Microbiological Safety Cabinet

Transcription

1 Microbiological Safety Cabinet Installation & User Manual Class II Type A2 Type 4 feet 5 feet 6 feet Window operation height Touch panel /115V QSD YA USTP204 QSD YA USTP205 QSD YA USTP206 20CM 60Hz 10A QSD YA USTP254 QSD YA USTP255 QSD YA USTP256 25CM Touch Panel/ 230V 50/60hz 7A QSD YA BCTP204 QSD YA BCTP205 QSD YA BCTP206 20CM QSD YA BCTP4 QSD YA BCTP5 QSD YA BCTP6 25CM Membrane switch panel/ 115V 60hz 10A Membrane switch panel/ 230V 50hz 7A QSD YA USSZ420 QSD YA USSZ520 QSD YA USSZ620 20CM QSD YA USSZ425 QSD YA USSZ525 QSD YA USSZ625 25CM QSD YA BCSZ420 QSD YA BCSZ520 QSD YA BCSZ620 20CM QSD YA BCSZ425 QSD YA BCSZ525 QSD YA BCSZ625 25CM

2 Version 1.3, May,2014 All rights reserved.

3 Foreword Thank you for purchasing a STERIZONE Class II Type A2 Microbiological Safety Cabinet. Your STERIZONE Microbiological Safety Cabinet has been tested in accordance to EN It is designed to protect the operator, the product and the lab environment from biohazardous experiments by providing a sterile and stable airflow inside the cabinet. The STERIZONE MSC offers a number of unique features to enhance your safety, performance and operate more efficiently. Please read this manual carefully before installation, use, trouble shooting and maintenance for proper application of the cabinet. Also, keep this manual with factory test report near the cabinet for convenient reference for operators and qualified maintenance personnel. If you have any questions or technical assistance, please feel free to contact us. 570 Alden Road, Markham Ontario L3R 8N5, Canada Tel: Fax: qsr@qsrlab.com

4 Table of Contents I. General Instruction... 1 II. Installation Locate Cabinet Assemble Foot Stand Connect Service Valve Connect Service Lines Install Drain Valve Connect Power Performance Test after Cabinet Installation III. Cabinet Description Specification Cabinet Air Flow IV. Control Panels Instruction Membrane Switch Panel V. Operation Operation Procedure for Membrane Switch Panel Good Ergonomics Cleaning, Disinfection & Decontamination VI. Maintenance Calibration of Airflow Inflow Velocity Measurement Fluorescent Lamp Replacement UV Lamp Replacement... 28

5 6.5 HEPA Filters Replacement Blower Replacement Maintenance Schedule Labels VII. Trouble Shooting VIII. Warranty... 39

6 I. General Instruction Microbiological Safety Cabinet are classified to three types:class I Class II Class III. US National Sanitation Foundation (NSF) had issued Std.49 for Class II Microbiological Safety Cabinet. It can assure the MSC design, performance and quality and as a guide book for professional researcher use. Class II MSC has A1 A2 B1 B2 types, which can protect the operator, environment and the biological materials from cross-contamination. The comparison for all types of Class II MSC is as Table 1.1 Table 1.1 Types of Class II MSC Type Inflow velocity (m/s) Air flow circulation Position of exhaust vent A A B B % exhaust 70% circulation 30% exhaust 70% circulation 70% exhaust 30% circulation 100% exhaust 0% circulation Indoors / Outdoors Indoors / Outdoors Outdoors Outdoors Air flow must pass through window opening to go from outside to the operation area. It can prevent the aerosol contaminate the operator, the exhaust air flow go to the inlet of the circulation channel. Then go to the blower fan and force the air to HEPA filter (filtering efficiency for 0.3µm is 99.99%). After filtering, the clean air goes to exhaust vent and/or re-circulate to the operation chamber area. HEPA filter can filter the air borne particle or pathogen aerosol. But the HEPA filter can not filter the organic volatile gas or chemicals. It is strongly recommended that use the suitable venting system or cyclone of chemical hood to vent the hazard toxic chemicals. All the types of class II biosafety cabinets can be used for biosafety level 1~3 laboratories. It can offer clean environmental area for cell culture operation and anticancer chemotherapy drugs operation. Our MSC is Class II type A2(30% exhaust, 70% circulation).the main characteristics for class II type A2 biosafety cabinet are: 1. Inlet supply air must at least maintain 0.5 m/s air flow. (window sash height 20 cm.) 1

7 2. Downward air flow to the operation area must go through HEPA filter first. Then discharge or exhaust to the vent. 3. The cabinet chamber must maintain negative pressure to prevent hazard substance leak to outside. STERIZONE MSCs are designed to assure all chambers of the cabinet are in negative pressure by double layers of leak proof tunnel bodies which include operation area, bottom of the body, lateral bodies and dorsal bodies. All the materials are made of stainless steel #304. It is easy to clean and maintain. The tunnel is designed to round angle and no death space to reduce the air flow resistance. The exhaust venting system of external hood cover and hard tubing is option accessories. Choose the suitable, verified and certified MSC according to the biosafety laboratory level is the best policy for the safety. ~ Your air, our care ~ 2

8 II. Installation 2.1 Locate Cabinet The ideal location for the cabinet is as follow Fig It must be away from the traffic pattern, passage, door, fuming hood, air conditions and other air turbulence locations. If there are windows in the lab, they should remain closed at all time. Room air flow turbulence will interfere the MSC laminar flow and let the contaminate flow out to the MSC outside. It will be harmful to the operators and affect the lab environment quality. Biosafety Cabinet Biosafety Cabinet Door Supply Air Inlet Supply Air Inlet Biosafety Cabinet Fig. 2.1 Location Recommended for Biosafety Cabinets It is recommended to locate the MSC for sparing at least 30 cm (12 inch) clearance on each side. If the clearance not available, a minimum 8 cm (3 inch) clearance on each side and 4 cm (1.6 inch) clearance in back are recommended. Also, with at least 8 cm (3 inch) clearance to the ceiling for the exhaust opening at the top of the cabinet, it won t squeeze the exhaust airflow and decrease inflow at the front opening for the cabinet. The power for STERIZONE MSC is single-phase 115V@60Hz or 230V@50-60Hz. Independent single socket usage, UPS and earth connection are suggested. Don t set up the MSC in unstable cart or table. 3

9 2.2 Assemble Foot Stand The cabinet can be placed on a stable structure or a foot stand provided. The foot stand and cabinet body is detachable and easy for shipping and assembly. For a unit with a stand, assemble the stand first, and then place the unit on the stand. Caution: The BSC s weight is more than 300 kg. It must be moved very carefully. 1. Use 3/8 x (2-1/2) long hex screw with a 3/8 flat washer to connect the right and left side stand frame to the rear crossmember. Tighten the screws securely. See Fig Use 3/8 x (2-1/2) long hex screw with a 3/8 flat washer to connect the right and left side stand frame to the central crossmember. Tighten the screws securely. 3. Use 3/8 x 5/8 long hex screw with a 3/8 spring washer and 3/8 flat washer to connect the diagonal brace to the stand frame and rear crossmember. Tighten the screws securely. 4. Move the foot stand completed to the final location and adjust the levelers. Now, connect the cabinet to the foot stand. See Fig Place the cabinet onto the foot stand carefully and slide it into position. 2. Insert 3/8 x (2-1/2) long hex screw with a 3/8 spring washer and 3/8 flat washer through the holes on the top of frame and bottom of the cabinet, tighten screw with a 3/8 nut securely. Table 2.1 Accessories for foot stand assembly Item Description Qty Item Description Qty 1 3/8 x(2-1/2) long hex screw 6 10 Central crossmember 1 2 3/8"X5/8"long hex screw 4 11 Rear crossmember 1 3 3/8" washer Diagonal brace 2 4 M6 washer 8 13 L-type bracket 4 5 3/8 spring washer 12 6 M6 spring washer 8 7 M6x16 Round screw 8 8 Left side stand frame 1 4

10 9 Right side stand frame Fig

11 4 6 Leveler 7 Fig

12 2.3 Connect Service Valve The valves are designed for the following service applications for air, gas and vacuum. Maximum recommended service pressure is 30 psi. Two service valves can be installed on the right/left side of the unit. Service valve fixtures can be ordered factory installed or as options and installed on-site. Refer Fig. 2.4 to connect the valve. 1. Remove the cover on side plate. Fig Remove the service valve plug. 3. Install the valve and secure. 7

13 2.4 Connect Service Lines Follow the steps below to connect the service lines. 1. Remove the cover on the side plate. 2. Route the service line from the bottom of the cabinet side plate. 3. Connect the service line to the connector securely. Service line connector NOTE: For safety, easily assessable shut-off valves and pressure regulators should be equipped on the service lines. Caution: Avoid using open flames, high pressure gases and flammable solvent in the cabinet. It will disrupt the airflow patterns and damage the filters. 8

14 2.5 Install Drain Valve The valve should be assembled after the cabinet has been secured to the foot stand. See Fig Wrap the thread seal tape in the same direction the threads run around the top of the hose connector with 10 loops. NOTE: Wrapping the wrong way may result in the tape coming unwound as the fittings are tightened. 2. Joint the hose connector and ball valve, then tighten. 3. Wrap the thread seal tape in the same direction the threads run around the bottom of the drain outlet with 10 loops. 4. Connect the ball valve assembly to the drain outlet securely. Make sure the valve handle faces the front of the cabinet. 5. Connect a drain hose to the hose connector if necessary. Drain outlet Ball valve Close Hose connector Open Fig

15 2.6 Connect Power The power for STERIZONE MSC is single-phase or Before connect the plug to the outlet, verify the voltage specification of the unit and check the matches of the outlet. Caution: To avoid the risk of electrical shock, make sure that the dedicated outlet is grounding. And, do not remove the grounding prong of the unit power plug. 2.7 Performance Test after Cabinet Installation According to NSF/ANSI , Annex F, the following tests related to containment and product protection should be conducted on-site after the MSC being installed to ensure that the functions meet the criteria. 1. HEPA Filter Leak Test 2. Down Flow Velocity Test 3. Inflow Velocity Test 4. Airflow Smoke Pattern Test 5. Site Installation Assessment Tests 10

16 III. Cabinet Description 3.1 Specification No. Description No. Description 1 Exhaust louver 9 Work surface 2 Exhaust HEPA filter 10 Frontal plate 3 Blower 11 Window sash 4 Supply HEPA filter 12 Front air intake grill 5 Control plate 13 Armrest 6 Cabinet body 14 Drain valve 7 Electrical outlet 8 Service valve 11

17 QSD YA QSD YA QSD YA QSD YA Model No. USTP254 BCTP4 USSZ425 BCSZ425 External Dimensions W133 D79 H154 cm Support Stand Height 66 cm Internal Dimensions W122 D63 H75 cm Cabinet Construction Stainless steel #304 interior and work surface Control Panel touch panel membrane switch single-phase single-phase single-phase single-phase 115V/60Hz/10A 230V/50-60Hz/7A 115V/60Hz/10A 230V/50-60Hz/7A Electrical Main supply power fluctuations not to exceed ±5% of the nominal voltage. Requirements A voltage stabilizer is recommended. Same voltage for the main input power and the outlets inside the unit. Window Type Window Operation Height Average Inflow Velocity Average Downflow Velocity Supply/Exhaust HEPA Filter Efficiency Max. Opening Height of Window Sash Light Intensity Acceptable Options Room Height Suggested Net Weight Environmental Conditions sliding 20 cm (8 inch), 25 cm (10 inch) 0.51 ~ 0.56 m/s (100~110 fpm) 0.3 ~ 0.35 m/s (59~68 fpm) 99.99% at 0.3 micron 60 cm (23.6 inch) >1,000 lux I.V. Pole and Exhaust Canopy 227cm at least 269kg (593 lbs) Indoor use only. Up to 2,000 meters (6,500 ft) above sea level. Room temperature 20 to 30. Relative humidity up to 80%. 12

18 Model No. QSD YA QSD YA QSD YA QSD YA USTP255 BCTP5 USSZ525 BCSZ525 External Dimensions W163 D79 H154 cm Support Stand Height 66 cm Internal Dimensions W152 D63 H75 cm Cabinet Construction Stainless steel #304 interior and work surface Control Panel touch panel membrane switch single-phase single-phase single-phase single-phase 115V/60Hz/10A 230V/50-60Hz/7A 115V/60Hz/10A 230V/50-60Hz/7A Electrical Main supply power fluctuations not to exceed ±5% of the nominal voltage. Requirements A voltage stabilizer is recommended. Same voltage for the main input power and the outlets inside the unit. Window Type Window Operation Height Average Inflow Velocity Average Downflow Velocity Supply/Exhaust HEPA Filter Efficiency Max. Opening Height of Window Sash Light Intensity Acceptable Options Room Height Suggested Net Weight Environmental Conditions sliding 20 cm (8 inch), 25 cm (10 inch) 0.51 ~ 0.56 m/s (100~110 fpm) 0.25 ~ 0.30 m/s (59~68 fpm) 99.99% at 0.3 micron 60 cm (23.6 inch) >1,000 lux I.V. Pole and Exhaust Canopy 227cm at least 312kg (688 lbs) Indoor use only. Up to 2,000 meters (6,500 ft) above sea level. Room temperature 20 to 30. Relative humidity up to 80%. 13

19 Model No. QSD YA QSD YA QSD YA QSD YA USTP256 BCTP6 USSZ625 BCSZ625 External Dimensions W193 D79 H154 cm Support Stand Height 66 cm Internal Dimensions W182 D63 H75 cm Cabinet Construction Stainless steel #304 interior and work surface Control Panel touch panel membrane switch single-phase single-phase single-phase single-phase 115V/60Hz/15A 230V/50-60Hz/10A 115V/60Hz/15A 230V/50-60Hz/10A Electrical Main supply power fluctuations not to exceed ±5% of the nominal voltage. Requirements A voltage stabilizer is recommended. Same voltage for the main input power and the outlets inside the unit. Window Type Window Operation Height Average Inflow Velocity Average Downflow Velocity Supply/Exhaust HEPA Filter Efficiency Max. Opening Height of Window Sash Light Intensity Acceptable Options Room Height Suggested Net Weight Environmental Conditions sliding 20 cm (8 inch), 25 cm (10 inch) 0.51 ~ 0.56 m/s (100~110 fpm) 0.25 ~ 0.30 m/s (59~68 fpm) 99.99% at 0.3 micron 60 cm (23.6 inch) >1,000 lux I.V. Pole and Exhaust Canopy 227cm at least 355kg (783 lbs) Indoor use only. Up to 2,000 meters (6,500 ft) above sea level. Room temperature 20 to 30. Relative humidity up to 80%. 14

20 Replacement Parts Part No. Description BB-0086-A UV Lamp- T8 30W BB-RA-B6204-A Fluorescent Lamp- T5 28W AH0-145-T02-B Supply HEPA Filter AHA-133-T001-A Exhaust HEPA Filter BB-GE-ECM-30-A Motor- Single-phase, 1/2HP 120V,60Hz,7.7A/240V,50Hz,4.3A 200~1,300 rpm BB-GE-ECM-32-A Motor- Single-phase, 3/4HP 120V,60Hz,10.1A/240V,50Hz,6.1A 200~1,300 rpm 15

21 3.2 Cabinet Air Flow 1. Room air is pulled through the work surface grille, then enter the negative plenum and reach the blower plenum. The air is drawn by the blower and passed through the supply HEPA filter and exhaust HEPA filter with a ratio of about 70% for supply filter and 30% for exhaust filter. 2. The air filtered through supply HEPA filter flows down to the work area as a sterile laminar flow air stream. Also, the filtered and sterile air is exhausted into the room or exhaust system (if the exhaust canopy assembled). 3. At the work surface, the air is split up into two streams. A portion is drawn into the front grille and the rest drawn into the rear grille. A small portion is drawn into the work surface side and window side wall intake slots. 4. The air mixing room air and the contaminated air which flows through the work area is pulled through the negative plenum and blower again. The process of 30% exhausted and 70% recirculated is continued. 16

22 IV. Control Panels Instruction 1 We provide two options for control panel for STERIZONE MSC. One is Touch Panel and the other one is Membrane Switch Panel. 4.1 Membrane Switch Panel The membrane switch panel is installed on our STERIZONE USSZ/ BCSZ series biosafety cabinets. It provides an easier interface to operate the unit which has the same performances as the unit with touch control panel. 1. POWER ON/OFF Turn the key to control the unit POWER on and off. 2. FLUORESCENT LIGHT ON/OFF Press the button to control the FLUORESCENT LIGHT on and off. It interlocks with the WINDOW, BLOWER and UV LIGHT. It will be activated automatically when the WINDOW is opened, and shut off automatically when the WINDOW is fully closed. 3. BLOWER ON/OFF Press the button to control the BLOWER on and off. It interlocks with the WINDOW, FLUORESCENT LIGHT and UV LIGHT. It will be activated automatically when the WINDOW is opened, and shut off automatically when the WINDOW is fully closed. 4. OUTLET ON/OFF Press the button to control the OUTLET on and off. There are electrical OUTLETS with splash-proof cover installed inside the unit. The voltage for the OUTLETS is the same as the main input power. It provides an electrical source for the devices needed during experiment period. 17

23 5. UV LIGHT ON/OFF Press the button to control the UV LIGHT on and off. It interlocks with the WINDOW, FLUORESCENT LIGHT and BLOWER. It will be activated automatically when the WINDOW is fully closed, and shut off automatically when the WINDOW is opened. Built-in UV LIGHT timer for 60 minutes. The timer can be reset for 0~99 minutes or hrs. 6. MUTE When the alarm activated, it can be muted temporarily (5 minutes) by pushing the MUTE switch. At the same time, the ALARM LIGHT continues to flash. Once the abnormal situation has not been settled, the AUDIBLE ALARM will be activated again after the setting time (5 minutes) to remind the user. The factory s mute setting time is 5 minutes, it can be reset by the user. 7. ALARM When the WINDOW is not at the operation height set, the ALARM LIGHT will be activated and release an audible automatically. 8. MAGNEHELIC GAUGE It displays the static negative pressure in the unit of mm Aq. The negative pressure shall be not less than 8.3 mmaq. 18

24 V. Operation MSC must be operated by well trained and qualified person. Before operation, read the user manual first, and confirm the models which you purchase. 5.1 Operation Procedure for Membrane Switch Panel 1. Pre-planning Completely understand whole operation procedures and required objects before the operation. Minimize the factors will break down the operation of Microbiological Safety Cabinet, such as personnel movement. 2. Disinfection before operation Turn off the UV light and raise the window to the maximum opening height. The UV light will be off automatically (if it was activated) once the window being opened. The interior surface of the unit is wiped with 70% alcohol thoroughly. If the disinfection is performed with chloride or halogen chemicals, the stainless steel surface can be wiped with alcohol to prevent the steel surface from damage. 3. Load materials Cover the working tray with anti-infected paper towel or pad if needed. Prepare the disinfectant solution, paper towel and alcohol cotton swab and refuse container. Only load objects needed in this experiment, and take excessive objects out the unit. Keep front and back air intake slots unobstructed. Keep large objects apart from each other. After complete of placement, blow the object surface with airflow for 2 ~ 3 minutes to take away the particles on it. 4. Warm up Turn the power key to the ON position. Press the fluorescent light and blower buttons at I position to enable the function. NOTE: The fluorescent light and fan will not be enabled when the window is fully closed although the switches are on. Move the window to the operation height (20 cm or 25 cm). Close the drain valve. It must be closed before operation for preventing the contaminated liquid spilling during operation. Check the obstruction of each air intake slot, and pay attention to the magnehelic 19

25 gauge reading. The negative pressure reading should be not less than 8.3 mmaq. Run the unit for at least 5 minutes. The unit is ready for operation. 5. Personnel dress Wash thoroughly the fingers and arms. Wear long-type laboratory clothing with elastic sleeve and rubber glove. The glove should cover the sleeve of laboratory clothing. (Long-type laboratory clothing is recommended to reduce the falling of miscellaneous objects from hands to the cabinet, and protect hands from the hazard of test specimen.) Wear protective eyewear and mask if needed. 6. Work techniques Keep the distance between object and window at least 10 cm. Keep the clean objects apart from contaminated objects, and avoid the entrance of contaminated objects into clean zone. Place disposed and contaminated objects at the back of the unit work surface. During operation, avoid the movement of objects or excessive arm stretch in or out the window. Should not use open flame. Use applicable aseptic technique. Avoid the use of techniques or procedures will interfere with the airflow condition in the unit. In case of spillage or splash, the surface of all objects should be de-contaminated before being moved out of the unit. Disinfect the interior of cabinet under operation to protect from escape of contaminants. 20

26 Back Safe Operation Zone Front Air Intake Grill Don t operation here Front Fig. 5.1 Safe operation zone for the cabinet 7. Remove materials Disposable contaminated objects should be placed at the treatment plate or decontamination bag in the unit after use, such as gloves. De-contaminate the surface of reusable objects which contacted with contaminants before removing from the unit. Cap all the opened plates or containers before removing from the unit. NOTE: Never use the cabinet to storage lab equipment or supplies. 8. Disinfection after operation The interior surface of the unit is wiped with 70% alcohol. If the disinfection is performed with chloride or halogen chemicals, the stainless steel surface can be re-wiped with alcohol to prevent the stainless steel surface from damage. 9. Shut down Run the unit continuously for at least 5 minutes to remove residual air-borne contaminants in the operation area. Turn the fluorescent light and blower off, if necessary. Close the window. Turn the power key to the OFF position or turn the UV light on, if necessary. NOTE: UV light can be used as an adjunct surface decontamination procedure, but should 21

27 not be seen as a way that can replace good decontamination procedures. Turn on UV light for 30~60 minutes to decontaminate the interior surface of the unit. It should not be used for extended time to avoid shortening the lamp s lifespan. 5.2 Good Ergonomics Working in MSCs requires laboratory personnel to assume a variety of awkward postures due to limited work access, which restrict arm movement, and therefore significantly increase the amount of stress on joints of the upper limbs, neck, and back. The following are important considerations when attempting to work with the body in a comfortable and healthy posture to reduce stress and strain on the muscles, tendons, and skeletal system. 1. Prevent extended reaching, place materials as close as possible. 2. Perform your work at least 10 cm back into the cabinet to maintain optimal airflow containment for material and personal protection. 3. Always assume a proper posture. Use only adjustable chair or stool with built-in foot and arm rest. 4. Avoid contact pressure (forearm and wrists contact with sharp edges). Apply foam padding to the front sharp edge of the unit to reduce pressure concentration. 5. If you perform work in a cabinet while standing for prolonged periods of time, use an anti-fatigue mat and footrest to reduce muscle, joint, and spinal fatigue. 6. Take short breaks to alter repetitive forearm and wrist motion, relieve joint pressure and contact pressure caused by sharp edges. 7. Reduce eye strain and awkward posture by keeping viewing window of cabinet clean, and line of sight unobstructed. 8. Make sure the cabinet lighting is working properly. Good and proper lighting helps reducing eye strain. 9. Raise cabinet couple inches upwards to create a more comfortable leg and thigh clearance. 22

28 5.3 Cleaning, Disinfection & Decontamination Cleaning 1. Use a clean cloth with warm water or suitable detergent to wipe the interior and exterior surfaces of the unit including the window and work tray. 2. Dry all chamber surfaces with a clean cloth. Disinfection 1. Apply appropriate disinfectants to wipe down the cabinet surface. Usually, 70% alcohol is used as the disinfectant. 2. Wipe off excess and residue disinfectant, and allow the disinfectant to dry. NOTE: Avoid using the disinfectants with chloride or halogen chemicals, they will cause a corrosive and damage to the stainless steel surface. Decontamination Decontamination for the cabinet is a must when HEPA filter changes and maintenance or tests performance which require access to any contaminated areas of the unit. Please refer to NSF/ANSI , Annex G, for the specification of microbiological decontamination procedure with formaldehyde. Remember, the decontamination must be performed by well trained and qualified personnel. The following are the procedures recommended for the normal contamination treatment: 1. Wear the suitable protective device. 2. Allow the cabinet to run continuously. 3. Cover the contamination area with anti-infected paper towel or pad. 4. Spray suitable disinfectant on the paper towel. 5. Report to the lab or biosafety supervisor for further instruction. 6. If no special instruction order, wait for 30 minutes and clean the contamination area. 7. Dump the refuse by forceps to the suitable container. 8. Spray and wipe the contaminated area twice with disinfectant and paper towel. 9. Take off the outer gloves and dump it to the refuse container. 10. Autoclaving the refuse container. 11. After decontamination completed, dispose the refuse according to the local and national rules and policies. NOTE: For serious massive spill of contamination, all lab personnel must leave the lab immediately with allowing the cabinet to run continuously. Keep the warning sign on the door and report to the lab or biosafety supervisor for further professional treatments. 23

29 VI. Maintenance 6.1 Calibration of Airflow The nominal average downflow velocity is 0.3~0.35 m/s or 0.25~0.3 m/s and average inflow velocity is 0.51 ~0.56 m/s for STERIZONE MSCs. After the cabinet being used for a period of time, replacing a new HEPA filter or whenever you need the unit to be tested to check the airflow, it is necessary to calibrate the airflow when the measurements are not in the range of the nominal values. NOTE: The calibration of airflow must be operated by well trained and qualified personnel. We provide two methods to adjust and balance the airflow: Table 6.1 Adjustments specification for airflow Airflow Adjustments Inflow Downflow Motor speed Exhaust damper opening O.K. High Decrease Open O.K. Low Increase Close High High Decrease --- High Low --- Close Low High --- Open Low Low Increase --- High O.K. Decrease Open Low O.K. Increase Close Caution: Potentially contaminated area. Decontaminate the unit before the cover be opened. 24

30 l signal V- M1 PWM UP V- DWN P P V- 12V N UV Timer Unit UV Timer PWM -HI PWM -LOW tens HR. Min. units Adjust PWM-HI tens and units Fig. 6.1 POWER 25A UV 0.4A OUT LE L1 N Cover POWER 230 or 115VAC Fig. 6.2 Open the cover and loosen the M6 nut inside, then you can move the nut up and down to adjust the opening size of exhaust damper. 25

31 6.2 Inflow Velocity Measurement The range of inflow velocity for STERIZONE MSC is 0.51~0.56 m/s. There are two methods to determine the velocity. Primary Method - Direct Inflow Measurement (refer to NSF/ANSI , Annex A) 1. Open the window to the operation height (20 cm or 25 cm). 2. Place the capture hood at the center of the front opening of the cabinet. Seal the open areas on either side of the capture hood if necessary. 3. Take at least five readings and average them to determine the inflow volume rate. 4. Calculate the average inflow velocity in m/s by dividing the average inflow volume rate in m 3 /s by the front operation opening area in m 2 (W122 H20 cm or W122 H25 cm). Secondary Method - Thermal Anemometer (constricted access opening) If the primary method can t be performed, apply the secondary method to determine the inflow velocity. 1. Open the window to 7.62 cm (3 inch). 2. Place the probe in the vertical plane of the window, 3.81 cm (1.5 inch) down from the bottom edge of the window on a grid with end points cm (4.5 inch) in from the sides of the access opening and successive points spaced cm (4.0 inch) apart thereafter. 3. Eleven point readings are taken. Average the readings to determine the inflow velocity in m/s. 4. Multiply the average by the area of constricted opening, then divided by the area of operation opening (20 cm or 25 cm). 5. Multiply the calculating velocity by correction factor for STERIZONE series cabinets to obtain the average inflow velocity. 6.3 Fluorescent Lamp Replacement NOTE: Avoid Electrical Shock. Always unplug the power cable when performing any electrical maintenance or servicing. The procedures are as follows: 1. Unplug the cabinet to disconnect it from electrical power. 2. Loosen the screws securing the frontal plate and control plate to the cabinet showed as Fig. 6.3, then lift the frontal plate up to remove it and open the control plate. The 26

32 fluorescent lamp is located in the back of the control plate as Fig Rotate the fluorescent lamp and pull it straight out of its mounting sockets. 4. Remove the fluorescent lamp. 5. Place a new fluorescent lamp straight into the mounting sockets and rotate it until it locks into place. screws Fig. 6.3 Fluorescent lamp Fig

33 6.4 UV Lamp Replacement NOTE: The interior of the cabinet must be thoroughly disinfected before replacing the UV lamp. NOTE: Avoid Electrical Shock. Always unplug the power cable when performing any electrical maintenance or servicing. The procedures are as follows: 1. Wipe up the surface of UV lamp with 70% alcohol before replacing it. 2. Unplug the cabinet to disconnect it from electrical power. 3. Rotate the UV lamp and pull it straight out of its mounting sockets. 4. Remove the UV lamp. 5. Place a new UV lamp straight into the mounting sockets and rotate it until it locks into place. 6.5 HEPA Filters Replacement Caution: Potentially contaminated area. Decontaminate the unit before the seal cover be opened. A recertification must be done after changing filters. HEPA filters are designed to accumulate airborne particulates that include aerosol from the work area and the incoming air supply. It is no filtering function for the poisonous gas, volatile chemicals and radioactive substances. So the suitable preventive protective devices and methods of evaluation are important. The amount of accumulation is dependent on the amount of cabinet usage, the cleanliness of the room air and the amount of particulates generated during the procedures performed in the cabinet. As the filters accumulate particulates, an increase in pressure is required to pass air through the filters. When the negative pressure on gauge is less than 8.3 mmaq, the cabinet should be recertified and new filters installed. In general normal use condition, it can persist at least for 2~3 years. HEPA filters are extremely fragile and should not be 28

34 touched. Do not attempt to clean the filters in any way. To do so will damage the filters. Do not operate the cabinet if you suspect that the filter has been damaged. In specific circumstances need to replace the HEPA filter as follows: a. HEPA leakage has been found. b. Air negative pressure reading on gauge is less than 8.3 mmaq. c. The airflow has been adjusted by following section 6.1and the inflow rate still reduces more than 20%. d. The airflow has been adjusted by following section 6.1and the downflow rate still reduces more than 20%. NOTE: The replacement of HEPA filter must be operated by well trained and qualified personnel. Fumigation decontamination steps must be done and make sure disinfection completed before replacing the HEPA filter. The supply and exhaust HEPA filters are installed and removed through the front of the unit. The replacement procedures for HEPA filters are as follows: 1. Lower the window to the fully closed position. 2. Loosen the screws securing the frontal plate and control plate to the cabinet showed as Fig Lift the frontal plate up to remove it and open the control plate. 3. Remove the seal cover by loosening each hex flange nut on the cover as Fig Now, the filters are exposed as Fig Seal cover Frontal plate Fig

35 Blower Exhaust HEPA filter Supply HEPA filter Fig. 6.6 NOTE: Be careful! Don t damage the gaskets on the back of the cover. 4. Pull the compression rod down to release the filter as Fig Pull the compression rod down to release the supply HEPA filter. Fig

36 Push the compression rod up to fix the supply HEPA filter. Fig. 6.8 Exhaust HEPA Filter: Operation as the supply HEPA filter. Fig Remove the filter by sliding it straight out of the channel carefully. 6. Insert a new filter all the way into the channel carefully and push the compression rod up to fix the filter as Fig Reinstall the seal cover by tightening each hex flange nut. Avoid over tightening and don t use an impact driver for this procedure. IMPORTANT: Once the filters being removed from the cabinet, they should be put into a specific bag and disposed conforming to the local and national regulations and policies. 31

37 6.6 Blower Replacement Caution: Potentially contaminated area. Decontaminate the unit before the seal cover be opened. A recertification must be done after changing the blower. NOTE: Avoid Electrical Shock. Always unplug the power cable when performing any electrical maintenance or servicing. NOTE: Wait for at least 5 minutes for electricity release for the motor after disconnecting the power. The procedures are as follows: 1. Lower the window to the fully closed position. 2. Remove the frontal plate and open the control plate. 3. Remove the seal cover by loosening each hex flange nut on the cover as Fig Now, the blower is exposed. NOTE: Be careful! Don t damage the gaskets on the back of the cover. 4. Disconnect the power (5-pin) and control (4-pin) terminals for the motor as Fig Loosen each of the four screws securing the blower assembly to the cabinet, located on the four corners of the blower exhaust vent as Fig Now, the blower can be removed. 7. Install a new blower by reversing the above procedures. 32

38 5-pin connection 4-pin connection Fig Screws Fig The blower assembly is heavy. Please be careful to handle! 33

39 6.7 Maintenance Schedule Routine maintenance is important and necessary to keep optimum performance on the cabinet. The table below lists the maintenance schedule recommended. The interval may be greater or lesser depending on cabinet use. Interval Weekly Monthly Yearly Details Use 70% ethanol or other appropriate disinfectant to disinfect the inside of the cabinet and work surface. Use glass cleaner to clean the sash and the surface of UV lamp. Turn the blower on and note the pressure on the gauge. Use a damp cloth to clean the exterior surface of the cabinet. Lift the work surface, use 70% ethanol or other appropriate disinfectant to disinfect the lower plenum. Ensure that all service valves operate properly, and remove the right side plate to check the service lines. Perform all of the weekly maintenance operations listed above. Recertify the cabinet by a qualified certifier. Replace the UV lamp and/or fluorescent lamp. The replacement frequency is dependent on use. Perform all of the monthly maintenance operations listed above. 34

40 6.8 Labels Carefully note and observe the following safety labels when receiving, inspecting, installing, operating, maintaining and troubleshooting. Check the condition of the labels and replace if necessary. No Label Description Illustration Order Code 1 Exhaust damper warning The Exhaust Damper will effect inflow velocity, this procedure should be performed by qualified technicians only. A010 A010 2 Seal cover warning Potentially contaminated area. Prior to any disassembly for disposal, the cabinet must be decontaminated. A011 A011 3 Electrical hazard A102 UV Light Hazard. 4 UV light warning UV light can cause temporary or permanent loss of vision, and sunburn. A023 A103 35

41 5 Drain valve DRAIN VALVE A012 CLOSE before working. A012 6 Grounding A101 7 Leak test port warning Decontaminate cabinet before opening. A013 A013 8 Airflow sensor AIRFLOW SENSOR Do not obstruct space around se nsor. A014 A014 9 Model, serial no. and electrical power information A Motor speed adjuster Motor ADJUSTER This adjuster should be performed only by qualified personnel. A016 A Sash height Sash Height A017 A017 36

42 12 Service valve A Service lines SERVICE VALVE Do not use open flames. All gases MAX. pressure ar e 30PSIG. A018 A Biohazard warning A032 ELECTRICAL BOX 15 Electrical box Electric service should be performed only by qualified personnel. A019 A A Fuse 5A for outlets A020 10A for system 17 10A Fuse or 5A Fuse A021 5A for system 18 Outlet warning A022 MAX. TOTAL OUTLET LOAD 5A 37

43 VII. Trouble Shooting Follow to this manual suggested operation and maintenance procedures will make your MSC in the best usage proficiency and condition. It is strongly recommended that if the trouble shooting can do nothing with your MSC problem. Please don t open the MSC by yourself. Contact with your dealer or us from the contact information in this instruction manual. Problem Possible Causes Solution Cabinet doesn t work The power key isn t turned on Cabinet not plugged into socket Power failure Blown fuse Fan doesn t work Window is fully closed Solid state relay is defective Motor is defective Blown fuse Excessive fan noise HEPA filters overloading Foreign matter sucked into motor Light doesn t work Ballast is defective Lamp is defective Window is fully closed Limit switch is defective Blown fuse UV light doesn t work Ballast is defective Lamp is defective Window is opened Limit switch is defective Blown fuse Outlets don t work Outlet switch is off Overloading (5A). Blown Fuse Alarm doesn t work Mute switch is on Buzzer is defective Window height Limit switch is defective sensor doesn t work Contaminated work Leakage of HEPA filters Abnormal air flow Turn on the key. Plug the cabinet into socket properly. Check the power supply at the wall socket. Replace fuse. Open the window. Inspect solid state relay and replace it if necessary. Inspect motor and replace it if necessary. Replace Fuse Replace filters. Inspect motor and take out foreign matter. Inspect ballast and replace it if necessary. Inspect lamp and replace it if necessary. Open the sash. Adjust the switch or replace it if necessary. Replace fuse. Inspect ballast and replace it if necessary. Inspect lamp and replace it if necessary. Close the sash. Adjust the switch or replace it if necessary. Replace fuse Turn on the switch. Replace fuse. Check mute switch. Inspect buzzer and replace it if necessary. Inspect the switch and replace it if necessary. Inspect filters and have cabinet recertified. Have cabinet recertified. 38

44 VIII. Warranty Your STERIZONE Biosafty Cabinet is expressly warranted for one (1) year from date of installation to be free from manufacturing defects. Consumable parts, filters, lamps, glass and gaskets are excluded from this warranty. The manufacturer's exclusive obligation under this warranty shall be to supply, without charge, a replacement for any covered component of the Biosafty Cabinet which is found to be defective within one (1) year period. Defective parts must be returned no later than thirty (30) days after the failure by you to the installing contractor together with the model number, serial number, and installation date of the Biosafty Cabinet. You must pay shipping charges and all other costs of warranty service. CHUNG FU will not pay labor involved in diagnostic calls or in removing, servicing or replacing parts. This warranty shall not obligate the manufacturer for any labor costs and shall not apply to defects in workmanship or materials furnished by the installing contractor as contrasted to defects in the Biosafty Cabinet itself. Accident, shipping damage, misuse, improper installation and unauthorized modification, improper voltage, acts of God are also not covered. IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL BE LIMITED IN DURATION TO THE AFORESAID ONE (1) YEAR PERIOD. THE MANUFACTURER'S LIABILITY FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES, OTHER THAN DAMAGES FOR PERSONAL INJURIES, RESULTING FROM ANY BRANCH OF THE AFORESAID IMPLIED WARRANTIES OR THE ABOVE LIMITED WARRANTY IS EXPRESSLY EXCLUDED. CHUNG FU reserves the right to change the information in this manual without prior notice. 39

45 MSC Repair / Maintenance Records Install Date: Type: Location : S/N : Custodian : Date Repair/Maintenance Projects Service/Technician User/Custodian 40