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1 This annex is valid from: to Replaces annex dated: Industrieweg V ijhe Location(s) where activities are performed under accreditation ead Office Location Abbreviation/ location code odlocatie Industrieweg V ijhe Boseind RM Boxtel Roggedijk R elmond Den Sliem J Groenlo Grote tocht CG aandam B G This annex has been approved by the Board the Dutch Accreditation Council, on its behalf, J.A..M. de aas Director Operations Dutch Accreditation Council RvA Page 1 6
2 Type activity Internal reference number Location Sampling a. Meat, meat surfaces, deep muscle, carcasses Sampling for microbiological determinations PRO/MON/03 (carcasses in accordance with NEN-ISO 17604) b. Surfaces Sampling surfaces with swab or contact plate for microbiological determinations c. Process water Sampling for microbiological determinations Chemical PRO/MON/04 in accordance with NEN-ISO PRO/MON/01 1 ater Determination p levels; potentiometry CL/O/23 2 ater Determination electrical conductivity; conductometry Microbiological CL/O/02 in accordance with NEN-ISO Manure and meat from poultry Manure and digestate Detection Salmonella; qualitative analysis, MSRV ML/PL/01 in accordance with NEN-EN-ISO 6579: amd.1 Annex D Manure and digestate: 4 Foodstuffs and feeds Enumeration lactic acid bacteria at 30 C; pour plate technique ML/AL/13 in accordance with NEN-ISO Foodstuffs Detection Listeria monocytogenes; qualitative analysis, Rapid L.mono 6 Enumeration Listeria monocytogenes; streak plate technique 7 Detection Escherichia coli; qualitative analysis 8 Screening for stx genes (STEC), qualitative analysis, PALL genedisc ML/AL/15 equivalent to NEN-EN-ISO (AFNOR BRD 07/04-09/98) ML/AL/16 equivalent to NEN-EN-ISO ML/AL/18 ML/AL/53 Dutch Accreditation Council RvA Page 2 6
3 Type activity Internal reference number Location 9 Manure, digestate and compost Enumeration Enterococci ML/AL/45 10 Foodstuffs, feeds, manure Determination total aerobic colony numbers at 30 C; ML/AL/42 equivalent to NEN-EN-ISO 4833 (AFNOR 3M 01/01-09/89) Feeds and manure: 11 Foodstuffs, feeds, manure, compost Enumeration ß D glucoronidase positive Escherichia coli at 42 C; ML/AL/41 equivalent to NEN-ISO (AFNOR 3M 01/08-06/01) Feeds, manure and compost: in house method 12 Foodstuffs and feeds Enumeration coliforms at 37 C; ML/AL/54 equivalent to NEN-ISO 4832 (AFNOR 3M 01/02-09/89) Feeds: 13 Foodstuffs, feeds, manure, compost Enumeration Enterobacteriaceae at 37 C; ML/AL/55 equivalent to NEN-ISO (AFNOR 3M 01/06-09/97) Manure and compost: 14 Foodstuffs and petfoods Enumeration coagulase positive Staphylococci at 37 C; counting plate, petrifilm ML/AL/60 equivalent to NEN-EN-ISO (AFNOR 3M 01/09-04/03A) 15 Foodstuffs, feeds, compost Detection Salmonella; qualitative analysis, MDS ML/AL/61 equivalent to NEN-EN-ISO 6579 (AFNOR 3M 01/11-11/12) Feeds and compost: 16 Foodstuffs and feeds Enumeration yeasts and moulds, petrifilm, RYM ML/AL/62 equivalent to NEN-ISO and NEN-ISO (AFNOR 3M 01/13-07/14) 17 Swimming pool water Enumeration spores Sulfite reducing clostridia; membrane filtration ML/O/05 in accordance with NEN-ISO Surface water Enumeration thermotolerant coliforms; membrane filtration ML/O/07 equivalent to NEN 6570:1982 Dutch Accreditation Council RvA Page 3 6
4 Type activity Internal reference number Location 19 Drinking water, process water, surface water, water and groundwater Enumeration intestinal Enterococci at 36 C; membrane filtration ML/O/08 in accordance with NEN-EN-ISO Drinking water, water, water from cooling towers, process water and wastewater Enumeration colonyforming units Legionella bacteria; membrane filtration, streak plate technique ML/O/09 in accordance with NEN Drinking water, water, water from cooling towers, process water and wastewater Enumeration colonyforming units Legionella bacteria; membrane filtration, confirmation with real-time PCR ML/O/09 analysis in accordance with NEN 6265; confirmation equivalent to NEN Legionella isolates Confirmation Legionella pneumophila suspected colony s; real-time PCR 23 Serotyping legionella pneumophila; latex agglutination test 24 Drinking water, water, groundwater and process water Determination aerobic colony numbers at 22 C en 36 C; pour plate technique 25 Enumeration Escherichia coli and coliforms; membrane filtration ML/O/12 ML/O/12 ML/O/10 in accordance with NEN-EN-ISO 6222 ML/O/11 in accordance with NEN-EN-ISO Drinking water, surface water, water and process water Enumeration Pseudomonas aeruginosa at 36 C; membrane filtration ML/O/14 in accordance with NEN-EN-ISO Drinking water and process water Enumeration Aeromonas at 30 C; membrane filtration ML/O/13 in accordance with NEN Swimming pool water Enumeration coagulase positive Staphylococci in water, membrane filtration ML/O/15 (isolation medium en confirmation in accordance with NEN-EN- ISO ) Dutch Accreditation Council RvA Page 4 6
5 Type activity Internal reference number Location 29 Meat and meat replacing s Enumeration ß D glucoronidase positive Escherichia coli at 42 C; ML/AL/41 equivalent to NEN-ISO (AFNOR 3M 01/08-06/01) 30 Determination total aerobic colony numbers at 30 C; ML/AL/42 equivalent to NEN-EN-ISO 4833 (AFNOR 3M 01/1-09/89) 31 Detection Salmonella; qualitative analysis, MDS 32 Detection Escherichia coli O157 including 7; qualitative analysis, MDS 33 Meat Enumeration Enterobacteriaceae at 37 C; ML/AL/61 equivalent to NEN-EN-ISO 6579 (AFNOR 3M 01/11-11/12) ML/AL/63 equivalent to NEN-EN-ISO (AFNOR 3M 01/12-03/13) ML/AL/55 equivalent to NEN-ISO (AFNOR 3M 01/06-09/97) 34 Meat, meat surfaces Determination total aerobic colony numbers at 30 C; ML/AL/42 equivalent to NEN-EN-ISO 4833:2003 (AFNOR 3M 01/01-09/89) 35 Enumeration coliforms at 37 C; 36 Enumeration Enterobacteriaceae at 37 C; 37 Meat Enumeration ß D glucoronidase positive Escherichia coli at 42 C; 38 Enumeration coagulase positive Staphylococci at 37 C; counting plate, petrifilm ML/AL/54 equivalent to NEN-ISO 4832 (AFNOR 3M 01/02-09/89) ML/AL/55 equivalent to NEN-ISO (AFNOR 3M 01/06-09/97) ML/AL/41 equivalent to NEN-ISO (AFNOR 3M 01/08-06/01) ML/AL/60 equivalent to NEN-EN-ISO (AFNOR 3M 01/09-04/03A) 39 Meat, environmental samples Detection Listeria monocytogenes; qualitative analysis, Rapid L.mono ML/AL/15 equivalent to NEN-EN-ISO (AFNOR BRD 07/04-09/98) 40 Meat, meat surfaces Detection Salmonella; qualitative analysis, MDS ML/AL/61 equivalent to NEN-EN-ISO 6579 (AFNOR 3M 01/11-11/12) Dutch Accreditation Council RvA Page 5 6
6 Type activity Internal reference number Location Trichine 41 Meat Detection Trichine larvae with collecting digestion method TRI/VL/01 in accordance with EU 2015/1375 B,, G Dutch Accreditation Council RvA Page 6 6
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