Title: Managing Foliar Feeding Beetles Protocol #:

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1 Protocol #: p 1 of 6 Objective: Determine efficacy of various products against foliar feeding beetles on ornamental horticulture plants. Experimental Design: Plot Size: Must be adequate to reflect actual use conditions. Replicates: Minimum of 6 replications. Target Species: Flea beetles, Viburnum leaf beetle, Adult Japanese Beetles. Contact your regional coordinator if other target species are of interest. Plant Hosts: Use a plant host suitable for target species, recording species and variety used. Record crop species and variety used. Treatments: See treatment table on next page. Test materials should have been stored for no more than 2 years prior to start of experiment. Biological test materials should be requested for arrival approximately 2 weeks prior to first application unless otherwise directed by manufacturer. Application Instructions: Apply each treatment according to directions in the table below. All treatments are foliar applications. Applications should be made using application equipment consistent with conventional commercial equipment. Calibrate application equipment prior to application. Read available labels before making applications. Avoid treating plants under unusually extreme environmental conditions. Use Site: May be greenhouse, field container, or field in-ground. May be caged or open trials. Evaluations: Evaluate mortality (counts of live/dead adult beetles) or abundance and feeding damage (% herbivory on certain number of leaves of a similar age or % defoliation; note age of foliage). Evaluate prior to application and then 3, 7, 14, and 28 days (optional). Record plant height & width at initial and final evaluations only. Record phytotoxicity at each rating date on a scale of 0 to 10 (0 = no phytotoxicity; 10 = complete kill). If phytotoxicity is observed in treated plants, take pictures comparing treated and untreated plant material. Maintenance Applications: Applications during an experiment to manage non target diseases, pests or weeds may be needed to appropriately evaluate efficacy or crop safety. Consult Appendix 1 for known negative impacts due to simultaneous or sequential applications of certain products with test materials If applications must be made of any products in Appendix 1, please contact IR-4 prior to application. Recordkeeping: All operations, data and observations appropriate to this study should be recorded. It is helpful to review the Ornamental Horticulture Research Report Form in advance. Keep detailed records of weather conditions including temperature, precipitation and/or irrigation (please note whether irrigation is sub-surface or overhead), and relative humidity with a minimum of high, low and average daily temperatures. Other information to record includes soil-type or soil-less media, application equipment, irrigation (type & frequency), liner size, plant height & width, and plant growth stage at application and data collection dates. Content of reports should be sufficient to fully understand how the experiment was conducted. Photographs often illustrate experimental design, site conditions, sampling methods, and impacts of treatments very well. It is encouraged to include a picture or two of the greenhouse, field or landscape where the experiment is sited. It is highly encouraged that pictures illustrating treatment effects are taken if and when these impacts are visually apparent. If different application methods or evaluations are made, please clearly specify differences in final report and explain reason for change.

2 Protocol #: p 2 of 6 Treatments: Treatment number Applications Evaluations for specific beetles Rate (per 100 Week Days after treatment Priority Flea Jap. Other Product gal) * fl oz X E E E E Hachi Hachi SC (tolfenpyrad) fl oz X E E E E fl oz X X* E E E E IKI-3106 (cyclaniliprole) A fl oz X X* E E E E VST % LI liter X X X E E E E 4 4 VST BeetleGone! + 0.1% LI liter + 16 lb X X X E E E E 5 5 BeetleGone! + Complex surfactant 16 lb X X X E E E AzaGuard 16 fl oz X X X E E E E lb X X X E E E BeetleGone! + NuFilm 17 Optional 8 lb X X X E E E BotaniGard ES 32 fl oz X X X E E E Lynx EC 5.0 (pyrethrins) 16 oz X X E E E E A/C MBI-203 WDG (Chromobacterium subtsugae) + NuFilm 17 3 lb X X X E E E 6-9 Preferal (Isaria fumosorosea Apopka Strain 97) 1 lb X E E E a Venerate XC (Burkholderia rinojensis strain A396) + NuFilm 17 1 gallon X X X E E E - 10b Xpectro OD (pyrethrins + Beauveria bassiana Strain GHA) 32 oz X X E E E B XXpire (spinetoram + sulfoxaflor) 3.5 oz + Capsil at 6 oz X X E E E E acephate X X E E E bifenthrin X X E E E carbaryl See label X X E E E Standards (Pick One) imidacloprid directions X X E E E permethrin X X E E E Scimitar (lambda-cyhalothrin) X X E E E TriStar (acetamiprid) X X E E E Untreated E E E * If a second application is needed, apply 2 weeks after initial treatment. Special Instructions: NuFilm 17 should be applied with BeetleGONE!, MBI-203 WDG, and Venerate XC at a rate of 0.125% v/v BotaniGard ES should not be applied through a thermal pulse fogger VST spray to wet only. Do not spray to drip. Include the surfactant LI700. For the tank mix with BeetleGone! replace NuFilm with LI700

3 Notations on Treatment List: AzaGuard: A for Viburnum leaf beetle only BeetleGONE!: already registered for Japanese beetles. BotaniGard ES: A for Japanese beetle adults and Viburnum leaf beetle only Lynx EC 5.0: already registered for elm leaf beetle, flea beetles and Japanese beetles, A for viburnum leaf beetle only MBI-203 WDG: Viburnum leaf beetle only. Xpectro OD: already registered for elm leaf beetle, flea beetle. A for Japanese beetles and viburnum leaf beetle Venerate XC: Viburnum leaf beetle only. XXpire: flea beetle only Sources for Products: p 3 of 6 When placing requests for samples, please ensure you include the amount needed, the first planned application date, and shipping address and phone number. Product Contact Phone AzaGuard BioSafe Systems, Vijay Choppakatla x 225 vijayc@biosafesystems.com BeetleGONE! Phyllom BioProducts, Kurt Schwartau kurtschwartau@phyllom.com BotaniGard ES BioWorks, Dan Peck x337 dpeck@bioworksinc.com Complex surfactant Vestaron, Don Lester dlester@vestaron.com Hachi-Hachi SePro, Kyle Briscoe kyleb@sepro.com IKI-3106 ISK, Sean Whipple whipples@iskbc.com Lynx EC 5.0 LAM International, Luis Mazariegos lamh@lamintl.com MBI-203 Marrone Bio Innovations, Tim Johnson tjohnson@marronebio.com Preferal SePro, Kyle Briscoe kyleb@sepro.com Venerate Marrone Bio Innovations, Tim Johnson tjohnson@marronebio.com VST Vestaron, Don Lester dlester@vestaron.com Xpectro LAM International, Luis Mazariegos lamh@lamintl.com XXpire Dow AgroSciences, Jamie Breuninger jbreuninger@dow.com Reports: Reports are due 60 days after last data collection date. Submit reports electronically on the standard IR-4 Ornamental Horticulture Research Report Form. The basic report can be sent in MS Word, the recordkeeping information as pdf or other electronic documents, the raw data in MS Excel or other suitable program such as ARM, and photographs can be submitted as picture embedded in the report or as separate jpg, bmp, or tiff files. Please direct questions to: Cristi Palmer, IR-4 HQ, Rutgers University, 500 College Road East, Suite 201W, Princeton, NJ 08540, Phone x4629, palmer@njaes.rutgers.edu. OR Ely Vea, 308 Aston Forest Lane, Crownsville, MD 21032, Phone & FAX#: , evvea@njaes.rutgers.edu. Draft Date: 7/11/2017 Revised By: EV & CLP

4 APPENDIX 1 Title: Managing Foliar Feeding Beetles p 4 of 6 Greenhouse Insecticides and Miticides: Plant Sensitivity Dan Gilrein Extension Entomologist Cornell Cooperative Extension of Suffolk County Adept: Do not apply to poinsettias, hibiscus, or Rieger begonia. Aria: Some sensitivity seen on pansy (and viola) cultivars Avid/generics: Not recommended for use on ferns or Shasta daisy. We have seen very slight bronzing on white bracts of some poinsettias but only at the 8 oz rate. Should not be used for suppression of aphids, whiteflies, and thrips on roses, chrysanthemums, and gerbera since these are hosts for primary pests (mites and leafminers) targeted by Avid. BotaniGard ES: We have observed injury to poinsettia and to tomato with the liquid but not with the WP formulation. Conserve SC: Label warns some spotting of Saintpaulia (African violet) flowers may occur. Distance IGR: Phytotoxicity has been observed on the following plants: Salvia (Salvia spp.), Ghost Plant (Graptopetalum paraguayense), Boston Fern (Nephrolepis exaltata), Schefflera (Schefflera spp.), Gardenia (Gardenia spp.) and Coral Bells (Heuchera sanguinea). It is therefore recommended that DISTANCE not be used on these plants. Do not apply to poinsettia after bract formation. DuraGuard ME: Direct treatment to some open blooms may cause petal drop. Do not use on kalanchoes. Endeavor: Do not apply to poinsettias after bract formation. Hachi-Hachi: NOT FOR USE IN NEW YORK STATE Do not apply to Salvia spp., Impatiens spp, Gypsophila spp, or poinsettias with bracts in color. Temporary phytotoxicity has been seen on Colocasia, Geranium, Lobelia, Pansy (flowers), Verbena, and Vinca. We noted some injury to Intensia Blueberry phlox but not other varieties in one trial. Hexygon DF: On orchids, do not tank mix with Plyac or household detergents. Do not apply to plants under stress or those not acclimated to a new environment. Horticultural oil (SuffOil-X, SunSpray Ultra-Fine Spray Oil, Ultra-Pure Oil): Note tank mix incompatibilities and restrictions or cautions concerning application to some woody plants. Do not use around time of chlorothalonil (Daconil) application, or in combination with sulfur or some other fungicides. We have seen some sensitivity on some greenhouse crops so be sure to test first. Do not apply to plants under moisture or drought stress and when environmental conditions are unfavorable (high heat and humidity).

5 p 5 of 6 Judo: Do not use Judo on geraniums (Pelargonium sp.), Peperomia, Dracaena, and 'Classy', 'Attache', or 'Vogue' varieties of rose. Insufficient information on tolerance is available for lily-of-the-incas (Alstromoeria), New Guinea impatiens, Bacopa, ferns, phlox, English ivy (Hedera helix), cyclamen, Agyranthemum, hydrangea, schefflera, Matthiola (stock), Mexican heather, Lobelia, fuchsia, Cordyline, croton, neanthebella palm, and primula. It is recommended that Judo not be used on these plants. Do not apply more than 2 fl. oz. per 100 gal per application or 4 fl. oz. per cropping cycle to chrysanthemum, Shasta daisy, snapdragon, impatiens, verbena, lantana, gerbera daisy, or marigolds. Kontos: not recommended for: geraniums (Pelargonium spp.), orchids, hoya, Dracaena, Cordyline, schefflera, neanthebella palm, and ferns. Do not make more than one application per season to Hydrangea, Impatiens spp., crotons (Codieum spp.), fuchsia hybrids, petunia, peperomia, stock, or cyclamen. IR-4 studies ( found slight height reduction, leaf curling, bleaching of flowers or plant death in Begonia sp, Coleus x hybridus, Petunia sp., Pelargonium sp., Vinca sp., and Viola sp.) at the 2X and 4X rates applied as drench. Verbena x hybrida exhibited significant flower discoloration at all rates applied as drench in one trial. The study findings recommend adding label prohibition against drench application on Begonia sp., Coleus x hybridus, Petunia sp., Pelargonium sp., Verbena x hybrida, Vinca sp., and Viola sp. but note these treatments were at twice and four times label rate, and labels already advise against application to Pelargonium and limit to one application to petunia. M-Pede: Note label discussion of plant sensitivity, tank mix incompatibilities, and ph. Do not apply to horsechestnut, Japanese maple, mountain ash, Cherimoya, bleeding heart, or sweetpeas. Bald cypress, begonia, chrysanthemum, Crown of Thorns and other euphorbia, cucumber, delicate ferns, narrow leaf evergreens (especially when stressed or when tender new growth is present), Dieffenbachia, fuchsia, gardenia, impatiens, jade plant, lantana, ornamental ivy, palms, poinsettia, redbud, river birch, schefflera, Zebra plant and some succulents may be sensitive. Flowers of plants including African violet, ageratum, azalea, begonia, camellia, chrysanthemum, dahlia, geranium, gloxinia, impatiens, lily, marigold, orchid, pansy, petunia, poinsettia bracts, rose, salvia, snapdragon, vinca and zinnia may be injured when sprayed. Magus/ Magister: Do not apply to roses or to plants under stress. Orthene TTO/Acephate/Avatar/Lepitect: Phytotoxicity has occurred on the following foliage plants: Bletchum gibbum, Cissus antarctica, Ficus triangularis, Fittonia verchaffeltii, Maranta leuconeura kerchoveana, Pachystachya lutea, Plectranthus australis, Polypodium aureus, Polystichum, Pteris ensiformis, Tolmiea menziesii. Before treating large plantings spray only a few plants and observe 2 weeks for varietal phytotoxicity. Application on poinsettias after bract formation may result in phytotoxicity on certain varieties. Application to roses in flower may result in flower damage. Do not apply more often than once every 28 days to carnations and chrysanthemums. Phytotoxicity has occurred on the following chrysanthemum varieties: Albatross, Bonnie Jean, Dixie, Garland, Gem, Iceberg, Pride, Showoff, Statesman, Tally Ho, Westward Ho and Wild Honey. Application to chrysanthemums in flower may result in flower damage. Ovation: Bright magenta residue may be apparent on light-colored flowers and foliage. Pedestal: Do not apply to poinsettias.

6 p 6 of 6 ProMITE/Vendex: Under greenhouse conditions, foliage and flowers of certain species may demonstrate sensitivity to repeat applications. Occasional minor sensitivity has been observed on Salix melanostachys (common willow), Asplenium bulbiferum (spleenwort garden fern), Dryopteris erythrosora (wood fern), Cercis canadensis (common redbud), Camellia japonica (red garden camellia), Pellaea rotundifolia (button fern), Davallia fejeensis (rabbit foot fern), Asparagus meyeri (Meyers asparagus fern), Asplenium nidus (birdnest fern), Adiantum cuneatum (maidenhair fern), Celosia argentea (cockscomb), Verbenea hortensis (verbena), Ageratum houstonianum (floss flower), and Rosa sp. (common rose some varieties, esp. yellow). Pylon: Phytotoxicity is likely following application to Dianthus (including carnations, pinks and Sweet William varieties, Dianthus spp.), Kalanchoe (Kalanchoe blossfeldiana), poinsettia (Euphorbia pulcherrima), roses (Rosa spp.), Salvia (Salvia spp.) Zinnia (Zinnia spp.). Avoid application to plugs in early development (1 2 leaf stage). Tank mixing with crop oils, surfactants and fertilizer adjuvants is not recommended. Shuttle-O: Test first on impatiens and roses before large-scale use. Tame 2.4EC: High-gallonage applications to certain chrysanthemum varieties may result in crop injury. Do not apply to chrysanthemums and roses with open flowers. Application of Tame 2.4EC + Orthene tank mix on poinsettias after bract formation may result in phytotoxicity on certain varieties. Tetrasan 5WDG: Do not apply to poinsettia after bract formation Triact 70: Do not apply to known sensitive plant species, such as impatiens flowers, fuchsia flowers, hibiscus flowers, some rose flowers, ornamental olive trees or some carnations varieties, without prior testing. Do not apply to wilted or otherwise stressed plants, or to newly transplanted materials prior to root establishment. Use with care on plants with tender tissue. TriStar 8.5SL: There have been recent reports of some injury to certain verbena cultivars (Aztec series). Flowers don t develop normally or fail to open. The effect may be temporary, but test first before large-scale use 10/21/2014

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