RapidVap Dry Evaporation Systems

Transcription

1 RapidVap Dry Evaporation Systems

2 2 RapidVap Dry Evaporation Systems An Overview Page An Overview 2 RapidVap Vertex Dry Evaporation Systems Features and Benefits 4 Specifications 6 Evaporation Rates 7 RapidVap Vacuum Evaporation Systems Features and Benefits 9 Control Panel 10 Evaporation Rates 10 Specifications 11 Accessories 12 RapidVap N 2 and N 2 /48 Evaporation Systems Control Panel 16 RapidVap N 2 Evaporation Zone and Cool-Zone 17 RapidVap N 2 Evaporation Rates 18 RapidVap N 2 Specifications 19 RapidVap N 2 /48 Evaporation Rates 20 RapidVap N 2 /48 Specifications 21 Accessories 22 RapidVap Recovery Data 24 RapidVap Evaporation Systems are multiple-sample evaporators that use a combination of gentle forces to quickly reduce samples to dryness or an end point volume. RapidVap Systems are built to resist attack from strong chemicals. The chamber and sample block are PTFE-coated and all mechanical components such as bearings are isolated from chemical fumes and vapors. All three RapidVap Systems utilize dry heat, feature robust programmability and have greater throughput when compared to rotary evaporators. Read below for details on each system. RapidVap Vertex Dry Evaporation Systems Using dry heat and nitrogen blow down, the RapidVap Vertex Dry Evaporator speeds evaporation of up to 50 samples at the same time. The Vertex accommodates 1.5 milliliter microcentrifuge tubes up to 60ml ASE* vials. A convenient touchpad allows easy programming of time and temperature. Up to ten different programs may be stored in the microprocessor for protocol consistency. Unlike water bath heaters, the dry block heater requires less maintenance, no distilled water or additives, and adds no potential source of contamination. The RapidVap Vertex Evaporator is ideal for preparation of samples in a variety of applications including drug discovery, clinical analysis, environmental testing, agrochemistry, and forensic evidence processing. * ASE is a registered trademark of Dionex, Sunnyvale, California. Scan here to watch a video and learn how the RapidVap Vertex quickly evaporates samples using dry heat and nitrogen blow down

3 RapidVap Dry Evaporation Systems 3 RapidVap Vacuum Dry Evaporation Systems The RapidVap Vacuum System is ideal for preparation of samples in a variety of applications including drug discovery, agrichemistry, mycology testing and environmental analysis. The system uses vacuum along with heat and vortex motion to help speed evaporation and provides an alternative to traditional centrifugal evaporation methods. A microprocessor provides precise control of the vacuum level so that bumping can be minimized, preventing loss of sample. A port on the back of the RapidVap Vacuum System allows attachment to an accessory diaphragm or rotary vane vacuum pump. The RapidVap Vacuum System accommodates seven different, interchangeable accessory sample blocks holding sample volumes from just a few milliliters to as large as 450 milliliters. The blocks hold more samples than ever before, offering greater throughput. As many as 110 each 12 or 13 mm tubes may be loaded at once. Additional blocks accommodate various tube types including conical centrifuge tubes and scintillation vials. Custom blocks to fit special glassware configurations such as VOA and ASE* vials are also available. The RapidVap Vacuum Evaporation System uses vaccum, heat and vortex motion to speed evaporation. RapidVap N 2 and N 2 /4B Evaporation Systems The RapidVap N 2 and N 2 /48 Systems offer a more efficient, automatic alternative to Kuderna-Danish and rotary evaporators. In these systems, a stream of nitrogen or dry gas is directed downward onto the surface of the sample. Nitrogen blow down reduces the partial pressure directly over the liquid to speed evaporation and help remove the solvent as it evaporates. Nitrogen may be programmed for two, four, six or all eight tube locations. On the RapidVap N 2 /48, eight clusters of six ports each allow for up to 48 small samples. Various blocks hold different sample sizes, from 4.5 to 26 milliliters. The block that comes with the RapidVap N 2 holds eight large samples, up to 450 milliliters each, making it efficient for environmental and residue testing. An accessory block that holds eight 170 milliliter tubes is also available. In many of these applications, the samples should not go to dryness. The unique Cool-Zone block and tube design significantly reduces the evaporation rate after end point is achieved. The Cool-Zone insulates the samples and allows the researcher adequate time before the samples reach dryness to remove them or to perform solvent exchange procedures. Alarms that signal when timed end point has occurred and when the RapidVap senses that the process is nearing The gentle agitation of the sample tube causes the liquid to form a vortex-like configuration, which increases the sample s surface area and ability to evaporate. completion allow for unattended operation. In the RapidVap N 2 /48 Evaporation System, nitrogen blow down, heat and vortex motion combine to quickly reduce multiple samples.

4 4 RapidVap Vertex Dry Evaporation Systems Nitrogen pressure regulator Angled samples Five rows of 10 nozzles deliver nitrogen to up to 50 samples LCD display with touchscreen programming Nine interchangeable blocks are available (sold separately). See page 7. Custom blocks are also available. Valves control five rows of 10 nozzles that deliver nitrogen to up to 50 samples The NitroVap 2LV Nitrogen Gas Generator by Parker Hannifin is available (sold separately). See page Made in U.S.A.

5 RapidVap Vertex Dry Evaporation Systems 5 Features & Benefits Evaporate up to 50 samples at once for maximum throughput. Nine blocks available. Up to 50 samples may be processed at once. Seven blocks are offered that hold 50 sample tubes, with sample sizes from 1 to 30 milliliters. Two additional blocks hold 18 ASE* sample tubes, with sample sizes from 40 to 60 milliliters. Aluminum blocks are interchangeable and sold separately. See page 7. Dry heat no water bath is used. Dry heat has several advantages over water bath heat: less maintenance (no residue built up), no rust, no condensation and no potential source of cross contamination. Microprocessorcontrolled heater supplies heat to the block and is programmable from 30 C up to 100 C in 1 degree increments. Fast evaporation with nitrogen blow down and heat. Nitrogen blow down reduces the partial pressure directly over the liquid to speed evaporation and help remove the solvent as it evaporates. Heat helps speed the process. See the evaporation rates on page 7. See recovery rates on page 24. Angled samples increase surface area for faster evaporation, optimized recovery. The evaporator holds the samples at an angle to maximize surface area where evaporation occurs. Glass lid. Provides visibility of samples and is resistant to chemicals. The lid may be lifted during a run to check on, add or remove sample tubes. Convenient, easy-to-set touchscreen programming and display. Touching the screen allows parameters of program number, preheat temperature from 30 C to 100 C, and time from 1 to 999 minutes to be easily set. Up to 10 different user-set programs, each with different parameter set points, may be stored in memory. During the run, actual temperature and time elapsed are displayed. At any time during the run, the user may stop the program by pressing PAUSE, which shuts off the nitrogen flow and pauses the timer, but keeps the heater active. The user may lift the lid and add or remove samples. Once the lid is closed, the user may resume the program by pressing RESTART. Temperature sensor monitors block or sample temperature. Sensor fits into a port in the upper left corner of any block to monitor block temperature or may be placed in a sample to monitor sample temperature. Temperature is displayed on the LCD screen. Five nitrogen control valves with easy on/off switches. Turn nitrogen flow on or off to the nozzles above the sample vials. Each valve controls a row of 10 nozzles allowing nitrogen to be conserved during partial runs. Easy-to-access, built-in nitrogen pressure regulator. Located in easy reach on the front of the evaporator, the regulator controls the pressure of the nitrogen that is delivered to the samples. Reliability guarantee. Full one year warranty is provided against defects in materials and workmanship. Compact benchtop design. Small footprint fits in tight spaces. When placed on a lab bench, the evaporator s rear port connects to the exhaust hose for routing into a fume hood. ETL listed. Models and carry the ETL Testing Laboratories seal in the U.S. and carry the ETL-C seal in Canada, signifying it meets or exceeds all requirements of UL Standard A-1 and CAN/CSA C22.2 No CE Mark. Models , and conform to the CE (European Community) requirements for electrical safety and electromagnetic compatibility. * ASE is a registered trademark of Dionex, Sunnyvale, California. Exclusive feature

6 6 RapidVap Vertex Dry Evaporation Systems Specifications Powder-coated steel exterior. Glass lid. 900 watt dry block heating system. 50 nitrogen-dispensing nozzles in five horizontal rows of nitrogen control valves with on/off switches. Front-mounted pressure regulator with analog display of pressure from 0 to 45 psi in 2 psi increments. LCD with touchscreen programming and display of program number; set point temperature and actual system and sample/block temperatures in C or F; set point time and time remaining. Microprocessorcontrolled programming includes program number from 1 to 10, temperature of the system from 30 to 100 C (86 to 212 F), and time from 1 to 999 minutes or ON. Memory stores 1 to 10 programs. Temperature sensor probe for monitoring of block or sample temperature. Built-in exhaust fan with blower. On/off switch. 6 feet (183 cm) of 2" (5 cm) ID polyethylene exhaust hose, with clamp. 6 feet (183 cm) of flexible polyethylene tubing for nitrogen supply, with push-to-connect fitting. 1 year warranty on materials and workmanship. ETL and ETL-C listing on models and CE mark on models , and Overall dimensions with closed lid: 20.4" wide x 13.0" deep x 12.5 high (51.8 x 33.1 x 31.6 cm) Overall dimensions with open lid: 20.4" wide x 13.0" deep x 22.7" high (51.8 x 33.1 x 57.7 cm) Actual weight 35.0 lbs. (15.9 kg) Shipping weight 45.0 lbs. (20.4 kg) Made in U.S.A. Accessories required (not included) Aluminum Block. See page 7. Sample tubes. Contact your laboratory supply distributor. Nitrogen source with flow rate of 6.5 CFM/185 LPM minimum. Nitrogen pressure not to exceed 50 psi. Gas flow with 10 active nozzles is approximately psi and psi. Gas flow with 50 active nozzles is approximately psi and psi. A nitrogen generator is recommended. See page 23 for ordering information on the Nitro 2LV Nitrogen Generator or contact your nitrogen gas supplier. Ordering Information Catalog Number Electrical Requirements Power Cord & Plug Type volts, 60 Hz, 8 amps NEMA 5-15P * 230 volts, 50/60 Hz, 4 amps Schuko * 230 volts, 50/60 Hz, 4 amps British (UK) * 230 volts, 50/60 Hz, 4 amps China/Australia volts, 50/60 Hz, 4 amps North America, 230 Volt 115 volts,15 amps For * International electrical configuration Schuko For British (UK) For China/Australia For North America, 230 volts For

7 RapidVap Vertex Dry Evaporation Systems 7 Evaporation Rates Solvent Tube Size Number Sample Temperature N 2 Pressure Avg. Time (m m) of Samples Size (ml) ( C) (psi) to Dry (min.) Acetonitrile 12 x < x <15 12 x <11 12 x <8 Methanol 12 x <12 12 x <10 12 x <7 12 x <6 12 x <6 20 x <42 Water 12 x < x <80 12 x <40 12 x <25 20 X <60 20 X <64 Toluene 12 x <24 12 x <18 12 x <13 12 x <9 Methylene 12 x <8 Chloride 12 x <7 20 x <22 Hexane 20 x <11 Ethyl Acetate 20 x <22 A B C D E F G Aluminum Blocks All RapidVap Vertex Dry Evaporators require a Block (not included). Blocks of solid aluminum include stainless steel handles to lift the block in and out of the evaporator. A 0.21" (0.5 cm) diameter port in the upper left hand corner of the block is provided for insertion of the temperature probe to monitor upper block temperature. Select a block that closely matches the outside diameter of your sample container. Custom blocks to fit special glassware are available upon request. Glassware is required (not included). Catalog Number Block Tube Capacity Sample Tube Size/Description Sample Volume Shipping Weight (A) x 75 mm tube 5 ml 26.0 lbs. (11.8 kg) (B) x 100 mm tube 8 ml 24.0 lbs. (10.9 kg) (C) x 125 mm tube 16 ml 21.0 lbs. (9.5 kg) (D) x 150 mm tube 30 ml 14.9 lbs. (6.8 kg) (E) x 75 mm tube 3 ml 26.0 lbs. (11.8 kg) (F) x 95 mm ASE vial 40 ml 22.0 lbs. (10.0 kg) (G) x 140 mm ASE vial 60 ml 19.0 lbs. (8.6 kg) * ml microcentrifuge tube 1 ml 33.0 lbs. (15.0 kg) * x 100 mm tube 12 ml 19.0 lbs. (8.6 kg) * x 120 mm 15 ml conical tube 12 ml 33.0 lbs. (15.0 kg) * Not shown

8 8 RapidVap Vacuum Dry Evaporation Systems Phenol-free gasket Glass lid with polycarbonate shield Dual lid clamps Easy-to-read LCD display Microprocessorcontrolled 1000-watt dry block heating system PTFE-coated aluminum sample block (sold separately) Microprocessorcontrolled motor PTFE-coated aluminum chamber Made in U.S.A.

9 RapidVap Vacuum Dry Evaporation Systems 9 Features and Benefits Faster evaporation rates, greater throughput. Vortex action, heat and vacuum combine to accelerate evaporation. Up to 110 samples may be processed at once. See page 10 for evaporation rates. See page 25 for recovery rates. Microprocessor-controlled vacuum level speeds evaporation. Microprocessor controls vacuum at a preset level. Rear-mounted 1/2" OD port attaches to a vacuum pump (sold separately). Automatic vacuum release. Vacuum is automatically released in the event of power failure. Microprocessor-controlled 1000-watt dry block heating system. Speeds evaporation rate by supplying a controlled amount of heat from ambient up to a maximum of 100 C to the sample block. The heater and block move in tandem for more efficient heat transfer. Unlike water baths, the dry heating system adds no potential source of contamination and requires no maintenance. Microprocessor-controlled vortex motion increases surface area for faster evaporation. Vortex action continually mixes the sample, helps contain analytes in the solvent, minimizes bumping and maximizes sample recovery. It also performs a time-saving function in resuspension and solvent exchange procedures. Maintenance-free microprocessor-controlled motor. Reliable belt-driven system provides smooth variable-speed power to drive the vortex motion up to 1000 rpm. Non-sparking, brushless DC motor ensures safety, control and reproducibility of protocols. PTFE-coated aluminum chamber. Conducts heat efficiently and resists corrosion. PTFE-coated aluminum sample blocks are interchangeable and hold more tubes than ever before. Blocks are sold separately. See page 12. Custom blocks for other tube configurations are also available. Durable construction with sleek styling. The cabinet base is powder-coated cast aluminum. The upper housing is solvent-resistant, smooth acrylic/pvc thermoplastic. Glass lid with polycarbonate shield. Provides visibility of samples, is chemical resistant and detaches for cleaning. Polycarbonate shield provides protection from an unlikely glass implosion. Dual lid clamps. Two clamps hold the lid securely in place for a vacuum tight seal. Exclusive feature Phenol-free gasket. Provides complete sealing under vacuum without potential for phenol contamination. Alarm signals completion of run allowing samples to be left unattended while evaporating to desired end point. The RapidVap Vacuum System has two means to signal completion of a run. With the first method, the operator sets the end point time and when set time has expired an audible alarm sounds and the RapidVap automatically turns off all functions. With the second method, the RapidVap monitors system temperature in the block and in the heater. Once the system senses that end point is near, the alarm sounds every 10 seconds and the PREHEAT/END ALARM indicator light flashes. Convenient, easy-to-set programming. Parameters of time, heat, vortex speed and vacuum may be easily set with the touch of a few buttons. Up to 9 different user-set programs, each with different parameter set points, may be stored in memory. In addition, the user may alter parameters while a program is in progress. Easy-to-read LCD display. Prompts user to set program parameters and displays program number and actual and set point vortex speeds, temperatures, time remaining and vacuum levels. See details on page 10. Improved chemical compatibility. The operator may use a broad range of aggressive chemicals. Contact Labconco for specific chemical compatibility. All mechanical components are isolated from the chemical fumes and vapors for longer life. ETL listed. All 115 volt models carry the ETL Testing Laboratories seal in the U.S. and ETL-C seal in Canada, signifying they meet or exceed all minimum requirements of UL Standard and CAN/CSA C22.2 No International electrical configurations available. All 230 volt models conform to the CE (European Community) requirements for electrical safety and electromagnetic compatibility. Reliability guarantee. Full one year warranty is provided against defects in materials and workmanship. Optional Lid Heater improves visibility. A 40-watt heater is securely bonded to the glass lid, which electrically connects to the RapidVap via a receptacle on the back of the housing. Heating the lid helps eliminate condensation caused by evaporating high boiling point solvents.

10 10 RapidVap Vacuum Dry Evaporation Systems Control Panel LCD display. Prompts user to set program parameters and displays program number, actual and set point vortex speed by percentage, actual and set point temperature in C, time remaining in minutes, and actual and set point vacuum level in mbar. Preheat/End Alarm button with indicator light. Pressing the button activates the heater to begin elevating the block to set point temperature. When in preheat mode, the operator may monitor actual heat, adding samples and starting program once set point temperature has been reached. During a run, the indicator light flashes when end point has been detected by the temperature sensors. Pressing the button while the light is flashing switches the end point alarm from audible to muted or back to audible. Program parameter. LCD shows program from 1 to 9, R for run, or S for stop. Vacuum Release button with indicator light. Pressing the button momentarily breaks the vacuum to control bumping. Vacuum returns to its preset level once the button is released. Speed parameter. LCD shows speed from 0 to 100%. Increase/Decrease buttons. Used to increase or decrease the last selected set point parameter. Heat parameter. LCD shows heat from OFF to 99 ( C) or HI (100 C). Run/Stop button. Initiates the start or stop of the selected program. Time parameter. LCD shows time selected from 1 to 999 minutes. During the run, the LCD shows time remaining. If RUN is selected instead of a time period, the RapidVap runs continuously. Vacuum parameter. LCD shows vacuum level from 0 to 999 mbar. Above 999 mbar, the display shows HI and the vacuum pump does not operate. Set point selection buttons. To select a parameter to change, press the set point select button directly below the parameter. Evaporation Rates Solvent Temp. Vacuum Overall Evap. Rate n Methylene Chloride 50 C 200 mbar.56 ml/min./tube n Toluene 80 C 200 mbar.40 ml/min./tube n Acetonitrile 75 C 330 mbar.40 ml/min./tube n Water 80 C 133 mbar.08 ml/min./tube Number of samples: 69 Tube diameter: 16 mm Block used: , 69 tube capacity Ending volume: dryness Vortex speed: 95% Vacuum pump displacement capacity: 88 liters/minute Maximum attainable vacuum: 31 mbar Solvent Temp. Vacuum Overall Evap. Rate n Methylene Chloride 50 C 250 mbar 4.8 ml/min./tube n Toluene 80 C 133 mbar 3.3 ml/min./tube n Acetonitrile 75 C 167 mbar 2.9 ml/min./tube n Water 80 C 133 mbar.75 ml/min./tube Number of samples: 8 Tube used: , 600 ml tube, flat bottom, 75 mm diameter Block used: , 8 tube capacity Ending volume: dryness Vortex speed: 28% Vacuum pump displacement capacity: 88 liters/minute Maximum attainable vacuum: 31 mbar

11 RapidVap Vacuum Dry Evaporation Systems 11 Specifications Powder-coated aluminum base with acrylic/pvc thermoplastic upper housing. Glass lid with polycarbonate shield. Dual lid clamps. Phenol-free gasket. PTFE-coated aluminum chamber. Rear-mounted 1/2" OD vacuum port, 30" of 1/2" ID vacuum tubing, two tubing clamps and lid heater receptacle watt dry block heating system. Microprocessor-controlled programming for vortex speed from 0 to 100%; heat from OFF to 100 C; time from 1 to 999 minutes; vacuum level from 0 to 999 mbar. Memory stores 1 to 9 programs. LCD for display of program number and actual and set point vortex speeds, temperatures, time remaining, and point vacuum level. Control panel with RUN/STOP button, PREHEAT/END ALARM button and indicator light, VACUUM RELEASE button and indicator light, set point selection buttons, and increase/decrease programming buttons. Belt-driven, non-sparking, brushless DC motor with smooth variable-speed power to drive the vortex motion up to 1000 rpm. Audible alarm for timed end point. Audible/visual alarm for end point detected by temperature sensors at heater and block. Automatic vacuum release in the event of power failure. All mechanical components isolated from chemical fumes and vapors. 115 volt models include 6 1/2 foot, three-wire electrical cord with 20 amp plug. A 20 amp minimum circuit breaker is required. 230 volt models include 6 1/2 foot, three-wire electrical cord with 15 amp plug. A 10 amp minimum circuit breaker is required. ETL and ETL-C listing on all 115 volt, 60 Hz models. CE mark on all 230 volt, 50 Hz models. Overall dimensions with closed lid: 20.7" wide x 18.4" deep x 13.5" high (52.6 x 46.7 x 34.3 cm). Overall dimensions with open lid: 20.7" wide x 21.3" deep x 24.9" high (52.6 x 54.1 x 63.2 cm). Actual weight 92 lbs. (42 kg) Shipping weight 100 lbs. (45 kg) Made in U.S.A. Options Glass lid with 40-watt heater. RS-232 Link. RS-232 port, located on the rear of the unit, for 2-way communication at 4800 baud, no parity, 8 bit word, 1 stop bit and 3-wire interface. Accessories required (not included) Vacuum pump. See page 13. Sample block. See page 12. Glassware. See page 12 or contact your laboratory supply dealer. 115 volt, 20 amp plug 230 volt, 15 amp plug Ordering Information Catalog Number Electrical Specifications Lid Heater RS-232 Link volts, 50/60 Hz, 16 amps*** volts, 50/60 Hz, 8 amps volts, 50/60 Hz, 16 amps*** volts, 50/60 Hz, 8 amps volts, 50/60 Hz, 16 amps*** volts, 50/60 Hz, 8 amps volts, 50/60 Hz, 16 amps*** volts, 50/60 Hz, 8 amps International electrical configuration ***Amperage shown includes 8 amp maximum vacuum pump rating. Amperage shown includes 4.5 amp maximum vacuum pump rating. The RapidVap Vacuum Evaporation System should be located within a fume hood if hazardous or flammable solvents are used. In all cases, regardless of the solvent used, it is recommended that the vacuum pump be vented in a fume hood.

12 12 RapidVap Vacuum Dry Evaporation Systems Accessories PTFE-Coated Aluminum Blocks A block is required for all RapidVap Vacuum Evaporation Systems. Blocks are interchangeable. Custom blocks to fit special glassware such as VOA vials are available on request. Glassware is required (not included). See below for ordering information on Flat-Bottom Tubes for Blocks and For glassware for all other Blocks, contact your laboratory supply dealer. Catalog Block Sample Sample Shipping Number Tube Tube Volume Weight Capacity Sizes (ml) (lbs. kg) mm OD tubes, up to 6 ml* 4.5** 8/ mm OD tubes, up to 10 ml* 7.5** 8/ mm OD tubes, up to 23 ml* 17** 7.4/ ml conical centrifuge tubes, up to 15ml* 11** 7.8/ mm OD scintillation tubes, up to 50 ml* 34** 8.3/ ml borosilicate glass tubes 450** 6.4/ ml borosilicate glass tubes 125** 8.2/3.7 *Maximum tube height is 150 mm. **Actual sample volumes depend on tube shape, height and vortex speed. Flat-Bottom Tubes Borosilicate glass tubes offer better fit and heat transfer for faster evaporation rates. Catalog Sample Sample For Quantity per Shipping Number Tube Size (ml) Volume (ml) Block Package Weight 600 ml Flat-Bottom Tube and 170 ml Flat-Bottom Tube lb. (0.4 kg) lbs. (1.8 kg) lb. (0.4 kg) lbs. (1.8 kg) Eight-Place Stainless Steel Racks Catalog Sample Shipping Number Tube Size (ml) Weight Eight-Place Stainless Steel Racks and ml Tubes 2 lbs. (0.9 kg) ml Tubes 2 lbs. (0.9 kg) Glass Lid with Heater For applications involving water or other high boiling point solvents, a Lid Heater improves visibility and eliminates solvent condensation. The glass lid, with 40-watt heater bonded to it, plugs into the RapidVap s rear electrical receptacle. For 115 or 230 volt operation. Diaphragm Vacuum Pumps PTFE-coated wetted parts for corrosion resistance. Vacuum outlet is 1/2" OD. Diaphragm Vacuum Pump Catalog Electrical Vacuum Displacement Explosion- Shipping Number Specifications mbar Capacity Proof Weight (liters/minute) (lbs./kg) V, 60 Hz, 3.5 amps No 40/ * 230V, 50/60 Hz, 2.0 amps /63** No 40/18.1 * International electrical configuration. Power cord has reverse IEC plug. ** Displacement at 50/60 Hz Liquid Trap Liquid Trap Prevents liquid from entering the pump. Made of borosilicate glass. Trapping capacity is 2550 milliliters. Required accessory for diaphragm pump when using high boiling point solvents. Shipping weight 4 lbs. (1.8 kg).

13 RapidVap Vacuum Dry Evaporation Systems 13 Accessories Dry Ice Vacuum Traps When dry ice and solvent are added to the well, these secondary traps cool to approximately -75 C (-103 F). Inlet and outlet vacuum connections are 1/2" OD. Catalog Dimensions Well Volume of Ice trapping Liquid trapping Shipping Number (h x dia.) ice and alcohol capacity capacity Weight (liters) (ml) (liters) (lbs./kg) Dry Ice Vacuum Trap /4" x 7 7/8" (24.8 cm x 20 cm) / /8" x 6 5/8" (20 cm x 16.8 cm) /2.3 Vacuum Tubing Kit For use when connecting accessory traps to a vacuum system. Includes two each 1/2" ID vacuum tubing, 48" length, and 4 tubing clamps. Shipping weight 6 lbs. (2.7 kg). Trapping Valve Trapping Valve Aids in solvent recovery. Compatible with RapidVap Vacuum Systems manufactured after September Cold Trap is required (not included). See CentriVap Cold Traps below. Shipping weight 8 lbs. (3.6 kg). CentriVap -50 C Cold Trap CentriVap -84 C Cold Trap CentriVap -105 C Cold Trap CentriVap Cold Traps. See CentriVap CentriVap Centrifugal Concentrators and Cold Traps Catalog for complete information. Catalog Number Description Temperature Lid Electrical Requirements Receptacle & Plug Type Cold Trap -50 C (-58 F) Acrylic 115 volts, 60 Hz, 5.4 amps 115 volts, 60 Hz Cold Trap -50 C (-58 F) Stainless Steel 115 volts, 60 Hz, 5.4 amps 115 volts, 60 Hz * -50 Cold Trap -50 C (-58 F) Acrylic 230 volts, 50 Hz, 2.4 amps Schuko * -50 Cold Trap -50 C (-58 F) Stainless Steel 230 volts, 50 Hz, 2.4 amps Schuko Cold Trap -50 C (-58 F) Acrylic 230 volts, 60 Hz, 2.4 amps North America, 230 volts Cold Trap -50 C (-58 F) Stainless Steel 230 volts, 60 Hz, 2.4 amps North America, 230 volts * -50 Cold Trap -50 C (-58 F) Acrylic 230 volts, 50 Hz, 2.4 amps British (UK) * -50 Cold Trap -50 C (-50 F) Stainless Steel 230 volts, 50 Hz, 2.4 amps British (UK) * -50 Cold Trap -50 C (-58 F) Acrylic 230 volts, 50 Hz, 2.4 amps China/Australia * -50 Cold Trap -50 C (-58 F) Stainless Steel 230 volts, 50 Hz, 2.4 amps China/Australia Cold Trap -84 C (-119 F) Stainless Steel 115 volts, 60 Hz, 12 amps 115 volts, 60 Hz * -84 Cold Trap -84 C (-119 F) Stainless Steel 230 volts, 50 Hz, 6 amps Schuko Cold Trap -84 C (-119 F) Stainless Steel 230 volts, 60 Hz, 6 amps North America, 230 volts * -84 Cold Trap -84 C (-119 F) Stainless Steel 230 volts, 50 Hz, 6 amps British (UK) * -84 Cold Trap -84 C (-119 F) Stainless Steel 230 volts, 50 Hz, 6 amps China/Australia Cold Trap -105 C (-157 F) Stainless Steel 115 volts, 60 Hz, 12 amps 115 volts, 60 Hz * -105 Cold Trap -105 C (-157 F) Stainless Steel 230 volts, 50 Hz, 6 amps Schuko Cold Trap -105 C (-157 F) Stainless Steel 230 volts, 60 Hz, 6 amps North America, 230 volts * -105 Cold Trap -105 C (-157 F) Stainless Steel 230 volts, 50 Hz, 6 amps British (UK) * -105 Cold Trap -105 C (-157 F) Stainless Steel 230 volts, 50 Hz, 6 amps China/Australia

14 14 RapidVap N 2 and N 2 /48 Dry Evaporation Systems Phenol-free gasket Microprocessorcontrolled nitrogen blow down manifold Glass lid Dual lid clamps Easy-to-read LCD display Microprocessorcontrolled motor Unique Cool-Zone TM on RapidVap N 2 models PTFE-coated aluminum sample block Microprocessorcontrolled 1000-watt dry block heating system PTFE-coated aluminum chamber Made in U.S.A. Nitrogen blow down cluster on RapidVap N 2 /48 models

15 RapidVap N 2 and N 2 /48 Dry Evaporation Systems 15 Features and Benefits Fast evaporation rates, greater throughput. Vortex action, heat and nitrogen blow down combine to speed evaporation. Eight samples with volumes up to 450 ml each may be processed at once in the RapidVap N 2 and up to 48 samples with volumes up to 26 ml each in the RapidVap N 2 /48. See page 18 for evaporation rates. See pages for recovery rates. Microprocessor-controlled nitrogen blow down manifold. Allows user to introduce nitrogen to 2, 4, 6 or 8 individual sample tubes on the RapidVap N 2 or clusters of 6 on the RapidVap N 2 /48, conserving nitrogen when less than a full load is being processed. Nitrogen blown onto the surface of the sample helps facilitate the phase change from liquid to gas. Microprocessor-controlled 1000-watt dry block heating system. Speeds evaporation rate by supplying a controlled amount of heat from ambient up to a maximum of 100 C to the sample block. The heater and block move in tandem for more efficient heat transfer. Unlike water baths, the dry heating system adds no potential source of contamination and requires no maintenance. Microprocessor-controlled vortex motion increases surface area for faster evaporation. Vortex action continually mixes the sample, helps contain analytes in the solvent by constantly rinsing the tube walls, and maximizes sample recovery. Maintenance-free microprocessor-controlled motor. Reliable belt-driven system provides smooth variable-speed power to drive the vortex motion up to 500 rpm. Non-sparking, brushless DC motor ensures safety, control and reproducibility for protocols. PTFE-coated aluminum sample block. Provides efficient heat transfer, low maintenance and corrosion resistance. Block is easy to remove for cleaning or replacement. One 8-place block that holds 600 ml tubes is included with RapidVap N 2 models. Four accessory blocks holding 48 samples of various volumes are offered for the RapidVap N 2 /48 models. PTFE-coated aluminum chamber. Conducts heat efficiently and resists corrosion. Durable construction with sleek styling. The cabinet base is powder-coated cast aluminum. The upper housing is solvent-resistant, smooth acrylic/pvc thermoplastic. Glass lid. Provides visibility of samples, is chemical resistant and detaches for cleaning. Dual lid clamps. Two clamps hold the lid securely in place. Exclusive feature Phenol-free gasket. Provides a positive seal without potential for phenol contamination. Improved chemical compatibility. The operator may use a broad range of aggressive chemicals. Contact Labconco for specific chemical compatibility. Unique Cool-Zone on RapidVap N 2. Insulates sample remaining in glassware stem to permit processing to a desired end point. See page 17 for more information. Alarm signals completion of run allowing samples to be left unattended while evaporating to desired end point. The RapidVap N 2 and N 2 /48 have two means to signal completion of a run. With the first method, the operator sets the end point time and when set time has expired an audible alarm sounds and the RapidVap automatically turns off all functions. With the second method, the RapidVap monitors system temperature in the block and in the heater. During operation, evaporative cooling of the solvent creates a differential between the block and heater temperatures. Once evaporation is nearly complete, the two temperatures equalize indicating end point is near. The alarm sounds and the PREHEAT/END ALARM indicator light flashes. Convenient, easy-to-set programming. Parameters of time, heat, vortex speed and number of samples may be easily set with the touch of a few buttons. Up to 9 different user-set programs, each with different parameter set points, may be stored in memory. The user may alter parameters while a program is in progress. Easy-to-read LCD display. Prompts user to set program parameters and displays program number, number of active nitrogen positions, and actual and set point vortex speeds, temperatures, and time remaining. See details on page 16. All mechanical components are isolated from chemical fumes and vapors for longer life. ETL listed. All 115 volt models carry the ETL Testing Laboratories seal in the U.S. and ETL-C seal in Canada, signifying they meet or exceed all minimum requirements of UL Standard and CAN/CSA C22.2 No International electrical configurations available. All 230 volt models conform to the CE (European Community) requirements for electrical safety and electromagnetic compatibility. Reliability guarantee. Full one year warranty is provided against defects in materials and workmanship.

16 16 RapidVap N 2 and N 2 /48 Dry Evaporation Systems Control Panel Program parameter. LCD shows program from 1 to 9, R for run, or S for stop. Speed parameter. LCD shows vortex speed from 0 to 100%. Heat parameter. LCD shows heat from OFF to 99 ( C) or HI (100 C). Time parameter. LCD shows time selected from 1 to 999 minutes. During the run, the LCD shows time remaining. If RUN is selected instead of a time period, the RapidVap runs continuously. Sample parameter. LCD shows number of samples or clusters of samples selected to receive nitrogen blow down; 2, 4, 6 or 8 may be selected. Set point selection buttons. To select a parameter to change, press the set point select button directly below the parameter. LCD display. Prompts user to set program parameters and displays program number, actual and set point vortex speed by percentage, actual and set point temperature in C, time remaining in minutes, and number of active nitrogen positions. Preheat/End Alarm button with indicator light. Pressing the button activates the heater to begin elevating the block to set point temperature. When in preheat mode, the operator may monitor actual heat, adding samples and starting program once set point temperature has been reached. In addition, the indicator light flashes when end point has been detected by the temperature sensors. Pressing the button while the light is flashing switches the end point alarm from audible to muted or back to audible. Increase/Decrease buttons. Used to increase or decrease the last selected set point parameter. Run/Stop button. Initiates the start or stop of the selected program

17 RapidVap N 2 Dry Evaporation Systems 17 Rapid Evaporation Zone and Cool Zone This illustration demonstrates the effectiveness of the Rapid Evaporation Zone and the Cool-Zone in RapidVap N 2 models. In the Rapid Evaporation Zone, a 300 ml sample of methylene chloride is evaporated in 41 minutes at approximately 7.3 ml/ minute/tube (without cap). In the Cool-Zone with vortex motion, heat and nitrogen blow down still applied, methylene chloride is evaporated to a 1.0 ml end point in 9 minutes at a much slower rate, approximately 0.11 ml/minute/tube. If the RapidVap is programmed for timed end point, all functions automatically turnoff when time expires drastically slowing the evaporation rate. While samples are in the Cool-Zone, the user has ample time to retrieve samples or to perform solvent exchange procedures. *Time shown is with nitrogen blow down, vortex motion and heat still applied. Time would be longer if all functions were turned off.

18 18 RapidVap N 2 Dry Evaporation Systems Evaporation Rates The charts on this page indicate the evaporation times for the Rapid Evaporation Zone and the Cool-Zone. Times were determined with and without Glassware Caps over the sample tubes. Placing Glassware Caps over the sample tubes prevents contamination and prevents moisture from condensing in low boiling point solvents. The use of caps increases overall evaporation time 10 to 80 percent. See page 23 for Glassware Caps. RapidVap Evaporation Zone Rate Summary Solvent Evaporation Rate Evaporation Rate with cap without cap Water.79 ml/min./tube 1.1 ml/min./tube Methylene Chloride 5.1 ml/min./tube 7.3 ml/min./tube Ethyl Acetate 6.3 ml/min./tube 8.6 ml/min./tube Hexanes 10.3 ml/min./tube 14.3 ml/min./tube Acetone 5.6 ml/min./tube 6.4 ml/min./tube Toluene 5.0 ml/min./tube 6.7 ml/min./tube Number of samples: 8 Tube used: , 600 ml tube with 1.5 ml end point stem, 1.37" (3.5 cm) long Starting volume: 300 ml Ending volume: 1.0 ml Nitrogen pressure: 10 psi Vortex speed: 70% of maximum setting Rapid Evaporation Zone Cool-Zone * End point volume

19 RapidVap N 2 Dry Evaporation Systems 19 Specifications Powder-coated aluminum base with acrylic/pvc thermoplastic upper housing. Glass lid. Dual lid clamps. Phenol-free gasket. PTFE-coated aluminum chamber. PTFE-coated aluminum sample block capable of holding 8 each 600 ml tubes. Glassware sold separately watt dry block heating system. Microprocessor-controlled programming for vortex speed from 0 to 100%; heat from OFF to 100 C; time from 1 to 999 minutes; and number of active nitrogen positions, 2, 4, 6 or 8. Memory stores 1 to 9 programs. LCD for display of program number, actual and set point vortex speed, actual and set point temperature, time remaining, and number of active nitrogen positions. Memory stores 1 to 9 programs. Control panel with RUN/STOP button, PREHEAT/END ALARM button and indicator light, set point selection buttons, and increase/decrease programming buttons. Belt-driven, non-sparking, brushless DC motor with smooth variable-speed power to drive the vortex motion up to 500 rpm. Audible alarm with automatic shut down for timed end point. Audible/visual alarm for end point detected by temperature sensors at heater and block. Nitrogen inlet port supplied with a 1/4" male NPT thread. All mechanical components isolated from chemical fumes and vapors. Includes 8 polyethylene glassware caps, 6 feet of 2" dia. exhaust tubing, 78" of tubing for nitrogen supply and one tubing clamp. 115 volt models include 6 1/2 foot, three-wire electrical cord with 20 amp plug. A 15 amp minimum circuit breaker is required. 230 volt models include 6 1/2 foot, three-wire electrical cord with 15 amp plug. An 8 amp minimum circuit breaker is required. ETL and ETL-C listing on all 115 volt, 60 Hz models. CE mark on all 230 volt, 50 Hz models. Overall dimensions with closed lid: 21.7" wide x 19" deep x 13.5" high (55.1 x 48.3 x 34.3 cm). Overall dimensions with open lid: 21.7" wide x 21.3" deep x 24.9" high (55.1 x 54.1 x 63.2 cm). Actual weight 99 lbs. (45 kg) Shipping weight 107 lbs. (49 kg) Ordering Information Catalog Number Electrical Specifications RS-232 Link volts, 50/60 Hz, 9 amps * 230 volts, 50/60 Hz, 4.5 amps volts, 50/60 Hz, 9 amps * 230 volts, 50/60 Hz, 4.5 amps * International electrical configuration. Made in U.S.A. Option Glass lid with 40-watt heater. RS-232 Link. RS-232 port, located on the rear of the unit, for 2-way communication at 4800 baud, no parity, 8 bit word, 1 stop bit and 3-wire interface. Accessories required (not included) Glassware. See page 22. Nitrogen source with flow rate of 0.6 CFM/17 LPM minimum. See page 23 for ordering information on the Nitro 2LV Nitrogen Generator or contact your nitrogen gas supplier. Gas pressure regulator. Pressure of 5-10 psi is typical. Pressure must not exceed 20 psi. Contact a local supplier. 115 volt, 20 amp plug 230 volt, 15 amp plug The RapidVap N 2 Evaporation System should be located within a fume hood if hazardous or flammable solvents are used. In all cases, regardless of the solvent used, it is recommended that the exhaust hose be vented into a fume hood or other laboratory ventilation device.

20 20 RapidVap N 2 /48 Dry Evaporation Systems Evaporation Rates Toluene Acetonitrile Starting Volume 10 ml Time (min) Starting Volume (ml) Time (min) Methanol Hexane/ Ethyl Acetate 75:25 Hexane/ Ethyl Acetate 50:50 Number of samples: 48 Tube size: 20 mm Block used: Ending volume: dryness Nitrogen pressure: 20 psi Vortex speed: 100% Solvent Temperature Overall Evaporation Rate Methylene Chloride 50 C.59 ml/min./tube Toluene 80 C.43 ml/min./tube Acetonitrile 75 C.42 ml/min./tube Methanol 40 C.20 ml/min./tube Hexane/Ethyl Acetate 75:25 40 C.56 ml/min./tube Hexane/Ethyl Acetate 50:50 40 C.50 ml/min./tube Starting Volume 2 ml Number of samples: 48 Tube size: 12 mm Block used: Ending volume: dryness Nitrogen pressure: 8 psi Vortex speed: 100% Time (min) Block Preheat & Set Point Temperature Methylene Chloride Toluene Acetonitrile Methanol Hexane/ Hexane/ Ethyl Acetate Ethyl Acetate 75:25 50:50 40 C 80 C 75 C 40 C 40 C 40 C Solvent Temperature Overall Evaporation Rate Methylene Chloride 40 C.33 ml/min./tube Toluene 80 C.25 ml/min./tube Acetonitrile 75 C.25 ml/min./tube Methanol 40 C.11 ml/min./tube Hexane/Ethyl Acetate 75:25 40 C.40 ml/min./tube Hexane/Ethyl Acetate 50:50 40 C.40 ml/min./tube

21 RapidVap N 2 /48 Dry Evaporation Systems 21 Specifications Powder-coated aluminum base with acrylic/pvc thermoplastic upper housing. Glass lid. Dual lid clamps. Phenol-free gasket. PTFE-coated aluminum chamber watt dry block heating system. Microprocessor-controlled programming for vortex speed from 0 to 100%; heat from OFF to 100 C; time from 1 to 999 minutes; and number of active nitrogen clusters, 2, 4, 6 or 8. Memory stores 1 to 9 programs. LCD for display of program number, actual and set point vortex speed, actual and set point temperature, time remaining, and number of active nitrogen clusters. Control panel with RUN/STOP button, PREHEAT/END ALARM button and indicator light, set point selection buttons, and increase/decrease programming buttons. Belt-driven, non-sparking, brushless DC motor with smooth variable-speed power to drive the vortex motion up to 500 rpm. Audible alarm with automatic shut down for timed end point. Audible/visual alarm for end point detected by temperature sensors at heater and block. Nitrogen inlet hose, 1/4" ID. All mechanical components isolated from chemical fumes and vapors. Includes 6 feet of 2" dia. exhaust tubing, 78" of tubing for nitrogen supply and one tubing clamp. 115 volt models include 6 1/2 foot, three-wire electrical cord with 20 amp plug. A 15 amp minimum circuit breaker is required. 230 volt models include 6 1/2 foot, three-wire electrical cord with 15 amp plug. An 8 amp minimum circuit breaker is required. ETL and ETL-C listing on all 115 volt, 60 Hz models. CE mark on all 230 volt, 50 Hz models. Overall dimensions with closed lid: 21.7" wide x 19.0" deep x 13.5" high (55.1 x 48.3 x 34.3 cm). Overall dimensions with open lid: 21.7" wide x 21.3" deep x 24.9" high (55.1 x 54.1 x 63.2 cm). Actual weight 92 lbs. (42 kg) Shipping weight 100 lbs. (45 kg) The RapidVap N 2/48 Evaporation System should be located within a fume hood if hazardous or flammable solvents are used. In all cases, regardless of the solvent used, it is recommended that the exhaust hose be vented into a fume hood or other laboratory ventilation device. Made in U.S.A. Option RS-232 Link. RS-232 port, located on the rear of the unit, for 2-way communication at 4800 baud, no parity, 8 bit word, 1 stop bit and 3-wire interface. Accessories required (not included) Sample block. See page 22. Glassware. Contact your laboratory supply distributor. Nitrogen with flow rate of 3.5 CFM/100 LPM minimum. See page 23 for ordering information on the Nitro 2LV Nitrogen Generator or contact your nitrogen gas supplier. Gas pressure regulator. Pressure of 5-10 psi is typical. Pressure must not exceed 20 psi. Contact a local supplier. 115 volt, 20 amp plug 230 volt, 15 amp plug Ordering Information Catalog Number Electrical Specifications RS-232 Link volts, 50/60 Hz, 9 amps * 230 volts, 50/60 Hz, 4.5 amps volts, 50/60 Hz, 9 amps * 230 volts, 50/60 Hz, 4.5 amps * International electrical configuration.

22 22 RapidVap N 2 and N 2 /48 Dry Evaporation Systems Accessories PTFE-Coated Aluminum Blocks All RapidVap N2 Evaporation Systems include an 8-Place PTFE-Coated Aluminum Block Additional Blocks may be purchased for standby use. All RapidVap N 2 /48 Evaporation Systems require a Block (not included). Custom blocks to fit special glassware such as VOA vials are available upon request. Glassware is required (not included). Catalog Block Sample Sample For Use For Use Shipping Number Tube Tube Size Volume with with Weight Capacity (ml) N 2 N 2 /48 (lbs./kg) ml borosilicate glass tubes, up to 600 ml 450** 6.4/ ml borosilicate glass tubes, up to 170 ml 125** 8.2/ x 75 mm OD tubes, up to 6 ml 4.5** 8.0/ x 100 mm OD tubes, up to 10 ml 7.5** 8.0/ x 150 mm OD tubes, up to 23 ml 17** 7.4/ x 150 mm OD tubes, up to 35 ml 26** 7.8/3.5 *Maximum tube height is 150 mm. **Actual sample volumes depend on tube shape, height and vortex speed. 600 ml Tubes with Stems Borosilicate Glass Tubes For use with RapidVap N 2 Evaporation Systems only. The ground glass tubes offer better fit and heat transfer for faster evaporation rates. The stems are designed for use with Cool-Zone timed end point determinations. To minimize evaporation time, the tube with the correct end point stem should be used. For example, if an end point of 1.5 ml is desired, selecting Tube with a maximum 3.0 ml end point volume would result in an unnecessarily long period required to evaporate the final 1.5 milliliters in the stem. Flat-bottom tubes are for use in applications when samples are taken to dryness. Use the chart below to select the correct tube for your sample volume size and desired end point volume. Sample Desired End Point Tube Volume Volume Recommendations up to 125 ml Dryness , up to 125 ml 1.5 ml , up to 450 ml Dryness , up to 450 ml 1.5 ml , up to 450 ml ml , up to 450 ml ml , up to 450 ml ml , ml Tubes with Stems for Block Each graduated 600 ml tube has a sample capacity of up to 450 ml when vortexing. Catalog End Point Calibration Quantity per Shipping Number Volume Markings Package Weight ** 0.5 ml 0.5 ml 1 1 lb. (0.4 kg) ** 0.5 ml 0.5 ml 8 4 lbs. (1.8 kg) ** 1.5 ml 1.5 ml, 1.0 and 0.5 ml 1 1 lb. (0.4 kg) ** 1.5 ml 1.5 ml, 1.0 and 0.5 ml 8 4 lbs. (1.8 kg) ** 2.0 ml 2.0 and 1.0 ml 1 1 lb. (0.4 kg) ** 2.0 ml 2.0 and 1.0 ml 8 4 lbs. (1.8 kg) ** 3.0 ml 3.0, 2.0 and 1.0 ml 1 1 lb. (0.4 kg) ** 3.0 ml 3.0, 2.0 and 1.0 ml 8 4 lbs. (1.8 kg) **For use with RapidVap N 2 Evaporation Systems only

23 RapidVap N 2 and N 2 /48 Dry Evaporation Systems 23 Accessories 170 ml Tube with Stem 170 ml Tubes with Stems for Accessory Block Each graduated 170 ml tube has a sample capacity of up to 125 ml when vortexing. Catalog End Point Calibration Quantity per Shipping Number Volume Markings Package Weight * 0.5 ml 1.5 ml 1 1 lb. (0.4 kg) * 0.5 ml 1.5 ml 8 4 lbs. (1.8 kg) *For use with RapidVap N 2 Evaporation Systems only. Flat-Bottom Tubes Borosilicate glass tubes offer better fit and heat transfer for faster evaporation rates. Catalog Sample Sample For Quantity per Shipping Number Tube Size (ml) Volume (ml) Block Package Weight lb. (0.4 kg) lbs. (1.8 kg) * lb. (0.4 kg) * lbs. (1.8 kg) 600 ml Flat-Bottom Tube and 170 m,l Flat-Bottom Tube *For use with RapidVap N 2 Evaporation Systems only. Eight-Place Stainless Steel Racks Catalog Holds Shipping Number Weight ml Tubes 2 lbs. (0.9 kg) ml Tubes 2 lbs. (0.9 kg) Eight-Place Stainless Steel Racks and Glassware Caps Caps fit over Tubes described on page 18. Placing Caps on Tubes helps prevent cross contamination and moisture from condensing when using low boiling point solvents. Eight Polyethylene Glassware Caps are included with each RapidVap N 2 Evaporation System. Catalog Material Description Quantity per Shipping Number Package Weight Polyethylene caps * Polyethylene Fits 600 ml Tube lb. (0.2 kg) * PTFE Fits 600 ml Tube lb. (0.2 kg) * PTFE Fits 170 ml Tube lb. (0.2 kg) *For use with RapidVap N 2 Evaporation Systems only. NitroVap 2LV Nitrogen Gas Generator The NitroVap 2LV by Parker Hannifin produces up to 350 liters per minute of pure nitrogen at pressures up to 145 psi. With no electrical requirements, the NitroVap 2LV transforms standard compressed air into nitrogen and is ideal for use with RapidVap Evaporators. A supply of compressed air is required with a minimum flow rate of 335 liters per minute (11.8 scfm) at 50 psi and maximum flow rate of 660 liters per minute (23.3 scfm) at 150 psi. *For use with RapidVap N2, N 2 /48 and Vertex Evaporation Systems only. NitroVap 2LV Nitrogen Gas Generator

24 24 RapidVap Vertex System Recovery Data Test results show that the RapidVap Vertex Evaporation System produces excellent recoveries of a wide range of compounds. Procedure: A 15 milliliter sample consisting of a combination of several of the compounds listed below was prepared in duplicate along with a method blank of pure solvent and each sample or blank was added to a 20 x 150 mm tube and placed in Block BNA mixtures were added to Methylene Chloride and run at 40 C and 12 psi nitrogen for approximately 30 minutes. A second set of samples was run at 40 C and 24 psi nitrogen for approximately 22 minutes. All other compound mixtures were added to Hexane and run at 70 C and 12 psi nitrogen for approximately 15 minutes. A second set of samples was run at 24 psi nitrogen for approximately 12 minutes. Ending volumes for all samples were approximately 1 milliliter. Recovery rates for the two samples were averaged and are shown below. BNAs (EPA psi N-Nitrosodimethylamine Phenol Aniline Bis(2-Chloroethyl) Ether Chlorophenol ,3-Dichlorobenzene ,4-Dichlorobenzene Benzyl Alcohol ,2-Dichlorobenzene Methylphenol Bis(2-Chloroisopropyl) Ether n-nitroso-di-n-propylamine (3 & 4)-Methylphenol Hexachloroethane Nitrobenzene Isophorone Nitrophenol ,4-Dimethylphenol Bis(2-Chloroethoxy)Methane ,4-Dichlorophenol ,2,4-Trichlorobenzene Naphthalene Chloroaniline Hexachlorobutadiene Chloro-3-methylphenol Methylnaphthalene Hexachlorocyclopentadiene ,4,6-Trichlorophenol ,4,5-Trichlorophenol Chloronaphthalene Nitroaniline Dimethylphthalate Acenaphthylene ,6-Dinitrotoluene Nitroaniline Acenaphthene ,4-Dinitrophenol Dibenzofuran ,4-Dinitrotoluene Nitrophenol Diethyl Phthalate Fluorene Chlorophenyl Phenyl Ether Nitroaniline ,6-Dinitro-2-methylphenol N-Nitrosodiphenylamine Azobenzene Bromophenyl Phenyl Ether Hexachlorobenzene Pentachlorophenol Phenanthrene Anthracene Di-n-butyl Phthalate Fluoranthene Pyrene Butyl Benzyl Phthalate Benzo[a]anthracene Chrysene Bis[2-Ethylhexyl] Phthalate Di-n-octyl Phthalate Indeno[1,2,3-cd]Pyrene Benzo[b]Fluoranthene Benzo[k]Fluoranthene Benzo[a]Pyrene Dibenz[a,h]anthracene Benzo(ghi)perylene Organochlorine Insecticides & Metabolites (EPA psi alpha-chlordane gamma-chlordane alpha-bhc gamma-bhc (Lindane) beta-bhc Heptachlor delta-bhc Aldrin Heptachlor Epoxide Endosulfan I ,4 -DDE Dieldrin Endrin ,4 -DDD Endosulfan II ,4 -DDT Endrin Aldehyde Endosulfan Sulfate Methoxychlor Endrin ketone Hexachlorobenzene Organophosphorus Insecticides (EPA psi Naled Dichlorvos Mevinphos Ethoprop Phorate Demeton, O & S Diazinon Disulfoton Dimethoate Ronnel Chlorpyrifos Methyl Parathion Fenthion Malathion Merphos Tokuthion (Prothiofos) Bolstar Fensulfothion Methyl Azinphos Coumaphos EPN Ethyl parathion Sulfotepp Tetrachlorvinphos Nitrogen/Phosphorus Herbicides & Insecticides (EPA psi EPTC Butylate Propachlor Trifluralin Terbufos Atrazine Simazine Alachlor Metribuzin Metolachlor Pendimethalin Butachlor Cyanazine Acetochlor Chlorinated Phenoxy Herbicides (EPA psi Dalapon ,5-Dichlorobenzoic acid Dicamba Dichlorprop ,4-D Pentachlorophenol ,4,5-TP (Silvex) Chloramben ,4,5-T ,4-DB Bentazon Picloram Dinoseb DCPA Acifluorfen

25 RapidVap Vacuum System Recovery Data 25 Test results show that the RapidVap Vacuum Evaporation System produces excellent recoveries of a wide range of compounds. Procedure: Samples consisting of a combination of several of the compounds listed below were prepared and each of the resulting compound mixtures was added to a separate 600 milliliter flat-bottom sample tube and placed in Sample Block in the RapidVap The following parameters were set: heat at 50 C, vortex speed at 28% of maximum, and vacuum of 250 mbar. The RapidVap was allowed to run continuously until the sample volume was reduced to 1 ml. A mass spectrometer was used to compare original compound amounts to the amounts detected in the reduced sample. Duplicates of the compound mixtures were tested and the percentages averaged. BNAs Compound Acenaphthene 79 Acenaphthylene 80 Aniline 64 Anthracene 86 Azobenzene 84 Benzo(a)anthracene 82 Benzo(a)Pyrene 85 Benzo(b)Fluoranthene 84 Benzo(g,h,i)perylene 86 Benzo(k)Fluoranthene 87 Benzoic Acid 70 Benzyl Alcohol 73 Bis(2-Chloroethoxy) Methane 75 Bis(2-Chloroethyl) Ether 67 Bis(2-Chloroisopropyl) Ether 67 Bis(2-ethylhexyl)Phthalate 88 4-Bromophenyl Phenyl Ether 76 Butyl Benzyl Phthalate 85 4-Chloro-3-methylphenol 78 4-Chloroaniline 90 2-Chloronaphthalene 75 2-Chlorophenol 64 4-Chlorophenyl Phenyl Ether 80 Chrysene 86 Dalapon 58 Di-n-butyl Phthalate 86 Di-n-octyl Phthalate 89 Dibenzo(a,h)anthracene 84 Dibenzofuran 82 1,2-Dichlorobenzene 63 1,3-Dichlorobenzene 62 1,4-Dichlorobenzene 62 3,3 -Dichlorobenzidine 86 2,4-Dichlorophenol 72 Diethyl Phthalate 82 2,4-Dimethylphenol 68 Dimethylphthalate 79 4,6-Dinitro-2-methylphenol 81 2,4-Dinitrophenol 70 2,4-Dinitrotoluene 80 2,6-Dinitrotoluene 83 Fluoranthene 85 Fluorene 83 2-Fluorobiphenyl (SURR) 75 2-Fluorophenol (SURR) 46 Hexachlorobenzene 84 Hexachlorobutadiene 67 Hexachlorocyclopentadiene 58 Hexachloroethane 62 Indeno(1,2,3-cd)Pyrene 84 Isophorone 76 (3 & 4)-Methylphenol 73 2-Methylnaphthalene 78 2-Methylphenol 69 n-nitroso-di-n-propylamine 90 BNAs (continued) Compound N-Nitrosodimethylamine 63 N-Nitrosodiphenylamine 82 Naphthalene 71 2-Nitroaniline 79 3-Nitroaniline 86 4-Nitroaniline 82 Nitrobenzene 67 Nitrobenzene-d5 (SURR) 62 2-Nitrophenol 69 4-Nitrophenol 87 p-terphenyl-d14 (SURR) 84 Pentachlorophenol 71 Phenanthrene 86 Phenol 69 Phenol-d6 (SURR) 64 Pyrene 81 Pyridine 59 1,2,4-Trichlorobenzene 69 2,4,5-Trichlorophenol 75 2,4,6-Tribromophenol (SURR) 74 2,4,6-Trichlorophenol 76 Chlorinated Herbicides (Phenoxys) Analyte Bentazon 95 Chloramben 116 2,4-D 94 2,4-DB 99 Dicamba 100 3,5-Dichlorobenzoic Acid 91 Dichlorprop 89 MCPA 80 MCPP 86 Pentachlorophenol 68 Picloram 106 2,4,5-T 118 2,4,5-TP (Silvex) 92 Tetrachloroterephthalic Acid 90 Organophosphorus Pesticides Analyte Bolstar 85 Chlorpyrifos 83 Coumaphos 90 Demeton, O & S 107 Diazinon 86 Dichlorvos 86 Dimethoate 123 Disulfoton 71 EPN 87 Ethoprofos 89 Ethoprop 81 Ethyl Parathion 91 Fensulfothion 94 Organophosphorus Pesticides (continued) Compound Fenthion 77 Malathion 87 Merphos 84 Methyl Azinphos 92 Methyl Parathion 85 Mevinphos 110 Monocrotophos 99 Naled 78 Phorate 75 Ronnel 79 Sulfotepp 82 TEPP 95 Tokuthion* 79 Trichloronate 80 Semivolatile Organic Pesticides Analyte Aldrin 83 alpha-bhc 88 beta-bhc 100 4,4 -DDD 87 4,4 -DDE 83 4,4 -DDT 93 delta-bhc 96 Dieldrin 83 Endosulfan I 75 Endosulfan II 84 Endosulfan sulfate 93 Endrin 90 Endrin Ketone 96 gamma-bhc (Lindane) 100 Heptachlor 83 Heptachlor epoxide 79 Methoxychlor 97 Testing conducted by Keystone Laboratories, Inc. Newton, Iowa

26 26 RapidVap N 2 System Recovery Data Test results show that the RapidVap N 2 Evaporation System produces excellent recoveries of a wide range of compounds. Procedure: Samples consisting of a combination of several of the compounds listed below were prepared and each of the resulting compound mixtures was added to a separate 600 milliliter sample tube with 1.5 milliliter stems containing 300 ml methylene chloride and placed in the RapidVap Sample tubes were not capped. The following parameters were set: heat at 40 C, vortex speed at 60% of maximum, and nitrogen pressure of 10 psi. The RapidVap was allowed to run continuously until the sample volumes were reduced to 1 ml. A mass spectrometer was used to compare original compound amounts to the amounts detected in the reduced samples. Duplicates of the compound mixtures were tested and the percentages averaged. BNAs Analyte Acenaphthene 97 Acenaphthylene 92 Aniline 87 Anthracene 91 Azobenzene 83 Benzo(a)anthracene 97 Benzo(a)Pyrene 91 Benzo(b)Fluoranthene 96 Benzo(g,h,i)perylene 82 Benzo(k)Fluoranthene 100 Benzoic Acid 100 Benzyl Alcohol 80 Bis(2-Chloroethoxy) Methane 81 Bis(2-Chloroethyl) Ether 76 Bis(2-Chloroisopropyl) Ether 67 Bis(2-ethylhexyl)Phthalate 93 4-Bromophenyl Phenyl Ether 86 Butyl Benzyl Phthalate 94 4-Chloro-3-methylphenol 91 4-Chloroaniline 99 2-Chloronaphthalene 76 2-Chlorophenol 95 4-Chlorophenyl Phenyl Ether 82 Chrysene 96 Di-n-butyl Phthalate 87 Di-n-octyl Phthalate 90 Dibenzo(a,h)anthracene 92 Dibenzofuran 78 1,2-Dichlorobenzene 78 1,3-Dichlorobenzene 75 1,4-Dichlorobenzene 75 3,3 -Dichlorobenzidine 97 2,4-Dichlorophenol 97 Diethyl Phthalate 85 2,4-Dimethylphenol 100 Dimethylphthalate 80 4,6-Dinitro-2-methylphenol 100 2,4-Dinitrophenol 100 2,4-Dinitrotoluene 82 2,6-Dinitrotoluene 83 Fluoranthene 100 Fluorene 97 2-Fluorobiphenyl (SURR) 79 2-Fluorophenol (SURR) 77 Hexachlorobenzene 84 Hexachlorobutadiene 78 Hexachlorocyclopentadiene 73 Hexachloroethane 71 Indeno(1,2,3-cd)Pyrene 86 Isophorone 77 2-Methylnaphthalene 83 (3 & 4)-Methylphenol Methylphenol 100 n-nitroso-di-n-propylamine 73 N-Nitrosodimethylamine BNAs (continued) Analyte N-Nitrosodiphenylamine 85 Naphthalene 94 2-Nitroaniline 85 3-Nitroaniline 92 4-Nitroaniline 95 Nitrobenzene 77 Nitrobenzene-d5 (SURR) 77 2-Nitrophenol 98 4-Nitrophenol 100 p-terphenyl-d14 (SURR) 87 Pentachlorophenol 100 Phenanthrene 91 Phenol 95 Phenol-d6 (SURR) 76 Pyrene 96 Pyridine 6 2,4,6-Tribromophenol (SURR) 79 2,4,5-Trichlorophenol 94 2,4,6-Trichlorophenol 96 Chlorinated Herbicides (Phenoxys) Analyte Acifluorfen 100 Bentazon 93 Chloramben 46 2,4-D 100 2,4-DB 93 Dicamba 87 3,5-Dichlorobenzoic Acid 83 Dichlorprop 82 4-Nitrophenol 100 MCPA 80 MCPP 78 Pentachlorophenol 82 2,4,5-T 78 2,4,5-TP (Silvex) 85 Organophosphorous Pesticides Analyte Bolstar 89 Chlorpyrifos 86 Coumaphos 98 Demeton, O & S 82 Diazinon 89 Dichlorvos 83 Disulfoton 72 EPN 88 Ethoprop 88 Ethyl Parathion 86 Fensulfothion 100 Fenthion 89 Malathion 92 Merphos 93 Methyl Azinphos 99 Methyl Parathion 84 Organophosphorus Pesticides (continued) Analyte Mevinphos 88 Monocrotophos 82 Naled 83 Phorate 79 Ronnel 85 TEPP 59 Tokuthion 88 Trichloronate 85 Semivolatile Organic Pesticides Analyte Aldrin 84 alpha-bhc 88 beta-bhc 86 4,4 -DDD 90 4,4 -DDE 86 4,4 -DDT 96 delta-bhc 92 Dieldrin 87 Endosulfan I 92 Endosulfan II 93 Endosulfan sulfate 87 Endrin 92 gamma-bhc (Lindane) 84 Heptachlor 84 Heptachlor epoxide 92 Methoxychlor 96 Testing conducted by Keystone Laboratories, Inc. Newton, Iowa

27 RapidVap N 2 /48 System Recovery Data 27 Tests results show that the RapidVap N 2 /48 Evaporation System produces excellent recoveries of a wide range of analytes. Carryover from one tube to another was not detectable. Procedure: Samples consisting of a combination of several of the analytes listed below were prepared and each of the resulting analyte mixtures was added to a separate 20 mm sample tube containing 10 ml methylene chloride and placed in the RapidVap N 2 / The following parameters were set: heat at 40 C, vortex speed at 100% of maximum, and nitrogen pressure of 15 psi. The RapidVap was allowed to run continuously until the sample volumes were reduced to 1 ml. A mass spectrometer was used to compare original compound amounts to the amounts detected in the reduced samples. Duplicates of the analyte mixtures were tested and the percentages averaged. BNAs Analyte 1,2,4-Trichlorobenzene ,2-Dichlorobenzene ,3-Dichlorobenzene ,4-Dichlorobenzene ,4,5-Trichlorophenol ,4,6-Tribromophenol (SURR) ,4,6-Trichlorophenol ,4-Dichlorophenol ,4-Dimethylphenol ,4-Dinitrophenol ,4-Dinitrotoluene ,6-Dinitrotoluene Chloronaphthalene Chlorophenol Fluorobiphenyl (SURR) Fluorophenol (SURR) Methylnaphthalene Methylphenol Nitroaniline Nitrophenol & 4-Methylphenol ,3-Dichlorobenzidine Nitroaniline ,6-Dinitro-2-Methylphenol Bromophenyl Phenyl Ether Chloro-3-Methylphenol Chloroaniline Chlorophenyl-Phenyl Ether Nitroaniline Nitrophenol 95.5 Acenaphthene 95.6 Acenaphthylene 96.8 Aniline 88.8 Anthracene 96.1 Azobenzene 94.2 Benzidine 96.0 Benzo(a) Pyrene 95.1 Benzo(a) Anthracene 97.7 Benzo(b) Fluoranthene 96.4 Benzo(g,h,i) Perylene 88.3 Benzo(k) Fluoranthene 95.8 Benzoic Acid 87.2 Benzyl Alcohol 91.5 Bis-(2-Chloroethoxy) Methane 92.5 Bis(2-Chloroethyl) Ether 83.2 Bis(2-Chloroisopropyl) Ether 84.6 Bis(2-Ethylhexyl) Phthalate 97.8 Butyl Benzyl Phthalate 97.7 Carbazole Chrysene 99.0 Dibenzo(a,h) Anthracene 88.2 BNAs (continued) Analyte Dibenzofuran 95.0 Diethyl Phthalate 96.7 Dimethyl Phthalate 96.5 Di-n-butyl Phthalate 98.4 Di-n-octyl Phthalate 95.9 Fluoranthene 98.1 Fluorene 96.7 Hexachlorobenzene 95.9 Hexachlorobutadiene 84.0 Hexachlorocyclopentadiene 90.3 Hexachloroethane 80.2 Indeno(1,2,3-cd) Pyrene 87.9 Isophorone 94.7 Naphthalene 88.7 Nitrobenzene 87.9 Nitrobenzene-d5 (SURR) 87.8 N-Nitrosodimethylamine 75.9 N-Nitroso-Di-n-propylamine 91.7 N-Nitrosodiphenylamine Pentachlorophenol 93.7 Phenanthrene 96.9 Phenol 84.5 Phenol-d6 (SURR) 87.3 p-terphenyl-d14 (SURR) 98.1 Pyrene 96.9 Pyridine 74.5 Chlorinated Herbicides (Phenoxys) Analyte 2,4,5-T 100 2,4-D 100 2,4-DB 53 3,5-Dichlorobenzoic Acid Nitrophenol 70 Acifluorfen 100 Bentazon 100 Chloramben 100 Dalapon 89 Dicamba 100 Dichloroprop 100 Dinoseb 100 MCPA 100 MCPP 100 Pentachlorophenol 100 Picloram 96 Silvex 100 Organophosphorous Pesticides Analyte Chlorpyrifos 98.6 Coumaphos 99.8 Demeton 90.7 Diazinon 97.4 Dichlorvos 94.2 Disulfoton 99.9 EPN 94.8 Ethoprop 98.8 Fensulfothion 75.9 Fenthion 97.8 Malathion 89.2 Merphos 95.5 Methyl Parathion Mevinphos 94.8 Monocrotophos 87.9 Naled 99.9 Phorate 98.4 Ronnel 98.0 TEPP 63.4 Tokuthion Trichloronate 98.1 Semivolatile Organic Pesticides Analyte 4,4-DDD ,4-DDE ,4-DDT 99.7 Aldrin 97.9 alpha-bhc 97.4 beta-bhc 98.1 delta-bhc 96.8 Dieldrin 96.3 Endosulfan I 97.0 Endosulfan II 98.7 Endosulfan Sulfate 99.6 Endrin 99.0 Endrin Aldehyde 97.0 Endrin Ketone 98.0 gamma-bhc 96.1 Heptachlor 97.5 Heptachlor Epoxide 99.4 Methoxychlor 96.6 Testing conducted by Keystone Laboratories, Inc. Newton, Iowa

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