Citrus Research and Development Foundation, Inc. Funded Projects to Aid Growers in the HLB Battle Florida Citrus Growers Institute April 7, 2015 Harold Browning, Chief Operations Officer 700 Experiment April 27, 2015Station Road Lake Alfred FL 33850 863-956-8817 citrusrdf.org 1
TIME Progressive Biological Impacts of HLB: 2005-2014 Inoculation into Leaves by Psyllid Systemic Movement in Vascular Tissue Mottling of Leaves (starch) Debilitation of Root Systems Decline of Limbs, Thinning of Canopies Small, Off-flavored Fruit Pre-harvest Fruit Drop Increase Overall Tree Decline Increasingly Compounded by Other Stresses Individual Grove Failure April 27, 2015 2
Current IPM Practices for Arthropods Asian citrus psyllid control Organophosphates, carbamates, pyrethroids, neonicotinoids, IGRs Citrus Health Management Areas established and working Biological control with Tamarixia radiata Bee safety Management of insecticides in presence of bees. Citrus leafminer, Diaprepes root weevil, mites, other pests Integrate with ACP Management Decisions April 27, 2015 3
Horticultural Practices to Complement HLB Disease Management Chemical and Thermal Therapy to Reduce Bacteria in Trees Naturally Occurring Microbial Products Plant Growth Regulators - Fruit Drop due to HLB Integrated Root Health Practices Field Delivery of Tolerant or Resistant Citrus Germplasm Evaluation of Advanced Citrus Production Systems Integrating All Tools in New Plantings Inoculum Removal Accumulation of infected trees 4/27/2015 www.citrusrdf.org 4
Approaches to Battle HLB Preventing spread (new plantings and resets) Reducing Disease in Infected Trees Sustaining/Improving Tree Health Fruit Drop and Fruit Quality Reducing Impacts of other Diseases and Stresses April 27, 2015 5
Approaches to Battle HLB PREVENTING SPREAD Research Delivery CRDF NIFA CRDF MAC ACP Movement 814,674 211,681 ACP Management & CHMAs Rogers Pelz-Stelinski Stansly 4,100,103 4,500,000 1,426,265 2,998,000 New Plantings Systems 936,705 615,000 Tolerance in Rootstocks and Scions Grosser, Boman 1,424,649 4,916,042 1,000,003 April 27, 2015 6
Support for New Rootstocks New commercial scale plantings of candidate tolerant rootstocks UF and USDA Releases Nursery Licensing Support for Overcoming Propagation Challenges Phase I Field Trial Plantings Phase II under Planning Field Days and Communication April 27, 2015 7
Approaches to Battle HLB REDUCING DISEASE IN TREES Research Delivery CRDF NIFA CRDF MAC Bactericides Slinski 1,155,142 6,700,000 1,753,245 1,326,000 Thermal Therapy Ehsani 3,500,000 927,455 1,266,000 Inoculum Removal 1,000,000 April 27, 2015 8
Approaches to Battle HLB SUSTAINING TREE HEALTH Research Delivery CRDF NIFA CRDF MAC Nutrition Schumann 407,500 Soil/Water Conditions & Treatments 477,576 385,193 173,000 Compost, Microbe Products Morgan 187,296 782,078 216,000 Integrating Approaches to Health 1,543,000 April 27, 2015 9
Approaches to Battle HLB FRUIT DROP AND FRUIT QUALITY Research Delivery CRDF NIFA CRDF MAC Influence of PGRs on Infected Trees 470,849 814,144 Impact of HLB on Fruit Quality 110,000 April 27, 2015 10
Approaches to Battle HLB REDUCE IMPACT OF OTHER DISEASES Research Delivery CRDF NIFA CRDF MAC Citrus Canker Graham 3,043,544 834,133 Citrus Black Spot 909,302 Phytophthora 336,714 Diaprepes Root Weevil 80,000 134,500 Citrus Blight 400,000 April 27, 2015 11
Ultimate HLB Management Asian Citrus Psyllid Population (Vector) Monitoring Attract/Repel Pesticides CHMAs Biological Control Defective ACP (NIFA SCRI) CLas Bacterial Inoculum (Pathogen) Better Detection Tree Removal Bactericides Thermal Therapy Tree Defense Other Therapy Tree Susceptibility To HLB and Injury (Host) Optimal Nutrition/Irrigation Increase Plant Defense Tolerant Rootstocks Breeding for Resistance Root Health Impacts Accelerate Production Replant Citrus Trees X X Low Low Low Transmission Infection Tree Injury = Reduced HLB Disease Severity 4/27/2015 www.citrusrdf.org 12
Citrus Research and Development Foundation, Inc. Thank you! CRDF is proud to provide support to the Florida citrus industry April 27, 2015 13
Project Profile by Topics CRDF Research CRDF Research Category Projects Ending < 7/01/15 Projects Continuing > 7/01/15 1. Consequences of HLB infection 2. CLas culture, genomics, molecular 3. Citrus response to infection 4. HLB pathogen/disease detection 5. HLB epidemiology and mitigation 6. ACP monitoring and behavior 7. ACP chemical, biological mgmt 8. ACP trapping and repelling plants 9. Citrus genomics and transcriptomics 10. Conventional citrus resistance breeding 11. Transgenic/ vector mediated resistance 12. Model systems, inc. screening Non HLB Pest and Disease Research # Projects Total Budget # Projects Total Budget 0 1 $ 237,500 7 $ 1,952,070 4 $ 1,680,137 4 $ 304,090 2 $ 496,182 3 $ 583,581 0 11 $ 2,459,788 8 $ 2,504,017 4 $ 628,470 1 $ 577,703 7 $ 1,719,149 4 $ 1,127,681 0 0 1 $ 458,000 1 $ 240,000 7 $ 2,317,621 4 $ 2,052,136 11 $ 2,832,556 6 $ 3,026,921 2 $ 296,384 3 $ 1,318,803 7 $ 1,356,198 9 $ 3,162,545 Total Research Projects 64 $ 14,907,907 43 $ 16,423,625 April 27, 2015 14
Project Profile by Topics CRDF Delivery CRDF Commercial Product Delivery Projects Ending < 7/01/15 Projects Continuing > 7/01/15 Program Topic # Projects Total Budget # Projects Total Budget Antimicrobial therapy Naturally Occurring Microbes Thermal Therapy Plant Growth Regulators ACP Management Tolerant Rootstock Plantings Psyllid Shield (RNAi) HLB Escapes Non-HLB Pests and Diseases Total CPD Projects 9 $ 2,567,858 4 $ 506,587 0 4 $ 468,386 2 $ 467,177 1 $ 105,782 5 $ 175,717 1 $ 132,660 3 $ 269,720 0 3 $ 548,088 1 $ 333,774 1 $ 487,383 1 $ 113,523 1 $ 176,000 0 1 $ 803,126 3 $ 213,333 25 $ 5,495,069 15 $ 1,874,045 April 27, 2015 15