Plant Propagation Protocol for Triteleia hyacinthina TAXONOMY GENERAL INFORMATION

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Plant Propagation Protocol for Triteleia hyacinthina ESRM 412 Native Plant Production Protocol URL: https://courses.washington.edu/esrm412/protocols/trhy3.pdf TAXONOMY Plant Family Scientific Name Liliaceae Common Name Lily Species Scientific Name Scientific Name Triteleia hyacinthina (Lindl.) Greene 8 Varieties Sub-species Cultivar Common Synonym(s) Brodiaea dissimulata M. Peck Hesperoscordum hyacinthinum Lindl. Brodiaea hyacinthina (Lindl.) Baker Brodiaea hyacinthina (Lindl.) Baker var. lactea Baker 8 Common Name(s) Wild hyacinth, White Brodiaea 9, Fool s onion 6 Species Code (as per TRHY3 8 USDA Plants database) GENERAL INFORMATION

Geographical range Ecological distribution) Open, grassy areas at low elevations to mountain meadows 6 Grasslands, close-cone pine forests, foothill woodland, vernally wet meadows 9 Wet prairies 10 Thin-spoiled swales that are damp in winter and spring but dry out during summer 7

Grassy, often rocky, open flats to midmontane meadows, common in sagebrush desert east of the Cascades from Chelan County, WA, east to ID and south through northern NV 3 Climate and elevation range Local habitat and abundance Seasonally flooded herbaceous balds and seasonally wet depressions on bedrock, coastal bluffs of the Puget Trough 2 Zero to 2000 m 9 Deschampsia caespitosa, Danthonia californica, Camassia quamash, Grindelia nana, Carex densa, Carex unilateralis 10 Antennaria rosea, Allium cernuum,allium acuminatum, Eriophyllum lanatum, Achillea millefolium, Fragaria chiloensis 5 Plant strategy type / successional stage Plant characteristics Prunella vulgaris, Leptosiphon bicolor, Festuca idahoensis ssp. Roemeri, Mimulus guttatus, Zigadenus venenosus, Madia gracilis 2 Shade intolerant 2 Perennial forb White bell-shaped flowers, sometimes with a pale blue tint, and bluish green petal midveins, 1-1.5 cm long and carried in an upright cluster atop 3-5 bracts 6 Distinguishabable from other white-flowered Triteleia species by short pedicels and by yellow, rather than lavender, anthers 7 20-24 tall with one or two basal leaves to 16 and wiry stems to 28 5 PROPAGATION DETAILS Ecotype: The Nature Conservancy s Dye Creek and Vina Plains Preserves, Tehama county, CA 4 Propagation Goal: Bulbs 4 Propagation Method: Seed 4 Product Type: Container (plug) 4 Stock Type: Potted nursery stock 4 Time to Grow: 7 months 4 Target Specifications: First year bulb, 3-8 mm in diameter 4 Propagule Collection: Whole fruits can be collected from heads immediately prior to or after ripening. Seed may be collected from May-July, with seeds being retained on the heads well into summer, depending on the year. Seed may be shaken from capsules that have split, but unopened capsules may need to be mechanically split (although dry heat or repeated drying and cooling may cause the capsules to open). 4

Propagule Processing/Propagule Characteristics Pre-Planting Propagule Treatments Seed density is approximately 300-450 seeds per gram (136,000-205,000 per pound), depending on population, year and cleanliness of seed 4 Large numbers of dry fruits may be shattered rapidly by very brief blending (dry in a blender). After blending, seed may be cleaned by sifting or blowing off chaff (e.g. a blow dryer). 4 Dormancy treatments were variable among propagators. The University of California-Chico reported that no dormancy treatments were needed, although clean dry seed was placed in dry, cold storage following collection and prior to sowing. 4 The MsK Nursery at the Kruckeberg Botanic Garden in Shoreline, WA reported a stratification period of one month under refrigeration, 1 perhaps reflecting the change in provenance at more northerly latitudes. Growing Area Preparation / Annual Practices for Perennial Crops Seed was sown directly into 1.5 deep flats containing a 1:1:1:2 sand:pumice:peat moss:fir bark potting mixture. Flats were placed in an outdoor cold frame from late fall through spring. Seedlings were transplanted into various sized pots ranging from D-pots to 3x4 plastic containers using the same potting mixture. 4 It was not indicated which container type worked best. Seed sown into 4 bulb pots. Typical seeding mix containing peat moss was found to hold too much moisture. Cut seed sowing mix by ½ by adding sand 1 Establishment Phase Details: Length of Establishment Phase Active Growth Phase Plant seeds in six-inch (or bigger) pots because the corms will pull themselves down to the depth they require. Plant about 100 seeds per pot and place them on top of the soil and sprinkle a little soil over them and 1/4 inch gravel on top. Set in partial shade to prevent drying quickly either in a greenhouse or outside. 9 Germination was observed within two weeks. Germination rate was 96% for seed cold, moist stratified in vermiculite at approximately 44 F. For seeds own in outdoor coldframes, germination was 95%. Small bulbs (2-6 mm in diameter will be produced within 6-8 months. 4 Transplantable sprouts were established within 3-4 weeks. 4 Active growth was observed from the onset of autumn rains until drying-down (die-back and dormancy) occurred in late spring/early summer. The length of the active growth phase can be somewhat controlled with irrigation, but this species requires summer dormancy. 4 Can be placed in a cold frame to keep off excess moisture 1

Length of Active Growth Phase Hardening Phase Length of Hardening Phase Harvesting, Storage and Shipping Length of Storage 3-5 months 4 6-8 months (late fall-early summer) 4 Hardening is not necessary as the active growth phase begins with the onset of fall rains and plants senesce toward the end of spring/beginning of summer. 4 Individuals go dormant following spring-summer dry down and die back to the root. Dormant individuals were placed in dry storage at 60-70 F 4 2 years 1 Guidelines for Outplanting / Performance on Typical Sites Other Comments References: Outplanted area may require regular weeding and the use of a very weak solution of fertilizer twice during active growth during the spring. Corms can be kept from overcrowding by thinning them every three years by picking off the cormlets and replanting elsewhere 9 Naturalizes well in warm, sunny locations or semi-shade under deciduous trees. Needs a light sandy soil mixture with good drainage. Corms will produce small bulbs every few years that can be separated and replanted. Takes 3-5 years to produce mature, flowering plants 1,5 INFORMATION SOURCES 1. Beaudry, Roseanne. MsK Nursery, Kruckeberg Botanic Garden. (personal communication 2 May, 2014) 2. Chappell CB. 2006. Plant associations of balds and bluffs of western Washington. Natural Heritage Report 2006-02. Washington State Dept. of Nat. Res. (accessed 11 April 2014). Available from: URL: http://www1.dnr.wa.gov//nhp/refdesk/communities/pdf_balds_veg.pd f 3. Hitchcock CL and Cronquist A. 1973. Flora of the Pacific Northwest. Seattle: University of Washington Press 4. Hunt JW, Boul RD, Brown MR, Koenig DA, Leigh M, Pushnik JC. 2006. Propagation protocol for production of container Triteleia hyacinthina bulbs (potted nursery stock): University of California-

Chico, Chico, CA. In: Native Plant Network. URL: http://www.nativeplantnetwork.org (accessed 11 April 2014). Moscow (ID): University of Idaho, College of Natural Resources, Forest Research Nursery 5. Pettinger A and Costanzo B. 1996. Native Plants in the Coastal Garden. North Vancouver (BC): Whitecap Books. 6. Pojar J and MacKinnon A. 1994. Plants of the Pacific Northwest Coast. Vancouver: Lone Pine Publishing. 7. Sanderson P and McGary J. 2001 In: McGary J (editor) Bulbs of North America. Portland (OR):Timber Press. 8. USDA Natural Resources Conservation Service Plants Database. [Internet] URL: http;//www.plants.usda.gov/core/profile?symbol=trhy3 (accessed 11 April 2014) 9. USDA NRCS National Plant Data Center & East Bay Regional Parks Botanic Garden. 2001. Anderson MK and Roderick W (eds) Plant guide white Brodiaea Triteleia hyacinthina (Lindl.): [Internet] URL: http://www.plants.usda.gov/plantguide/pdf/cs_trhy3.pdf (accessed 11 April 2014). 10. Wetlands of conservation concern [Internet]. ND. (accessed 16 May, 2014). Available from http://www.oregon.gov/dsl/permits/docs/wetland_cons_concern.p df Other Sources Consulted Protocol Author Date Protocol Created or Updated Rose R, Chachulski CEC and Haase DL. 1998. Propagation of Pacific northwest native plants. Corvallis (OR): Oregon State University Press Mark Thompson 05/17/2014