Model 860 Autosampler

Transcription

1 Model 860 Autosampler Operating Manual Copyright 2012 Sherwood Scientific Ltd Issue th March 2012 ECN 735 G:\DOCUMENT CONTROL\Manuals\Autosampler\860\ M860 Operator Manual Issue 10.doc

2 Table of Contents 1.0 Introduction Contacting Sherwood Scientific Safety Precautions Caution and Warning Statements Impaired Safety Protection Warranty Installation Working Environment Unpacking Installation And Connection Layout of Autosampler System Fitting the Autosampler to the Model 805 Diluter (if required) Connecting the Sample Tubing from the Autosampler to a Flame Photometer Connecting the Sample Tubing from the Autosampler to the Diluter Connecting the Communication Cable Connecting the Power Supply Fitting the Safety Cover Instrument Principles of Operation Principles of Operation Use with a Diluter (normally used for clinical samples) Use without a Diluter Use with M410 and BlueNotes for M Technical Specification Sampling Tray Vial Volumes Sample Volumes Sampling Speed Power Requirements Size (with Diluter) Weight Instrument Description Familiarisation Rear of main module: Connectors and DIP switches Top View : Run / Stop keys Front View : Status Leds Front View: Autosampler Probe Operating Instructions Setting the Sampling Method Loading the Sample Tray Switching on the Instrument Blanking and Calibration of the Flame Photometer Blanking with the Diluter (dipswitch 7 Off upper position) Blanking without the Diluter (dipswitch 7 On lower position) Calibration with the Diluter (dipswitch 7 Off upper position) Calibration without the Diluter (diswitch 7 On lower position) Running the samples Using a Diluter (dipswitch 7 Off upper position) Without a Diluter (dipswitch 7 On lower position) Aborting a Sample Run Fault Conditions Issue th March 2012 ECN 735 2

3 Table of Contents 7.0 Replacement Procedures Autosampler Probe Replacement Appendix A 1:50 Dilution Operating the Model 860 with tube Appendix B Updating Firmware Product Warranty Statement Issue th March 2012 ECN 735 3

4 1.0 Introduction 1.1 Contacting Sherwood Scientific Postal address for letters and orders: Sherwood Scientific Ltd 1 The Paddocks Cherry Hinton Road Cambridge CB1 8DH UK Telephone Fax info@sherwoodscientific.com For Certificates of Analysis and Material Safety Data Sheet downloads, contacting Distributors and Technical and Application information on our products go to our Website The following section must be carefully read before unpacking, installing, using, maintaining or repairing the instrument and/or its accessories. 1.2 Safety Precautions The safety statements in this manual comply with the requirements of the UK Health and Safety at Work Act of You must read this section to ensure the safe operation of your instrument The standard safety procedures in your laboratory should always be adhered to, in addition to the advice given in this manual Removal of any fixed covers may expose live electrical mains. If this becomes necessary, the instrument must be disconnected from the electrical mains and at least three minutes allowed, for capacitors to discharge Ensure that the unit is never operated with the safety cover removed. Do not attempt to insert or remove vials whilst the sample tray is moving or whilst a method is running Issue th March 2012 ECN 735 4

5 1.0 Introduction 1.3 Caution and Warning Statements CAUTION: Is used to indicate correct operating or maintenance procedures that prevent damage to or destruction of the equipment or other property WARNING: Is used to indicate a potential danger that requires correct procedures or practices to prevent personal injury. 1.4 Impaired Safety Protection Whenever it is likely that safety protection has been impaired, the instrument and/or accessory must be made inoperative and secured against operation. The matter should then be referred to the appropriate servicing authority for remedial action. 1.5 Warranty Warranty is subject to proper use in accordance with the instructions contained within this manual. The following items however, are specifically excluded from any warranty: Autosample Probe Sample Vials Issue th March 2012 ECN 735 5

6 2.0 Installation Section 1.2 SAFETY, must be carefully read before unpacking the instrument and/or its accessories. Before unpacking any further, the pack should be carefully checked for damage. If any damage is evident, it should be immediately reported to the Supplier and no further unpacking undertaken without their permission. 2.1 Working Environment The M860 Autosampler is designed to be used with the Sherwood Model 420 and Model 425 Flame Photometers and can be used in conjunction with the Model 805 Diluter. If the Model 860 is to be used with the Model 805 Diluter, ensure that the bench upon which the Model 805 Diluter is to stand is level, has a smooth surface, which does not impede underside ventilation and is strong enough to carry the weight of the instrument. The bench area should also be sufficient to accommodate any ancillary equipment in addition to the main instrument and allow for working space. Ensure that the power supply has a protective ground (earth) connection. WARNING: Switch off the Flame Photometer and Diluter then disconnect from the power supply before proceeding with the installation. 2.2 Unpacking The presence of all of the following items should be confirmed. If any items are missing, this must be reported to the Supplier immediately. CAT. NO. ITEM QUANTITY Autosampler, M Operator Manual, M860 Autosampler Universal Power Supply Mains a.c. Supply Lead Cup, Sample, 2ml Cup, Sample, 14ml Tube, Manifold, Internal Standard, pack of Tube, Manifold, Red, Long, pack of Carousel, 40 position Adapter plate Cable, M860 to M420, for flame upto s/n Cable, M860 to M425/420, for flame s/n16446 on Cable, Null Modem Probe Kit, Autosampler CDROM, Flame Training Guide Issue th March 2012 ECN 735 6

7 2.0 Installation 2.3 Installation and Connection The M860 Autosampler is designed to be installable by the end user. However, should suitably trained personnel not be available, then please contact your Supplier who will be able to install the unit for you Layout of Autosampler System The main components of the Model 860 system are shown in the diagram below. The positions of the Diluter and adapter plate, which is necessary if the Diluter is to be used, are also shown. Autosampler Tower Safety cover Autosampler Probe Sample Carousel Autosampler Base (Adapter Plate) Locating Lug (Diluter) Issue th March 2012 ECN 735 7

8 2.0 Installation Fitting the Autosampler to the Model 805 Diluter (if required) Ensure that the unit is powered off and that the Diluter is positioned on a level surface. Locate the fixing lug of the Adapter Plate into the hole at the top left corner at the back of the Diluter, see below. Rotate and place the adapter plate on top of the diluter so that its front edge clips over the front edge of the Diluter Issue th March 2012 ECN 735 8

9 2.0 Installation Lift the Autosampler and locate the feet on its base into the holes in the adapter plate Connecting the Sample Tubing from the Autosampler to the Flame Photometer The Sampling Tube (part number ) is connected to the Nebuliser of the Flame Photometer with the other end is connected directly through the plastic cover to the Autosampler Probe. The tube is held in the slot on the right hand edge of the Autosampler to prevent entanglement Issue th March 2012 ECN 735 9

10 2.0 Installation Connecting the sample tubing from the Autosampler to the Diluter 1:200 Dilution The short end of the Sampling Tube (part number ) is connected to the Manifold of the Diluter as described in the Diluter manual. The other end is connected directly through the plastic cover to the Autosampler Probe. The tube is held in the slot on the right hand edge of the Autosampler to prevent entanglement. 1:50 Dilution Refer to Appendix A Connecting the Communication Cable M420 upto serial number Connect cable to the 6 pin Mini Din socket marked I/O on the back panel of the Autosampler nearest the DIP switches and the other end to the lower 6 pin Mini DIN socket on the rear of the M420. This cable is black and is NOT reversible: the end with the coloured sleeving has to be connected to the Autosampler Socket on the rear of the M420. M425 and M420 from serial number Connect cable to the 6 pin Mini DIN socket marked I/O on the back panel nearest the DIP switches of the Autosampler and the other end to the 6 pin Mini DIN socket marked Autosampler on the back of the Model 420/ Issue th March 2012 ECN

11 2.0 Installation Connecting the Power Supply Please ensure that you have the autosampler connected correctly onto the Model 420/425 before connecting it to the power supply. The electrical connection to the external power supply is made at rear of the instrument, see Figure The universal external power supply will operate from an input supply in the range 90V to 240V a.c., 50Hz or 60Hz. Please ensure that the mains supply you are using is suitable. Fit a suitable 3pin plug to the a.c. supply lead. Check that the correct colour coded leads are connected to the plug terminals. Follow the plug manufacturer s fitting instructions. CAUTION Ensure that the green/yellow earth connector in the a.c. supply lead is connected to a properly grounded earth point, the brown lead is connected to the Line terminal (L) and blue lead is connected to the Neutral terminal (N). The mains power cable ( ), constructed to British Standard 1363, should be connected to the IEC socket on the External Power Supply. A good ground (earth) connection is essential for satisfactory operation of the power supply and the safety of the user. The mains plug must only be inserted into a socket outlet provided with an earth contact. This protective action must not be negated by the use of an extension cable without a protective conductor. WARNING: Any interruption of the protective conductor inside or outside the power supply is likely to make the unit dangerous Fitting the Safety Cover Align the slot in the front of the Safety Cover over the Autosampler Probe and at the same time locate the Metal Pin on the right hand bottom end of the Safety Cover into the slot in the front of the instrument housing. Push the top of the Safety Cover onto the rounded top cover. This is a snug push fit. Note: The right hand top edge should be flush with the housing to ensure that the magnetic sensor functions correctly Issue th March 2012 ECN

12 3.0 Instrument Principles of Operation Model 860 with Model 425 and Model 805 Diluter 3.1 Principles of Operation The Model 860 Autosampler has been designed to work primarily with the Sherwood Scientific Model 420 and Model 425 Flame Photometers, but can also be used with the M410 Flame Photometer when using the BlueNotes for 410 Software. It can be used in conjunction with the Model 805 Continuous Flow Diluter. For the Blank and Calibration sequences, the Model 860 responds to signals from the M420/M425 Flame Photometer and moves automatically to the appropriate vial. Once the Measure button has been pressed, the Model 860 automatically moves from sample to sample sending the signal to the Flame Photometer as indicated below. 3.2 Use with Diluter (normally used for clinical samples) The Model 860 remembers the position of each Blank, Calibration and Measurement sample and sends a signal to the M420/M425 Flame Photometer as that sample enters the Diluter. The Diluter, via the sampling tubing draws the sample through the Autosampler Probe, mixes the sample with the diluent and fills the Weir Cup on the Flame Photometer from which the Flame Photometer aspirates the sample and then measures the sample and displays the result Issue th March 2012 ECN

13 3.0 Instrument Principles of Operation 3.3 Use without a Diluter When used without a Diluter, the Model 860 positions the Autosampler Probe into each Blank, Calibration and Measurement Sample and the M420/M425 Flame Photometer itself draws the liquid into the Nebuliser for aspiration into the flame. The Model 860 sends a signal to the Flame Photometer immediately the Autosampler Probe descends into the sample to initiate the measurement. 3.4 Use with M410 and BlueNotes for 410 Software BlueNotes Refer to the operator manual of the BlueNotes for M410 for details of connection, setup and use with an M410 Flame Photometer Issue th March 2012 ECN

14 4.0 Technical Specification 4.1 Sampling Tray The Autosampler carousel will handle 40 samples plus Blank and Calibration Standard vial. 4.2 Vial Volumes Sample Vials: 2ml Blank and Standard Vial: 14ml 4.3 Sample Volumes Without Diluter Minimum 1.5ml With Diluter Minimum 200µl 4.4 Sampling Speed 30 seconds per sample ±6 seconds in 2 second steps. (set via DIP the switches: see figure 5.1.1) 4.5 Power Requirements External power supply Mains: 90V ±10%, 50/60Hz to 230V ±10%, 50/60Hz. Output: 32V, 1.56A, 50W. 4.6 Size (with Diluter) Height 380mm 535mm mounted on Diluter Width 235mm Depth 210mm 4.7 Weight Net weight Packed weight Packed Dimensions 4.40 Kgs 6.8 Kgs 530 x 360 x 320 mm Issue th March 2012 ECN

15 5.0 Instrument Description 5.1 Familiarisation Rear of main module: Connectors and DIP switches Power socket Power On/Off switch Not Used Not Used I/O for Model 420/425 Method DIP switches Figure 5.1.1(a) Rear view of connectors and dip switches Top View: Run / Stop keys Run Method Key Stop Method Clear Fault Key Figure Run / Stop control keys Issue th March 2012 ECN

16 5.0 Instrument Description Front View: Status LEDs Indicates Fault when flashing Indicates Ready or waiting for Flame Photometer when flashing Indicates Sampling Figure Status LEDs Front View: Autosampler Probe Autosampler Probe Plastic Safety Cover Figure Autosampler Probe and Safety Cover Issue th March 2012 ECN

17 6.0 Operating Instructions Section 1.2 SAFETY, must be carefully read before operating the instrument and/or its accessories. 6.1 Setting the Sampling Method The sampling method is determined by the Model 420/425, the M860 takes signals in sequence during the Blanking and Calibration phase of the M420/M425 startup. Once the M420/M425 is in the Run mode, the Model 860 controls the sequence by a time delay. The Basic time delay is 30 seconds, this delay can be modified by the setting of the row of eight DIP switches located at the rear of the unit, (see figure 5.1.1). The DIP switches are read by the software immediately before each sample. However, it is recommended that they are setup before the unit is powered on and not changed in between samples. Set them up as follows: Method Parameter DIP S/W No. Description Initial Position: All Switches OFF All Switches in Upper position Sampling Delay Time = 30 seconds 1,2,3 For each switch in the ON (lower) position: add a 2 second time increment. 4,5,6 For each switch in the ON (lower) position: subtract a 2 second time increment. 7 Switch to ON (lower) position for use without a Diluter. Autorun 8 Automatically run each sample. i.e. do not wait for READY signal. (Note: except first sample). Include a 10 second delay between samples Issue th March 2012 ECN

18 6.0 Operating Instructions 6.2 Loading the Sample Tray. The first sample vial position is on the right of the sample tray cutout, and vial positions are numbered anticlockwise looking from the top (figure 4.2.1). The sample tray holds up to 40 sample vials plus one Blank Vial and one Standard Vial S B Standard Blank Figure 6.2 Sample Tray (diagrammatic) Fill the Blank Vial with the (Distilled Water) Blank solution and the Standard Vial with the Calibration Standard solution. (If you are not using a Diluter and want to use the Flame Photometer in Internal Reference mode, remember that the Blank, the Standard and the Samples need to include the correct/same level of Lithium Internal Standard). Load the samples anticlockwise from the bottom in adjacent locations and with no empty vial positions in between. If fewer than the maximum samples are used, then leave the vial position after the last sample empty. The autosampler will stop sampling when it reaches on empty vial position Issue th March 2012 ECN

19 6.0 Operating Instructions 6.3 Switching on the Instrument The instrument will only operate when the plastic Safety Cover is fitted. Having setup the sampling method and loaded the sampling tray as described in sections 6.1 and 6.2, switch on the instrument with the on/off switch located at the lefthand side of the rear panel. The instrument will initialise itself, followed by the tray rotating to the parked position. The Probe will then be in the rest position. The READY LED will light, showing that the instrument is ready and waiting for the RUN key to be pressed. 6.4 Blanking and Calibration of the Flame Photometer Blanking with the Diluter (dipswitch 7 Off upper position) Press the RUN key on top of the Autosampler. The READY LED will flash showing that the instrument is waiting for the signals from the Flame Photometer for the Blanking and Calibration protocols. Switch on the Diluter and wait for the Lithium Blank (Carmine Red) to be seen steadily in the flame inspection port. Press the BLANK control on the Flame Photometer and the Sample Tray will rotate to present the Blank Vial to the Autosampler Probe, which will immediately descend and the Diluter will extract the Blank Solution through the Sample Tube and Autosampler Probe. After 30 seconds (or as modified by the DIP switch) the Autosampler Probe will exit the Blank Solution Vial and position itself above the Standard Solution Vial. At the same time the Blank Solution sample will be just entering the Diluter and a signal is sent to the Flame Photometer and the Blanking protocol is carried out by the Flame Photometer. If, for any reason, the Flame Photometer gives an error message during the Blanking procedure, you must press STOP on the Autosampler and then READY to reset the Autosampler. The Autosampler will now be ready for you to reattempt the Blanking of the Flame Photometer. Similarly, should the Autosampler fail for any reason, once it has been reset, you will need to restart the Flame Photometer sequence by pressing the BLANK key until it beeps and then repeat the Blanking procedure Issue th March 2012 ECN

20 6.0 Operating Instructions Blanking without the Diluter (dipswitch 7 On lower position) Press the RUN key on the top of the unit. The READY LED will flash showing that the instrument is waiting for the signals from the Flame Photometer for the Blanking and Calibration protocols. Press the BLANK control on the Flame Photometer and the Sample Tray will rotate to present the Blank Vial to the Autosampler Probe, which will immediately descend and a signal is sent to the Flame Photometer to prepare to receive the solution and start the Blanking sequence. If for any reason, the Flame Photometer gives an error during the Blanking procedure, you must press STOP on the Autosampler and then READY to reset the Autosampler. The Autosampler will now be ready for you to reattempt the Blanking of the Flame Photometer. Similarly, should the Autosampler fail for any reason, once it has been reset, you will need to restart the Flame Photometer sequence by pressing the BLANK key until it beeps and then repeat the Blanking procedure Calibration with the Diluter (dipswitch 7 Off upper position) Once the second SET button is pushed on the Flame Photometer (signifying acceptance of the calibration value) the Autosampler Probe enters the Standard Vial. After 30 seconds (or modified time) the Autosampler Probe exits the Standard Vial and positions itself over the first Sample Vial. The Standard solution will be just entering the Diluter and a signal is sent to the Flame Photometer for the Calibration protocol to be carried out by the Flame Photometer. After the calibration sequence is complete, the Model 860 waits for the Measure signal from the Flame Photometer. If for any reason, the Flame Photometer gives an error during the Calibration procedure, you must press STOP on the Autosampler and then READY to reset the Autosampler. The Autosampler will now be ready for you to reattempt the Blanking and then Calibration of the Flame Photometer. Similarly, should the Autosampler fail for any reason, once it has been reset, you will need to restart the Flame Photometer sequence by pressing the BLANK key until it beeps and then repeat the Blanking and Calibration procedures Issue th March 2012 ECN

21 6.0 Operating Instructions Calibration without the Diluter (dipswitch 7 On lower position) Once the second SET button is pushed on the Flame Photometer (signifying acceptance of the calibration value) the Autosampler Probe enters the Standard Vial. Immediately a signal is sent to the Flame Photometer which will initiate the calibration sequence. After 30 seconds (or modified time) the Autosampler Probe exits the Standard Vial and positions itself over the first Sample Vial. If for any reason, the Flame Photometer gives an error during the Calibration procedure, you must press STOP on the Autosampler and then READY to reset the Autosampler. The Autosampler will now be ready for you to reattempt the Blanking and then Calibration of the Flame Photometer. Similarly, should the Autosampler fail for any reason, once it has been reset, you will need to restart the Flame Photometer sequence by pressing the BLANK key until it beeps and then repeat the Blanking and Calibration procedures. 6.5 Running the samples Using the Diluter (dipswitch 7 Off upper position) The samples are measured in sequence following the press of the MEASURE button on the Flame Photometer. The first sample is aspirated for 30 seconds (or as modified by DIP switch settings, see section 6.1). After 30 seconds, when the sample will be just entering the Diluter, a signal is sent to the Flame Photometer and the Sample Measurement protocol is carried out by the Flame Photometer. After a delay of 10 seconds the Autosampler Probe automatically enters the next sample. Sample is drawn through the tube by the Diluter. Again the M860 waits until that sample just enters the Diluter before the Measure protocol is initiated. These actions are repeated automatically for each sample in turn until the M860 Sample Detection Probe senses that there are no more samples. (This could be at the end of the entire carousel of 40 samples or before). The M860 Autosampler Probe and Carousel then return to their initial position and the M860 sounds a buzzer to signify the completion of the analysis. Press the STOP/CLEAR button on top of the Autosampler tower to switch off the signal. At the sound of the buzzer wait after the last sample is measured before the diluter is switched off Issue th March 2012 ECN

22 6.0 Operating Instructions Without a Diluter (dipswitch 7 On lower position) The samples are measured in sequence following the press of the MEASURE button on the Flame Photometer. The first sample is aspirated for 30 seconds (or as modified by the dipswitch settings, see section 6.1). Immediately a signal is sent to the Flame Photometer and the Sample Measurement protocol is carried out by the Flame Photometer. After a delay of 10 seconds, the Autosampler Probe automatically enters the next sample and the next sample is aspirated for 30 seconds. Again the Autosampler send a signal and the next measure protocol is initiated. These actions are repeated automatically for each sample in turn until the M860 Sample Detection Probe senses that there are no more samples. (This could be at the end of the entire carousel of 40 samples or before). The M860 Autosampler Probe and Carousel then return to their initial position and the M860 sounds a buzzer to signify the completion of the analysis. Press the STOP/CLEAR button on top of the Autosampler tower to switch off the signal Aborting a Sample Run Sampling can be aborted at any time by pressing the STOP/CLEAR key on the top of the Autosampler. The instrument will continue to measure the sample already in the diluter then return to the parked position. The READY LED will light, showing that the instrument is waiting for the RUN key to be pressed. If a set of samples is interrupted, either by pressing the STOP key or by a fault condition occurring, and after the end of a completed run, Blanking and Calibration will be required before the samples can be run again or a new set of samples can be measured Issue th March 2012 ECN

23 6.0 Operating Instructions 6.6 Fault Conditions If an instrument fault occurs or the safety cover is removed during operation, all movements will stop immediately and the FAULT led will flash. The READY, STATUS and SAMPLING LED will light according to the fault condition as follows: READY SAMPLING STATUS Initialisation Error ON OFF Flashing Movement Error OFF ON Missing Blank or OFF OFF Flashing Standard Vial Safety cover off, OFF OFF Flashing System error ON ON If an error occurs then either, press the STOP/CLEAR key on the top of the instrument which will attempt a reinitialisation, or switch the instrument off and correct the cause of the error before switching back on again. If the error persists, please contact your supplier Issue th March 2012 ECN

24 7.0 Replacement Procedures 7.1 Autosampler Probe Replacement Issue th March 2012 ECN

25 8.0 Appendix A 1:50 Dilution The Model 860 Autosampler is supplied as standard with two packs of three tubes: Tube, Manifold, Internal Standard, pack of three Tube, Manifold, Red, Long, pack of three The Internal Standard Tube, , (Orange wishbone) is the one to be used normally when connecting the Autosampler Probe either to the Manifold Block of the M805 Dilutor or directly to the inlet of the Nebuliser of the Flame Photometer. That tube and its use are referred to in the main text of the Operator Manual. When used with the Model 805 Dilutor, standards and samples will be automatically diluted 1 part in 200. When used directly between the Autosampler and the Nebuliser on the Flame Photometer, the sample uptake rate will be optimal. The larger internal bore tube (Red wishbone) will provide a 1 part in 50 dilution when using the Model 860 Autosampler with the Model 805 Dilutor. It cannot be used to connect the Autosampler directly to the Nebuliser on the Flame Photometer. The vast majority of users will not need/want to use the larger internal bore tube. 8.1 Operating the Model 860 with tube. The settings/timings given in step 1 and 2 below are suggested starting points only. Each individual system may have to be adjusted as necessary to give best results. 1. The dipswitches 1, 2, and 3 on the back of the Autosampler need to be set to ON to give a delay of 36 seconds from the Autosampler. 2. The delay time in the M420/425 Flame Photometer needs to be set to 20 seconds. 3. The tube, which has a red wishbone ( ), should be connected to the Diluter Manifold using the metal tube nearest the dilutor body. The tube must be pushed all the way over the metal manifold tube so that it comes to a stop up against the manifold body. 4. The Internal Standard Tube (Orange wishbone ) should be connected to the Diluter Manifold using the metal tube furthest away from the diluter body, passed through the dilutor body and the end placed in the Lithium Internal Standard Issue th March 2012 ECN

26 8.0 Appendix A 1:50 Dilution 5. The 1:50 tube must be pushed onto the Autosampler Probe so that it goes over the outer (thicker) metal probe sheath. This is achieved most easily by twisting the tube around between thumb and finger whilst pushing the tube onto the probe. 6. With the Dilutor switched on, a constant stream of bubbles observed in the tubing, means the tubing should be pushed further over the thicker outer sheath of the Autosampler Probe. If that fails to make any difference, the seal between the inner and outer tube of the probe has failed and needs to be resealed. 7. When pulling the 1:50 tube off the probe, the tube should be twisted and only when the tube has started to rotate around the outside of the metal probe should the tube be pulled from the probe. Pulling the tube straight from the probe without twisting may damage the probe and/or the alignment of the probe driver assembly Issue th March 2012 ECN

27 9.0 Appendix B Updating the Firmware The microprocessor fitted to the M860 has a feature which allows the firmware to be updated via the RS232 communications port, eliminating the need to remove the microprocessor. It requires that the Atmel Flip Utility be downloaded from the Atmel website to your pc/laptop. or search for Atmel Flip. (This is a free download). Download Atmel Flip or later and start the application. Connect the M860 to the RS232 port of your computer using the Null Modem Cable ( ) supplied with the M860. Move the two DIP switches located at the bottom righthand side on the front of the M860 to the down position and switch on the instrument. On the Flip utility select the device type AT89C51ED2. DEVICE> SELECT. Set the communications to RS232. SETTINGS> COMMUNICATIONS. Load the new firmware obtained from your Sherwood distributor. FILE> LOAD HEX FILE. When the update is completed, switch off the M860 and return the two DIP switches to the original up position Issue th March 2012 ECN

28 Sherwood Scientific Limited Product Warranty Statement Warranty Term: 12 Months Sherwood Scientific Ltd (Sherwood) warrants, subject to the conditions itemised within this document, through either Sherwood personnel or personnel of its authorised distributors, to repair or replace free of all charges, including labour, any part of this product which fails within the warranty time specified above, appertaining to this particular product. Such failure must have occurred because of a defect in material or workmanship and not have occurred as a result of operation of the product other than in accordance with procedures described in the instructions furnished with this product. Conditions and specific exceptions that apply to the above statement are as follows: 1. Enduser warranty time commences on the date of the delivery of product to enduser premises. 2. Free of all charges statement applies only in areas recognised by Sherwood as being serviced either directly by its own personnel, or indirectly through personnel of an authorised distributor. Products purchased outside these areas requiring service during the warranty period will incur charges relative to the travel/transit costs involved. However, products purchased in such areas will be serviced during the warranty period free of all charges providing they are returned, carriage paid, to either Sherwood or by prearrangement to an authorised Sherwood distributor. 3. All maintenance (other than operator maintenance as described in the instructions), repairs or modifications have been made by Sherwood or Sherwood authorised personnel. 4. This product has where applicable been operated using Sherwood specified supplies and reagents. 5. Sherwood reserves the right to make any changes in the design or construction of future products of this type at any time, without incurring any obligation to make any changes whatsoever to this particular product. 6. Reagents, supplies, consumables, accessories and user maintenance items are not included in this warranty Issue th March 2012 ECN

29 Product Warranty Statement () 7. Repairs or replacement of any part failing due to abnormal conditions including the following, are excluded from this warranty: a. Flood, lightning, earthquake, tornado, hurricane, or any other natural or manmade disaster. b. Fire, bombing, armed conflict, malicious mischief or sprinkler damage. c. Physical abuse, misuse, sabotage or electrical surge. d. Damage incurred in moving the product to another location. 8. User agrees to permit Sherwood personnel or personnel of its authorised distributor to make changes in the product which do not affect results obtained, but do improve product reliability. Representations and warranties purporting to be on behalf of Sherwood made by any person, including distributors and representatives of Sherwood, which are inconsistent or in conflict with the terms of this warranty (including but not limited to the limitations of the liability of Sherwood as set forth above), shall not be binding upon Sherwood unless reduced to writing and approved by an officer of Sherwood Scientific Ltd. Except for the obligations specifically set forth in this warranty statement, in no event shall Sherwood be liable for any direct, indirect, special, incidental, or consequential damages, whether based on contract, tort or any other legal theory and whether advised of the possibility of such damages. Neither Sherwood nor any of its third party suppliers makes any other warranty of any kind, whether expressed or implied, with respect to Sherwood Products. Sherwood Scientific Ltd., 1 The Paddocks, Cherry Hinton Road, Cambridge, CB1 8DH, England Issue th March 2012 ECN