Details of Application Application Number 2007/275 Variety Name MAC03 Genus Species Zoysia macrantha Common Name Prickly Couch

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Details of Application Application Number 2007/275 Variety Name MAC03 Genus Species Zoysia macrantha Common Name Prickly Couch Synonym Nara Accepted Date 30 Nov 2007 Applicant Ozbreed Pty Ltd, Clarendon, NSW Agent N/A Qualified Person Ian Paananen Details of Comparative Trial Location Descriptor Cynodon (Cynodon dactylon x C. transvaalensis) PBR CYNO Period Oct 2007 Feb 2008. Conditions Trial conducted in open beds, plants propagated from cuttings, planted into 200mm pots filled with soilless potting mix, nutrition maintained with slow release and liquid fertilisers, irrigation by overhead watering, pest and disease treatments not required. Plants trimmed 10 weeks before assessment. Trial Design Thirty pots of each variety arranged in a completely randomised design. Measurements From ten plants at random. RHS Chart - edition 2001. Origin and Breeding Open pollination followed by seedling selection: seed parent Zoysia macrantha. 2000-2005: open pollination of Zoysia macrantha selections originally collected from NSW coastal areas including Central Coast and Wollongong. Each year, selections were made based on speed of growth and overall turf habit (density, ground coverage) and these were then the parents for the following year s selections. Slower varieties were discarded. The result was greatly improved growth rates more suited to lawn use. 2006-2007: the final selection was made from the parent selection T11. This selection was considered to have a good turf habit, a broader leaf width, fast growth rate and good salt tolerance. The seed parent is characterised by narrow leaf width, medium growth vigour and medium salt tolerance. Selection took place in Clarendon, NSW in 2006. Selection criteria: broader leaf width, strong growth vigour, dense growth habit and strong salt tolerance. Propagation: vegetative, division is found to be uniform and stable. Breeder: Todd Layt, All work was carried out at Choice of Comparators Characteristics used for grouping varieties to identify the most similar Variety of Common Knowledge Organ/Plant Part State of Expression in Group of Varieties Stolon internode length medium Inflorescence length of peduncle medium Inflorescence anthers present Leaf colour yellow green

Most Similar Varieties of Common Knowledge identified (VCK) Name Comments T11 parent variety selected by Ozbreed. Terrigal Wollongong Variety Description and Distinctness - Characteristics which distinguish the candidate from one or more of the comparators are marked with a tick. MAC03 T11 Terrigal Wollongong Plant: habit stoloniferous stoloniferous stoloniferous stoloniferous Stolon: internode length medium medium medium medium Stolon: internode broad medium medium medium thickness Stolon: colour when exposed to sunlight 187A 187A 187A 187A Leaf blade: shape ligulate ligulate ligulate ligulate Leaf blade: length long medium to long medium medium Leaf blade: width broad medium medium medium Leaf blade: colour 146B 146A 146A 146A Inflorescence: length of medium medium medium medium peduncle Leaf blade: apex narrow acute narrow acute narrow acute narrow acute Inflorescence: anthers present present present present Characteristics Additional to the Descriptor/TG MAC03 T11 Terrigal Wollongong Plant: vigour strong to very strong medium to strong medium weak to medium Stolon: length long to very long medium medium short to medium Stolon: diameter of node medium to broad narrow to mediummedium medium Leaf blade: attitude horizontal to horizontal to horizontal to horizontal to semi-erect semi-erect semi-erect semi-erect Leaf blade: profile in cross-section flat flat flat flat Glume: predominant colour (RHS) N186C N186C N186C N186C Inflorescence: length of spike medium to long medium to long medium medium Peduncle: colour (RHS) 147B 147B 147B 147B From left to right: MAC03 T11 Terrigal Ecotype Wollongong Ecotype Image from Plant Breeders Rights Database online: http://pbr.ipaustralia.plantbreeders.gov.au/image_detail.cfm?aid=2547966

Details of Application Application Number 2007/275 Variety Name MAC03 Genus Species Zoysia macrantha Common Name Prickly Couch Synonym Nara Accepted Date 30 Nov 2007 Applicant Ozbreed Pty Ltd, Clarendon, NSW Agent N/A Qualified Person Ian Paananen Details of Comparative Trial Location Descriptor Cynodon (Cynodon dactylon x C. transvaalensis) PBR CYNO Period Oct 2007 Feb 2008. Conditions Trial conducted in open beds, plants propagated from cuttings, planted into 200mm pots filled with soilless potting mix, nutrition maintained with slow release and liquid fertilisers, irrigation by overhead watering, pest and disease treatments not required. Plants trimmed 10 weeks before assessment. Trial Design Thirty pots of each variety arranged in a completely randomised design. Measurements From ten plants at random. RHS Chart - edition 2001. Origin and Breeding Open pollination followed by seedling selection: seed parent Zoysia macrantha. 2000-2005: open pollination of Zoysia macrantha selections originally collected from NSW coastal areas including Central Coast and Wollongong. Each year, selections were made based on speed of growth and overall turf habit (density, ground coverage) and these were then the parents for the following year s selections. Slower varieties were discarded. The result was greatly improved growth rates more suited to lawn use. 2006-2007: the final selection was made from the parent selection T11. This selection was considered to have a good turf habit, a broader leaf width, fast growth rate and good salt tolerance. The seed parent is characterised by narrow leaf width, medium growth vigour and medium salt tolerance. Selection took place in Clarendon, NSW in 2006. Selection criteria: broader leaf width, strong growth vigour, dense growth habit and strong salt tolerance. Propagation: vegetative, division is found to be uniform and stable. Breeder: Todd Layt, All work was carried out at Choice of Comparators Characteristics used for grouping varieties to identify the most similar Variety of Common Knowledge Organ/Plant Part State of Expression in Group of Varieties Stolon internode length medium Inflorescence length of peduncle medium Inflorescence anthers present Leaf colour yellow green

Statistical Table MAC03 T11 Terrigal Wollongong Stolon: length (cm) Mean 40.40 20.70 18.60 15.00 Std. Deviation 6.50 3.50 4.00 3.00 LSD/sig 7.06 P 0.01 P 0.01 P 0.01 Stolon: length of internode (mm) Mean 28.80 27.70 30.10 26.00 Std. Deviation 7.20 6.80 6.50 5.60 LSD/sig 7.51 ns ns ns Stolon: diameter of internode (mm) Mean 1.95 1.34 1.44 1.40 Std. Deviation 0.40 0.20 0.20 0.20 LSD/sig 0.32 P 0.01 P 0.01 P 0.01 Stolon: diameter of node (mm) Mean 2.60 2.04 2.23 2.40 Std. Deviation 0.60 0.40 0.30 0.30 LSD/sig 0.48 P 0.01 ns ns Leaf blade: length (mm) Mean 96.30 63.90 43.70 53.20 Std. Deviation 11.90 23.30 13.40 13.40 LSD/sig 17.72 P 0.01 P 0.01 P 0.01 Leaf blade: width (mm) Mean 3.80 2.92 2.71 3.10 Std. Deviation 0.70 0.20 0.50 0.30 LSD/sig 0.49 P 0.01 P 0.01 P 0.01 Inflorescence: length of spike (mm) Mean 52.20 49.90 45.50 40.10 Std. Deviation 3.90 4.90 4.70 9.60 LSD/sig 7.10 ns ns P 0.01 Peduncle: length (mm) Mean 122.60 136.90 102.90 97.20 Std. Deviation 21.50 22.90 25.00 24.60 LSD/sig 26.73 ns ns ns Prior Applications and Sales Nil. Description: Ian Paananen, Crop & Nursery Services, Central Coast, NSW.

Details of Application Application Number 2007/275 Variety Name MAC03 Genus Species Zoysia macrantha Common Name Prickly Couch Synonym Nara Accepted Date 30 Nov 2007 Applicant Ozbreed Pty Ltd, Clarendon, NSW Agent N/A Qualified Person Ian Paananen Details of Comparative Trial Location Descriptor Cynodon (Cynodon dactylon x C. transvaalensis) PBR CYNO Period Oct 2007 Feb 2008. Conditions Trial conducted in open beds, plants propagated from cuttings, planted into 200mm pots filled with soilless potting mix, nutrition maintained with slow release and liquid fertilisers, irrigation by overhead watering, pest and disease treatments not required. Plants trimmed 10 weeks before assessment. Trial Design Thirty pots of each variety arranged in a completely randomised design. Measurements From ten plants at random. RHS Chart - edition 2001. Origin and Breeding Open pollination followed by seedling selection: seed parent Zoysia macrantha. 2000-2005: open pollination of Zoysia macrantha selections originally collected from NSW coastal areas including Central Coast and Wollongong. Each year, selections were made based on speed of growth and overall turf habit (density, ground coverage) and these were then the parents for the following year s selections. Slower varieties were discarded. The result was greatly improved growth rates more suited to lawn use. 2006-2007: the final selection was made from the parent selection T11. This selection was considered to have a good turf habit, a broader leaf width, fast growth rate and good salt tolerance. The seed parent is characterised by narrow leaf width, medium growth vigour and medium salt tolerance. Selection took place in Clarendon, NSW in 2006. Selection criteria: broader leaf width, strong growth vigour, dense growth habit and strong salt tolerance. Propagation: vegetative, division is found to be uniform and stable. Breeder: Todd Layt, All work was carried out at Choice of Comparators Characteristics used for grouping varieties to identify the most similar Variety of Common Knowledge Organ/Plant Part State of Expression in Group of Varieties Stolon internode length medium Inflorescence length of peduncle medium Inflorescence anthers present Leaf colour yellow green

Statistical Table MAC03 T11 Terrigal Wollongong Stolon: length (cm) Mean 40.40 20.70 18.60 15.00 Std. Deviation 6.50 3.50 4.00 3.00 LSD/sig 7.06 P 0.01 P 0.01 P 0.01 Stolon: length of internode (mm) Mean 28.80 27.70 30.10 26.00 Std. Deviation 7.20 6.80 6.50 5.60 LSD/sig 7.51 ns ns ns Stolon: diameter of internode (mm) Mean 1.95 1.34 1.44 1.40 Std. Deviation 0.40 0.20 0.20 0.20 LSD/sig 0.32 P 0.01 P 0.01 P 0.01 Stolon: diameter of node (mm) Mean 2.60 2.04 2.23 2.40 Std. Deviation 0.60 0.40 0.30 0.30 LSD/sig 0.48 P 0.01 ns ns Leaf blade: length (mm) Mean 96.30 63.90 43.70 53.20 Std. Deviation 11.90 23.30 13.40 13.40 LSD/sig 17.72 P 0.01 P 0.01 P 0.01 Leaf blade: width (mm) Mean 3.80 2.92 2.71 3.10 Std. Deviation 0.70 0.20 0.50 0.30 LSD/sig 0.49 P 0.01 P 0.01 P 0.01 Inflorescence: length of spike (mm) Mean 52.20 49.90 45.50 40.10 Std. Deviation 3.90 4.90 4.70 9.60 LSD/sig 7.10 ns ns P 0.01 Peduncle: length (mm) Mean 122.60 136.90 102.90 97.20 Std. Deviation 21.50 22.90 25.00 24.60 LSD/sig 26.73 ns ns ns Prior Applications and Sales Nil. Description: Ian Paananen, Crop & Nursery Services, Central Coast, NSW.

Statistical Table MAC03 T11 Terrigal Wollongong Stolon: length (cm) Mean 40.40 20.70 18.60 15.00 Std. Deviation 6.50 3.50 4.00 3.00 LSD/sig 7.06 P 0.01 P 0.01 P 0.01 Stolon: length of internode (mm) Mean 28.80 27.70 30.10 26.00 Std. Deviation 7.20 6.80 6.50 5.60 LSD/sig 7.51 ns ns ns Stolon: diameter of internode (mm) Mean 1.95 1.34 1.44 1.40 Std. Deviation 0.40 0.20 0.20 0.20 LSD/sig 0.32 P 0.01 P 0.01 P 0.01 Stolon: diameter of node (mm) Mean 2.60 2.04 2.23 2.40 Std. Deviation 0.60 0.40 0.30 0.30 LSD/sig 0.48 P 0.01 ns ns Leaf blade: length (mm) Mean 96.30 63.90 43.70 53.20 Std. Deviation 11.90 23.30 13.40 13.40 LSD/sig 17.72 P 0.01 P 0.01 P 0.01 Leaf blade: width (mm) Mean 3.80 2.92 2.71 3.10 Std. Deviation 0.70 0.20 0.50 0.30 LSD/sig 0.49 P 0.01 P 0.01 P 0.01 Inflorescence: length of spike (mm) Mean 52.20 49.90 45.50 40.10 Std. Deviation 3.90 4.90 4.70 9.60 LSD/sig 7.10 ns ns P 0.01 Peduncle: length (mm) Mean 122.60 136.90 102.90 97.20 Std. Deviation 21.50 22.90 25.00 24.60 LSD/sig 26.73 ns ns ns Prior Applications and Sales Nil. Description: Ian Paananen, Crop & Nursery Services, Central Coast, NSW.

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