IR-4 Ornamental Horticulture Program Research Report Cover Sheet

20150415c.pdf IR-4 Ornamental Horticulture Program Research Report Cover Sheet Researcher(s) Nora J Catlin Trial: 2014 NY Affiliation: Cornell Cooperative Extension of Suffol 423 Griffing Avenue Riverhead NY 11901 PhoneNumber: 631-727-7850 x2 Email: ProjectTitle: Botrytis Efficacy Protocol #: 14-001 PR# Research Target Crop/Plant Product EPA Reg. # Production Site Status 32345 Botrytis elliptica Botrytis elliptica Lily Lilium sp. BAS703 06F/BAS703 01F Fluxapyrosad + pyraclostrobi - BASF Field In-Ground C 32351 Botrytis elliptica Botrytis elliptica Lily Lilium sp. Decree Fenhexamid 66330-35-6769 SePro Field In-Ground C 32346 Botrytis elliptica Botrytis elliptica Lily Lilium sp. F9110 F9110 - FMC Field In-Ground C 32350 Botrytis elliptica Botrytis elliptica Lily Lilium sp. Mural (A18126B) WDG Benzovindiflupyr + Azoxystro - Syngenta Field In-Ground C 31356 Botrytis elliptica Botrytis elliptica Lily Lilium sp. Proud 3 Thyme oil (5.6%) - Biohumanetics Field In-Ground C 32347 Botrytis elliptica Botrytis elliptica Lily Lilium sp. S2200 4SC Mandestrobin - Valent Field In-Ground C 32348 Botrytis elliptica Botrytis elliptica Lily Lilium sp. SP2770 10WP SP2770 - SePro Field In-Ground C 32349 Botrytis elliptica Botrytis elliptica Lily Lilium sp. SP2773 SP2773 - SePro Field In-Ground C 31357 Botrytis elliptica Botrytis elliptica Lily Lilium sp. Torque 3.6SC Tebuconazole 1001-87 Cleary Field In-Ground C

Ornamental Horticulture Program Research Report Form p 1 Researcher: Nora Catlin Date: 4/6/2015 Project Title: Efficacy of Management Tools for Botrytis Protocol #: 14-001 PRnumbers: Narrative Summary (Results/Discussion) Nine products were evaluated for efficacy against Botrytis eliptica on lily Vermeer. Products tested included: fluxapryoxad+pyraclostrobin (BAS703 01F), F9110, thyme oil (Proud 3), mandestrobin (S2200), SP2770, SP2773, tebuconazole (Tourque), benzoviniflupyr+azoxystrobin (Mural, A18126B), and fenhexamid (Decree). An untreatedinoculated control and an untreated uninoculated control were also included. No disease symptoms were observed at the first evaluation on 16 July, but symptoms were observed on some treatments by the second evaluation on 22 July (Table 1, Figure 1). By the third evaluation on 8 August, some replicates of all treatments were exhibiting some symptoms. The untreated uninoculated plants and the plants treated with fluxapryoxad+pyraclostrobin (BAS70301F), and benzovindiflupyr+azoxystrobin (Mural) consistently had the lowest disease severity ratings. Mandestrobin (S2200) had significantly lower disease severity ratings than the untreated inoculated plants on the 8 Aug and the 15 Aug evaluations. Some were intermediate in their efficacy for the 22 July, 30 July, and 8 Aug evaluations, being neither significantly different from the untreated inoculated nor the untreated uninoculated. On the 22 July evaluation, those treatments were: Fenhexamid (Decree), Mandestrobin (S2200), SP2770, SP2773, Tebuconazole (Tourque), and Thyme oil (Proud 3). On the 30 July evaluation, those treatments were: Fenhexamid (Decree), Mandestrobin (S2200), and SP2773. On 8 Aug evaluation, those treatments were: Fenhexamid (Decree), SP2770, and SP2773. It was surprising that fenhexamid (Decree) was only intermediate at these evaluations, as this product is one of the best options for managing Botrytis cinerea in greenhouse ornamentals. At the final evaluation, only the fluxapryoxad+pyraclostrobin (BAS70301F), mandestrobin (S2200), and benzovindiflupyr+azoxystrobin (Mural) treatments resulted in reduced severity ratings and number of affected leaves compared to the untreated inoculated control. There were no significant differences in initial plant height and width or final plant height (Table 2.) The conditions for disease development were ideal and a worst case scenario. The lily cultivar Vermeer is known to be very susceptible to Botrytis blight, and plants were kept in a low tunnel fitted with shade cloth and were irrigated with overhead sprinklers. The untreated uninoculated plants remained mostly free from disease, despite the fact that they were kept not far away, approximately 15 20 feet, from the inoculum plants and the other plants in the trial. The untreated uninoculated plants were kept in an un shaded section of the low tunnel (with better drying conditions and higher light), demonstrating the importance or environmental management of Botrytis. It is possible that some products with intermediate efficacy the might have had better performance under conditions that were less ideal for disease development. These products, particularly those still moderately effective at the 30 July and the 8 Aug evaluations, are worthwhile to use in a rotation, or if the disease pressure is low. However, the products with the best efficacy are clearly fluxapryoxad+pyraclostrobin (BAS70301F) and benzovindiflupyr+azoxystrobin (Mural), with Mandestrobin (S2200) being a close second.

Ornamental Horticulture Program Research Report Form p 2 Researcher: Nora Catlin Date: 4/6/2015 Project Title: Efficacy of Management Tools for Botrytis Protocol #: 14-001 PRnumbers: Results Tables Table 1. Average disease severity rating for lily Vermeer plants treated with various management products and untreated plants 16 July 22 July 30 July 8 Aug 15 Aug Treatment Disease severity rating a Disease severity rating Disease severity rating Disease severity rating Disease severity rating Fluxapryoxad+pyraclostrobin (BAS703 01F), 8 oz/100 gal 0 0.0 c 0.1 c 0.8 c 0.6 b F9110, 24 oz/100 gal 0 1.1 ab 2.9 a 3.8 ab 6.9 a Thyme oil, 5.6% (Proud 3), 4 qt/100 gal 0 1.0 abc 2.1 ab 3.6 ab 6.8 a Mandestrobin (S2200), 7.5 oz/100 gal 0 0.3 abc 1.3 abc 1.3 bc 2.3 b SP2770 10WP, 2.66 lb/100 gal 0 1.0 abc 2.1 ab 3.0 abc 6.5 a SP2773, 3.31 lb/100 gal 0 1.0 abc 1.5 abc 3.0 abc 7.5 a Tebuconazole (Tourque), 8 oz/100 gal 0 0.9 abc 2.4 a 3.9 a 6.9 a Benzoviniflupyr+azoxystrobin (Mural, A18126B), 7 oz/100 gal 0 0.0 c 0.4 bc 1.0 c 2.1 b Untreated uninoculated 0 0.1 bc 0.4 bc 0.9 c 1.3 b Untreated inoculated 0 1.3 a 2.6 a 5.0 a 8.3 a Fenhexamid (Decree), 1.5 lb/100 gal 0 1.0 abc 1.1 abc 3.1 abc 6.8 a a Disease severity was rated on a scale from 0 to 10 (where 0=no symptoms and 10=100% of leaves affected by symptoms). Means within a column with similar letters are not significantly different according to Tukey s HSD (p=0.05)

Ornamental Horticulture Program Research Report Form p 3 Researcher: Nora Catlin Date: 4/6/2015 Project Title: Efficacy of Management Tools for Botrytis Protocol #: 14-001 PRnumbers: Figure 1. Average disease severity rating for lily Vermeer plants treated with various management products and untreated plants.

Ornamental Horticulture Program Research Report Form p 4 Researcher: Nora Catlin Date: 4/6/2015 Project Title: Efficacy of Management Tools for Botrytis Protocol #: 14-001 PRnumbers: Table 2. Average initial height and width measurements and final height measurements of lily Vermeer plants treated with various management products and untreated control plants. Treatment Initial Height (in) Initial Weight (in) Final Height (in) Fluxapryoxad+pyraclostrobin (BAS703 01F), 8 oz/100 gal 25.3 a 6.6 a 28.1 a F9110, 24 oz/100 gal 24.4 a 6.7 a 26.8 a Thyme oil, 5.6% (Proud 3), 4 qt/100 gal 24.2 a 6.2 a 27.3 a Mandestrobin (S2200), 7.5 oz/100 gal 24.8 a 6.9 a 28.0 a SP2770 10WP, 2.66 lb/100 gal 24.6 a 6.7 a 26.8 a SP2773, 3.31 lb/100 gal 24.3 a 6.6 a 26.7 a Tebuconazole (Tourque), 8 oz/100 gal 25.0 a 7.0 a 27.9 a Benzoviniflupyr+azoxystrobin (Mural, A18126B), 7 oz/100 gal 24.6 a 6.9 a 27.7 a Untreated uninoculated 25.0 a 6.5 a 27.8 a Untreated inoculated 24.6 a 7.1 a 27.1 a Fenhexamid (Decree), 1.5 lb/100 gal 24.2 a 6.9 a 26.1 a Means within a column with similar letters are not significantly different according to Tukey s HSD (p=0.05)

Ornamental Horticulture Program Research Report Form p 5 Researcher: Nora Catlin Date: 4/6/2015 Project Title: Efficacy of Management Tools for Botrytis Protocol #: 14-001 PRnumbers: Materials & Methods/Recordkeeping Name(s) of Personnel Conducting Research: Nora Catlin (PI), Adam Hubert (Program Assistant) Location of Trial (city/state): Riverhead, NY Use Site (greenhouse/shadehouse/field container/etc): shade house Crop History Crop Cultivar/Variety: Date of Seeding: Date of Emergence: Date of Transplanting: Potting Mix: Pot size & spacing: Row spacing: Lily hybrid, Vermeer n/a n/a 9 June ProMix BX 4.5 inch container, pot to pot spacing n/a Product(s) applied prior to start of experiment: Product Rate Application Type Date of Application Crop Growth Stage Application Volume

Ornamental Horticulture Program Research Report Form p 6 Researcher: Nora Catlin Date: 4/6/2015 Project Title: Efficacy of Management Tools for Botrytis Protocol #: 14-001 PRnumbers: Experiment Information Experimental Design: Number of Reps: CRD 8 single plant replicates Materials & Methods: Lily Vermeer planted on 9 June into 4.5 inch pot and kept in greenhouse until after the first treatment. After the first treatment, plants were moved into a hoop house fitted with shade cloth and overhead irrigation in order to provide ideal disease conditions. Containers of symptomatic plants were placed among the plants to serve as inoculum and the untreated uninoculated control plants were set on the opposite side of the house, in an un shaded section a distance away from the inoculum. When necessary, plants were supplemented with hand watering. Treatments were applied every 2 weeks for 6 weeks, starting on 9 July 2014 (30 d after planting), with the exception of F9110, thyme oil, and fenhexamid, which were applied weekly. Plants were evaluated for disease symptoms weekly, starting one week after treatment. Where applicable, data were subject to ANOVA and means were separated using Tukey s HSD (p=0.05). Application Equipment: 30 psi. applied to drip using a CO2 powered sprayer fitted with a TeeJet 8003 nozzle at Product(s) applied during experiment (including treatments, fertilizers, etc): Application Type Date of Application Application Volume Product Rate(s) Crop Growth Stage Fluxapryoxad+pyraclostrobin (BAS703 01F) 8 oz/100 gal Spray 9 July 29 d after planting, bud 240 ml F9110 24 oz/100 gal Spray 9 July 29 d after planting, bud 240 ml Thyme oil, 5.6% (Proud 3) 4 qt/100 gal Spray 9 July 29 d after planting, bud 240 ml Mandestrobin (S2200) 7.5 oz/100 gal Spray 9 July 29 d after planting, bud 250 ml SP2770 10WP 2.66 lb/100 gal Spray 9 July 29 d after planting, bud 250 ml SP2773 3.31 lb/100 gal Spray 9 July 29 d after planting, bud 250 ml Tebuconazole (Tourque) 8 oz/100 gal Spray 9 July 29 d after planting, bud 230 ml Benzoviniflupyr+azoxystrobin (Mural, A18126B) 7 oz/100 gal Spray 9 July 29 d after planting, bud 250 ml Fenhexamid (Decree) 1.5 lb/100 gal Spray 9 July 29 d after planting, bud 230 ml F9110 24 oz/100 gal Spray 17 July 29 d after planting, bud 250 ml Thyme oil, 5.6% (Proud 3) 4 qt/100 gal Spray 17 July 29 d after planting, bud 250 ml SP2770 10WP 2.66 lb/100 gal Spray 17 July 29 d after planting, bud 260 ml Fenhexamid (Decree) 1.5 lb/100 gal Spray 17 July 29 d after planting, bud 250 ml

Ornamental Horticulture Program Research Report Form p 7 Researcher: Nora Catlin Date: 4/6/2015 Project Title: Efficacy of Management Tools for Botrytis Protocol #: 14-001 PRnumbers: Fluxapryoxad+pyraclostrobin (BAS703 01F) 8 oz/100 gal Spray 23 July F9110 24 oz/100 gal Spray 23 July Thyme oil, 5.6% (Proud 3) 4 qt/100 gal Spray 23 July Mandestrobin (S2200) 7.5 oz/100 gal Spray 23 July SP2770 10WP 2.66 lb/100 gal Spray 23 July SP2773 3.31 lb/100 gal Spray 23 July Tebuconazole (Tourque) 8 oz/100 gal Spray 23 July Benzoviniflupyr+azoxystrobin (Mural, A18126B) 7 oz/100 gal Spray 23 July Fenhexamid (Decree) 1.5 lb/100 gal Spray 23 July F9110 24 oz/100 gal Spray 31 July Thyme oil, 5.6% (Proud 3) 4 qt/100 gal Spray 31 July SP2770 10WP 2.66 lb/100 gal Spray 31 July Fenhexamid (Decree) 1.5 lb/100 gal Spray 31 July Fluxapryoxad+pyraclostrobin (BAS703 01F) 8 oz/100 gal Spray 7 Aug F9110 24 oz/100 gal Spray 7 Aug Thyme oil, 5.6% (Proud 3) 4 qt/100 gal Spray 7 Aug Mandestrobin (S2200) 7.5 oz/100 gal Spray 7 Aug SP2770 10WP 2.66 lb/100 gal Spray 7 Aug SP2773 3.31 lb/100 gal Spray 7 Aug Tebuconazole (Tourque) 8 oz/100 gal Spray 7 Aug Benzoviniflupyr+azoxystrobin (Mural, A18126B) 7 oz/100 gal Spray 7 Aug Fenhexamid (Decree) 1.5 lb/100 gal Spray 7 Aug 45 d after planting, bud or open bloom 45 d after planting, bud or open bloom 45 d after planting, bud or open bloom 45 d after planting, bud or open bloom 45 d after planting, bud or open bloom 45 d after planting, bud or open bloom 45 d after planting, bud or open bloom 45 d after planting, bud or open bloom 45 d after planting, bud or open bloom 53 d after planting, open bloom 53 d after planting, open bloom 53 d after planting, open bloom 53 d after planting, open bloom 60 d after planting, open bloom 60 d after planting, open bloom 60 d after planting, open bloom 60 d after planting, open bloom 60 d after planting, open bloom 60 d after planting, open bloom 60 d after planting, open bloom 60 d after planting, open bloom 60 d after planting, open bloom 250 ml 255 ml 250 ml 250 ml 255 ml 255 ml 250 ml 250 ml 250 ml 250 ml 260 ml 260 ml 260 ml 240 ml 260 ml 260 ml 260 ml 260 ml 260 ml 250 ml 250 ml 250 ml

Ornamental Horticulture Program Research Report Form p 8 Researcher: Nora Catlin Date: 4/6/2015 Project Title: Efficacy of Management Tools for Botrytis Protocol #: 14-001 PRnumbers: Photos See photos in folder 2014 Botrytis Management NCatlin_Photos ; includes photos taken of all treatments on 7 August and 15 August. Data Collected Plants were evaluated and rated for disease severity approximately weekly, starting approximately 1 week after the first treatment. Evaluations were conducted on 16 July, 22 July, 30 July, 8 Aug, and 15 Aug. Disease severity was rated on a scale from 0 to 10 (where 0=no symptoms and 10=100% of leaves affected by symptoms). The number of affected leaves were also recorded on 30 July, 8 Aug, and 15 Aug, however this data did not practically vary from the disease severity ratings and is not presented herein. Initial plant height and width was recoded approximately 1 week after the first treatment on 16 July, and final plant height was recorded on 19 Aug. Final plant width was not recorded since disease affected plants had either desiccated or dropped leaves and width measurements would not have provided meaningful data. Raw Data See file: 2014 Botrytis Management NCatlin

Ornamental Horticulture Program Research Report Form p 9 Researcher: Nora Catlin Date: 4/6/2015 Project Title: Efficacy of Management Tools for Botrytis Protocol #: 14-001 PRnumbers: Environmental conditions during the experiment: From planting until the day after the first treatment, plants were maintained in a greenhouse with an average daily temperature of 77F. After the first treatment plants were moved to a shade tunnel, and were irrigated with an overhead sprinkler system for approximately 10 minutes twice per day. Occasionally, plants were supplemented with hand watering, which was necessary of very hot and sunny days particularly for the untreated uninoculated plants which were in a sunny area. The average high temperature during the trial was 81F, the average low temperature was 65F, and the average daily temperature was 73F. There were 5 days of precipitation, accumulating a total of 3.7 inches of rain. There were no unusual weather events during the trial.

N. Catlin - F9110-Aug 7

N. Catlin - Decree_Aug 7

N. Catlin - BAS70301F-Aug 7

N. Catlin - Torque_Aug 7

N. Catlin - SP2773_Aug 7

N. Catlin - SP2770_Aug 7

N. Catlin - S2200_Aug 7

N. Catlin - Proud3_Aug 7

N. Catlin - Mural_Aug 7

N. Catlin - Untrt-Uninoc_Aug 7

N. Catlin - Untrt-Inoc_Aug 7

N. Catlin - S2200_Aug 15

N. Catlin - Proud3_Aug 15

N. Catlin - Mural_Aug 15

N. Catlin - F9110_Aug 15

N. Catlin - Decree_Aug 15

N. Catlin - BAS70301F_Aug 15

N. Catlin - Untrt-Uninoc_Aug 15

N. Catlin - Untrt-Inoc_Aug 15

N. Catlin - Torque_Aug 15

N. Catlin - SP2773-Aug 15

N. Catlin - SP2770_Aug 15