KIVEX BIOTEC Ltd. Catalogue 2006/07

Transcription

1 KIVEX BIOTEC Ltd. Catalogue 2006/07

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3 Dear Customer, Since 1986 K-SYSTEMS has been manufacturing products for the IVF market World Wide. In 1999 K-SYSTEMS became part of the Kivex organisation and substantial capital was invested in developing a new range of products especially for the IVF market. K-SYSTEMS products have always been signified by high quality and qualified technical service, based on local support. Today K-SYSTEMS is represented by local distributors in 44 countries servicing and offering our wide range of products: IVF Workstations, Laminar Flowhoods, Biosafety Cabinets, Mini Incubators, Anti Vibrations Tables, Warming Plates, Dry Bath Incubators etc. Although the K-SYSTEMS Catalogue 2006/07 contains a wide variety of products, we are continuously working on new product developments and improvements of existing ones. This is why you should pay attention to our website to find the latest news. We welcome you as a customer and hope this catalogue will inspire you to buy our products. Best regards K-SYSTEMS, Kivex Biotec Ltd.

4 IVF Workstations 5 Standard features 18 Optional features 20 Laminar Flow Hoods 23 Accessories 26 Light Sources 28 Free Standing Warming Table 29 AntiVibration Tables 30 Gas Mixer 31 Warming Plates 32 Trolley with warmed table top 33 Gassing Humidifier 34 Incubators 35 Bench Top 35 Portable 36 DryBath 38 Large CO 2 39 Freeze Control Systems 39 Future Products 43 Index 45 All information is valid from June 1 st K-SYSTEMS reserves the right to make changes in specifications, materials, product range and prices without notice. Production: Creative Design Frontpage artwork: Christina LaForce - Technique: Watercolor The artwork can be purchased as a reproduction through K-SYSTEMS

5 ivf workstations & Laminar flowhoods K-SYSTEMS IVF Workstations The K-SYSTEMS Workstations are dedicated to IVF and the many ART techniques. The idea is to achieve the best possible environment for the gametes and embryos during the various procedures. This requires control of temperature and humidity in order to maintain correct ph-level. The K-SYSTEMS Workstations offer the possibility to control these parameters and in addition assure that everything takes place in an aseptic environment, created by a laminar flow with a noise level of only 51dB. The K-SYSTEMS IVF Workstations is offered in the three various series. 1) The 100 Workstation Series offers you state of the art technology and a wide range of options to respond to your specific IVF needs and techniques. 2) The 200 Workstation Series offers a biohazard functionality providing simultaneous product and operator protection. Elegant design combined with functionality, gives you an optimum solution. 3) The 400 Workstation is the entry range series. It accommodate the needs of basic IVF procedures in a lean cost structure, without compromising performance or the renowned K-SYSTEMS quality.

6 ivf workstations The 100 Series Workstation The 100 Series workstations offers a variety of models and configurations to fit your workstation to your needs. All workstations have a noise level on only 51 db and an integrated warmed area in the table top based on a stable controlled temperature system. Various heating patterns are available. The table table top is preparred for one or two stereomicroscopes and light source bases. Most stereomicroscopes can be incorporated. Additionally, all models have a gassing and humidification system integrated in the back wall with heated gas coming through two nozzles in the table top, making it possible to avoid large fluctuations in CO2 conditions. In addition this series offers a wide range of options which can be incorporated into the workstations (please refer to page 20). Available in 4, 5 and 6 foots lenghts (120, 150 and 180 cm). Two year warranty, includes parts and labor. K-SYSTEMS AluHeat Systems AluHeat was developed by K-systems and is a result of years of experience in manufacturing heat exchangers. AluHeat is an aluminium composite sandwich construction with an integrated warming element and temperature sensors. The warmed mass of aluminium gives off a solid distribution of warmth and secures an even and stable temperature of the table top, which is resistant to thermal shock. Thermal shock is caused by overshooting the temperature when, for example, a cold element is placed on the table top. This typically happens when using electrical heating systems. Advantages such as low maintenance, improved heating of the critical area around the light opening, and the absence of a noisy source of contamination i.,e. water bath, have proven to be invalauble in the more than 200 AluHeat system installed world wide.

7 ivf workstations Workstations prepared for one Stereo Microscope Model L124 IVF Designed as a single workplace and for the mounting of a stereomicroscope, right sided. For options available see page 20. L124 Overall dimensions (WxDxH) 1246x735x2015 mm/49x29x79 Workspace/tabletop (WxD) 1226x553 mm/40x18 Air flow 0,32 m/s Table height 773 mm Order Code Model L125 IVF Designed as a single workplace and for the mounting of a stereomicroscope, right sided. Compared to L124 this models offers more table space. For options available see page 20. Model L126 IVF Designed as a single workplace and for the mounting of a stereomicroscope, right sided. Offers adequate workspace for integration of more options like an incubator and a monitor in the back wall. For options available see page 20. L125 Overall dimensions (WxDxH) 1546x735x2015 mm/61x29x79 Workspace/tabletop (WxD) 1526x553 mm/50x18 Air flow 0,32 m/s Table height 773 mm Order Code L126 Overall dimensions (WxDxH) 1846x735x2015 mm/73x29x79 Workspace/tabletop (WxD) 1826x553 mm/59x18 Air flow 0,32 m/s Table height 773 mm Order Code 11165

8 ivf workstations Workstations prepared for two Stereomicroscopes L126 Dual IVF Designed as two workplaces and for mounting of two stereomicroscopes. A dual workstation offers the potential to simultaneously set up a perfect rotation system / organize division of work and keep the workflow of individuals undisturbed. E.g.: in the morning: one technician checks the oocytes for fertilization or scores embryos, and transfers them to new dishes. Meanwhile, another technician can prepare the dishes for cryopreservation and runs the cells / embryos to be cryopreserved through the series of cryoprotectants. L126 Dual Overall dimensions (WxDxH) 1846x735x2015 mm/73x29x79 Workspace/tabletop (WxD) 1826x553 mm/59x18 Air flow 0,32 m/s Table height 773 mm Order Code 11166

9 ivf workstations Workstations prepared for one inverted microscope and one Stereomicroscope L126 MP Designed as two workplaces the right side is for mounting of a stereomicroscope and left side is dedicated to an inverted microscope for performing ICSI in clean air. The MP version is cost and space saving by integrating the Anti- Vibration facilities into the workstation while at the same time giving overall aseptic work conditions. The Anti-Vibration Table reduces vibrations which often create problems during ICSI procedures. With an isolated microscope platform and a complex anti-vibration system, the K-SYSTEMS Anti-Vibration Table eliminates vibrations from reaching the microscope platform and dampening unavoidable vibrations in the shortest possible time. L126 MP Overall dimensions (WxDxH) 1846x735x2015 mm/73x29x79 Workspace/tabletop (WxD) 1826x553 mm/59x18 Air flow 0,32 m/s Table height 773 mm Order Code The half cylindrical compartment behind the inverted microcopes makes it possible to tilt the illuminator pillar of the inverted microscope - for the exchange of ICSI needles. The space can also accomodate the laser systems or similar products.

10 ivf workstations L124 ICSI Designed as one workplace and prepared for an inverted microscope - for performing ICSI under a laminar flow. The Anti-Vibration table is integrated, working independently of the workstation. L124 ICSI Overall dimensions (WxDxH) 1245x735x2015 mm/49x29x79 Workspace/tabletop (WxD) 1226x553 mm/40x18 Air flow 0,32 m/s Table height 773 mm Order Code

11 ivf workstations The 400 Series Workstations The L400 series is the entry range, accomdating the needs of basic IVF procedures in a lean cost structure. Even though there are fewer optional features, it still guarantees performance and the renowned K-SYSTEMS quality. All workstations have a noise level on only 51 db and an integrated warmed area in the table top based on a stable controlled temperature system. Various heating patterns are available. The table table top is preparred for one or two stereomicroscopes and light source bases. Most stereomicroscopes can be incorporated. Additionally, all models have a stand alone gassing and humidification system GH01- making it possible to avoid large fluctuations in CO2 conditions. Available in 3, 4 and 6 foots lenghts (90, 120 and 180 cm). Two year warranty, includes parts and labor. K-SYSTEMS AluHeat Systems AluHeat was developed by K-systems and is a result of years of experience in manufacturing heat exchangers. AluHeat is an aluminium composite sandwich construction with an integrated warming element and temperature sensors. The warmed mass of aluminium gives off a solid distribution of warmth and secures an even and stable temperature of the table top, which is resistant to thermal shock. Thermal shock is caused by overshooting the temperature when, for example, a cold element is placed on the table top. This typically happens when using electrical heating systems. Advantages such as low maintenance, improved heating of the critical area around the light opening, and the absence of a noisy source of contamination i.,e. water bath, have proven to be invalauble in the more than 200 AluHeat system installed world wide. 11

12 ivf workstations Workstations prepared for one Stereo Microscope L423 IVF Designed as a single workplace and for mounting of a stereomicroscope. 3 foot/90 cm. L424 IVF Designed as a single workplace and for mounting of a stereomicroscope. The microscope can be on either the right or left side. 4 foot/120 cm. L426 IVF Designed as a single workplace and for mounting of a stereomicroscope. The microscope can be either the right or left side. 6 foot/180 cm. L423 IVF L424 IVF L426 IVF Overall dimensions (WxDxH) 940x830x1720 mm 1246x830x1720 mm/49x33x x830x1720 mm/73x33x68 Workspace/Table Top 940x500 mm 1225x500 mm 1825x500 mm Air flow 0,40 m/s 0,32 m/s 0,40 m/s Table height 773 mm 790 mm 773 mm Order Code

13 ivf workstations Workstations prepared for two Stereomicroscopes L426 Dual IVF Designed as two workplaces and for mounting of two stereomicroscopes. A dual workstation offers the potential to simultaneously set up a perfect rotation system / organize division of work and keep the workflow of individuals undisturbed. E.g.: in the morning: one technician collects oocytes while a colleague is already denuding the oocytes from the previous patient. Another situation could be an embryologist inseminating oocytes for conventional IVF, while a colleague is preparing oocytes and sperm to perform ICSI. L426 DUAL IVF Overall dimensions (WxDxH) 1846x830x1716 mm/73x33x68 Workspace/Table Top 1825x490 mm Air flow 0,40 m/s Table height 773 mm Order Code

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15 ivf workstations The 200 Series Workstations The 200 Series are biological safety cabinets - protecting both the product and the operator. For maximum protection of the operator against hazardous or potentially hazardous microorganisms, this microbiological safety cabinet is equipped with a complete air velocity control system. Two HEPA filters as well as recirculation of the air flow (70% recirculation - 30% Exhaust through HEPA-filter) guarantees the safety of operator while working at a BioSafety Cabinet. The average air velocity inside the work chamber is 0,35 m/s. The air velocity is measured with an external aneometer inside the work chamber in an horizontal plane according to the EN12469 Standards. The control panel is conveniently incorporated into the table plate and easy to reach. The table plate is developed to assure a correct work position and a low noise level (< 51dB!) ensure comfort for the operator. There are 2 different models to choose from: L foot (120cm) L foot (180cm) Options for both models include built-in monitor, electrical socket and incorporated UV-light with timer. The K-Systems BioSafety Cabinets are developed according to the standards of the EN12469 Directive - one of the most important directives in the EU for setting the standards for BioSafety Cabinets. As standard, all models have a constant temperature controlled table top prepared for one or two stereomicroscopes and light bases. Additionally, all models have a stand alone gassing and humidification system GH01- making it possible to avoid large fluctuations in CO2 conditions. The class 200 workstation from K-SYSTEMS has easy access for cleaning due to the convenient and accessible tray below the table plate. The advantage is that both microscope light base and microscope can remain mounted on the table top during cleaning. Two year warranty, includes parts and labor. 15

16 ivf workstations Workstations prepared for one Stereomicroscope L224 IVF Designed as a single workplace and for mounting of a stereomicroscope. The microscope can be either right or left sided. 4 foot/120 cm. L226 IVF Designed as a single workplace and for mounting of a stereomicroscope. The microscope can be either right or left sided. 6 foot/180 cm. L224 IVF Overall dimensions (WxDxH) 1246x800x2044 mm/49x32x81 Workspace/tabletop (WxD) 1226x553 mm/40x18 Air flow 0,40 m/s Table height 773 mm Order Code L226 IVF Overall dimensions (WxDxH) 1846x800x2044 mm/73x32x81 Workspace/tabletop (WxD) 1826x553 mm/59x18 Air flow 040 m/s Table height 773 mm Order Code

17 ivf workstations Workstations prepared for two Stereomicroscopes L226 Dual IVF Designed as two workplaces and for mounting of two stereomicroscopes. A dual workstation offers the potential to simultaneously set up a perfect rotation system / organize division of work and keep the workflow of individuals undisturbed. E.g.: in the morning: one technician checks the oocytes for fertilization or scores embryos and transfers them to new dishes. Meanwhile, another technician can prepare the dishes for cryopreservation and runs the cells / embryos to be cryopreserved through the series of cryoprotectants. L226 Dual IVF Overall dimensions (WxDxH) 1846x800x2044 mm/73x32x81 Workspace/tabletop (WxD) 1826x553 mm/59x18 Air flow 040 m/s Table height 773 mm Order Code K-SYSTEMS AluHeat Systems AluHeat was developed by K-systems and is a result of years of experience in manufacturing heat exchangers. AluHeat is an aluminium composite sandwich construction with an integrated warming element and temperature sensors. The warmed mass of aluminium gives off a solid distribution of warmth and secures an even and stable temperature of the table top, which is resistant to thermal shock. Thermal shock is caused by overshooting the temperature when, for example, a cold element is placed on the table top. This typically happens when using electrical heating systems. Advantages such as low maintenance, improved heating of the critical area around the light opening, and the absence of a noisy source of contamination i.,e. water bath, have proven to be invalauble in the more than 200 AluHeat system installed world wide. 17

18 standard features Standard features for K-Systems Workstations Our workstations are designed to meet expectations and to respond to specific demands. Therefore the workstations are provided with a lot of features, some of which are standard and others are as options. The following features are included in the K-Systems Workstations: Vertical Laminar flow Choice of microscopes to be built in (Most microscopes can be built in. Check with your K-Systems distributor) Positions of the microscope, on the right or left. (One center position available for the L423 IVF) Choice of the microscope light base (Note that only K-Systems light base, LS11, LS 12 and LS 14 can be controlled from the control panel on the table plate. Some light sources are subject for additional cost for installation). Vertical laminar air flow To create the aseptic workspace, vertical laminar air flow is used in all cabinets. In the L100/400 series, the air flow is filtered through 2 filter systems, a pre-filter and a HEPA filter. In the L200 series the air is recirculated in a HEPA-filter system where 70% is being recirculated. 99,9995% of all particles down to 0,3µ are removed. The air flow In the class 100 cabinets (series 100, 400) the air flow is taken in from the back of the cabinet, and the air flow is normally 0,31-0,34 m/s. In the Class II cabinets (series 200) the mean airflow velocity to achieve operator protection is at minimum 0.4 m/s, while the mean downflow velocity is m/s. The warmed area of the table top The integrated heating system of the table top is uses Alu Composite Heat Exchanger, where the temperature is Model T-845 R controlled from the control panel at the table top. The variation of the warmed area is ±0,2 C. The temperature setting can be in Celsius or Fahrenheit. The Table Top The table top is made of stainless steel with the warmed area marked as a slightly darker area. To give the best possible working conditions, the front of the table top has been shaped with rounded edge. This ergonomically shaped edge is made to avoid pressure points on the arms of the operator during microscopy. 18

19 standard features The control Panel Integrated in the table plate and easy access for the technician, the control panel permits the control of the following: Fan switch Alarms Heat and Temperature settings Light Source (only K-systems Light sources) Light Timer function Various settings The Workspace The noise off the Fan can be extremely tiring during the day and switching of the fan is tempting, but can threaten the environment around the embryos. One of the advantages of the K-systems Workstations is the low noise level of only 51dBA, achieved through years of experience with Laminar Flow Technique. Inside the cabinet the side panels of hardened glass give a bright and user friendly work environment (only valid for the 100 & 200 Series.). The gassing and humidification system In the series 100, the system is an integrated part of the back wall, using a digital flow meter. For flushing, the humidified and heated gas is diffused through 2 nozzles in the table plate. To maintain the ph in the media, glass incubator hoods can be used (optional). In the L200/400 series the gas humidifier GH01 is used. 19

20 optional features Optional features Incorporated Monitor An excellent tool for viewing, training and education. The integrated 19 glass panel and high resolution monitor permit connectivity to a microscope camera and to an external computer. In dual systems, a switch can be incorporated to switch between two Stereo Microscopes with cameras. The viewing panel of glass is designed to secure a non disturbance of the air flow and is easening the cleaning process. Option only available for all 100 & 200 series. Order Code: Heated Pass Through Tunnel To improve the work flow and to reduce the time of handling oocytes in an unheated and unstable environment, the Heated Pass Through Tunnel is a great alternative to a hatch in the door between the aspiration room and the embryo lab. The Heated Pass Through Tunnel is made of stainless steel with integrated heating elements and a top hinged polycarbonate lid. Warmed Glass Stage Embryos are sensitive to temperature fluctuations. A controlled temperature system can be integrated in the microscope light opening. The temperature system ensures stabile warming of the glass providing uniform warming of the entire warmth area in the Workstation. The system is separately controlled and provides the highest stability of the media in the culture dishes during microscopy. Only available for the 100 & the 400 series. Warming Blocks must not be used on the warmed glass stage. This will damage the warmed glass stage. Order Code: In the cabinet K-Systems provides only the Heated Pass Through tunnel within the workstation. The hatch in the wall of the building remains the responsibility of the customer. Order Code: In the aspiration room The table Top Extra large warmed area As a standard, the table top is partially warmed around the work area of the microscope. As an option, an extra-large warmed area of the workstation can be incorporated. (not possible on L126MP or Dual versions) Available for all IVF workstation series Order code: Depends on the size of you workstation 20

21 optional features Built in Micro Incubator To optimize your work conditions inside the work area, and to reduce the frequency of opening of large CO 2 incubators, a 15 ltr Micro Incubator can be an integrated part of your Workstation. The Micro Incubator has two shelves and a two level alarm system. As an option a glass inner door can be supplied. Available for all 100 series Order code: Electrical Adjustable Height In search of ergonomics and comfort for the technician, K-SYSTEMS Workstaions can be supplied with an electrically adjustable height of the table top. A simple touch button will adjust the height from 694 mm to 1094 mm. Available for all the 100 Series. Order Code: (4-foot model) Order Code: (6-foot model) Electrical outlet inside the cabinet On request, electrical outlets can be provided. Number and placement depends on the type of your Workstation. Order code: UV-Light On request a UV-light with timer can be incorporated. Order code: Particle Counter New workstations can be equipped with a particle counter to monitor the environment in daily operations. Sensors disposed at multiple emplacements will be controlled by software, giving the exact particle count at all times. The size of particles counted are 0,3µ and 0,5µ This feature will also ensure that the HEPA filters are changed when the level of particles becomes critical. 21

22 optional features L100 Series L200 Series L400 Series WORKSTATIONS CONFIGURATIONS L124 IVF L124 ICSI L125 IVF L126 IVF L126 Dual L126 MP L224 IVF L226 IVF L226 Dual L423 IVF L424 IVF L426 IVF L426 Dual Vertical Laminar Flow Preperation for a Stereomicroscope Right Side Preperation for a Stereomicroscope Left Side Preperation for a Stereomicroscope in a Middle Position Preperation for 2 Stereomicroscopes Integrated Humidification System External Humidification System GH01 Integrated Monitor + Humidification System Integrated Incubator + Humidification System Integrated Tunnel + Humidification System Integrated Monitor + Tunnel + Humidification System Integrated Incubator + Tunnel + Humidification System Integrated Incubator + Monitor + humidifcation System Extra Large Heated Area Incoporated UV Light Adjustable Height Incorporation of Electrical Sockets Incorporation of a Light Source LS12 / LS11 Incorporation of a Light Source LS13 External Gassing Incorporation of Heated Glass Light Opening Camera Socket Anit Vibration Table New Developped Features (to be launched soon) Particule Counter Inner Wall Media Precision Thermometer K-Systems Heated Glass Stage 22

23 laminar flow hoods The K-SYSTEMS Laminar Flow hoods - designed for your comfort. The K-systems LAF cabinets set a new standard with respect to comfort and design. The characteristic of the cabinets are the low level of noise from the fan and the bright work environment. Two different series of cabinets are offered: Series 400, a compact model in three different sizes, 90, 120 and 180 cm. Series 200, a Biohazard cabinet, Class II, in two sizes 120 and 180 cm. Two year warranty, includes parts and labor. 23

24 laminar floow hoods Laminar Flow Hoods The class 100 line (400 series) is designed for product protection using vertical air flow. There are 3 different models to choose from: L foot (90cm) L foot (120cm) L foot (180cm) Options for all three models include electrical socket and incorporated UV-light with timer. The L400 Series has a slender, compact and elegant design. With an overall height of only 171 cm, it will easily fit into most laboratories. HEPA filters are a guarantee for particle free air, while easy to reach controls, an ergonomically shaped table plate, and a low noise level (< 51dB!) ensure comfort for the operator. Not only do all of K-Systems Laminar Flow Hoods adhere to the norms and standards which regulate the industry, but we continuously strive to improve operator comfort with respect to ergonomics and noise levels. An elegant design means that not only are these units pleasant to the eye, but their smooth lines enable quick and easy cleaning. Laminar Flow Hoods L 423 Overall dimensions (WxDxH) 940x830x1720 mm/ Order Code L 424 Overall dimensions (WxDxH) 1240x830x1720 mm/49x29x68 Order Code L 426 Overall dimensions (WxDxH) 1846x830x1720 mm/73x29x68 Order Code

25 laminar floow hoods BioSafety Cabinets The 200 Series are biological safety cabinets, for both product and operator protection. Available in 4 and 6 foot lengths (120 and 180cm ). For maximum protection of the operator against hazardous or potentially hazardous microorganisms, this microbiological safety cabinet is equipped with a complete air velocity control system. Two HEPA filters as well as recirculation of the air flow (70% recirculation - 30% Exhaust through HEPA-filter) guarantees the safety of operator while working at a BioSafety Cabinet. The average air velocity inside the work chamber is 0,35 m/s. The air velocity is measured with an external aneometer inside the work chamber in a horizontal plane according to the EN12469 Standards. The control panel is conveniently incorporated into the table plate and easy to reach. The table plate is developed to assure a correct work position and a low noise level (< 51dB!) ensure comfort for the operator. Options for both models include built-in monitor, electrical socket and incorporated UV-light with timer. The K-Systems BioSafety Cabinets are developed according to the standards of the EN12469 Directive - one of the most important directives in the EU for setting the standards for BioSafety Cabinets. Laminar Flow Hoods An elegant design means that not only are these units pleasant to the eye, but their smooth lines enable quick and easy cleaning, due to the convenient and accessible tray below the table top. L 224 Overall dimensions (WxDxH) 1246x735x2015 mm/49x29x79 Order Code L 226 Overall dimensions (WxDxH) 1846x800x2044 mm/73x32x81 Order Code

26 Accessories Warming Blocks To maintain and preserve the temperature in the media during operation, a wide range of warming blocks for dishes, tubes, and syringes are available. The warming block can be used either in your workstation, in the incubators, on warming plates, in dry bath incubators, under incubator hoods or other suitable places. For tubes order code: B01 Round block, holds 7 tubes of 17 mm Falcon B01 B03 B03 Round block, holds 12 tubes of 12 mm Falcon B04 B06 B04 Round block, holds 4 tubes of 25 ml - 23,5 mm B06 Rectangular block for portable incubator, holds 12 tubes of 12 mm, Falcon 2003/NUNC B08 Rectangular block for portable incubator, holds 10 tubes of 17 mm, Falcon B08 B09 B09 Block for swim-up test, 45 angle, holds 2 centrifuge tubes of 17 mm, Falcon For culture dishes B16F Holds two culture dishes - Falcon B16N Holds two culture dishes - NUNC B23 Holds one ICSI dish B24 Holds one center well dish - Falcon B25 Holds one 60 mm culture dish Falcon and NUNC B28 Holds one 4-well dish - NUNC B29 Holds one 5-well dish - MiniTübe 19021/ For syringes B30 Holds 5 flushing syringes of 20 ml / 22,5 mm B35 Holds 5 flushing syringes of 10 ml / 18 mm For media flasks B40 Holds one IVF MediaFlask of 125 ml B16FB16N B24 B28 B30 B23 B25 B29 B40 26

27 Accessories Incubator Hoods A tool to protect the media from being cooled by any air flow. By using the glass incubator hood the media in the culture dishes are continuously flushed in order to maintain the ph and at the same time avoid osmolarity. In the 100 series you will only need to place the incubator hoods over the nozzles in the table top. In the series 400IVF and the 200 IVF the incubator hood is connected to the table top humidifier and gassing unit by a silicon tube. Glass incubator hoods order code: 100 series G101 For gassing culture dishes Diameter 140 mm height 75 mm G102 For gassing media and sperm samples Diameter 130 mm height 130 mm G103 For gassing culture dishes Diameter 140 mm height 25 mm Glass incubator hoods order code: 200IVF & 400E series G01 For gassing culture dishes Diameter 140 mm height 75 mm G02 For gassing media and sperm samples Diameter 130 mm height 130 mm G03 For gassing culture dishes Diameter 140 mm height 25 mm G101 G01 G102 G103 G02 G03 27

28 light sources Light Sources for Stereo Microscopes The K-SYSTEMS light sources provide correct illumination, which is a key to informative investigation and inspection during microscopy. Choosing the correct light source provides better illumination while more details become visible, leading to more reliable inspection and documentation. The K-SYSTEMS Light sources are designed to fit all stereomicroscopes built into the K-SYSTEMS Workstations. They are conveniently mounted underneath the table top leaving a smooth and even surface above. The light intensity is adjustable from the control panel on the table plate of the Workstation. LS11 Overall dimensions (WxDxH) 495x325x810 mm Material Aluminium Main voltage VAC Power consumption 20 W Bulb 12V Max 20W; SP 10 C Lux 5000 cd Order Code LS12/LS14 Overall dimensions (WxDxH) 277x120x96 mm Material Aluminium Main voltage VAC Power consumption 20W Bulb 12V Max 20W; 10 C Lux 5000 cd Order Code Red filter Green filter Blue filter LS 11 the basic Light source LS11 is a well tested and rugged light source equipped with a 360 degree tiltable, hollow and plane double mirror, enhancing inspection of different morphological characteristics. The hollow mirror provides a uniform distribution of light when working at low magnifications. The light from the halogen light bulb is filtered through frosted glass before the two mirrors. The LS11 is well suited for routine procedures. LS12 The advanced Light Source The K-systems high end illumination system features two condenser lenses, a 360 degree rotational and 40 mm horizontally moveable two sided mirror and a range of optional colour filters. The horizontally moveable mirror is the optimal tool to fine adjust shades for viewing transparent objects, i.e. follicular fluid for oocytes. The hollow mirror provides a uniform distribution of light when working at low magnification and the plane double mirror allows performing standard inspections at high magnification. LS 14 The Advanced Light source for Class 2 Workstations. The LS14 is identical with a LS12 but developed for the Class 2 IVF Workstations, having a larger light opening giving full unlimited and undisturbed illumination. This high end illumination system features two condenser lenses, a rotational and horizontally movable two sided mirror and optional colour filters. The K-Systems LS14 light source is an excellent choice where ideal light quality, high light output, completely uniform illumination and coloured filters are required. 28

29 laminar flow hoods Free Standing Warming Table prepared for the incorporation of a Stereo Microscope The free standing table features a warmed area similar to what is offered in our Workstations. Additionlly, the warming table is preparred for a stereo microscope, control panel is integrated in the table top, and light source is incorporated below the table top. The heating system is the AluHeat, built in as standard. Most Stereo Microscopes can be incorporated and the light intensity from any K-systems light source can be adjusted from the control panel. The free standing table is intended for use in any laboratory requiring a warmed area in the table top, and often to be used in laboratories built as HEPA filtered clean room with over pressurized facilities. K-SYSTEMS AluHeat Systems AluHeat was developed by K-systems and is a result of years of experience in manufacturing heat exchangers. AluHeat is an aluminium composite sandwich construction with an integrated warming element and temperature sensors. The warmed mass of aluminium gives off a solid distribution of warmth and secures an even and stable temperature of the table top, which is resistant to thermal shock. Thermal shock is caused by overshooting the temperature when, for example, a cold element is placed on the table top. This typically happens when using electrical heating systems. L524 Overall dimensions (WxDxH) 1241x725x863 mm/48,9x28,6x34 Order Code Advantages such as low maintenance, improved heating of the critical area around the light opening, and the absence of a noisy source of contamination i.,e. water bath, have proven to be invalauble in the more than 200 AluHeat system installed world wide. 29

30 anti vibration tables Anti Vibration Tables - designed to meet the requirements of ICSI procedures Vibrations are elimininated by a complex anti vibration system achieved through an optimized choice of mass and support stiffness ensuring a fast dampening effect. The inverted microscope is placed on an equilibrated and isolated platform, which gives a smooth and roomy workspace around the microscope for micromanipulation equipment, mini-incubators, patient records etc. The table is adjustable for all available models of inverted microscopes including motorized models. Two versions of the Anti Vibration table are available, 120 cm and 82 cm. The microscope platform of the K-SYSTEMS Anti- Vibration Table does not touch the actual table. The result is that vibrations reaching the table do not affect the platform. av1 AV2 Overall dimensions (WxD) 1200x800 mm 820x700 mm Material Stainless steel Stainless steel Height of table 780 mm 780 mm Support stiffness, approx Nm Nm Mass (to be dampened) Adjustable Adjustable Order Code Optional shelf (Order Code) Optional monitor on arm (Order Code) Displacement Reverberation of the anti-vibration system Time 30

31 gas mixer On site mixing of CO2 in air or Tri-gas with advanced technology The outstanding design of the gas mixer from K-systems offers a compact unit giving you a steady supply of properly mixed high-quality gas. The mixer is suitable for a range of applications and procedures within IVF or other fields in need of controlled gas mixing. It can be used as either a mixer for CO2 in air or for mixing three gasses, carbon dioxide, nitrogen and oxygen. The mixer works by balancing pressures from the input gas supplies to ensure that the component gasses are mixed at the same pressure. The unit provides constant mix control for a range of flow and pressure conditions. The mixer enables precise flow control at the low flow rates needed within IVF and has a capacity up to 29 l/h. The unit is self calibrating and has 3 external HEPA filters for easy exchange, as well as an audible alarm and RS 232 port for data logging. The mixer is supplied with a factory pre-setting according to pre-given specifications. GM3 Overall dimensions (WxDxH) 480x400x100 mm/19x16x4 Temperature range ambient - 49 C Temperature accuracy ± 0,2 C Gas pressure 0,5-1,0 bar Gas consumption, flushing 5-10 litres per/h Flow range 0-29 litres per/h Power consumption 80W Heating 70W Alarms (audible and visble) Out of range temperature and air flow Order Code

32 warming plates Slim Line Warming Plate in Elegant Design with Integrated Controller The K-SYSTEMSTM T47E Warming Plate has a compact and elegant design which fits into any lab. The T47E is a multi-purpose warming plate with a hardened double-anodized surface, making it chemically resistant and easy to clean. As an option, an external sensor can be included to assure a correct temperature in the media. The sensor is placed in a test tube mounted in a warming block on the warming plate, and the temperature level is adjusted accordingly on the control panel. The built-in RS232 communication port provides the option of monitoring and recording temperature stability with a computer. The K-SYSTEMS wide range of warming blocks makes it a flexible partner for a variety of laboratory procedures. The easy-to-read temperature is extremely precise and reliable, within the warming plate s range of ambient to 45 C. The temperature level is adjusted directly on the LED display in increments of 0,1 C. The integrated controller in the back of the warming plate. T47E Overall dimensions (WxDxH) 470x460x6 mm Material Aluminium Heated area 470x400 mm Heating element 24V, 85W Temperature range Ambient - 45 C Temperature accuracy ± 0,3 C Power supply, 24V adapter for mains: 24V DC Order Code

33 incubators Trolley with warmed table top and heated drawer designed for the aspiration room The K-SYSTEMS R65ET is a trolley with a warmed table top that provides optimal temperature control of utensils, media and follicular fluid during oocyte retrieval. The elegantly designed mobile trolley includes a warmed surface and a heated drawer below, which enables instruments to be kept warm. The warmed surface is temperature adjustable within a range from ambient - 45 ºC. The temperature can be monitored on the digital display, and is adjusted directly on the control panel in increments of 0,1 C. Four swivel castors, two of them with brakes, allow for convenient positioning of the trolley and more efficient use of limited space. r Overall dimensions (WxDxH) 664x581x777 mm Material Stainless steel Warming area 450x380 mm Table top 661x493 mm Height 777 mm, incl wheels Weight 25 kilos Power supply 230/115V + 6/50 Hz Power consumption 200W Temperature range ambient - 45 C Temperature accuracy ± 0,3 C Order Code

34 gassing humidifier Trolley with Warmed Table Top - designed for the apiration room The stainless steel trolley has a partially warmed table top. The temperature is adjustable within a range of ambient - 45 ºC. The control panel has a digital reading for actual temperature and set temperature. The trolley is provided with 4 swivel castors, two with brakes. Combined with warming blocks, the warmed table top is useful for maintaining a correct temperature of utensils, media and follicular fluid during oocyte retrieval. Gassing Humidifier The GH01 is a small and convenient stand alone humidifier. It has a built in digital flow meter and an adjustable of flow rate between 0 50 litres per hour. The flow rate is adjusted on the analog proportional needle valve, positioned on the side of the cabinet for easy access. The water for humidification of the gas is heated up to 39,5 C by a 20W heating element. This level of temperature ensures approximately 37,5 C at the exit point of the supplied length of tubing. The bottle can be removed for cleaning or autoclaving, and comes with a choice of either a diffuser or an open end. The diffuser creates the best possible humidification. Due to the physical laws, this can result in some condensation in the tubing. By using an open end the humidification level is lowered and less condensation occurs. The design of the GH01 ensures an easy airflow over the unit, so that it can be used in a laminar flow workstation without causing turbulence. The low gravity point makes sure that the unit is stable and doesn t tilt during use. ret The humidifier can be used with CO 2, Oxygen, Nitrogen or any mixture of these gasses. The GH01 comes with a separate power supply and operates on low voltage for safe conditions, for instance if water is spilled. Overall dimensions (WxDxH) 640x450x760 mm Material Stainless steel Height 760 mm, incl trolley Heated area 450x380 mm Heating element 24V, 85W Temperature range Ambient - 45 C Temperature accuracy ± 0,3 C Power supply 24V DC 5A Power consumption mains 90W Order Code GH01 Overall dimensions (WxDxH) 175x50x126 mm Material Stainless steel Gas consumption 0-50 litres per hour (adjustable) Gas pressure 0,5-0,7 bar Power supply, adapter for mains: 15V Power consumption mains 6VA Order Code

35 incubators CO2 Mini Incubator for optimal ph and Temperature control. Maintaining cell viability of gametes and embryos while outside of main CO2 incubators is critical. The K-SYSTEMS bench top incubator, G85, optimizes culture conditions and avoid severe fluctuations in temperature, ph and light conditions as for instance during ICSI. The small size of the unit allows it to be placed near the actual workplace for instance on the anti vibration table. Due to the small size of each of the two chambers and a heated lid the correct temperature and gas levels are quickly re-established after opening, by using the purge function. The G85 has an integrated digital gas flow meter, electrical heating in the chamber lids, gas flushing, a purge function and a humidifier. B16N Holds two Nunc center-well dish B16F Holds two Falcon center-well dish B23 Holds one ICSI dish B24 B25 Holds one center-well dish Falcon Holds one 60 mm culture dish Falcon and NUNC B28 Holds one 4-well dish - NUNC B29 Holds one 5-well dish MiniTüb 19021/ The control panel is easy to use and the display is easy to read. The design of the unit makes it practical with respect to cleaning. By using the G85, stress to cells due to irregular temperature and ph is avoided, while at the same time minimizing the number of times one needs to open the main CO2s incubators, thereby helping to maintain temperature and ph levels in these units. B16F/B16N B23 B24 B25 B28 B29 G85 Overall dimensions (WxDxH): 267x337x90 mm Weight incl. battery: 3.5 kilos Material: Stainless steel Power supply: 24V DC Power consumption: 120 W Heating element: 24 V, 115 W Temperature range: Ambient to 45 C Temperature accuracy: ±0.2 C Flow range: 0-50 Litres/h Gas consumption: 5-10 litres per hour Gas pressure: bar Alarms: Audible and visible for out of range temperature and air flow Order Code:

36 incubators Portable Incubator for Safe Transport of gametes and embryos With K-SYSTEMS portable incubator G95, gametes and embryos are protected from fluctuations in temperature, ph and light conditions during transport. The G95 protects samples 100% from light, while maintaining a correct and stabile temperature due to the built in battery, which ensures a correct temperature level for a minimum of 4 hours. The G95 can be connected to the cigarette lighter socket in an automobile for longer transport. The control panel for setting and reading of the temperature is mounted on the front. The temperature range can be set from ambient - 45 C with an accuracy of ± 0,2 C. The gas level will drop approx. 1% CO2 per hour (based on a start level of 5% CO2 at 37 C). Samples in transport are normally sealed, and since the surface area compared to the volume of media is little, the gas exchange between the sample and the atmosphere inside the incubator is minimal. Resulting in an acceptable drop in gas level. The G95 Portable Incubator has an elgant design, while at the same time practical with respect to cleaning. The control panel is easy to use and the display is easy to read. This unit is an excellent choice for satellite labs or in the laboratory as a substitute for the K-Systems G73 Dry Bath Incubator. Note that most K-Systems warming block fit both the G95 Portable and the G73 Dry Bath Incubator. Usage: Culture dishes and/or test tubes are placed in interchangeable warming blocks which are held in fixed positions during transport. The chamber is purged with the desired gas mixture (regulated for instance by the GH01) - During purging, the door is closed but only sealed after purging. 36

37 incubators The gas supply is disconnected from the quick-snap valve located on the back of the G95. Should the transport time exceed the charge time of the built in battery, the G95 can be connected to cigarette lighter socket found in most automobiles. Portable Incubator - G96 K-SYSTEMS Portable incubator G96, allows transport of cultures, gametes and embryos over longer distances. The G96 incubator is equipped with durable Lithium Ion battery pack which allows usage for more than 24 hours. The incubator is reliable and will operate on 12 volts, battery or mains either in your car, plane or laboratory. The G96 incubator will give you a full report of the events after usage. Connect the incubator to your palm pilot running the K-Systems software module, and you have full control of events happened during transport. The memory in the incubator will store your data for later retrieval. Through a gas port, the incubator can be connected to a gas feed of your choice and will sustain the gas level during transport for several hours. G95/G96 Chamber dimensions (WxDxH) 160x105x130 mm Volume 2,2 litres Overall dimensions 245x220x315 mm, incl handle Weight incl.battery 4,7 kilos Material Stainless steel/aluminium Temperature range ambient - 45 C Temperature accuracy ± 0,2 C Temperature set/read Digital display LED Gassing, ready made mixture 5% CO 2 in-air or the like Gas pressure 0,5-1,0 bar Gas consumption, flushing 30 litres per/h Gas flushing time 15 minutes Power supply, 24V adapter for mains: 115/230V, Hz Power consumption mains 70W Power supply, car 12V cigarette lighter socket Power consumption car: 30W Fuse 4,0 amps slow blow Heating element 30V, 20W Built-in-battery 14,8V Total charging time 3 hours (8 hours G96) Alarms Battery, temperature CO 2 holding time 1% drop. per hour Order Code

38 incubators Dry Bath Incubator for heating samples in test tubes The K-SYSTEMS Dry Bath Incubator, G73, is ideal for all sample tubes requiring a stable heated environment. The highly visible digital LED display indicates the temperature level in either Celsius or Fahrenheit. The temperature is extremely precise and reliable within the incubator s range of ambient to 45 C. This is due to the PID controller, which ensures a temperature accuracy of +/- 0,2 C. The temperature level is adjusted directly on the display in increments of 0,1 C. This elegantly designed, aerodynamic block warmer has a surface of easy-to-clean anodised aluminium. The built in RS232 communication port provides the option of monitoring and recording temperature stability with a computer. Our wide range of heating blocks makes the G73 Dry Bath Incubator a flexible partner for a variety of different laboratory procedures. G73 Overall dimensions (WxDxH) 180x140x85 mm Material Stainless steel Heated area 120x80 mm Heating element 24V, 20W Temperature range Ambient - 45 C Temperature accuracy ± 0,2 C Power supply, 24V adapter for mains: 24V DC, 2 Amp. Power consumption mains 25 Watt Order Code

39 freeze control systems CO 2 Incubators Galaxy R+ series The Galaxy R+ is a 170 liter CO2 incubator featuring a graphical display on the front door providing the user with important data like temperature, CO2 over the past 72 hours etc. The model is fitted with unique dual chamber heating system to optimise temperature recovery throughout the chamber. The unit features a range of options including three or six inner doors, oxygen control and improved high temperature decontamination cycle with validated results. The Galaxy R+ incorporates a viewing door system and a large range of options and accessories. Order code Galaxy S+ series The Galaxy S+ is a 170 liter CO2 incubator featuring many of the options from the R+ series. Galaxy S+ has a sophisticated design with rugged reliability and key areas of technical superiority. The unit can be fitted with the improved high temperature decontamination cycle. Order code Galaxy B+ Series The Galaxy B+ Series has a single door entry system to simplify access to the 170 liter chamber. The model is fitted with seamless inner chamber with fully rounded corners and has an easily removable shelving system. This minimizes the number of surfaces and components to be cleaned. Order Code Galaxy Mini A The Galaxy Mini A is a 50 liter CO2 incubator and has one heated doubled glazed door with an integral viewing window (optional split inner door available). The unit has a homogeneous environment of temperature, CO2 and humidity to provide optimum cell growth conditions without the need for fan circulation. Featuring same options as Galaxy R. Order code Galaxy Micro The 14 Litre Galaxy Micro CO2 Incubator for procedures that demand accurate temperature and CO2 levels with quick and convenient incubator access. Incorporate the Micro Galaxy directly in a K-SYSTEMS L100 Workstation for easy access when performing in Vitro Fertilisation (IVF) techniques. It is compact and stackable, optimizing laboratory space. The small sized chamber is equipped with and infrared sensor for faster and accurate CO2 recovery. Order Code:

40 freeze control systems Cryopreservation Systems - Freezing of biological material for long term preservation and viable recovery CL-5500 Order code: CL-8800 Order code: Calibrations for temperature measurements and for control are performed to within ±0,1 C. Cryochambers - The chamber design ensures symmetrical heat transfer from all specimens to Liquid Nitrogen The conductive core of the chamber, and the tight thermal coupling between specimens and core ensure that Latent Heat is efficiently removed during the nucleation process. FREEZE CONTROL systems from CryoLogic Pty. Ltd. are controlled-rate, Liquid Nitrogen freezers precision engineered for cryopreservation of biological specimens. Each Systems consists of a temperature controller, croychamber, and cryobath. The chamber stands directly in Liquid Nitrogen ensuring maximum heat transfer. The connected controller regulates the temperature of the specimens within ±0,1 C. Temperature Controllers FREEZE CONTROL temperature controllers provide reliable and accurate temperature regulation of specimens. Temperature protocols are precisely followed and temperatures can be held constant at any point within the control range. Specimen temperature is monitored continuously, and is measured by a precision platinum temperature sensor permanently mounted in the core. Specimens can be safely accessed for manual seeding, without exposing them to temperature fluctuations. A range of FREEZE CONTROL cryochambers is available to accommodate different applications, with cooling rates appropriate for most protocols. Chambers are interchangeable because each chamber is separate from, and plugs into the controller. Customers select the options most appropriate to their needs. CL-3300 Order code: CL-2200 Order code: Order code:

41 freeze control systems Cryobaths - CryoBaths are insulated Liquid Nitrogen containers, available in two capacities The large CryoBath has a capacity of 3.8 Litres, allowing approximately 4 hours of continuous operation before refilling is required. The small CryoBath has a capacity of 1.5 Liters, giving approximately 2 hours of run time before a fill is needed. Order Code: 1.5 Litres Order Code: 3.8 Litres CryoChamber Lids Available in three sizes, to suit a wide variety of straws. Order Code: (standard lid) CryoLogic Options - CryoGenesisTM V5 Temperature Control Software Dedicated Windows based software for programmable freezers. Allows the user to simply manage freezing jobs including setup and edit job details, design temperature protocols, run a job allowing user interventions, log actual temperature progress and review a history of all job information. Order Code: Carry Case Horizotal or Upright construction. Rugged construction for shipping and storage, weight with system 9-15 kg. Order Code: Power Pack Battery power supply for CL8800, CL2200 and CL3300 controllers. Mains back-up or stand alone support for up to 3 hours. Operates from its internal battery, from mains or from external 12Volts source. Order Code:

42 freeze control systems Cryologic Vitrification Method CryoLogic has developed a simple, convenient, and reliable process, the CryoLogic Vitrification Method (CVM), for vitrification of specimens. CVM is a safe, simple, and convenient procedure which offers a number of advantages over other vitrification methods. CVM involves ultra-rapid cooling of specimens without their immersion in, or direct contact with, liquid nitrogen. This avoids any potential contamination by pathogenic micro-organisms that may be present in liquid nitrogen. CVM also avoids barriers to heat transfer caused by the nitrogen vapor, or by straws. Specimens can be conveniently handled, stored, and ultra-rapidly thawed. Minimal thawing medium is required and specimens can be easily located after thawing. Order Code:

43 future products Future products from K-SYSTEMS As the IVF market constantly seeks new ways to improve procedures and results, K-systems R&D division is constantly working on meeting this demand by developing new products. L323, IVF IncuChamber Exposure of human oocytes and embryos to room air and to room temperature can affect their viability. The IVF Incubator Chamber is developed as a semi open system allowing you to perform oocyte screening, feritlization, identification, cleavage check and embryo transfer in an incubator like environment. The IncuChamber maintains in the procedure area a constant temperature, humidity, CO2 and oxygen level. Warmed, HEPA filtered air is recirculated inside the chamber where table top surface is temperature controlled. CO2, oxygen and temperature can easily be monitored from read outs in the wall. Loading hatches are side and front mounted as well as two access ports which are mounted in front of the Stereo Microscope. Stereo Microscope is integrated in the table top and light source mounted below. User defined temperature settings, timer functions and adjustable light intensity can be operated from the operation panel in front of the table top. 43

44 future products The G185 - Vitro Cube The Vitro-Cube is a desktop humidified Tri-gas and CO2 incubator used for maintaining an optimal environment for the development of embryos. The Vitro-Cube has a capacity of up to 20 NUNC or FALCON 4-well culture dishes in 10 separate compartments, each with its own lid. The Vitro-Cube has an additional large-sized compartment that can hold test tubes and culture media. The logging is shown on the graphical display or can be downloaded to a PC via a serial interface. A dialer will call up to 5 telephone numbers and leave a message if any alarm conditions should occur. The Vitro-Cube ensures a correct and stable environment with respect to temperature and gassing levels. The gas supply to a compartment can be shut off when not in use, without interfering with the gas supply to the active compartments. The top of the Vitro-Cube containing all the compartments, is detachable and autoclaveable. The integrated gas mixer is all microprocessor-controlled. The gas is heated and humidified in a autoclaveable flask supplied with the Vitro-Cube. The Vitro-Cube has full datalogging capabilties with usercontrolled logging of the temperature, gas mixture and alarm conditions. Warmed Glass Stage K-SYSTEMS expect to launch a new warmed glass stage to ensure optimal temperature control during microscopy. Due to the thickness of the glass, a higher consistency in temperature level can be achieved and an improved distribution of heat. The glass fits into all light openings of Ø90 mm and is a very economical solution. G185 Overall dimensions (WxDxH) 850x558x152,5 mm Order Code

45 A Adjustable height 21 AntiVibration tables 30 B BenchTop Incubator 35 Block Warmer 38 C CO2 Measuring Unit 39 Cryologic 40 D DryBath Incubator 38 E Electrical outlet 21 F Freeze Control Systems 40 G Galaxy Incubators 32 Gassing Humidifier 34 S Screen 20 Series Series 400 IVF/standard 12/24 Series 200 IVF/standard 15/25 T TokaiHit 20 Tunnel 20 U UV-Light 21 V Vertical laminar flow 18 W Warming blocks 26 - for dishes - for Tubes - for Syringes Warming plate 32 Warming Table 33 H Heated Trolley 33 Heated Glass Stage 20 I Incubators 35 Incubator hoods 27 IVF Workstations 5 L Laminar flow hoods 23 Large CO2 Incubators 39 Light sources 28 M MicroIncbator 21 Monitor 20 P Pass Through 20 45

46 K-SYSTEMS - Kivex Biotec Ltd Klintehøj Vænge 3-5 DK-3460 Birkerød, Denmark Tel.: Fax: info@k-systems.dk K-SYSTEMS All rights reserved