Title: Efficacy of Management Tools for Armored Scale in Ground Protocol #:

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1 Protocol #: p 1 of 5 Objective: Determine efficacy of various products against armored scale on ornamental plants in ground. Do not conduct in containerized ornamentals. Experimental Design: Plot Size: Must be adequate to reflect actual use conditions. Replicates: Minimum of 6 replications. Target Species: Camellia, Euonymus, Japanese Maple, and Tea scales. Contact your regional coordinator if other target species are of interest. Plant Hosts: Use a plant host suitable for target scale species, recording species and variety used. Record crop species and variety used. Treatments: See treatment table on next page. Test materials should have been stored for no more than 2 years prior to start of experiment. Biological test materials should be requested within 2 weeks prior to first application unless otherwise directed by manufacturer. Application Instructions: Apply each treatment according to directions in the table below. All treatments are foliar applications. Applications should be made using application equipment consistent with conventional commercial equipment. Calibrate application equipment prior to application. Read available labels before making applications. Avoid treating plants under unusually extreme environmental conditions. Use Site: Field In Ground. For greenhouse or field container, refer to protocol Evaluations: Record initial insect counts and then 7, 14 (prior to 2 nd application), 28 days, and six months after initial application or at an appropriate time point to assess efficacy. Record phytotoxicity at each rating date on a scale of 0 to 10 (0 = no phytotoxicity; 10 = complete kill). If phytotoxicity is observed in treated plants, take pictures comparing treated and untreated plant material. Maintenance Applications: Applications during an experiment to manage non target diseases, pests or weeds may be needed to appropriately evaluate efficacy or crop safety. Because of potential negative impacts due to simultaneous or sequential applications of certain products with test materials please avoid applying the following: If applications must be made of any products on the above list, please contact IR-4 prior to application. Recordkeeping: All operations, data and observations appropriate to this study should be recorded. It is helpful to review the Ornamental Horticulture Research Report Form in advance. Keep detailed records of weather conditions including temperature, precipitation and/or irrigation, and relative humidity with a minimum of high, low and average daily temperatures. Other information to record includes soil-type or soil-less media, application equipment, irrigation (type & frequency), liner size, plant height & width, and plant growth stage at application and data collection dates. Content of reports should be sufficient to fully understand how the experiment was conducted. Photographs often illustrate experimental design, site conditions, sampling methods, and impacts of treatments very well. It is encouraged to include a picture or two of the greenhouse, field or landscape where the experiment is sited. It is highly encouraged that pictures illustrating treatment effects are taken if and when these impacts are visually apparent. If different application methods or evaluations are made, please clearly specify differences in final report and explain reason for change.

2 Protocol #: p 2 of 5

3 p 3 of 5 Treatments: Priority # Product Rate Application Instructions 7 fl oz per 100 gal OR 1 BAS I 7 fl oz per 100 gal + UltraPure Oil* Full coverage spray at crawler stage and every 2 per 100 gal for Camellia and tea weeks for a maximum of 3 applications. scales A B BYI SL IKI-3106 (cyclaniliprole) Mainspring 200SC (cyantraniliprole) 2.7 fl oz per 100 gal (90 g ai per hectare; assuming 200 gal per acre) Full coverage spray at crawler stage and every 5.4 fl oz per 100 gal (180 g ai per week for a maximum of 3 applications. hectare; assuming 200 gal per acre) 22 fl oz per 100 gal + MSO or NIS at appropriate rate Full coverage spray at crawler stage and every 2 28 fl oz per 100 gal + MSO or NIS weeks for a maximum of 3 applications. at appropriate rate Shrubs: fl oz per foot of height Trees:0.125 fl oz per inch dbh Shrubs: 0.25 fl oz per foot of height Trees:0.25 fl oz per inch dbh A/N 8 Talus 70DF (buprofezin) 14 oz/100 gal Foliar application 20 gm/1 gal container Flagship G (thiamethoxam) 40 gm/2 gal container 60gm/3 gal container Flagship 25WG (thiamethoxam) Kontos (spirotetramat) Safari 20SG (dinotefuran) Two drench or surface applications. Apply within 1-3 feet of the root flare of trees and shrubs. Apply first application 30 days prior to infestation if test system allows. Apply second application 28 days after first. Single drench or surface application. Apply within 1-3 feet of the root flare of trees and shrubs. Apply first application 30 days prior to infestation if test system allows. Broadcast evenly around the surface of the growing media 0.5 g product/ ft of shrub height Apply in enough drench volume to wet the upper ½ to ¾ of the root zone. 3.4 oz per 100 gal Foliar 3.4 oz per 100 gal Drench Single soil drench: 4 fl oz of drench solution/ Container: 18 oz/ 100 gal gallon of potting media. Apply evenly to soil surface. Tank Mix: Distance + TriStar 12 fl oz + 12 fl oz per 100 gal Apply at crawler emergence. Xxpire 40WG (spinetoram oz per 100 gal+ Capsil at 6 oz sulfoxaflor) per 100 gal Protocol #:

4 p 4 of 5 Standards (Pick One) TriStar 8.5SL (acetamiprid) 3.5 oz per 100 gal+ Capsil at 6 oz per 100 gal 16.5 fl oz per 100 gal 9a Orthene TTO See label directions 9b Horticultural Oil UltraPure Oil See label directions 9c Acephate E 75 WP (acephate) 0.67 lbs/100 gal 9d Duraguard ME or Chlorpyrifos E-Pro 2 (chlorpyrifos) 2 qts/100 gal 9e Distance (pyriproxifen) 12 fl oz per 100 gal 10 Untreated Make applications at crawler stage on a 14 day schedule. Make no more than 2 consecutive applications. Spray to wet; two foliar sprays on a 14 d interval with a wetting agent such as Capsil. Full coverage spray at crawler stage and every 4 weeks for a maximum of 3 applications. Full coverage spray at crawler stage or early stages of settled scales. Two foliar sprays on a 21 day interval. Apply at crawler stage. Protocol #:

5 Protocol #: p 5 of 5 Sources for Products: Product Contact Phone BAS 440 BASF, Kathie Kalmowitz kathie.kalmowitz@basf.com BYI 2960 Bayer, Ken Kukorowski ken.kukorowski@bayer.com Distance NuFarm, Jill Calabro Jill.Calabro@us.nufarm.com Duraguard ME BASF, Kathie Kalmowitz kathie.kalmowitz@basf.com Flagship 25WG Syngenta, Steve Cosky steve.cosky@syngenta.com Flagship G Syngenta, Steve Cosky steve.cosky@syngenta.com IKI-3106 ISK, Sean Whipple whipples@iskbc.com Kontos OHP, Carlos Bogran cbogran@ohp.com Mainspring Syngenta, Steve Cosky steve.cosky@syngenta.com Safari 20SG NuFarm, Jill Calabro Jill.Calabro@us.nufarm.com Talus 70DF SePRO, Richard Dirks RichardD@sepro.com TriStar 8.5SL NuFarm, Jill Calabro Jill.Calabro@us.nufarm.com Ultra Pure Oil BASF, Kathie Kalmowitz kathie.kalmowitz@basf.com Xxpire 40WG Dow AgroSciences, Dan Loughner dloughner@dow.com Reports: Reports are due 60 days after last data collection date. Submit reports electronically on the standard IR-4 Ornamental Horticulture Research Report Form. The basic report can be sent in MS Word, the recordkeeping information as pdf or other electronic documents, the raw data in MS Excel or other suitable program such as ARM, and photographs can be submitted as picture embedded in the report or as separate jpg, bmp, or tiff files. Please direct questions to: Cristi Palmer, IR-4 HQ, Rutgers University, 500 College Road East, Suite 201W, Princeton, NJ 08540, Phone x4629, palmer@aesop.rutgers.edu OR Ely Vea, 308 Aston Forest Lane, Crownsville, MD 21032, Phone & FAX#: , evvea@comcast.net. Draft Date: 4/13/2015 Revised By: EV & CLP

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