BENCHTOP FERMENTOR & BIOREACTOR SYSTEMS
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1 BENCHTOP FERMENTOR & BIOREACTOR SYSTEMS A SELECT NEW RANGE OF VERSATILE, HIGH PERFORMANCE 3-20L AUTOCLAVABLE SYSTEMS WITH EASY-TO-USE TOUCHSCREEN CONTROLS c.com
2 ...Introducing a new player in bench top fermentors and bioreactors! Cleaver Scientific is proud to launch an exciting new range of high performance fermentors and bioreactors. Remarkably user-friendly and equipped with intuitive controls, these fermentors and bioreactors are suitable for most fermentation and cell culture applications performed by: University teaching labs; Life science research labs; R&D facilities within biofuel and biotech companies; Pharmaceutical organisations; Food and beverage manufacturers. Each system is supplied as a customised solution with vessel, impeller, control station and probes for growth of different microbes, yeast, mammalian, insect and plant cells, removing the complexities often faced with other brands of equipment selection and set up. Assembly is straight forward with the unit being unpacked and made ready for the autoclave in less than an hour. The control station is fully integrated with built-in peristaltic pumps, gas flow control, and ph, dissolved oxygen (DO), substrate feed and antifoam functionalities all installed in one bench top unit. An easy-to-use large 10.4-inch colour touchscreen interface allows setting and real-time pictorial display of agitation speed, temperature, ph and dissolved oxygen (DO), while 4 assignable built-in peristaltic pumps act in tandem with the corresponding probe sensor to control ph, DO and antifoam feeds. Each probe is connected to an easily accessible side panel that also houses quick-fit connectors for rapid set up of the heating system and ancillary equipment for aeration and cooling. 2
3 Changing with your needs ¹ ² ³ A wide range of accessories, including l multiple probe options, ensure easy customisation of each bench top fermentation and cell culture system for many different cell types and microbes. From left to right, probes include: (1.) ph, (2.) DO, (3.) temperature, (4.) antifoam and (5.) oxidoreduction potential (ORP). 4 5 Grow almost any cell type With a wide range of vessels and impellers, including customised options, Cleaver Scientific fermentation and bioreactor systems have the capacity to grow virtually any cell type: whether it s anaerobic or aerobic microbes, yeast, insect, plant or mammalian cells. Pre-programmed for fermentation & cell culture All Cleaver Scientific fermentors and reactors are pre-programmed with fermentation and cell culture operating modes allowing each system to adapt as your needs change. Different vessel configurations All five types of interchangeable single-wall, double-wall, heat-blanketed, jacketed and air lifter vessels are available in 3 to 20 litre volumes and made from autoclavable borosilicate. Multiple options & accessories Full adaptability is assured with up to 4 probe options for ph, DO and temperature; 4 brushless motor drives with 12 choices of impeller; 2 adjustable flow meters, and 5 vessel types in as many as 6 different volumes. Simple to use Touch-and-grow A large 10.4 colour touch screen interface built within the control station allows the user to control and view each parameter within a conspicuous, easy-toread pictorial display. ¹ ² A low maintenance, brushless 24V DC motor (1.) is available as standard with each system and may be used with both types of standard impeller, such as the Rushton-style, as shown (2.), for fermentation, or the low-shear pitched blade impeller (3.) for more fragile insect, plant and animal cultures. Bespoke impellers are available on request for full flexibility. ³ Helpful screen tabs The colour touch screen is pre-configured for use with the selected reactor vessel, while 8 different screen TABS facilitate user-fiendly control of all critical process values within a single screen (see page 5). Same software on all units The same control software features on all fermentors and bioreactors allowing the user to change to other Cleaver Scientific systems when required, while ethernet remote control is available without additional software. upgrades. Automatic s Gas flow control Automatic multi-gas mixing options - up to two and four respectively in fermentation and cell culture mode - ensure optmised yields and cell growth, while a selection of customisable gas flow options provide complete flexibility by allowing customers to design systems specific to their needs. Adjustable air flow Each system features as standard an adjustable air flow rotameter on the front of the control station. An optional oxygen (O 2 ) enrichment module comprising adjustable rotameter and O 2 enrichment valve may also be fitted. Bespoke thermal mass flow control (TMFC) options are available on request. 2-stage cascade with P-I-D technology A built-in 2-stage cascade feature utilises P-I-D (Proportional-Integral-Derivative) control technology to maintain the DO setpoint through automatic adjustment of agitation speed and substrate feeds, and optional O 2 enrichment. The adjustable P-I-D values for ph and DO may be defined automatically by the size of the reactor vessel, or after manual fine-tuning by the user. Choose glass-walled vessels as shown or heat-blanketed vessels. Customise your system with up to two rotameters or optional TMFC for gas overlay and sparge. Leave it to its own devices P-I-D control of each setpoint obviates the need for constant operator supervision, thus affording the user the time to perform other tasks at their convenience while the system remains unattended. 3
4 'Get touching and start growing!' Simple to use touchscreen interface facilitates set up in less than an hour. No configuration needed. Station and s Low maintenance rpm brushless motor available for all models. Supplied with 'quick' connector for easy vessel connection. Optional low 5-300rpm motor recommended for cell culture. Side panel provides convenient access to all connections for probe sensors and motor, as well as the sparger, cooling system and condenser inch colour touchscreen interface included as standard with all units. Helpful screen tabs guide you through each step of the set-up process. 'Get touching and start growing!' Heat-blanketed dish bottom vessels available in 6 sizes, ranging from 3 to 20L. Larger 15L and 20L vessels are ideal for process scale-up as cost-effective alternatives to expensive sterilisable-inplace systems (SIP). Dry-heating blanket suitable for growth of microbes and non-photosynthetic plant cells. Single-wall plain bottom vessel with heating base available in 4 sizes: 3, 5, 7.5 and 10L. Dry-heating base ensures rapid heating and cooling. Perfect entrylevel system for microbial fermentation. Double-wall round bottom vessels available in 4 sizes: 3, 5, 7.5 and 10L. Warm or cool water circulates through the outer wall cavity for thermostatic temperature control. Especially suitable for growth of mammalian, microbial and insect cells. Four peristaltic pumps may be linked directly to acid, base, antifoam and substrate feeds. Pump speed adjustable within 1rpm increments. Up to two rotameters one for gas flow control with optional second rotameter for O2 enrichment. Stop-Start convenient front access. Small footprint 40x50cm (WxD) - allows control station to fit most benches comfortably. Side Panel 4 panel with connection ports for ph, ORP, DO, antifoam and vessel temperature probes; gas mixing station and motor; and dry-heating base unit / blanket and blanket temperature probe (on CSL FS-02 models only). Air and cooling panel with connection ports for condenser cooling and vessel cooling-coil water inlets and outlets; condenser flow regulator; main water inlet and outlet drain; gas outlet to sparger; and air and O2 inlets. USB port for data export to USB flash drive or computer. Remote control and system expansion panel includes extension ports, RE-485 connection for ancillary fifth peristaltic pump, power socket and ethernet port for connection to optional laptop shown. ¹ ² Air-lifter vessels available in doublejacketed (1) and single-wall (2) formats - facilitate unique impeller-free growth of fragile plant and fungal cells using microsparger and adjustable draft tube.
5 Headplate Ancillary Pump Single-wall round bottom vessels available in 4 sizes: 3, 5, 7.5 and 10L. Inner circulation loop facilitates rapid heating and cooling. Ideal for anaerobic and aerobobic fermentation. Threaded penetrations in 316L stainless steel headplate provided for probes; acid base and substrate feeding ports; septum port for small volume injection (e.g. antibiotics); condenser to prevent culture media evaporation; sparger, cooling ports, and inoculation and sampling ports. T-shaped hand-lifters for convenient vessel transfer and manoeuvrability. External peristaltic pumps may be connected to control station by RE- 485 port. Fifth external pump may be controlled in the PUMP screen tab (see sample screen 2 below). Pump head is reversible and may be used with any one of six silicon tubes, ranging from 0.8 to 7.9mm in internal diameter, depending on the desired flow rate (36 to 2300 ml/min). Silicon tubes supplied in 7.6m lengths. Sample Screens 1. MONITOR - shows a full summary of all critical processes within one easy-to-read screen, including: operation status; setpoint and present value of each parameter; user login and working status of antifoam, ph and substrate feeds; process time and manual or automatic operation. 2. PUMP - for simultaneous control, calibration and assignment of all 4 peristaltic pumps, plus fifth ancillary pump if used. 3. CONTROL - performs manual or automatic control of ph, agitation, temperature and DO setpoints. 4. SETTING - allows selection of the program schedule for each automatic process. 5. CHART - shows real time recording of trend data for each fermentation or cell culture process 6. CALIBRATION -for accurate calibration of each probe sensor. 7. MAINTENANCE - to set up a service and maintenance schedule. 8. HELP - for set up and operation assistance, particularly the cooling and air system following sterilisation. Each screen may be viewed and programmed using ethernet remote control with optional lap top. 5
6 TECHNICAL SPECIFICATION * Thermostatic Bioreactor and Fermentation Systems (CSLFS-01) Double-wall round bottom vessel systems Single-wall round bottom vessel systems Air-lifter vessel systems Double-wall Single-wall Series CSLFS-V-A CSLFS-V-B CSLFS-V-C VESSEL CONTROL STATION TEMPERATURE AGITATION AERATION ph DO ANTIFOAM EXHAUST Working volume 3L 5L 7L 10L 3L 5L 7L 10L 5L Total volume 3.7L 6.8L 8.9L 12.5L 3.7L 6.8L 8.9L 12.5L 6.9L Design All vessels comprise autoclavable borosilicate glass contruction with 316L stainless steel headplate with fittings Design features (A) Compact control station with integrated controller including built-in pumps and side panel capable of supporting utilities; (B) storage for 59,994 process programmes; (C) log data storage for 10 process monitoring files; (D) real-time trend data recording; (E) industrial standard ABS front panel with painted iron housing Display 10.4-inch (265mm) colour touchscreen interface supplied as standard with each control station Function Fermentation and cell culture monitoring and control Dimensions Footprint (W x D): 400 x 500mm (15.75 x 19.69"); Height: 740mm (29.13") Probe 5 C above coolant to maximum 60 C (A) Thermostatic system with programmable 15-step PID control; (B) built-in heat exchanger (400W heater / water circulation pump); (C) automatic cooling water valve Double-wall water jacket heater and circulation loop (see B and C above) Platinum RTD probe (Pt100) Single-wall vessel with inner coil heater and circulation loop (see B and C above) Double-wall water jacket heater and circulation loop (see B and C above); no temperature control for singlewall vessels Drive Removable top 'brushless' motor n/a rpm standard motor for routine fermentation and cell culture; 5-300rpm option for shear sensitive cell lines n/a Manual setting or programmable 15-step PID control n/a Impellers 2 pieces of adjustable pitched-blade impeller for fragile cell types 3 pieces of adjustable Rushton-style impeller for less shear-sensitive microbial cell types Baffles Removable 316L stainless steel baffle for fermentation n/a Gas flow options Standard built-in air-flow rotameter (1-10Lpm for 3/5L or 2-20Lpm for 7/10L vessels); optional O2 enrichment module with rotameter and enrichment valve (bespoke TMFC possible - please enquire) Gas mixing Manual / automatic air and O2 mixing; additional 4-gas mixing station available Sparger Orifice ring sparger supplied as standard Micro-sparger Inlet filter 0.2μM absolute filter Filter Condenser 2-14 ph; 0.01pH resolution PID, linked to acid/base pumps; one-point control with adjustable deadband (ph control range) Gel-filled, autoclavable ph probe 0-200%; 0.1% resolution n/a Standard built-in air-flow rotameter (1-10Lpm for 5L vessels); adjustable draft tube; O2 enrichment optional PID, 2-stage cascade: starting with agitation (1), cascading to optional O2 enrichment (2) if agitation speed is maximum but DO is below setpoint; substrate feeding programme (3) activated at DO setpoint Autoclavable, polarographic DO probe On/off 316L stainless steel with insulated Teflon tube 0.2μM absolute filter 316L stainless steel PUMPS 4 built-in, assignable, with manual setting or programmable 15-step PID control; 0-65rpm (RE-485 connection on control station for fifth pump option) UTILITIES Water / gas / power 2 bar maximum (29 PSI) / 1 bar maximum (14.5 PSI); dry and oil-free / V 50/60Hz or V 50/60Hz; electrical safety cut-off switch *Specifications may be subject to change without notice 6
7 TECHNICAL SPECIFICATION * Dry-Heating Bioreactor and Fermentation Systems (CSLFS-02) Heat-blanketed single-wall round bottom vessel systems Single-wall plain bottom vessel systems with heating base Series CSLFS-V-B CSLFS-V-D VESSEL CONTROL STATION TEMPERATURE AGITATION AERATION ph DO ANTIFOAM EXHAUST Working volume 3L 5L 7L 10L 15L 20L 3L 5L 7L 10L Total volume 3.7L 6.8L 8.9L 12.5L 18L 24.4L 3.7L 6.8L 9.1L 13L Design All vessels comprise autoclavable borosilicate glass contruction with 316L stainless steel headplate with fittings Design features (A) Compact control station with integrated controller including built-in pumps and side panel capable of supporting utilities; (B) storage for 59,994 process programmes; (C) log data storage for 10 process monitoring files; (D) real-time trend data recording; (E) industrial standard ABS front panel with painted iron housing Display 10.4-inch (265mm) colour touchscreen interface supplied as standard with each control station Function Fermentation and cell culture monitoring and control Dimensions Footprint (W x D): 400 x 500mm (15.75 x 19.69"); Height: 740mm (29.13") Probe Drive Impellers Baffles Gas flow options Gas mixing Sparger Inlet filter Filter Condenser 5 C above coolant to maximum 90 C (A) Dry-heating system with programmable 15-step PID control; (B) automatic solenoid-controlled cooling water valve Dry-heating blanket and cooling loop (see A and B above) Dry-heating base unit and cooling loop (see A and B above) Platinum RTD probe (Pt100) Removable top 'brushless' motor rpm standard motor for routine fermentation and cell culture; 5-300rpm option for shear sensitive cell lines Manual setting or programmable 15-step PID control 3 pieces of adjustable Rushton-type impeller for less shear-sensitive microbial cell types (2 pieces of adjustable pitched-blade impeller for fragile cell types - optional) Removable 316L stainless steel baffle for fermentation Standard built-in air-flow rotameter (1-10Lpm for 3/5L; 2-20Lpm for 7/10L; 4-50Lpm for 15/20L vessels); optional O2 enrichment module with rotameter and enrichment valve (bespoke TMFC possible - please enquire) Manual / automatic air and O2 mixing; additional 4-gas mixing station available Orifice ring sparger supplied as standard 0.2μM absolute filter 2-14 ph; 0.01pH resolution PID, linked to acid/base pumps; one-point control with adjustable deadband (ph control range) Gel-filled, autoclavable ph probe 0-200%; 0.1% resolution PID, 2-stage cascade: starting with agitation (1), cascading to optional O2 enrichment (2) if agitation speed is maximum but DO is below setpoint; substrate feeding programme (3) activated at DO setpoint Autoclavable, polarographic DO probe On/off 316L stainless steel with insulated Teflon tube 0.2μM absolute filter 316L stainless steel PUMPS 4 built-in, assignable, with manual setting or programmable 15-step PID control; 0-65rpm (RE-485 connection on control station for fifth pump option) UTILITIES Water / gas / power 2 bar maximum (29 PSI) / 1 bar maximum (14.5 PSI); dry and oil-free / V 50/60Hz or V 50/60Hz; electrical safety cut-off switch *Specifications may be subject to change without notice 7
8 DOUBLE-WALL ROUND BOTTOM SYSTEMS ORDERING INFORMATION ler Thermostatic system control station with built-in rotameter & 4x built-in peristaltic pumps Double-wall vessel (including glass body, head plate, T-shaped hand-lifters & 2 probe adaptors); 2 pieces adjustable pitched-blade impeller; & baffle, condenser & sparger assemblies Motor Agitation motor Probes 1x ph probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc filters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION CSLFS-01-A03P-110/220V CSLFS-01-A05P-110/220V double-wall round bottom vessel, 3L double-wall round bottom vessel, 5L CSLFS-01-A07P-110/220V CSLFS-01-A10P-110/220V double-wall round bottom vessel, 7L double-wall round bottom vessel, 10L Mammalian & insect cell culture SINGLE-WALL ROUND BOTTOM VESSEL SYSTEMS ler Thermostatic system control station with built-in rotameter & 4x built-in peristaltic pumps Single-wall vessel (including glass body, head plate, T-shaped hand-lifters & 2 probe adaptors); 3 pieces adjustable Rushton-style impeller; & baffle, condenser & sparger assemblies Motor Agitation motor Probes 1x ph probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc filters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION CSLFS-01-B03P-110/220V CSLFS-01-B05P-110/220V single-wall round bottom vessel, 3L single-wall round bottom vessel, 5L AIR-LIFTER VESSEL SYSTEMS CSLFS-01-A07P-110/220V CSLFS-01-A10P-110/220V Complete thermostatic bioreactor and fermentation system withsingle-wall round bottom vessel, 7L single-wall round bottom vessel, 10L Anaerobic & aerobic microbial fermentation ler Thermostatic system control station with built-in rotameter & 4x built-in peristaltic pumps Air-lifter vessel (including glass body, head plate, draft tube, T-shaped hand-lifters & 2 probe adaptors), condenser & micro sparger assemblies Motor n/a Probes 1x ph probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc filters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION CSLFS-01-C051P-110/220V single-wall air-lifter vessel, 5L CSLFS-01-C052P-110/220V HEAT-BLANKETED SINGLE-WALL ROUND BOTTOM VESSEL SYSTEMS double-wall air-lifter vessel, 5L Culture of plant & shear-sensitive cells ler Dry-heating system control station with built-in rotameter & 4x built-in peristaltic pumps Single-wall round bottom vessel with heating blanket (including glass body, head plate, draft tube, T-shaped hand-lifters & 2 probe adaptors); 3 pieces adjustable Rushton-style impeller; & baffle, condenser & sparger assemblies Motor Agitation motor Probes 1x ph probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc filters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench PARTNUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION CSLFS-03-B03P-110/220V CSLFS-02-B05P-110/220V CSLFS-02-B07P-110/220V single-wall round bottom vessel, 3L single-wall round bottom vessel, 5L single-wall round bottom vessel, 7L CSLFS-02-B10P-110/220V CSLFS-02-B15P-110/220V CSLFS-02-B20P-110/220V SINGLE-WALL PLAIN BOTTOM VESSEL SYSTEMS WITH HEATING BASE single-wall round bottom vessel, 10L single-wall round bottom vessel, 15L single-wall round bottom vessel, 20L Fermentation of anaerobic & aerobic microbes & culture of temperature- and shear-sensitive cells ler Dry-heating system control station with built-in rotameter & 4x built-in peristaltic pumps Single-wall plain bottom vessel (including glass body, head plate, T-shaped hand-lifters & 2 probe adaptors) with heating base; 3 pieces adjustable Rushton-style impeller; & baffle, condenser & sparger assemblies Motor Agitation motor Probes 1x ph probe with cable; 1x DO probe with cable; 1x temperature probe with cable; 1x foam level sensor with cable Start-Up Kit 4x 250mL & 1x 500mL glass feeding bottles; 10x 45mm, 0.2μm autoclavable disc filters; 15x connecting tubes; 20x silicon tubing clamps; 1x 7.5-m-long 3.1mm diameter silicon tube; 1x handy burner, 1x 2mm Hex wrench & 1x 12mm & 14mm double open-end wrench PART NUMBER DESCRIPTION PART NUMBER DESCRIPTION APPLICATION CSLFS-01-D03P-110/220V CSLFS-01-D05P-110/220V single-wall plain bottom vessel, 3L single-wall plain bottom vessel, 5L CSLFS-01-D07P-110/220V CSLFS-01-D10P-110/220V single-wall plain bottom vessel, 7L single-wall plain bottom vessel, 10L Anaerobic, aerobic & micro-aerobic microbial fermentation 8 For technical advice & further information & pricing on optional ORP probes, impeller choices, 5-300rpm agitation motor, external peristaltic pump, O2 enrichment module & TMFC, please contact your distributor or sales@cleaverscientific.com.
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