Waters Xevo G2-S QTof Operator s Overview and Maintenance Guide

Transcription

1 Waters Xevo G2-S QTof Operator s Overview and Maintenance Guide Revision B Copyright Waters Corporation 2013 All rights reserved

2 Copyright notice WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER. The information in this document is subject to change without notice and should not be construed as a commitment by Waters Corporation. Waters Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. Trademarks ACQUITY, ACQUITY UPLC, Connections INSIGHT, ESCi, UPLC, and Waters are registered trademarks of Waters Corporation. IntelliStart, LockSpray, MassLynx, NanoFlow, NanoLockSpray, StepWave, T-Wave, THE SCIENCE OF WHAT'S POSSIBLE., UNIFI, Xevo, and ZSpray are trademarks of Waters Corporation. GELoader is a registered trademark of Eppendorf-Netheler-Hinz GmbH. PEEK is a trademark of Victrex plc. POZIDRIV is a registered trademark of Phillips Screw Company, Inc. Swagelok is a registered trademark of Swagelok Company. Super Flangeless and SealTight are trademarks of Upchurch Scientific, Inc. TaperTip is a trademark of New Objective, Inc. Teflon and Viton are registered trademarks of E. I. du Pont de Nemours and Company. Valco is a trademark of Valco Instruments, Inc. Xylan is a registered trademark of Whitford Corporation. Other trademarks or registered trademarks are the sole property of their respective owners. ii Februrary 11, 2013, Rev. B

3 Customer comments Waters Technical Communications department invites you to tell us of any errors you encounter in this document or to suggest ideas for otherwise improving it. Please help us better understand what you expect from our documentation so that we can continuously improve its accuracy and usability. We seriously consider every customer comment we receive. You can reach us at Contacting Waters Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information: Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide. Visit Telephone From the USA or Canada, phone HPLC, or fax For other locations worldwide, phone and fax numbers appear on the Waters Web site. Conventional mail Waters Corporation 34 Maple Street Milford, MA USA Februrary 11, 2013, Rev. B iii

4 Safety considerations Some reagents and samples used with Waters instruments and devices can pose chemical, biological, and radiological hazards. You must know the potentially hazardous effects of all substances you work with. Always follow Good Laboratory Practice, and consult your organization s safety representative for guidance. Considerations specific to the Xevo G2-S QTof Solvent-leakage hazard The source exhaust system is designed to be robust and leak-tight. Waters recommends you perform a hazard analysis, assuming a maximum leak into the laboratory atmosphere of 10% LC eluate. Warning: To avoid personal contamination with toxic or biologically hazardous materials, you must follow these safety measures: To confirm the integrity of the source exhaust system, renew the source O-rings at intervals not exceeding one year. To avoid chemical degradation of the source O-rings, which can withstand exposure only to certain solvents (see Solvents used to prepare mobile phases on page 253), determine whether any solvents you use that are not listed are chemically compatible with the composition of the O-rings. Flammable-solvents hazard Warning: To prevent the ignition of accumulated solvent vapors inside the source, maintain a continuous flow of nitrogen through the source whenever significant amounts of flammable solvents are used during the instrument s operation. Never let the nitrogen supply pressure fall below 414 kpa (4.0 bar, 60 psi) during analyses that require flammable solvents. Connect to the LC output with a gas-fail connector to stop the LC solvent if the nitrogen supply fails. iv Februrary 11, 2013, Rev. B

5 Glass-breakage hazard Warning: To avoid injuries from broken glass, falling objects, or exposure to toxic substances, never place containers on top of the instrument or on its front covers. Instead, use the bottle tray. High-temperature hazard Warning: To avoid burn injuries, do not touch the source ion block assembly when operating or servicing the instrument. Xevo G2-S QTof high-temperature hazard: Source ion block assembly Februrary 11, 2013, Rev. B v

6 Hazards associated with removing an instrument from service When you remove the instrument from use to repair or dispose of it, you must decontaminate all of its vacuum areas. These are the areas in which you can expect to encounter the highest levels of contamination: Source interior Waste tubing Exhaust system Rotary pump oil (where applicable) The need to decontaminate other vacuum areas of the instrument depends on the kinds of samples the instrument analyzed and their levels of concentration. Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible. Management must also prescribe the method of decontamination to be used and the appropriate protection for personnel undertaking the decontamination process. You must handle items such as syringes, fused silica lines, and borosilicate tips used to carry sample into the source area in accordance with laboratory procedures for contaminated vessels and sharps. To avoid contamination by carcinogenic, toxic, or biohazardous substances, you must wear chemical-resistant gloves when handling or disposing of used oil. FCC radiation emissions notice Warning: To avoid personal contamination with biologically hazardous, corrosive, or toxic materials, wear chemical-resistant gloves during all phases of instrument decontamination. Warning: To avoid puncture injuries, handle syringes, fused silica lines, and borosilicate tips with care. Changes or modifications not expressly approved by the party responsible for compliance, could void the users authority to operate the equipment. This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation. vi Februrary 11, 2013, Rev. B

7 Canada spectrum management emissions notice This class A digital product apparatus complies with Canadian ICES-003. Cet appareil numérique de la classe A est conforme à la norme NMB-003. Electrical power safety notice Do not position the instrument so that it is difficult to operate the disconnecting device. Safety hazard symbol notice Documentation needs to be consulted in all cases where the symbol is used to find out the nature of the potential hazard and any actions which have to be taken. Equipment misuse notice If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Safety advisories Consult Appendix A for a comprehensive list of warning and caution advisories. Operating this instrument When operating this instrument, follow standard quality control procedures and the guidelines presented in this section. Februrary 11, 2013, Rev. B vii

8 Applicable symbols Symbol Definition Manufacturer Authorized representative of the European Community Confirms that a manufactured product complies with all applicable European Community directives Australia C-Tick EMC compliant Confirms that a manufactured product complies with all applicable United States and Canadian safety requirements Consult instructions for use Contact Waters Corporation for the correct disposal and recycling instructions Audience and purpose This guide is for operators of varying levels of experience. It gives an overview of the instrument, and explains how to prepare it, change its modes of operation, and maintain it. Intended use Waters designed the orthogonal acceleration, time-of-flight Xevo G2-S QTof for use as a research tool to deliver authenticated mass measurement. The Xevo G2-S QTof is for research use only and is not intended for use in diagnostic applications. viii Februrary 11, 2013, Rev. B

9 Calibrating To calibrate LC systems, follow acceptable calibration methods using at least five standards to generate a standard curve. The concentration range for standards should cover the entire range of QC samples, typical specimens, and atypical specimens. When calibrating mass spectrometers, consult the calibration section of the operator s guide for the instrument you are calibrating. In cases where an overview and maintenance guide, not operator s guide, accompanies the instrument, consult the instrument s online Help system for calibration instructions. Quality control Routinely run three QC samples that represent subnormal, normal, and above-normal levels of a compound. Ensure that QC sample results fall within an acceptable range, and evaluate precision from day to day and run to run. Data collected when QC samples are out of range might not be valid. Do not report these data until you are certain that the instrument performs satisfactorily. ISM classification ISM Classification: ISM Group 1 Class A This classification has been assigned in accordance with CISPR 11 Industrial Scientific and Medical (ISM) instruments requirements. Group 1 products apply to intentionally generated and/or used conductively coupled radio-frequency energy that is necessary for the internal functioning of the equipment. Class A products are suitable for use in all establishments other than domestic and those connected to a low-voltage power supply network that supplies buildings used for domestic purposes. Februrary 11, 2013, Rev. B ix

10 EC authorized representative Waters Corporation (Micromass UK Ltd.) Floats Road Wythenshawe Manchester M23 9LZ United Kingdom Telephone: Fax: Contact: Quality manager x Februrary 11, 2013, Rev. B

11 Table of Contents Copyright notice... ii Trademarks... ii Customer comments... iii Contacting Waters... iii Safety considerations... iv Considerations specific to the Xevo G2-S QTof... iv FCC radiation emissions notice... vi Canada spectrum management emissions notice... vii Electrical power safety notice... vii Safety hazard symbol notice... vii Equipment misuse notice... vii Safety advisories... vii Operating this instrument... vii Applicable symbols... viii Audience and purpose... viii Intended use... viii Calibrating... ix Quality control... ix ISM classification... ix ISM Classification: ISM Group 1 Class A... ix EC authorized representative... x 1 Waters Xevo G2-S QTof Overview Waters Xevo G2-S QTof IntelliStart technology ACQUITY and nanoacquity Xevo G2-S QTof UPLC/MS systems Software and data system Februrary 11, 2013, Rev. B xi

12 LockSpray source and ionization modes Electrospray ionization (ESI) Atmospheric pressure chemical ionization (APCI) Combined electrospray/atmospheric pressure chemical ionization (ESCi) Atmospheric solids analysis probe (ASAP) NanoLockSpray source and ionization modes TRIZAIC UPLC source Combined APPI/APCI source IntelliStart Fluidics system IntelliStart Fluidics system physical layout System operation Ion optics Leak sensors Vacuum system Preparing the Mass Spectrometer for Operation Starting the mass spectrometer Verifying the instrument s state of readiness Monitoring the mass spectrometer LEDs Calibration Flow rates for the Xevo G2-S QTof system Preparing the IntelliStart Fluidics system Installing the reservoir bottles Adjusting the solvent delivery tube positions Purging the pump xii Februrary 11, 2013, Rev. B

13 Rebooting the mass spectrometer Leaving the mass spectrometer ready for operation Emergency shutdown of the mass spectrometer Configuring the LockSpray Source Configuring the LockSpray source Configuring for ESI mode Installing the ESI probe Removing the ESI probe Configuring for APCI mode Installing the IonSABRE II probe Removing the IonSABRE II probe Configuring for ESCi mode Optimizing the ESI probe for ESCi operation Configuring the NanoLockSpray Source Overview of the NanoLockSpray source Sample sprayer LockSpray sprayer NanoFlow gas supply Purge gas Sprayer platform adjuster assembly Februrary 11, 2013, Rev. B xiii

14 Selecting and configuring the NanoLockSpray source Deploying the sprayer platform adjuster assembly Adjusting the sprayer tip position Setting up the camera Optional glass capillary sprayer Maintenance Procedures Maintenance schedule Spare parts Troubleshooting with Connections INSIGHT Safety and handling Preparing the instrument for working on the source Removing and refitting the source enclosure Removing the source enclosure from the instrument Fitting the source enclosure to the instrument Installing and removing the corona pin Installing the corona pin in the source Removing the corona pin from the source Operating the source isolation valve Removing O-rings and seals Cleaning the instrument case Emptying the nitrogen exhaust trap bottle Maintaining the roughing pump Maintaining the Oerlikon Leybold oil-filled roughing pump Gas ballasting the Oerlikon Leybold roughing pump Inspecting the roughing pump oil level Adding oil to the roughing pump Replacing the roughing pump s oil and oil demister elements xiv Februrary 11, 2013, Rev. B

15 Cleaning the source components Cleaning the sampling cone assembly Removing the sampling cone assembly from the source Disassembling the sampling cone assembly Cleaning the sample cone and cone gas nozzle Assembling the sampling cone assembly Fitting the sampling cone assembly to the source Cleaning the ion block assembly Removing the ion block assembly from the source assembly Disassembling the source ion block assembly Cleaning the ion block components Assembling the source ion block assembly Fitting the ion block assembly to the source assembly Cleaning the StepWave ion guide assembly Handling the StepWave ion guide assembly Removing the ion block support from the source assembly Removing the StepWave assembly from the source assembly Disassembling the StepWave ion guide assembly Cleaning the StepWave ion guide assembly Assembling the StepWave ion guide assembly Fitting the StepWave assembly to the source assembly Fitting the ion block support to the source Replacing the ESI probe tip and gasket Removing the ESI probe tip and gasket Fitting the ESI probe tip and gasket Replacing the ESI probe sample capillary Removing the existing capillary Installing the new capillary Cleaning the IonSABRE II probe tip Replacing the IonSABRE II probe sample capillary Removing the existing capillary Installing the new capillary Februrary 11, 2013, Rev. B xv

16 Replacing the LockSpray reference probe capillary Removing the existing capillary Installing the new capillary Replacing the NanoLockSpray reference probe capillary Removing the reference probe from the NanoLockSpray source Installing the new TaperTip and capillary Cleaning or replacing the corona pin Replacing the IonSABRE II probe heater Removing the IonSABRE II probe heater Fitting the new IonSABRE II probe heater Replacing the ion block source heater Replacing the LockSpray source s assembly seals Removing the probe adjuster assembly probe and source enclosure seals Fitting the new source enclosure and probe adjuster assembly probe seals Replacing the mass spectrometer s air filters Replacing the air filter inside the front door Replacing the air filters on the sides of the instrument Replacing the IntelliStart Fluidics tubing Replacing IntelliStart Fluidics tubing (Standard configuration) Removing the IntelliStart Fluidics tubing Plumbing the IntelliStart Fluidics lock-spray system Replacing IntelliStart Fluidics tubing (NanoLockSpray) Plumbing the IntelliStart Fluidics sample delivery system Plumbing the IntelliStart Fluidics for low flow sample delivery A Safety Advisories Warning symbols Task-specific hazard warnings Specific warnings xvi Februrary 11, 2013, Rev. B

17 Caution symbol Prohibition symbol Warnings that apply to all Waters instruments and devices Electrical and handling symbols Electrical symbols Handling symbols B External Connections Mass spectrometer external wiring and vacuum connections Connecting the Oerlikon Leybold oil-filled roughing pump Making the electrical connections to the Oerlikon Leybold oil-filled roughing pump Connecting the Edwards oil-free roughing pump Making the electrical connections to the Edwards oil-free roughing pump. 234 Connecting to the nitrogen gas supply Connecting to the collision cell gas supply Connecting the nitrogen exhaust line Connecting liquid waste lines Connecting the workstation (systems with no ACQUITY LC) Connecting Ethernet cables (systems with ACQUITY LC) Input/output signal connectors Signal connections Connecting to the electricity source Connecting the NanoLockSpray source camera Installing the camera driver software Februrary 11, 2013, Rev. B xvii

18 C Materials of Construction and Compatible Solvents Preventing contamination Items exposed to solvent Solvents used to prepare mobile phases xviii Februrary 11, 2013, Rev. B

19 1 Waters Xevo G2-S QTof Overview This chapter describes the instrument, including its controls, sources and IntelliStart Fluidics system. Contents: Topic Page Waters Xevo G2-S QTof LockSpray source and ionization modes NanoLockSpray source and ionization modes Combined APPI/APCI source IntelliStart Fluidics system Ion optics Leak sensors Vacuum system Februrary 11, 2013, Rev. B 19

20 1 Waters Xevo G2-S QTof Overview Waters Xevo G2-S QTof The Xevo G2-S QTof Mass Spectrometry (MS) system is a hybrid, quadrupole, orthogonal acceleration, time-of-flight (TOF) mass spectrometer operated by Waters informatics software. Either of the following high-performance, ZSpray, dual-orthogonal, API sources is fitted as standard equipment: LockSpray (ESI/APCI/ESCi ) source, which combines these ionization modes: Electrospray Atmospheric pressure chemical Combined electrospray and atmospheric pressure chemical See NanoLockSpray source and ionization modes on page 27. NanoLockSpray ESI source, see NanoLockSpray source and ionization modes on page 27. The following optional sources are compatible with the Xevo G2-S QTof:: Combined APPI/APCI source, with the Xevo G2-S QTof (see the Waters APPI Source Operator s Guide Supplement, part number ). Atmospheric Solids Analysis Probe (ASAP) (see the Atmospheric Solids Analysis Probe Operator s Guide Supplement). Atmospheric Pressure Gas Chromatography (APGC) source (see the Atmospheric Pressure GC Operator s Guide Supplement). TRIZAIC UPLC source (see the TRIZAIC UPLC System Guide). For the instrument s specifications, consult the Waters Xevo G2-S QTof Site Preparation Guide (part number ). IntelliStart technology IntelliStart technology monitors instrument performance and reports when it is ready for use. The console software automatically mass calibrates the mass spectrometer and displays performance readbacks to enable simplified setup of the system for use in routine analytical and open access applications. 20 Februrary 11, 2013, Rev. B

21 Waters Xevo G2-S QTof The IntelliStart Fluidics 1 system is built into the mass spectrometer. It delivers sample directly to the MS probe from the LC column or from three integral reservoirs. The reservoirs can also deliver sample through direct or combined infusion so that you can optimize instrument performance at analytical flow rates. An additional reservoir contains solvent for the automated flushing of the solvent delivery system. ACQUITY and nanoacquity Xevo G2-S QTof UPLC/MS systems The Waters Xevo G2-S QTof is compatible with ACQUITY UPLC and nanoacquity UPLC systems, including the H-Class and I-Class systems. If you are not using either system, refer to the documentation specific to your LC system. The ACQUITY Xevo G2-S QTof UPLC /MS system includes an ACQUITY UPLC system and the Waters Xevo G2-S QTof fitted with the LockSpray ESI/APCI/ESCi source. The nanoacquity Xevo G2-S QTof UPLC/MS system includes a nanoacquity UPLC system and the Waters Xevo G2-S QTof fitted with the NanoLockSpray source. ACQUITY UPLC system The ACQUITY UPLC system includes a binary or quaternary solvent manager, sample manager, column heater, sample organizer, detectors, and a specialized ACQUITY UPLC column. Waters informatics software controls the system. For further information, see the ACQUITY UPLC System Operator s Guide or Controlling Contamination in UPLC/MS and HPLC/MS Systems (part number ). You can find the latter document on click Services & Support > Support. 1. In Waters documents, the term fluidics refers to the IntelliStart Fluidics system, the instrument s onboard system that delivers sample and solvent to the probe of the mass spectrometer. It can also denote plumbing components and fluid pathways within and between system modules. Februrary 11, 2013, Rev. B 21

22 1 Waters Xevo G2-S QTof Overview Waters ACQUITY Xevo G2-S QTof UPLC/MS system: Sample organizer (optional) Solvent tray Column heater Access door to the fluidics valves Source interface sliding door Probe High voltage connector for the ESI probe LockSpray source enclosure Xevo G2-S QTof Sample manager Binary solvent manager Access door to the fluidics pump 22 Februrary 11, 2013, Rev. B

23 Waters Xevo G2-S QTof nanoacquity UPLC system The nanoacquity UPLC system includes a binary solvent manager, auxiliary solvent manager, sample manager, column heater, sample organizer, detectors, and a specialized nanoacquity UPLC column. Waters informatics software controls the system. For further information, see the nanoacquity UPLC System Operator s Guide or Controlling Contamination in UPLC/MS and HPLC/MS Systems (part number ). You can find the latter document on click Services & Support > Support. Waters nanoacquity Xevo G2-S QTof UPLC/MS system: Access door to the fluidics valve Access door to the fluidics pump Column heater Solvent tray Source interface sliding door Sample manager NanoLockSpray source enclosure Binary solvent manager Xevo G2-S QTof Februrary 11, 2013, Rev. B 23

24 1 Waters Xevo G2-S QTof Overview Software and data system Waters informatics software controls the mass spectrometer. The software acquires, analyzes, manages, and distributes data from mass spectrometry, ultraviolet (UV), evaporative light scattering, and other sources. Waters informatics software enables these major operations: Configuring the instrument. Creating LC and MS methods that define operating parameters for a run. Tuning and mass calibrating the mass spectrometer. Running samples. Monitoring sample runs. Acquiring data. Processing data. Reviewing data. Printing data. See the online Help for more information. LockSpray source and ionization modes The LockSpray source uses lock-mass correction to acquire exact mass data. The analyte is introduced into the source through a probe. A reference flow containing a compound of known mass flows through a separate ESI probe. An oscillating baffle allows the sprays to be analyzed as two separate data functions. The lock-mass correction calculated from the reference data is then applied to the analyte data set. You can use the LockSpray source with the ESI, APCI, ESCi, and ASAP ionization modes. See Chapter 3 Configuring the LockSpray source. 24 Februrary 11, 2013, Rev. B

25 LockSpray source and ionization modes Xevo G2-S QTof fitted with LockSpray source: Electrospray ionization (ESI) In electrospray ionization (ESI), a strong electrical charge is applied to the eluent as it emerges from a nebulizer. The droplets that compose the resultant aerosol undergo a reduction in size (solvent evaporation). As solvent continues to evaporate, the charge density increases until the droplet surfaces eject ions (ion evaporation). The ions can be singly or multiply charged. To operate the LockSpray source in ESI mode, you fit the source enclosure with an ESI probe. Februrary 11, 2013, Rev. B 25

26 1 Waters Xevo G2-S QTof Overview The standard ESI probe capillary accommodates flow rates of up to 2 ml/min making it suitable for LC applications in the range 100 µl/min to 2 ml/min. To reduce peak broadening for lower-flow-rate LC applications, such as 1-mm UPLC columns, use the optional, small-bore capillary, which can accommodate a maximum flow rate of 200 µl/min. Atmospheric pressure chemical ionization (APCI) Atmospheric pressure chemical ionization (APCI) produces singly-charged protonated or deprotonated molecules for a broad range of nonvolatile analytes. The APCI interface consists of the ESI/APCI/ESCi enclosure fitted with a corona pin and an IonSABRE II probe. Mobile phase from the LC column enters the probe, where it is pneumatically converted to an aerosol, rapidly heated, and vaporized or gasified at the probe tip. APCI mode: IonSABRE II probe Sample cone Corona pin Hot gas from the IonSABRE II probe passes between the sample cone and the corona pin, which is typically operated with a discharge current of 5 µa. Mobile phase molecules rapidly react with ions generated by the corona discharge to produce stable reagent ions. Analyte molecules introduced into the mobile phase react with the reagent ions at atmospheric pressure and typically become protonated (in the positive ion mode) or deprotonated (in the negative ion mode). The sample and reagent ions then pass through the sample cone and into the mass spectrometer. 26 Februrary 11, 2013, Rev. B

27 NanoLockSpray source and ionization modes Combined electrospray/atmospheric pressure chemical ionization (ESCi) In combined electrospray and atmospheric pressure chemical ionization (ESCi ) mode, the standard ESI probe is used in conjunction with a corona pin. The design allows alternating acquisition of ESI and APCI ionization data, facilitating high-throughput processing and wider compound coverage. Atmospheric solids analysis probe (ASAP) The ASAP facilitates rapid analysis of volatile and semivolatile compounds in solids, liquids, and polymers. It is particularly suited to analyzing low-polarity compounds.the ASAP directly replaces the electrospray or IonSABRE II probe in the instrument s source housing and has no external gas or electrical connections. See the Atmospheric Solids Analysis Probe Operator s Guide Supplement for further details. NanoLockSpray source and ionization modes The NanoLockSpray source allows electrospray ionization performed in the flow rate range of 5 to 1000 nl/min. For a given sample concentration, the ion currents for similar experiments approximate those in normal flow rate electrospray. However, because sample consumption is greatly reduced, the sensitivity gains are significant when similar scan parameters are used. Lock-mass correction with the NanoLockSpray source works as the LockSpray source does in electrospray ionization mode. The NanoLockSpray source enclosure consists of a sprayer universal, borosilicate glass capillary, or CE (see below) mounted on a ZSpray three-axis manipulator. A light within the source provides illumination for the spray, which you can observe using the video camera mounted on the corner of the source housing. See Chapter 4 Configuring the NanoLockSpray Source. Februrary 11, 2013, Rev. B 27

28 1 Waters Xevo G2-S QTof Overview Xevo G2-S QTof fitted with NanoLockSpray source: Options shown in the following table are available for the spraying capillary: Spraying-capillary options: Option Universal NanoFlow nebulizer sprayer Borosilicate glass capillary NanoFlow (nanovials) Description For flow injection or coupling to nanoacquity systems. A pump regulates flow rate to as low as 100 nl/min. Uses metal-coated glass capillaries, permitting the lowest flow rates. Usable for one sample only, after which you must discard them. 28 Februrary 11, 2013, Rev. B

29 Combined APPI/APCI source TRIZAIC UPLC source The TRIZAIC UPLC source accepts a nanotile device, which combines the functions of an analytical column, trapping column, and nanospray emitter. This technology simplifies the implementation of capillary-scale chromatography and analysis of limited-volume samples. See the TRIZAIC UPLC System Guide for further details. Combined APPI/APCI source Atmospheric pressure photoionization (APPI) uses photons generated by a discharge UV lamp (~10.2 ev) to produce sample ions from vaporized LC eluent. Direct photoionization of the sample molecule occurs when the photon energy exceeds the ionization potential of the sample molecule. The optional dual-mode (APPI/APCI) ionization source comprises an APPI source enclosure, which is used in conjunction with a standard APCI probe. You can operate the source in APPI or dual mode. The latter mode involves rapid alternation of the APPI and APCI ionization modes, facilitating high-throughput analyses. For further details, see the Waters APPI Source Operator s Guide Supplement (part number ). Februrary 11, 2013, Rev. B 29

30 1 Waters Xevo G2-S QTof Overview IntelliStart Fluidics system The IntelliStart Fluidics system is built into the instrument; it controls how sample is delivered to the source. System connections differ according to whether you are using a LockSpray or NanoLockSpray source. See page 186. For standard flow applications, the system delivers sample directly to the mass spectrometer s source in one of three ways: From the LC column. From three integral reservoir bottles. Use standard reservoir bottles (30 ml) for instrument setup and calibration. Use low-volume vials (1.5 ml) to infuse smaller volumes. The reservoir bottles can also deliver sample through direct or combined infusion, permitting optimization at analytical flow rates. From a wash reservoir that contains solvent for automated flushing of the instrument s solvent delivery system. For nanoacquity, the valves and pumps of the IntelliStart Fluidics system introduce dead volume, which can cause unacceptable peak broadening. For this reason, the nanoacquity is plumbed directly to the NanoFlow sprayer using a suitable, short piece of silica tubing. For reference flows for both the LockSpray and NanoLockSpray source, the IntelliStart Fluidics system delivers lock-mass solution from reservoir bottle B or, for extended operating hours, from a separate, external bottle of lock-mass solution. IntelliStart Fluidics system physical layout The IntelliStart Fluidics system comprises the components shown in the following figure. 30 Februrary 11, 2013, Rev. B

31 IntelliStart Fluidics system IntelliStart Fluidics system components: Lock-spray selector valve Access doors Tube guides Optional Flow sensor Grounded union A B AA B Sample selector valve C C Divert valve A B C Waters A B C Lock-spray pump Sample pump Sample reservoir bottles (A, B and C) Februrary 11, 2013, Rev. B 31

32 1 Waters Xevo G2-S QTof Overview The IntelliStart Fluidics system consists of these components: A sample delivery system composed of a pump, sample selector valve, and a divert valve used for LC and probe connections. A lock-spray system, composed of a pump capable of ultra-low flow rates, a lock-spray selector valve, flow sensor, and grounded union. The grounded union protects the flow sensor from probe voltages. The flow sensor regulates flow rate, reducing it to accommodate the very low volumes required by the NanoLockSpray source. The flow sensor and grounded union are optional fittings when used with the lock-spray system. They are, however, standard fittings when used with the NanoLockSpray source. Three shared 30-mL sample reservoir bottles: A, B, and C. Plumbing for shared wash and waste bottles. The sample reservoirs are mounted on the instrument s front panel. When you select a solvent in the instrument software, a light-emitting diode (LED) illuminates the appropriate reservoir. You can simultaneously illuminate all three reservoirs or extinguish the LEDs, for light-sensitive samples. Recommendation: Use reservoir A for the sample solution, reservoir B for the lock-spray solution, and reservoir C for the calibrant solution. The wash reservoir and (optionally) the reservoirs containing the lock-mass reference solutions are external to the instrument; typically they are bottles on the LC system. The waste reservoir is normally a bottle stored under the instrument bench. During normal operation the instrument access doors must be kept closed. System operation You configure the IntelliStart Fluidics system using the instrument software, in which you can edit parameter settings, frequency, and the extent of the automation. During auto-calibration, the software automatically controls lock-mass and sample delivery. Consult the mass spectrometer s online Help for further details on operating the IntelliStart Fluidics system. 32 Februrary 11, 2013, Rev. B

33 Ion optics Ion optics The mass spectrometer s ion optics operate in the following sequence: 1. Samples from the LC or instrument s solvent delivery system are introduced at atmospheric pressure into the ionization source. 2. The ions pass through the sample cone and into the vacuum system. 3. The ions pass through the StepWave ion guide to the quadrupole, where they are filtered according to their mass-to-charge ratio. 4. The mass-separated ions pass into the T-Wave collision cell, where they can undergo collision-induced dissociation (CID). 5. The ions pass into the time-of-flight (ToF) analyzer. A high voltage pulse orthogonally accelerates the ions up the flight tube, where a reflectron reflects them back towards the detector. Ions of different mass-to-charge ratios arrive at the detector at different times. The difference in the arrival times provides the basis for a mass spectrum. 6. The signal from the detector is amplified, digitized, and transmitted to the software. Ion optics overview: Reflectron Sample sprayer Lock-spray sprayer Quadrupole Flight tube Sample cone DRE lens Isolation valve StepWave ion guide T-Wave collision cell Transfer lenses Pusher Detector Februrary 11, 2013, Rev. B 33

34 1 Waters Xevo G2-S QTof Overview Leak sensors Leak sensors in the instrument s drip trays continuously monitor for liquid leaks. A leak sensor stops system flow when its optical sensor detects about 1.5 ml of accumulated leaked liquid in its surrounding reservoir. At the same time, the software displays an error message alerting you that a leak has developed. Consult the Waters ACQUITY UPLC Leak Sensor maintenance instructions (part number ) for complete details. Vacuum system An external roughing pump and three internal turbomolecular pumps maintain therequired vacuum. Protective interlocks guard against vacuum leaks and electrical or vacuum pump failure. The system monitors the turbomolecular pump speeds and continuously measures vacuum pressure via built-in gauges. The gauges also serve as switches, stopping operation when vacuum loss is sensed. A vacuum isolation valve isolates the source from the mass analyzer, allowing cleaning of the sample cone without the need to vent the instrument to atmospheric pressure. 34 Februrary 11, 2013, Rev. B

35 2 Preparing the Mass Spectrometer for Operation This chapter explains how to start up and shut down the mass spectrometer. Contents: Topic Page Starting the mass spectrometer Preparing the IntelliStart Fluidics system Rebooting the mass spectrometer Leaving the mass spectrometer ready for operation Emergency shutdown of the mass spectrometer Februrary 11, 2013, Rev. B 35

36 2 Preparing the Mass Spectrometer for Operation Starting the mass spectrometer The Waters Xevo G2-S QTof is compatible with the ACQUITY UPLC and nanoacquity UPLC systems. If you are not using either system, refer to the documentation relevant to your LC system (see Software and data system on page 24). Caution: To avoid damage to the instrument caused by incompatible solvents, refer to the following sources: Appendix C, Materials of Construction and Compatible Solvents, for mass spectrometer solvent information. Appendix C of the ACQUITY UPLC System Operator s Guide for solvent compatibility with ACQUITY devices. Requirement: Power-on the instrument server or workstation PC first, to ensure that it can assign IP addresses to LCMS system modules. See the mass spectrometer s online Help for details. To start the mass spectrometer: Warning: To avoid ignition of flammable solvents, never let the nitrogen supply pressure fall below 414 kpa (4.0 bar, 60 psi). 1. On the rear panel, ensure the nitrogen supply is connected to the instrument s nitrogen inlet connection (see the figure on page 226). Requirement: The nitrogen must be dry and oil-free, with a purity of at least 95%. Regulate the supply at 600 to 690 kpa (6.5 to 7.0 bar, 94 to 100 psi). 2. Ensure that the collision gas supply is connected to the instrument s collision cell gas inlet. Requirement: The collision gas is argon; it must be dry and of high purity (99.997%). Regulate the supply at 50 kpa (0.5 bar, 7 psi). 3. Power-on the instrument server or workstation PC. 4. Switch on the Xevo G2-S QTof at the power outlet. 5. Press the power switches of the ACQUITY instruments. Result: Each system component runs a series of startup tests. 36 Februrary 11, 2013, Rev. B

37 Starting the mass spectrometer 6. Allow 4 minutes for the PC to initialize. Tip: The power and status LEDs change as follows: During initialization, the binary solvent manager s and sample manager s status LED flashes green. After the instruments are successfully powered-on, all power LEDs show steady green. The binary solvent manager s flow LED, the sample manager s run LED, and the mass spectrometer s status LED remain unlit. 7. Start the software and monitor the instrument console for messages and LED indications. 8. Click Operate. Result: When the mass spectrometer is in good operating condition, the software indicates Ready in the instrument console. Verifying the instrument s state of readiness When the mass spectrometer is in good operating condition, the power and status LEDs show constant green. You can view any error messages in console software. Monitoring the mass spectrometer LEDs Light-emitting diodes on the mass spectrometer indicate its operational status. Power LED The power LED, below the mass spectrometer s source, indicates when the mass spectrometer is powered-on or powered-off. Status LED The status LED, on the right-hand side of the power LED, indicates the operating condition. See the mass spectrometer s online Help for details on the status LED indications. Calibration Calibrate the mass spectrometer prior to use, see the mass spectrometer s online Help. Februrary 11, 2013, Rev. B 37

38 2 Preparing the Mass Spectrometer for Operation Flow rates for the Xevo G2-S QTof system The Xevo G2-S QTof system can run at high flow rates. To optimize desolvation, and thus sensitivity, run the system at appropriate gas flows and desolvation temperatures. Flow rate versus temperature and gas flow: Flow rate (ml/min) Source temperature ( C) Desolvation temperature ( C) to to to to > Preparing the IntelliStart Fluidics system Desolvation gas flow (L/h) For additional information, see Connecting liquid waste lines on page 239. Warning: To avoid injuries from broken glass, falling objects, or exposure to toxic substances, do not place containers on top of the instrument or on its front covers.instead use the bottle tray. Installing the reservoir bottles Use standard reservoir bottles (30-mL) for instrument setup and calibration. Use the Low-volume Adaptor Kit (included) to infuse smaller volumes. The low-volume vials have a volume of 1.5 ml. Required materials Chemical-resistant, powder-free gloves 38 Februrary 11, 2013, Rev. B

39 Preparing the IntelliStart Fluidics system To install the reservoir bottles: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the reservoir bottles. 1. Remove the reservoir bottle caps. 2. Screw the reservoir bottles onto the mass spectrometer, as shown below. Reservoir bottle Solvent delivery tube 3. For each reservoir bottle, ensure that the ends of the solvent delivery tubes are positioned so that they are close to, but do not touch, the bottom of the bottle (see page 41). Februrary 11, 2013, Rev. B 39

40 2 Preparing the Mass Spectrometer for Operation To install the low-volume vials: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the reservoir bottles. 1. If a standard reservoir bottle is fitted, remove it. 2. Screw the low-volume adaptors into the manifold and finger-tighten them. Low-volume adaptor Low-volume vial Solvent delivery tube Warning: To avoid laceration injuries caused by the shattering of fragile, low-volume glass vials, take care when screwing them in, and never use force. 3. Screw the low-volume vials into the adaptors. 4. For each low-volume vial, ensure that the ends of the solvent delivery tubes are positioned so that they are close to, but do not touch, the bottom of the vial (see page 41). 40 Februrary 11, 2013, Rev. B

41 Preparing the IntelliStart Fluidics system Adjusting the solvent delivery tube positions For correct operation of the IntelliStart Fluidics system, you must adjust each solvent delivery tube so that its end is close to, but does not touch, the bottom of the reservoir bottle or low volume vial. To adjust the position of a solvent delivery tube: 1. Open the access door to the fluidics pump (see the figure on page 30). 2. Loosen the finger-tight fitting for the solvent delivery tube you are adjusting. Finger-tight fitting Solvent delivery tube 3. Move the solvent delivery tube so that its end is close to, but does not touch, the bottom of the reservoir bottle or low volume vial. 4. Tighten the finger-tight fitting. 5. Close the access door. Februrary 11, 2013, Rev. B 41

42 2 Preparing the Mass Spectrometer for Operation Purging the pump Whenever you replace a solution bottle, purge the pump with the solution that you are going to use next. See the mass spectrometer s online Help for details. Requirement: Ensure that the end of the tubing is fully submerged in the solvent in the wash reservoir. Tip: Depending on the solutions used, the system can require more than one purge cycle to minimize carryover. Rebooting the mass spectrometer Reboot the mass spectrometer when either of these conditions applies: The console software fails to initialize or connect. Immediately following a software upgrade. To reboot the mass spectrometer: 1. Open the sliding door above the instruments source enclosure, and locate the reset button aperture. 2. Insert a short length of PEEK tubing into the aperture to press the reset button. Leaving the mass spectrometer ready for operation When you are not using the instrument, stop the LC flow and put the instrument in Standby mode, to conserve energy and reduce nitrogen consumption. Tip: After you return the instrument to Operate mode, the LockSpray source s temperature requires up to 30 minutes to stabilize at the relatively high temperatures needed for UPLC operation. 42 Februrary 11, 2013, Rev. B

43 Emergency shutdown of the mass spectrometer Emergency shutdown of the mass spectrometer To shut down the mass spectrometer in an emergency: Warning: To avoid electric shock, isolate the instrument from the electrical supply, disconnect the power cable from the instrument s rear panel. Note: Data can be lost during an emergency shutdown. 1. Switch off the power at the electrical outlet. Result: The instrument turns off and vents. 2. Disconnect the power cable from the instrument s rear panel. Februrary 11, 2013, Rev. B 43

44 2 Preparing the Mass Spectrometer for Operation 44 Februrary 11, 2013, Rev. B

45 3 Configuring the LockSpray Source This chapter explains how to configure the LockSpray source for the following ionization modes: ESI (electrospray ionization) APCI (atmospheric pressure ionization) ESCi (combined electrospray and atmospheric pressure ionization) Contents: Topic Page Configuring the LockSpray source Configuring for ESI mode Configuring for APCI mode Configuring for ESCi mode Februrary 11, 2013, Rev. B 45

46 3 Configuring the LockSpray Source Configuring the LockSpray source The following table summarizes how you configure the LockSpray source for the various ionization modes. Configuring the LockSpray source: Ionization mode Probe type Corona pin fitted? ESI ESI No APCI APCI Yes ESCi ESI Yes Configuring for ESI mode To operate in ESI mode, you must fit the ESI probe to the LockSpray source enclosure. For more information on using ESI mode, see the Xevo G2-S QTof system online Help. Installing the ESI probe Required materials Chemical-resistant, powder-free gloves Sharp knife or PEEK tubing cutter 46 Februrary 11, 2013, Rev. B

47 Configuring for ESI mode To install the ESI probe: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the LC system connections, ESI probe, and source. Warning: To avoid electric shock, ensure that the instrument is prepared for working on the source before commencing this procedure. 1. Prepare the instrument for working on the source (see page 73). Warning: To avoid puncture wounds, handle the probe with care. 2. Remove the protective sleeve, if fitted, from the ESI probe tip. Februrary 11, 2013, Rev. B 47

48 3 Configuring the LockSpray Source 3. With the probe label facing you, carefully slide the ESI probe into the hole in the probe adjuster assembly, ensuring that the probe location dowel aligns with the location hole in the probe adjuster assembly. Probe label Probe location dowel Location hole of the probe adjuster assembly TP Februrary 11, 2013, Rev. B

49 Configuring for ESI mode ESI probe, mounted on the LockSpray source enclosure: Vernier probe adjuster ESI probe ESI probe cable Probe locking ring High voltage connector Source window Source enclosure release TP03128 Caution: To avoid nitrogen leakage, fully tighten the probe locking ring. 4. Tighten the probe locking ring to secure the probe in place. Tip: An automatic pressure test is performed when the probe is correctly seated in position. 5. Connect the ESI probe s cable to the high voltage connector. 6. Open the access door to the IntelliStart Fluidics system (see the figure on page 31). Warning: To avoid electric shock, do not use stainless steel tubing to connect the diverter valve to the ESI probe; use the PEEK tubing supplied with the instrument. 7. Using a long finger-tight fitting, connect inch ID (or greater) tubing, from port 2 (the top port) of the diverter valve to the ESI probe, Februrary 11, 2013, Rev. B 49

50 3 Configuring the LockSpray Source where you use a PEEK, finger-tight nut and ferrule to connect to the union. Diverter valve Tubing connection ESI probe Probe adjuster assembly Recommendation: To reduce peak broadening, use inch ID tubing for sample flow rates 1.2 ml/min; use inch ID tubing for sample flow rates >1.2 ml/min. Requirements: If you are replacing the tubing supplied with the instrument, you must minimize the length of the tube connecting the diverter valve to the ESI probe. Doing so minimizes delays and dispersion. When cutting the tubing to length, cut it squarely (that is, perpendicular to its horizontal axis). Long finger-tight fitting and PEEK, finger-tight nut and ferrule: Warning: To avoid electric shock, only use natural (beige) PEEK fittings at the top of the probe. 50 Februrary 11, 2013, Rev. B

51 Configuring for ESI mode 8. Close the access door to the IntelliStart Fluidics system. Removing the ESI probe Required materials Chemical-resistant, powder-free gloves To remove the ESI probe: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the LC system connections, ESI probe, and source. Warning: To avoid electric shock, ensure that the instrument is prepared for working on the source before commencing this procedure. 1. Prepare the instrument for working on the source (see page 73). 2. Disconnect the fluidics tubing from the ESI probe. 3. Disconnect the ESI probe s cable from the high voltage connector. 4. Unscrew the probe locking ring. Warning: To avoid puncture wounds, handle the probe with care. 5. Carefully remove the ESI probe from the probe adjuster assembly. 6. If available, fit the protective sleeve to the ESI probe tip. Februrary 11, 2013, Rev. B 51

52 3 Configuring the LockSpray Source Configuring for APCI mode To operate in APCI mode, you must fit the IonSABRE II probe to the LockSpray source enclosure. For more information on using APCI mode, see the Xevo G2-S QTof system online Help. Installing the IonSABRE II probe Required materials Chemical-resistant, powder-free gloves Sharp knife or PEEK tubing cutter To install the IonSABRE II probe: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the LC system connections, IonSABRE II probe, and source. Warning: To avoid electric shock, ensure that the instrument is prepared for working on the source before commencing this procedure. 1. Prepare the instrument for working on the source (see page 73). 2. With the probe label facing toward you, carefully slide the IonSABRE II probe into the hole in the probe adjuster assembly, ensuring that the probe location dowel aligns with the probe adjuster assembly location hole. 52 Februrary 11, 2013, Rev. B

53 Configuring for APCI mode Probe label Probe location dowel Probe adjuster assembly location hole TP Tighten the probe locking ring to secure the probe in place. Tip: An automatic pressure test is performed when the probe is correctly seated in position. Februrary 11, 2013, Rev. B 53

54 3 Configuring the LockSpray Source IonSABRE II probe mounted on the source enclosure: IonSABRE II probe Vernier probe adjuster Vertical probe adjuster Source window Source enclosure release 4. Open the access door to the fluidics valve (see the figure on page 22). Warning: To avoid electric shock, do not use stainless steel tubing to connect the diverter valve to the IonSABRE II probe; use the PEEK tubing supplied with the instrument. 5. Using tubing greater than or equal to inch ID, connect port 2 (the top port) of the diverter valve to the IonSABRE II probe. Recommendation: To reduce peak broadening, use inch ID tubing for sample flow rates 1.2 ml/min; use inch ID tubing for sample flow rates 1.2 ml/min. Requirements: If you are replacing the tubing supplied with the instrument, you must minimize the length of the tube connecting the diverter valve to the ESI probe. Doing so minimizes delays and dispersion. When cutting the tubing to length, cut it squarely (that is, perpendicular to its horizontal axis). 6. Close the access door. 7. Install the corona pin (see page 77). 54 Februrary 11, 2013, Rev. B

55 Configuring for APCI mode Removing the IonSABRE II probe Required materials Chemical-resistant, powder-free gloves To remove the IonSABRE II probe: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the LC system connections, ESI probe, and source. Warning: To avoid electric shock, ensure that the instrument is prepared for working on the source before commencing this procedure. 1. Prepare the instrument for working on the source (see page 73). 2. Remove the corona pin (see page 77). 3. Disconnect the diverter valve tubing from the IonSABRE II probe. 4. Unscrew the probe locking ring. 5. Carefully remove the probe from the probe adjuster assembly. Februrary 11, 2013, Rev. B 55

56 3 Configuring the LockSpray Source Configuring for ESCi mode To operate in ESCi mode, you must fit an ESI probe and corona pin to the LockSpray source enclosure. The system, with the ESI probe installed and corona discharge pin fitted, can alternate between ESI and ESCi modes, facilitating data acquisition in ESI and ESCi modes in parallel. For more information on using dual ESI and ESCi modes, see the Xevo G2-S QTof system online Help. See Installing the ESI probe on page 46, Installing the corona pin in the source on page 77, and Combined electrospray/atmospheric pressure chemical ionization (ESCi) on page 27. Optimizing the ESI probe for ESCi operation See the mass spectrometer s online Help for details on how to optimize the ESI probe for ESCi operation. 56 Februrary 11, 2013, Rev. B

57 4 Configuring the NanoLockSpray Source The Waters NanoLockSpray dual, electrospray, ion source enables the optimized co-introduction of sample and lock mass compound directly into the ion source. This feature provides authenticated, exact-mass measurement in MS mode at low flow rates. Contents: Topic Page Overview of the NanoLockSpray source Selecting and configuring the NanoLockSpray source Deploying the sprayer platform adjuster assembly Adjusting the sprayer tip position Setting up the camera Optional glass capillary sprayer Februrary 11, 2013, Rev. B 57

58 4 Configuring the NanoLockSpray Source Overview of the NanoLockSpray source NanoLockSpray source: LockSpray sprayer inlet Sprayer shield Z-position adjuster Camera Camera focussing ring Sprayer safety cover Shield holding screw X-position adjuster Thumbscrew (on left-hand side of sprayer platform) Sprayer platform adjuster assembly Y-position adjuster Thumbscrew The NanoLockSpray source enclosure holds two nanoflow sprayers positioned orthogonally with respect to one another. The sample flows through one sprayer and the lock-mass reference solution through the other. A motorized baffle rotates to admit spray from either sprayer to the sampling cone. 58 Februrary 11, 2013, Rev. B

59 Overview of the NanoLockSpray source Schematic of the NanoLockSpray source: LockSpray inlet Sample inlet Baffle Sample cone Spray indexing permits acquisition of sample and lock-spray data in separate data channels, and the baffle design ensures negligible cross-talk between the two sprays. The lock-spray data are used to calculate a correction factor for the mass-scale calibration, which is then applied to the sample data, providing exact-mass information. Sample sprayer You can use the NanoLockSpray source with different nanoflow sprayers. For instructions on how to set up these sprayers, see page 60. LockSpray sprayer The LockSpray sprayers for the NanoLockSpray source operate as part of the instrument's IntelliStart Fluidics system. Fitted with a 500 µl pump, the LockSpray sprayer operates at 0.5 µl/min. You must choose the concentration of the lock-spray reference solution that gives a suitable ion intensity. Februrary 11, 2013, Rev. B 59

60 4 Configuring the NanoLockSpray Source NanoFlow gas supply Purge gas The sample sprayer nebulizer gas supply pressure is electronically controlled from 0 to 2 bar. The optimum pressure is sprayer-dependent, but usually lies between 0.3 bar and 1.0 bar. Purge gas typically flows at 100 L/h. It provides a positive pressure in the source enclosure that reduces the chemical background interference caused by contaminants in the laboratory air. For information on adjusting the purge gas flow, see the mass spectrometer s online Help. Sprayer platform adjuster assembly The sprayer platform adjuster assembly allows precise X-, Y-, and Z-positioning of the sprayer tip. You can also withdraw the sprayer from the source to obtain access to the sprayer tip. Using the two thumbscrews on the base of the adjuster assembly, you can move the platform in and out of the source (see Deploying the sprayer platform adjuster assembly on page 61). Selecting and configuring the NanoLockSpray source The Universal NanoFlow sprayer is installed as standard equipment on the NanoLockSpray source. For installation and maintenance details, see the Waters Universal NanoFlow Sprayer Installation and Maintenance Guide (part number ). When fitted, the NanoLockSpray source is automatically recognized by the software. Requirement: The sprayer platform must be inserted in the source enclosure for correct identification of the source. The following table summarizes how you configure the NanoLockSpray source for the various ionization modes. Tip: A corona pin is not used with the NanoLockSpray source. 60 Februrary 11, 2013, Rev. B

61 Deploying the sprayer platform adjuster assembly NanoLockSpray source configuration: Sprayer type Universal NanoFlow sprayer. See Universal NanoFlow Sprayer Installation and Maintenance Guide, (part number ) Borosilicate glass capillary NanoFlow (see page 65). For details, see the Borosilicate Glass Capillary Sprayer Operator s Guide (part number ). Used for For coupling to nanoacquity UPLC with regulated flow rates down to 100 nl/min. Suitable for single shot analyses. This option yields lower flow rates (<30 nl/min) than the Universal Sprayer and hence a smaller sample consumption for a given spectral quality. Deploying the sprayer platform adjuster assembly To move the sprayer platform out of the source: Warning: To avoid electrical shock, ensure the safety cover is in place over the sprayer. 1. Confirm that the sprayer s safety cover is installed (see the figure on page 58). 2. Unscrew the thumbscrew on the front of the sprayer platform. 3. Pull out the side thumbscrew and withdraw the sprayer platform from the source. 4. Release the side thumbscrew, locking the platform in the withdrawn position. Februrary 11, 2013, Rev. B 61

62 4 Configuring the NanoLockSpray Source To move the sprayer platform into the source: Warning: To avoid electrical shock, ensure the safety cover is in place over the sprayer. 1. Confirm the clear sprayer shield is in place and secured (see the figure on page 58). 2. Confirm that the sprayer s safety cover is installed. 3. Pull out the side thumbscrew, and push the sprayer platform into the source. 4. Release the side thumbscrew, locking the platform in position. 5. Tighten the front thumbscrew, securing the adjuster assembly rigidly to the source. 62 Februrary 11, 2013, Rev. B

63 Adjusting the sprayer tip position Adjusting the sprayer tip position To adjust the tip position: 1. Adjust the X, Y, and Z controls on the adjuster assembly to move the sprayer tip close to the sampling cone and baffle. 2. Adjust the height of the sprayer so that its tip is level with the center of the sampling cone, using the index mark on the adjuster. 3. Adjust the horizontal position of the sprayer so that the tip points toward the left-hand side of the baffle. Tips: If you observe an electrical discharge between the sprayer tip and baffle, move the tip farther from the baffle, or reduce the capillary voltage. Note, however, that the capillary voltage must be high enough to maintain a good spray. Fine tune the position of the sprayer while acquiring a spectrum of a standard compound. Small adjustments to the sprayer position can make large differences to the source sensitivity. Februrary 11, 2013, Rev. B 63

64 4 Configuring the NanoLockSpray Source Setting up the camera To set up the camera: 1. Click to open the Camera Control dialog box. Camera Control view of sprayers and sample cone: Sample cone Baffle Sample sprayer Sample spray 2. Rotate the camera s focusing ring to focus on the sample sprayer (see the figure on page 58). 64 Februrary 11, 2013, Rev. B

65 Optional glass capillary sprayer Optional glass capillary sprayer The glass capillary sprayer is designed for use with metal-coated borosilicate glass capillaries. They allow extremely low flow rates (less than 100 nl/min). The glass capillaries are used for one sample only and must then be discarded. To use the glass capillary sprayer, complete the procedures described in the Borosilicate Glass Capillary Sprayer Operator s Guide (part number ). Februrary 11, 2013, Rev. B 65

66 4 Configuring the NanoLockSpray Source 66 Februrary 11, 2013, Rev. B

67 5 Maintenance Procedures This chapter provides the maintenance guidelines and procedures necessary to maintain the instrument s performance. Keep to a maintenance schedule and perform maintenance as required and described in this chapter. Contents: Topic Page Maintenance schedule Spare parts Troubleshooting with Connections INSIGHT Safety and handling Preparing the instrument for working on the source Removing and refitting the source enclosure Installing and removing the corona pin Operating the source isolation valve Removing O-rings and seals Cleaning the instrument case Emptying the nitrogen exhaust trap bottle Maintaining the roughing pump Maintaining the Oerlikon Leybold oil-filled roughing pump Cleaning the source components Cleaning the sampling cone assembly Cleaning the ion block assembly Cleaning the StepWave ion guide assembly Replacing the ESI probe tip and gasket Replacing the ESI probe sample capillary Cleaning the IonSABRE II probe tip Februrary 11, 2013, Rev. B 67

68 5 Maintenance Procedures Contents: (Continued) Topic Page Replacing the IonSABRE II probe sample capillary Replacing the LockSpray reference probe capillary Replacing the NanoLockSpray reference probe capillary Cleaning or replacing the corona pin Replacing the IonSABRE II probe heater Replacing the ion block source heater Replacing the LockSpray source s assembly seals Replacing the mass spectrometer s air filters Replacing the IntelliStart Fluidics tubing Replacing IntelliStart Fluidics tubing (Standard configuration) Replacing IntelliStart Fluidics tubing (NanoLockSpray) Plumbing the IntelliStart Fluidics for low flow sample delivery Februrary 11, 2013, Rev. B

69 Maintenance schedule Maintenance schedule The following table lists periodic maintenance schedules that ensure optimum instrument performance. Maintenance schedule: Procedure Frequency For information... Clean the instrument case. As required. See page 85. Empty the nitrogen exhaust trap bottle. Inspect and adjust the roughing pump s oil level. Replace the roughing pump s oil and oil mist filter. Replace the oil-free (scroll) pump s seals. Clean the source components. Clean the StepWave ion guide. Replace the ESI probe tip. Replace the ESI probe capillary. Clean the IonSABRE II probe tip. (Options using the IonSABRE II probe only.) Check daily, empty as required. See page 85. Weekly. See page 90. Annually. See page 93. Annually. When sensitivity decreases to unacceptable levels. When sensitivity is not improved by cleaning source components. When sensitivity decreases to unacceptable levels. When sensitivity decreases to unacceptable levels or sample flow is inconsistent. When sensitivity decreases to unacceptable levels. See Edwards document XDS35i Instruction Manual A See page 100. See page 125. See page 143. See page 147. See page 155. Februrary 11, 2013, Rev. B 69

70 5 Maintenance Procedures Maintenance schedule: (Continued) Procedure Frequency For information... Replace the IonSABRE II probe capillary. Replace the LockSpray probe capillary. Replace the NanoLockSpray reference probe capillary. Clean or replace the corona pin (APCI and ESCi modes). Replace the IonSABRE II probe heater. Replace the ion block heater cartridge. Replace the source assembly seals. Replace the mass spectrometer air filters. Replace the IntelliStart Fluidics tubing. Spare parts When sensitivity decreases to unacceptable levels or sample flow is inconsistent. See page 155. Annually. See page 162. Annually. See page 165. When the corona pin is corroded or black, or the sensitivity decreases to unacceptable levels. If the heater fails to heat the probe. If the heater fails to heat the ion block. See page 170. See page 171. See page 174. Annually. See page 178. Annually. See page 182. In the event of blockage in the tubing connections between the IntelliStart Fluidics system components. See page 186. Waters recommends that you replace only the parts mentioned in this document. For spare parts details, see the Waters Quality Parts Locator on the Waters Web site s Services & Support page. 70 Februrary 11, 2013, Rev. B

71 Troubleshooting with Connections INSIGHT Troubleshooting with Connections INSIGHT Connections INSIGHT is an intelligent device management (IDM) Web service that enables Waters to provide proactive service and support for the ACQUITY UPLC system. To use Connections INSIGHT, you must install its service agent software on your workstation. In a client/server system, the service agent must also be installed on the computer from which you control the system. The service agent software automatically and securely captures and sends information about the support needs of your system directly to Waters. If you encounter a performance issue when using the Instrument Console, you can manually submit a Connections INSIGHT request to Waters customer support. Alternatively, you can use Remote Desktop, a real-time collaboration option that controls the two-way connection with the ACQUITY UPLC system by enabling the Connections INSIGHT iassist service level. Consult these sources for more information about Connections INSIGHT and Connections INSIGHT iassist: Connections INSIGHT Installation Guide (part number ) Connections INSIGHT User's Guide (part number ) Your sales representative Your local Waters subsidiary Waters Customer Support To submit a Connections INSIGHT request: 1. Select Troubleshoot > Submit Connections INSIGHT request. 2. In the Connections INSIGHT Request dialog box, type your name, telephone number, address, and a description of the problem. 3. Click Submit and allow approximately 5 minutes to save the service profile. Result: A.zip file containing your Connections INSIGHT profile is forwarded to Waters customer support for review. Saving a service profile or plot file from the Instrument Console can require as much as 150 MB of file space. Februrary 11, 2013, Rev. B 71

72 5 Maintenance Procedures Safety and handling Bear in mind the following safety considerations when performing maintenance procedures: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the LC system connections, ESI probe, and source components. Warning: To prevent injury, always observe Good Laboratory Practice when handling solvents, changing tubing, or operating the instrument. Know the physical and chemical properties of the solvents used (see the Material Safety Data Sheets for the solvents in use). Warning: To avoid electric shock, do not remove the instrument s panels. There are no user-serviceable items inside the instrument. ensure that the instrument is in Standby mode before commencing any maintenance. Warning: To avoid burn injuries, take great care when working with the probe and source. Warning: To avoid puncture wounds, take great care while working with the source enclosure open when one or both of these conditions apply: An ESI probe is fitted (the probe tip is sharp). A corona pin is fitted (the pin s tip is sharp). See Appendix A for safety advisory information. 72 Februrary 11, 2013, Rev. B

73 Preparing the instrument for working on the source Preparing the instrument for working on the source For safety reasons, you must follow the procedure described below before working on the source (for example, when changing the probe, installing or removing the corona pin, or operating the source isolation valve) and when maintaining the source. To prepare the instrument for working on the source: 1. In the instrument console, stop the LC flow or, if column flow is required, divert the LC flow to waste. 2. In the instrument console, select source standby and confirm that the operate indicator is not illuminated. 3. Set the source temperature to 30 C. 4. Wait for the source temperature, desolvation temperature or IonSABRE II probe temperature to cool. 5. Ensure that the API desolvation gas flow is stopped. Removing and refitting the source enclosure Before performing certain maintenance procedures, or fitting the optional dual-mode APPI/APCI source to the instrument, you must remove the LockSpray or NanoLockSpray source enclosure from the instrument. Note: The following procedures apply to both the standard and optional source enclosures. Removing the source enclosure from the instrument Required materials Warning: To avoid burn injuries, take great care when working on the source and probe, as they can be hot. Chemical-resistant, powder-free gloves Februrary 11, 2013, Rev. B 73

74 5 Maintenance Procedures To remove the source enclosure: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the LC system connections, ESI probe, and source components. 1. Prepare the instrument for working on the source (see page 73). Warning: To avoid burn injuries, take great care while working with the probe and source. 2. Remove the probe from the source: If you are removing an ESI probe, see page 51. If you are removing an IonSABRE II probe, see page Slide open the instrument s source interface door (see the figure on page 22). 4. Disconnect the probe adjuster and options cables from the instrument s connectors. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if a corona pin is fitted. The pin s tip is sharp. Caution: To avoid damaging the sample inlet, when removing a NanoLockSpray source enclosure, you must slide the sprayer platform out of the source enclosure before you open the enclosure (see page 61). 5. Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open the enclosure. 74 Februrary 11, 2013, Rev. B

75 Removing and refitting the source enclosure 6. Using both hands, grasp the source enclosure, and lift it vertically off the two supporting studs on the source adaptor housing. Cable storage positions Supporting stud TP03164 Source enclosure 7. Store the cables neatly by plugging them into the cable-storage positions on the rear of the source enclosure. Februrary 11, 2013, Rev. B 75

76 5 Maintenance Procedures Fitting the source enclosure to the instrument Required materials Chemical-resistant, powder-free gloves To fit the source enclosure to the instrument: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the LC system connections, ESI probe, and source components. Warning: To avoid puncture wounds, take great care while fitting the source enclosure to the source if a corona pin is fitted. The pin s tip is sharp. 1. Using both hands, fit the source enclosure to the two supporting studs on the source adaptor housing. Caution: To avoid damaging the sample inlet, when removing a NanoLockSpray source enclosure, you must slide the sprayer platform out of the source enclosure before you open the enclosure (see page 61). 2. Close the source enclosure. 3. Connect the probe adjuster and options cables to the instrument s connectors. Tip: The cables and connectors are color coded; the blue-sleeved cable connects to the blue connector and the yellow-sleeved cable to the yellow connector. 4. Slide closed the instrument s source interface door. 76 Februrary 11, 2013, Rev. B

77 Installing and removing the corona pin Installing and removing the corona pin For APCI, ESCi, and dual-mode APPI/APCI operation, you must fit a corona pin to the source. Installing the corona pin in the source Required materials Chemical-resistant, powder-free gloves To install the corona pin in the source: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the LC system connections, ESI probe, and source components. Warning: To avoid electric shock,prepare the instrument for work performed on its source before beginning this procedure. 1. Prepare the instrument for working on the source (see page 73). Warning: To avoid burn injuries, take great care while working with the source enclosure open. Warning: To avoid puncture wounds, take great care while working with the source enclosure open when an ESI probe is fitted. The probe s tip is sharp. 2. Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open the enclosure. 3. Remove the blanking plug from the corona pin mounting contact. Tip: Store the blanking plug in a safe location. Februrary 11, 2013, Rev. B 77

78 5 Maintenance Procedures Corona pin mounting contact: Corona pin mounting contact blanking plug TP03130 Warning: To avoid puncture injury, handle the corona pin with care. Its tip is sharp. 4. Fit the corona pin to the corona pin mounting contact, ensuring that the corona pin is securely mounted and that its tip aligns with the sample cone orifice. 78 Februrary 11, 2013, Rev. B

79 Installing and removing the corona pin Corona pin: Corona pin Sample cone orifice 5. Close the source enclosure. TP Look through the source window, and use the vernier probe adjuster (see page 47) to position the ESI probe tip so that it is pointing approximately midway between the tips of the sample cone and the corona pin. Februrary 11, 2013, Rev. B 79

80 5 Maintenance Procedures Removing the corona pin from the source Required materials Chemical-resistant, powder-free gloves To remove the corona pin from the source: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the LC system connections, ESI probe, and source components. Warning: To avoid electric shock,prepare the instrument for work performed on its source before beginning this procedure. 1. Prepare the instrument for working on the source (see page 73). Warning: To avoid burn injuries, take great care while working with the instrument s source enclosure open. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if an ESI probe is fitted. The probe s tip is sharp. 2. Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open the enclosure. 3. Remove the corona pin from its mounting contact (see the figure on page 79). Tip: Store the corona pin in a safe location. 4. Fit the blanking plug to the corona pin mounting contact (see the figure on page 78). 5. Close the source enclosure. 80 Februrary 11, 2013, Rev. B

81 Operating the source isolation valve Operating the source isolation valve You must close the source isolation valve to isolate the source from the instrument vacuum system for certain maintenance procedures. Required materials Chemical-resistant, powder-free gloves To close the source isolation valve before starting a maintenance procedure: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. 1. Prepare the instrument for working on the source (see page 73). Warning: To avoid burn injuries, take great care working with the instrument s source enclosure open. Warning: To avoid puncture wounds, take great care working with the source enclosure open if one or both of these conditions apply: An ESI probe is fitted (the probe s tip is sharp). A corona pin is fitted (the pin s tip is sharp). 2. Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open the enclosure. Februrary 11, 2013, Rev. B 81

82 5 Maintenance Procedures 3. Close the source isolation valve by moving its handle counterclockwise, to the vertical position. Isolation valve handle in closed position TP Februrary 11, 2013, Rev. B

83 Operating the source isolation valve To open the source isolation valve after completing a maintenance procedure: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if one or both of these conditions apply: An ESI probe is fitted (the probe s tip is sharp). A corona pin is fitted (the pin s tip is sharp). 1. Open the source isolation valve by moving its handle clockwise to the horizontal position. Isolation valve handle in open position TP Close the source enclosure. Februrary 11, 2013, Rev. B 83

84 5 Maintenance Procedures Removing O-rings and seals When performing certain maintenance procedures, you must remove O-rings or seals from instrument components. An O-ring removal kit is provided with the instrument. O-ring removal kit: Tool 1 Tool 2 To remove an O-ring: Caution: To avoid damaging the component when removing an O-ring or seal from it, emsure that you do not scratch the component with the removal tool. Use the tools as aids to pull the O-ring or seal from its groove. Tip: If the O-ring or seal is not going to be reused, you can use the forked end of tool 1 to impale the O-ring or seal, aiding its removal. 84 Februrary 11, 2013, Rev. B

85 Cleaning the instrument case Cleaning the instrument case Caution: To avoid abrading the surfaces of the instrument, do not use abrasives or solvents when cleaning them. Use a soft cloth, dampened with water, to clean the outside surfaces of the mass spectrometer. Emptying the nitrogen exhaust trap bottle Inspect the nitrogen exhaust trap bottle in the instrument exhaust line daily, and empty it before it is more than approximately 10% full. Nitrogen exhaust trap bottle: To laboratory exhaust port From instrument exhaust connection Cap From instrument pilot valve port Nitrogen exhaust trap bottle Februrary 11, 2013, Rev. B 85

86 5 Maintenance Procedures Required materials Chemical-resistant, powder-free gloves To empty the nitrogen exhaust trap bottle: 1. Click Stop Flow, as shown below, according to your software: Software Action MassLynx In the instrument console, click Stop Flow. UNIFI On the System Console tool bar, click Stop Flow. 2. Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open the enclosure. Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when handling waste liquid that accumulates in the nitrogen exhaust trap. 3. Unscrew and remove the nitrogen exhaust trap bottle from the cap and associated fittings. Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of waste liquid according to local environmental regulations. 4. Dispose of the waste liquid in accordance with local environmental regulations. 5. Fit and tighten the nitrogen exhaust trap bottle to the cap. 6. Secure the nitrogen exhaust trap bottle in the upright position. 7. Close the source enclosure. Tip: An automatic pressure test will now be performed. 86 Februrary 11, 2013, Rev. B

87 Emptying the nitrogen exhaust trap bottle 8. Click Start Flow, as shown below, according to your software: Software Action MassLynx In the instrument console, click Start Flow. UNIFI On the System Console tool bar, click Start Flow. Februrary 11, 2013, Rev. B 87

88 5 Maintenance Procedures Maintaining the roughing pump You can use the mass spectrometer with either an Oerlikon Leybold oil-filled roughing pump, or an Edwards XDS46i oil-free roughing pump. For information on connecting the Oerlikon Leybold roughing pump to the mass spectrometer, see page 227. For information connecting the Edwards XDS46i roughing pump to the mass spectrometer, see page 231. Maintaining the Oerlikon Leybold oil-filled roughing pump Requirement: In addition to the roughing pump maintenance requirements detailed here, refer to the manufacturer s documentation provided with the instrument. 88 Februrary 11, 2013, Rev. B

89 Maintaining the Oerlikon Leybold oil-filled roughing pump Oerlikon Leybold roughing pump: Oil filler plug Oil level sight glass Oil drain plug Gas ballast valve TP03296 Februrary 11, 2013, Rev. B 89

90 5 Maintenance Procedures Gas ballasting the Oerlikon Leybold roughing pump Caution: To avoid shortening oil life and, consequently, the useful life of the roughing pump, routinely gas ballast the pump. The roughing pump draws large quantities of solvent vapors that condense in the pump oil, diminishing pumping efficiency. Gas ballasting purges condensed contaminants from the oil. Gas ballast the roughing pump when these conditions apply: With ESI operation, once a week. When the roughing pump oil appears cloudy. When the vacuum pressure is higher than normal. When condensate forms in the roughing pump exhaust line. When you change the roughing pump oil. To gas ballast the roughing pumps: Warning: To avoid burn injuries, take great care while working with the roughing pump. Caution: To avoid damage, do not vent the instrument when the roughing pump is gas ballasting. do not gas ballast the roughing pump while the instrument is in Operate mode. avoid gas ballasting the roughing pump for more than two hours. 1. Open the gas ballast valve. 2. Allow the pump to operate for 30 to 60 minutes. Tip: It is normal for the roughing pump temperature to increase during ballasting. To maintain an ambient temperature of 40 C (104 F) where the pump is located, ensure there is adequate ventilation. 3. Close the gas ballast valve. Inspecting the roughing pump oil level This procedure does not apply to an Edwards oil-free roughing pump. 90 Februrary 11, 2013, Rev. B

91 Maintaining the Oerlikon Leybold oil-filled roughing pump To ensure correct operation of the roughing pump, do not operate it with the oil level at less than 30% of the maximum level, as indicated in the pump s sight glass. Requirement: You must determine the oil level while the roughing pump is running. For each roughing pump, the oil level appears in the roughing pump s oil level sight glass. Check the oil level weekly; you must maintain the oil level at or near the indicated maximum level when the pump is not operating. Tip: The oil level in the sight glass is lower when the roughing pump is running than when it is stopped. When the pump is running, the oil level is typically at 30% to 60% of the maximum level. Adding oil to the roughing pump If the roughing pump s oil level is low (see page 90), you must add oil the pump. Required materials Chemical-resistant, powder-free gloves 12 mm Allen wrench 6 mm Allen wrench Funnel Anderol vacuum oil, type GD32 To add oil to the roughing pump: 1. Vent and shut down the mass spectrometer (see the mass spectrometer s online Help for details). Warning: To avoid personal injury, as well as damage to the roughing pump and mass spectrometer, disconnect the power cords for the mass spectrometer and roughing pump from the main power source. 2. Disconnect the power cords for the mass spectrometer and roughing pump from the main power source. Februrary 11, 2013, Rev. B 91

92 5 Maintenance Procedures 3. Allow the oil to settle in the pump. Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when peforming this procedure. Warning: To avoid burn injuries, take great care while working with the roughing pump: it can be hot. 4. Use the 12 mm Allen wrench to unscrew and remove the pump s oil filler plug. 5. Using the funnel, add Anderol vacuum oil, type GD32, into the oil filler aperture until the oil reaches the maximum level ( MAX ), as indicated in the pump s oil level sight glass. Requirement: Use only Anderol vacuum oil, type GD32. Caution: To avoid oil leakage when fitting the oil filler plug to the roughing pump: inspect the O-ring on the plug, and verify that it is free of particles. ensure that the plug is not cross threaded. do not overtighten the plug. 6. Use the 12 mm Allen wrench to refit the oil filler plug. Tip: When the plug is tightened, it seals against an O-ring. Compression is controlled by the O-ring groove depth in the plug. Increased torque does not improve the plug seal; it only makes the plug difficult to remove later. 7. Connect the power cords for the mass spectrometer and the roughing pump to the main power source. 8. Start the mass spectrometer (see page 36). Tips: After you add oil to the pump, the following situations can occur: The oil level drops slightly during the first month of operation. The oil changes color (darkens) over time. 92 Februrary 11, 2013, Rev. B

93 Maintaining the Oerlikon Leybold oil-filled roughing pump After running the pump for 12 to 48 hours, it is common to see a few drops of oil near the filler plug. Excess oil around the lip of the filler plug will run down and drip off the pump once the pump reaches operating temperature. When the pump begins to run at normal operating temperature, spilled oil becomes slightly odorous. Replacing the roughing pump s oil and oil demister elements Replace the roughing pump s oil and oil demister elements annually. Note: This procedure is not required for an Edwards oil-free roughing pump. Required materials Chemical-resistant, powder-free gloves 12 mm Allen wrench 10 mm wrench Container to catch used oil Funnel Anderol vacuum oil, type GD32 To prepare for replacing the roughing pumps oil and oil demister elements: 1. Gas ballast the roughing pump for 1 hour (see page 90). Rationale: Gas ballasting helps circulate and mix the oil through the pump before draining. 2. Vent and shut down the mass spectrometer (see the mass spectrometer s online Help for details). Warning: To avoid personal injury, as well as damage to the roughing pump and mass spectrometer, disconnect the power cords for the mass spectrometer and roughing pumps from the main power source. 3. Disconnect the power cords for the mass spectrometer and the roughing pump from the main power source. 4. Allow the roughing pump to cool. Februrary 11, 2013, Rev. B 93

94 5 Maintenance Procedures To drain the roughing pump s oil: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when peforming this procedure. Warning: To avoid burn injuries, take great care while working with the roughing pump: it can be hot. 1. Place the container for catching used oil under the pump s drain plug (see the figure on page 88). 2. Use the 12 mm Allen wrench to unscrew and remove the roughing pump s oil filler plug. 3. Use the 12 mm Allen wrench to remove the oil drain plug. 4. Tip the pump toward the drain plug aperture, and allow the oil to drain completely into the container. Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of waste liquid according to local environmental regulations. 5. Dispose of the roughing pump oil in accordance with local environmental regulations. 6. Ensure that the O-ring on the oil drain plug is clean and properly seated. Caution: To avoid oil leakage when fitting the oil drain plug to the roughing pump: ensure that the plug is not cross threaded. ensure that the O-ring is not pinched. do not overtighten the plug. 7. Use the 12 mm Allen wrench to refit the oil drain plug. Tip: When the plug is tightened, it seals against an O-ring. Compression is controlled by the O-ring groove depth in the plug. Increased torque 94 Februrary 11, 2013, Rev. B

95 Maintaining the Oerlikon Leybold oil-filled roughing pump does not improve the plug seal; it only makes the plug difficult to remove later. To fill the roughing pump with oil: 1. Using the funnel, pour one liter of Anderol vacuum oil, type GD32, into the oil filler aperture. Requirement: Use only Anderol vacuum oil, type GD32. Tip: After you add the oil, the level appearing in the sight glass can be above the maximum indication. This apparent excess does not suggest a problem. During the first few days of operation, the oil level falls to within the normal operating range. 2. Ensure that the O-ring on the oil filler plug is clean and properly seated. Caution: To avoid oil leakage when fitting the oil filler plug to the roughing pump: ensure that the plug is not cross threaded. ensure that the O-ring is not pinched. do not over tighten the plug. 3. Use the 12 mm Allen wrench to refit the oil filler plug. Tip: When the plug is tightened, it seals against an O-ring. Compression is controlled by the O-ring groove depth in the plug. Increased torque does not improve the plug seal; it only makes the plug difficult to remove later. Februrary 11, 2013, Rev. B 95

96 5 Maintenance Procedures To remove the roughing pump s oil demister element: 1. Use the 6 mm Allen wrench to remove the four bolts securing the exhaust flange to the roughing pump. Securing bolt Exhaust flange TP Using both hands, carefully remove the exhaust flange and oil demister element from the roughing pump. Oil demister element TP Februrary 11, 2013, Rev. B

97 Maintaining the Oerlikon Leybold oil-filled roughing pump 3. Use the 10 mm wrench to remove the nut that secures the oil demister element to the exhaust flange. Spring Securing nut TP Holding the oil demister element slightly elevated, to prevent the loss of the spring, remove its flange. TP02692 Februrary 11, 2013, Rev. B 97

98 5 Maintenance Procedures 5. Remove the spring from the oil demister element. 6. Dispose of the oil demister element in accordance with local environmental regulations. To fit the new oil demister element: Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the oil demister element according to local environmental regulations. Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when peforming this procedure. 1. Fit the spring to the new oil demister element. TP Holding the oil demister element slightly elevated, to prevent the loss of the spring, fit its exhaust flange. Requirement: Do not overtighten the nut that secures the oil demister element to the exhaust flange. Ensure that only (approximately) 1 mm of exposed thread appears beyond the nut when it is tightened. 98 Februrary 11, 2013, Rev. B

99 Maintaining the Oerlikon Leybold oil-filled roughing pump 3. Use the 10 mm wrench to fit and tighten the nut that secures the oil demister element to the exhaust flange. TP mm exposed thread after tightening 4. Ensure that the inscription TOP is at the top of the oil demister element, and, using both hands, carefully fit the oil demister element and exhaust flange to the roughing pump. 5. Use the 6 mm Allen wrench to fit the four bolts securing the exhaust flange to the roughing pump. Requirement: Tighten the bolts securing the source exhaust flange to the roughing pump sequentially and by small increments until they are all fully tight. To prepare for operation after changing the roughing pump s oil and oil demister element: 1. Connect the power cords for the mass spectrometer and the roughing pump to the main power source. 2. Start the mass spectrometer (see page 36). 3. Gas-ballast the roughing pump (see page 90). Tip: After you add oil to the pump, the following situations can occur: The oil level drops slightly during the first month of operation. The oil changes color (darkens) over time. Februrary 11, 2013, Rev. B 99

100 5 Maintenance Procedures After running the pump for 12 to 48 hours, a few drops of oil sometimes appear near the filler plug. Excess oil around the lip of the filler plug flows downward and drips from the pump when it reaches operating temperature. When the pump approaches operating temperature, any oil spilled becomes slightly odorous. Cleaning the source components Clean the sample cone and cone gas nozzle when these conditions apply: The sample cone and cone gas nozzle are visibly fouled. LC and sample-related causes for decreased signal intensity have been dismissed. See Cleaning the sampling cone assembly on page 101. If cleaning the sample cone and cone gas nozzle fails to increase signal sensitivity, clean the ion block and isolation valve (see page 106). If cleaning the ion block and isolation valve fails to increase signal sensitivity, also clean the Stepwave assembly (see page 125). 100 Februrary 11, 2013, Rev. B

101 Cleaning the sampling cone assembly Cleaning the sampling cone assembly You can remove the sampling cone assembly (comprising the sample cone, O-ring, and cone gas nozzle) for cleaning without venting the instrument. Removing the sampling cone assembly from the source Required materials Chemical-resistant, powder-free gloves To remove the sampling cone assembly from the source: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when peforming this procedure. Warning: To avoid electric shock, ensure that the instrument is in Standby mode before commencing this procedure. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if one or both of these conditions apply: An ESI probe is fitted (the probe tip is sharp). A corona pin is fitted (the pin tip is sharp). Warning: The source can be hot. To avoid burn injuries, take great care while working with the source enclosure open. 1. Close the source isolation valve (see page 81). Februrary 11, 2013, Rev. B 101

102 5 Maintenance Procedures 2. Grasp the cone gas nozzle handle, and use it to rotate the sampling cone assembly 90 degrees, moving the handle from the vertical to horizontal position. Sampling cone assembly Cone gas nozzle handle TP Slide the sampling cone assembly out of the ion block assembly. Ion block assembly TP03132 Important: Do not open the isolation valve at any time when the sampling cone assembly has been removed from the ion block assembly. 102 Februrary 11, 2013, Rev. B

103 Cleaning the sampling cone assembly Disassembling the sampling cone assembly Required materials Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool To disassemble the sampling cone assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when handling the sampling cone assembly. 1. Retrieve the combined 2.5-mm Allen wrench and cone extraction tool from its storage location behind the source enclosure. Combined 2.5-mm Allen wrench and cone extraction tool TP03130 Februrary 11, 2013, Rev. B 103

104 5 Maintenance Procedures 2. Slide the collar to the end of the tool. Collar 3. Insert the collar in the sample cone. Caution: To avoid damaging the sampling cone, which is fragile, do not place it on its tip; always place it on its flanged base. 104 Februrary 11, 2013, Rev. B

105 Cleaning the sampling cone assembly 4. Rotate and lift the tool and collar to remove the sample cone from the cone gas nozzle. 5. Remove the O-ring from the sample cone. Cone gas nozzle Sample cone Cone gas nozzle handle O-ring Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the O-ring according to local environmental regulations. 6. If the O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. 7. Unscrew and remove the PEEK cone gas nozzle handle from the cone gas nozzle. Februrary 11, 2013, Rev. B 105

106 5 Maintenance Procedures Cleaning the sample cone and cone gas nozzle Required materials Chemical-resistant, powder-free gloves. Appropriately sized glass vessels in which to completely immerse components when cleaning. Use only glassware not previously cleaned with surfactants. HPLC-grade (or better) methanol. HPLC-grade (or better) water. Formic acid. Ultrasonic bath. Source of oil-free, inert gas (nitrogen or argon) for drying (air-drying optional). Wash bottle containing HPLC-grade (or better) 1:1 methanol/water. Large beaker. To clean the sample cone and cone gas nozzle: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the sample cone and cone gas nozzle. Warning: To avoid injury when working with formic acid, which is extremely corrosive and toxic, take extreme care handling it, and use a fume hood and suitable protective equipment. Caution: The sample cone is fragile. Never place it on its tip; always place it on its flanged base. 106 Februrary 11, 2013, Rev. B

107 Cleaning the sampling cone assembly 1. If the sample cone contains debris, place a drop of formic acid on its orifice. 2. Immerse the sample cone, cone gas nozzle, and cone gas nozzle handle in separate glass vessels containing 1:1 methanol/water. Tip: If the components are obviously contaminated, use 45:45:10 methanol/water/formic acid. 3. Place the vessels in the ultrasonic bath for 30 minutes. 4. If you used formic acid in the cleaning solution, do as follows: a. Rinse the components by immersing them in separate glass vessels containing water and then placing the vessels in the ultrasonic bath for 20 minutes. b. Remove any residual water from the components by immersing them in separate glass vessels containing methanol and then placing the vessels in the ultrasonic bath for 10 minutes. Caution: To avoid recontaminating the components, wear clean, chemical-resistant, powder-free gloves for the rest of this procedure. 5. Carefully remove the components from the vessels, and blow dry them with inert, oil-free gas. 6. Inspect each component for persisting contamination. If contamination is present, do as follows: a. Use the wash bottle containing 1:1 methanol/water to rinse the component over the large beaker. b. Blow dry the component with inert, oil-free gas. Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of components according to local environmental regulations. 7. Inspect each component for persisting contamination. Requirement: If contamination is present, clean the component again. If contamination is still present, dispose of the component, according to local environmental regulations, and obtain a new one before reassembling the sampling cone assembly. Februrary 11, 2013, Rev. B 107

108 5 Maintenance Procedures Assembling the sampling cone assembly Required materials Chemical-resistant, powder-free gloves To assemble the sampling cone assembly: Caution: To avoid recontaminating the sampling cone assembly, wear clean chemical-resistant, powder-free gloves during this procedure. The sample cone is fragile. Never place it on its tip; always place it on its flanged base. 1. Fit the cone gas nozzle handle to the cone gas nozzle and turn the handle clockwise to tighten. O-ring Sample cone Cone gas nozzle Cone gas nozzle handle TP Carefully fit the sample cone into the cone gas nozzle. 3. Fit the O-ring (a new one, if you disposed of the old O-ring) into the groove created between the sample cone and cone gas nozzle. Fitting the sampling cone assembly to the source Required materials Chemical-resistant, powder-free gloves 108 Februrary 11, 2013, Rev. B

109 Cleaning the sampling cone assembly To fit the sampling cone assembly to the source: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if one or both of these conditions apply: An ESI probe is fitted (the probe tip is sharp). A corona pin is fitted (the pin tip is sharp). Caution: To avoid damage, do not open the source isolation valve before fitting the sampling cone assembly to the ion block assembly. Februrary 11, 2013, Rev. B 109

110 5 Maintenance Procedures 1. Ensure that the source isolation valve is in the closed position (see page 81). 2. Hold the sampling cone assembly so that the cone gas nozzle handle is oriented horizontally and at the top, and then slide the sampling cone assembly into the ion block assembly. Ion block assembly 3. Grasp the cone gas nozzle handle, and use it to rotate the sampling cone assembly 90 degrees, moving the handle downward from the horizontal to the vertical position. 4. Open the source isolation valve (see page 83). 5. Close the source enclosure. TP03132 Sampling cone assembly 110 Februrary 11, 2013, Rev. B

111 Cleaning the ion block assembly Cleaning the ion block assembly Clean the ion block assembly if cleaning the sample cone and cone gas nozzle fails to increase signal sensitivity. Removing the ion block assembly from the source assembly Required materials Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool To remove the ion block assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. 1. Vent and shut down the mass spectrometer (see the mass spectrometer s online Help for details). Warning: To avoid personal injury, as well as damage to the roughing pumps and mass spectrometer, disconnect the power cords for the mass spectrometer and roughing pumps from the main power source. 2. Disconnect the power cords for the mass spectrometer and the roughing pump from the main power source. Warning: To avoid burn injuries, allow a hot source to cool for at least 30 minutes before proceeding. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if one or both of these conditions apply: An ESI probe is fitted (the probe tip is sharp). A corona pin is fitted (the pin tip is sharp). Februrary 11, 2013, Rev. B 111

112 5 Maintenance Procedures 3. Remove the source enclosure (see page 73). Rationale: Removing the source enclosure aids access to the ion block assembly. 4. Close the source isolation valve (see page 81). 5. Use the combined 2.5-mm Allen wrench and cone extraction tool to unscrew the 4, captive, ion block assembly securing screws. Ion block assembly securing screws TP Februrary 11, 2013, Rev. B

113 Cleaning the ion block assembly 6. Remove the ion block assembly from the PEEK ion block support. PEEK ion block support Ion block assembly TP03131 Februrary 11, 2013, Rev. B 113

114 5 Maintenance Procedures Disassembling the source ion block assembly Required materials Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool O-ring removal kit To disassemble the ion block assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. 1. Ensure that the source isolation valve is closed. Source isolation valve handle in closed position Sampling cone assembly retaining blocks Cone gas nozzle handle 2. Grasp the cone gas nozzle handle, and use it to rotate the sampling cone assembly through 90 degrees. 3. Slide the sampling cone assembly out of the ion block assembly. 4. Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate. 114 Februrary 11, 2013, Rev. B

115 Cleaning the ion block assembly Important: To ensure correct operation of the ion block assembly after reassembly, do not remove the sampling cone assembly retaining blocks. do not adjust the screws securing the sampling cone assembly retaining blocks. Ion block cover plate securing screw Ion block cover plate 5. Remove the ion block cover plate. Februrary 11, 2013, Rev. B 115

116 5 Maintenance Procedures 6. Grasp the isolation valve, and pull it out of the ion block. Isolation valve O-ring 7. Use the O-ring removal kit to carefully remove the isolation valve O-ring (see page 84). Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the isolation valve O-ring according to local environmental regulations. 8. If the isolation valve O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. 9. Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw. 116 Februrary 11, 2013, Rev. B

117 Cleaning the ion block assembly Heater cartridge assembly wires PEEK terminal block securing screw Februrary 11, 2013, Rev. B 117

118 5 Maintenance Procedures Caution: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when removing the assembly and ceramic heater mounting block from the ion block. 10. Carefully remove the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, from the ion block. Tip: You can invert the ion block assembly to facilitate this process. PEEK terminal block Ceramic heater mounting block 118 Februrary 11, 2013, Rev. B

119 Cleaning the ion block assembly 11. Use the O-ring removal kit to carefully remove the cover seal from the ion block (see also Removing O-rings and seals on page 84). Cover seal Cone gas O-ring 12. Use the O-ring removal kit to carefully remove the cone gas O-ring from the ion block. Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the cover seal and cone gas O-ring according to local environmental regulations. 13. If the cover seal or cone gas O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. Februrary 11, 2013, Rev. B 119

120 5 Maintenance Procedures Cleaning the ion block components Required materials Chemical-resistant, powder-free gloves. Appropriately sized glass vessels in which to completely immerse components when cleaning. Use only glassware not previously cleaned with surfactants. HPLC-grade (or better) methanol. HPLC-grade (or better) water. Formic acid. Ultrasonic bath. Source of oil-free, inert gas (for example, nitrogen) for drying (air-drying optional). Wash bottle containing HPLC-grade (or better) 1:1 methanol/water. Large beaker. To clean the ion block components: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the ion block components. Warning: To avoid injury when working with formic acid, which is extremely corrosive and toxic, take extreme care handling it, and use a fume hood and suitable protective equipment. 1. Immerse the ion block and isolation valve in separate glass vessels containing 1:1 methanol/water. Tip: If the components are obviously contaminated, use 45:45:10 methanol/water/formic acid. 2. Place the vessels in the ultrasonic bath for 30 minutes. 120 Februrary 11, 2013, Rev. B

121 Cleaning the ion block assembly 3. If you used formic acid in the cleaning solution, do as follows: a. Rinse the components by immersing them separately in glass vessels containing water and then placing the vessels in the ultrasonic bath for 20 minutes. b. Dry the components by immersing them in separate glass vessels containing methanol and then placing the vessels in the ultrasonic bath for 10 minutes. Caution: To avoid recontaminating the components, wear clean, chemical-resistant, powder-free gloves for the rest of this procedure. 4. Carefully remove the components from the vessels, and blow-dry them using inert, oil-free gas. 5. Inspect each component for persisting contamination. Requirement: If contamination is present, do as follows: a. Use the wash bottle containing 1:1 methanol/water to rinse the component over the large beaker. b. Blow-dry the component using inert, oil-free gas. Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the components according to local environmental regulations. 6. Inspect each component for persisting contamination. If contamination is present, dispose of the component, and obtain a new one before reassembly. Februrary 11, 2013, Rev. B 121

122 5 Maintenance Procedures Assembling the source ion block assembly Required materials Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool Isopropyl alcohol in small container 1:1 methanol/water Lint-free cloth To assemble the ion block assembly: Caution: To avoid recontaminating the ion block assembly, wear clean, chemical-resistant, powder-free gloves during this procedure. To avoid damaging the heater cartridge assembly wires, do not bend or twist them when fitting the assembly and ceramic heater mounting block to the ion block. 1. Carefully fit the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, to the ion block. 2. Use the combined 2.5-mm Allen wrench and cone extraction tool to tighten the captive PEEK terminal block securing screw. 3. Ensure that the grooves for the cover seal, cone gas O-ring, and isolation valve O-ring are free from dirt and debris. Tip: If contamination is present, use 1:1 methanol/water, applied to a lint-free cloth, to carefully clean the grooves. 4. Fit the cover seal (a new one if you disposed of the old seal) to the ion block, ensuring that it is correctly seated. 5. Fit the cone gas O-ring (a new one if you disposed of the old O-ring) to the ion block, ensuring that it is correctly seated. 6. Fit the O-ring to the isolation valve. Tip: If you use a new O-ring, first soak it for several minutes in isopropyl alcohol, so that it better fits the isolation valve. 122 Februrary 11, 2013, Rev. B

123 Cleaning the ion block assembly 7. Fit the isolation valve to the ion block assembly, so that it is in the closed position. 8. Fit the ion block cover plate to the ion block assembly, and then use the combined 2.5-mm Allen wrench and cone extraction tool to tighten the 2 captive securing screws for the ion block cover plate. 9. Hold the sampling cone assembly so that the cone gas nozzle handle is oriented horizontally and at the top, and then slide the sampling cone assembly into the ion block assembly. 10. Grasp the sampling cone assembly handle, and use it to rotate the sampling cone assembly through 90 degrees. Fitting the ion block assembly to the source assembly Required materials Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool To fit the ion block assembly to the source assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components components. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if one or both of the following conditions apply: An ESI probe is fitted (the probe tip is sharp). A corona pin is fitted (the pin tip is sharp). Caution: To avoid recontaminating the ion block assembly, wear clean chemical-resistant, powder-free gloves during this procedure. Februrary 11, 2013, Rev. B 123

124 5 Maintenance Procedures 1. Fit the ion block assembly to the PEEK ion block support. 2. Use the combined 2.5-mm Allen wrench and cone extraction tool to fit and then slowly tighten the 4 ion block assembly securing screws sequentially and in small increments. 3. Open the source isolation valve (see page 83). 4. Fit the source enclosure (see page 76). 5. Close the source enclosure. 6. Connect the power cords for the mass spectrometer and the roughing pump to the main power source. 124 Februrary 11, 2013, Rev. B

125 Cleaning the StepWave ion guide assembly Cleaning the StepWave ion guide assembly Clean the StepWave ion guide assembly if cleaning the ion block and isolation valve fails to increase signal sensitivity. Handling the StepWave ion guide assembly Caution: To avoid damaging the StepWave ion guide assembly, handle it and its components carefully throughout the cleaning procedure. In particular, to avoid damaging the wiring on assemblies fitted with an externally-wired printed circuit board (PCB), do not touch the wiring. To avoid damaging assemblies fitted with a flexible PCB, which is not externally wired, do not touch the PCB. Removing the ion block support from the source assembly Required materials Chemical-resistant, powder-free gloves 3-mm Allen wrench O-ring removal kit To remove the ion block support from the source assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. 1. Remove the source enclosure from the instrument (see page 73). 2. Remove the ion block assembly from the PEEK ion block support (see page 111). Februrary 11, 2013, Rev. B 125

126 5 Maintenance Procedures 3. Use the 3-mm Allen wrench to unscrew and remove the 4 screws securing the PEEK ion block support to the adaptor housing. Adaptor housing PEEK ion block support Securing screws StepWave assembly 4. Remove the PEEK ion block support from the adaptor housing. 5. Use the O-ring removal kit to carefully remove all the O-rings from the PEEK ion block support (see page 84). Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the O-rings according to local environmental regulations. 6. If any of the O-rings show signs of deterioration or damage, dispose of them in accordance with local environmental regulations. 126 Februrary 11, 2013, Rev. B

127 Cleaning the StepWave ion guide assembly Removing the StepWave assembly from the source assembly Required materials Chemical-resistant, powder-free gloves Seal breaker and locator tool StepWave assembly removal and insertion tool Recommendation: When not in use, store the seal breaker and locator tool on the end of the StepWave assembly removal and insertion tool. To remove the StepWave assembly from the source assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Caution: To avoid damaging the StepWave ion guide assembly when removing it from the source assembly, use only these tools: Seal breaker and locator tool StepWave assembly removal and insertion tool Do not use any other tool. 1. Position the seal breaker and locator tool so that it engages as follows: Its pin with the hole on the adaptor housing directly under the end of the StepWave ion guide assembly. Its lip behind the StepWave ion guide assembly s ion guide cap. Februrary 11, 2013, Rev. B 127

128 5 Maintenance Procedures Seal break and locator tool: Lip Pin Handle Seal breaker and locator tool positioned on the adaptor housing: Ion guide cap Adaptor housing Seal breaker and locator tool 128 Februrary 11, 2013, Rev. B

129 Cleaning the StepWave ion guide assembly 2. Push firmly on the seal breaker and locator tool s handle, to lever the StepWave assembly slightly out of the adaptor housing. Rationale: Moving the assembly in this manner releases it from a seal located inside the instrument. 3. With the StepWave removal and insertion tool s cutout uppermost, insert the tool s pins into the ion block support screw holes above and below the aperture in the pumping block assembly. Ion block support screw holes Cutout StepWave assembly removal and insertion tool Pins Brown PEEK ion guide cap Slot Caution: To avoid damage when removing the StepWave ion guide assembly from the adaptor housing, handle only the brown PEEK ion guide cap. 4. Inserting your thumbs through the slots in the StepWave removal and insertion tool, pull the StepWave ion guide assembly from the pumping block assembly and into the StepWave removal and insertion tool. Februrary 11, 2013, Rev. B 129

130 5 Maintenance Procedures Caution: To avoid damaging the StepWave ion guide assembly, handle it and its components carefully throughout the cleaning procedure. 5. Remove the StepWave ion guide assembly from the StepWave removal and insertion tool. 6. Using both hands, fit the source enclosure to the two supporting studs on the source adaptor housing. 7. Close the source enclosure. Rationale: Fitting and closing the source enclosure prevents debris entering the instrument while you are working on the StepWave ion guide assembly. 130 Februrary 11, 2013, Rev. B

131 Cleaning the StepWave ion guide assembly Disassembling the StepWave ion guide assembly Required materials Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool O-ring removal kit To disassemble the StepWave ion guide assembly: 1. Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the 2 screws securing the first ion guide assembly to the second ion guide assembly. First ion guide assembly Second ion guide assembly Securing screw Februrary 11, 2013, Rev. B 131

132 5 Maintenance Procedures 2. Separate the first and second ion guide assemblies. First ion guide assembly Second ion guide assembly 3. Remove the brown PEEK gasket from the second ion guide assembly. Brown PEEK gasket Second ion guide assembly 132 Februrary 11, 2013, Rev. B

133 Cleaning the StepWave ion guide assembly 4. Remove the O-ring from the differential pumping aperture on the second ion guide assembly (see page 84). Differential pumping aperture O-ring Second ion guide assembly Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the O-ring according to local environmental regulations. 5. If the O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations. Caution: To avoid misalignment of the StepWave ion guide, do not remove the differential pumping aperture or any further components from the assembly. Februrary 11, 2013, Rev. B 133

134 5 Maintenance Procedures Cleaning the StepWave ion guide assembly Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the first ion guide and second ion guide assemblies. Caution: To avoid damaging the first ion guide and second ion guide PCB assemblies, handle them carefully throughout this procedure. To avoid damaging the StepWave ion guide assembly, do not use acetone, chlorinated solvents, or acid as solvents when cleaning it. Use only the solvents specified in this procedure. Required materials Chemical-resistant, powder-free gloves. Two 250-mL measuring cylinders in which to completely immerse the first ion guide and second ion guide assemblies when cleaning. Two lengths of small-diameter PEEK or PTFE tubing appropriately sized for suspending the first ion guide and second ion guide assemblies in the glass vessels when cleaning. HPLC-grade deionized water. Waters MS Cleaning Solution. Holding container for used Waters MS Cleaning Solution. HPLC-grade isopropyl alcohol. Ultrasonic bath. Source of oil-free, inert gas (for example, nitrogen) for drying. 134 Februrary 11, 2013, Rev. B

135 Cleaning the StepWave ion guide assembly To clean the first ion guide and second ion guide PCB assemblies: 1. Bend a PEEK or PTFE tube into a hook shape. Caution: To avoid damage to the ion guide caused by vibration, ensure that the bottom of the ion guide does not touch the bottom of the glass vessel. 2. Use the hook to carefully suspend the first ion guide PCB assembly in the glass vessel so that the bottom of the assembly does not touch the bottom of the vessel. Hook First ion guide PCB assembly 3. Add Waters MS Cleaning Solution to the glass vessel until the first ion guide PCB assembly is immersed completely. Februrary 11, 2013, Rev. B 135

136 5 Maintenance Procedures 4. Repeat step 1 through step 3 for the second ion guide PCB assembly, placing the hook through one of the support rod holes. Hook Second ion guide PCB assembly 5. Place the vessels containing the first ion guide and second ion guide PCB assemblies in the ultrasonic bath for 20 minutes. 6. Carefully pour the cleaning solution from the vessel holding the first ion guide PCB assembly into the holding container, retaining the ion guide in the vessel. Tip: You can reuse the cleaning solution for one subsequent cleaning. 7. Fill the vessel with deionized water, to rinse the first ion guide PCB assembly, then discard the water. 8. Refill the vessel with deionized water, to rinse the first ion guide PCB assembly a second time, and then discard the water. 9. Repeat step 6 through step 8 for the second ion guide PCB assembly. 10. Fill both vessels with deionized water, ensuring that each ion guide PCB assembly is immersed completely. 11. Place the vessels containing the first ion guide and second ion guide PCB assemblies in the ultrasonic bath for 20 minutes. 136 Februrary 11, 2013, Rev. B

137 Cleaning the StepWave ion guide assembly 12. Carefully pour away and discard the deionized water from both vessels, retaining the ion guide PCB assemblies in each vessel. 13. Fill both vessels with isopropyl alcohol, ensuring that each ion guide PCB assembly is immersed completely. 14. Place the vessels containing the first ion guide and second ion guide PCB assemblies in the ultrasonic bath for 20 minutes. 15. Carefully remove each ion guide PCB assembly from its vessel, and blow-dry each assembly using inert, oil-free gas. 16. Discard the used isopropyl alcohol, using an appropriate waste container. Februrary 11, 2013, Rev. B 137

138 5 Maintenance Procedures Assembling the StepWave ion guide assembly Required materials Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool New O-ring PEEK gasket StepWave assembly securing screws (2) To assemble the StepWave ion guide assembly: Caution: To avoid damaging the StepWave ion guide assembly, handle it and its components carefully when performing this procedure. 1. Fit the new O-ring to the differential pumping aperture on the second ion guide assembly. Differential pumping aperture O-ring Second ion guide assembly 138 Februrary 11, 2013, Rev. B

139 Cleaning the StepWave ion guide assembly 2. Fit the brown PEEK gasket to the second ion guide assembly. Important: Ensure that the gasket is orientated correctly. Brown PEEK gasket Second ion guide assembly 3. Align the first ion guide assembly with the second ion guide assembly. 4. Use the combined, 2.5-mm, Allen wrench and cone extraction tool to fit and tighten the 2 screws securing the first ion guide assembly to the second ion guide assembly. Februrary 11, 2013, Rev. B 139

140 5 Maintenance Procedures Fitting the StepWave assembly to the source assembly Required materials Chemical-resistant, powder-free gloves Seal breaker and locator tool StepWave assembly removal and insertion tool To fit the StepWave assembly to the source assembly: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Caution: To avoid damage to the StepWave ion guide assembly when fitting it to the source assembly, use only the seal breaker and locator, and the assembly s removal and insertion tools. 1. Disconnect the probe adjuster and options cables from the instrument s connectors. 2. Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open the enclosure. 3. Using both hands, grasp the source enclosure, and lift it vertically off the two supporting studs on the source adaptor housing. 4. Slide the first ion guide assembly end of the StepWave assembly into the StepWave removal and insertion tool. Tip: The StepWave assembly can only be inserted in the correct orientation in the StepWave removal and insertion tool. 140 Februrary 11, 2013, Rev. B

141 Cleaning the StepWave ion guide assembly StepWave assembly Cutout Pins StepWave assembly removal and insertion tool 5. With the StepWave removal and insertion tool s cutout uppermost, insert the StepWave removal and insertion tool s pins into the ion block support screw holes above and below the aperture in the pumping block assembly. Caution: To avoid damage when fitting the StepWave ion guide assembly to the adaptor housing, handle only the brown PEEK ion guide cap. 6. Inserting your thumbs through the slots in the StepWave removal and insertion tool, firmly push the StepWave ion guide assembly into the pumping block assembly. Tip: You will detect some resistance to motion when the StepWave assembly encounters the seal inside the instrument; continue pushing until you detect further resistance. 7. Remove the StepWave removal and insertion tool. Februrary 11, 2013, Rev. B 141

142 5 Maintenance Procedures 8. Invert the seal breaker and locator tool, and locate it over the end of the StepWave assembly. Inverted seal breaker and locator tool StepWave assembly Adaptor housing 9. Push firmly on the seal breaker and locator tool until the tool s face contacts the adaptor housing. Rationale: This fully locates the StepWave assembly in the adaptor housing. 10. Remove the seal breaker and locator tool. 142 Februrary 11, 2013, Rev. B

143 Replacing the ESI probe tip and gasket Fitting the ion block support to the source Required materials Chemical-resistant, powder-free gloves 3-mm Allen wrench New seals and O-rings To fit the PEEK ion block support to the source: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. 1. Ensure that the grooves for the PEEK ion block support O-rings are free from dirt and debris. Tip: If contamination is present, use 1:1 methanol/water, applied to a lint-free cloth, to carefully clean the grooves. 2. Fit the O-rings (new ones if you disposed of the old) to the PEEK ion block support. Tip: To fit an O-ring in its groove, start fitting the O-ring at the notch in the groove, and then progressively work the ring into the groove, in either direction from the notch. 3. Fit the PEEK ion block support to the instrument s housing. 4. Use the 3-mm Allen wrench to fit and tighten the 4 PEEK ion block support securing screws. 5. Fit the ion block assembly to the PEEK ion block support (see page 123). 6. Fit the source enclosure to the instrument (see page 76). Replacing the ESI probe tip and gasket Replace the ESI probe tip if a blockage occurs in the internal metal sheathing through which the stainless steel capillary passes or if the probe tip is damaged. Februrary 11, 2013, Rev. B 143

144 5 Maintenance Procedures Removing the ESI probe tip and gasket Required materials Chemical-resistant, powder-free gloves 7-mm wrench 10-mm wrench To remove the ESI probe tip and gasket: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Warning: To avoid burn injuries, take great care while performing this procedure. Warning: To avoid puncture wounds, handle the probe with care. Its tip is sharp. 1. Remove the ESI probe from the source (see page 51). 2. Use the 10-mm wrench to remove the probe tip. 10-mm wrench Probe tip 144 Februrary 11, 2013, Rev. B

145 Replacing the ESI probe tip and gasket Tip: If the probe tip is difficult to remove, use the 7-mm wrench in conjunction with the 10-mm wrench. 7-mm wrench 10-mm wrench Probe tip 3. Remove the metal gasket from the probe tip. Metal gasket Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the probe tip and metal gasket according to local environmental regulations. 4. Dispose of the metal gasket in accordance with local environmental regulations. Februrary 11, 2013, Rev. B 145

146 5 Maintenance Procedures 5. If the probe tip is damaged, dispose of it in accordance with local environmental regulations. Fitting the ESI probe tip and gasket Required materials Chemical-resistant, powder-free gloves 10-mm wrench New metal gasket To fit the ESI probe tip and gasket: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Warning: To avoid puncture wounds, handle the probe with care. 1. Fit the new metal gasket into the probe tip. 2. Fit the probe tip over the capillary, and screw the tip onto the probe assembly. 3. Use the 10-mm wrench to tighten the probe tip. Important: To avoid gas leakage, fully tighten the probe tip. 4. Use the nebulizer adjuster knob to adjust the capillary so that it protrudes by approximately 0.5 mm from the end of the probe. 5. Fit the ESI probe to the source (see page 46). 146 Februrary 11, 2013, Rev. B

147 Replacing the ESI probe sample capillary Replacing the ESI probe sample capillary Replace the stainless steel sample capillary in the ESI probe if it becomes irreversibly blocked, or if it becomes contaminated or damaged. Removing the existing capillary Required materials Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool 7-mm wrench 8-mm wrench 10-mm wrench Needle-nose pliers To remove the existing capillary: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Warning: To avoid burn injuries, take great care while performing this procedure. Warning: To avoid puncture wounds, handle the probe with care.its tip is sharp. 1. Remove the probe from the source (see page 51). Februrary 11, 2013, Rev. B 147

148 5 Maintenance Procedures 2. Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the 3 probe end cover retaining screws. End cover retaining screws 3. Remove the end cover and gasket from the probe assembly. Nebulizer adjuster knob Gasket End-cover 148 Februrary 11, 2013, Rev. B

149 Replacing the ESI probe sample capillary 4. Unscrew and remove the nebulizer adjuster knob. 5. Use the 10-mm wrench to remove the probe tip. 10-mm wrench Probe tip Tip: If the probe tip is difficult to remove, use the 7-mm wrench in conjunction with the 10-mm wrench. 7-mm wrench 10-mm wrench Probe tip Februrary 11, 2013, Rev. B 149

150 5 Maintenance Procedures 6. Remove the metal gasket from the probe tip. Metal gasket 7. Remove the PEEK union/unf coupling assembly and capillary from the probe. PEEK union/unf coupling assembly Capillary 8. Unscrew and remove the knurled collar from the UNF coupling. PEEK union UNF coupling Locknut Conductive sleeve Knurled collar 9. Remove the knurled collar and conductive sleeve from the capillary. 10. Use the 7-mm wrench to loosen the locknut. Tip: Use the 8-mm wrench, to steady the UNF coupling when loosening the locknut. 150 Februrary 11, 2013, Rev. B

151 Replacing the ESI probe sample capillary 11. Unscrew the finger-tight PEEK union from the UNF coupling. Ferrule 12. Remove the ferrule and PTFE liner sleeve from the capillary. 13. Remove the capillary from the UNF coupling. 14. Dispose of the capillary, PTFE liner sleeve, and ferrule in accordance with local environmental regulations. Installing the new capillary Required materials PTFE liner sleeve Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the capillary, PTFE liner sleeve, and ferrule according to local environmental regulations. Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool 10-mm wrench Needle-nose pliers LC pump HPLC-grade (or better) 1:1 acetonitrile/water Capillary Ferrule Seal PTFE liner tubing Conductive sleeve Red PEEK tubing Februrary 11, 2013, Rev. B 151

152 5 Maintenance Procedures Metal gasket for the probe tip Sharp knife or PEEK tubing cutter Safety goggles To install the new capillary: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Warning: To avoid puncture wounds, handle the probe with care. 1. Use the sharp knife or PEEK tubing cutter to cut an approximately 60-cm (24-inches) length of red PEEK tubing. Requirement: Cut the tubing perpendicular to its horizontal axis. 2. Insert one end of the red, PEEK tubing in the probe inlet connector, and screw the connector, finger-tight, into the PEEK union. Rationale: Doing so ensures a minimum dead volume when fitting the capillary. Probe inlet connector PEEK tubing TP Fit the UNF coupling to the new capillary. 152 Februrary 11, 2013, Rev. B

153 Replacing the ESI probe sample capillary 4. Use the needle-nose pliers to slide a new liner sleeve and ferrule onto the capillary. 5. Insert the capillary in the PEEK union, and ensure that it is fully seated. 6. Screw the UNF coupling into the PEEK union, finger-tight only. 7. Gently tug on the capillary, testing to ensure that it stays in place. 8. Use the 7-mm wrench to tighten the locknut against the PEEK union until the union can no longer be twisted. 9. Slide a new conductive sleeve and the knurled collar over the capillary. 10. Tighten the knurled collar to the UNF coupling. Warning: To avoid high-pressure liquid jet spray, wear safety goggles when performing the leak test. 11. Perform a leak test by attaching the free end of the PEEK tubing to an LC pump and pumping 50:50 acetonitrile/water through it, at 1 ml/min. If leakage occurs, disassemble and remake the connection, and repeat the leak test. If the backpressure on the LC pump is high, replace the capillary, and repeat the leak test. 12. When no leakage occurs and the backpressure on the LC pump is normal, disconnect the PEEK tubing from the LC pump. 13. Remove the probe inlet connector and PEEK tubing from the PEEK union. 14. Carefully thread the capillary through the probe assembly. Februrary 11, 2013, Rev. B 153

154 5 Maintenance Procedures 15. Carefully push the PEEK union/unf coupling assembly and capillary into the probe assembly so that the locating pin on the UNF coupling is fully engaged in the locating slot at the head of the probe assembly. UNF coupling locating pin Probe assembly locating slot 16. Fit the nebulizer adjuster knob to the PEEK union/unf coupling assembly. 17. Finger-tighten the nebulizer adjuster knob onto the probe assembly. 18. Fit the gasket and the probe s end-cover to the probe assembly. 19. Use the combined 2.5-mm Allen wrench and cone extraction tool to fit and tighten the 3 probe end cover retaining screws. 20. Fit a new metal gasket to the probe tip. 21. Fit the probe tip over the capillary, and screw the tip onto the probe assembly. Caution: To avoid gas leakage, fully tighten the probe tip. 22. Use the 10-mm wrench to tighten the probe tip. 23. Use the nebulizer adjuster knob to adjust the capillary so that it protrudes by approximately 0.5 mm from the end of the probe tip. 24. Fit the ESI probe to the source (see page 46). 154 Februrary 11, 2013, Rev. B

155 Cleaning the IonSABRE II probe tip Cleaning the IonSABRE II probe tip Clean the IonSABRE II probe tip when you detect buffer buildup on the probe tip or when the signal intensity weakens. See the mass spectrometer s online Help for further details. To clean the IonSABRE II probe tip: 1. Stop all liquid flow through the probe, and then start the API gas flow. 2. Set Desolvation Gas to 650 L/h. 3. Set IonSABRE II probe Temp to 650 C. 4. Click Operate. 5. Wait 10 minutes. Rationale: The high IonSABRE II probe heater temperature removes any chemical contamination from the probe tip. 6. Click Standby. Replacing the IonSABRE II probe sample capillary Replace the stainless steel sample capillary in the IonSABRE II probe if it becomes blocked, and you cannot clear it, or if it becomes contaminated or damaged. Removing the existing capillary Required materials Chemical-resistant, powder-free gloves 7-mm wrench Combined 2.5-mm Allen wrench and cone extraction tool Februrary 11, 2013, Rev. B 155

156 5 Maintenance Procedures To remove the existing capillary: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Warning: To avoid burn injuries, take great care while performing this procedure when the probe and source are hot. 1. Remove the probe from the source (see page 55). 2. Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the 3 probe end cover retaining screws. 3. Use the combined 2.5-mm Allen wrench and cone extraction tool to remove the 3 probe end-cover retaining screws. End-cover retaining screws 156 Februrary 11, 2013, Rev. B

157 Replacing the IonSABRE II probe sample capillary 4. Remove the end cover and gasket. Nebulizer adjuster knob Gasket End-cover 5. Unscrew and remove the nebulizer adjuster knob. 6. Remove the PEEK union/unf coupling assembly and capillary from the probe. Tip: The PEEK union used with the IonSABRE II probe is notched on one of its flats, a feature that distinguishes it from the PEEK union used with the ESI probe (see Replacing the ESI probe sample capillary on page 147). PEEK union/unf coupling assembly Notch Capillary Locknut Februrary 11, 2013, Rev. B 157

158 5 Maintenance Procedures 7. Use the 7-mm wrench to loosen the locknut. 8. Unscrew the finger-tight PEEK union from the UNF coupling. Ferrule 9. Remove the ferrule from the capillary. 10. Remove the capillary from the UNF coupling. 11. Dispose of the capillary and ferrule in accordance with local environmental regulations. Installing the new capillary Required materials Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the capillary and ferrule according to local environmental regulations. Chemical-resistant, powder-free gloves Needle-nose pliers 7-mm wrench Combined 2.5-mm Allen wrench and cone extraction tool Red PEEK tubing LC pump HPLC-grade (or better) 1:1 acetonitrile/water Capillary Sharp knife or PEEK tubing cutter Safety goggles 158 Februrary 11, 2013, Rev. B

159 Replacing the IonSABRE II probe sample capillary To install the new capillary: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. 1. Use the sharp knife or PEEK tubing cutter to cut an approximately 60-cm (24-inches) length of red PEEK tubing. Requirement: Cut the tubing perpendicular to its horizontal axis. 2. Insert one end of the red, PEEK tubing in the probe inlet connector, and screw the connector, finger-tight, into the PEEK union. Rationale: Doing so ensures a minimum dead volume when fitting the capillary. Probe inlet connector PEEK tubing TP Fit the UNF coupling to the new capillary. 4. Fit the UNF coupling to the new capillary. Februrary 11, 2013, Rev. B 159

160 5 Maintenance Procedures Requirement: Use a UNF coupling with no grooves, which is appropriate to the IonSABRE II probe. 5. Use the needle-nose pliers to slide a new ferrule onto the capillary. 6. Insert the capillary in the PEEK union, and ensure that it is fully seated. 7. Screw the UNF coupling into the PEEK union, finger-tight only. 8. Gently tug on the capillary, testing to ensure that it stays in place. 9. Use the 7-mm wrench to tighten the locknut against the PEEK union. Warning: To avoid high-pressure liquid jet spray, wear safety goggles when performing the leak test. 10. Perform a leak test by attaching the free end of the PEEK tubing to an LC pump and pumping 50:50 acetonitrile/water through it at 1 ml/min. If leakage occurs, disassemble and remake the connection, and repeat the leak test. If the backpressure on the LC pump is high, replace the capillary, and repeat the leak test. 11. When no leakage occurs and the backpressure on the LC pump is normal, disconnect the PEEK tubing from the LC pump. 12. Remove the probe inlet connector and PEEK tubing from the PEEK union. 13. Remove the probe heater (see page 172, step 2). 14. Fit the PEEK union/unf coupling assembly to the nebulizer adjuster knob. 15. Carefully thread the capillary through the probe assembly. 160 Februrary 11, 2013, Rev. B

161 Replacing the IonSABRE II probe sample capillary 16. Carefully push the PEEK union/unf coupling assembly and capillary into the probe assembly so that the locating pin on the UNF coupling is fully engaged in the locating slot at the head of the probe assembly. UNF coupling locating pin Probe assembly locating slot 17. Fit the nebulizer adjuster knob to the PEEK union/unf coupling assembly. 18. Finger tighten the nebulizer adjuster knob onto the probe assembly. 19. Fit the probe gasket and end-cover to the probe assembly. 20. Use the combined 2.5-mm Allen wrench and cone extraction tool to fit and tighten the 3 end-cover securing screws. 21. Replace the combined 2.5-mm Allen wrench and cone extraction tool in its storage location on the source adaptor housing. Caution: To avoid damaging the probe heater s electrical connections, do not handle them. Hold the probe heater only by its body. Take great care not to damage the electrical connections, capillary sleeve, or capillary when fitting the heater over the capillary sleeve. 22. Fit the probe heater (see step 1 through step 3). 23. Fit the probe to the instrument (see page 52). 24. In the Instrument Console, click API to start the probe and desolvation gas flows. Februrary 11, 2013, Rev. B 161

162 5 Maintenance Procedures Replacing the LockSpray reference probe capillary Replace the LockSpray reference probe capillary if it becomes blocked, and you cannot clear it, or if it becomes contaminated or damaged. When replacing the reference probe capillary, you can also replace the microfilter. The microfilter is provided, with manufacturer s instructions for fitting it, in the spares kit for the reference probe assembly. Reference sprayer (behind baffle) Microfilter SealTight nut Reference probe capillary Removing the existing capillary Required materials Chemical-resistant, powder-free gloves 4-mm wrench Spares kit for the reference probe assembly Extender tool for the SealTight nut (included in the spares kit) 162 Februrary 11, 2013, Rev. B

163 Replacing the LockSpray reference probe capillary To remove the existing capillary: 1. Prepare the instrument for working on the source (see page 73). Warning: To avoid burn injuries, take great care while performing this procedure when the probe and source are hot. Warning: To avoid puncture wounds, if an ESI probe is fitted to the source, remove the probe before continuing with this procedure. 2. If an ESI probe is fitted to the source, remove it from the source (see page 51). 3. Pull the source enclosure release (located at the bottom, right-hand side) outwards, and swing open the enclosure. 4. Wait 10 minutes for the source ion block to cool. 5. Use the extender tool to disconnect the SealTight nut on the inside of the source enclosure. 6. Rotate the reference sprayer assembly clockwise through 90º, and remove it from the reference sprayer support assembly. Reference sprayer support assembly Reference sprayer assembly 7. Use the 4-mm wrench to unscrew and remove the reference probe tip from the reference sprayer assembly. Februrary 11, 2013, Rev. B 163

164 5 Maintenance Procedures 8. Unscrew the finger-tight nut on the rear of the reference sprayer assembly. 9. Remove the liner tubing and capillary from the reference sprayer assembly. 10. Remove the capillary from the liner tubing. 11. Dispose of the capillary in accordance with local environmental regulations. Installing the new capillary Required materials Chemical-resistant, powder-free gloves 4-mm wrench Spares kit for reference probe assembly Extender tool for the SealTight nut (included in the spares kit) To install the new capillary: 1. Fit the capillary into the liner tubing. Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the capillary according to local environmental regulations. 2. Fit the liner tubing and capillary to the reference sprayer assembly. 3. Fit the reference probe tip and, using the 4-mm wrench, screw it in until the end of the capillary protrudes 0.5 to 1.0 mm from the probe tip. Caution: To prevent pressure from ejecting the capillary from the sprayer, firmly tighten the finger-tight nut. 4. Tighten the finger-tight nut on the rear of the reference sprayer assembly. 5. Insert the reference sprayer assembly into its support assembly, with the sprayer tip pointing upwards. 164 Februrary 11, 2013, Rev. B

165 Replacing the NanoLockSpray reference probe capillary 6. Rotate the reference sprayer assembly 90º counterclockwise to lock it in place. Caution: To prevent pressure from forcing the tubing from the inside of the source enclosure, firmly tighten the SealTight nut. 7. Use the extender tool to tighten the SealTight nut on the inside of the source enclosure. 8. Close the source enclosure. Caution: To prevent pressure from ejecting the capillary from the sprayer, avoid flow rates exceeding 60 µl/min during normal operation. Replacing the NanoLockSpray reference probe capillary Replace the NanoLockSpray reference probe TaperTip or capillary if it is irreversibly blocked, or if it is contaminated or damaged. Removing the reference probe from the NanoLockSpray source Required materials Chemical-resistant, powder-free gloves 1.5-mm Allen wrench 5-mm Allen wrench Source of oil-free compressed gas for blow drying the union Wash bottle containing HPLC-grade (or better) 1:1 methanol/water Large beaker Februrary 11, 2013, Rev. B 165

166 5 Maintenance Procedures To remove the reference probe: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the plumbing, probe and source components. Warning: To avoid electric shock, ensure that the instrument is prepared for work performed on the source before commencing this procedure. 1. Prepare the instrument for working on the source (see page 73). 2. Disconnect the reference probe s cable from the instrument s high voltage connector. 3. Disconnect the fused silica capillary from the instrument s IntelliStart Fluidics system. 4. Use the combined 2.5-mm Allen wrench and cone extraction tool to unscrew the 3 reference probe retaining screws. Combined 2.5-mm Allen wrench and cone extraction tool Reference probe retaining screw Warning: To avoid puncture wounds, handle the reference probe carefully. Its TaperTip probe, which consists of 5 cm of exposed fused-silica, is fragile and extremely sharp. 166 Februrary 11, 2013, Rev. B

167 Replacing the NanoLockSpray reference probe capillary 5. Remove the reference probe from the probe adjuster assembly. 6. Unscrew the TaperTip PEEK coupler, and remove the TaperTip from the union. PEEK couplers Fused-silica capillary with PEEK protective sleeve TaperTip Fused-silica capillary Union Union retaining screw 7. Unscrew the capillary PEEK coupler, and remove the fused-silica capillary from the union. 8. Where appropriate, remove the protective PEEK sleeve from the fused-silica capillary for reuse. Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the fused-silica capillary according to local environmental regulations. 9. Dispose of the fused-silica capillary and TaperTip, observing local environmental regulations. 10. Using the 1.5-mm Allen wrench, unscrew the union retaining screw and remove the union. 11. Remove any remaining shards of glass or other contaminants by cleaning the union as follows: a. Use the wash bottle containing 1:1 methanol/water to rinse the union over the large beaker. Februrary 11, 2013, Rev. B 167

168 5 Maintenance Procedures b. Clear and blow dry the union with compressed gas. Installing the new TaperTip and capillary Required materials Chemical-resistant, powder-free gloves Combined 2.5-mm Allen wrench and cone extraction tool Fused-silica cutting tool Pin-plug capillary locator TaperTip A 375-mm length of 25-µL, fused-silica capillary, sleeved for protection by inch ID PEEK tubing To install the new TaperTip and capillary: 1. On the reference probe assembly, screw the pin-plug capillary locator into the end of the union. Union Pin-plug capillary locator Reference-probe body 2. Thread the capillary through the body of the reference probe. 3. Slide a PEEK coupler over the end of the fused-silica capillary. 168 Februrary 11, 2013, Rev. B

169 Replacing the NanoLockSpray reference probe capillary 4. Locate the PEEK coupler in the union, and carefully slide the fused-silica capillary into the union until it butts against the pin-plug capillary locator. Rationale: The pin-plug capillary locator correctly locates the capillary in the union and ensures a minimum dead volume when fitting the TaperTip. 5. Tighten the PEEK coupler to hold the capillary in place. 6. Unscrew and remove the pin-plug capillary locator. Warning: To avoid puncture wounds, handle the reference probe carefully. Its TaperTip probe, which consists of 5 cm of exposed fused-silica, is fragile and extremely sharp. Caution: Ensure that you are inserting the square-cut end of the TaperTip into the union and not the tapered tip. 7. Align the PEEK coupler in the end of the union and slide the TaperTip into the union until it butts against the fused-silica capillary. Caution: Overtightening the coupler on the TaperTip crushes the TaperTip and causes blockages. 8. Finger-tighten the coupler to hold the TaperTip securely without crushing. Caution: The fragile TaperTip at the end of the reference probe is easily broken during insertion into the source. 9. Mount the reference probe on the NanoLockSpray source. 10. Use the combined 2.5-mm Allen wrench and cone extraction tool to fit and tighten the 3 reference probe retaining screws. 11. Using an F-130 Finger-tight Nut, connect the fused-silica capillary to the grounded union in the instrument s IntelliStart Fluidics system. 12. Connect the reference probe s cable to the instrument s high voltage connector. Februrary 11, 2013, Rev. B 169

170 5 Maintenance Procedures Cleaning or replacing the corona pin Required materials Chemical-resistant, powder-free gloves Needle-nose pliers HPLC-grade (or better) methanol Lint-free tissue Lapping film Corona pin To clean or replace the corona pin: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. Warning: To avoid burn injuries, take great care while performing this procedure when the probe and source are hot. Warning: To avoid electric shock, ensure that the instrument is in Standby mode before commencing this procedure. Warning: To avoid puncture wounds, handle the corona pin with care. 1. Remove the corona pin from the source (see page 80). 2. Replace the corona pin if it is deformed or otherwise damaged. Otherwise clean the tip of the pin with the lapping film, and then wipe it clean with a methanol-saturated tissue. Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the corona pin according to local environmental regulations. 170 Februrary 11, 2013, Rev. B

171 Replacing the IonSABRE II probe heater 3. If you are replacing the corona pin, dispose of the old pin in accordance with local environmental regulations. 4. Install the corona pin in the source (see page 77). Replacing the IonSABRE II probe heater Replace the IonSABRE II probe heater it fails to heat the probe. Removing the IonSABRE II probe heater Required materials Chemical-resistant, powder-free gloves To remove the IonSABRE II probe heater: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. 1. Remove the probe from the source (see page 55). Probe heater Februrary 11, 2013, Rev. B 171

172 5 Maintenance Procedures Caution: To avoid damaging the probe heater s electrical connections, do not twist the heater when removing it from the probe assembly. 2. Gripping the probe heater as shown, carefully pull it off the probe assembly. Probe heater Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the probe heater according to local environmental regulations. 3. Dispose of the probe heater in accordance with local environmental regulations. 172 Februrary 11, 2013, Rev. B

173 Replacing the IonSABRE II probe heater Fitting the new IonSABRE II probe heater Required materials Chemical-resistant, powder-free gloves IonSABRE II probe heater To fit the new IonSABRE II probe heater: Caution: Take great care not to damage the probe heater s electrical connections, capillary sleeve, or capillary when fitting the heater over the capillary sleeve. 1. Use the probe adjuster knob to adjust the capillary so that it protrudes approximately 0.5 mm from the end of the probe. Probe heater connections 0.5 mm Capillary TP02670 Capillary sleeve 2. Carefully slide the probe heater over the capillary sleeve on the probe assembly. Caution: To avoid damaging the probe heater s electrical connections, do not twist the heater when fitting it to the probe assembly. 3. Fit the probe heater to the probe assembly, ensuring that the heater is fully seated on the probe assembly. 4. Fit the probe to the instrument (see page 52). 5. In the Instrument Console, click API to start the desolvation gas. Februrary 11, 2013, Rev. B 173

174 5 Maintenance Procedures Replacing the ion block source heater Replace the ion block source heater if it fails to heat the ion block when the instrument is pumped-down (evacuated). Required materials Chemical-resistant, powder-free gloves Needle-nose pliers Combined 2.5-mm Allen wrench and cone extraction tool New ion block source heater assembly To replace the ion block source heater: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the ion block assembly. 1. Remove the ion block assembly from the instrument (see page 111). 2. Ensure that the isolation valve is closed. Isolation valve handle in closed position 174 Februrary 11, 2013, Rev. B

175 Replacing the ion block source heater 3. Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate. Ion block cover plate securing screw Ion block cover plate 4. Remove the ion block cover plate. 5. Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw. Heater cartridge assembly wires PEEK terminal block securing screw Februrary 11, 2013, Rev. B 175

176 5 Maintenance Procedures Caution: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when removing the assembly and ceramic heater mounting block from the ion block. 6. Carefully remove the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, from the ion block. Tip: You can invert the ion block assembly to facilitate this process. Heater cartridge wire securing screws Ceramic heater mounting block PEEK terminal block 7. Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the 2 screws securing the heater wires to the PEEK terminal block. 8. Disconnect the heater cartridge wires from the PEEK terminal block. 176 Februrary 11, 2013, Rev. B

177 Replacing the ion block source heater 9. Use the needle-nose pliers to gently grasp the heat-shrink tubing on the heater cartridge assembly, and slide the assembly out of the ceramic heater mounting block. Heat-shrink tubing Heater cartridge assembly Ceramic heater mounting block 10. Dispose of the heater cartridge assembly. Caution: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when fitting the assembly to the ceramic heater mounting block. 11. Use the needle-nose pliers to gently grasp the heat-shrink tubing on the new heater cartridge assembly, and slide the assembly into the ceramic heater mounting block. 12. Use the needle-nose pliers to position the heater cartridge s wiring ring tags fully under their securing screws. 13. Use the combined 2.5-mm Allen wrench and cone extraction tool to tighten the 2, wire-securing screws. 14. Fit the PEEK terminal block and ceramic heater mounting block, complete with heater cartridge assembly, to the ion block. 15. Use the combined 2.5-mm Allen wrench and cone extraction tool to tighten the captive, PEEK terminal block securing screw. 16. Fit the ion block cover plate to the ion block assembly, and then use the combined 2.5-mm Allen wrench and cone extraction tool to tighten the 2 captive screws securing ion block cover plate. 17. Fit the ion block assembly to the instrument (see page 123). Februrary 11, 2013, Rev. B 177

178 5 Maintenance Procedures Replacing the LockSpray source s assembly seals Note: You do not need to replace the NanoLockSpray source s assembly seals; this section relates to the LockSpray source only. To avoid excessive leakage of solvent vapor into the laboratory atmosphere, the following seals must be renewed at intervals of no greater than 1 year: Probe adjuster assembly probe seal Probe adjuster assembly nebulization gas seal Source enclosure seal Source enclosure nebulizer gas seal Source enclosure desolvation gas seal Removing the probe adjuster assembly probe and source enclosure seals Required materials Chemical-resistant, powder-free gloves O-ring removal kit To remove the probe adjuster assembly probe and source enclosure seals: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. 1. Remove the source enclosure from the instrument (see page 73). 2. Use the O-ring removal kit to carefully remove the following seals from the probe adjuster assembly: Probe seal Nebulizer gas seal 178 Februrary 11, 2013, Rev. B

179 Replacing the LockSpray source s assembly seals See Removing O-rings and seals on page 84. Probe adjuster nebulizer gas seal Probe adjuster assembly probe seal 3. Use the O-ring removal kit to carefully remove the following seals from the source enclosure: Source enclosure seal Nebulizer gas seal Desolvation gas seal Februrary 11, 2013, Rev. B 179

180 5 Maintenance Procedures Nebulizer gas seal Desolvation gas seal Source enclosure seal 4. Dispose of all the seals in accordance with local environmental regulations. Fitting the new source enclosure and probe adjuster assembly probe seals Required materials Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of the seals according to local environmental regulations. Chemical-resistant, powder-free gloves Wash bottle containing HPLC-grade (or better) 1:1 methanol/water New seals Lint-free cloth 180 Februrary 11, 2013, Rev. B

181 Replacing the LockSpray source s assembly seals To fit the new source enclosure and probe adjuster assembly probe seals: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the probe and source components. 1. Ensure that all the grooves for seals are free from dirt and debris. Tip: If contamination is present, use 1:1 methanol/water, applied to a lint-free cloth, to carefully clean the grooves. 2. Fit the new source enclosure seal to the source enclosure. Important: Ensure that the tails of the source enclosure seals are correctly located in the groove when fitting them to the source enclosure. Start by feeding the seal into the groove at the bottom right-hand corner, then work around the seal in the counterclockwise direction. 3. Fit the following new seals to the source enclosure: Nebulizer gas seal Desolvation gas seal Requirement: Fit the seals, which incorporate a special cross-section, into grooves, as shown. Seal Groove 4. Fit the following new seals to the probe adjuster assembly: Probe seal Nebulizer gas seal 5. Fit the source enclosure to the instrument (see page 76). Februrary 11, 2013, Rev. B 181

182 5 Maintenance Procedures Replacing the mass spectrometer s air filters Replacing the air filter inside the front door Required materials Needle-nose pliers New filter To replace the air filter inside the front door: 1. Open the access door to the fluidics pump (see the figure on page 22). 2. Unscrew the captive thumbscrew on the filter cover. Thumbscrew Filter cover 182 Februrary 11, 2013, Rev. B

183 Replacing the mass spectrometer s air filters 3. Remove the filter cover from the instrument. Filter Filter cover 4. Lift the filter, vertically, from its slot in the instrument. Tip: If necessary, use the needle-nose pliers to grasp the filter. 5. Dispose of the filter. 6. Fit the new filter into the instrument. 7. Fit the filter cover to the instrument. 8. Tighten the thumbscrew on the filter cover. 9. Close the access door to the fluidics pump. Februrary 11, 2013, Rev. B 183

184 5 Maintenance Procedures Replacing the air filters on the sides of the instrument Air filters are located on both sides of the instrument. These filters are retained by louvered panels, which you must remove to access the filters. Filter panel on the right-hand side of the instrument: Note: Two similar, smaller panels are located on the left-hand side of the instrument. Air filter panel 184 Februrary 11, 2013, Rev. B

185 Replacing the mass spectrometer s air filters Required materials M4 POZIDRIV screwdriver New filters To replace the air filters on the sides of the instrument: For each filter, follow this sequence of actions: 1. Use the M4 POZIDRIV screwdriver to remove the screws securing the louvered panel to the instrument enclosure. 2. Remove the louvered panel from the instrument enclosure. 3. Remove the filter from the instrument. 4. Dispose of the filter. 5. Fit the new filter into the instrument. 6. Fit the louvered panel to the instrument enclosure. 7. Use the M4 POZIDRIV screwdriver to fit and tighten the screws securing the louvered panel to the instrument enclosure. Februrary 11, 2013, Rev. B 185

186 5 Maintenance Procedures Replacing the IntelliStart Fluidics tubing In the event of a blockage in the tubing connections between the IntelliStart Fluidics system components, you must replace the tubing. The following procedures explain how to replace the tubing for the LockSpray and sample delivery systems. They do not, however, address probe connections, which vary according to your application. For probe connection instructions, see the relevant procedure: For the ESI probe, see page 46. For the IonSABRE II probe, see page 52. Fit tubing according to one of these two plumbing configurations: LockSpray standard configuration (see page 187). NanoLockSpray configuration (see page 198). 186 Februrary 11, 2013, Rev. B

187 Replacing IntelliStart Fluidics tubing (Standard configuration) Replacing IntelliStart Fluidics tubing (Standard configuration) IntelliStart Fluidics system physical layout: The flow sensor and grounded union are optional fittings if you fit the LockSpray source. They are, however, standard fittings if you fit the NanoLockSpray source. Tip: Tubing connections are omitted for clarity. Access doors Lock-spray selector valve Tubing guides Flow sensor Grounded union A B AA B Sample selector valve C C Diverter valve Lock-spray pump A B C Sample pump Waters A B C Sample reservoir bottles (A, B, and C) Februrary 11, 2013, Rev. B 187

188 5 Maintenance Procedures Removing the IntelliStart Fluidics tubing This procedure explains how to remove the IntelliStart Fluidics tubing, and disconnect the probe tubing at the diverter valve or grounded union. Required materials Chemical-resistant, powder-free gloves To remove the tubing: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with tubing and fittings. 1. Open the access doors to the IntelliStart Fluidics system. 2. Unscrew and remove the PEEK thumbscrews and tubes from the lock-spray selector, sample selector, and diverter valves. 3. Unscrew and remove the PEEK thumbscrews and tubes from between the flow sensor and the grounded union, if these are used. 4. Close the access doors to the IntelliStart Fluidics system. Warning: To avoid contaminating uncontaminated surfaces with biologically hazardous, toxic, or corrosive materials, dispose of IntelliStart Fluidics tubing and fittings according to local environmental regulations. 5. Dispose of the tubes and fittings in accordance with local environmental regulations. 188 Februrary 11, 2013, Rev. B

189 Replacing IntelliStart Fluidics tubing (Standard configuration) Plumbing the IntelliStart Fluidics lock-spray system This section explains how to plumb the lock-spray system. Requirement: Finger tighten all PEEK fittings. Tubing schematic for the lock-spray system: From external reference bottle (optional) Flow sensor (optional) From wash bottle To reference probe Tubing guides A B Grounded union (optional) C 4 5 Lock-spray selector valve Waste port From pump Tubing dimensions (lock-spray system): Port number Connection ID (inch) OD (inch) Color Length (mm) 1 Reservoir bottle A /16 Orange Reservoir bottle B /16 Orange Reservoir bottle C /16 Orange Reference probe (or Flow sensor if fitted) /32 Red Waste reservoir /16 Natural Wash reservoir /16 Orange Lock-spray selector pump /16 Blue Flow sensor to grounded union (if used) /32 Red 60 - Grounded union to reference probe Probe and flow rate dependent. Februrary 11, 2013, Rev. B 189

190 5 Maintenance Procedures Required materials Chemical-resistant, powder-free gloves. The Xevo G2-S QTof Fluidics Tubing and Fitting Kit. Tip: This kit contains components for both the sample and lock-spray system plumbing. For the LockSpray reference probe connection, 375 mm of inch ID red PEEK A long finger-tight fitting To plumb the lock-spray system: Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with tubing and fittings. Requirement: Finger tighten all PEEK fittings. 1. Open the access doors to the IntelliStart Fluidics system. 2. Using a PEEK nut, Super Flangeless ferrule, and stainless steel (SS) ring, connect the blue, 1/16-inch, 300-mm, PEEK tubing from the 190 Februrary 11, 2013, Rev. B

191 Replacing IntelliStart Fluidics tubing (Standard configuration) lock-spray pump to port 7 on the lock-spray selector valve, where you use a long finger-tight fitting. A A B B Lock-spray selector valve C C Lock-spray pump A B C Waters A B C PEEK nut, Super Flangeless ferrule, and stainless steel (SS) ring: PEEK nut Super Flangeless ferrule Stainless steel ring Long finger-tight fitting: Februrary 11, 2013, Rev. B 191

192 5 Maintenance Procedures 3. Using a long finger-tight fitting, connect orange, 1/16-inch, 680-mm, PEEK tubing from port 1 of the lock-spray selector valve, through tubing guide A and into reservoir bottle A. A A B B C C A B C Waters A B C Reservoir bottle A Requirement: As the tubing emerges from the tubing guide, thread a long finger-tight fitting over it. Push the tubing through the left-hand hole, to the bottom of the reservoir bottle, and tighten the fitting. Tips: Follow these suggestions if you encounter difficulty threading the tubing through the guides: Thread the tubing upward from the bottom hole. Using needle-nose pliers, make a 20 bend, 10 mm from the end of the tubing. Then rotate the tubing, as necessary, as you thread it through the guide. 4. Using a long finger-tight fitting, connect orange, 1/16-inch, 680-mm, PEEK tubing from port 2 of the lock-spray selector valve to the reference reservoir bottle or orange, 1/16-inch, 1000-mm, PEEK tubing to the external reference bottle. To use reservoir bottle B as the reference solution, thread the tubing through tubing guide B, and secure it with a long finger-tight fitting. 192 Februrary 11, 2013, Rev. B

193 Replacing IntelliStart Fluidics tubing (Standard configuration) To use an external reference bottle, push the tubing to the bottom of the reference solution, and secure the tubing at the bottle neck to prevent it floating to the surface during use. 5. Using a long finger-tight fitting, connect orange, 1/16-inch, 680-mm, PEEK tubing from port 3 of the lock-spray selector valve selector valve through tubing guide C and into reservoir bottle C. Requirement: As the tubing emerges from the tubing guide, thread the long finger-tight fitting over it, push the tubing through the left-hand hole to the bottom of the reservoir bottle, and finger tighten the fitting. 6. Using a long finger-tight fitting, connect orange, 1/16-inch, 1000-mm, PEEK tubing from port 6 of the lock-spray selector valve to the wash bottle. Requirement: Push the tubing to the bottom of the wash solution, and secure the tubing to prevent it floating to the surface during use. 7. Using a long finger-tight fitting, connect 1/16-inch, 1000-mm, natural-color PEEK tubing to port 5 of the lock-spray selector valve, and thread the tubing into the waste port. A A B B Waste port C C A B C Waters A B C Tip: The liquid waste system collects waste without requiring a fitted connection. Waste drains through the connector at the base of the instrument and into a waste bottle (see page 239). Februrary 11, 2013, Rev. B 193

194 5 Maintenance Procedures 8. If the system lacks an optional flow sensor and grounded union, skip to step Februrary 11, 2013, Rev. B

195 Replacing IntelliStart Fluidics tubing (Standard configuration) 9. Using a long finger-tight PEEK nut and 1/32-inch PEEK ferrule, connect the red, 1/32-inch, 200-mm, PEEK tubing from port 4 of the lock-spray selector valve to the left-hand side of the flow sensor, where you use the 1/32-inch, 6-40 Valco compression fitting assembly. Flow sensor A A B B C C A B C Waters A B C Long finger-tight PEEK nut and 1/32-inch, PEEK ferrule: 1/32-inch, 6-40 Valco compression fitting assembly: Februrary 11, 2013, Rev. B 195

196 5 Maintenance Procedures 10. Using a 1/32-inch, Valco component fitting assembly, connect the red, 1/32-inch, 60-mm, PEEK tubing between the flow sensor and the grounded union, where you use a use a short finger-tight nut and 1/32-inch ferrule. Important: The narrower, 1/32-inch tubing can slip out of the port. After tightening the fitting, gently tug the tubing, to ensure it is secure in the port. Grounded union A A B B C C A B C Waters A B C Short finger-tight nut and 1/32-inch ferrule: 196 Februrary 11, 2013, Rev. B

197 Replacing IntelliStart Fluidics tubing (Standard configuration) Warning: To avoid electric shock, do not use stainless steel tubing to connect the grounded union to the reference probe. 11. Connect the grounded union to the LockSpray source s reference probe. For the LockSpray source, use 1/16-inch PEEK tubing connected as follows: At the grounded union end, use a long finger-tight fitting. Long finger-tight fitting: At the source reference-probe connection, use the PEEK, finger-tight nut and ferrule. PEEK, finger-tight nut and ferrule: For the NanoLockSpray source, see page Close the access doors to the IntelliStart Fluidics system. Important: Ensure that the tubing does not become trapped when you close the access door to the IntelliStart Fluidics system. Februrary 11, 2013, Rev. B 197

198 5 Maintenance Procedures Replacing IntelliStart Fluidics tubing (NanoLockSpray) This section explains how to plumb the sample delivery system for NanoLockSpray standard flow applications. For low flow, nanoacquity UPLC applications, see page 204. Plumbing the IntelliStart Fluidics sample delivery system IntelliStart Fluidics sample delivery tubing schematic: From wash bottle Sample selector valve Tubing guides A B To probe C Waste port 4 Diverter valve From pump All tubing is PEEK, except for that comprising the connection between the pump and sample selector valve, which is stainless steel. Tubing dimensions (sample delivery system): Valve/port Connection ID (inch) OD (inch) From UPLC Color Length (mm) Sample/1 Waste reservoir /16 Natural 1000 Sample/2 Wash reservoir /16 Orange 1000 Sample/3 Diverter valve /16 Red 200 Sample/4 Reservoir Bottle A /16 Orange 680 Sample/5 Reservoir Bottle B /16 Orange 680 Sample/6 Reservoir Bottle C /16 Orange Februrary 11, 2013, Rev. B

199 Replacing IntelliStart Fluidics tubing (NanoLockSpray) Tubing dimensions (sample delivery system): Valve/port Connection Sample/7 Analyte pump /16 n/a 500 Divert/4 Waste /16 Natural 1000 Required materials Chemical-resistant, powder-free gloves The Xevo G2-S QTof Fluidics Tubing and Fitting Kit Tip: This kit contains components for both the sample and lock-spray system s plumbing. To plumb the analyte system: ID (inch) OD (inch) Color Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with tubing and fittings. 1. Open the access doors to the IntelliStart Fluidics system. Length (mm) 2. Using the stainless steel tubing, connect the sample pump to port 7 of the sample selector valve. Februrary 11, 2013, Rev. B 199

200 5 Maintenance Procedures Requirement: The steel tubing requires high-pressure seals at each end. You must tighten the fittings appropriately. A B C A B C Sample selector valve A B C Waters Lock-spray pump A B C a. Slide the PEEK nut, Super Flangeless ferrule, and stainless steel ring over the pump end of the steel tubing. PEEK nut Super Flangeless ferrule Stainless steel tubing Stainless steel ring b. Insert the tubing in the pump, and tighten the fittings. c. At the sample selector valve, screw the natural-color PEEK female-to-male adaptor into port 7. PEEK female-to-male adaptor Flangeless nut Flangeless ferrule 200 Februrary 11, 2013, Rev. B

201 Replacing IntelliStart Fluidics tubing (NanoLockSpray) d. Slide the transparent, 1/16-inch flangeless nut and blue, 1/16-inch flangeless ferrule over the tubing. Rationale: These components provide fail-safe pressure relief in the event of a blockage. e. Insert the tubing in the female-to-male adaptor in port 7, and tighten the fittings. 3. Using a long finger-tight fitting, connect 1/16-inch, 1000-mm, natural-colored PEEK tubing to port 1 of the sample selector valve, and thread the tubing into the waste port. A A B B C C Waste port A B C Waters A B C Long finger-tight fitting: Tip: The liquid waste system collects waste without requiring a fitted connection. Waste drains through the connector at the base of the instrument and into the waste bottle (see page 239). 4. Using a long finger-tight fitting, connect orange, 1/16-inch, 1000-mm, PEEK tubing from port 2 of the sample selector valve to the wash bottle. Februrary 11, 2013, Rev. B 201

202 5 Maintenance Procedures Requirement: Push the tubing to the bottom of the wash solution, and secure so that it cannot float to the surface during use. 5. Using a long finger-tight fitting, connect orange, 1/16-inch, 680-mm, PEEK tubing from port 4 of the sample selector valve, through tubing guide A and into reservoir bottle A. Tubing guide A A A B B C C A B C Waters A B C Reservoir Bottle A Requirement: As the tubing emerges from the tubing guide, thread the long finger-tight fitting over it, push the tubing through the right-hand hole, to the bottom of the reservoir bottle, and tighten the fitting. Tips: Follow these suggestions if you encounter difficulty threading the tubing through the guides: Thread the tubes upward from the bottom hole. Using needle-nose pliers, make a 20 bend, 10 mm from the end of the tubing. Then rotate the tubing, as necessary, as you thread it through the guide. 6. Repeat this procedure for port 5 to reservoir bottle B and port 6 to reservoir bottle C. 202 Februrary 11, 2013, Rev. B

203 Replacing IntelliStart Fluidics tubing (NanoLockSpray) 7. Using two long finger-tight fittings, connect the red, 1/16-inch, 200-mm, PEEK tubing from port 3 of the sample selector valve to port 3 of the divert valve. A A B C B C Divert valve A B C Waters A B C 8. Using a long finger-tight fitting, connect 1/16-inch, 1000-mm, natural-colored PEEK tubing to port 4 of the divert valve, and thread it securely into the waste port. Tip: This is the same waste port as that used in step 3. A A B B C C Waste port A B C Waters A B C Februrary 11, 2013, Rev. B 203

204 5 Maintenance Procedures 9. Connect port 2 of the diverter valve to the source probe, observing the relevant procedure: For the ESI probe, see page 46. For the IonSABRE II probe, see page Close the access doors to the IntelliStart Fluidics system. Important: Ensure that the tubing does not become trapped when you close the access doors to the IntelliStart Fluidics system. Plumbing the IntelliStart Fluidics for low flow sample delivery This section explains how to plumb the sample delivery system for low flow, nanoacquity applications. IntelliStart Fluidics sample delivery tubing schematic: From wash bottle Sample selector valve From grounded union (LockSpray) Tubing guides A B To reference sprayer C Waste port 4 Diverter valve Pump All tubing is of PEEK, except for that comprising the connection between the pump and sample selector valve, which is stainless steel. 204 Februrary 11, 2013, Rev. B

205 Plumbing the IntelliStart Fluidics for low flow sample delivery Tubing dimensions (sample delivery system): Valve/Port Connection Required materials Chemical-resistant, powder-free gloves The Xevo G2-S QTof Fluidics Tubing and Fitting Kit Tip: This kit contains components for both the sample and NanoSpray system s plumbing. Needle-nose pliers To plumb the analyte system: ID (inch) Requirement: Finger tighten all PEEK fittings. OD (inch) Color 1. Open the access door to the IntelliStart Fluidics system. Length (mm) Sample/1 Waste reservoir /16 Natural 1000 Sample/2 Wash reservoir /16 Orange 1000 Sample/3 Diverter valve /16 Red 200 Sample/4 Reservoir Bottle A /16 Orange 680 Sample/5 Reservoir Bottle B /16 Orange 680 Sample/6 Reservoir Bottle C /16 Orange 680 Sample/7 Analyte pump /16 n/a 500 Divert/4 Waste /16 Natural 1000 Warning: To avoid personal contamination with biologically hazardous, toxic, or corrosive materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with IntelliStart Fluidics tubing and fittings. 2. Using the stainless steel tubing, connect the sample pump to port 7 of the sample selector valve. Februrary 11, 2013, Rev. B 205

206 5 Maintenance Procedures Requirement: The steel tubing requires high-pressure seals at each end. You must tighten the fittings appropriately. A B C A B C Sample selector valve A B C Waters Lock-spray pump A B C a. Slide the PEEK nut, stainless steel ring, and Super Flangeless ferrule over the pump end of the steel tubing. PEEK nut Super Flangeless ferrule Stainless steel tubing Stainless steel ring b. Insert the tubing in the pump, and tighten the fittings. c. At the sample selector valve, screw the natural-color PEEK female-to-male adaptor into port 7. PEEK female-to-male adaptor Flangeless nut Flangeless ferrule d. Slide the transparent, 1/16 inch flangeless nut and blue 1/16 inch flangeless ferrule over the tubing. 206 Februrary 11, 2013, Rev. B

207 Plumbing the IntelliStart Fluidics for low flow sample delivery Rationale: These components provide fail-safe pressure relief in the event of a blockage. e. Insert the tubing in the female-to-male adaptor in port 7, and tighten the fittings. 3. Using a long finger-tight fitting, connect 1/16 inch, 1000 mm, natural-colored PEEK tubing to port 1 of the sample selector valve, and thread the tubing into the waste port. A A B B C C Waste port A B C Waters A B C Long finger-tight fitting: Tip: The liquid waste system collects waste without requiring a fitted connection. Waste drains through the connector at the base of the instrument and into the waste bottle (see page 239). 4. Using a long finger-tight fitting, connect orange 1/16 inch, 1000 mm, PEEK tubing from port 2 of the sample selector valve to the wash bottle. Requirement: Push the tubing to the bottom of the wash solution, and secure it to prevent the tubing floating to the surface during use. Februrary 11, 2013, Rev. B 207

208 5 Maintenance Procedures 5. Using a long finger-tight fitting, connect orange 1/16 inch, 680 mm, PEEK tubing from port 4 of the sample selector valve, through tubing guide A and into reservoir bottle A. Tubing guide A A A B B C C A B C Waters A B C Reservoir Bottle A Requirement: As the tubing emerges from the tubing guide, thread the long finger-tight fitting over it, push the tubing through the right-hand hole, to the bottom of the reservoir bottle and tighten the fitting. Tips: Follow these suggestions if you encounter difficulty threading the tubing through the guides: Thread the tubes upward from the bottom hole. Using needle-nose pliers, make a 20 bend, 10 mm from the end of the tubing. Then rotate the tubing, as necessary, as you thread it through the guide. 6. Repeat this procedure for port 5 to reservoir bottle B and port 6 to reservoir bottle C. 208 Februrary 11, 2013, Rev. B

209 Plumbing the IntelliStart Fluidics for low flow sample delivery 7. Using two long finger-tight fittings, connect the red 1/16 inch, 200 mm, PEEK tubing from port 3 of the sample selector valve to port 3 of the diverter valve. A A B C B C Diverter valve A B C Waters A B C 8. Using a long finger-tight fitting, connect 1/16 inch, 1000 mm, natural-colored PEEK tubing to port 4 of the diverter valve, and thread it securely into the waste port. Tip: This is the same waste port as that used in step 3. A A B B C C Waste port A B C Waters A B C Februrary 11, 2013, Rev. B 209

210 5 Maintenance Procedures 9. Connect port 2 of the diverter valve to the source probe, observing the relevant procedure for the ESI probe (see page 46). 10. Close the access doors to the IntelliStart Fluidics system. Important: Ensure that the tubing does not become trapped when you close the access doors to the IntelliStart Fluidics system. 210 Februrary 11, 2013, Rev. B

211 A Safety Advisories Waters instruments display hazard symbols designed to alert you to the hidden dangers of operating and maintaining the instruments. Their corresponding user guides also include the hazard symbols, with accompanying text statements describing the hazards and telling you how to avoid them. This appendix presents all the safety symbols and statements that apply to the entire line of Waters products. Contents: Topic Page Warning symbols Caution symbol Warnings that apply to all Waters instruments and devices Electrical and handling symbols Februrary 11, Rev. B 211

212 A Safety Advisories Warning symbols Warning symbols alert you to the risk of death, injury, or seriously adverse physiological reactions associated with an instrument s use or misuse. Heed all warnings when you install, repair, and operate Waters instruments. Waters assumes no liability for the failure of those who install, repair, or operate its instruments to comply with any safety precaution. Task-specific hazard warnings The following warning symbols alert you to risks that can arise when you operate or maintain an instrument or instrument component. Such risks include burn injuries, electric shocks, ultraviolet radiation exposures, and others. When the following symbols appear in a manual s narratives or procedures, their accompanying text identifies the specific risk and explains how to avoid it. Warning: (General risk of danger. When this symbol appears on an instrument, consult the instrument s user documentation for important safety-related information before you use the instrument.) Warning: (Risk of burn injury from contacting hot surfaces.) Warning: (Risk of electric shock.) Warning: (Risk of fire.) Warning: (Risk of sharp-point puncture injury.) Warning: (Risk of hand crush injury.) Warning: (Risk of exposure to ultraviolet radiation.) Warning: (Risk of contacting corrosive substances.) Warning: (Risk of exposure to a toxic substance.) 212 Februrary 11, Rev. B

213 Warning symbols Warning: (Risk of personal exposure to laser radiation.) Warning: (Risk of exposure to biological agents that can pose a serious health threat.) Warning: (Risk of tipping.) Warning: (Risk of explosion.) Specific warnings The following warnings can appear in the user manuals of particular instruments and on labels affixed to them or their component parts. Burst warning This warning applies to Waters instruments fitted with nonmetallic tubing. Warning: Pressurized nonmetallic, or polymer, tubing can burst. Observe these precautions when working around such tubing: Wear eye protection. Extinguish all nearby flames. Do not use tubing that is, or has been, stressed or kinked. Do not expose nonmetallic tubing to incompatible compounds like tetrahydrofuran (THF) and nitric or sulfuric acids. Be aware that some compounds, like methylene chloride and dimethyl sulfoxide, can cause nonmetallic tubing to swell, which significantly reduces the pressure at which the tubing can rupture. Februrary 11, Rev. B 213

214 A Safety Advisories Mass spectrometer flammable solvents warning This warning applies to instruments operated with flammable solvents. Warning: Where significant quantities of flammable solvents are involved, a continuous flow of nitrogen into the ion source is required to prevent possible ignition in that enclosed space. Ensure that the nitrogen supply pressure never falls below 690 kpa (6.9 bar, 100 psi) during an analysis in which flammable solvents are used. Also ensure a gas-fail connection is connected to the LC system so that the LC solvent flow stops if the nitrogen supply fails. Mass spectrometer shock hazard This warning applies to all Waters mass spectrometers. Warning: To avoid electric shock, do not remove the mass spectrometer s protective panels. The components they cover are not user-serviceable. This warning applies to certain instruments when they are in Operate mode. Warning: High voltages can be present at certain external surfaces of the mass spectrometer when the instrument is in Operate mode. To avoid non lethal electric shock, make sure the instrument is in Standby mode before touching areas marked with this high voltage warning symbol. 214 Februrary 11, Rev. B

215 Caution symbol Biohazard warning This warning applies to Waters instruments that can be used to process material that can contain biohazards: substances that contain biological agents capable of producing harmful effects in humans. Warning: Waters instruments and software can be used to analyze or process potentially infectious human-sourced products, inactivated microorganisms, and other biological materials. To avoid infection with these agents, assume that all biological fluids are infectious, observe Good Laboratory Practice, and consult your organization s biohazard safety representative regarding their proper use and handling. Specific precautions appear in the latest edition of the US National Institutes of Health (NIH) publication, Biosafety in Microbiological and Biomedical Laboratories (BMBL). Chemical hazard warning This warning applies to Waters instruments that can process corrosive, toxic, flammable, or other types of hazardous material. Warning: Waters instruments can be used to analyze or process potentially hazardous substances. To avoid injury with any of these materials, familiarize yourself with the materials and their hazards, observe Good Laboratory Practice (GLP), and consult your organization s safety representative regarding proper use and handling. Guidelines are provided in the latest edition of the National Research Council's publication, Prudent Practices in the Laboratory: Handling and Disposal of Chemicals. Caution symbol Caution advisories appear where an instrument or device can be subject to use or misuse capable of damaging it or compromising a sample s integrity. The exclamation point symbol and its associated statement alert you to such risk. Caution: To avoid damaging the instrument s case, do not clean it with abrasives or solvents.. Februrary 11, Rev. B 215

216 A Safety Advisories Prohibition symbol Prohibition symbols signify an action you must not perform under any circumstances. The following symbol and its associated statement indicates a prohibited action that applies to the Xevo G2-S QTof. Warning: Do not place containers on top of the instrument or on its front covers. Instead, use the bottle tray. 216 Februrary 11, Rev. B

217 Warnings that apply to all Waters instruments and devices Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Attention: Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment. Important: Toute modification sur cette unité n ayant pas été expressément approuvée par l autorité responsable de la conformité à la réglementation peut annuler le droit de l utilisateur à exploiter l équipement. Achtung: Jedwede Änderungen oder Modifikationen an dem Gerät ohne die ausdrückliche Genehmigung der für die ordnungsgemäße Funktionstüchtigkeit verantwortlichen Personen kann zum Entzug der Bedienungsbefugnis des Systems führen. Avvertenza: qualsiasi modifica o alterazione apportata a questa unità e non espressamente autorizzata dai responsabili per la conformità fa decadere il diritto all'utilizzo dell'apparecchiatura da parte dell'utente. Atencion: cualquier cambio o modificación efectuado en esta unidad que no haya sido expresamente aprobado por la parte responsable del cumplimiento puede anular la autorización del usuario para utilizar el equipo. 注意 : 未經有關法規認證部門允許對本設備進行的改變或修改, 可能會使使用者喪失操作該設備的權利 注意 : 未经有关法规认证部门明确允许对本设备进行的改变或改装, 可能会使使用者丧失操作该设备的合法性 주의 : 규정준수를책임지는당사자의명백한승인없이이장치를개조또는변경할경우, 이장치를운용할수있는사용자권한의효력을상실할수있습니다. 注意 : 規制機関から明確な承認を受けずに本装置の変更や改造を行うと 本装置のユーザーとしての承認が無効になる可能性があります Februrary 11, Rev. B 217

218 A Safety Advisories Warning: Use caution when working with any polymer tubing under pressure: Always wear eye protection when near pressurized polymer tubing. Extinguish all nearby flames. Do not use tubing that has been severely stressed or kinked. Do not use nonmetallic tubing with tetrahydrofuran (THF) or concentrated nitric or sulfuric acids. Be aware that methylene chloride and dimethyl sulfoxide cause nonmetallic tubing to swell, which greatly reduces the rupture pressure of the tubing. Attention: Manipulez les tubes en polymère sous pression avec precaution: Portez systématiquement des lunettes de protection lorsque vous vous trouvez à proximité de tubes en polymère pressurisés. Eteignez toute flamme se trouvant à proximité de l instrument. Evitez d'utiliser des tubes sévèrement déformés ou endommagés. Evitez d'utiliser des tubes non métalliques avec du tétrahydrofurane (THF) ou de l'acide sulfurique ou nitrique concentré. Sachez que le chlorure de méthylène et le diméthylesulfoxyde entraînent le gonflement des tuyaux non métalliques, ce qui réduit considérablement leur pression de rupture. Vorsicht: Bei der Arbeit mit Polymerschläuchen unter Druck ist besondere Vorsicht angebracht: In der Nähe von unter Druck stehenden Polymerschläuchen stets Schutzbrille tragen. Alle offenen Flammen in der Nähe löschen. Keine Schläuche verwenden, die stark geknickt oder überbeansprucht sind. Nichtmetallische Schläuche nicht für Tetrahydrofuran (THF) oder konzentrierte Salpeter- oder Schwefelsäure verwenden. Durch Methylenchlorid und Dimethylsulfoxid können nichtmetallische Schläuche quellen; dadurch wird der Berstdruck des Schlauches erheblich reduziert. 218 Februrary 11, Rev. B

219 Warnings that apply to all Waters instruments and devices Attenzione: fare attenzione quando si utilizzano tubi in materiale polimerico sotto pressione: Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. Spegnere tutte le fiamme vive nell'ambiente circostante. Non utilizzare tubi eccessivamente logorati o piegati. Non utilizzare tubi non metallici con tetraidrofurano (THF) o acido solforico o nitrico concentrati. Tenere presente che il cloruro di metilene e il dimetilsolfossido provocano rigonfiamenti nei tubi non metallici, riducendo notevolmente la pressione di rottura dei tubi stessi. Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión: El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos de polímero sometidos a presión. Si hubiera alguna llama las proximidades. No se debe trabajar con tubos que se hayan doblado o sometido a altas presiones. Es necesario utilizar tubos de metal cuando se trabaje con tetrahidrofurano (THF) o ácidos nítrico o sulfúrico concentrados. Hay que tener en cuenta que el cloruro de metileno y el sulfóxido de dimetilo dilatan los tubos no metálicos, lo que reduce la presión de ruptura de los tubos. 警告 : 當在有壓力的情況下使用聚合物管線時, 小心注意以下幾點 當接近有壓力的聚合物管線時一定要戴防護眼鏡 熄滅附近所有的火焰 不要使用已經被壓癟或嚴重彎曲管線 不要在非金屬管線中使用四氫呋喃或濃硝酸或濃硫酸 要了解使用二氯甲烷及二甲基亞楓會導致非金屬管線膨脹, 大大降低管線的耐壓能力 Februrary 11, Rev. B 219

220 A Safety Advisories 警告 : 当有压力的情况下使用管线时, 小心注意以下几点 : 当接近有压力的聚合物管线时一定要戴防护眼镜 熄灭附近所有的火焰 不要使用已经被压瘪或严重弯曲的管线 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀, 大大降低管线的耐压能力 경고 : 가압폴리머튜브로작업할경우에는주의하십시오. 가압폴리머튜브근처에서는항상보호안경을착용하십시오. 근처의화기를모두끄십시오. 심하게변형되거나꼬인튜브는사용하지마십시오. 비금속 (Nonmetallic) 튜브를테트라히드로푸란 (Tetrahydrofuran: THF) 또는농축질산또는황산과함께사용하지마십시오. 염화메틸렌 (Methylene chloride) 및디메틸술폭시드 (Dimethyl sulfoxide) 는비금속튜브를부풀려튜브의파열압력을크게감소시킬수있으므로유의하십시오. 警告 : 圧力のかかったポリマーチューブを扱うときは 注意してください 加圧されたポリマーチューブの付近では 必ず保護メガネを着用してください 近くにある火を消してください 著しく変形した または折れ曲がったチューブは使用しないでください 非金属チューブには テトラヒドロフラン (THF) や高濃度の硝酸または硫酸などを流さないでください 塩化メチレンやジメチルスルホキシドは 非金属チューブの膨張を引き起こす場合があり その場合 チューブは極めて低い圧力で破裂します 220 Februrary 11, Rev. B

221 Warnings that apply to all Waters instruments and devices Warning: The user shall be made aware that if the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Attention: L utilisateur doit être informé que si le matériel est utilisé d une façon non spécifiée par le fabricant, la protection assurée par le matériel risque d être défectueuses. Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwenddung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren. Attenzione: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura. Advertencia: el usuario deberá saber que si el equipo se utiliza de forma distinta a la especificada por el fabricante, las medidas de protección del equipo podrían ser insuficientes. 警告 : 使用者必須非常清楚如果設備不是按照製造廠商指定的方式使用, 那麼該設備所提供的保護將被消弱 警告 : 使用者必须非常清楚如果设备不是按照制造厂商指定的方式使用, 那么该设备所提供的保护将被削弱 경고 : 제조업체가명시하지않은방식으로장비를사용할경우장비가제공하는보호수단이제대로작동하지않을수있다는점을사용자에게반드시인식시켜야합니다. 警告 : ユーザーは 製造元により指定されていない方法で機器を使用すると 機器が提供している保証が無効になる可能性があることに注意して下さい Februrary 11, Rev. B 221

222 A Safety Advisories Electrical and handling symbols Electrical symbols These can appear in instrument user manuals and on the instrument s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse 222 Februrary 11, Rev. B