Estrus Synchronization for Beef Cattle
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1 Estrus Synchronization for Beef Cattle Cliff Lamb State Beef Specialist University of Minnesota
2 Estrous Synchronization Protocols MGA/F Hybrid Synch 7-11 Synch Two shot F Select Synch MGA Select Hybrid Synch+CIDR Presynch Resynch One shot F CO-synch Ov-synch CIDR/F CO-synch+CIDR Heat Synch
3 HEAT DETECTION Select Synch BEEF COWPROTOCOLS HEAT DETECT & TIME AI (TAI) Select Synch & TAI Heat detect and AI day 4 to 10 and TAI all non-responders hr after with at TAI. 0 4 Select Synch + CIDR Select Synch + CIDR & TAI Heat detect and AI day 7 to 10 and TAI all non-responders hr after with at TAI hr.. AI 10 0 CIDR CIDR hr.. AI 10 MGA Select MGA Select & TAI Heat detect and AI day 33 to 36 and TAI all non-responders hr after with at TAI. MGA d 7 d AI MGA 12 d 7 d hr FIXED-TIME AI (TAI) CO-Synch + CIDR Perform TAI at 66 hr after with at TAI. MGA Select AI CIDR.. 66 hr Perform TAI at 72 hr after with at TAI. AI MGA 12 d 7 d.. 72 hr COMPARISON OF PROTOCOLS FOR BEEF COWS HEAT DETECTION COST LABOR Select Synch Low /High Select Synch + CIDR High MGA Select /High HEAT DETECT & TAI Select Synch (TAI non-responders hr after ) Low /High Select Synch + CIDR (TAI non-responders hr after ) High MGA Select (TAI non-responders hr after ) /High FIXED-TIME AI (TAI) CO-Synch + CIDR (TAI at 66 hr after with at TAI) High MGA Select (TAI at 72 hr after with at TAI) High The times listed for Fixed-time AI should be considered as the approximate average time of insemination. This should be based on the number of cows to inseminate, labor, and facilities. Cystorelin, Factrel, Fertagyl, OvaCyst D.J. Schafer and D.J. Patterson Division of Animal Sciences - University of Missouri Columbia These protocols are recommended by the North Central Region Bovine Reproduction Task Force. Estrumate, In-Synch, Lutalyse, ProstaMate
4 HEAT DETECTION 1 Shot BEEF HEIFER PROTOCOLS HEAT DETECT & TIME AI (TAI) CIDR CIDR Select Synch + CIDR & TAI Heat detect and AI day 7 to 10 and TAI all non-responders hr after with at TAI. CIDR hr.. AI MGA - & TAI Heat detect and AI day 33 to 36 and TAI all non-responders hrs after with at TAI. MGA - MGA 19 d MGA d AI hr FIXED-TIME AI (TAI) CO-Synch + CIDR Perform TAI at 54 hr after with at TAI. AI CIDR.. 54 hr MGA - Perform TAI at 72 hr after with at TAI. AI MGA 19 d.. 72 hr COMPARISON OF PROTOCOLS FOR BEEF HEIFERS HEAT DETECTION COST 1 Shot Low CIDR - MGA - Low HEAT DETECT & TAI Select Synch + CIDR High (TAI non-responders hr after ) MGA - (TAI non-responders hr after ) FIXED-TIME AI (TAI) CO-Synch + CIDR High (TAI at 54 hr after with at TAI) MGA - (TAI at 72 hr after with at TAI) LABOR High Low/ The times listed for Fixed-time AI should be considered as the approximate average time of insemination. This should be based on the number of heifers to inseminate, labor, and facilities. Cystorelin, Factrel, Fertagyl, OvaCyst D.J. Schafer and D.J. Patterson Division of Animal Sciences - University of Missouri Columbia These protocols are recommended by the North Central Region Bovine Reproduction Task Force. Estrumate, In-Synch, Lutalyse, ProstaMate
5 THE ESTROUS CYCLE CL Follicles E2 P4 Estrus Estrus
6 Synchronization Products Prostaglandin (F) Lutalyse Prostamate In-Synch Estrumate
7 X Corpus Luteum X Progesterone Ovulation Follicle
8 Synchronization Products Progestins Melengestrol Acetate (MGA) CIDR
9 Corpus Luteum Follicle X Progesterone Ovulation
10 Concentrations of Progesterone in Ovariectomised Cows Produced by CIDR Containing 1.9g and 1.38g Progesterone Over a 20 Day Treatment 6 Plasma progesterone concentration (ng/ml) Time (days) ( = CIDR 1900 Cattle insert, = CIDR 1380 Cattle insert, N = 10, error bars = SEM)
11 Synchronization Products Gonadotropin Releasing Hormone () Cystorelin Factrel Fertagyl Ova-Cyst
12 Corpus Luteum Progesterone Ovulation Follicle
13 Definitions Synchronization Rate: % of females detected in estrus compared to total number synchronized. Conception Rate: % of females pregnant compared to number of females inseminated. Pregnancy Rate: % of females pregnant compared to total number synchronized.
14 CIDR Based Protocols
15 CIDR Protocols in Heifers
16 CIDR- & TAI CIDR F Detect estrus & AI TAI & Select Synch+ CIDR & TAI CIDR F Detect estrus & AI TAI & CO-Synch+CIDR CIDR F TAI & CIDR-+TAI CIDR F TAI & -18 to B B Days relative to F Hours relative to F
17 First Service Pregnancy Rates % n = 282/517 n = 289/504 n = 282/531 n = 258/525 0 CIDR- & TAI Select Synch+CIDR &TAI CO-Synch+CIDR CIDR-+TAI Treatments
18 Pregnancy Rates after Estrus Detection Alone vs TAI % CIDR- & TAI Select Synch+CIDR &TAI CO-Synch+CIDR CIDR-+TAI P = Treatment
19 CIDR- & TAI CIDR F Detect estrus & AI TAI & Select Synch+ CIDR & TAI CIDR F Detect estrus & AI TAI & Days relative to F Hours relative to F
20 Distribution of estrus as a percentage of all heifers treated CIDR- and TAI Select Synch + CIDR and TAI % >84 No estrus Hours after F
21 CIDR Protocols in Cows
22 CIDR- & TAI CIDR F Detect estrus & AI TAI & F TAI & CO-Synch CO-Synch+CIDR CIDR F TAI & F TAI & Select Synch & TAI Detect estrus & AI F TAI & CIDR Select Synch+CIDR & TAI Detect estrus & AI -18 to B B Days relative to F Hours relative to F
23 Effect of Location on Pregnancy Rates % P < 0.01 IL-1 IL-2 IN KS-1 KS-2 MN-1 MN-2 MN-3 MT OH-1 OH-2 OH-3 OH-4 TN Locations
24 First Service Pregnancy Rates % a 52.3 b 43.4 ac ac c n = 266/511 n = 234/551 n = 295/547 n = 269/513 n = 290/ P < 0.05 CIDR- & TAI CO-Synch COSynch+CIDR Select Synch & TAI Treatments Select Sync+CIDR and TAI
25 Pregnancy Rates after Estrus Detection Alone vs TAI 70 % a 43.4 b c a,b b P < 0.05 CIDR- & TAI CO-Synch COSynch+CIDR Select Synch & TAI Treatment Select Sync+CIDR and TAI
26 Characteristics of Estrus CIDR- & TAI CIDR F Detect estrus & AI TAI & F TAI & Select Synch & TAI Detect estrus & AI Select Synch+CIDR & TAI CIDR F Detect estrus & AI TAI & Days relative to F Hours relative to F
27 Distribution of estrus 40 CIDR- & TAI Select Synch & TAI Select Sync+CIDR and TAI % >84 No estrus Hours after F
28 MGA Based Protocols
29 MGA - Perform TAI at 72 hr after with at TAI MGA 0 14d 14 19d 33 Heat detect And AI Treatment day Labor costs: Drug costs: Recommend: Replacement Heifers
30 MGA - Perform TAI at 72 hr after with at TAI TAI & MGA 0 14d 14 19d 33 72hr 36 Treatment day Labor costs: Drug costs: Recommend: Replacement Heifers
31 Select Synch vs. 2 F 2α vs.mga/f 2α F Heat detect and AI F F Heat detect and AI F Heat detect and AI MGA
32 Effect of days postpartum on pregnancy rates after treatment with Pregnancy rates, % 60 Select Synch MGA/F 50 2 F < >144 Time relative to F, h (Lamb et al., 2004)
33 Select Synch vs. 2 vs.mga/ in replacement beef heifers Item 2xF 2a MGA/F 2a Select Synch No. of heifers Synchronization rate, % Conception rates, % Pregnancy rates, % (Lamb et al., 2004)
34 MGA Select Perform TAI at 72 hr after with at TAI TAI & MGA 0 14d 14 12d 26 7d 33 72hr 36 Treatment day Labor costs: High Drug costs: Recommend: Postpartum cows
35 7 11 Synch MGA F F Heat detect and AI Days from F Hours from F
36 MGA/ vs. MGA Select F Heat detect and AI MGA F Heat detect and AI MGA
37 Estrus distribution among cows treated with MGA/F 2α or MGA Select % of cows exhibited estrus 40 MGA Select 35 MGA/F No Hours to estrus Response (Patterson et al., 2003)
38 MGA/F 2α vs. MGA Synch Item MGA/F 2α MGA Select No. of Cows Synchronization rate, % 76/100 (76) 88/101 (87) Conception rates, % 58/76 (76) 67/88 (76) Pregnancy rates, % 58/100 (58) 67/101 (66) (Patterson et al., 2003)
39 Comparison of estrus response and fertility in postpartum beef cows with various estrous synchronization protocols Treatment SR PR 2 shot 214/422 57% 147/422 35% Select Synch 353/528 67% 237/528 45% MGA- 161/206 78% 130/206 63% 7-11 Synch 142/155 92% 101/155 65% MGA Select Fixed-time AI 281/436 64% (Patterson et al., 2004)
40 HEAT DETECTION Select Synch BEEF COWPROTOCOLS HEAT DETECT & TIME AI (TAI) Select Synch & TAI Heat detect and AI day 4 to 10 and TAI all non-responders hr after with at TAI. 0 4 Select Synch + CIDR Select Synch + CIDR & TAI Heat detect and AI day 7 to 10 and TAI all non-responders hr after with at TAI hr.. AI 10 0 CIDR CIDR hr.. AI 10 MGA Select MGA Select & TAI Heat detect and AI day 33 to 36 and TAI all non-responders hr after with at TAI. MGA d 7 d AI MGA 12 d 7 d hr FIXED-TIME AI (TAI) CO-Synch + CIDR Perform TAI at 66 hr after with at TAI. MGA Select AI CIDR.. 66 hr Perform TAI at 72 hr after with at TAI. AI MGA 12 d 7 d.. 72 hr COMPARISON OF PROTOCOLS FOR BEEF COWS HEAT DETECTION COST LABOR Select Synch Low /High Select Synch + CIDR High MGA Select /High HEAT DETECT & TAI Select Synch (TAI non-responders hr after ) Low /High Select Synch + CIDR (TAI non-responders hr after ) High MGA Select (TAI non-responders hr after ) /High FIXED-TIME AI (TAI) CO-Synch + CIDR (TAI at 66 hr after with at TAI) High MGA Select (TAI at 72 hr after with at TAI) High The times listed for Fixed-time AI should be considered as the approximate average time of insemination. This should be based on the number of cows to inseminate, labor, and facilities. Cystorelin, Factrel, Fertagyl, OvaCyst D.J. Schafer and D.J. Patterson Division of Animal Sciences - University of Missouri Columbia These protocols are recommended by the North Central Region Bovine Reproduction Task Force. Estrumate, In-Synch, Lutalyse, ProstaMate
41 HEAT DETECTION 1 Shot BEEF HEIFER PROTOCOLS HEAT DETECT & TIME AI (TAI) CIDR CIDR Select Synch + CIDR & TAI Heat detect and AI day 7 to 10 and TAI all non-responders hr after with at TAI. CIDR hr.. AI MGA - & TAI Heat detect and AI day 33 to 36 and TAI all non-responders hrs after with at TAI. MGA - MGA 19 d MGA d AI hr FIXED-TIME AI (TAI) CO-Synch + CIDR Perform TAI at 54 hr after with at TAI. AI CIDR.. 54 hr MGA - Perform TAI at 72 hr after with at TAI. AI MGA 19 d.. 72 hr COMPARISON OF PROTOCOLS FOR BEEF HEIFERS HEAT DETECTION COST 1 Shot Low CIDR - MGA - Low HEAT DETECT & TAI Select Synch + CIDR High (TAI non-responders hr after ) MGA - (TAI non-responders hr after ) FIXED-TIME AI (TAI) CO-Synch + CIDR High (TAI at 54 hr after with at TAI) MGA - (TAI at 72 hr after with at TAI) LABOR High Low/ The times listed for Fixed-time AI should be considered as the approximate average time of insemination. This should be based on the number of heifers to inseminate, labor, and facilities. Cystorelin, Factrel, Fertagyl, OvaCyst D.J. Schafer and D.J. Patterson Division of Animal Sciences - University of Missouri Columbia These protocols are recommended by the North Central Region Bovine Reproduction Task Force. Estrumate, In-Synch, Lutalyse, ProstaMate
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