Nicolet Evolution 300. UV-Visible Spectrophotometer
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1 Nicolet Evolution 300 UV-Visible Spectrophotometer Analyze Detect Measure Control
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3 Nicolet Evolution 300 UV-Vis An Intelligent, High-Performance System The Nicolet Evolution 300 spectrophotometer, from Thermo Electron Corporation, is the next generation UV-Vis offering ease of use and powerful performance like no other system before it. The legacy of the Nicolet Evolution 300 system spans over 60 years of UV-Vis experience. Built on proven technology, the system s entirely new UV-Vis platform outperforms the competition. The Nicolet Evolution 300 UV-Vis delivers solid, verifiable performance effortlessly day after day. Validation qualification is an integral part of this unique system. Mercury pen and UV wavelength calibration capabilities, plus new 21 CFR Part 11 software tools, help meet and exceed most regulatory requirements. Compliance is streamlined and simplified. Intelligent Accessories, instant sample recognition and quick traceability are just a few of the features that make it the best spectrophotometer for ease of use and superior results. Introduce the Nicolet Evolution 300 system to your lab and see how its innate intelligence and ability will improve performance. Double-beam design provides exceptional stability and low drift High-resolution, variable bandwidth for optimal instrument settings Premium-quality optics offer high throughput and sensitivity Intelligent Accessories and automated logging of sample identification for ease of use Local Control and/or PC control to meet multiple laboratory needs Dedicated applications for life science, pharmaceutical and QC laboratories Tools for 21 CFR Part 11 compliance and multiple security options Validation, qualification and calibration to assist you with full regulatory compliance
4 Refined Optical Design for Confidence in your Results The Nicolet Evolution 300 optical system is designed to ensure that every measurement can be made with confidence. The Nicolet Evolution 300 instrument is based on a cast-aluminum optical chassis, with optical components bolted directly to the base to provide superior system stability. Quartz-coated optical surfaces and a sealed optical system resist degradation, even in aggressive environments. Large, 90% reflective mirrors and a single Ebert monochromator maximize the energy throughput. Interferometric gratings give exceptional stray light performance, allowing measurements up to 6 A. Wavelength accuracy and measurement repeatability are guaranteed by a stepping monochromator that drives the grating directly. The micrometer drive stops at each data point, which eliminates the peak distortions and wavelength shifts associated with traditional designs. The instrument achieves outstanding bandwidth reproducibility by using discrete apertures with photo-etched knife edges and geometry optimized for the Ebert monochromator. The entry and exit slits are mounted on a single module, ensuring that wavelength accuracy is maintained at all bandwidths. The performance of the Nicolet Evolution 300 system is evaluated by built-in diagnostics. This means you can quickly and easily confirm that the instrument is always performing to specifications. Calibration Accessory Holder Filter Wheel Xenon Source Bandpass Selection Grating Ebert Style Monochromator Stepping Micrometer Drive Detector Calibration Validation Carousel Calibration Test Unit
5 Foolproof Calibration and Validation Each instrument is factory calibrated using emission lines from the mercury spectrum to assure absolute wavelength accuracy across the entire UV-Vis range. In addition, an intelligent Mercury Lamp accessory is available to end users for system verification and calibration. Like many labs, you may need to prove your UV-Vis spectrophotometer is within specifications for regulatory standards. Our instrument s proprietary intelligent Calibration Validation Carousel (CVC) contains calibrated wavelength and absorbance filters which are secondary Certified Reference Materials (CRMs) traceable to NPL 1 or NIST 2 via our own ISO Standard calibration process. Recalibration is available from Thermo Electron s UKAS 3 and NVLAP 4 certified recalibration laboratory. Alternatively, the intelligent Instrument Calibration Test Unit can be permanently installed within the optical system at a beam focus. It provides automatic unattended instrument calibration checks and can make the instrument traceable to NPL or NIST by calibrating it against the CVC. Thermo Electron can supply and calibrate a variety of other NIST and NPL CRMs and calibration materials for manual testing of all UV-Vis and visible spectrophotometers. Primary NIST Traceable Reference Materials (NTRMs) are also produced and calibrated by Thermo Electron s UV-Vis calibration laboratory. Wavelength Accuracy Absorbance Accuracy Stray Light Measurement Noise Measurement Drift Measurement NPL and NIST Traceability ISO Standard UKAS Accreditation Certified Reference Materials Primary NIST Filters 1 National Physical Laboratory, UK 2 National Institute for Standards and Technology, USA 3 United Kingdom Accreditation Service, UK 4 National Voluntary Laboratory Accreditation Program, USA
6 Your Choice for System Control The Nicolet Evolution 300 UV-Vis can be controlled via an on-board computer or an external PC. Both options are available for laboratories requiring ultimate flexibility. Local Control The Nicolet Evolution 300 Local Control systems are ideal for laboratories that require high performance and built-in methods in a compact, yet simple-to-use instrument. The spectrophotometer features an integrated, VGA-quality color display and rugged, tactile keypad. The system is compatible with a variety of printers and includes an option for a built-in unit. Data and methods can be stored in the instrument s memory or to the standard floppy disk drive. Data can also be exported to a LIMS system or an external PC via the RS232 port. In addition to a wide selection of standard functions, the Local Control version of the Nicolet Evolution 300 system offers several dedicated life science methods. PCControl VISIONpro and VISIONsecurity are the principal PC software platforms available for the Nicolet Evolution 300 system. These software packages comply with Thermo Electron s UV-Vis development cycle, and are consistent with FDA guidelines and IEEE Software Engineering Standards. VISIONpro is the standard software. It offers advanced scanning, single and multiple wavelength measurements, and single and multi-component quantitative analysis. VISIONsecurity has all the features of VISIONpro, plus functions required for achieving 21 CFR Part 11 compliance. The software offers multiple levels of security, access control, audit trails and Electronic Signatures. VISIONlife is an optional module for VISIONpro or VISIONsecurity. VISIONlife adds kinetics, parallel rate, DNA denaturing 1, Advanced Enzyme Kinetics and equation modeling. Additional software packages available for the Nicolet Evolution 300 UV-Vis include: VISION Auto used for automated analysis of multiple samples; VISION Chroma for color measurements; and Dsolve (21 CFR Part 11 compliance package) required in dissolution studies, and dedicated food and wine analysis packages. Life Science Functions 260/280 and 260/230 ratios Multiple cell growth methods Direct reading methods Oligo calculator Modeling of enzyme behavior 2 Advanced Kinetics 2 DNA melting 1 Standard Functions SmartStart (Local Control only) personal set-up Single wavelength Multiple wavelengths Ratio and corrected ratio Advanced scanning Kinetics Parallel rate Quantitation Advanced results calculation Multi-component analysis Multi-level access and security controls 21 CFR Part 11 tools 2 Electronic Signatures 2 Report composer 2 Multiple language support 1 Hardware dependent 2 PC only
7 Intelligent Accessories Complete the Next Generation System Intelligent Accessories, now available for the Nicolet Evolution 300 UV-Vis, offer an unmatched level of automation making sampling easier than ever before. The cell changers and sample holders feature auto-recognition, smart alignment and serial number reporting which saves you time and enhances productivity. Intelligent Accessories allow easy interchange and provide assurance that the correct accessory is appropriately fitted. Simply snap the accessory in place and let the system do the work. Choose from a comprehensive selection for your analysis needs: a b Intelligent Linear Cell Changer (a): fast, precise action for rapid measurement of up to 16 samples, in manual, semi automatic or fully automatic modes. It supports standard cells, microcells, dissolution flow cells and solid samples of varying pathlengths. Intelligent Thermostat-Ready 7-Position Rotary Cell Changer (b): for 7 standard 1 to 10 mm pathlength cells. Intelligent Single Position Peltier Temperature Controlled Cell Holder (c): for temperature studies (e.g. DNA denaturation). Provides temperature control from 5 to 100 C. For single, 1 to 10 mm pathlength cells. Intelligent Radio Frequency Identification (RFID) Unique Cell Identifier: reads the identity of cells fitted with an RFID emitter at the time of measurement. Cell identities are saved and/or printed out with results. Intelligent Sipper (d): multi-roller peristaltic pump mounted within the sample compartment. Sip and Sip-and-run operating modes are supported. Samples may be pumped to waste or returned. Allows for calibration of tubing volume. Minimum sample volume 200 µl with a single, standard cell holder. Requires a flowcell. Intelligent Instrument Calibration Validation Carousel (CVC): automatic testing of UV and visible wavelength accuracy, UV and visible absorbance accuracy, UV and visible stray light, resolution, noise and stability. Absorbance and wavelength filters are traceable to NPL or NIST via an ISO Standard accredited calibration process. The CVC is supplied with a calibration certificate and shipping box for recalibration. Intelligent Instrument Calibration Test Unit (CTU): automatic testing of UV and visible wavelength accuracy, visible absorbance accuracy, UV and visible stray light, instrument noise and instrument stability. Absorbance and wavelength filters can be made traceable to NPL or NIST with use of the CVC. The unit fits under the instrument cover within the optical system at a beam focus and is either factory or field installed. Fiber Optic Sampling Accessory (e): enables samples to be measured outside the sample compartment. Mercury Lamp: provides means for wavelength calibration and verification in the ultraviolet and visible spectral ranges, using six fundamental mercury emission lines. Standard, Baseplate Mounted Accessories: Thermostat-Ready, Single-Position Cell Holder (f): for cells of 1 to 10 mm pathlength. Variable-Pathlength Single Cell Holder (g): for cells of 1 to 50 mm pathlength. Cylindrical Cell Holder (h): for cylindrical cells of 50 mm and 100 mm pathlength. Test Tube and Vial Holder (i): for test tubes from 10 to 24 mm in diameter and 50 to 120 mm high. Temperature Probe Accessory for Intelligent Accessories. Micro Adjustable Cell Holder (j): for micro cells, providing fine adjustment of cell position. Standard Cell Holder (k): (2 supplied with each instrument). c f h j e d g i k
8 Product Specification Nicolet Evolution 300 UV-Vis Spectrophotometer Specifications Optics/Performance Guaranteed (Typical) Wavelength Range 190 to 1100 nm Photometric Range 6.0 A Photometric Display to 6.0 A Detector Photodiode Bandwidth Variable 0.5, 1.0, 1.5, 2.0, 4.0 nm Best Data Resolution 0.1 nm Scan Speeds 1 to 3800 nm/min Monochromator Drive Speed 4000 nm/min Wavelength Accuracy 1 ± 0.3 nm (± 0.1 nm) Wavelength Repeatability 2 ± 0.1 nm (± 0.03 nm) Photometric Accuracy 3 1 A ± A (± A) 2 A ± A (± A) 3 A ± A (± A) European Pharmacopoeia Photometric Accuracy nm ± A 257 nm ± A 313 nm ± A 350 nm ± A Photometric Repeatability 5 1 A ± A 2 A ± A 3 A ± A Peak-to-peak Noise 0 A at 1.5 nm < A (< A) 1 A at 1.5 nm < A (< A) Stray Light KCl solution at 200 nm > 2.0 A (> 2.5 A) NaI solution at 220 nm < 0.02%T (< 0.005%T) NaNO 2 solution at 340 nm < 0.02%T (< 0.005%T) Baseline flatness 6 ± A (± A) Stability at 340 nm > A/hr (± A/hr) Dimensions (w x d x h), mm 610 x 530 x 380 Dimensions (w x d x h), inches 24 x 21 x 15 Weight 22 Kg (48.5 lbs) Electrical Supply V, Hz 1 Wavelength accuracy using holmium and didymium traceable to National Physical Laboratory (NPL), 190 to 1100 nm 2 Wavelength repeatability across complete wavelength range using holmium and didymium traceable to NPL (Typical 10 scans of Mercury line) 3 Photometric accuracy at 546 nm using neutral density glass filters traceable to NPL 4 European Pharmacopoeia Photometric accuracy test with potassium dichromate 5 Photometric repeatability at 546 nm using neutral density glass filters traceable to NPL 6 Baseline flatness 200 to 800 nm USA 5225 Verona Road, Madison, WI Tel: , Fax: spectroscopy@thermo.com UK 8 Mercers Row, Cambridge, CB5 8HY, UK Tel: +44 (0) Fax: +44 (0) spectroscopy.uk@thermo.com Switzerland En Vallaire Ouest C, case postale, CH-1024, Ecublens Tel: +41 (0) Fax: +41 (0) spectroscopy.ch@thermo.com Thermo Electron Corporation has direct subsidiary offices in North America, Europe and Japan. To complement these direct subsidiaries, we maintain a network of representative organizations throughout the world. Use this reference list or visit our Web site to locate the representative nearest you. Australia Tel: +61 (0) sales@thermo.com.au Austria Tel: +43 (0) info.austria@thermo.at Belgium Tel: info@thermo-optek.be Canada Tel: sales@thermoinstruments.ca China Thermo ARL: Tel: info@arl-china.com Thermo Elemental: Tel: thermo@thermo.net.cn Thermo Nicolet: Tel: ext nicolet.bj@nicolet.cc France Tel: +33 (0) blaurent@thermo-optek.fr Germany Tel: +49 (0) info@thermospectroscopy.de Italy Tel: vendite@thermo-optek.it Japan Thermo Electron KK: Tel: info@nicolet.co.jp Netherlands Tel: infooptek@thermoelectron.nl Nordic Thermo Nicolet: Tel: info@thinab.se Thermo ARL: Tel: +46 (0) info@thinab.se South Africa Tel: +27 (0) sales@thermoarlabs.co.za Spain Tel: +34 (91) comercial@thermo.es FM ISO Calibration No Accreditation applies to wavelength and Absorbance filters Thermo Electron Corporation. All rights reserved worldwide. We make no warranties, expressed or implied, in this product summary, and information is subject to change without notice. All product and company names are property of their respective owners. P/N /03 BR-S0390
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