Mendoza NUTRIENT COMPOSITION OF Vitis vinifera SUPERIOR SEEDLESS AND RED GLOBE

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PIS SUPERFICIE CULTIVD CON VID Provincias EFFECT OF ROOTSTOCKS Superficie ha ON LEF Mendoza NUTRIENT COMPOSITION 160.704 OF Vitis vinifera San cvs. Juan SUPERIOR SEEDLESS 49.492 ND RED GLOBE nacionales La Rioja 8.519 Río Negro 2.636 Catamarca 2.584 Salta 2.296 María Beatriz Pugliese; Yanina Guzmán; Sergio Vega Mayor; Franco TOTL Pugliese; Pedro Gil; Sergio Mundaca; 228.575 Lisandro Bustos 92 % Sup. de viñedos Yuaider D., Castro D., Morales O., (Fuente: Escudero INV E. 2009. and Orosco G.

rea cultivated with vine in rgentina 218421 hectare 5,30% Table Grape 11496 hectare 81% San Juan Province 9283 hectare 12% Mendoza 7 % La Rioja-Río Negro-Neuquen- Catamarca-Salta Source: INV 2011

The most Important Varieties Superior Seedless 3274 ha Red Globe 2464 ha Flame Seedless 2166 ha

Destination of rgentina s table grape (80 000 ton) 45% 55% Export Market Local Market Source: Cámara de Productores y Exportadores de Frutas y Hortalizas de San Juan 2007-2013

Background Objective There is a lack of information in rgentina about the use of rootstocks for table The aim of this work was to evaluate the effect of rootstocks on the leaf grapes. nutrient/element composition of Vitis vinifera cvs. Superior Seedless and Red Globe, respectively. There is some information available on the effect of rootstock such as resistance to soil borne pests and diseases, vigor, production and fruit quality. There is not enough information available regarding interactions between the scion cultivar, the rootstock and the nutrient status of grapevines (Vitis vinifera L.) (Ibacache and Sierra 2009, Morales et al 2013). Several studies have shown that rootstocks affects the nutritional status of the scion cultivar (García et al 2001, Ibacache and Sierra 2009, Morales et al 2013)..

Materials and Methods Pocito- San Juan- rgentina The trellis system was a Parral (Pergola) (2,5 x 2,5 m) Drip irrigation was applied Clay loam soil The experimental design was a randomized complete block design, with four replicates Experimental unit: Two vines Treatments: Control Cereza Salt Creek 140Ru 101-14Mgt SO4 Harmony Freedom

Materials and Methods Caucete-San Juan-rgentina The trellis system was a Parral (Pergola) (3 x 3 m) Drip irrigation was applied Sandy loam soil The experimental design was a randomized complete block design, with four replicates Experimental unit: Two vines Treatments: Control Salt Creek 140Ru 1103P 101-14 Mgt SO4 Harmony Freedom

Nutritional Content Whole leaves (leaf blade and petiole) opposite the clusters were collected at veraison stage. The sample contained 80 whole leaves per treatment in each replicate. The total concentrations of N, P, K, Ca, Mg, Na, B, Fe, Mn, Zn, Cu were determined for each of the samples and were used as a reference for interpreting optimal and excessive nutritional levels Silva and Rodríguez (1995). ll leaf analysis were done at INT EE San Juan Laboratory. The study was conducted for five consecutive seasons from 2008/09 to 2012/13. Data were analyzed by NOV and the comparison of means were done by the Fisher LSD test, with a significance level of p 0,05.

Results

N % 2,40 C 1,80 B BC 1,20 Optimal level N 1,8-2,4 Clay loam soil timo 0,60 0,00 Cereza Salt Creek 140Ru 101 14 Control Freedon SO4 Harmony Total Nitrogen Content in leaf of Red Globe in Pocito, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Different letters mean significant differences in test LSD with p 0,05.

N % 2,60 2,31 2,02 B 1,73 1,44 1,16 0,87 0,58 0,29 0,00 Optimal level N 1,8-2,4 Sandy loam soil SO4 Salt Creek 1103P 140Ru Harmony Control Freedom Total Nitrogen Content in leaf of Superior Seedless in Caucete, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Different letters mean significant differences in test LSD with p 0,05.

P % 0,18 D 0,15 0,13 0,10 BC CD Optimal level P 0,2 Clay loam soil CD CD 0,08 0,05 0,03 0,00 Cereza 101 14 SO4 140Ru Harmony Control Salt Creek Freedon Total Phosphorus Content in leaf of Red Globe in Pocito, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Different letters mean significant differences in test LSD with p 0,05.

P % 0,210 C 0,180 BC 0,150 0,120 Optimal level P 0,2 Sandy loam soil 0,090 0,060 0,030 0,000 Control Freedom 1103P 140Ru Harmony SO4 Salt Creek Total Phosphorus Content in leaf of Superior Seedless in Caucete, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Different letters mean significant differences in test LSD with p 0,05.

K % 1,85 F 1,59 E 1,32 BC CD CD DE 1,06 0,79 Optimal level K 1,2 Clay loam soil 0,53 0,26 0,00 Control Cereza Salt Creek SO4 140Ru 101 14 Freedon Harmony Total Potassium Content in leaf of Red Globe in Pocito, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Different letters mean significant differences in test LSD with p 0,05.

K % 1,80 1,60 D 1,40 1,20 B BC BC BC C 1,00 0,80 Optimal level P 1,2 Sandy loam soil 0,60 0,40 0,20 0,00 Salt Creek SO4 1103P 140Ru Control Freedom Harmony Total Potassium Content in leaf of Superior Seedless in Caucete, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Different letters mean significant differences in test LSD with p 0,05.

SO4 Freedon Harmony 101 14 140Ru Salt Creek Cereza Control CD B BC DE BC E F B BC D Optimal level Mg 0,3 Optimal level Ca 1,5 CD CD E Mg % Ca % 0 0.5 1 1.5 2 2.5 3 3.5 4 Total Magnesium and Calcium Content in leaf of Red Globe in Pocito, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Different letters mean significant differences in test LSD with p 0,05.

SO4 Freedon Harmony Paulsen 1103 Ruggeri 140 Salt Creek Control C C C BC D B C Optimal level Mg 0,3 Optimal level Ca 1,5 C Mg % Ca % 0 0.5 1 1.5 2 2.5 3 Total Magnesium and Calcium Content in leaf of Superior Seedless in Caucete, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Different letters mean significant differences in test LSD with p 0,05.

Total Sodium and Boron Content in leaf in the cv Red Globe and Superior Seedless, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Red Globe Superior Seedless Rootstock %Na B ppm Rootstock % Na B ppm Control 0,19 c 179,08 a Control 0,17 b 57,44 a Cereza 0,17 c 173,50 a Salt Creek 0,14 a 74,00 bc Salt Creek 0,11 a 216,50 ab 140Ru 0,13 a 67,50 ab Ruggeri 140 0,11 a 226,75 b 1103P 0,14 a 78,88 bc 101 14 0,14 b 256,50 b Harmony 0,14 a 76,00 bc Harmony 0,14 b 246,50 b Freedom 0,14 a 63,94 ab Freedon 0,14 b 231,67 b SO 4 0,13 a 83,88 c SO 4 0,11 a 237,83 b p-valor 0,0001 0,0087 p-valor 0,0001 0,0337 Different letters mean significant differences in test LSD with p 0,05. Excess Na > 0,5 Optimal level B 40-60 Excess B > 300

Total iron, manganese, zinc and copper content in leaf of Red Globe in Pocito, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Rootstock Fe ppm Mn ppm Zn ppm Cu ppm Control 139,94 bcd 82,13 b 31,25 7,08 c Salt Creek 110,44 a 112,81 c 32,94 6,08 ab Cereza 149,25 cd 112,56 c 33,75 8,33 d Ruggeri 140 122,75 ab 107,19 c 32,00 5,67 a 101 14 141,75 bcd 81,13 b 33,94 6,33 abc Harmony 159,56 d 59,31 a 37,75 7, 17 c Freedon 153,81 cd 101,44 c 34,94 6, 75 bc SO 4 133,25 bc 99,25 c 31,67 8,63 d p-valor 0,001 0,001 0,070 0,001 Optimal level 50,00 30,00 30,00 4,00 Different letters mean significant differences in test LSD with p 0,05.

Total iron, manganese, zinc and copper Content in leaf of Superior Seedless in Pocito, San Juan, rgentina. Data are averages for the seasons 2008/09 to 2012/13. Portainjerto Fe ppm Mn ppm Zn ppm Cu ppm Control 144,44 80,50 c 36,69 9,88 Salt Creek 128,63 65,75 bc 39,13 8,63 140Ru 139,19 59,00 b 36,13 9,38 1103P 133,06 42,88 a 35,56 8,75 Harmony 153,38 59,17 b 36,19 10,75 Freedon 150,38 40,44 a 34,94 8,63 SO 4 132,50 66,50 bc 37,25 8,50 p-valor 0,5096 0,0005 0,9120 0,6700 Optimal level 50 30 30 4,00 Different letters mean significant differences in test LSD with p 0,05.

Salt Creek Harmony Highest levels P Highest levels K Cereza Highest levels Mg and Ca SO4 Suboptimal Mg levels Vitis vinifera L. Lowest levels B merican Rootstocks Lowest levels Na

Significance of Study This study demonstrated the effect of rootstocks on the leaf nutrient composition of two table grape scion cultivars. This study showed that merican rootstocks resulted in the lowest scion Na leaf content. Therefore, it could be said that for soil with high sodium content, these particular rootstocks could be used. Cereza rootstock showed the highest levels of Ca and Mg and also exhibited highest production and grape quality (data not presented). Vitis vinifera L., Red Globe and Superior Seedless on Own root and Cereza obtained the lowest B leaf content, which could be potentially of practical value for agro climatic conditions where high B levels are present in both water and soil. Further studies are required to understand the influence of the rootstock on mineral absorption.

Effect of different rootstocks on variables of yield, quality, nutritional levels, vigor in different soil and salt types INT EE San Juan: María Beatriz Pugliese, Sergio Vega Mayor, Mario Liotta, Daniela Pacheco, Pedro Gil, Sergio Mundaca, Lisandro Bustos, Pablo Esteso, Monica Riuz, Maximiliano Battistella UNSJ-INT: Yanina Guzmán

PIS SUPERFICIE CULTIVD CON VID THNK YOU!!! Provincias Superficie ha Mendoza 160.704 San Juan MUCHS GRCIS!!! 49.492 La Rioja 8.519 Río Negro 2.636 Catamarca 2.584 Salta 2.296 TOTL pugliese.maria@inta.gob.ar 228.575 92 % Sup. de viñedos nacionales (Fuente: INV 2009. EE San Juan