IOWA STATE UNIVERSITY 2016 IOWA EVALUATION OF INSECTICIDES AND PLANT-INCORPORATED PROTECTANTS

Transcription

1 IOWA STATE UNIVERSITY OF SCIENCE AND TECHNOLOGY 0 IOWA EVALUATION OF INSECTICIDES AND PLANT-INCORPORATED PROTECTANTS CORN PESTS RESEARCH PROJECT DEPARTMENT OF ENTOMOLOGY AMES, IOWA 00-0 SUE BLODGETT, CHAIR INSECT INVESTIGATED Corn Rootworm Diabrotica virgifera virgifera and Diabrotica barberi Report Authors March 9, 0 Aaron Gassmann File number: 0- Patrick Weber

2 0 IOWA EVALUATION OF INSECTICIDES AND PLANT INCORPORATED PROTECTANTS TABLE OF CONTENTS Page. Research Personnel.... Introduction and Objective.... Testing Procedures and Evaluations.... Results and Discussion.... Studies Conducted Corn Rootworm Evaluation Tables Ames (Johnson Farm) Comparison among Bt Corn Traits Root Injury/Percent Consistency... 9 AMVAC Aztec-HC Efficacy and Yield Study Root Injury/Percent Consistency... 9 Stand Count... 0 Yield... 0 FMC Evaluations Root Injury/Percent Consistency... Stand Count... Yield... FMC Experimental Capture RIVE D on Bt Corn Root Injury/Percent Consistency... Stand Count... Yield... FMC Experimental Capture RIVE D on non-rw Bt Corn Root Injury/Percent Consistency... Stand Count... Yield...

3 Table of Contents (Continued) Page Ames (Johnson Farm)-Continued Neudorff Study on non-rw Bt corn Root Injury/Percent Consistency... Stand Count... Yield... Valent Ampex Study Root Injury/Percent Consistency... Stand Count... Yield... 8 Boone (Bruner Farm) Comparison among Bt Corn Traits Root Injury/Percent Consistency... 8 Dow AgroSciences Study Root Injury/Percent Consistency... 9 Stand Count... 9 Yield... 9 FMC Evaluations Root Injury/Percent Consistency... 0 Stand Count... 0 Yield... FMC Experimental Capture RIVE D on Bt Corn Root Injury/Percent Consistency... Stand Count... Yield... Crawfordsville Valent Ampex Study Root Injury/Percent Consistency... Stand Count... Lodging... Yield...

4 Table of Contents (Continued) Page Nashua Comparison among Bt Corn Traits Root Injury/Percent Consistency... AMVAC Aztec HC Efficacy and Yield Study Root Injury/Percent Consistency... Stand Count... Lodging... Yield... Marrone Bio Innovations Study Root Injury/Percent Consistency... Stand Count... 8 Lodging... 8 Yield... 9 Valent Ampex Study Root Injury/Percent Consistency... 9 Stand Count... 0 Lodging... 0 Yield.... Appendix I - Agronomic Information Appendix II Weather Data Appendix III - Materials Tested Appendix IV - Research Pictures... 8

5 0 IOWA EVALUATION OF INSECTICIDES AND PLANT INCORPORATED PROTECTANTS CORN PESTS RESEARCH PROJECT DEPARTMENT OF ENTOMOLOGY AMES, IOWA 00-0 Project Leader: Agricultural Specialist: Postdoctoral Research Associate: Research Associate Graduate Research Assistants: Technical Assistants: University Research Farm Superintendents: Aaron Gassmann Patrick Weber Ram Shrestha Ben Brenizer Eric Clifton Kenneth Masloski Coy St. Clair Eric Yu Pierre Alameda Elisabeth Anderson Nao Demand Colton Schaben Devon Shields Kent Berns Mike Fiscus Dallas Maxwell Kenneth Pecinovsky Myron Rees Terry Tuttle

6 INTRODUCTION In Iowa, the species of corn rootworm (CRW) include western corn rootworm Diabrotica virgifera virfigera LeConte and northern corn rootworm D. barberi Smith and Lawrence, and these two species are the most damaging pests of corn, Zea mays, in the Unites States Corn Belt. Eggs are laid in the soil during the summer and hatch the following spring. Larval feeding on corn roots in June may diminish yield by reducing plant growth and drought tolerance, and by imposing harvest losses due to plant lodging. Adult emergence from the soil is underway by early July and continues through late summer. Additional crop losses may be caused by adult beetles feeding on the corn silk and on soft doughy kernels. In Iowa, crop rotation, where it fits cropping practices, remains the preferred method of management. However, it is also economically feasible to protect corn roots with soil applied insecticides and plant-incorporated protectants, which are transgenic corn hybrids that produce insecticidal toxins derived from Bacillus thuringiensis (Bt). In additional to corn rootworm, several aboveground lepidopteran pests feed on corn in Iowa. These include the European corn borer Ostrinia nubilalis, black cutworm Agrotis ipsilon, fall armyworm Spodoptera frugiperda, and corn earworm Helicoverpa zea. Over the last decade, Bt corn varieties have been made commercially available to protect corn from these pests. OBJECTIVE The goal of this research program is to serve the agricultural community of Iowa by monitoring and evaluating the performance of registered and precommercial insecticides and transgenic corn hybrids. To achieve this goal, we maintain a viable, proactive, progressive and scientifically sound product evaluation program. TESTING PROCEDURES AND EVALUATIONS Field Sites: Research plots were established at four Iowa State University research farms in 0: ) Johnson Farm (Ames), ) Bruner Farm (Boone), ) Northeast Research and Demonstration Farm (Nashua) and ) Southeast Research and Demonstration Farm (Crawfordsville). At all locations, two sections of the study site are rotated annually as either research plots or lateplanted trap crop. The seed planted for the trap crop is a mixed maturity blend with a greater proportion of latematuring varieties. This trap crop constitutes a favorable environment for adult female CRW late in the season when other fields are maturing, and can result in a high abudance of CRW larvae the following season. Table lists the studies conducted at each location. METHODS Field plot design: With the exception of the Southeast Research and Demonstartion Farm (Crawfordsville), the experimental design was a randomized complete block with either four or six replications. At the Crawfordsville location, the experimental design had four replications of the two treatments, and these were planted in an alternating pattern. Plot lengths and the number of replications per study are provided in Table.

7 Planting: Seeds were pre-bagged in the laboratory and then planted with a fourrow John Deere Max Emerge TM 00 Integral Rigid Frame Planter that had 0 inch row spacing. Seeds were planted at a depth of inches with a spacing of 0. inches between seeds (,00 seeds per acre). The standard planter fiberglass seed hoppers with attached finger-pickup mechanism were replaced with modified units. On these modified units, the metal plate that covered the fingers had been replaced with a /8 inch clear Plexiglas plate. Inserted through the Plexiglas was a small stainless steel cylinder. The cylinder was positioned to deliver seed to the pickup fingers. At the beginning of each replication, pre-bagged seeds were dumped into the steel cylinder. At the beginning of each replication, a hydraulic motor (attached to the planter s drive shaft) was activated to deliver seed immediately into the ground. At the end of each replication, this same hydraulic motor was activated to expel any unplanted seed, and the finger pickup mechanism was visually inspected through the clear Plexiglas plate to ensure that no seed was present. Planting of three FMC studies (two replicates of FMC Experimental Capture RIVED on non-rw Bt corn and one replicate of FMC Experimental Capture RIVED on Bt Corn), was accomplished with a two-row John Deere planter that had 0 inch row spacing and bulk seed hoppers. Plant-incorporated protectants: Plantincorporated protectants were evaluated in corn hybrids producing insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt), and these are described in Appendix III. Seed treatments: All the DeKalb hybrids were treated with Poncho 00. All the Mycogen hybrids were treated with Cruiser Extreme 0. All the Syngenta hybrids were treated with Cruiser 0 or 0. All the Pioneer hybrids were treated with either Cruiser 0 + Maxim Quattro + Poncho0/Votivo + PPCT/, or Cruiser 0 + Maxim Quattro + PPCT0. These seed treatments target secondary pests, such as wireworm and seed corn maggot but may have some, limited efficacy against CRW larave at levels higher than 0 mg active ingredient per kernel. Granular soil-applied insecticides: The granular insecticide Force.0G was applied with modified Noble metering units mounted on the planter. The Noble units were calibrated in the laboratory to accurately deliver material at a tractor speed of mph. Force.0G insecticide was applied with in-furrow placement. For the evaluation of Antixx Plus, this product was hand applied for both infurrow and T-Band (eight inches wide) placement in the respective treatments. This Antixx Plus product was weighted out into deli style containers for application in the field. Liquid soil-applied insecticides: Ampex.SC, Capture LFR.SC, Ethos LFR.0SC, Ethos XB.SC, Expedient.0SC, Force 0CS, Tepera Plus 0.8SC, Venerate XC and MBI- 0BMSE were applied in-furrow at planting with a compressed-air system built directly into the planter by Almaco manufacturing (Nevada, IA). Ampex

8 .SC, Capture LFR.SC, Ethos LFR.0SC, Ethos XB.SC, Expedient.0SC, Force 0CS and Tepera Plus 0.8SC were applied as ounces per 000 row feet. Venerate XC and MBI- 0BMSE were applied as gallons per acre. All liquid insecticide was applied with a Teejet XR800EVS spray nozzles at psi to deliver GPA of finished spray at a tractor speed of mph. Ampex, Ethos LFR, Ethos XB, Expedient and Tepera Plus was applied using a starter fertilizer (Nucleus HP 8- -) as the carrier. Capture LFR was applied using both water and starter fertilizer (Nucleus HP 8--) as the carrier. Force 0CS, MBI-0BMSE and Venerate XC used water as the carrier. Liquid products applied in the three FMC studies included Capture LFR.SC, Capture RIVE D.SC, Ethos LFR SC, Ethos XB.SC, Force 0CS, VGR.0WP. These studies were planted with a two row John Deere 00 planter that had 0 inch row spacing. All insecticides were applied as fluid ounces per 000 row feet except for VGR.0WP, which was applied as grams per acre. Capture RIVE D and Force used water as the carrier and all other products used fertilizer (Nucleus Hyperlink HP 8--) as a carrier. All these products were applied in-furrow at a tractor speed of mph. SmartBox TM application of soilapplied insecticides: Aztec-SB.G, and Aztec-HC 9.G insecticide treatments were applied with modified SmartBox TM metering units mounted on the planter. The commercial SmartBox TM units were removed from their large-base containers and sandwiched between a flat metal plate on the bottom and a custom-made, threaded plastic cap on the top. The bottom plate had been fabricated so that it could slide in and out of the same planter mounting brackets used for the Noble units. An inverted L Nalgene bottle attached to the top provided a secure and sealed container for insecticide for the SmartBox TM units. Clear plastic tubes directed the granular insecticides to both the in-furrow and T- band placement. A controller mounted in the tractor cab was used to operate all the SmartBox TM metering units. These units were managed using the AMVAC SmartBox System Manager. All treatments were applied at mph using the fixed speed mode on the SmartBox TM controllers. These SmartBox TM metering units were calibrated before going to the field to deliver ounces of product per 000 row feet. General methodology: During planting, each row was constantly monitored to ensure that insecticides were applied correctly. Final incorporation was accomplished with drag chains mounted behind the closing wheels. Evaluations Stand counts: The number of plants in. row-ft was recorded. This was done early and late in the growing season and then averaged to provide one value for stand counts for each treatment in each block. Root injury: After the majority of corn rootworms had finished feeding, roots were dug to assess feeding injury. This took place from July to August,

9 0. Prior to leaving the field, excess soil was removed from roots and all roots were labeled with study name and plot number using a permanent marker. Roots were cleaned at the ISU Johnson Farm s root washing station. These roots were first soaked in water for between and hours and then washed with a hose to remove any remaining soil. Roots were evaluated for rootworm feeding injury following the Iowa State 0 to Node-Injury Scale: Node-Injury Scale (0-): No feeding injury (lowest rating that can be given)..0 - One node (circle of roots), or the equivalent of an entire node, pruned to within. inches of the stalk or soil line Two nodes pruned Three or more nodes pruned. (Highest rating that can be given) Injury in between complete nodes pruned is noted as the percentage of the node missing (e.g.,.0 = one and a half nodes pruned and 0. = one quarter of one node pruned). The linear node-injury scale allows injury to be expressed intuitively and has proved useful in evaluating minor injury. For an interactive guide to the node-injury scale, see the website for the Department of Entomology at Iowa State University. Lodging counts: A plant was considered lodged if it was leaning at least 0 degrees from vertical. These data were taken at harvest time along with final stand counts. Yields: Studies that were taken to yield were machine harvested with a modified John Deere 90 or 90 plot combine owned by Iowa State University. Weight (pounds) and percent moisture were recorded with either a HarvestMaster brand or Shivvers brand 00 moisture meter and Avery-WeighTronix weigh system/9 weigh scale indicator harvest data collection system. These measurements were converted to bushels per acre of No. shelled corn ( lbs. per bushel) at.% moisture in Microsoft Excel. Data analysis Data were analyzed with analysis of variance (ANOVA) procedures using SAS 9.. When a significant treatment effect was present pairwise comparisons made among means with an experiment wise error rate of P < 0.0. Product consistency: Percent product consistency was calculated as the percentage of times a treatment limited feeding injury to 0. node or less (greater injury can result in economic yield loss, especially when plants are moisture stressed).

10 RESULTS AND DISCUSSION Rootworm populations continued to increase in abundance during 0, after falling to very low levels in 0. A survey of injury to untreated checks at the provides a general sense of larval rootworm pressure during 0. Feeding pressure at the Johnson Farm remained moderate to light with untreated checks suffering between 0. nodes to just under.0 nodes of injury. At the Bruner Farm, untreated checks suffered only around 0. nodes of injury. By contrast, injury to untreated non-rootworm Bt corn at the Southeast Research and Demonstration Farm was over. nodes, and at the Northeast Research and Demonstration Farm ranged from just over 0. nodes to just under.0 nodes. In general, growing conditions were good during 0 and effects of root injury on yield were not detected, although a number of differences among treatments in the level of root injury were observed. The one exception was the Southeast Research and Demonstration Farm where soil-applied insecticide increase yield (Table ). At the Johnson Farm, very good root protection was observed in most studies with either Bt-rootworm corn or soil-applied insecticide on non-rootworm Bt corn. A comparison of multiple Bt products found that all significantly reduced injury compared to the untreated check (Table ). Similar results were found in a second study (Table ). This study also found that the addition of soil-applied insecticide achieved a similar level of root protection when applied to Bt versus non-rootworm Bt corn (Table ). A comparison of several soil-applied insecticides containing the active ingredient (AI) bifenthrin all showed similar and significant levels of root protection (Table ), and similar results were found in a study that compared insecticides with different AIs (Table 8). Interestingly, CryBb corn continues to provide a level of root protection similar to soil-applied insecticides at the Johnson Farm, indicating that CryBb resistance remains rare at this location (Table 9). Due to the low to moderate rootworm pressure at Johnson Farm, some studies yielded very little separation between means (Table and Table ). Results for the Bruner were very similar to the Johnson Farm (Tables to 0). At the Northeast Iowa Research and Demonstration Farm, issues with western corn rootworm resistance to CryBb corn, and cross-resistance to mcrya and ecry.ab, were observed. Both CryBb corn and corn pyramided with mcry and ecry.ab suffered significantly higher injury than corn with Cry/Ab (Table ). Similarly, we found that CryBb corn had higher injury than corn with Cry/Ab or non-bt corn with soil-applied insecticide (Table ). For corn with Cry/Ab and a blended refuge (i.e., non-bt seeds mixed with Bt seeds), the application of soil-applied insecticide led to a decrease in root injury (ca. 0. nodes). However, these differences were not statistically significant. In a study comparing various soil-applied insecticides on nonrootworm Bt corn, all insecticides reduced injury compared to the untreated check, however, differences among insecticides were present (Table ). Aztec, Force and Ampex all led to a greater reduction in injury than Capture, and Aztec reduced injury more than all other insecticides (Table ).

11 AGRONOMIC INFORMATION, WEATHER DATA AND MATERIALS TESTED AND PICTURES OF RESEARCH ACTIVITIES Agronomic information and field history are listed in Appendix I. Weather data from the test site or the nearest Iowa climatological station are listed in Appendix II. Information on materials tested is listed in Appendix III. Pictures of various research activities are in Appendix IV. RESEARCH SUPPORT Support for this research was provided by the Iowa State University Agriculture and Home Economics Experiment Station and by the following companies: AMVAC, Dow AgroSciences, DuPont- Pioneer, FMC, Neudorff, Marrone Bio Innovations, Monsanto, and Valent. WAIVER OF ENDORSEMENT This report deals with the relative ability of each treatment to protect corn from damage by insects. This information is not presented to endorse the use of any product and the name of Iowa State University should not appear in any advertising without prior written consent. Iowa State University, their respective officers, agents, or employees, have not made, and do not hereby make, any representation, warranty or covenant with respect to the use of these test results, nor will they be liable for any damages, losses, or claims, including those of an incidental or consequential nature, arising out of the use of these test results. We would also like to thank Calcium Products (Andrew Hoiberg) for the generous donation of tons of pelletized lime for applying at the ISU Johnson farm to adjust the ph of the field plot soils.

12 8 Table. Iowa evaluation of insecticides and plant-incorporated protectants. Experimental Unit Size Table Entries/ Rows/ Row # Test Location Type of Studies Numbers Test Plot Length (ft) Reps Ames-Johnson Farm Boone-Bruner Farm )Comparison among Bt Corn Traits )AMVAC Aztec-HC Efficacy and Yield Study )FMC Evaluations )FMC Capture RIVED on Bt Corn )FMC Capture RIVED on non-rw Bt Corn )Neudorff Study on non-rw Bt Corn )Valent Ampex Study )Comparison among Bt Corn Traits )Dow AgroSciences Study )FMC Evaluations )FMC Experimental Capture RIVED on Traited Corn All these studies were conducted following corn rootworm trap crop, to ensure the presence of rootworm larvae in the study Crawfordsville ) Valent Ampex Study - 8 Nashua )Comparison among Bt Corn Traits )AMVAC Aztec-HC Efficacy and Yield Study )Marrone Bio Innovations Study )Valent Ampex Study

13 9 Table. Node injury and product consistency in comparison among Bt corn traits: Johnson Farm, Ames Node- Product Treatment Injury,, Consistency 8,9 Agrisure Duracade 0.0a 00a DeKalb VT 0.0ab 9a Pioneer HXX 0.8 b 80a Agrisure, DeKalb, Pioneer non-rw Bt 0.9 c 0 b Planted April, 0; evaluated August 8, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; The following three non-rw Bt seeds were mixed together equally in bag; Agrisure non-rw Bt (Agrisure NH-GTA); DeKalb non-rw Bt (DKC 8-89 VTP RIB); Pioneer non-rw Bt (P088HR); Pioneer HXX = Pioneer HXX (P08HXX); DeKalb VT = DeKalb brand YieldGard VT Triple (DKC 8-8); Agrisure Duracade = Agrisure Duracade Artesian (Agrisure NA-A) Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table. Average root-injury and product consistency for AMVAC Aztec-HC efficacy and yield study: Johnson Farm, Ames. Node- Product Treatment Form. Rate Placement Injury,, Consistency,8 DeKalb VT RIB + Aztec-HC 9.G 0. Furrow-SB 0.0a 9a DeKalb non-rw Bt + Aztec-HC 9.G 0. Furrow-SB 0.0a 00a DeKalb SSTX RIB + Aztec-HC 9.G 0. Furrow-SB 0.0a 00a Pioneer AMX + Aztec-HC 9.G 0. Furrow-SB 0.08a 00a Pioneer AMXT + Aztec-HC 9.G 0. Furrow-SB 0.0a 00a Pioneer non-rw Bt + Aztec-HC 9.G 0. Furrow-SB 0.a 9a DeKalb SSTX RIB a 9a Pioneer AMX a 90a Pioneer AMXT a 8a DeKalb VT RIB a ab DeKalb non-rw Bt a 0ab Pioneer non-rw Bt b b Planted May, 0; evaluated August, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP (DKC 8-89); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC 8-8); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 8-8); Pioneer non-rw Bt = Pioneer brand RR (P9R); Pioneer AMX = Pioneer brand Herculex XTRA (P9AMX); Pioneer AMXT = Pioneer Optimum AcreMax Extreme (P9AMXT) Insecticide listed as ounces a.i. per,000 row-feet Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury Scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less

14 0 Table. Average stand count for AMVAC Aztec-HC efficacy and yield study: Johnson Farm, Ames. Stand Treatment Form. Rate Placement Counts, DeKalb RIB SSTX DeKalb non-rw Bt + Aztec-HC 9.G 0. Furrow-SB. Pioneer non-rw Bt + Aztec-HC 9.G 0. Furrow-SB.0 DeKalb VT RIB Pioneer AMXT DeKalb RIB SSTX + Aztec-HC 9.G 0. Furrow-SB.00 DeKalb VT RIB + Aztec-HC 9.G 0. Furrow-SB.00 DeKalb non-rw Bt Pioneer AMX Pioneer non-rw Bt Pioneer AMX + Aztec-HC 9.G 0. Furrow-SB.00 Pioneer AMXT + Aztec-HC 9.G 0. Furrow-SB.00 Planted May, 0; evaluated May and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP (DKC 8-89); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC 8-8); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 8-8); Pioneer non-rw Bt = Pioneer brand RR (P9R); Pioneer AMX = Pioneer brand Herculex XTRA (P9AMX); Pioneer AMXT = Pioneer Optimum AcreMax Extreme (P9AMXT) Insecticide listed as ounces a.i. per,000 row-feet Furrow-SB = Insecticide applied with SmartBox system at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications x evaluation dates) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table. Average yield for AMVAC Aztec-HC efficacy and yield study: Johnson Farm, Ames. Bushels/ Treatment Form. Rate Placement Acre,, DeKalb VT RIB + Aztec-HC 9.G 0. Furrow-SB 9a DeKalb non-rw Bt a DeKalb VT RIB a DeKalb non-rw Bt + Aztec-HC 9.G 0. Furrow-SB 0a DeKalb RIB SSTX + Aztec-HC 9.G 0. Furrow-SB 9a DeKalb RIB SSTX a Pioneer AMXT + Aztec-HC 9.G 0. Furrow-SB 9 b Pioneer AMXT b Pioneer AMX + Aztec-HC 9.G 0. Furrow-SB 9 b Pioneer non-rw Bt b Pioneer AMX b Pioneer non-rw Bt + Aztec-HC 9.G 0. Furrow-SB 80 b Planted May, 0; machine harvested October 9, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP (DKC 8-89); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC 8-8); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 8-8); Pioneer non-rw Bt = Pioneer brand RR (P9R); Pioneer AMX = Pioneer brand Herculex XTRA (P9AMX); Pioneer AMXT = Pioneer Optimum AcreMax Extreme (P9AMXT) Insecticide listed as ounces a.i. per,000 row-feet Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on observations (-row treatment x 8 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture

15 Table. Average root injury and product consistency for FMC evaluations: Johnson farm, Ames Treatment Form Form. Node- Product Rate Placement Injury,, Consistency 8,9 DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 0.0a 9 DeKalb non-rw Bt + Tepera Plus 0.8SC 0. Furrow 0.0a 00 DeKalb non-rw Bt + Ethos XB.SC 0.0 Furrow 0.0a 9 DeKalb non-rw Bt + Ethos LFR.0SC 0. Furrow 0.0a 00 DeKalb non-rw Bt + Expedient.0SC 0. Furrow 0.0a 9 DeKalb non-rw Bt b 0 Planted May, 0; evaluated August 8, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -98); All treatments included Starter Fertilizer as a carrier Insecticide listed as ounces a.i. per,000 row-feet T-band & In-furrow = insecticide applied at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury Scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9 No significant differences between means (ANOVA, P < 0.0) Table. Stand counts for FMC evaluations: Johnson Farm, Ames Stand Treatment Form. Rate Placement Counts, DeKalb non-rw Bt DeKalb non-rw Bt + Tepera Plus 0.8SC 0. Furrow 0. DeKalb non-rw Bt + Ethos LFR.0SC 0. Furrow 0. DeKalb non-rw Bt + Expedient.0SC 0. Furrow 0. DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 0. DeKalb non-rw Bt + Ethos XB.SC 0.0 Furrow 9.00 Planted May, 0; evaluated May and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -98); All treatments included Starter Fertilizer as a carrier Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications x evaluations) No significant differences between means (ANOVA, P < 0.0)

16 Table 8. Average yield for FMC evaluations: Johnson Farm, Ames Bushels/ Treatment Form. Rate Placement Acre,, DeKalb non-rw Bt + Ethos XB.SC 0.0 Furrow DeKalb non-rw Bt + Tepera Plus 0.8SC 0. Furrow DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 8 DeKalb non-rw Bt + Expedient.0SC 0. Furrow DeKalb non-rw Bt + Ethos LFR.0SC 0. Furrow 0 DeKalb non-rw Bt Planted May, 0; machine harvested October 9, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -98); All treatments included Starter Fertilizer as a carrier Insecticide listed as ounces a.i. per,000 row-feet Furrow = insecticide applied at planting time Chemical and check means based on observations (-row treatment x 9 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture Table 9. Node injury and product consistency for FMC Capture RIVED on Bt corn: Johnson Farm, Ames Node- Product Treatment Form. Rate Placement Injury,, Consistency 8,9 DeKalb VTP RIB + Capture LFR+VGR.SC Furrow 0.09a 9 DeKalb VTP + Force 0CS 0. Furrow 0.09a 9 DeKalb VTP RIB + Ethos XB.SC 0.0 Furrow 0.a 9 DeKalb VTP RIB + Ethos LFR.0SC 0.9 Furrow 0.a 9 DeKalb VTP RIB a 9 DeKalb VTP RIB + Capture LFR.SC 0.0 Furrow 0.a 8 DeKalb VTP RIB + Capture RIVE D.SC 0.0 Furrow 0.a DeKalb non-rw Bt b 0 Planted May, 0; evaluated August 0, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb -98); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC -9); All treatments included Starter Fertilizer as a carrier except the Force and Capture RIVE D treatments which used water as a carrier. All Insecticides listed as ounces a.i. per,000 row-feet except product VGR listed as grams per acre Furrow = insecticide applied at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9 No significant differences between means (ANOVA, P < 0.0)

17 Table 0. Stand counts for FMC Capture RIVED on Bt corn: Johnson Farm, Ames Stand Treatment Form. Rate Placement Counts, DeKalb VTP RIB + Capture RIVE D.SC 0.0 Furrow.00 DeKalb VTP RIB + Ethos XB.SC 0.0 Furrow.00 DeKalb non-rw Bt DeKalb VTP RIB + Force 0CS 0. Furrow.00 DeKalb VTP RIB + Ethos LFR.0SC 0.9 Furrow.0 DeKalb VTP RIB + Capture LFR+VGR.SC Furrow.0 DeKalb VTP RIB + Capture LFR.SC 0.0 Furrow. DeKalb VTP RIB Planted May, 0; evaluated May and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb -98); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC -9); All treatments included Starter Fertilizer as a carrier except the Force and Capture RIVE D treatments which used water as a carrier All Insecticides listed as ounces a.i. per,000 row-feet except product VGR listed as grams per acre Furrow = insecticide applied at planting time Chemical and check means based on observations (-row treatment x. row-feet/treatment x replications x evaluation dates) No significant differences between means (ANOVA, P < 0.0) Table. Yield for FMC Capture RIVED on Bt corn: Johnson Farm, Ames Bushels/ Treatment Form. Rate Placement Acre,, DeKalb VTP RIB R DeKalb VTP RIB + Capture RIVE D.SC 0.0 Furrow DeKalb VTP RIB + Force 0CS 0. Furrow DeKalb non-rw Bt DeKalb VTP RIB + Ethos XB.SC 0.0 Furrow DeKalb VTP RIB + Ethos LFR.0SC 0.9 Furrow DeKalb VTP RIB + Capture LFR+VGR.SC Furrow DeKalb VTP RIB + Capture LFR.SC 0.0 Furrow Planted May, 0; Machine harvested October 9, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb -98); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC -9); All treatments included Starter Fertilizer as a carrier except the Force and Capture RIVE D treatments which used water as a carrier. All Insecticides listed as ounces a.i. per,000 row-feet except product VGR listed as grams per acre Furrow = insecticide applied at planting time Chemical and check means based on observations (-row treatment x row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture

18 Table. Node injury and product consistency for FMC Capture RIVED on non-rw Bt corn: Johnson Farm, Ames. Node- Product Treatment Form. Rate Placement Injury,, Consistency 8,9 DeKalb non-rw Bt + Force 0CS 0. Furrow 0. DeKalb non-rw Bt + Ethos XB.SC 0.0 Furrow 0.8 DeKalb non-rw Bt + Capture LFR+VGR.SC Furrow 0.9 DeKalb non-rw Bt + Capture RIVE D.SC 0.0 Furrow 0. DeKalb VTP RIB DeKalb non-rw Bt + Ethos LFR.0SC 0.9 Furrow 0. 0 DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 0. 0 DeKalb non-rw Bt Planted May, 0; evaluated August, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb -98); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC -9); All treatments included Starter Fertilizer as a carrier except the Force and Capture RIVE D treatments which used water as a carrier. Insecticides listed as ounces a.i. per,000 row-feet Furrow = insecticide applied at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury scale (0-). Number of full or partial nodes completely eaten No significant differences between means (ANOVA, P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9 No significant differences between means (ANOVA, P < 0.0) Table. Stand counts for FMC Capture RIVED on non-rw Bt corn: Johnson Farm, Ames. Stand Treatment Form. Rate Placement Counts, DeKalb non-rw Bt + Capture RIVE D.SC 0.0 Furrow.00a DeKalb non-rw Bt ab DeKalb non-rw Bt + Force 0CS 0. Furrow.0ab DeKalb non-rw Bt + Ethos XB.SC 0.0 Furrow.0ab DeKalb VTP RIB ab DeKalb non-rw Bt + Capture LFR+VGR.SC Furrow.ab DeKalb non-rw Bt + Ethos LFR.0SC 0.9 Furrow.00 b DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow.00 b Planted May, 0; evaluated May and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb -98); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC -9); All treatments included Starter Fertilizer as a carrier except the Force and Capture RIVE D treatments which used water as a carrier Insecticides listed as ounces a.i. per,000 row-feet Furrow = insecticide applied at planting time Chemical and check means based on observations (-row treatment x. row-feet/treatment x replications x evaluation dates) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0)

19 Table. Yield for FMC Capture RIVED on Non-RW Bt corn: Johnson Farm, Ames. ` Bushels/ Treatment Form. Rate Placement Acre,, DeKalb VTP RIB R DeKalb non-rw Bt + Ethos LFR.0SC 0.9 Furrow DeKalb non-rw Bt + Capture RIVE D.SC 0.0 Furrow DeKalb non-rw Bt DeKalb non-rw Bt + Capture LFR+VGR.SC Furrow 9 DeKalb non-rw Bt + Force 0CS 0. Furrow 9 DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 8 DeKalb non-rw Bt + Ethos XB.SC 0.0 Furrow Planted May, 0; Machine harvested October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb -98); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC -9); All treatments included Starter Fertilizer as a carrier except the Force and Capture RIVE D treatments which used water as a carrier. Insecticides listed as ounces a.i. per,000 row-feet Furrow = insecticide applied at planting time Chemical and check means based on observations (-row treatment x row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture Table. Node injury and product consistency for Neudorff study on non-rw Bt corn: Johnson Farm, Ames Node- Product Treatment Form. Rate Placement Injury,, Consistency 8,9 DeKalb non-rw Bt + Force G 0. Furrow 0.a 90 DeKalb non-rw Bt + Antixx Plus lbs/a T-Band 0.ab DeKalb non-rw Bt + Antixx Plus lbs/a Furrow 0.ab DeKalb non-rw Bt ab DeKalb non-rw Bt + Antixx Plus lbs/a Furrow 0. b 0 DeKalb non-rw Bt + Antixx Plus lbs/a T-Band 0. b 0 Planted May, 0; evaluated August 9, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb - 98 RIB VTP) Force Insecticide listed as ounces a.i. per,000 row-feet Furrow & T-Band = insecticide and bait applied at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9 No significant differences between means (ANOVA, P < 0.0)

20 Table. Stand counts for Neudorff study on non-rw Bt corn: Johnson Farm, Ames Stand Treatment Form. Rate Placement Counts, DeKalb non-rw Bt + Antixx Plus lbs/a T-Band. DeKalb non-rw Bt + Force G 0. Furrow.0 DeKalb non-rw Bt + Antixx Plus lbs/a T-Band.00 DeKalb non-rw Bt + Antixx Plus lbs/a Furrow 0.0 DeKalb non-rw Bt + Antixx Plus lbs/a Furrow 0. DeKalb non-rw Bt Planted May, 0; evaluated May and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb - 98 RIB VTP) Force Insecticide listed as ounces a.i. per,000 row-feet Furrow & T-Band = insecticide and bait applied at planting time Chemical and check means based on observations (-row treatment x. row-feet/treatment x replications x evaluation dates) No significant differences between means (ANOVA, P < 0.0) Table. Yield for Neudorff study on non-rw Bt corn: Johnson Farm, Ames Treatment Form. Rate R Bushels/ Placement Acre,, DeKalb non-rw Bt + Antixx Plus lbs/a Furrow 9 DeKalb non-rw Bt + Antixx Plus lbs/a Furrow 8 DeKalb non-rw Bt + Force G 0. Furrow DeKalb non-rw Bt + Antixx Plus lbs/a T-Band 0 DeKalb non-rw Bt DeKalb non-rw Bt + Antixx Plus lbs/a T-Band Planted May, 0; Machine harvested October 9, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb - 98 RIB VTP) Force Insecticide listed as ounces a.i. per,000 row-feet Furrow & T-Band = insecticide and bait applied at planting time Chemical and check means based on observations (-row treatment x 9 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture

21 Table 8. Average root injury and product consistency for Valent Ampex study: Johnson Farm, Ames Node- Product Treatment Form. Rate Placement Injury,, Consistency 8,9 DeKalb non-rw Bt + Aztec-SB.G 0. Furrow-SB 0.0a 00a DeKalb non-rw Bt + Ampex.SC 0.0 Furrow 0.09ab 00a DeKalb non-rw Bt + Force.0G 0. Furrow 0.09ab 00a DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 0. b 80 b DeKalb non-rw Bt c 0 b Planted May, 0; evaluated August 8, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0) Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time; Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury Scale (0-). Number of full or partial nodes completely eaten Chemical and check means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9. No significant differences between means (ANOVA, P < 0.0) Table 9. Stand counts for Valent Ampex study: Johnson Farm, Ames Stand Treatment Form. Rate Placement Counts, DeKalb non-rw Bt + Force.0G 0. Furrow.00 DeKalb non-rw Bt DeKalb non-rw Bt + Ampex.SC 0.0 Furrow. DeKalb non-rw Bt + Aztec-SB.G 0. Furrow-SB. DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow. Planted May, 0; evaluated May and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0) Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time; Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications x evaluations) No significant differences between means (ANOVA, P < 0.0)

22 8 Table 0. Average yields for the Valent Ampex study: Johnson Farm, Ames Bushels/ Treatment Form. Rate Placement Acre,, DeKalb VT + Capture LFR.SC 0.0 Furrow 9 DeKalb VT + Force.0G 0. Furrow 8 DeKalb VT + Aztec-SB.G 0. Furrow-SB DeKalb VT + Ampex.SC 0.0 Furrow DeKalb VT Planted May, 0; machine harvested October 9, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0) Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time; Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on observations (-rows/ treatment x 9 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture Table. Node injury and product consistency in comparison among Bt traits study: Bruner Farm, Boone Node- Product Treatment Injury,, Consistency, Agrisure Duracade 0.a 90a DeKalb VT 0.a 9a Pioneer HXX 0.a 9a Agrisure, DeKalb, Pioneer non-rw Bt 0. b 0 b Planted May, 0; evaluated August, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; The following three non-rw Bt seeds were mixed together equally in bag; Agrisure non-rw Bt (Agrisure NH-GTA); DeKalb non-rw Bt (DKC 8-89 VTP RIB); Pioneer non-rw Bt (P088HR); Pioneer HXX = Pioneer HXX (P08HXX); DeKalb VT = DeKalb brand YieldGard VT Triple (DKC 8-8); Agrisure Duracade = Agrisure Duracade Artesian (Agrisure NA-A) Means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0)

23 9 Table. Average root injury and product consistency for the Dow AgroSciences study: Bruner Farm, Boone Node- Product Treatment Injury,, Consistency, Mycogen Smartstax 0.0a 00 Mycogen non-rw Bt 0. b 8 Planted May, 0; evaluated August, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; Mycogen non-rw Bt = Mycogen brand (Mycogen A-base genetics); Mycogen Smartstax = Mycogen brand Smartstax (Mycogen A) Check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury Scale (0-). Number of full or partial nodes completely eaten Chemical and check means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less. No significant differences between means (ANOVA, P < 0.0) Table. Stand counts for the Dow AgroSciences study: Bruner Farm, Boone Stand Treatment Counts, Mycogen non-rw Bt.0 Mycogen Smartstax 9. Planted May, 0; evaluated June and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; Mycogen non-rw Bt = Mycogen brand (Mycogen A-base genetics); Mycogen Smartstax = Mycogen brand Smartstax (Mycogen A) Check means based on observations ( rows/ treatment x. row-ft/treatment x replications x evaluations) No significant differences between means (ANOVA, P < 0.0) Table. Average yields for the Dow AgoSciences study: Bruner Farm, Boone Bushels/ Treatment Acre,, Mycogen non-rw Bt Mycogen Smartstax Planted May, 0; machine harvested October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; Mycogen non-rw Bt = Mycogen brand (Mycogen A-base genetics); Mycogen Smartstax = Mycogen brand Smartstax (Mycogen A) Check means based on observations (-rows/ treatment x 9 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture

24 0 Table. Average root injury and product consistency for FMC evaluations; Bruner farm, Boone Node- Product Treatment Form. Rate Placement Injury,, Consistency 8,9 DeKalb non-rw Bt + Ethos LFR.0SC 0. Furrow 0.09a 90 DeKalb non-rw Bt + Ethos XB.SC 0.0 Furrow 0.0a 00 DeKalb non-rw Bt + Expedient.0SC 0. Furrow 0.0a 90 DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 0.0a 9 DeKalb non-rw Bt + Tepera Plus 0.8SC 0. Furrow 0.a 90 DeKalb non-rw Bt b 0 Planted May, 0; evaluated August, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -98); All treatments included Starter Fertilizer as a carrier Insecticide listed as ounces a.i. per,000 row-feet Furrow = insecticide applied at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury Scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9 No significant differences between means (ANOVA, P < 0.0) Table. Stand counts for FMC evaluations: Bruner Farm, Boone Stand Treatment Form. Rate Placement Counts, DeKalb non-rw Bt + Expedient.0SC 0. Furrow. DeKalb non-rw Bt + Tepera Plus 0.8SC 0. Furrow 0.0 DeKalb non-rw Bt + Ethos XB.SC 0.0 Furrow 0.0 DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 0.00 DeKalb non-rw Bt + Ethos LFR.0SC 0. Furrow 0.00 DeKalb non-rw Bt Planted May, 0; evaluated May and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -98); All treatments included Starter Fertilizer as a carrier Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications x evaluations) No significant differences between means (ANOVA, P < 0.0)

25 Table. Average yield for FMC evaluations: Bruner Farm, Boone. Bushels/ Treatment Form. Rate Placement Acre,, DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow DeKalb non-rw Bt + Ethos LFR.0SC 0. Furrow DeKalb non-rw Bt + Tepera Plus 0.8SC 0. Furrow 0 DeKalb non-rw Bt + Ethos XB.SC 0.0 Furrow DeKalb non-rw Bt + Expedient.0SC 0. Furrow DeKalb non-rw Bt Planted May, 0; machine harvested October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -98); All treatments included Starter Fertilizer as a carrier Insecticide listed as ounces a.i. per,000 row-feet Furrow = insecticide applied at planting time Chemical and check means based on observations (-row treatment x 9 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture Table 8. Node injury and product consistency for FMC Capture RIVED on Bt corn: Bruner Farm, Boone Node- Product Treatment Form. Rate Placement Injury,, Consistency 8,9 DeKalb VTP RIB + Force 0CS 0. Furrow 0.0a 00a DeKalb VTP RIB + Ethos XB.SC 0.0 Furrow 0.0a 00a DeKalb VTP RIB + Ethos LFR.0SC 0.9 Furrow 0.0a 00a DeKalb VTP RIB + Capture RIVE D.SC 0.0 Furrow 0.08a 00a DeKalb VTP RIB a 00a DeKalb VTP RIB + Capture LFR.SC 0.0 Furrow 0.09a 9a DeKalb VTP RIB + Capture LFR+VGR.SC Furrow 0.0a 9a DeKalb non-rw Bt b b Planted May, 0 evaluated August, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb -98); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC -9); All treatments included Starter Fertilizer as a carrier except the Force and Capture RIVE D treatments which used water as a carrier All Insecticides listed as ounces a.i. per,000 row-feet except product VGR listed as grams per acre Furrow = insecticide applied at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9 Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0)

26 Table 9. Stand counts for FMC Capture RIVED on Bt corn: Bruner Farm, Boone Stand Treatment Form. Rate Placement Counts, DeKalb non-rw Bt a DeKalb VTP RIB b DeKalb VTP RIB + Capture LFR.SC 0.0 Furrow.00 b DeKalb VTP RIB + Capture LFR+VGR.SC Furrow.00 b DeKalb VTP RIB + Ethos XB.SC 0.0 Furrow.00 bc DeKalb VTP RIB + Ethos LFR.0SC 0.9 Furrow.00 c DeKalb VTP RIB + Force 0CS 0. Furrow. c DeKalb VTP RIB + Capture RIVE D.SC 0.0 Furrow. c Planted May, 0; evaluated June and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb -98); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC -9); All treatments included Starter Fertilizer as a carrier except the Force and Capture RIVE D treatments which used water as a carrier All Insecticides listed as ounces a.i. per,000 row-feet except product VGR listed as grams per acre Furrow = insecticide applied at planting time Chemical and check means based on observations (-row treatment x. row-feet/treatment x replications x evaluation dates) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 0. Yield for FMC Capture RIVED on Bt corn: Bruner Farm, Boone Treatment Form. Rate R Bushels/ Placement Acre,, DeKalb VTP RIB + Ethos LFR.0SC 0.9 Furrow DeKalb VTP RIB + Capture LFR+VGR.SC Furrow DeKalb VTP RIB + Capture LFR.SC 0.0 Furrow 9 DeKalb VTP RIB DeKalb VTP RIB + Ethos XB.SC 0.0 Furrow DeKalb non-rw Bt DeKalb VTP RIB + Capture RIVE D.SC 0.0 Furrow DeKalb VTP RIB + Force 0CS 0. Furrow 0 8 Planted May, 0; Machine harvested October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP RIB (DeKalb -98); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC -9); All treatments included Starter Fertilizer as a carrier except the Force and Capture RIVE D treatments which used water as a carrier All Insecticides listed as ounces a.i. per,000 row-feet except product VGR listed as grams per acre Furrow = insecticide applied at planting time Chemical and check means based on observations (-row treatment x row-feet/treatment x replications) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Yields converted to.% Moisture 8 These mean data based on observations (-row treatment x row-feet/treatment x replications)

27 Table. Average root injury and product consistency for the Valent Ampex study: Southeast Iowa Research and Demonstration Farm, Crawfordsville Node- Product Treatment Form. Rate Placement Injury,, Consistency 8,9 DeKalb non-rw Bt + Ampex.SC 0. Furrow 0.a a DeKalb non-rw Bt b b Planted April, 0; evaluated July 8, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0). Both treatments included Starter Fertilizer as a carrier. Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time Chemical and check means based on 0 observations (0 roots/ rows x replications) Iowa State Node-Injury Scale (0-). Number of full or partial nodes completely eaten Chemical and check means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9. Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table. Stand counts for the Valent Ampex study: Southeast Iowa Research and Demonstration Farm, Crawfordsville Stand Treatment Form. Rate Placement Counts, DeKalb non-rw Bt + Ampex.SC 0. Furrow 9.00 DeKalb non-rw Bt Planted April, 0; evaluated May and November, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0). Both treatments included Starter Fertilizer as a carrier. Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications x evaluations) No significant differences between means (ANOVA, P < 0.0)

28 Table. Average lodging for the Valent Ampex study: Southeast Iowa Research and Demonstration Farm, Crawfordsville Treatment Form. Rate Placement Lodging, DeKalb non-rw Bt + Ampex.SC 0. Furrow DeKalb non-rw Bt Planted April, 0; evaluated November, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0). Both treatments included Starter Fertilizer as a carrier. Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time Chemical and check means based on observations (8 rows/ treatment x. row-ft/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Table. Average yields for the Valent Ampex study: Southeast Iowa Research and Demonstration Farm, Crawfordsville Bushels/ Treatment Form. Rate Placement Acre,, DeKalb non-rw Bt + Ampex.SC 0. Furrow 90a DeKalb non-rw Bt b Planted April, 0; machine harvested November, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0). Both treatments included Starter Fertilizer as a carrier. Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time Chemical and check means based on observations (-rows/ treatment x 0 row-feet/treatment x replications) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Yields converted to.% Moisture

29 Table. Node injury and product consistency for comparison among Bt traits: Northeast Iowa Research and Demonstration Farm, Nashua Node- Product Treatment Injury,, Consistency, Pioneer HXX 0.0a 00a Agrisure Duracade 0. b 0a DeKalb VT 0. b 0ab Agrisure, DeKalb, Pioneer non-rw Bt. c 0 b Planted April, 0; evaluated August, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; The following three non-rw Bt seeds were mixed together equally in bag; Agrisure non-rw Bt (Agrisure NH-GTA); DeKalb non-rw Bt (DKC 8-89 VTP RIB); Pioneer non-rw Bt (P088HR); Pioneer HXX = Pioneer HXX (P08HXX); DeKalb VT = DeKalb brand YieldGard VT Triple (DKC 8-8); Agrisure Duracade = Agrisure Duracade Artesian (Agrisure NA-A) Means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table. Average root-injury and product consistency for AMVAC Aztec-HC efficacy and yield study: Northeast Iowa Research and Demonstration Farm, Nashua Node- Product Treatment Form. Rate Placement Injury,, Consistency,8 Pioneer AMX + Aztec-HC 9.G 0. Furrow-SB 0.0a 00a DeKalb RIB SSTX + Aztec-HC 9.G 0. Furrow-SB 0.0a 00a Pioneer AMXT + Aztec-HC 9.G 0. Furrow-SB 0.ab 9a Pioneer AMX abc 90a DeKalb VT RIB + Aztec-HC 9.G 0. Furrow-SB 0.abc 80a DeKalb RIB SSTX abc 90a Pioneer non-rw Bt + Aztec-HC 9.G 0. Furrow-SB 0.abc 8a Pioneer AMXT bcd 0ab DeKalb non-rw Bt + Aztec-HC 9.G 0. Furrow-SB 0.8 cd 0abc DeKalb VT RIB d abc DeKalb non-rw Bt e bc Pioneer non-rw Bt e 0 c Planted May, 0; evaluated August, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP (DKC 8-89); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC 8-8); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 8-8); Pioneer non-rw Bt = Pioneer brand RR (P9R); Pioneer AMX = Pioneer brand Herculex XTRA (P9AMX); Pioneer AMXT = Pioneer Optimum AcreMax Extreme (P9AMXT) Insecticide listed as ounces a.i. per,000 row-feet Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury Scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less

30 Table. Average stand count for AMVAC Aztec-HC efficacy and yield study: Northeast Iowa Research and Demonstration Farm, Nashua Stand Treatment Form. Rate Placement Counts, Pioneer non-rw Bt + Aztec-HC 9.G 0. Furrow-SB.00a Pioneer non-rw Bt a DeKalb RIB SSTX + Aztec-HC 9.G 0. Furrow-SB.00a DeKalb non-rw Bt + Aztec-HC 9.G 0. Furrow-SB.00a Pioneer AMXT + Aztec-HC 9.G 0. Furrow-SB.a DeKalb non-rw Bt ab Pioneer AMX ab Pioneer AMXT ab DeKalb RIB SSTX ab Pioneer AMX + Aztec-HC 9.G 0. Furrow-SB 9.0abc DeKalb VT RIB + Aztec-HC 9.G 0. Furrow-SB 8.00 bc DeKalb VT RIB c Planted May, 0; evaluated June and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP (DKC 8-89); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC 8-8); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 8-8); Pioneer non-rw Bt = Pioneer brand RR (P9R); Pioneer AMX = Pioneer brand Herculex XTRA (P9AMX); Pioneer AMXT = Pioneer Optimum AcreMax Extreme (P9AMXT) Insecticide listed as ounces a.i. per,000 row-feet Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications x evaluation dates) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) Table 8. Average lodging for AMVAC Aztec-HC efficacy and yield study: Northeast Iowa Research and Demonstration Farm, Nashua Treatment Form. Rate Placement Lodging, DeKalb RIB SSTX a Pioneer AMX + Aztec-HC 9.G 0. Furrow-SB 0a Pioneer AMXT + Aztec-HC 9.G 0. Furrow-SB 0a DeKalb RIB SSTX + Aztec-HC 9.G 0. Furrow-SB ab Pioneer AMX ab Pioneer non-rw Bt + Aztec-HC 9.G 0. Furrow-SB ab Pioneer AMXT ab DeKalb VT RIB + Aztec-HC 9.G 0. Furrow-SB ab DeKalb non-rw Bt + Aztec-HC 9.G 0. Furrow-SB ab DeKalb VT RIB ab Pioneer non-rw Bt ab DeKalb non-rw Bt b Planted May, 0; evaluated October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP (DKC 8-89); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC 8-8); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 8-8); Pioneer non-rw Bt = Pioneer brand RR (P9R); Pioneer AMX = Pioneer brand Herculex XTRA (P9AMX); Pioneer AMXT = Pioneer Optimum AcreMax Extreme (P9AMXT) Insecticide listed as ounces a.i. per,000 row-feet Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on 8 observations ( rows/ treatment x. row-ft/treatment x replications) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0)

31 Table 9. Average yield for AMVAC Aztec-HC efficacy and yield study: Northeast Iowa Research and Demonstration Farm, Nashua Bushels/ Treatment Form. Rate Placement Acre,, DeKalb non-rw Bt + Aztec-HC 9.G 0. Furrow-SB DeKalb RIB SSTX + Aztec-HC 9.G 0. Furrow-SB 0 DeKalb RIB SSTX DeKalb VT RIB + Aztec-HC 9.G 0. Furrow-SB 0 DeKalb non-rw Bt Pioneer AMXT + Aztec-HC 9.G 0. Furrow-SB 00 DeKalb VT RIB Pioneer AMX Pioneer AMX + Aztec-HC 9.G 0. Furrow-SB 9 Pioneer AMXT Pioneer non-rw Bt + Aztec-HC 9.G 0. Furrow-SB 8 Pioneer non-rw Bt Planted May, 0; machine harvested October 8, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTP (DKC 8-89); DeKalb VTP RIB = DeKalb brand YieldGard VT Triple Pro RIB (DKC 8-8); DeKalb SSTX RIB = DeKalb brand Smartstax RIB (DKC 8-8); Pioneer non-rw Bt = Pioneer brand RR (P9R); Pioneer AMX = Pioneer brand Herculex XTRA (P9AMX); Pioneer AMXT = Pioneer Optimum AcreMax Extreme (P9AMXT) Insecticide listed as ounces a.i. per,000 row-feet Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on observations (-row treatment x 9 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture Table 0. Average root-injury and product consistency for Marrone Bio Innovations study: Northeast Iowa Research and Demonstration Farm, Nashua Node- Product Treatment Form. Rate Placement Injury,, Consistency 8,9 Pioneer non-rw Bt + Venerate XC Furrow 0.a Pioneer non-rw Bt + MBI-0BMSE Furrow 0.8ab Pioneer non-rw Bt + Venerate XC Furrow 0.abc Pioneer non-rw Bt + Capture LFR.SC 0.0 Furrow 0. bc 0 Pioneer non-rw Bt c 0 Planted April, 0; evaluated August &, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; Pioneer non-rw Bt = Pioneer brand RR (P08HR) Capture LFR Insecticide listed as ounces a.i. per,000 row-feet. Venerate XC & MBI-0BMSE products listed as gallon per Acre Furrow = insecticide applied at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury Scale (0-). Number of full or partial nodes completely eaten Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9 No significant differences between means (ANOVA, P < 0.0)

32 8 Table. Average stand count for Marrone Bio Innovations study: Northeast Iowa Research and Demonstration Farm, Nashua Stand Treatment Form. Rate Placement Counts, Pioneer non-rw Bt + Venerate XC Furrow.0 Pioneer non-rw Bt + Capture LFR.SC 0.0 Furrow 0.0 Pioneer non-rw Bt Pioneer non-rw Bt + Venerate XC Furrow 0.00 Pioneer non-rw Bt + MBI-0BMSE Furrow 0.00 Planted April, 0; evaluated June and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; Pioneer non-rw Bt = Pioneer brand RR (P08HR) Capture LFR Insecticide listed as ounces a.i. per,000 row-feet. Venerate XC and MBI-0BMSE products listed as gallon per Acre Furrow = Insecticide applied at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications x evaluation dates) No significant differences between means (ANOVA, P < 0.0) Table. Average lodging for Marrone Bio Innovations study: Northeast Iowa Research and Demonstration Farm, Nashua Treatment Form. Rate Placement Lodging, Pioneer non-rw Bt + MBI-0BMSE Furrow Pioneer non-rw Bt + Venerate XC Furrow Pioneer non-rw Bt + Venerate XC Furrow Pioneer non-rw Bt Pioneer non-rw Bt + Capture LFR.SC 0.0 Furrow Planted April, 0; evaluated October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; Pioneer non-rw Bt = Pioneer brand RR (P08HR) Capture LFR Insecticide listed as ounces a.i. per,000 row-feet. Venerate XC & MBI-0BMSE products listed as gallon per Acre Furrow = Insecticide applied at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications) No significant differences between means (ANOVA, P < 0.0)

33 9 Table. Average yield for Marrone Bio Innovations study: Northeast Iowa Research and Demonstration Farm, Nashua Bushels/ Treatment Form. Rate Placement Acre,, Pioneer non-rw Bt + Venerate XC Furrow Pioneer non-rw Bt Pioneer non-rw Bt + Capture LFR.SC 0.0 Furrow 8 Pioneer non-rw Bt + Venerate XC Furrow Pioneer non-rw Bt + MBI-0BMSE Furrow Planted April, 0; machine harvested October 8, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; Pioneer non-rw Bt = Pioneer brand RR (P08HR) Capture LFR Insecticide listed as ounces a.i. per,000 row-feet. Venerate XC & MBI-0BMSE products listed as gallon per Acre Furrow = insecticide applied at planting time Chemical and check means based on observations (-row treatment x 9 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture Table. Average root injury and product consistency for the Valent Ampex study: Northeast Iowa Research and Demonstration Farm, Nashua Node- Product Treatment Form. Rate Placement Injury,, Consistency 8,9 DeKalb non-rw Bt + Aztec-SB.G 0. Furrow-SB 0.0a 00a DeKalb non-rw Bt + Force.0G 0. Furrow 0. b ab DeKalb non-rw Bt + Ampex.SC 0.0 Furrow 0.8 b 0ab DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 0. c b DeKalb non-rw Bt d 0 c Planted April, 0; evaluated August &, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0) Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time; Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on 0 observations ( roots/ rows x replications) Iowa State Node-Injury Scale (0-). Number of full or partial nodes completely eaten Chemical and check means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0) 8 Product consistency = Percentage of times nodal injury was 0. (¼ node eaten) or less 9. Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0)

34 0 Table. Stand counts for the Valent Ampex study: Northeast Iowa Research and Demonstration Farm, Nashua Stand Treatment Form. Rate Placement Counts, DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow.00 DeKalb non-rw Bt + Force.0G 0. Furrow 0. DeKalb non-rw Bt + Ampex.SC 0.0 Furrow 0. DeKalb non-rw Bt DeKalb non-rw Bt + Aztec-SB.G 0. Furrow-SB 9.00 Planted April, 0; evaluated June and October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0) Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time; Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications x evaluations) No significant differences between means (ANOVA, P < 0.0) Table. Average lodging for Valent Ampex study: Northeast Iowa Research and Demonstration Farm, Nashua Treatment Form. Rate Placement Lodging, DeKalb non-rw Bt + Aztec-SB.G 0. Furrow-SB 0a DeKalb non-rw Bt + Force.0G 0. Furrow 0a DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 0a DeKalb non-rw Bt + Ampex.SC 0.0 Furrow a DeKalb non-rw Bt b Planted April, 0; evaluated October, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0) Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time; Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on observations ( rows/ treatment x. row-ft/treatment x replications) Means sharing a common letter do not differ significantly according to Ryan s Q Test (P < 0.0)

35 Table. Average yields for the Valent Ampex study: Northeast Iowa Research and Demonstration Farm, Nashua Bushels/ Treatment Form. Rate Placement Acre,, DeKalb non-rw Bt + Force.0G 0. Furrow 9 DeKalb non-rw Bt + Aztec-SB.G 0. Furrow-SB 9 DeKalb non-rw Bt DeKalb non-rw Bt + Ampex.SC 0.0 Furrow DeKalb non-rw Bt + Capture LFR.SC 0.0 Furrow 9 Planted April, 0; machine harvested October 8, 0 non-rw Bt = an absence of any Bt trait targeting corn rootworm; DeKalb non-rw Bt = DeKalb brand VTPro RIB (DKC -0) Insecticide listed as ounces a.i. per,000 row-feet Furrow = Insecticide applied at planting time; Furrow-SB = insecticide applied with SmartBox system at planting time Chemical and check means based on observations (-rows/ treatment x 9 row-feet/treatment x replications) No significant differences between means (ANOVA, P < 0.0) Yields converted to.% Moisture

36 APPENDIX I Agronomic Information

37 0 Field History Data Ames, IA-ISU Johnson Farm Corn Rootworm studies (following trap crop) Boone, IA-ISU Bruner Farm Corn Rootworm studies (following trap crop) Insecticide History 0 No Insecticide (trap crop) Corn (Trap crop) 0 Insecticide Test Plot Corn (Insecticide Test Plot) 0 No Insecticide (trap crop) Corn 0 Insecticide Test Plot Corn Tillage Variety Fall 0-disk ripper ( inch) Spring 0-field cultivation (X) DeKalb DKC 8-8 VT, DeKalb DKC 8-89 VTP RIB, Pioneer P088HR, Pioneer P08HXX, Agrisure NH- GTA, Agrisure NA-A, DeKalb DKC -98 VTP RIB, DKC -9 VTP RIB, DKC -0 VTP RIB Fall 0-chopped stalks & Chisel plow; Spring 0-field cultivation (X) DeKalb DKC 8-8 VT, DeKalb DKC 8-89 VTP RIB, Pioneer P088HR, Pioneer P08HXX, Agrisure NH-GTA, Agrisure NA-A, DeKalb DKC -98 VTP RIB, DKC -9 VTP RIB Mycogen A Planting Date April, May,,, May Planting Rate,00 seeds/a,00 seeds/a Herbicides. pts. Surestart + 0 oz. Harness-April -PRE; oz. Capreno + COC-June -POST. oz. Corvus + UAN-May - PRE; 0. oz. Impact +. oz. Roundup-June -POST;. oz. Laudis-June -POST Fertilizer N P K S Zn N P K S Zn Fall/0 Preplant Fall/ Applied 000 ecce lbs (actual) of 9% ecce pelletized lime Dates Stand Count May & ; October & June ; October Root Digging August & 0 August Lodging October & October Harvest October 9 October Soil Type Clay loam & loam Silty clay loam Soil Organic Matter %.. Soil ph.. Expressed as formulation per acre. Expressed as pounds per acre. Fall/0 applied- lbs. Nitrogen, 0 lbs. Phosphorus, 0 lbs. Potassium, lbs. Sulfur,. lbs. Zinc applied on November, 0 at ISU Johnson Farm (Ames); Spring-preplant 90 lbs., Liquid UAN applied on April at ISU Johnson Farm (Ames); Fall/0 applied- 8 lbs. Nitrogen, 0 lbs. Phosphorus, lbs. Sulfur,. lbs. Zinc applied on November 9, 0 at ISU Bruner Farm (Boone); Spring- Spring-preplant 9 lbs., UAN applied on May at ISU Bruner Farm (Boone); Spring-preplant 0 lbs., dry potash fertilizer applied on March at ISU Bruner Farm (Boone);

38 0 Field History Data Crawfordsville, IA-ISU Southeast Farm Corn Rootworm studies (following trap crop) Nashua, IA-ISU Northeast Farm Corn Rootworm studies (following trap crop) Insecticide History 0 No Insecticide (trap crop) No Insecticide (trap crop) 0 Insecticide Test Plot Insecticide Test Plot 0 No Insecticide (trap crop) No Insecticide (trap crop) 0 Insecticide Test Plot Insecticide Test Plot Tillage Fall 0-chisel plow; Spring 0-field cultivation (X) Fall 0-chisel plow; Spring 0-field cultivation (X) Variety DeKalb DKC -0 VTP RIB DeKalb DKC 8-89 VTP RIB, DeKalb DKC 8-8 VTP RIB, DeKalb DKC 8-8 SSTX RIB, Pioneer P9R, Pioneer P9AMX, Pioneer P9AMXT, Pioneer P08HR, DeKalb DKC -0 VTP RIB, Pioneer P08HXX, Agrisure NH-GTA, Agrisure NA-A Planting Date(s) April April Planting Rate,00 seeds/a,00 seeds/a Herbicides oz. Zidua +. lbs. Atrazine-May -PRE; pts. Halex GT + Atrazine 0. lb.-june -POST. pts. Guardsman Max-April 8- PRE; oz. Roundup WeatherMax- June 0-POST Fertilizer N P K N P K Preplant Dates Stand Count May ; November June ; October Root Digging August August Lodging November October Harvest November October 8 Soil Type Silty Clay Loam Loam Soil Organic Matter %..9 Soil ph.9. Expressed as formulation per acre. Expressed as pounds per acre. Spring-Preplant 00 lbs. per acre actual nitrogen applied as anhydrous ammonia (8.-0-0) on April at Crawfordsville. Spring-Preplant 00 lbs. per acre actual nitrogen applied as anhydrous ammonia (8.-0-0) on April 0 at Nashua.

39 APPENDIX II Weather Data

40 Ames & Boone 0 Rainfall and Temperature April May June Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day Low High Inches Low High Inches Low High Inches Mean/Total Normal D.F.N Weather station located miles WSW of Ames at ISU AEA/Agronomy Farm. Low/High Temperatures & Rainfall amounts for Ames & Boone from Mesonet Weather Station website at:

41 Ames & Boone 0 Rainfall and Temperature July August September Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day Low High Inches Low High Inches Low High Inches Mean/Total Normal D.F.N Weather station located miles WSW of Ames at ISU AEA/Agronomy Farm.

42 8 Ames & Boone 0 Rainfall and Temperature October Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day Low High Inches Low High Inches Low High Inches Mean/Total.9 0. Normal.. D.F.N.. -. Weather station located miles WSW of Ames at ISU AEA/Agronomy Farm.

43 9 Crawfordsville 0 Rainfall and Temperature April May June Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day Low High Inches Low High Inches Low High Inches Mean/Total Normal D.F.N Weather station located at study site. Rainfall amounts manually recorded at study site. Low/High Temperatures for Crawfordsville from Mesonet Weather Station website at:

44 0 Crawfordsville 0 Rainfall and Temperature July August September Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day Low High Inches Low High Inches Low High Inches Mean/Total Normal D.F.N Weather station located at test site. Rainfall amounts manually recorded at study site.

45 Crawfordsville 0 Rainfall and Temperature October November Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day Low High Inches Low High Inches Low High Inches Mean/Total Normal D.F.N Weather station located at test site. Rainfall amounts manually recorded at study site.

46 Nashua 0 Rainfall and Temperature April May June Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day Low High Inches Low High Inches Low High Inches Tr-Rn Tr-Sn Frost Tr-Rn Tr-Rn Tr-Rn Tr-Rn Mean/Total Normal D.F.N Weather station located at test site. Rainfall amounts manually recorded at study site. Low/High Temperatures for Nashua from Mesonet Weather Station website at:

47 Nashua 0 Rainfall and Temperature July August September Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day Low High Inches Low High Inches Low High Inches Tr-Rn Tr-Rn Tr-Rn Mean/Total Normal D.F.N Weather station located at Study site. Rainfall amounts manually recorded at study site.

48 Nashua 0 Rainfall and Temperature October Temp ( F) Rainfall Temp ( F) Rainfall Temp ( F) Rainfall Day Low High Inches Low High Inches Low High Inches Mean/Total.. Normal 9..0 D.F.N Weather station located at test site. Rainfall amounts manually recorded at study site.

49 APPENDIX III Materials Tested

50 Table. Conventional insecticides used in studies in 0 Product name Rate (ounces a.i. per 000 row-feet; unless specified) Active ingredient Company Ampex.SC 0.0 clothianidin Valent Corporation Antixx Plus & 0 lbs/a Iron phosphate Neudorff Aztec-SB.G 0. tebupirimphos & cyfluthrin AMVAC Chem. Corp. Aztec-HC 9.G 0. tebupirimphos & cyfluthrin AMVAC Chem. Corp. Capture RIVE D.SC 0.0 bifenthrin FMC Corporation Capture LFR.SC 0.0 bifenthrin FMC Corporation AMVAC Chem. Expedient 0. bifenthrin Corp. Ethos LFR.0SC 0.9 bifenthrin FMC Corporation Ethos XB.SC 0.0 bifenthrin FMC Corporation Force G 0. tefluthrin Syngenta Force 0CS 0. & 0. tefluthrin Syngenta Tepera Plus 0.0 bifenthrin FMC Corporation Heat-killed Burkholderia spp. strain A9 Marrone Bio Venerate XC 0. &.0 Gallon/Acre cells and spent fermentation media Innovations

51 Table. Rootworm active Bt Traits Product name Events targeting rootworm Toxin Company Agrisure 000GT Agrisure MIR0 mcrya Syngenta Agrisure DAS-9- + MIR0 Cry/Ab + mcrya Syngenta Agrisure A MIR0 + 0 mcrya + ecry.ab Syngenta Herculex DAS-9- Cry/Ab Pioneer & Dow AgroSciences Pioneer AMX DAS-9- Cry/Ab DuPont-Pioneer Pioneer AMXT DAS-9- + MIR0 Cry/Ab + mcrya DuPont-Pioneer Pioneer OAM DAS-9- Cry/Ab DuPont-Pioneer Smartstax DAS-9- + MON 880 Cry/Ab + CryBb Dow AgroSciences & Monsanto VT Triple MON 880 CryBb Monsanto

52 8 APPENDIX IV Research Pictures

53 9 Field crew for 0 Evaluating corn root systems for corn rootworm larvae feeding following the Iowa State Node-Injury Scale (0-)

54 0 Field research personnel evaluating seed corn maggot study at the ISU Johnson farm Screen houses installed over corn plots at the ISU Bruner Farm in Boone, IA. Screen houses are used to collect corn rootworm beetles from traited and non-rw Bt corn.