UVP, Inc. Ultra-Violet Products Ltd., Unit W 11th Street, Upland, CA Trinity Hall Farm Estate, Nuffield Road

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1 SI-950 Ultraviolet Incubator User Instructions UVP, Inc. Ultra-Violet Products Ltd., Unit W 11th Street, Upland, CA Trinity Hall Farm Estate, Nuffield Road Tel: (800) / (909) Cambridge CB4 1TG UK Fax: (909) Tel: +44(0) / Fax: +44(0) Web Site: Rev. F

2 Introduction This single chamber unit is designed for the variable-temperature incubation and humidification envelopment of microbiology organisms, biology assays, and any protocol requiring heat incubation in the range of ambient +10 C to 68 C. The Incubator is constructed with built-in overhead shortwave 254nm UV tubes for sterilization of the chamber between experiments. Safety Information The UV Incubator is designed with function, reliability, and safety in mind. It is the user s responsibility to install it in conformance with local electrical codes. Specifications Temperature Range Ambient to 68 O C Sensor LM345 Integrated Temperature Sensor Controller PI Setpoint Digital Display Digital LED Accuracy +1 O C Uniformity +0.5 O C at 37 O C Relative Humidity ~ 80% Unit Heat Load Typical operation Heating Element Dimensions Exterior W x H x D Interior W x H x D Incubation 1250 Watts, 4,265,1777 BTUXH 17.5 x 17 x 14 (445 x 432 x 356mm) 14 x 10.7 x 10.9 (356 x 272 x 277mm) Shelves (2 standard) Dimensions (W x D) 13.3 x 8.5 (338 x 216mm) Construction Formed stainless steel Surface area in 2 Construction Interior volume cu ft Interior Exterior Door 0.95 cubic feet (26.9 liters) Stainless steel Aluminum powder coated Acrylic Electrical 115V 230V 100V 60 Hz 50 Hz 50/60 Hz

3 UV Incubator Page 2 Additional Specifications Pollution degree 2% Installation Category II Altitude 2000m Indoor Use Incubator is designed for operation inside a building only Main supply Not to exceed 10% of the nominal supply voltage. Setting-Up the Incubator 1. Remove the incubator from the packaging. 2. Place the unit on a level working surface and provide adequate room in front of the door to open it easily. 3. Plug the female end of the power cord into the back of the unit. 4. Plug the male end of power cord into an electrical outlet. 5. Press the O/I toggle switch located in front of unit to turn the incubator on. The touch pad should illuminate. 6. Select the desired temperature by pressing the touchpad up and down arrows. 7. The internal temperature will be displayed on the touchpad. Operating the Incubator Setting the Temperature When the power is turned on, the LED display flashes the software version number for several seconds. The display will then show the internal ambient temperature for several seconds, and adjust to the set temperature (displayed in degrees Celcius). This setting is adjustable from ambient temperature to 68 o C. The current setpoint value can be altered by pressing the UP and DOWN buttons on the touchpad while the value is being displayed on the LED display. To change the setpoint: Momentarily press then release either the UP or the DOWN button. The LED intensity will flash to indicate that the displayed value is the current setpoint. Increase or decrease the setpoint value by pressing UP or DOWN buttons respectively. If either button is held down for more than several seconds, setpoint value will increase or decrease continuously. When the desired setpoint is reached, wait approximately five seconds without pressing either button; the display will revert to normal mode showing the actual temperature.

4 UV Incubator Page 3 The new setpoint becomes effective and is stored in the non-volatile memory when the display reverts to the normal mode. Calibrating the Temperature Reading The incubator is calibrated at the UVP factory at 37 o C. UVP recommends temperature recalibration be performed at the UVP factory as recalibration by the user may void the warranty. Obtain a RGA (Returned Goods Authorization) number from UVP prior to returning a unit to the factory. Call UVP s customer service department for information. Setting the Sterilization Cycle The SI-950 UV Incubator provides an automated process for eliminating contamination by utilizing the germicidal properties of ultraviolet light. The UV light is typically operated when no samples are inside the chamber. The entire chamber is sterilized eliminating viable fungi, bacteria, and yeast. Visible light sterilization makes the SI-950 ideal for cgmp sterility testing and BL3 laboratories. To operate the sterilization function, turn the master power on, and then turn the UV keylock to operate the germicidal lamp housed within the chamber. Insert the UV key to operate the shortwave UV. Remove the key after using the UV to avoid accidental exposure of samples during incubation. UV key turned in the OFF position. UV key turned in the ON position. [Note] Do not attempt to incubate cultures with the germicidal lamp on. The germicidal lamp is used to sterilize the internal chamber of the incubator in between experiments. [Warning] Exposure to UV light is dangerous. Keep the door closed. The incubator door is UV blocking and will not allow UV radiation to pass through the door. The germicidal lamp will shut off if the door is opened. A Word of Caution: The UV Incubator contains a powerful source of UV radiation that will cause damage to unprotected eyes and skin. Before operating any unit, be sure all personnel in the area are properly protected. It is preferable that the unit be installed and operated in a area where access and exposure is limited while the

5 UV Incubator Page 4 unit is in operation. Even though the unit shuts the UV off when the door is open, UV Blocking Eyewear should be worn as well. UVP has a complete line of UV Blocking Eyewear: Spectacles, Goggles, and Faceshield designed for this purpose. Extended periods of time can be used to disinfect the interior incubator chamber by the destruction of organisms such as: Bacillus anthracis Bacillus subtilus Corynebacterium Escherichlia Coli Micrococcus candidus Mycobacterium tuberulosis Proteus vulgaris Salmonella Streptococcus hemolyticus Aspergillus niger Saccharoyces ellipoideus Influenza Chlorella vulgaris And others Recommendations for UV Sterilization Typically the germicidal lamp is used during periods of non-use (overnight and during weekend periods of none cultivation) to insure a clean non-infectious incubatory surface exists prior to critical sample testing. Care and Cleaning The units are built to provide trouble-free operation. NOTE: The door is made of a polycarbonate plastic material and is subject to scuffing and scratches if improperly cleaned. To ensure correct operation: Wipe excess water from inside the unit and outside the unit with an absorbent soft cloth or sponge. Use mild soap and water with a soft cloth or sponge to clean the exterior of the unit. Use a mild detergent on the interior surfaces. Warning: Unplug the UV incubator before cleaning around the UV bulb contacts. Clean the door with a mild detergent; never use organic based compounds, Alcohol, or Ammonia containing cleaners. Do not use abrasive pads or cleansers. A plastic cleaner solution is recommended to clean the door and is available from local plastic supply distributors.

6 UV Incubator Page 5 Replacement Parts and Replacement Instructions Replacement parts are listed below for the SI-950 UV Incubator. Authorized service personnel shall do repairs or replacements other than specified in the following procedures. Key, replacement Tube, 8-watt, 254nm shortwave germicidal Tray, stainless steel ventilated Spectacles, UV blocking (UVC-303) Goggles, UV blocking (UVC-503) Face Shield, UV blocking (UVC-803) Replacing the UV Germicidal Bulb Turn the unit power off and unplug the unit from the outlet. Open the incubator door and locate the bulb. The bulb has two prongs on each end that first fit into a socket than rotate into place. Using two hands, grab the bulb and fit the bulb end into the socket. Twist the bulb 90 until the bulb locks into place. Maintenance, Repair and Technical Assistance UVP offers technical support for all of its products. If you have any questions about the product s use, operation or repair, please contact our offices at the locations below. Note: A Returned Goods Authorization (RGA) number must be obtained from UVP Customer Service before returning any product. You may contact UVP s Customer Service based on your location: If you are in North America, South America, East Asia or Australia: Call (800) or (909) , and ask for Customer Service during regular business days, between 7:00 a.m. and 5:00 p.m. PST. your message to: info@uvp.com Fax Customer Service, and send it to: If you are in Europe, Africa, the Middle East or Western Asia: Call +44(0) , and ask for Customer Service during regular business days, between 8:00 a.m. and 5:30 p.m. your message to: uvp@uvp.co.uk Fax Customer Service, and send it to:

7 UV Incubator Page 6 (909) (0) Write to: UVP, Inc W. 11 th Street, Upland, Write to: Ultra-Violet Products Ltd., Unit 1, Trinity CA USA Hall, Nuffield Road, Cambridge CB4 1TG UK Warranty UVP, Inc. warrants its products to be free of detects in materials and workmanship for a period of one (1) year from date of purchase. The foregoing warranty of UVP shall be of no force and effect if buyer has modified or damaged the product. Tubes and filters are warranted for 90 days. All warranties or merchantability and fitness for any purpose and all other warranties, expressed or implied, except those expressly set forth herein, are deemed waived and excluded. UVP s duty under the warranty is limited to replacement and/or repair of the defective part at the option of UVP, Inc. UVP shall not be liable for any expenses or damages incurred by the purchaser except as expressly set forth herein, and in no event shall UVP be liable for any special, incidental or consequential damages of any kind. This warranty does not supersede any statutory rights that may be available in certain countries. UVP Providing Quality Products for the Researcher Since 1932 From its start in 1932, UVP has become a story of growth and value fulfillment. Today, UVP services the needs of science, industry and education throughout the world. A special significance of UVP research and development arises from our constant communication with customers. UVP continually invites your comments toward the improvement of UVP products or the development of custom types of ultraviolet light sources. UVP will work with you through development and production of a product that meets your specific needs. From all of us at UVP, Thank You. UVP, Inc West 11 th Street Ultra-Violet Products Ltd Upland, California Unit 1, Trinity Hall Farm Estate, Nuffield Road Tel: (800) (909) Cambridge CB4 1TG UK Tel: +44 (0) Fax: (909) Fax: +44 (0) Web site: