Efficiency of inoculant powder. Examination about efficiency of RhizoNat Extra natural soybean inoculant powder :

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1 Efficiency of inoculant powder Examination about efficiency of RhizoNat Extra natural soybean inoculant powder :

2

3 The effect of the inoculant powder on soybean plant

4 Wheat yield was close to 25% more after soybean forecrop Application Composition: Ground Bradyrhizobium japonicum nodule with soil (99.9 m/m%), Rhodamine B 500 dye (0.1 m/m%). Active ingredient content: Total number of germs is minimum 1, pcs/g. Application: The sowing seed is evenly moistened in a proportion of 0.4

5 litre water/100 kg sowing seed by continuous stirring and then the inoculant is poured onto the wet seed by continuous stirring (in a proportion of 100 gram inoculant/100 kg sowing seed). Continue stirring until the seeds become pink. Sowing seed not treated with powder inoculant.

6 Sowing seed treated with powder inoculant. After treatment the sowing seed must be dried. The germination ability of bacteria Bradyrhizobim japonicum on dry seed is best before 70 days. Seed inoculation Soybean Seed Inoculation* Soybean seed inoculation, its bacterial treatment, is necessary primarily in areas where soybean has not yet been grown, therefore the Rhizobium japonicum bacterium could not proliferate in the soil. On farmlands where soybean is regularly grown, the soil is rich in the bacterium, so

7 repeated inoculation is unnecessary. (At the same time, in the USA, the No.1 soybean producing country in the world, it is considered to be efficient to use inoculated seeds every third year even in areas where soybean has been grown for a long time.) Based on experiences from practice, it was noted already in the mid-19th century that plants of the bean family are nitrogen fixers, and this characteristic of theirs is connected to their root tubers. In 1888, a connection between plants of the bean family and the Rhizobium bacterium was also observed (Hellriegel Willfart). Over the next few years, the first usable inoculant, the Nitragin, was placed in the market. Inoculation of the soil and, later on, of the seeds has been practised ever since the late 19th century. Rhizobium japonicum is able to fix atmospheric nitrogen especially in symbiosis with the soybean. Nitrogen fixation is a series of complex chemical processes, through which the nitrogen molecules of the air are fixed or, rather, reduced to ammonium, in the root tubers and, in this form, they become a usable source of nitrogen even for the host plant. The amount of atmospheric N fixed in this way is significant, the largest part of it nourishes the soybean, while part of it is utilized by the soil and the catch crop. (It is a general experience from practice that cereals which grow after the soybean in the autumn make good use of the leftover nitrogen.) These days, with evergrowing fertilizer prices, it is worth considering to utilize the nitrogen offered to the farmers by nature itself. For this, the seed just needs to be treated with bacteria, with inoculant powder, since Rhizobium japonicum is not endemic to the Hungarian soil. Recently, the distribution of inoculated soybean seeds treated by coating has become more and more widespread especially in the

8 corporate practice of foreign companies in Hungary, and it is now followed by an increasing number of Hungarian distributors of seeds. This way, the producer is relieved from the delicate and tedious task of inoculating the soybean seeds. The seeds should be treated in accordance with the user manual; if it prescribes treatment on moistened seeds, never sprinkle the powder dry into the grain box or distributor of the seed drill. In case you are planning to inoculate the seeds yourself, it is useful to note a few facts about the Rhizobium japonicum bacteria. Fixation of atmospheric N is subject to several conditions: suitable temperature is between 15 and 25 degrees Celsius, high nitrogenation of the soil reduces their action, extreme drought and, on the other hand, exceedingly moist, airless soil with residual water are equally undesirable, fixation of atmospheric N fails in acidic soil, at the same time, Ca, Mo, Fe and Co have a favourable effect on their action. Rhizobium bacteria are quite sensitive to environmental factors, so inoculation has to be performed with great care: the inoculant has to be applied, in accordance with the instruction for its use, evenly and gently. (If no better equipment is available, a low-rpm concrete mixer will also do.) Always treat only that much amount of seeds which is going to be planted in the course of one day; do not store the inoculated seeds. Protect the inoculant and the inoculated seeds from direct light. Whenever possible, perform the inoculation in a shady or closed area protected from direct light.

9 In ideal conditions, we can observe the efficiency of the inoculation, and that of the fixation of atmospheric N, as early as in the fourth or fifth week of vegetation, when we can find tubers the size of a pinhead. Later, the larger yellowish, pinkish cutting surface of the tubers indicates the process of active fixation of atmospheric nitrogen. * Source: Sándor Balikó (2015) Szójatermesztés korszerűen, S- Press 5 Kft., p. About Soybean inoculant powder RhizNat Extra natural soybean inoculant powder: The RhizoNat Extra természetes szója oltópor ( RhizoNat Extra natural soybean inoculant powder) is a soybean inoculant that has a high nitrogen fixing ability, contains Bradyrhizobia, is of natural origin, increases yield, and can be used for organic production. The so-called Bradyrhizobium bacteria and the stocks and biotypes of the Bradyrhizobium japonicum bacteria species have a special role in soy cultivation. If these exist in the soil they fix atmospheric nitrogen in the nodules growing on the roots of the soy and transfer it to the plant. As a result the host-plant (soy) produces bigger yield and enriches the soil with nitrogen.

10 Soy nodule on the roots of the plant Originally there is no soy Rhizobium in European soils. Therefore it is necessary to treat the seeds to be sown with preparations and inoculants containing special Bradyrhizobium japonicum stocks. Soybean inoculant powder As a result of the procedure applied to prepare the RhizoNat Extra természetes szója oltópor the Bradyrhizobia obtained propagate well in all soils that can be found in plough-land cultivation and have a good nitrogen fixing ability. In our process the propagation of Bradyrhizobium stocks is not performed on artificial culture medium (in vitro) but in the nodules growing on the roots of soy cultivated in soil, or by the collection of the nodules (in vivo). All of soybean

11 varieties are without GMO. The inoculant has a very good effect as it does not contain only one Rhizobium stock but a population of Bradyrhizobia stocks that populate best under natural conditions and have the best nitrogen fixing ability. The inoculant lasts longer and has a longer lifetime than any other inoculant known so far as it contains only 5% water. Keeping Bradyrhizobia, resistant to dehydration, in a dry and cool place they retain their maximal infection and nitrogen fixing ability for more than two years. When using the inoculant powder the soy plant fixes kg nitrogen from the atmosphere per hectare; 50% of it is used by the plant itself and the rest remains in the soil for the subsequent plant.

Prepare a good seed bed and after sowing, roll to compact the soil and ensure good soil contact with the seeds.

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