Hishtil R.M Ltd HTS (High Throughput System) for root traits selection 1
The challenge Improve plant performance by direct root breeding. (The hidden-part of the plant) 2
Low soil temperature causes leaf dehydration and growth delay 3
Improvement of root performance, is hindered by the absence of efficient selection system. Tolerant wild Tomato Sensitive Commercial tomato4
The solution HTS by Hishtil R.M Practical selection method, without plant destruction High correlation with field results. 5
HTS advantages Controlled conditions. Large scale screening (10,000 s s of plants) in few weeks. Work all year round, no weather limitations. 6
HTS in seedling stage 7
HTS applications Identify cold tolerant lines. Prove potential heritability. Selection procedure for grafted plants. High correlation between HTS and field. 8
Tomato screening Source Populations Tolerant % Known hyb. 270 7 2.6 Selected lines 40 11 27.5 9
Tomato reactions to 5 o c T (Tolerant) Tolerant line MT (Medium Tolerant) Beaufort S (Sensitive) Shirley 10
Trait heritability (field) M 82 Sensitive T Tolerant F1 X T Tolerant 11
Cold Soil Tolerant Processing Tomato (2009 Trial) 12
Acre field test, Israel (Spring 2009) The seedlings where produced and grafted in Hishtil nursery Planting 9/2/2009 = 50 days prior to standard planting season Field Trial: Included 37 different rootstocks The rootstocks were grafted : - Non grafted - On themselves -With as scion - With Heinz 9780 as scion Notes: Lines HRM17 and HRM19 are cold tolerant genetic material under development by Hishtil RM. 13
Tomato HTS at 5 o c Example: 80 X HRM 19 M S T T Beaufort Shirley Tolerant line 80 X HRM19 14
T (80 X HRM19) S (23/4/2009) (19/3/2009) 15
T (80 X HRM19) S (21/5/2009) Fruits weight per plant (g) Rootstock Very small Small Medium Large Red Total 80xHRM19 156.7 980.0 2,072.0 1,500.0 213.3 4,922.0 Beaufort 150.0 73.3 896.7 383.3 1,360.0 778.0 540.0 906.7 86.7 0.0 2,883.3 2,068.0 16
T (80 X HRM19) S (10/6/2009) Fruits weight per plant (g) Rootstock Small Medium Large Red Total 80xHRM19 154.0 1,511.3 2,746.7 5,393.3 9,805.3 Beaufort 16.0 1,382.7 1,431.3 2,443.3 5,273.3 46.7 928.7 2,436.7 1,791.3 5,203.3 17
T (80 X HRM19) S (21/5/2009) (10/6/2009) 18
Fruit weight per plant 10.6.09 (80 X HRM19) T 6 6 5 21.5.09 5 80XHRM19 4 3 2 80xHRM19 4 1 0 Beaufort 3 Beaufort Red Large Medium Small Very small 2 1 0 Red Large Medium Small 19
Heinz 9780 (80 X HRM19) T Heinz 9780 Heinz 9780 S (21/5/2009) Fruits weight per plant (g) Rootstock Very small Small Medium Large Total 80xHRM19 310.0 1,233.3 1,200.0 1,693.3 4,436.7 Beaufort 150.0 540.0 960.0 210.0 1,860.0 Heinz9780 83.3 633.3 483.3 323.3 1,523.3 20
Heinz 9780 T (80 X HRM19) Heinz 9780 Heinz 9780 S (18/6/2009) Fruits weight per plant (g) Rootstock Small Medium Large Red Total 80XHRM19 24.7 914.7 5,763.3 7,548.0 14,250.7 Beaufort Heinz9780 43.3 31.0 606.7 460.0 2,043.3 1,680.0 3,016.7 2,600.0 5,710.0 4,771.0 21
Heinz 9780 T (80 X HRM19) (21/5/2009) Heinz 9780 Heinz 9780 S (18/6/2009) 22
Fruit weight per plant 18.6.09 Heinz9780 (80 X HRM19) T 8 80XHRM19 7 6 5 4 3 2 Beaufort Heinz9780 1 0 Red Large Medium Small 23
Tomato HTS at 5 o c : Example HRM19 X HRM 17 BeaufortM Shirley S Tolerant line T HRM19 XHRM17 T 24
T (HRM19 X HRM17) S (23/4/2009) (19/3/2009) 25
(HRM19 X HRM17) S (21/5/2009) 26
T (HRM19 X HRM17) S (10/6/2009) Fruits weight per plant (g) Rootstock Small Medium Large Red Total HRM19XHRM17 73.3 1,716.7 1,306.7 4,386.7 7,483.3 Beaufort 16.0 46.7 1,382.7 928.7 1,431.3 2,436.7 2,443.3 1,791.3 5,273.3 5,203.3 27
T (HRM19 X HRM17) S (21/5/2009) (10/6/2009) 28
Fruit weight per plant 10.6.09 (HRM19 X HRM17) T 6 6 5 21.5.09 5 HRM19XHRM17 4 3 4 2 1 Beaufort HRM19XHRM17 3 0 Red Large Medium Small Very small 2 Beaufort 1 0 Red Large Medium Small 29
Heinz 9780 T (HRM19 X HRM17) Heinz 9780 Heinz 9780 S (23/4/2009) (19/3/2009) 30
Heinz 9780 T Heinz 9780 S (HRM19 X HRM17) Heinz 9780 (18/6/2009) Fruits weight per plant (g) Rootstock Small Medium Large Red Total HRM19XHRM17 91.3 486.7 2,746.7 4,558.0 7,882.7 Beaufort Heinz9780 43.3 31.0 606.7 460.0 2,043.3 1,680.0 3,016.7 2,600.0 5,710.0 4,771.0 31
Fruit weight per plant 18.6.09 6 5 HRM19XHRM17 4 3 Beaufort Heinz9780 2 1 0 Red Large Medium Small 32
Different rootstocks fruit weight as scion 33
Fruit weight per plant 10.6.09 6 5 80XHRM19 HRM19XHRM17 4 80XHRM17 3 ShXHRM19 Beaufort 2 1 Heinz9780 0 Red Large Medium Small 34
Different rootstocks fruit weight Heinz9780 as scion 35
Fruit weight per plant 18.6.09 8 80XHRM19 7 6 5 80XHRM17 HRM19XHRM17 4 3 2 Beaufort Heinz9780 1 0 Red Large Medium Small 36
Tolerant versus Sensitive Plants Selection at 5 o C Control 158 Commercial Rootstock T Tolerant T Tolerant 37
Tolerant versus Sensitive Plants (In the Field) Tolerant Hybrid T Sensitive Line S Tolerant Hybrid T 38
Different Rootstocks with same commercial scion Commercial line Commercial line Commercial line Sensitive Line Commercial line Tolerant Hybrid Control Sensitive Tolerant 39
כנות scion שונות עם רוכב ז commercial הה (זן with מסחרי) שתיל Rootstocks ת ספטמבר Different (September planting) Commercial line Sensitive Line Commercial line Tolerant Hybrid 40
Different Rootstocks with commercial scion (September planting) Commercial line Commercial line Commercial line Sensitive Line Commercial line Tolerant Hybrid 80 C m 130 C m 41
Summary Tolerant genetic material was identified and is being developed. The selected lines were used as rootstock to demonstrate the contribution of the root trait to the ability of the plant to grow under low soil temperature The rootstock s contribution is dominating The trait is heritable High Throughput Selection results correlate with field outcomes The system is applicable to multiple crops 42
Hishtil R.M Ltd Thank you 43