier 1. Connect tubing (E) to the Ozone Out port of the Ozone Generator. 2. Connect the syringe (I) to the end of tubing (E). Turn on oxygen and ozone to desired concentration to fill syringe. 3. When syringe is full, unscrew from tubing (E). Turn off ozone and oxygen. Tip syringe upright to prevent ozone from escaping. 4. Attach catheter (K) to syringe and begin insufflation. NOTE: It is important to lubricate the syringe prior to use. See the instructions that came with your syringe.

Setting Up Humidifier 1. Attach tubing (E) to the ozone out port on the ozone generator. Attach tubing (B) to the end of tubing (E). 2. Fill humidifier (F) to desired level of water. Connect the other end of tubing (B) to the glass straw that goes all the way into the humidifier. 3. Connect tubing (C) to the other straw of the humidifier. 4. Connect the end of tubing (C) to the bottom of the ozone destruct system (H). With this setup you can make ozonated water by simply turning on your oxygen and ozone. 5. Make sure the off on the destruct is positioned to the side. This will ensure the ozone is filtered until it is needed for use.

Humidified O3 With a Bag (You will need to set up your humidifier first, see instructions) After you run ozone through the water for 5 to 10 minutes, adjust ozone concentration to what is needed. 1. Remove the Luer lock fitting from the side arm of the destruct system (H). Connect the insufflation bag (J) in its place. 2. Turn the off toward the destruct system. This will allow the bag to fill. 3. Once bag is full, unscrew from the destruct system and clamp the tubing. Turn off to the side on your destruct (see photo 1). 4. Attach the catheter (K) to the insufflation bag (J). 5. When you are ready for your insufflation, unclamp tubing and begin your insufflation.

Humidified O3 with Syringe (You will need to set up your humidifier first, see instructions) After you run ozone through the water for 5 to 10 minutes, adjust ozone concentration to what is needed. 1. Remove the Luer lock fitting from the side arm of the destruct system (H). Connect the syringe (I) in its place. 2. Turn the Off on the destruct system toward the destruct system. This allows the syringe to begin filling. 3. Once syringe is full, unscrew from destruct system. Turn the Off on the destruct system back to the side (see photo 1). Attach the catheter (K) to the syringe and begin your insufflation. NOTE: It is important to lubricate the syringe prior to use. See the instructions that came with your syringe.

Humidified O3 with Earscope (You will need to hook up your humidifier first, see instructions) After you run ozone through the water for 5 to 10 minutes, adjust ozone concentration to what is needed. 1. Make sure the Luer lock fitting is attached to the side arm of the destruct system (H) Attach tubing set (D) to this fitting. 2. Attach the ozone earscope (G) to the end of tubing set (D). 3. To begin ear insufflation, turn the Off on the destruct system toward the destruct system and begin your insufflation. When you re done, turn the Off back to the side.