File No. 28(1192)/2017/Pur Date: 19/06/2018

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Alicia Dorsey
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1 File No. 28(1192)/2017/Pur Date: 19/06/2018 Revised Technical Specifications and Clarifications issued after the Pre-Bid Conference Dated for SUPPLY, INSTALLATION AND COMMISSIONING OF PARTICLE SIZE AND ZETA POTENTIAL MEASUREMENT ANALYZER PARTICLE SIZE AND ZETA POTENTIAL MEASUREMENT ANALYSER-SPECIFICATIONS Main Features and Specifications Parameters Temperature control range Purging Standard laser Laser Power C Dry air purge Any one laser within the range of nm may be supplied 4mW Size measurement Dynamic Light Scattering Range (diameter) 0.3 nm-10 µm Minimum sample volume 20 µl Minimum concentration Maximum concentration Measurement angle Usable liquids 0.1 % w/v or better 40 % w/v Back scattering angle Aqueous, organic solvents Zeta potential Measurement range Measurement conductivity range Laser Doppler Electrophoresis -200 to +200mV 20 S/m

2 Maximum sample concentration Minimum sample volume 40% w/v 1000µL and along with optional accessories (cuvettes and sample holders) to handle less than 500 µl volume Molecular weight Molecular weight range Desirable Debye plot method or Back Scattering Angle method 1000 Da- 2*10 7 Da Supporting application lab in India Other features/general requirements Detector type may be of PMT or Photo diode/avalanche Photodiode Warranty : Three Years (Comprehensive) Necessary Power adapters/spike busters, and devices necessary in tune with the specifications listed above Start up/installation kit and manual Installation, comprehensive demonstration and training Desktop Computer-Specifications The configuration shall be viz. Branded PC (Preferably Dell/HP) with Intel core i7 (latest generation) 16 GB RAM, 1TB 7200 RPM 3.5 SATA Hard Drive 16 Max DVD +/- RW MT Integrated Intel HD Graphics card (latest) USB Optical Mouse USB Entry Business Keyboard (English) Compatible latest Windows OS 27 LED Monitor Necessary connecting ports for control/operation of the instrument

3 CLARIFICATIONS Firm Name: M/s. ADVANCE SCIENTIFIC EQUIPMENT PVT. LTD. Parameter Tender Specification Requesting to modify as Standard laser 633/532/660nm nm Minimum sample volume 100μL 750 μl to 1ml or better Laser: Standard laser: Any one laser within the range of nm may be supplied Zeta Potential: Minimum sample volume: 1000 µl and along with optional accessories to handle less than 500 µl volume Firm Name: M/s. Aimil Ltd Molecular weight measurement We kindly request include Scattering angle for Molecular weight measurement. To eliminate the multiple scattering peaks due to concentration dependence as different angles are linked to different positions scattering angle plays a major role. We recommend Backscattering angle. Debye Plot Method or Back Scattering Angle method

4 Firm Name: M/s. Anton Paar India Pvt Ltd Detector Photo Diode or Avalanche Photo Diode. Photo Diodes are of latest generation more sensitive by times the PMTs. Photo Multiplier Detectors (PMT) are convectional detectors that suffer from higher dark currents that limits the sensitivity of the systems to work with low concentrations and weekly scattering materials like Biological, Nano particles <30 nm in size. In addition to PMT, Photo Diodes or Avalanche Photo Diodes in the specification for detector is also included Question 2 Sensitivity / Minimum sample concentration Limits for Size & Zeta Potential : Question 3 Size Measurement Minimum Sample Volume: 20 µl Zeta Potential Measurement Minimum Sample Volume: 1000 µl and along with optional accessories to handle less than 500 µl volume Zeta Potential range: mv to mv. Majority of DLS instruments offer equal or more than: ± 500 mv. The mentioned specification of mv to mv can be insufficient considering future application needs of developing sophisticated materials. Do not see any significance in having zeta potential values beyond the -200 to +200 range Firm Name: M/s. Labindia Instruments Pvt Ltd Min sample volume 15µL We request Min Sample volume should be 20µL and low. Size Measurement Minimum Sample Volume: 20 µl Question 2 Laser Doppler Electrophoresis We are having Electrophoretic Light Scattering principle to measure zeta potential. I request you to give option for Electrophoretic Light Scattering principle also.

5 Both the techniques are one and the same Question 3 Minimum sample volume 100µL With our instrument min Sample volume is 130 µl. We request you to keep min sample volume 130 µl and low Zeta Potential Measurement Minimum Sample Volume: 1000 µl and along with optional accessories to handle less than 500 µl volume The tender specification would stand changed to the above extent. Tender Due Date & Time: 12 th July 2018 up to 3.00 P.M (IST) Technical Bid Opening Date & Time: 13 th July 2018 up to 3.00 P.M (IST)

INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH PUNE

INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH PUNE CLARIFICATION ON TENDER NUMBER - IISER-PUR-0284-17 ITEM DESCRIPTION- PROCUREMENT OF GRAPHITE-FURNACE ATOMIC ABSORPTION SPECTROMETER WITH CHNS/O ANALYZER